FvH4_4g04230

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018020
Physical Location & Seq
Forward (+)
3642707 .. 3643681
975 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04230.t1

Sequence Viewer

Length: 762 bp
ATGGAAACCTATACCTCTCCTATCCAGTTGAAGCTCATGATAGACCCGCTGCATCAAAAGGTTTTGTTTGCTGAAGTGGGCAAGGATTTTGTGGATTTTCTATTCACTCTTCTGCCTCTGCCTATTGGCACAATCATTGGCTTACTCAACAAAGAACATGGCATGGTTGGTTGCTTGGGAAAACTCTACAACAGTGTTGAAAGTCTCAGTGGCACATACTTTCTAAACTCTGATCTTGATAGAAATGCTATTGATGATGAATCCAGCCACGATGAATGGGATGATGATGTCAATATAATGGATGTATCAAGCGACAAGCAATCAAGTGATGACAATGAATCAAATGCGAATAGTGACTTCTACATCTGTGTGTCCTGTTTGAGCAAGAGCAGTGCCAAAGAACGGGTATACTATGGCACGGACAACTCTAAATCCATCTGTCCAGGGTGCAAAGCAATAATGTCGACCCCAGCGGCTTATGTTGCTCCACCGCTTACTGAAGATGATATATCCATCCGTGAGGGATCATATGTGAAAGATGCTGTTACTTATATTGTAATGGATGATTTGGAAGTGAAGCCCATGTCTGGCACTTTGGTTATGGCTGTGCTAAACAAGTTTAATGTTAAGGATGTCAGTAGCCTTGAAGAGAAGGTGGTTCATCTTGGCAACGAAGAGGGTCTGAAGTTGCTTAAGGCGTCCCTGCACTCTGAGTCAGCTCTCAGCGTTGCCTTCCTTGCAAGGAAAGAAAAAGCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

254

Amino Acids

27.77

Weight (kDa)

4.6

Isoelectric Point (pI)

31.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 8 - 250 3.2e-38 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018020)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g04230
rosa_chinensis RchiOBHm_Chr4g0394711
rosa_laevigata RLG00000009669
rosa_multiflora Rmu_sc0005356.1_g000017
rosa_roxburghii Rroxscaffold_5G00339730
rosa_rugosa Rorug03G0362300.1 Rorug03G0362400.1
rosa_samantha Rh4AG054600 Rh4CG059100 Rh4DG050800
rosa_wichuraiana Rw4G004350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 408, 464
AciI CCGC 3 cut(s) 47, 473, 491
AclWI GGATC 1 cut(s) 532
AcuI CTGAAG 3 cut(s) 93, 519, 704
AcyI GRCGYC 1 cut(s) 698
AfiI CCNNNNNNNGG 2 cut(s) 402, 587
AflII CTTAAG 1 cut(s) 692
AgsI TTSAA 3 cut(s) 31, 200, 647
AjnI CCWGG 1 cut(s) 442
AluBI AGCT 3 cut(s) 34, 719, 758
AluI AGCT 3 cut(s) 34, 719, 758
Alw26I GTCTC 1 cut(s) 209
AlwI GGATC 1 cut(s) 532
ApeKI GCWGC 2 cut(s) 49, 755
BbvI GCAGC 1 cut(s) 36
BccI CCATC 2 cut(s) 443, 521
BcgI CGANNNNNNTGC 2 cut(s) 444, 478
BciT130I CCWGG 1 cut(s) 444
BcoDI GTCTC 1 cut(s) 209
BfrI CTTAAG 1 cut(s) 692
BisI GCNGC 3 cut(s) 50, 474, 756
BlsI GCNGC 3 cut(s) 51, 475, 757
Bme1390I CCNGG 1 cut(s) 444
BmrFI CCNGG 1 cut(s) 444
BmsI GCATC 2 cut(s) 61, 529
BsaHI GRCGYC 1 cut(s) 698
BsaJI CCNNGG 1 cut(s) 443
Bsc4I CCNNNNNNNGG 2 cut(s) 402, 587
Bse1I ACTGG 1 cut(s) 25
BseBI CCWGG 1 cut(s) 444
BseDI CCNNGG 1 cut(s) 443
BseGI GGATG 5 cut(s) 286, 307, 513, 568, 637
BseLI CCNNNNNNNGG 2 cut(s) 402, 587
BseMII CTCAG 3 cut(s) 220, 702, 736
BseNI ACTGG 1 cut(s) 25
BseXI GCAGC 1 cut(s) 36
BseYI CCCAGC 1 cut(s) 469
BsgI GTGCAG 1 cut(s) 689
BslFI GGGAC 1 cut(s) 685
BslI CCNNNNNNNGG 2 cut(s) 402, 587
BsmAI GTCTC 1 cut(s) 209
BsmFI GGGAC 1 cut(s) 685
Bsp143I GATC 2 cut(s) 232, 524
BspACI CCGC 3 cut(s) 47, 473, 491
BspCNI CTCAG 3 cut(s) 219, 703, 735
BspHI TCATGA 1 cut(s) 36
BspPI GGATC 1 cut(s) 532
BspTI CTTAAG 1 cut(s) 692
BsrI ACTGG 1 cut(s) 25
BssECI CCNNGG 1 cut(s) 443
BssMI GATC 2 cut(s) 232, 524
BssNAI GTATAC 1 cut(s) 409
BssNI GRCGYC 1 cut(s) 698
Bst1107I GTATAC 1 cut(s) 409
Bst2UI CCWGG 1 cut(s) 444
Bst4CI ACNGT 1 cut(s) 194
Bst6I CTCTTC 3 cut(s) 114, 642, 669
BstACI GRCGYC 1 cut(s) 698
BstAFI CTTAAG 1 cut(s) 692
BstDEI CTNAG 3 cut(s) 206, 711, 722
BstF5I GGATG 5 cut(s) 286, 307, 513, 568, 637
BstKTI GATC 2 cut(s) 235, 527
BstMAI GTCTC 1 cut(s) 209
BstMBI GATC 2 cut(s) 232, 524
BstMWI GCNNNNNNNGC 2 cut(s) 482, 737
BstNI CCWGG 1 cut(s) 444
BstSCI CCNGG 1 cut(s) 442
BstV1I GCAGC 1 cut(s) 36
BstZ17I GTATAC 1 cut(s) 409
BtsCI GGATG 5 cut(s) 286, 307, 513, 568, 637
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 3 cut(s) 199, 214, 397
CciI TCATGA 1 cut(s) 36
CseI GACGC 1 cut(s) 687
CviAII CATG 4 cut(s) 37, 158, 163, 583
CviJI RGCY 9 cut(s) 34, 141, 267, 476, 580, 605, 642, 719, 758
CviKI_1 RGCY 9 cut(s) 34, 141, 267, 476, 580, 605, 642, 719, 758
DdeI CTNAG 3 cut(s) 206, 711, 722
DpnI GATC 2 cut(s) 234, 526
DpnII GATC 2 cut(s) 232, 524
Eam1104I CTCTTC 3 cut(s) 114, 642, 669
EarI CTCTTC 3 cut(s) 114, 642, 669
Eco57I CTGAAG 3 cut(s) 93, 519, 704
EcoRII CCWGG 1 cut(s) 442
FaeI CATG 4 cut(s) 40, 161, 166, 586
FaqI GGGAC 1 cut(s) 685
FatI CATG 4 cut(s) 36, 157, 162, 582
FauI CCCGC 1 cut(s) 54
FauNDI CATATG 1 cut(s) 529
FblI GTMKAC 2 cut(s) 408, 464
Fnu4HI GCNGC 3 cut(s) 50, 474, 756
FokI GGATG 5 cut(s) 293, 314, 500, 575, 644
Fsp4HI GCNGC 3 cut(s) 50, 474, 756
GluI GCNGC 3 cut(s) 50, 474, 756
GsaI CCCAGC 1 cut(s) 473
HgaI GACGC 1 cut(s) 687
Hin1I GRCGYC 1 cut(s) 698
Hin1II CATG 4 cut(s) 40, 161, 166, 586
HincII GTYRAC 1 cut(s) 465
HindII GTYRAC 1 cut(s) 465
HinfI GANTC 3 cut(s) 260, 338, 713
Hpy166II GTNNAC 2 cut(s) 409, 465
Hpy188I TCNGA 3 cut(s) 232, 684, 712
Hpy188III TCNNGA 2 cut(s) 37, 236
Hpy8I GTNNAC 2 cut(s) 409, 465
HpyAV CCTTC 2 cut(s) 646, 742
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4V TGCA 4 cut(s) 52, 450, 706, 740
HpyF10VI GCNNNNNNNGC 2 cut(s) 482, 737
HpyF3I CTNAG 3 cut(s) 206, 711, 722
Hsp92I GRCGYC 1 cut(s) 698
Hsp92II CATG 4 cut(s) 40, 161, 166, 586
Kzo9I GATC 2 cut(s) 232, 524
LmnI GCTCC 1 cut(s) 490
LpnPI CCDG 8 cut(s) 38, 277, 388, 429, 456, 483, 573, 716
Lsp1109I GCAGC 1 cut(s) 36
LweI GCATC 2 cut(s) 61, 529
MaeIII GTNAC 2 cut(s) 353, 544
MalI GATC 2 cut(s) 234, 526
MboI GATC 2 cut(s) 232, 524
MboII GAAGA 4 cut(s) 101, 512, 659, 686
MlyI GAGTC 1 cut(s) 722
MnlI CCTC 4 cut(s) 25, 126, 514, 670
MseI TTAA 3 cut(s) 621, 627, 693
MslI CAYNNNNRTG 1 cut(s) 368
MspA1I CMGCKG 2 cut(s) 49, 473
MspCI CTTAAG 1 cut(s) 692
MspR9I CCNGG 1 cut(s) 444
MvaI CCWGG 1 cut(s) 444
MwoI GCNNNNNNNGC 2 cut(s) 482, 737
NdeI CATATG 1 cut(s) 529
NdeII GATC 2 cut(s) 232, 524
NlaIII CATG 4 cut(s) 40, 161, 166, 586
NmuCI GTSAC 1 cut(s) 353
PagI TCATGA 1 cut(s) 36
PfeI GAWTC 2 cut(s) 260, 338
PkrI GCNGC 3 cut(s) 51, 475, 757
PleI GAGTC 1 cut(s) 721
PpsI GAGTC 1 cut(s) 721
Psp6I CCWGG 1 cut(s) 442
PspFI CCCAGC 1 cut(s) 469
PspGI CCWGG 1 cut(s) 442
RseI CAYNNNNRTG 1 cut(s) 368
SalI GTCGAC 1 cut(s) 463
SaqAI TTAA 3 cut(s) 621, 627, 693
SatI GCNGC 3 cut(s) 50, 474, 756
Sau3AI GATC 2 cut(s) 232, 524
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 1 cut(s) 444
SetI ASST 7 cut(s) 11, 17, 36, 63, 657, 721, 760
SfaNI GCATC 2 cut(s) 61, 529
SmiMI CAYNNNNRTG 1 cut(s) 368
SmlI CTYRAG 1 cut(s) 692
SmoI CTYRAG 1 cut(s) 692
SsiI CCGC 3 cut(s) 47, 473, 491
StyD4I CCNGG 1 cut(s) 442
TaaI ACNGT 1 cut(s) 194
TaqI TCGA 1 cut(s) 464
TauI GCSGC 1 cut(s) 476
TfiI GAWTC 2 cut(s) 260, 338
Tru1I TTAA 3 cut(s) 621, 627, 693
Tru9I TTAA 3 cut(s) 621, 627, 693
TscAI CASTG 3 cut(s) 199, 214, 397
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 2 cut(s) 49, 755
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 4 cut(s) 273, 288, 351, 650
TspGWI ACGGA 2 cut(s) 434, 506
TspRI CASTG 3 cut(s) 199, 214, 397
Vha464I CTTAAG 1 cut(s) 692
XmiI GTMKAC 2 cut(s) 408, 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.