Rw4G004960

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0018414
Physical Location & Seq
Reverse (-)
9905108 .. 9905761
654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G004960.1

Sequence Viewer

Length: 384 bp
ATGGCTTGTTTTACTACTACCTTTTCATATCTGAGCGTGGCATGCGATGGCCTTGGATGCAATGCCTTGATTCAGGGACGTTATTTTACTTGCCTGGAATGCTCCATTCCCGGCGGACCTAACAGTTTCGATCTCTGCACCTCCTGCTATCCGAATCAAAACTACTCCCACCTCCACACCACTTTTGTGGAAAAGCACGTCTCTGCGTTTCCAGCTGCAAATTACCTACCAACATCGTATACAGCTGCGAGCCAACCGGCGACACTGAATTTCAGTAATGAGGCAGCAATGAAGCGAATAGAAGCAGTCAATACGGGTTGTAGTTATTGCACATTGCCACATTTTATAGATAATGTTAATGATGTCTGTTGCAGCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

127

Amino Acids

13.8

Weight (kDa)

5.3

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018414)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g04870
rosa_chinensis RchiOBHm_Chr4g0395651 RchiOBHm_Chr4g0395681
rosa_roxburghii Rroxscaffold_5G00340560
rosa_rugosa Rorug03G0355000
rosa_samantha Rh4BG061000 Rh4CG067000 Rh4CG067100 Rh4DG056400
rosa_wichuraiana Rw4G004960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 239
AciI CCGC 1 cut(s) 114
AcsI RAATTY 1 cut(s) 268
AjiI CACGTC 1 cut(s) 199
AjnI CCWGG 1 cut(s) 93
AleI CACNNNNGTG 1 cut(s) 185
AluBI AGCT 2 cut(s) 215, 245
AluI AGCT 2 cut(s) 215, 245
Alw26I GTCTC 1 cut(s) 205
AoxI GGCC 1 cut(s) 49
ApeKI GCWGC 4 cut(s) 215, 245, 284, 372
ApoI RAATTY 1 cut(s) 268
AspS9I GGNCC 1 cut(s) 116
AsuC2I CCSGG 1 cut(s) 111
AvaII GGWCC 1 cut(s) 116
BbvI GCAGC 3 cut(s) 202, 232, 296
BccI CCATC 1 cut(s) 41
BciT130I CCWGG 1 cut(s) 95
BcnI CCSGG 1 cut(s) 111
BcoDI GTCTC 1 cut(s) 205
BisI GCNGC 4 cut(s) 216, 246, 285, 373
BlsI GCNGC 4 cut(s) 217, 247, 286, 374
Bme1390I CCNGG 2 cut(s) 95, 111
Bme18I GGWCC 1 cut(s) 116
BmgBI CACGTC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 116
BmrFI CCNGG 2 cut(s) 95, 111
BmsI GCATC 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 111
BsaJI CCNNGG 1 cut(s) 52
Bse118I RCCGGY 1 cut(s) 256
Bse3DI GCAATG 3 cut(s) 67, 294, 332
BseBI CCWGG 1 cut(s) 95
BseDI CCNNGG 1 cut(s) 52
BseGI GGATG 1 cut(s) 62
BseMI GCAATG 3 cut(s) 67, 294, 332
BseMII CTCAG 1 cut(s) 23
BseXI GCAGC 3 cut(s) 202, 232, 296
BsgI GTGCAG 1 cut(s) 121
BshFI GGCC 1 cut(s) 51
BsiSI CCGG 2 cut(s) 111, 257
BslFI GGGAC 1 cut(s) 90
BsmAI GTCTC 1 cut(s) 205
BsmBI CGTCTC 1 cut(s) 205
BsmFI GGGAC 1 cut(s) 90
BsmI GAATGC 1 cut(s) 104
BsnI GGCC 1 cut(s) 51
Bsp143I GATC 1 cut(s) 130
BspACI CCGC 1 cut(s) 114
BspANI GGCC 1 cut(s) 51
BspCNI CTCAG 1 cut(s) 24
BsrDI GCAATG 3 cut(s) 67, 294, 332
BsrFI RCCGGY 1 cut(s) 256
BssAI RCCGGY 1 cut(s) 256
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 1 cut(s) 130
BssNAI GTATAC 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 52
Bst1107I GTATAC 1 cut(s) 240
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 1 cut(s) 125
BstAPI GCANNNNNTGC 1 cut(s) 144
BstC8I GCNNGC 2 cut(s) 43, 250
BstDEI CTNAG 1 cut(s) 32
BstF5I GGATG 1 cut(s) 62
BstKTI GATC 1 cut(s) 133
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 1 cut(s) 130
BstMWI GCNNNNNNNGC 5 cut(s) 42, 57, 99, 144, 212
BstNI CCWGG 1 cut(s) 95
BstNSI RCATGY 1 cut(s) 45
BstSCI CCNGG 2 cut(s) 93, 109
BstV1I GCAGC 3 cut(s) 202, 232, 296
BstXI CCANNNNNNTGG 1 cut(s) 187
BstZ17I GTATAC 1 cut(s) 240
BsuRI GGCC 1 cut(s) 51
BtgZI GCGATG 1 cut(s) 60
BtrI CACGTC 1 cut(s) 199
BtsCI GGATG 1 cut(s) 62
BtsIMutI CAGTG 1 cut(s) 263
Cac8I GCNNGC 2 cut(s) 43, 250
Cfr10I RCCGGY 1 cut(s) 256
Cfr13I GGNCC 1 cut(s) 116
CviAII CATG 1 cut(s) 42
CviJI RGCY 5 cut(s) 5, 51, 215, 245, 252
CviKI_1 RGCY 5 cut(s) 5, 51, 215, 245, 252
DdeI CTNAG 1 cut(s) 32
DpnI GATC 1 cut(s) 132
DpnII GATC 1 cut(s) 130
EciI GGCGGA 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 93
EcoT14I CCWWGG 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 52
Esp3I CGTCTC 1 cut(s) 205
FaeI CATG 1 cut(s) 45
FaiI YATR 5 cut(s) 28, 43, 240, 347, 380
FaqI GGGAC 1 cut(s) 90
FatI CATG 1 cut(s) 41
FblI GTMKAC 1 cut(s) 239
Fnu4HI GCNGC 4 cut(s) 216, 246, 285, 373
FokI GGATG 1 cut(s) 69
Fsp4HI GCNGC 4 cut(s) 216, 246, 285, 373
GluI GCNGC 4 cut(s) 216, 246, 285, 373
HaeIII GGCC 1 cut(s) 51
HapII CCGG 2 cut(s) 111, 257
Hin1II CATG 1 cut(s) 45
HinfI GANTC 2 cut(s) 70, 154
HpaII CCGG 2 cut(s) 111, 257
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 2 cut(s) 33, 153
Hpy8I GTNNAC 1 cut(s) 240
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4IV ACGT 2 cut(s) 79, 198
HpyCH4V TGCA 5 cut(s) 60, 138, 218, 330, 372
HpyF10VI GCNNNNNNNGC 5 cut(s) 42, 57, 99, 144, 212
HpyF3I CTNAG 1 cut(s) 32
HpySE526I ACGT 2 cut(s) 79, 198
Hsp92II CATG 1 cut(s) 45
Kzo9I GATC 1 cut(s) 130
LmnI GCTCC 1 cut(s) 107
LpnPI CCDG 7 cut(s) 59, 80, 107, 124, 157, 225, 270
Lsp1109I GCAGC 3 cut(s) 202, 232, 296
LweI GCATC 1 cut(s) 47
MaeII ACGT 2 cut(s) 79, 198
MalI GATC 1 cut(s) 132
MboI GATC 1 cut(s) 130
MluCI AATT 2 cut(s) 220, 268
MnlI CCTC 3 cut(s) 151, 182, 274
MseI TTAA 1 cut(s) 357
MslI CAYNNNNRTG 1 cut(s) 185
MspA1I CMGCKG 2 cut(s) 215, 245
MspI CCGG 2 cut(s) 111, 257
MspR9I CCNGG 2 cut(s) 95, 111
Mva1269I GAATGC 1 cut(s) 104
MvaI CCWGG 1 cut(s) 95
MwoI GCNNNNNNNGC 5 cut(s) 42, 57, 99, 144, 212
NciI CCSGG 1 cut(s) 111
NdeII GATC 1 cut(s) 130
NlaIII CATG 1 cut(s) 45
NspI RCATGY 1 cut(s) 45
OliI CACNNNNGTG 1 cut(s) 185
PaeI GCATGC 1 cut(s) 45
PctI GAATGC 1 cut(s) 104
PfeI GAWTC 2 cut(s) 70, 154
PkrI GCNGC 4 cut(s) 217, 247, 286, 374
Psp6I CCWGG 1 cut(s) 93
PspGI CCWGG 1 cut(s) 93
PspPI GGNCC 1 cut(s) 116
PvuII CAGCTG 2 cut(s) 215, 245
RseI CAYNNNNRTG 1 cut(s) 185
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 4 cut(s) 216, 246, 285, 373
Sau3AI GATC 1 cut(s) 130
Sau96I GGNCC 1 cut(s) 116
ScrFI CCNGG 2 cut(s) 95, 111
SetI ASST 9 cut(s) 23, 82, 121, 143, 174, 201, 217, 228, 247
SfaNI GCATC 1 cut(s) 47
SinI GGWCC 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 185
SphI GCATGC 1 cut(s) 45
Sse9I AATT 2 cut(s) 220, 268
SsiI CCGC 1 cut(s) 114
StyD4I CCNGG 2 cut(s) 93, 109
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 1 cut(s) 125
TaiI ACGT 2 cut(s) 82, 201
TaqI TCGA 1 cut(s) 129
TasI AATT 2 cut(s) 220, 268
TfiI GAWTC 2 cut(s) 70, 154
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TscAI CASTG 1 cut(s) 270
TseI GCWGC 4 cut(s) 215, 245, 284, 372
TspDTI ATGAA 2 cut(s) 15, 305
TspRI CASTG 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 116
XapI RAATTY 1 cut(s) 268
XceI RCATGY 1 cut(s) 45
XmiI GTMKAC 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.