Rorug04G0207700

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0016824
Physical Location & Seq
Forward (+)
36090851 .. 36117167
26317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0207700.1

Sequence Viewer

Length: 771 bp
ATGGATTTGAGGTCTGAAATCGATCGCCTTTCTCGCAAGCGCAAGGAGGACAATCTCCGACGCGTCGTTGAGACCTCGAAAGGAAATCTCCGCATCTTCGCTCTCGCTTCCTCCTCCTCCTCCGACCACGGAGACAACGGCGGCTCCAGAAAACGCATGAGGCGAGACCGCGGCTGCAACGCCGTCGTTTCAACCACCAAGCCCGCCGCGGAGCCCACCAGGCCCGTCAGATCCGCCGTGAAACCCTATGACGACGGTGGGGATCTGAATATCGGGGAGTTCGAGAAACACACAAAGGGCATCGGCATGAAATTGCTGATGAGGATGGGATATAACGGACGTGGACTCGGCAAAAACGAGGAAGGTATTGTGAGCCCAATTGAAGCCGAATTGAGGCCCAAAAATGAAGGTCTTGGTTTTGATTACAAGGAGGGGACTAAGCTGCGGCGGCGTCCCACATCGTTTGTGAAAGCCAAGGTGGTGCACAAAAGTGTCGCTGGTGGTGACAAAGATCACAAGAAGACCAAAACAGAAACAGCCAAAAAACCAAGCAGCCTAAACACTAGTACAGTGGAAGGACACAAGCCCAACGAGCAGCCTAATGAGGCAATTACCAGTGCCGCCGGGATGGGGAAGAAGACGAGTACAAAAGGTGTCGATTTGCTGGAAAAGGTGATGGCTGATATGAGAAGACCTCTTGATGGAATCTTGACCGACTTTGAGAATTTGAGTTTGAAAGATATAACCTCCAGGTGCATCTTAGCTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

256

Amino Acids

28.17

Weight (kDa)

9.86

Isoelectric Point (pI)

48.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-patch PF01585 98 - 139 9e-16 G-patch domain
G-patch_2 PF12656 101 - 142 4.5e-07 G-patch domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016824)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20480 FvH4_4g20510
rosa_chinensis RchiOBHm_Chr4g0425961
rosa_laevigata RLG00000007328
rosa_multiflora Rmu_co8133392.1_g000001 Rmu_sc0002091.1_g000006 Rmu_ssc0000281.1_g000023
rosa_roxburghii Rroxscaffold_5G00367970
rosa_rugosa Rorug04G0207700
rosa_samantha Rh4AG264600 Rh4BG270500 Rh4CG283100 Rh4DG267100
rosa_wichuraiana Rw4G022850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 63, 171, 209
AclWI GGATC 2 cut(s) 225, 270
AcsI RAATTY 1 cut(s) 724
AcyI GRCGYC 1 cut(s) 451
AfaI GTAC 2 cut(s) 568, 646
AfiI CCNNNNNNNGG 3 cut(s) 393, 630, 701
AflIII ACRYGT 1 cut(s) 61
AgsI TTSAA 3 cut(s) 192, 383, 736
AhlI ACTAGT 1 cut(s) 563
AjiI CACGTC 1 cut(s) 341
AjnI CCWGG 2 cut(s) 218, 749
AleI CACNNNNGTG 1 cut(s) 489
AluBI AGCT 2 cut(s) 442, 764
AluI AGCT 2 cut(s) 442, 764
Alw21I GWGCWC 1 cut(s) 486
Alw26I GTCTC 3 cut(s) 65, 126, 159
Alw44I GTGCAC 1 cut(s) 482
AlwI GGATC 2 cut(s) 225, 270
AoxI GGCC 2 cut(s) 221, 395
ApaLI GTGCAC 1 cut(s) 482
ApeKI GCWGC 4 cut(s) 174, 442, 552, 595
ApoI RAATTY 1 cut(s) 724
AspLEI GCGC 1 cut(s) 42
AspS9I GGNCC 2 cut(s) 222, 396
AsuC2I CCSGG 1 cut(s) 625
AsuHPI GGTGA 2 cut(s) 515, 685
BaeGI GKGCMC 1 cut(s) 486
BanII GRGCYC 2 cut(s) 216, 377
BbsI GAAGAC 3 cut(s) 527, 644, 697
Bbv12I GWGCWC 1 cut(s) 486
BbvI GCAGC 4 cut(s) 161, 429, 564, 607
BccI CCATC 4 cut(s) 319, 622, 670, 695
BceAI ACGGC 3 cut(s) 154, 167, 221
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 2 cut(s) 220, 751
BcnI CCSGG 1 cut(s) 625
BcoDI GTCTC 3 cut(s) 65, 126, 159
BcuI ACTAGT 1 cut(s) 563
BfaI CTAG 1 cut(s) 564
BglI GCCNNNNNGGC 1 cut(s) 220
Bme1390I CCNGG 3 cut(s) 220, 625, 751
BmgBI CACGTC 1 cut(s) 341
BmgT120I GGNCC 2 cut(s) 222, 396
BmiI GGNNCC 2 cut(s) 145, 213
BmrFI CCNGG 3 cut(s) 220, 625, 751
BmsI GCATC 3 cut(s) 102, 309, 765
BpiI GAAGAC 3 cut(s) 527, 644, 697
BplI GAGNNNNNCTC 2 cut(s) 679, 711
BpmI CTGGAG 2 cut(s) 130, 733
BpuMI CCSGG 1 cut(s) 625
Bsa29I ATCGAT 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 451
BsaI GGTCTC 2 cut(s) 65, 159
BsaJI CCNNGG 4 cut(s) 127, 169, 207, 474
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 3 cut(s) 393, 630, 701
Bse1I ACTGG 1 cut(s) 615
BseBI CCWGG 2 cut(s) 220, 751
BseCI ATCGAT 1 cut(s) 21
BseDI CCNNGG 4 cut(s) 127, 169, 207, 474
BseGI GGATG 2 cut(s) 330, 633
BseLI CCNNNNNNNGG 3 cut(s) 393, 630, 701
BseNI ACTGG 1 cut(s) 615
BseRI GAGGAG 3 cut(s) 103, 106, 109
BseSI GKGCMC 1 cut(s) 486
BseXI GCAGC 4 cut(s) 161, 429, 564, 607
Bsh1236I CGCG 3 cut(s) 63, 171, 209
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 2 cut(s) 223, 397
BshVI ATCGAT 1 cut(s) 21
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 1 cut(s) 624
BslFI GGGAC 2 cut(s) 438, 448
BslI CCNNNNNNNGG 3 cut(s) 393, 630, 701
BsmAI GTCTC 3 cut(s) 65, 126, 159
BsmFI GGGAC 2 cut(s) 438, 448
BsnI GGCC 2 cut(s) 223, 397
Bso31I GGTCTC 2 cut(s) 65, 159
Bsp1286I GDGCHC 3 cut(s) 216, 377, 486
Bsp143I GATC 4 cut(s) 22, 230, 262, 511
BspANI GGCC 2 cut(s) 223, 397
BspDI ATCGAT 1 cut(s) 21
BspFNI CGCG 3 cut(s) 63, 171, 209
BspLI GGNNCC 2 cut(s) 145, 213
BspPI GGATC 2 cut(s) 225, 270
BspTNI GGTCTC 2 cut(s) 65, 159
BsrI ACTGG 1 cut(s) 615
BssECI CCNNGG 4 cut(s) 127, 169, 207, 474
BssMI GATC 4 cut(s) 22, 230, 262, 511
BssNI GRCGYC 1 cut(s) 451
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 2 cut(s) 220, 751
Bst4CI ACNGT 2 cut(s) 257, 571
BstACI GRCGYC 1 cut(s) 451
BstC8I GCNNGC 2 cut(s) 38, 204
BstDEI CTNAG 2 cut(s) 438, 760
BstDSI CCRYGG 3 cut(s) 127, 169, 207
BstF5I GGATG 2 cut(s) 330, 633
BstFNI CGCG 3 cut(s) 63, 171, 209
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 4 cut(s) 25, 233, 265, 514
BstMAI GTCTC 3 cut(s) 65, 126, 159
BstMBI GATC 4 cut(s) 22, 230, 262, 511
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 4 cut(s) 33, 220, 448, 592
BstNI CCWGG 2 cut(s) 220, 751
BstSCI CCNGG 3 cut(s) 218, 623, 749
BstSLI GKGCMC 1 cut(s) 486
BstUI CGCG 3 cut(s) 63, 171, 209
BstV1I GCAGC 4 cut(s) 161, 429, 564, 607
BstV2I GAAGAC 3 cut(s) 527, 644, 697
BstX2I RGATCY 2 cut(s) 230, 262
BstYI RGATCY 2 cut(s) 230, 262
Bsu15I ATCGAT 1 cut(s) 21
BsuRI GGCC 2 cut(s) 223, 397
BsuTUI ATCGAT 1 cut(s) 21
BtgI CCRYGG 3 cut(s) 127, 169, 207
BtrI CACGTC 1 cut(s) 341
BtsCI GGATG 2 cut(s) 330, 633
BtsIMutI CAGTG 2 cut(s) 576, 622
Cac8I GCNNGC 2 cut(s) 38, 204
CfoI GCGC 1 cut(s) 42
Cfr13I GGNCC 2 cut(s) 222, 396
Cfr42I CCGCGG 2 cut(s) 172, 210
ClaI ATCGAT 1 cut(s) 21
CseI GACGC 3 cut(s) 52, 69, 440
Csp6I GTAC 2 cut(s) 567, 645
CspCI CAANNNNNGTGG 2 cut(s) 445, 480
CviAII CATG 2 cut(s) 157, 307
CviQI GTAC 2 cut(s) 567, 645
DdeI CTNAG 2 cut(s) 438, 760
DpnI GATC 4 cut(s) 24, 232, 264, 513
DpnII GATC 4 cut(s) 22, 230, 262, 511
EciI GGCGGA 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 474
Eco24I GRGCYC 2 cut(s) 216, 377
Eco31I GGTCTC 2 cut(s) 65, 159
EcoRII CCWGG 2 cut(s) 218, 749
EcoT14I CCWWGG 1 cut(s) 474
EcoT38I GRGCYC 2 cut(s) 216, 377
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 2 cut(s) 160, 310
FaiI YATR 7 cut(s) 158, 249, 308, 333, 686, 743, 767
FaqI GGGAC 2 cut(s) 438, 448
FatI CATG 2 cut(s) 156, 306
FauI CCCGC 1 cut(s) 211
FokI GGATG 2 cut(s) 337, 640
FriOI GRGCYC 2 cut(s) 216, 377
FspBI CTAG 1 cut(s) 564
GlaI GCGC 1 cut(s) 41
GsuI CTGGAG 2 cut(s) 130, 733
HaeIII GGCC 2 cut(s) 223, 397
HapII CCGG 1 cut(s) 624
HgaI GACGC 3 cut(s) 52, 69, 440
HhaI GCGC 1 cut(s) 42
Hin1I GRCGYC 1 cut(s) 451
Hin1II CATG 2 cut(s) 160, 310
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HinfI GANTC 2 cut(s) 345, 705
HpaII CCGG 1 cut(s) 624
HphI GGTGA 2 cut(s) 515, 685
Hpy166II GTNNAC 2 cut(s) 344, 484
Hpy188I TCNGA 5 cut(s) 16, 59, 124, 230, 267
Hpy188III TCNNGA 4 cut(s) 147, 283, 698, 709
Hpy8I GTNNAC 2 cut(s) 344, 484
Hpy99I CGWCG 4 cut(s) 63, 68, 188, 257
HpyAV CCTTC 3 cut(s) 356, 401, 569
HpyCH4III ACNGT 2 cut(s) 257, 571
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 3 cut(s) 177, 484, 756
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 220, 448, 592
HpyF3I CTNAG 2 cut(s) 438, 760
HpySE526I ACGT 1 cut(s) 340
Hsp92I GRCGYC 1 cut(s) 451
Hsp92II CATG 2 cut(s) 160, 310
HspAI GCGC 1 cut(s) 40
KspI CCGCGG 2 cut(s) 172, 210
Kzo9I GATC 4 cut(s) 22, 230, 262, 511
LmnI GCTCC 2 cut(s) 149, 211
LpnPI CCDG 9 cut(s) 160, 205, 232, 483, 628, 637, 650, 736, 763
Lsp1109I GCAGC 4 cut(s) 161, 429, 564, 607
LweI GCATC 3 cut(s) 102, 309, 765
MaeI CTAG 1 cut(s) 564
MaeII ACGT 1 cut(s) 340
MaeIII GTNAC 1 cut(s) 503
MalI GATC 4 cut(s) 24, 232, 264, 513
MboI GATC 4 cut(s) 22, 230, 262, 511
MboII GAAGA 5 cut(s) 88, 532, 646, 649, 702
MfeI CAATTG 1 cut(s) 378
MflI RGATCY 2 cut(s) 230, 262
MhlI GDGCHC 3 cut(s) 216, 377, 486
MluCI AATT 5 cut(s) 311, 378, 389, 609, 724
MluI ACGCGT 1 cut(s) 61
MlyI GAGTC 1 cut(s) 339
MmeI TCCRAC 2 cut(s) 82, 147
MslI CAYNNNNRTG 2 cut(s) 305, 489
MspA1I CMGCKG 2 cut(s) 171, 209
MspI CCGG 1 cut(s) 624
MspR9I CCNGG 3 cut(s) 220, 625, 751
MunI CAATTG 1 cut(s) 378
MvaI CCWGG 2 cut(s) 220, 751
MvnI CGCG 3 cut(s) 63, 171, 209
MwoI GCNNNNNNNGC 4 cut(s) 33, 220, 448, 592
NciI CCSGG 1 cut(s) 625
NdeII GATC 4 cut(s) 22, 230, 262, 511
NlaIII CATG 2 cut(s) 160, 310
NlaIV GGNNCC 2 cut(s) 145, 213
NmeAIII GCCGAG 1 cut(s) 327
NmuCI GTSAC 1 cut(s) 503
OliI CACNNNNGTG 1 cut(s) 489
PcsI WCGNNNNNNNCGW 3 cut(s) 160, 279, 354
PfeI GAWTC 1 cut(s) 705
Ple19I CGATCG 1 cut(s) 25
PleI GAGTC 1 cut(s) 339
PpsI GAGTC 1 cut(s) 339
Psp6I CCWGG 2 cut(s) 218, 749
PspGI CCWGG 2 cut(s) 218, 749
PspN4I GGNNCC 2 cut(s) 145, 213
PspPI GGNCC 2 cut(s) 222, 396
PsuI RGATCY 2 cut(s) 230, 262
PvuI CGATCG 1 cut(s) 25
RsaI GTAC 2 cut(s) 568, 646
RsaNI GTAC 2 cut(s) 567, 645
RseI CAYNNNNRTG 2 cut(s) 305, 489
SacII CCGCGG 2 cut(s) 172, 210
Sau3AI GATC 4 cut(s) 22, 230, 262, 511
Sau96I GGNCC 2 cut(s) 222, 396
SchI GAGTC 1 cut(s) 339
ScrFI CCNGG 3 cut(s) 220, 625, 751
SduI GDGCHC 3 cut(s) 216, 377, 486
SfaNI GCATC 3 cut(s) 102, 309, 765
Sfr303I CCGCGG 2 cut(s) 172, 210
SgrBI CCGCGG 2 cut(s) 172, 210
SmiMI CAYNNNNRTG 2 cut(s) 305, 489
SpeI ACTAGT 1 cut(s) 563
Sse9I AATT 5 cut(s) 311, 378, 389, 609, 724
SspMI CTAG 1 cut(s) 564
StyD4I CCNGG 3 cut(s) 218, 623, 749
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 2 cut(s) 257, 571
TaiI ACGT 1 cut(s) 343
TaqI TCGA 4 cut(s) 21, 77, 282, 657
TaqII GACCGA 1 cut(s) 728
TasI AATT 5 cut(s) 311, 378, 389, 609, 724
TatI WGTACW 2 cut(s) 566, 644
TauI GCSGC 6 cut(s) 144, 174, 209, 448, 451, 623
TfiI GAWTC 1 cut(s) 705
TscAI CASTG 2 cut(s) 576, 622
TseFI GTSAC 1 cut(s) 503
TseI GCWGC 4 cut(s) 174, 442, 552, 595
Tsp45I GTSAC 1 cut(s) 503
TspDTI ATGAA 2 cut(s) 323, 420
TspGWI ACGGA 2 cut(s) 144, 351
TspRI CASTG 2 cut(s) 576, 622
VneI GTGCAC 1 cut(s) 482
XapI RAATTY 1 cut(s) 724
XspI CTAG 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.