FvH4_3g33131

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0021052
Physical Location & Seq
Forward (+)
28566724 .. 28568500
1777 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g33131.t1

Sequence Viewer

Length: 558 bp
ATGGTGATGATGAAGTACGATGACATGGTGCAAATGAATATTGTTGAACTCAACGCTCTCTACTTTTGGGTGCGCATCACAAAAGTCCCATCTTTGTTCGAGGAACTTGATACAATCAAGGACATTGCATCTTCTAATGGCAATTTCATTCAACTTGATGAGAAGGTGTTTGAAAATATAGGCAGGGTAACGCTTGTGCATAAAGTCCACAATCCTACTCCTACTCCGACCTCCAAAAGTTACCCAGATCTTATAAAGTGGCACCCGCCTCCCCAAGGTGTAGTCAAGCTATATTTCGATGGTTTTGTGCTTCCAAATCAAGGTGTTGTTGCCGGCTTTGTTATTCGTGATTGCAATGAATGTGCTGCTCTAAAGGATGGTTTAAAAGTTGCTTGCTCGTTTGGCTATAAAAGTATTCAAGTCGAGGGTGATTCTTCTCTCATTATTAACTGTGTCCAAGGCAGTTGGGATATCCCATGGCGTATAAAGTCGCTCATTTCAGACATCGTTGCTGAGTCGGCTATATTTGAAGATGTTTCATTCAGTAATATTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

186

Amino Acids

20.77

Weight (kDa)

5.0

Isoelectric Point (pI)

30.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 120 - 184 7.2e-08 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021052)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g33131
rosa_multiflora Rmu_sc0000903.1_g000032 Rmu_sc0001211.1_g000125 Rmu_sc0003846.1_g000007 Rmu_sc0007066.1_g000010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 254
Acc16I TGCGCA 1 cut(s) 74
AccB1I GGYRCC 1 cut(s) 261
AciI CCGC 1 cut(s) 266
AfaI GTAC 1 cut(s) 17
AfiI CCNNNNNNNGG 2 cut(s) 275, 320
AgsI TTSAA 5 cut(s) 47, 152, 173, 419, 530
AluBI AGCT 1 cut(s) 289
AluI AGCT 1 cut(s) 289
ApeKI GCWGC 1 cut(s) 365
AspLEI GCGC 1 cut(s) 75
AsuHPI GGTGA 2 cut(s) 16, 440
BanI GGYRCC 1 cut(s) 261
BbvI GCAGC 1 cut(s) 352
BccI CCATC 3 cut(s) 97, 293, 371
BglII AGATCT 1 cut(s) 247
BisI GCNGC 1 cut(s) 366
BlsI GCNGC 1 cut(s) 367
BmiI GGNNCC 1 cut(s) 263
BmsI GCATC 2 cut(s) 84, 137
BsaJI CCNNGG 3 cut(s) 274, 457, 476
BsaXI ACNNNNNCTCC 2 cut(s) 208, 238
Bsc4I CCNNNNNNNGG 2 cut(s) 275, 320
Bse118I RCCGGY 1 cut(s) 332
Bse3DI GCAATG 2 cut(s) 123, 361
BseDI CCNNGG 3 cut(s) 274, 457, 476
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 2 cut(s) 275, 320
BseMI GCAATG 2 cut(s) 123, 361
BseMII CTCAG 1 cut(s) 504
BseXI GCAGC 1 cut(s) 352
BshNI GGYRCC 1 cut(s) 261
BsiSI CCGG 1 cut(s) 333
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 2 cut(s) 275, 320
BsmFI GGGAC 1 cut(s) 71
Bsp143I GATC 1 cut(s) 247
Bsp19I CCATGG 1 cut(s) 476
BspACI CCGC 1 cut(s) 266
BspCNI CTCAG 1 cut(s) 505
BspLI GGNNCC 1 cut(s) 263
BspT107I GGYRCC 1 cut(s) 261
BsrDI GCAATG 2 cut(s) 123, 361
BsrFI RCCGGY 1 cut(s) 332
BssAI RCCGGY 1 cut(s) 332
BssECI CCNNGG 3 cut(s) 274, 457, 476
BssMI GATC 1 cut(s) 247
BssT1I CCWWGG 3 cut(s) 274, 457, 476
Bst4CI ACNGT 1 cut(s) 452
BstC8I GCNNGC 2 cut(s) 334, 394
BstDEI CTNAG 1 cut(s) 513
BstDSI CCRYGG 1 cut(s) 476
BstENI CCTNNNNNAGG 1 cut(s) 273
BstF5I GGATG 1 cut(s) 382
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 1 cut(s) 250
BstMBI GATC 1 cut(s) 247
BstMWI GCNNNNNNNGC 2 cut(s) 402, 518
BstV1I GCAGC 1 cut(s) 352
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BtgI CCRYGG 1 cut(s) 476
BtsCI GGATG 1 cut(s) 382
Cac8I GCNNGC 2 cut(s) 334, 394
CfoI GCGC 1 cut(s) 75
Cfr10I RCCGGY 1 cut(s) 332
Csp6I GTAC 1 cut(s) 16
CviAII CATG 2 cut(s) 25, 477
CviJI RGCY 4 cut(s) 289, 336, 405, 521
CviKI_1 RGCY 4 cut(s) 289, 336, 405, 521
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 1 cut(s) 513
DpnI GATC 1 cut(s) 249
DpnII GATC 1 cut(s) 247
DraI TTTAAA 1 cut(s) 384
Eco130I CCWWGG 3 cut(s) 274, 457, 476
Eco32I GATATC 1 cut(s) 472
EcoNI CCTNNNNNAGG 1 cut(s) 273
EcoRV GATATC 1 cut(s) 472
EcoT14I CCWWGG 3 cut(s) 274, 457, 476
ErhI CCWWGG 3 cut(s) 274, 457, 476
FaeI CATG 2 cut(s) 28, 480
FaiI YATR 9 cut(s) 26, 179, 201, 254, 292, 408, 478, 485, 524
FaqI GGGAC 1 cut(s) 71
FatI CATG 2 cut(s) 24, 476
FauI CCCGC 1 cut(s) 273
Fnu4HI GCNGC 1 cut(s) 366
FokI GGATG 1 cut(s) 389
Fsp4HI GCNGC 1 cut(s) 366
FspAI RTGCGCAY 1 cut(s) 74
FspI TGCGCA 1 cut(s) 74
GlaI GCGC 1 cut(s) 74
GluI GCNGC 1 cut(s) 366
HapII CCGG 1 cut(s) 333
HhaI GCGC 1 cut(s) 75
Hin1II CATG 2 cut(s) 28, 480
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HinfI GANTC 2 cut(s) 431, 515
HpaII CCGG 1 cut(s) 333
HphI GGTGA 2 cut(s) 16, 440
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 2 cut(s) 228, 502
Hpy188III TCNNGA 1 cut(s) 347
Hpy8I GTNNAC 1 cut(s) 208
HpyAV CCTTC 1 cut(s) 157
HpyCH4III ACNGT 1 cut(s) 452
HpyCH4V TGCA 4 cut(s) 31, 128, 199, 354
HpyF10VI GCNNNNNNNGC 2 cut(s) 402, 518
HpyF3I CTNAG 1 cut(s) 513
Hsp92II CATG 2 cut(s) 28, 480
HspAI GCGC 1 cut(s) 73
KroI GCCGGC 1 cut(s) 332
KroNI GCCGGC 1 cut(s) 334
Kzo9I GATC 1 cut(s) 247
LpnPI CCDG 3 cut(s) 169, 258, 346
Lsp1109I GCAGC 1 cut(s) 352
LweI GCATC 2 cut(s) 84, 137
MaeIII GTNAC 2 cut(s) 187, 239
MalI GATC 1 cut(s) 249
MboI GATC 1 cut(s) 247
MboII GAAGA 3 cut(s) 123, 426, 542
MflI RGATCY 1 cut(s) 247
MluCI AATT 1 cut(s) 142
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 1 cut(s) 251
MnlI CCTC 4 cut(s) 94, 241, 279, 418
MroNI GCCGGC 1 cut(s) 332
MseI TTAA 2 cut(s) 383, 447
MspI CCGG 1 cut(s) 333
MwoI GCNNNNNNNGC 2 cut(s) 402, 518
NaeI GCCGGC 1 cut(s) 334
NcoI CCATGG 1 cut(s) 476
NdeII GATC 1 cut(s) 247
NgoMIV GCCGGC 1 cut(s) 332
NlaIII CATG 2 cut(s) 28, 480
NlaIV GGNNCC 1 cut(s) 263
NsbI TGCGCA 1 cut(s) 74
PdiI GCCGGC 1 cut(s) 334
PfeI GAWTC 1 cut(s) 431
PkrI GCNGC 1 cut(s) 367
PleI GAGTC 1 cut(s) 523
PpsI GAGTC 1 cut(s) 523
PsiI TTATAA 1 cut(s) 254
PspN4I GGNNCC 1 cut(s) 263
PsuI RGATCY 1 cut(s) 247
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
SaqAI TTAA 2 cut(s) 383, 447
SatI GCNGC 1 cut(s) 366
Sau3AI GATC 1 cut(s) 247
SchI GAGTC 1 cut(s) 524
SetI ASST 5 cut(s) 168, 233, 280, 291, 325
SfaNI GCATC 2 cut(s) 84, 137
Sse9I AATT 1 cut(s) 142
SsiI CCGC 1 cut(s) 266
SspI AATATT 2 cut(s) 40, 550
StyI CCWWGG 3 cut(s) 274, 457, 476
TaaI ACNGT 1 cut(s) 452
TaqI TCGA 3 cut(s) 99, 297, 423
TasI AATT 1 cut(s) 142
TfiI GAWTC 1 cut(s) 431
Tru1I TTAA 2 cut(s) 383, 447
Tru9I TTAA 2 cut(s) 383, 447
TseI GCWGC 1 cut(s) 365
TspDTI ATGAA 5 cut(s) 26, 50, 136, 372, 528
XagI CCTNNNNNAGG 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.