RLG00000009669

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0018020
Physical Location & Seq
Reverse (-)
56226878 .. 56228432
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009669

Sequence Viewer

Length: 645 bp
ATGAACTCTAGCTTTATTTTATTTTTCTTCGAGAAAACCCTTTACGAACTAGGCATGCCCTACTATGGTTTGTTTCAAGTAGTCTGCATATCGATCAGACTCGTTACATCCATGGAAACCTACACCTCTCCTATCAAGTTGAAACTCATGATAGACCCGCTGCGTCAAAAGGGTTTATTTGCTGAAGTGGGCAAGGATTTTGTGGATTTTCTATTCACTCTTCTGCCTTTGCCTATTGGCACAATCATTGGCTTACTCAAAGAAGAACATGGAATGGTTGGTTGCTTAGGAAAACTCTACAACAGTGTTGAAAATCTCAGTAGCACATACTTGCTAAACCCTGATCTTGACAGAGATGTACTGCTAAACCCTATGTCACCAGTTGATACTGCTGATGTTTTAAACTTGTTGACCCAAGAGGAAGGTACTGATGATGAATCCAGTCACGATGAATGGAATAATGACAATGAATCAAGCGACAAGCAATCAAGTGATGACAATAGTGACTTCTATATATGTGTGTCCTGTTTGAGCAAGAGCAATGCCAAAGAACGGGGTGCAAAGCAATATTGTCGACCCCAGCAACTTATGTTGCTCCACCGCTTACGGAGGATGAAATATCATTCCGCGAGGGATCGTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

215

Amino Acids

24.5

Weight (kDa)

5.02

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 45 - 129 1.2e-19 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018020)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g04230
rosa_chinensis RchiOBHm_Chr4g0394711
rosa_laevigata RLG00000009669
rosa_multiflora Rmu_sc0005356.1_g000017
rosa_roxburghii Rroxscaffold_5G00339730
rosa_rugosa Rorug03G0362300.1 Rorug03G0362400.1
rosa_samantha Rh4AG054600 Rh4CG059100 Rh4DG050800
rosa_wichuraiana Rw4G004350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 574
AccII CGCG 1 cut(s) 629
AciI CCGC 3 cut(s) 158, 601, 627
AclWI GGATC 1 cut(s) 642
AcuI CTGAAG 1 cut(s) 204
AfaI GTAC 2 cut(s) 360, 427
AfiI CCNNNNNNNGG 2 cut(s) 65, 552
AgsI TTSAA 3 cut(s) 77, 142, 311
AluBI AGCT 1 cut(s) 12
AluI AGCT 1 cut(s) 12
AlwI GGATC 1 cut(s) 642
ApeKI GCWGC 1 cut(s) 160
AsuHPI GGTGA 1 cut(s) 369
BbvI GCAGC 1 cut(s) 147
BcgI CGANNNNNNTGC 2 cut(s) 554, 588
BfaI CTAG 2 cut(s) 9, 50
BisI GCNGC 1 cut(s) 161
BlsI GCNGC 1 cut(s) 162
Bpu10I CCTNAGC 1 cut(s) 286
Bsa29I ATCGAT 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 111
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 552
Bse1I ACTGG 2 cut(s) 380, 441
Bse3DI GCAATG 1 cut(s) 547
BseCI ATCGAT 1 cut(s) 92
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 2 cut(s) 107, 618
BseLI CCNNNNNNNGG 2 cut(s) 65, 552
BseMI GCAATG 1 cut(s) 547
BseMII CTCAG 1 cut(s) 331
BseNI ACTGG 2 cut(s) 380, 441
BseXI GCAGC 1 cut(s) 147
BseYI CCCAGC 1 cut(s) 579
Bsh1236I CGCG 1 cut(s) 629
BshVI ATCGAT 1 cut(s) 92
BslI CCNNNNNNNGG 2 cut(s) 65, 552
Bsp143I GATC 3 cut(s) 93, 343, 634
Bsp19I CCATGG 1 cut(s) 111
BspACI CCGC 3 cut(s) 158, 601, 627
BspCNI CTCAG 1 cut(s) 330
BspDI ATCGAT 1 cut(s) 92
BspFNI CGCG 1 cut(s) 629
BspHI TCATGA 1 cut(s) 147
BspPI GGATC 1 cut(s) 642
BsrDI GCAATG 1 cut(s) 547
BsrI ACTGG 2 cut(s) 380, 441
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 3 cut(s) 93, 343, 634
BssT1I CCWWGG 1 cut(s) 111
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 1 cut(s) 225
BstC8I GCNNGC 1 cut(s) 56
BstDEI CTNAG 2 cut(s) 286, 317
BstDSI CCRYGG 1 cut(s) 111
BstF5I GGATG 2 cut(s) 107, 618
BstFNI CGCG 1 cut(s) 629
BstKTI GATC 3 cut(s) 96, 346, 637
BstMBI GATC 3 cut(s) 93, 343, 634
BstNSI RCATGY 1 cut(s) 58
BstUI CGCG 1 cut(s) 629
BstV1I GCAGC 1 cut(s) 147
Bsu15I ATCGAT 1 cut(s) 92
BsuTUI ATCGAT 1 cut(s) 92
BtgI CCRYGG 1 cut(s) 111
BtsCI GGATG 2 cut(s) 107, 618
BtsIMutI CAGTG 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 56
CciI TCATGA 1 cut(s) 147
ClaI ATCGAT 1 cut(s) 92
CseI GACGC 1 cut(s) 152
Csp6I GTAC 2 cut(s) 359, 426
CviAII CATG 4 cut(s) 55, 112, 148, 269
CviJI RGCY 2 cut(s) 12, 252
CviKI_1 RGCY 2 cut(s) 12, 252
CviQI GTAC 2 cut(s) 359, 426
DdeI CTNAG 2 cut(s) 286, 317
DpnI GATC 3 cut(s) 95, 345, 636
DpnII GATC 3 cut(s) 93, 343, 634
DraI TTTAAA 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Eco130I CCWWGG 1 cut(s) 111
Eco57I CTGAAG 1 cut(s) 204
EcoT14I CCWWGG 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 4 cut(s) 58, 115, 151, 272
FatI CATG 4 cut(s) 54, 111, 147, 268
FauI CCCGC 1 cut(s) 165
FblI GTMKAC 1 cut(s) 574
Fnu4HI GCNGC 1 cut(s) 161
FokI GGATG 2 cut(s) 94, 625
Fsp4HI GCNGC 1 cut(s) 161
FspBI CTAG 2 cut(s) 9, 50
GluI GCNGC 1 cut(s) 161
GsaI CCCAGC 1 cut(s) 583
HgaI GACGC 1 cut(s) 152
Hin1II CATG 4 cut(s) 58, 115, 151, 272
HincII GTYRAC 2 cut(s) 411, 575
HindII GTYRAC 2 cut(s) 411, 575
HinfI GANTC 3 cut(s) 99, 437, 470
HphI GGTGA 1 cut(s) 369
Hpy166II GTNNAC 2 cut(s) 411, 575
Hpy188I TCNGA 1 cut(s) 98
Hpy188III TCNNGA 4 cut(s) 31, 148, 347, 446
Hpy8I GTNNAC 2 cut(s) 411, 575
HpyAV CCTTC 1 cut(s) 416
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4V TGCA 2 cut(s) 87, 560
HpyF3I CTNAG 2 cut(s) 286, 317
Hsp92II CATG 4 cut(s) 58, 115, 151, 272
Kzo9I GATC 3 cut(s) 93, 343, 634
LmnI GCTCC 1 cut(s) 600
LpnPI CCDG 5 cut(s) 354, 393, 454, 538, 593
Lsp1109I GCAGC 1 cut(s) 147
MaeI CTAG 2 cut(s) 9, 50
MaeIII GTNAC 4 cut(s) 103, 375, 443, 503
MalI GATC 3 cut(s) 95, 345, 636
MboI GATC 3 cut(s) 93, 343, 634
MboII GAAGA 3 cut(s) 19, 212, 275
MlyI GAGTC 1 cut(s) 93
MnlI CCTC 4 cut(s) 136, 412, 603, 624
MseI TTAA 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 160
MvnI CGCG 1 cut(s) 629
NcoI CCATGG 1 cut(s) 111
NdeII GATC 3 cut(s) 93, 343, 634
NlaIII CATG 4 cut(s) 58, 115, 151, 272
NmuCI GTSAC 3 cut(s) 375, 443, 503
NspI RCATGY 1 cut(s) 58
PaeI GCATGC 1 cut(s) 58
PagI TCATGA 1 cut(s) 147
PfeI GAWTC 2 cut(s) 437, 470
PkrI GCNGC 1 cut(s) 162
PleI GAGTC 1 cut(s) 93
PpsI GAGTC 1 cut(s) 93
PspFI CCCAGC 1 cut(s) 579
RsaI GTAC 2 cut(s) 360, 427
RsaNI GTAC 2 cut(s) 359, 426
SalI GTCGAC 1 cut(s) 573
SaqAI TTAA 1 cut(s) 401
SatI GCNGC 1 cut(s) 161
Sau3AI GATC 3 cut(s) 93, 343, 634
SchI GAGTC 1 cut(s) 93
SetI ASST 4 cut(s) 14, 122, 128, 427
SphI GCATGC 1 cut(s) 58
SsiI CCGC 3 cut(s) 158, 601, 627
SspI AATATT 1 cut(s) 569
SspMI CTAG 2 cut(s) 9, 50
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 1 cut(s) 305
TaqI TCGA 3 cut(s) 30, 92, 574
TatI WGTACW 1 cut(s) 358
TfiI GAWTC 2 cut(s) 437, 470
Tru1I TTAA 1 cut(s) 401
Tru9I TTAA 1 cut(s) 401
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 3 cut(s) 375, 443, 503
TseI GCWGC 1 cut(s) 160
Tsp45I GTSAC 3 cut(s) 375, 443, 503
TspDTI ATGAA 5 cut(s) 17, 450, 465, 483, 629
TspGWI ACGGA 1 cut(s) 622
TspRI CASTG 1 cut(s) 310
XceI RCATGY 1 cut(s) 58
XmiI GTMKAC 1 cut(s) 574
XspI CTAG 2 cut(s) 9, 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.