FvH4_4g04870

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018414
Physical Location & Seq
Forward (+)
4233306 .. 4234443
1138 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04870.t1

Sequence Viewer

Length: 372 bp
ATGGCTAGTTTTACTGCTACGTTTTCGTATCTGAGGGTCGCATGCGATGGCCTTGGATGCAATGCCTTGATCCAGGGACGGTATTTTACTTGCCTCGACTGCTCCATTCCCGGCGGACCTAACAGTTTCGATCTCTGCATCTCCTGCCATTTGAACCGAAACTACTCTCACCTCCACACCACTTTTGTGGAAAAGCACGTCTCTGCGTTTCCGGCTGCAAATTACCGACTAGCAGCGTTTGCAACGGTGCCCCAACCGGCGGCGAAACTGAATTACAACAATGAGGGAGTAGCAACGAAGGGAGTAGAAGCAGTCAATACCGGTTCTACCATTCCACATGTAACAGATGATGTCTGCTGCAGCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

124

Amino Acids

13.2

Weight (kDa)

6.48

Isoelectric Point (pI)

32.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018414)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g04870
rosa_chinensis RchiOBHm_Chr4g0395651 RchiOBHm_Chr4g0395681
rosa_roxburghii Rroxscaffold_5G00340560
rosa_rugosa Rorug03G0355000
rosa_samantha Rh4BG061000 Rh4CG067000 Rh4CG067100 Rh4DG056400
rosa_wichuraiana Rw4G004960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 247
AciI CCGC 2 cut(s) 114, 260
AclWI GGATC 1 cut(s) 64
AfiI CCNNNNNNNGG 1 cut(s) 259
AflIII ACRYGT 1 cut(s) 337
AgeI ACCGGT 1 cut(s) 320
AgsI TTSAA 1 cut(s) 154
AjiI CACGTC 1 cut(s) 199
AjnI CCWGG 1 cut(s) 72
AleI CACNNNNGTG 1 cut(s) 185
Alw26I GTCTC 1 cut(s) 205
AlwI GGATC 1 cut(s) 64
AoxI GGCC 1 cut(s) 49
ApeKI GCWGC 4 cut(s) 215, 233, 357, 360
AsiGI ACCGGT 1 cut(s) 320
AspS9I GGNCC 1 cut(s) 116
AsuC2I CCSGG 1 cut(s) 111
AsuHPI GGTGA 1 cut(s) 161
AvaII GGWCC 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 252
BanI GGYRCC 1 cut(s) 247
BbvI GCAGC 3 cut(s) 202, 245, 344
BccI CCATC 1 cut(s) 41
BciT130I CCWGG 1 cut(s) 74
BcnI CCSGG 1 cut(s) 111
BcoDI GTCTC 1 cut(s) 205
BfaI CTAG 2 cut(s) 6, 230
BfmI CTRYAG 1 cut(s) 358
BisI GCNGC 5 cut(s) 216, 234, 261, 358, 361
BlsI GCNGC 5 cut(s) 217, 235, 262, 359, 362
Bme1390I CCNGG 2 cut(s) 74, 111
Bme18I GGWCC 1 cut(s) 116
BmgBI CACGTC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 2 cut(s) 74, 111
BmsI GCATC 2 cut(s) 47, 147
BpuMI CCSGG 1 cut(s) 111
BsaJI CCNNGG 2 cut(s) 52, 73
BsaWI WCCGGW 1 cut(s) 320
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse118I RCCGGY 2 cut(s) 256, 320
Bse3DI GCAATG 1 cut(s) 67
BseBI CCWGG 1 cut(s) 74
BseDI CCNNGG 2 cut(s) 52, 73
BseGI GGATG 1 cut(s) 62
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMI GCAATG 1 cut(s) 67
BseMII CTCAG 1 cut(s) 23
BseSI GKGCMC 1 cut(s) 252
BseXI GCAGC 3 cut(s) 202, 245, 344
BshFI GGCC 1 cut(s) 51
BshNI GGYRCC 1 cut(s) 247
BshTI ACCGGT 1 cut(s) 320
BsiSI CCGG 4 cut(s) 111, 212, 257, 321
BslFI GGGAC 1 cut(s) 90
BslI CCNNNNNNNGG 1 cut(s) 259
BsmAI GTCTC 1 cut(s) 205
BsmBI CGTCTC 1 cut(s) 205
BsmFI GGGAC 1 cut(s) 90
BsnI GGCC 1 cut(s) 51
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 2 cut(s) 69, 130
BspACI CCGC 2 cut(s) 114, 260
BspANI GGCC 1 cut(s) 51
BspCNI CTCAG 1 cut(s) 24
BspLI GGNNCC 1 cut(s) 249
BspMAI CTGCAG 1 cut(s) 362
BspPI GGATC 1 cut(s) 64
BspT107I GGYRCC 1 cut(s) 247
BsrDI GCAATG 1 cut(s) 67
BsrFI RCCGGY 2 cut(s) 256, 320
BssAI RCCGGY 2 cut(s) 256, 320
BssECI CCNNGG 2 cut(s) 52, 73
BssMI GATC 2 cut(s) 69, 130
BssT1I CCWWGG 1 cut(s) 52
Bst2UI CCWGG 1 cut(s) 74
Bst4CI ACNGT 3 cut(s) 81, 125, 247
BstAPI GCANNNNNTGC 2 cut(s) 144, 239
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 1 cut(s) 32
BstF5I GGATG 1 cut(s) 62
BstKTI GATC 2 cut(s) 72, 133
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 2 cut(s) 69, 130
BstMWI GCNNNNNNNGC 5 cut(s) 57, 99, 144, 212, 239
BstNI CCWGG 1 cut(s) 74
BstNSI RCATGY 2 cut(s) 45, 341
BstSCI CCNGG 2 cut(s) 72, 109
BstSFI CTRYAG 1 cut(s) 358
BstSLI GKGCMC 1 cut(s) 252
BstV1I GCAGC 3 cut(s) 202, 245, 344
BstXI CCANNNNNNTGG 1 cut(s) 187
BsuRI GGCC 1 cut(s) 51
BtgZI GCGATG 1 cut(s) 60
BtrI CACGTC 1 cut(s) 199
BtsCI GGATG 1 cut(s) 62
Cac8I GCNNGC 1 cut(s) 43
Cfr10I RCCGGY 2 cut(s) 256, 320
Cfr13I GGNCC 1 cut(s) 116
CspAI ACCGGT 1 cut(s) 320
CviAII CATG 2 cut(s) 42, 338
CviJI RGCY 3 cut(s) 5, 51, 215
CviKI_1 RGCY 3 cut(s) 5, 51, 215
DdeI CTNAG 1 cut(s) 32
DpnI GATC 2 cut(s) 71, 132
DpnII GATC 2 cut(s) 69, 130
EciI GGCGGA 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 72
EcoT14I CCWWGG 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 52
Esp3I CGTCTC 1 cut(s) 205
FaeI CATG 2 cut(s) 45, 341
FaiI YATR 3 cut(s) 43, 339, 368
FaqI GGGAC 1 cut(s) 90
FatI CATG 2 cut(s) 41, 337
Fnu4HI GCNGC 5 cut(s) 216, 234, 261, 358, 361
FokI GGATG 1 cut(s) 69
Fsp4HI GCNGC 5 cut(s) 216, 234, 261, 358, 361
FspBI CTAG 2 cut(s) 6, 230
GluI GCNGC 5 cut(s) 216, 234, 261, 358, 361
HaeIII GGCC 1 cut(s) 51
HapII CCGG 4 cut(s) 111, 212, 257, 321
Hin1II CATG 2 cut(s) 45, 341
HpaII CCGG 4 cut(s) 111, 212, 257, 321
HphI GGTGA 1 cut(s) 161
Hpy188I TCNGA 1 cut(s) 33
HpyAV CCTTC 1 cut(s) 292
HpyCH4III ACNGT 3 cut(s) 81, 125, 247
HpyCH4IV ACGT 2 cut(s) 20, 198
HpyCH4V TGCA 5 cut(s) 60, 138, 218, 242, 360
HpyF10VI GCNNNNNNNGC 5 cut(s) 57, 99, 144, 212, 239
HpyF3I CTNAG 1 cut(s) 32
HpySE526I ACGT 2 cut(s) 20, 198
Hsp92II CATG 2 cut(s) 45, 341
Kzo9I GATC 2 cut(s) 69, 130
LmnI GCTCC 1 cut(s) 107
LpnPI CCDG 7 cut(s) 59, 86, 124, 157, 225, 270, 334
Lsp1109I GCAGC 3 cut(s) 202, 245, 344
LweI GCATC 2 cut(s) 47, 147
MaeI CTAG 2 cut(s) 6, 230
MaeII ACGT 2 cut(s) 20, 198
MaeIII GTNAC 1 cut(s) 340
MalI GATC 2 cut(s) 71, 132
MboI GATC 2 cut(s) 69, 130
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 2 cut(s) 220, 271
MnlI CCTC 4 cut(s) 27, 104, 182, 277
MslI CAYNNNNRTG 1 cut(s) 185
MspI CCGG 4 cut(s) 111, 212, 257, 321
MspR9I CCNGG 2 cut(s) 74, 111
MvaI CCWGG 1 cut(s) 74
MwoI GCNNNNNNNGC 5 cut(s) 57, 99, 144, 212, 239
NciI CCSGG 1 cut(s) 111
NdeII GATC 2 cut(s) 69, 130
NlaIII CATG 2 cut(s) 45, 341
NlaIV GGNNCC 1 cut(s) 249
NspI RCATGY 2 cut(s) 45, 341
OliI CACNNNNGTG 1 cut(s) 185
PaeI GCATGC 1 cut(s) 45
PciI ACATGT 1 cut(s) 337
PinAI ACCGGT 1 cut(s) 320
PkrI GCNGC 5 cut(s) 217, 235, 262, 359, 362
PscI ACATGT 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 72
PspGI CCWGG 1 cut(s) 72
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 1 cut(s) 116
PsrI GAACNNNNNNTAC 2 cut(s) 146, 178
PstI CTGCAG 1 cut(s) 362
RseI CAYNNNNRTG 1 cut(s) 185
SatI GCNGC 5 cut(s) 216, 234, 261, 358, 361
Sau3AI GATC 2 cut(s) 69, 130
Sau96I GGNCC 1 cut(s) 116
ScrFI CCNGG 2 cut(s) 74, 111
SduI GDGCHC 1 cut(s) 252
SetI ASST 4 cut(s) 23, 121, 174, 201
SfaNI GCATC 2 cut(s) 47, 147
SfcI CTRYAG 1 cut(s) 358
SinI GGWCC 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 185
SphI GCATGC 1 cut(s) 45
Sse9I AATT 2 cut(s) 220, 271
SsiI CCGC 2 cut(s) 114, 260
SspMI CTAG 2 cut(s) 6, 230
StyD4I CCNGG 2 cut(s) 72, 109
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 3 cut(s) 81, 125, 247
TaiI ACGT 2 cut(s) 23, 201
TaqI TCGA 2 cut(s) 96, 129
TasI AATT 2 cut(s) 220, 271
TauI GCSGC 1 cut(s) 263
TseI GCWGC 4 cut(s) 215, 233, 357, 360
VpaK11BI GGWCC 1 cut(s) 116
XceI RCATGY 2 cut(s) 45, 341
XspI CTAG 2 cut(s) 6, 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.