Rmu_sc0005356.1_g000017

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005356.1
OG0018020
Physical Location & Seq
Reverse (-)
80735 .. 86434
5700 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005356.1_g000017.1.cds

Sequence Viewer

Length: 1602 bp
atgagctttgcaaaaagcttaccgggttttgtgttgttgcaatcccggttctcctcgccgctagtgggctgctgccggacgggtgttgagcgccagttgctcagaggttcagagggagaagcatcagactcatggcgtcagacggggctggatagtttccagacagagatctccggtgtgttcgcctcgcgttgttgcagggatagggggtccggcgtggagctctgccgattaggacggcagcacggttctgatgggttgaatggcgacacagttctgaggagctgtgcgacggtgggatctagaggatccgagtggcttgggattctggtggggagcgcggcgaggccaagcggtggtcgttgtatgctgggtcgtgccggcgtggctgggcgttctcggcttggtgagttacggcggacagtcaggctggtggtgggagctgacggcattgcacagacacacggggagatacagttttgggcctgcttcctgctgggccatgtggtttttgggcttctcctctttgggctagggcttttgctcttggcccaactttatgtttttgtaaagtacttggtccaaattgctaatacgaaaactactgagataatagttggtctgaagagtcgactcgactcggacacgcttctacagcggactgtgggtctcatcgtctccctccctgagaacacacccctctgtcctcactcacaccattctttctccatttcaagttggggtttcctccattaccaggtagtctgcagattgatcagactcatttcatccatggagacctacacctctcctatcaagttgaagctcatgatagacccgctgtgtcaaaaggttttgtttgctgaagtgggcaaggattttgtggattttctattcactcttctgcctttgcctattggcacaatcattggcttactcaaagaagaacatggaatggttggttgcttaggaaaactctacaacagtgttgaaaatctcagtagcacatacttgctaaaccctgatcttgacagagatgtactgctaaatcctatgtcaccagttgatactgctgatattttgaacttgttgacccaagaggaaggtactgatgatgaatccagtcacgatgaatggaatgatgacaatgaatcaagcgacaagcaatcaagtgatgacaatgaatcaaatgctaatagtgacttctatatatgtgtgtcctgtttgggcaagagcaatgccaaagaacgggtatactatgtcacggataactctaaatccatttgtccagggtgcaaagcaataatgtcgaccccagcgacttatgttgctccaccgcttactgaggatgaagtatcattccgcgagggatcgtatgtgaaagatgttgttacttatattgtaatggatgatttggaagtgaggcccatgtctgccactttgattatggctatgttaaaaaaattcaatgttaaggatgtcagtagccttatagagaaggtggttcctcttggcaacgaagagggtctgaagttgcttaaggcgtccctgcagtcagagtcagctctcagcactgccttccttgtagggaaagaagcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

533

Amino Acids

58.18

Weight (kDa)

5.44

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018020)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g04230
rosa_chinensis RchiOBHm_Chr4g0394711
rosa_laevigata RLG00000009669
rosa_multiflora Rmu_sc0005356.1_g000017
rosa_roxburghii Rroxscaffold_5G00339730
rosa_rugosa Rorug03G0362300.1 Rorug03G0362400.1
rosa_samantha Rh4AG054600 Rh4CG059100 Rh4DG050800
rosa_wichuraiana Rw4G004350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 356
AccI GTMKAC 3 cut(s) 631, 1254, 1310
AccII CGCG 3 cut(s) 190, 341, 1365
AciI CCGC 8 cut(s) 59, 341, 354, 418, 658, 839, 1337, 1363
AclWI GGATC 4 cut(s) 303, 307, 316, 1378
AcsI RAATTY 1 cut(s) 1463
AcuI CTGAAG 3 cut(s) 644, 885, 1550
AcyI GRCGYC 2 cut(s) 136, 1544
AfaI GTAC 3 cut(s) 575, 1041, 1108
AfiI CCNNNNNNNGG 4 cut(s) 65, 356, 757, 1248
AflII CTTAAG 1 cut(s) 1538
AgsI TTSAA 6 cut(s) 262, 735, 823, 992, 1084, 1468
AhdI GACNNNNNGTC 1 cut(s) 666
AjnI CCWGG 2 cut(s) 756, 1288
AluBI AGCT 7 cut(s) 6, 18, 223, 285, 443, 826, 1565
AluI AGCT 7 cut(s) 6, 18, 223, 285, 443, 826, 1565
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 3 cut(s) 674, 682, 791
AlwI GGATC 4 cut(s) 303, 307, 316, 1378
AoxI GGCC 5 cut(s) 347, 483, 499, 549, 1424
ApeKI GCWGC 3 cut(s) 69, 72, 241
ApoI RAATTY 1 cut(s) 1463
AspLEI GCGC 2 cut(s) 93, 341
AspS9I GGNCC 6 cut(s) 210, 483, 499, 550, 580, 1425
AsuC2I CCSGG 2 cut(s) 24, 46
AsuHPI GGTGA 2 cut(s) 419, 1050
AvaII GGWCC 2 cut(s) 210, 580
BamHI GGATCC 1 cut(s) 308
BanII GRGCYC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 3 cut(s) 56, 59, 253
BccI CCATC 1 cut(s) 248
BceAI ACGGC 3 cut(s) 254, 431, 463
BcgI CGANNNNNNTGC 2 cut(s) 1290, 1324
BciT130I CCWGG 2 cut(s) 758, 1290
BclI TGATCA 1 cut(s) 774
BcnI CCSGG 2 cut(s) 24, 46
BcoDI GTCTC 3 cut(s) 674, 682, 791
BfaI CTAG 3 cut(s) 62, 303, 533
BfmI CTRYAG 3 cut(s) 653, 766, 1550
BfoI RGCGCY 1 cut(s) 94
BfrI CTTAAG 1 cut(s) 1538
BglI GCCNNNNNGGC 1 cut(s) 386
BglII AGATCT 1 cut(s) 168
BisI GCNGC 5 cut(s) 59, 70, 73, 242, 342
BlsI GCNGC 5 cut(s) 60, 71, 74, 243, 343
BmcAI AGTACT 1 cut(s) 575
Bme1390I CCNGG 4 cut(s) 24, 46, 758, 1290
Bme18I GGWCC 2 cut(s) 210, 580
BmeRI GACNNNNNGTC 1 cut(s) 666
BmgT120I GGNCC 6 cut(s) 210, 483, 499, 550, 580, 1425
BmiI GGNNCC 3 cut(s) 211, 310, 1506
BmrFI CCNGG 4 cut(s) 24, 46, 758, 1290
BmsI GCATC 1 cut(s) 131
Bpu10I CCTNAGC 1 cut(s) 967
BpuMI CCSGG 2 cut(s) 24, 46
BsaHI GRCGYC 2 cut(s) 136, 1544
BsaI GGTCTC 2 cut(s) 674, 791
BsaJI CCNNGG 2 cut(s) 792, 1289
BsaWI WCCGGW 1 cut(s) 173
BsaXI ACNNNNNCTCC 2 cut(s) 461, 491
Bsc4I CCNNNNNNNGG 4 cut(s) 65, 356, 757, 1248
Bse118I RCCGGY 1 cut(s) 380
Bse1I ACTGG 3 cut(s) 94, 1061, 1122
Bse3DI GCAATG 2 cut(s) 450, 1243
BseBI CCWGG 2 cut(s) 758, 1290
BseDI CCNNGG 2 cut(s) 792, 1289
BseGI GGATG 4 cut(s) 788, 1354, 1414, 1483
BseLI CCNNNNNNNGG 4 cut(s) 65, 356, 757, 1248
BseMI GCAATG 2 cut(s) 450, 1243
BseMII CTCAG 7 cut(s) 115, 269, 597, 678, 1012, 1335, 1582
BseNI ACTGG 3 cut(s) 94, 1061, 1122
BseRI GAGGAG 3 cut(s) 43, 295, 512
BseXI GCAGC 3 cut(s) 56, 59, 253
BseYI CCCAGC 4 cut(s) 370, 389, 496, 1315
Bsh1236I CGCG 3 cut(s) 190, 341, 1365
BshFI GGCC 5 cut(s) 349, 485, 501, 551, 1426
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 6 cut(s) 23, 46, 76, 174, 213, 381
BslFI GGGAC 1 cut(s) 1531
BslI CCNNNNNNNGG 4 cut(s) 65, 356, 757, 1248
BsmAI GTCTC 3 cut(s) 674, 682, 791
BsmBI CGTCTC 1 cut(s) 682
BsmFI GGGAC 1 cut(s) 1531
BsnI GGCC 5 cut(s) 349, 485, 501, 551, 1426
Bso31I GGTCTC 2 cut(s) 674, 791
Bsp1286I GDGCHC 1 cut(s) 225
Bsp143I GATC 6 cut(s) 168, 299, 308, 774, 1024, 1370
Bsp19I CCATGG 1 cut(s) 792
BspACI CCGC 8 cut(s) 59, 341, 354, 418, 658, 839, 1337, 1363
BspANI GGCC 5 cut(s) 349, 485, 501, 551, 1426
BspCNI CTCAG 7 cut(s) 114, 270, 598, 679, 1011, 1336, 1581
BspFNI CGCG 3 cut(s) 190, 341, 1365
BspHI TCATGA 1 cut(s) 828
BspLI GGNNCC 3 cut(s) 211, 310, 1506
BspMAI CTGCAG 2 cut(s) 770, 1554
BspPI GGATC 4 cut(s) 303, 307, 316, 1378
BspTI CTTAAG 1 cut(s) 1538
BspTNI GGTCTC 2 cut(s) 674, 791
BsrDI GCAATG 2 cut(s) 450, 1243
BsrFI RCCGGY 1 cut(s) 380
BsrI ACTGG 3 cut(s) 94, 1061, 1122
BssAI RCCGGY 1 cut(s) 380
BssECI CCNNGG 2 cut(s) 792, 1289
BssMI GATC 6 cut(s) 168, 299, 308, 774, 1024, 1370
BssNAI GTATAC 1 cut(s) 1255
BssNI GRCGYC 2 cut(s) 136, 1544
BssT1I CCWWGG 1 cut(s) 792
Bst1107I GTATAC 1 cut(s) 1255
Bst2UI CCWGG 2 cut(s) 758, 1290
Bst4CI ACNGT 7 cut(s) 248, 274, 295, 424, 477, 664, 986
Bst6I CTCTTC 3 cut(s) 620, 906, 1515
BstACI GRCGYC 2 cut(s) 136, 1544
BstAFI CTTAAG 1 cut(s) 1538
BstC8I GCNNGC 2 cut(s) 382, 487
BstDEI CTNAG 8 cut(s) 101, 278, 606, 687, 967, 998, 1344, 1568
BstDSI CCRYGG 1 cut(s) 792
BstF5I GGATG 4 cut(s) 788, 1354, 1414, 1483
BstFNI CGCG 3 cut(s) 190, 341, 1365
BstH2I RGCGCY 1 cut(s) 94
BstHHI GCGC 2 cut(s) 93, 341
BstKTI GATC 6 cut(s) 171, 302, 311, 777, 1027, 1373
BstMAI GTCTC 3 cut(s) 674, 682, 791
BstMBI GATC 6 cut(s) 168, 299, 308, 774, 1024, 1370
BstMWI GCNNNNNNNGC 4 cut(s) 97, 386, 400, 655
BstNI CCWGG 2 cut(s) 758, 1290
BstSCI CCNGG 4 cut(s) 22, 44, 756, 1288
BstSFI CTRYAG 3 cut(s) 653, 766, 1550
BstUI CGCG 3 cut(s) 190, 341, 1365
BstV1I GCAGC 3 cut(s) 56, 59, 253
BstX2I RGATCY 3 cut(s) 168, 299, 308
BstYI RGATCY 3 cut(s) 168, 299, 308
BstZ17I GTATAC 1 cut(s) 1255
BsuRI GGCC 5 cut(s) 349, 485, 501, 551, 1426
BtgI CCRYGG 1 cut(s) 792
BtsCI GGATG 4 cut(s) 788, 1354, 1414, 1483
BtsI GCAGTG 1 cut(s) 1572
BtsIMutI CAGTG 2 cut(s) 991, 1572
Cac8I GCNNGC 2 cut(s) 382, 487
CciI TCATGA 1 cut(s) 828
CfoI GCGC 2 cut(s) 93, 341
Cfr10I RCCGGY 1 cut(s) 380
Cfr13I GGNCC 6 cut(s) 210, 483, 499, 550, 580, 1425
CseI GACGC 2 cut(s) 125, 1533
CsiI ACCWGGT 1 cut(s) 756
Csp6I GTAC 3 cut(s) 574, 1040, 1107
CviAII CATG 7 cut(s) 132, 503, 793, 829, 950, 1429, 1599
CviQI GTAC 3 cut(s) 574, 1040, 1107
DdeI CTNAG 8 cut(s) 101, 278, 606, 687, 967, 998, 1344, 1568
DpnI GATC 6 cut(s) 170, 301, 310, 776, 1026, 1372
DpnII GATC 6 cut(s) 168, 299, 308, 774, 1024, 1370
DriI GACNNNNNGTC 1 cut(s) 666
Eam1104I CTCTTC 3 cut(s) 620, 906, 1515
Eam1105I GACNNNNNGTC 1 cut(s) 666
EarI CTCTTC 3 cut(s) 620, 906, 1515
EciI GGCGGA 1 cut(s) 433
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 792
Eco24I GRGCYC 1 cut(s) 225
Eco31I GGTCTC 2 cut(s) 674, 791
Eco47I GGWCC 2 cut(s) 210, 580
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 3 cut(s) 644, 885, 1550
EcoICRI GAGCTC 1 cut(s) 223
EcoRII CCWGG 2 cut(s) 756, 1288
EcoT14I CCWWGG 1 cut(s) 792
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 792
Esp3I CGTCTC 1 cut(s) 682
FaeI CATG 7 cut(s) 135, 506, 796, 832, 953, 1432, 1602
FaqI GGGAC 1 cut(s) 1531
FatI CATG 7 cut(s) 131, 502, 792, 828, 949, 1428, 1598
FauI CCCGC 1 cut(s) 846
FbaI TGATCA 1 cut(s) 774
FblI GTMKAC 3 cut(s) 631, 1254, 1310
Fnu4HI GCNGC 5 cut(s) 59, 70, 73, 242, 342
FokI GGATG 4 cut(s) 775, 1361, 1421, 1490
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 5 cut(s) 59, 70, 73, 242, 342
FspBI CTAG 3 cut(s) 62, 303, 533
GlaI GCGC 2 cut(s) 92, 340
GluI GCNGC 5 cut(s) 59, 70, 73, 242, 342
GsaI CCCAGC 4 cut(s) 374, 393, 500, 1319
HaeII RGCGCY 1 cut(s) 94
HaeIII GGCC 5 cut(s) 349, 485, 501, 551, 1426
HapII CCGG 6 cut(s) 23, 46, 76, 174, 213, 381
HgaI GACGC 2 cut(s) 125, 1533
HhaI GCGC 2 cut(s) 93, 341
Hin1I GRCGYC 2 cut(s) 136, 1544
Hin1II CATG 7 cut(s) 135, 506, 796, 832, 953, 1432, 1602
Hin6I GCGC 2 cut(s) 91, 339
HinP1I GCGC 2 cut(s) 91, 339
HincII GTYRAC 3 cut(s) 632, 1092, 1311
HindII GTYRAC 3 cut(s) 632, 1092, 1311
HindIII AAGCTT 1 cut(s) 16
HpaII CCGG 6 cut(s) 23, 46, 76, 174, 213, 381
HphI GGTGA 2 cut(s) 419, 1050
Hpy166II GTNNAC 4 cut(s) 632, 1092, 1255, 1311
Hpy188III TCNNGA 5 cut(s) 160, 303, 829, 1028, 1127
Hpy8I GTNNAC 4 cut(s) 632, 1092, 1255, 1311
Hpy99I CGWCG 1 cut(s) 295
HpyAV CCTTC 3 cut(s) 1097, 1492, 1588
HpyCH4III ACNGT 7 cut(s) 248, 274, 295, 424, 477, 664, 986
HpyCH4V TGCA 7 cut(s) 11, 40, 198, 455, 768, 1296, 1552
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 386, 400, 655
HpyF3I CTNAG 8 cut(s) 101, 278, 606, 687, 967, 998, 1344, 1568
Hsp92I GRCGYC 2 cut(s) 136, 1544
Hsp92II CATG 7 cut(s) 135, 506, 796, 832, 953, 1432, 1602
HspAI GCGC 2 cut(s) 91, 339
KroI GCCGGC 1 cut(s) 380
KroNI GCCGGC 1 cut(s) 382
Ksp22I TGATCA 1 cut(s) 774
Kzo9I GATC 6 cut(s) 168, 299, 308, 774, 1024, 1370
LmnI GCTCC 5 cut(s) 220, 282, 336, 440, 1336
Lsp1109I GCAGC 3 cut(s) 56, 59, 253
LweI GCATC 1 cut(s) 131
MabI ACCWGGT 1 cut(s) 756
MaeI CTAG 3 cut(s) 62, 303, 533
MaeIII GTNAC 6 cut(s) 411, 1056, 1124, 1199, 1261, 1390
MalI GATC 6 cut(s) 170, 301, 310, 776, 1026, 1372
MboI GATC 6 cut(s) 168, 299, 308, 774, 1024, 1370
MboII GAAGA 4 cut(s) 637, 893, 956, 1532
MflI RGATCY 3 cut(s) 168, 299, 308
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 2 cut(s) 585, 1463
MlyI GAGTC 6 cut(s) 122, 627, 632, 637, 774, 1568
MroNI GCCGGC 1 cut(s) 380
MseI TTAA 3 cut(s) 1457, 1473, 1539
MspA1I CMGCKG 2 cut(s) 658, 841
MspCI CTTAAG 1 cut(s) 1538
MspI CCGG 6 cut(s) 23, 46, 76, 174, 213, 381
MspR9I CCNGG 4 cut(s) 24, 46, 758, 1290
MvaI CCWGG 2 cut(s) 758, 1290
MvnI CGCG 3 cut(s) 190, 341, 1365
MwoI GCNNNNNNNGC 4 cut(s) 97, 386, 400, 655
NaeI GCCGGC 1 cut(s) 382
NciI CCSGG 2 cut(s) 24, 46
NcoI CCATGG 1 cut(s) 792
NdeII GATC 6 cut(s) 168, 299, 308, 774, 1024, 1370
NgoMIV GCCGGC 1 cut(s) 380
NlaIII CATG 7 cut(s) 135, 506, 796, 832, 953, 1432, 1602
NlaIV GGNNCC 3 cut(s) 211, 310, 1506
NmeAIII GCCGAG 1 cut(s) 379
NmuCI GTSAC 4 cut(s) 1056, 1124, 1199, 1261
PagI TCATGA 1 cut(s) 828
PcsI WCGNNNNNNNCGW 1 cut(s) 1316
PdiI GCCGGC 1 cut(s) 382
PfeI GAWTC 4 cut(s) 325, 1118, 1151, 1184
PflMI CCANNNNNTGG 1 cut(s) 356
PkrI GCNGC 5 cut(s) 60, 71, 74, 243, 343
PleI GAGTC 6 cut(s) 122, 627, 632, 636, 774, 1567
PpsI GAGTC 6 cut(s) 122, 627, 632, 636, 774, 1567
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 2 cut(s) 756, 1288
PspFI CCCAGC 4 cut(s) 370, 389, 496, 1315
PspGI CCWGG 2 cut(s) 756, 1288
PspN4I GGNNCC 3 cut(s) 211, 310, 1506
PspPI GGNCC 6 cut(s) 210, 483, 499, 550, 580, 1425
PstI CTGCAG 2 cut(s) 770, 1554
PsuI RGATCY 3 cut(s) 168, 299, 308
RsaI GTAC 3 cut(s) 575, 1041, 1108
RsaNI GTAC 3 cut(s) 574, 1040, 1107
SacI GAGCTC 1 cut(s) 225
SalI GTCGAC 2 cut(s) 630, 1309
SaqAI TTAA 3 cut(s) 1457, 1473, 1539
SatI GCNGC 5 cut(s) 59, 70, 73, 242, 342
Sau3AI GATC 6 cut(s) 168, 299, 308, 774, 1024, 1370
Sau96I GGNCC 6 cut(s) 210, 483, 499, 550, 580, 1425
ScaI AGTACT 1 cut(s) 575
SchI GAGTC 6 cut(s) 122, 627, 632, 637, 774, 1568
ScrFI CCNGG 4 cut(s) 24, 46, 758, 1290
SduI GDGCHC 1 cut(s) 225
SexAI ACCWGGT 1 cut(s) 756
SfaNI GCATC 1 cut(s) 131
SfcI CTRYAG 3 cut(s) 653, 766, 1550
SinI GGWCC 2 cut(s) 210, 580
SmlI CTYRAG 1 cut(s) 1538
SmoI CTYRAG 1 cut(s) 1538
Sse9I AATT 2 cut(s) 585, 1463
SsiI CCGC 8 cut(s) 59, 341, 354, 418, 658, 839, 1337, 1363
SspMI CTAG 3 cut(s) 62, 303, 533
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 4 cut(s) 22, 44, 756, 1288
StyI CCWWGG 1 cut(s) 792
TaaI ACNGT 7 cut(s) 248, 274, 295, 424, 477, 664, 986
TaqI TCGA 3 cut(s) 631, 636, 1310
TasI AATT 2 cut(s) 585, 1463
TatI WGTACW 2 cut(s) 573, 1039
TauI GCSGC 2 cut(s) 61, 344
TfiI GAWTC 4 cut(s) 325, 1118, 1151, 1184
Tru1I TTAA 3 cut(s) 1457, 1473, 1539
Tru9I TTAA 3 cut(s) 1457, 1473, 1539
TscAI CASTG 2 cut(s) 991, 1579
TseFI GTSAC 4 cut(s) 1056, 1124, 1199, 1261
TseI GCWGC 3 cut(s) 69, 72, 241
Tsp45I GTSAC 4 cut(s) 1056, 1124, 1199, 1261
TspDTI ATGAA 6 cut(s) 777, 1131, 1146, 1164, 1197, 1365
TspGWI ACGGA 1 cut(s) 1280
TspRI CASTG 2 cut(s) 991, 1579
Van91I CCANNNNNTGG 1 cut(s) 356
Vha464I CTTAAG 1 cut(s) 1538
VpaK11BI GGWCC 2 cut(s) 210, 580
XapI RAATTY 1 cut(s) 1463
XbaI TCTAGA 1 cut(s) 302
XcmI CCANNNNNNNNNTGG 2 cut(s) 509, 1444
XmiI GTMKAC 3 cut(s) 631, 1254, 1310
XspI CTAG 3 cut(s) 62, 303, 533
ZrmI AGTACT 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.