FvH4_4g20480

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016824
Physical Location & Seq
Reverse (-)
23724513 .. 23725121
609 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20480.t1

Sequence Viewer

Length: 546 bp
ATGAAGATCGTTTCAATATCTATGCCGCTGCTCACCATATTTATCATTCAAATCGTGCTTCTTCCAATTAGCCATTGCCGGGCAGATCTCATTGACCAAACGTGCAAACAAACTCCCAACTACAATCTATGTGTTTCTTCTCTAAAACCGAACCCTCGTAGCTCCGCTGCTAATGTCAAGGGCCTGGCCACCATAATGGTCGAGGTGCTTACTTCCAAGGCAAACGATACTCTGAACGAAATATGGGCGCAGCTTCTTAGGCAGGAAGACCCTGCAGTTAGAAATTGCTATGATCATTATGGTTACATGGTGGCAGCCTTCATCCCAGAGATATATGGAGATTTAACTTCAAGGGGTTTTGGTGGTAGGTTTGTACGTTACAACCCTGCACAGGCGGAGCGACGCTTGCACGATGATATTATCCGACGAGTTGATCATTGTCGGCATGGTTTTGGTAAAGGCCGCCGATCTCCATTCGCAAAGGAAATCAAAGCTACCCGTGAAGCTGCGGTTGTGGCAGCAGCAATTGTTAGGATATTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

182

Amino Acids

20.31

Weight (kDa)

9.47

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 29 - 151 9.6e-15 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016824)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20480 FvH4_4g20510
rosa_chinensis RchiOBHm_Chr4g0425961
rosa_laevigata RLG00000007328
rosa_multiflora Rmu_co8133392.1_g000001 Rmu_sc0002091.1_g000006 Rmu_ssc0000281.1_g000023
rosa_roxburghii Rroxscaffold_5G00367970
rosa_rugosa Rorug04G0207700
rosa_samantha Rh4AG264600 Rh4BG270500 Rh4CG283100 Rh4DG267100
rosa_wichuraiana Rw4G022850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 26, 165, 395, 463, 509
AcoI YGGCCR 1 cut(s) 186
AfaI GTAC 1 cut(s) 375
AfiI CCNNNNNNNGG 2 cut(s) 79, 391
AgsI TTSAA 3 cut(s) 15, 50, 351
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 4 cut(s) 162, 253, 494, 506
AluI AGCT 4 cut(s) 162, 253, 494, 506
AoxI GGCC 3 cut(s) 181, 186, 460
ApeKI GCWGC 7 cut(s) 28, 167, 250, 314, 506, 518, 521
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 1 cut(s) 181
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 25
BalI TGGCCA 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 273
BbvI GCAGC 7 cut(s) 15, 154, 262, 326, 493, 530, 533
BciT130I CCWGG 1 cut(s) 185
BclI TGATCA 2 cut(s) 292, 433
BcnI CCSGG 1 cut(s) 80
BfmI CTRYAG 1 cut(s) 273
BglII AGATCT 1 cut(s) 85
BisI GCNGC 9 cut(s) 26, 29, 168, 251, 315, 463, 507, 519, 522
BlsI GCNGC 9 cut(s) 27, 30, 169, 252, 316, 464, 508, 520, 523
Bme1390I CCNGG 2 cut(s) 80, 185
BmgT120I GGNCC 1 cut(s) 181
BmrFI CCNGG 2 cut(s) 80, 185
BpiI GAAGAC 1 cut(s) 273
BpuMI CCSGG 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 216
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 391
Bse3DI GCAATG 1 cut(s) 73
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 1 cut(s) 321
BseLI CCNNNNNNNGG 2 cut(s) 79, 391
BseMI GCAATG 1 cut(s) 73
BseXI GCAGC 7 cut(s) 15, 154, 262, 326, 493, 530, 533
BsgI GTGCAG 1 cut(s) 372
BshFI GGCC 3 cut(s) 183, 188, 462
BsiSI CCGG 1 cut(s) 79
BslI CCNNNNNNNGG 2 cut(s) 79, 391
BsnI GGCC 3 cut(s) 183, 188, 462
Bsp143I GATC 5 cut(s) 6, 85, 292, 433, 467
BspACI CCGC 5 cut(s) 26, 165, 395, 463, 509
BspANI GGCC 3 cut(s) 183, 188, 462
BspMAI CTGCAG 1 cut(s) 277
BsrDI GCAATG 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 5 cut(s) 6, 85, 292, 433, 467
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 185
BstC8I GCNNGC 1 cut(s) 407
BstDEI CTNAG 1 cut(s) 257
BstF5I GGATG 1 cut(s) 321
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 5 cut(s) 9, 88, 295, 436, 470
BstMBI GATC 5 cut(s) 6, 85, 292, 433, 467
BstMWI GCNNNNNNNGC 3 cut(s) 259, 406, 515
BstNI CCWGG 1 cut(s) 185
BstSCI CCNGG 2 cut(s) 78, 183
BstSFI CTRYAG 1 cut(s) 273
BstV1I GCAGC 7 cut(s) 15, 154, 262, 326, 493, 530, 533
BstV2I GAAGAC 1 cut(s) 273
BstX2I RGATCY 1 cut(s) 85
BstXI CCANNNNNNTGG 1 cut(s) 196
BstYI RGATCY 1 cut(s) 85
BsuRI GGCC 3 cut(s) 183, 188, 462
BtsCI GGATG 1 cut(s) 321
Cac8I GCNNGC 1 cut(s) 407
CfoI GCGC 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 181
CseI GACGC 1 cut(s) 411
Csp6I GTAC 1 cut(s) 374
CviAII CATG 2 cut(s) 307, 446
CviJI RGCY 9 cut(s) 72, 162, 183, 188, 253, 317, 462, 494, 506
CviKI_1 RGCY 9 cut(s) 72, 162, 183, 188, 253, 317, 462, 494, 506
CviQI GTAC 1 cut(s) 374
DdeI CTNAG 1 cut(s) 257
DpnI GATC 5 cut(s) 8, 87, 294, 435, 469
DpnII GATC 5 cut(s) 6, 85, 292, 433, 467
EaeI YGGCCR 1 cut(s) 186
EciI GGCGGA 1 cut(s) 410
Eco130I CCWWGG 1 cut(s) 216
EcoO109I RGGNCCY 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 2 cut(s) 310, 449
FatI CATG 2 cut(s) 306, 445
FbaI TGATCA 2 cut(s) 292, 433
Fnu4HI GCNGC 9 cut(s) 26, 29, 168, 251, 315, 463, 507, 519, 522
FokI GGATG 1 cut(s) 308
Fsp4HI GCNGC 9 cut(s) 26, 29, 168, 251, 315, 463, 507, 519, 522
GlaI GCGC 1 cut(s) 249
GluI GCNGC 9 cut(s) 26, 29, 168, 251, 315, 463, 507, 519, 522
HaeIII GGCC 3 cut(s) 183, 188, 462
HapII CCGG 1 cut(s) 79
HgaI GACGC 1 cut(s) 411
HhaI GCGC 1 cut(s) 250
Hin1II CATG 2 cut(s) 310, 449
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HpaII CCGG 1 cut(s) 79
HphI GGTGA 1 cut(s) 25
Hpy188I TCNGA 2 cut(s) 234, 425
Hpy99I CGWCG 2 cut(s) 405, 429
HpyAV CCTTC 1 cut(s) 328
HpyCH4IV ACGT 2 cut(s) 101, 376
HpyCH4V TGCA 4 cut(s) 105, 275, 389, 409
HpyF10VI GCNNNNNNNGC 3 cut(s) 259, 406, 515
HpyF3I CTNAG 1 cut(s) 257
HpySE526I ACGT 2 cut(s) 101, 376
Hsp92II CATG 2 cut(s) 310, 449
HspAI GCGC 1 cut(s) 248
Ksp22I TGATCA 2 cut(s) 292, 433
Kzo9I GATC 5 cut(s) 6, 85, 292, 433, 467
LmnI GCTCC 2 cut(s) 167, 397
LpnPI CCDG 8 cut(s) 92, 170, 197, 248, 285, 339, 377, 399
Lsp1109I GCAGC 7 cut(s) 15, 154, 262, 326, 493, 530, 533
MaeII ACGT 2 cut(s) 101, 376
MaeIII GTNAC 2 cut(s) 302, 377
MalI GATC 5 cut(s) 8, 87, 294, 435, 469
MboI GATC 5 cut(s) 6, 85, 292, 433, 467
MboII GAAGA 4 cut(s) 16, 53, 129, 278
MfeI CAATTG 1 cut(s) 525
MflI RGATCY 1 cut(s) 85
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 3 cut(s) 66, 283, 525
MluNI TGGCCA 1 cut(s) 188
MmeI TCCRAC 1 cut(s) 448
MnlI CCTC 2 cut(s) 165, 196
Mox20I TGGCCA 1 cut(s) 188
MscI TGGCCA 1 cut(s) 188
MseI TTAA 1 cut(s) 344
MslI CAYNNNNRTG 1 cut(s) 194
Msp20I TGGCCA 1 cut(s) 188
MspA1I CMGCKG 2 cut(s) 28, 167
MspI CCGG 1 cut(s) 79
MspR9I CCNGG 2 cut(s) 80, 185
MunI CAATTG 1 cut(s) 525
MvaI CCWGG 1 cut(s) 185
MwoI GCNNNNNNNGC 3 cut(s) 259, 406, 515
NciI CCSGG 1 cut(s) 80
NdeII GATC 5 cut(s) 6, 85, 292, 433, 467
NlaIII CATG 2 cut(s) 310, 449
PkrI GCNGC 9 cut(s) 27, 30, 169, 252, 316, 464, 508, 520, 523
Psp6I CCWGG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 183
PspPI GGNCC 1 cut(s) 181
PstI CTGCAG 1 cut(s) 277
PsuI RGATCY 1 cut(s) 85
RsaI GTAC 1 cut(s) 375
RsaNI GTAC 1 cut(s) 374
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 1 cut(s) 344
SatI GCNGC 9 cut(s) 26, 29, 168, 251, 315, 463, 507, 519, 522
Sau3AI GATC 5 cut(s) 6, 85, 292, 433, 467
Sau96I GGNCC 1 cut(s) 181
ScrFI CCNGG 2 cut(s) 80, 185
SetI ASST 8 cut(s) 104, 164, 207, 255, 371, 379, 496, 508
SfcI CTRYAG 1 cut(s) 273
SmiMI CAYNNNNRTG 1 cut(s) 194
Sse9I AATT 3 cut(s) 66, 283, 525
SsiI CCGC 5 cut(s) 26, 165, 395, 463, 509
StyD4I CCNGG 2 cut(s) 78, 183
StyI CCWWGG 1 cut(s) 216
TaiI ACGT 2 cut(s) 104, 379
TaqI TCGA 1 cut(s) 201
TasI AATT 3 cut(s) 66, 283, 525
TauI GCSGC 2 cut(s) 28, 465
Tru1I TTAA 1 cut(s) 344
Tru9I TTAA 1 cut(s) 344
TseI GCWGC 7 cut(s) 28, 167, 250, 314, 506, 518, 521
TspDTI ATGAA 2 cut(s) 17, 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.