FvH4_4g20510

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016824
Physical Location & Seq
Reverse (-)
23730119 .. 23731077
959 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20510.t1

Sequence Viewer

Length: 585 bp
ATGAACGAAAGCATTTTCCAACCCTTTTGTCTTGCAAAGATGAAGATCGTTTCAACATCTGGGCCCCTACTCACCATATTTATCATTCAAATCGTGTTTCTTCCAATAAGCCATTGCAGGGCCGATCTCATTGACCAAACATGCAAACAAACACCCAACTACAACCTATGTGTTTCTTCTCTCAAATCGAACCCTCGTAGCTCCGCTACTGATGTCAAGGGCCTAGCCATCATAATGGTCGAGGTGCTTAAGTCCAAGGCAAATGAAACTCTGAACAAAATATGGGCGCAGCTTCTTAGGCATGAAGACCCTGCAGTCAGAAATTGCTATGATCATTATGGTTACATGGTGGGAGCTTTCATACGAGATATCTATGGAGATTTAACTTCAAGGGGTGTAGGTGGTAACTTTGTACGTTACAACCCTGCACAGGCGGAGCGACGCTTGCACGATGATGTTATCCGAAGAGTTGATCATTGTCGGCATGGTTTTGGTAAAGGCCGCCAATCTCCATTTGGAAAGGAAAACAAAGCCACCCGTGAAGCTGCGGTTGTGGCTGCAGCAATTGTTAGGATATTGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

195

Amino Acids

21.73

Weight (kDa)

9.43

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 42 - 187 1.2e-15 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016824)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20480 FvH4_4g20510
rosa_chinensis RchiOBHm_Chr4g0425961
rosa_laevigata RLG00000007328
rosa_multiflora Rmu_co8133392.1_g000001 Rmu_sc0002091.1_g000006 Rmu_ssc0000281.1_g000023
rosa_roxburghii Rroxscaffold_5G00367970
rosa_rugosa Rorug04G0207700
rosa_samantha Rh4AG264600 Rh4BG270500 Rh4CG283100 Rh4DG267100
rosa_wichuraiana Rw4G022850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 314
AciI CCGC 4 cut(s) 204, 434, 502, 548
AfaI GTAC 1 cut(s) 414
AfiI CCNNNNNNNGG 2 cut(s) 118, 430
AflII CTTAAG 1 cut(s) 248
AgsI TTSAA 3 cut(s) 54, 89, 390
AluBI AGCT 4 cut(s) 201, 292, 356, 545
AluI AGCT 4 cut(s) 201, 292, 356, 545
AoxI GGCC 4 cut(s) 62, 120, 220, 499
ApaI GGGCCC 1 cut(s) 66
ApeKI GCWGC 4 cut(s) 289, 545, 557, 560
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 4 cut(s) 62, 63, 120, 220
AsuHPI GGTGA 1 cut(s) 64
BaeGI GKGCMC 1 cut(s) 66
BanII GRGCYC 1 cut(s) 66
BbsI GAAGAC 1 cut(s) 312
BbvI GCAGC 4 cut(s) 301, 532, 544, 572
BccI CCATC 1 cut(s) 236
BclI TGATCA 2 cut(s) 331, 472
BfaI CTAG 1 cut(s) 224
BfmI CTRYAG 2 cut(s) 312, 558
BfrI CTTAAG 1 cut(s) 248
BisI GCNGC 5 cut(s) 290, 502, 546, 558, 561
BlsI GCNGC 5 cut(s) 291, 503, 547, 559, 562
BmgT120I GGNCC 4 cut(s) 62, 63, 120, 220
BmiI GGNNCC 2 cut(s) 64, 65
BpiI GAAGAC 1 cut(s) 312
BsaBI GATNNNNATC 2 cut(s) 44, 578
BsaJI CCNNGG 1 cut(s) 255
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 430
Bse3DI GCAATG 1 cut(s) 112
Bse8I GATNNNNATC 2 cut(s) 44, 578
BseDI CCNNGG 1 cut(s) 255
BseJI GATNNNNATC 2 cut(s) 44, 578
BseLI CCNNNNNNNGG 2 cut(s) 118, 430
BseMI GCAATG 1 cut(s) 112
BseSI GKGCMC 1 cut(s) 66
BseXI GCAGC 4 cut(s) 301, 532, 544, 572
BsgI GTGCAG 1 cut(s) 411
BshFI GGCC 4 cut(s) 64, 122, 222, 501
BslI CCNNNNNNNGG 2 cut(s) 118, 430
BsnI GGCC 4 cut(s) 64, 122, 222, 501
Bsp120I GGGCCC 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 5 cut(s) 45, 124, 331, 472, 579
BspACI CCGC 4 cut(s) 204, 434, 502, 548
BspANI GGCC 4 cut(s) 64, 122, 222, 501
BspLI GGNNCC 2 cut(s) 64, 65
BspMAI CTGCAG 2 cut(s) 316, 562
BspTI CTTAAG 1 cut(s) 248
BsrDI GCAATG 1 cut(s) 112
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 5 cut(s) 45, 124, 331, 472, 579
BssT1I CCWWGG 1 cut(s) 255
Bst6I CTCTTC 1 cut(s) 460
BstAFI CTTAAG 1 cut(s) 248
BstC8I GCNNGC 1 cut(s) 446
BstDEI CTNAG 1 cut(s) 296
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 5 cut(s) 48, 127, 334, 475, 582
BstMBI GATC 5 cut(s) 45, 124, 331, 472, 579
BstMWI GCNNNNNNNGC 3 cut(s) 298, 445, 554
BstNSI RCATGY 1 cut(s) 144
BstSFI CTRYAG 2 cut(s) 312, 558
BstSLI GKGCMC 1 cut(s) 66
BstV1I GCAGC 4 cut(s) 301, 532, 544, 572
BstV2I GAAGAC 1 cut(s) 312
BstXI CCANNNNNNTGG 1 cut(s) 235
BsuRI GGCC 4 cut(s) 64, 122, 222, 501
Cac8I GCNNGC 1 cut(s) 446
CfoI GCGC 1 cut(s) 289
Cfr13I GGNCC 4 cut(s) 62, 63, 120, 220
CseI GACGC 1 cut(s) 450
Csp6I GTAC 1 cut(s) 413
CviAII CATG 4 cut(s) 141, 302, 346, 485
CviQI GTAC 1 cut(s) 413
DdeI CTNAG 1 cut(s) 296
DpnI GATC 5 cut(s) 47, 126, 333, 474, 581
DpnII GATC 5 cut(s) 45, 124, 331, 472, 579
DrdI GACNNNNNNGTC 1 cut(s) 314
DseDI GACNNNNNNGTC 1 cut(s) 314
Eam1104I CTCTTC 1 cut(s) 460
EarI CTCTTC 1 cut(s) 460
EciI GGCGGA 1 cut(s) 449
Eco130I CCWWGG 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 66
Eco32I GATATC 1 cut(s) 370
EcoO109I RGGNCCY 2 cut(s) 63, 220
EcoRV GATATC 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 255
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 4 cut(s) 144, 305, 349, 488
FatI CATG 4 cut(s) 140, 301, 345, 484
FbaI TGATCA 2 cut(s) 331, 472
Fnu4HI GCNGC 5 cut(s) 290, 502, 546, 558, 561
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 5 cut(s) 290, 502, 546, 558, 561
FspBI CTAG 1 cut(s) 224
GlaI GCGC 1 cut(s) 288
GluI GCNGC 5 cut(s) 290, 502, 546, 558, 561
HaeIII GGCC 4 cut(s) 64, 122, 222, 501
HgaI GACGC 1 cut(s) 450
HhaI GCGC 1 cut(s) 289
Hin1II CATG 4 cut(s) 144, 305, 349, 488
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HphI GGTGA 1 cut(s) 64
Hpy188I TCNGA 4 cut(s) 273, 320, 464, 584
Hpy99I CGWCG 1 cut(s) 444
HpyCH4IV ACGT 1 cut(s) 415
HpyCH4V TGCA 7 cut(s) 35, 117, 144, 314, 428, 448, 560
HpyF10VI GCNNNNNNNGC 3 cut(s) 298, 445, 554
HpyF3I CTNAG 1 cut(s) 296
HpySE526I ACGT 1 cut(s) 415
Hsp92II CATG 4 cut(s) 144, 305, 349, 488
HspAI GCGC 1 cut(s) 287
Ksp22I TGATCA 2 cut(s) 331, 472
Kzo9I GATC 5 cut(s) 45, 124, 331, 472, 579
LmnI GCTCC 3 cut(s) 206, 353, 436
LpnPI CCDG 5 cut(s) 45, 103, 324, 416, 438
Lsp1109I GCAGC 4 cut(s) 301, 532, 544, 572
MaeI CTAG 1 cut(s) 224
MaeII ACGT 1 cut(s) 415
MaeIII GTNAC 3 cut(s) 341, 404, 416
MalI GATC 5 cut(s) 47, 126, 333, 474, 581
MboI GATC 5 cut(s) 45, 124, 331, 472, 579
MboII GAAGA 5 cut(s) 55, 92, 168, 317, 477
MfeI CAATTG 1 cut(s) 564
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 2 cut(s) 322, 564
MmeI TCCRAC 1 cut(s) 43
MnlI CCTC 2 cut(s) 204, 235
MseI TTAA 2 cut(s) 249, 383
MslI CAYNNNNRTG 2 cut(s) 233, 453
MspCI CTTAAG 1 cut(s) 248
MunI CAATTG 1 cut(s) 564
MwoI GCNNNNNNNGC 3 cut(s) 298, 445, 554
NdeII GATC 5 cut(s) 45, 124, 331, 472, 579
NlaIII CATG 4 cut(s) 144, 305, 349, 488
NlaIV GGNNCC 2 cut(s) 64, 65
NspI RCATGY 1 cut(s) 144
PkrI GCNGC 5 cut(s) 291, 503, 547, 559, 562
PspN4I GGNNCC 2 cut(s) 64, 65
PspOMI GGGCCC 1 cut(s) 62
PspPI GGNCC 4 cut(s) 62, 63, 120, 220
PstI CTGCAG 2 cut(s) 316, 562
RsaI GTAC 1 cut(s) 414
RsaNI GTAC 1 cut(s) 413
RseI CAYNNNNRTG 2 cut(s) 233, 453
SaqAI TTAA 2 cut(s) 249, 383
SatI GCNGC 5 cut(s) 290, 502, 546, 558, 561
Sau3AI GATC 5 cut(s) 45, 124, 331, 472, 579
Sau96I GGNCC 4 cut(s) 62, 63, 120, 220
SduI GDGCHC 1 cut(s) 66
SetI ASST 8 cut(s) 168, 203, 246, 294, 358, 403, 418, 547
SfcI CTRYAG 2 cut(s) 312, 558
SmiMI CAYNNNNRTG 2 cut(s) 233, 453
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 2 cut(s) 322, 564
SsiI CCGC 4 cut(s) 204, 434, 502, 548
SspMI CTAG 1 cut(s) 224
StyI CCWWGG 1 cut(s) 255
TaiI ACGT 1 cut(s) 418
TaqI TCGA 2 cut(s) 188, 240
TasI AATT 2 cut(s) 322, 564
TauI GCSGC 1 cut(s) 504
Tru1I TTAA 2 cut(s) 249, 383
Tru9I TTAA 2 cut(s) 249, 383
TseI GCWGC 4 cut(s) 289, 545, 557, 560
TspDTI ATGAA 5 cut(s) 17, 56, 279, 318, 349
Vha464I CTTAAG 1 cut(s) 248
XceI RCATGY 1 cut(s) 144
XcmI CCANNNNNNNNNTGG 1 cut(s) 512
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.