FvH4_4g23640

4-coumarate--CoA ligase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017194
Physical Location & Seq
Forward (+)
26074962 .. 26077446
2485 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23640.t1

Sequence Viewer

Length: 1653 bp
ATGGGAACATTTGTGCAGAGTTCAGCCCAAGATGTGCAAGAACATATATTCCGAAGCCAAATGCCCGCTGTCCAGGTGCCTGATAACCTCACATTGCCAGAATTTGTGCTCAAAGATGCCGAGTTGTATGCTGACAATGTTGCCTTTGTGGAGGCTGTGACTGGAAAGAAAATCACTTACCGTGAGGTGGTTAGAGACACTGGTAGGTTTGCCAAGGCCTTAAGGTCTCTAGGCCTTAGAAAGGGGCATGTGGTCATTGTTGTTCTCCCCAATGTAGCTGAGTATGCCATTGTTGCCTTGGGAATAATGGCTGCTGGTGGTGTTTTTTCGGGTGCAAACCCCACGGGGCATGCATCCGAAATAAAAAAACAGGTAGTGGCTGCTGATGCCAAGCTAATTGTAACAAATGGAGCTAGCTATGAAAAGGTAAAAGCTTTCGGGGTACCAGTAATTGCAATCGGTGAAGAGACCATTGAAGGTGCTACGAATTGGTACAAATTGCTTGAAGCAGCAGACCGCGCCGGAACTAATCACTATCAGGAGAAAGTCCAACAGACTGATCTATGTGCTCTTCCCTTCTCATCAGGCACAACAGGACTATCCAAAGGTGTAATGCTGACTCACCGAAATCTTGTCGCTAACCTCAGCTCCACACTCTTTGGAGTAACGCGGGAAATGTTGGGAAAAGTCACTGTACTGGGCCTAATTCCCTTCTTCCACATCTATGGGATCACCGGAATATGTTGCGCTACGCTTAGAAACAAGGGGAAAGTTGTGGTGATGGGAAGATACGAACTTAGAACCTTTCTGAACGCATTGATCTCAGAAGAGATCACGTTTGCCCCCATTGTGCCACCAATCATTTTGAACTTAGTGAAGCATCCAGTAGTGGATGAATTCGATCTTAGCAAACTGAAACTCCAGGCTGTTATGACTGCAGCTGCTCCACTCGCACCGGATCTCCTCAAAGCATTCGAAAGCAAGTTCCCTGGAGTCCAAGTCCAAGAGGCATATGGACTGACTGAGCATAGCTGCATAACTCTGACTCATGCCGGACAGGGTTTTAAGGCAAAGAGAAACTCAGTTGGGTTTATTCTTCCAAACATGGAAGTGAAATTCATTGATCCTGAAACTGGTAGATCACTACCAAGAAACACCCCCGGTGAAATTTGTGTGTTGAGTCAATGCACCATGCAAGGTTACTACAACAATGAAGAAGAGACTATGAGGACCATTGACAAGAATGGTTGGCTTCACACTGGTGATGTTGGCTACATTGATGATGATGATAATGTCTTCATTGTCGATCGCATCAAGGAATTGATCAAATTCAAAGGTTTCCAAGTAGCTCCGGCTGAACTTGAGGCCATCCTTCTGAGTCATCCCTCAGTCGAAGACGCAGCAGTAGTCCCCCTGCCAGATGAGGAAGCAGGGGAGATACCAGCAGCATGTGTTGTGAAGGCAGCTGGTGCAAAAGAGAGTGAAGAAGACATAATCAACTATGTGGCCTCAAATGTTGCACATTACAAGAAAGTAAGAGTGGTGCACTTTGTGGACACCATTCCAAAATCACCCTCTGGAAAAATCATGAGGAGACTTGTGAAGGACACAATGATAGAAAAGATCAAGGCATCCAAGCTCAGCAGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001676 GO:0003674 GO:0003824 GO:0004467 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006082 GO:0006139 GO:0006163 GO:0006164 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009259 GO:0009260 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010584 GO:0010927 GO:0012505 GO:0015645 GO:0016043 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019752 GO:0022607 GO:0030198 GO:0031956 GO:0032501 GO:0032502 GO:0032787 GO:0032989 GO:0033865 GO:0033866 GO:0033875 GO:0034030 GO:0034032 GO:0034033 GO:0034641 GO:0034654 GO:0035337 GO:0035383 GO:0035384 GO:0043062 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046390 GO:0046483 GO:0046949 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0051186 GO:0051188 GO:0055086 GO:0071616 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0080110 GO:0085029 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901568 GO:1901570 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00130 ko00360 ko00940 ko01100 ko01110 map00130 map00360 map00940 map01100 map01110
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

551

Amino Acids

59.85

Weight (kDa)

6.38

Isoelectric Point (pI)

33.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 39 - 402 3.8e-79 AMP-binding enzyme
AMP-binding_C PF13193 453 - 528 5.6e-19 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62940
fragaria_vesca FvH4_4g23640
malus_domestica MD13G1120400.v1.1
prunus_persica Prupe.1G224800_v2.0.a1
pyrus_communis pycom13g10480
rosa_chinensis RchiOBHm_Chr4g0430101
rosa_laevigata RLG00000007003
rosa_multiflora Rmu_ssc0000150.1_g000024
rosa_roxburghii Rroxscaffold_5G00371920
rosa_rugosa Rorug04G0240200
rosa_samantha Rh4AG297300 Rh4BG303700
rosa_wichuraiana Rw4G025780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 442
AccB1I GGYRCC 2 cut(s) 76, 442
AccII CGCG 2 cut(s) 519, 670
AciI CCGC 3 cut(s) 66, 517, 670
AclWI GGATC 3 cut(s) 737, 966, 1118
AcsI RAATTY 5 cut(s) 101, 896, 1115, 1167, 1328
AdeI CACNNNGTG 1 cut(s) 1552
AfaI GTAC 3 cut(s) 444, 494, 696
AfiI CCNNNNNNNGG 3 cut(s) 187, 241, 1133
AflII CTTAAG 1 cut(s) 220
AgsI TTSAA 4 cut(s) 476, 506, 868, 1333
AjnI CCWGG 3 cut(s) 72, 921, 988
AleI CACNNNNGTG 1 cut(s) 1260
AloI GAACNNNNNNTCC 2 cut(s) 33, 65
Alw21I GWGCWC 3 cut(s) 111, 571, 1548
Alw26I GTCTC 5 cut(s) 189, 231, 461, 1214, 1588
Alw44I GTGCAC 1 cut(s) 1544
AlwI GGATC 3 cut(s) 737, 966, 1118
AoxI GGCC 5 cut(s) 216, 232, 700, 1365, 1506
ApaLI GTGCAC 1 cut(s) 1544
ApoI RAATTY 5 cut(s) 101, 896, 1115, 1167, 1328
Asp718I GGTACC 1 cut(s) 442
AspLEI GCGC 2 cut(s) 521, 749
AspS9I GGNCC 2 cut(s) 700, 1230
AsuC2I CCSGG 1 cut(s) 1161
AsuHPI GGTGA 7 cut(s) 473, 614, 724, 790, 1175, 1274, 1563
AsuII TTCGAA 1 cut(s) 975
AsuNHI GCTAGC 1 cut(s) 413
AvaII GGWCC 1 cut(s) 1230
BaeGI GKGCMC 1 cut(s) 1548
BanI GGYRCC 2 cut(s) 76, 442
BbsI GAAGAC 3 cut(s) 1288, 1401, 1494
Bbv12I GWGCWC 3 cut(s) 111, 571, 1548
BbvCI CCTCAGC 1 cut(s) 644
BbvI GCAGC 9 cut(s) 298, 367, 521, 928, 950, 1019, 1412, 1457, 1475
BccI CCATC 2 cut(s) 775, 1376
BcgI CGANNNNNNTGC 2 cut(s) 110, 144
BciT130I CCWGG 3 cut(s) 74, 923, 990
BclI TGATCA 1 cut(s) 1323
BcnI CCSGG 1 cut(s) 1161
BcoDI GTCTC 5 cut(s) 189, 231, 461, 1214, 1588
BfaI CTAG 2 cut(s) 230, 414
BfmI CTRYAG 1 cut(s) 936
BfrI CTTAAG 1 cut(s) 220
BlpI GCTNAGC 1 cut(s) 1640
Bme1390I CCNGG 4 cut(s) 74, 923, 990, 1161
Bme18I GGWCC 1 cut(s) 1230
BmgT120I GGNCC 2 cut(s) 700, 1230
BmiI GGNNCC 2 cut(s) 78, 444
BmrFI CCNGG 4 cut(s) 74, 923, 990, 1161
BmrI ACTGGG 1 cut(s) 707
BmsI GCATC 6 cut(s) 106, 362, 376, 889, 1320, 1640
BmtI GCTAGC 1 cut(s) 417
BmuI ACTGGG 1 cut(s) 707
BpiI GAAGAC 3 cut(s) 1288, 1401, 1494
BpmI CTGGAG 2 cut(s) 905, 1011
Bpu10I CCTNAGC 1 cut(s) 644
Bpu1102I GCTNAGC 1 cut(s) 1640
Bpu14I TTCGAA 1 cut(s) 975
BpuEI CTTGAG 1 cut(s) 1382
BpuMI CCSGG 1 cut(s) 1161
BsaI GGTCTC 2 cut(s) 231, 461
BsaJI CCNNGG 5 cut(s) 213, 297, 342, 988, 1159
BsaWI WCCGGW 2 cut(s) 734, 955
BsaXI ACNNNNNCTCC 8 cut(s) 632, 662, 903, 933, 945, 975, 984, 1014
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 241, 1133
Bse1I ACTGG 7 cut(s) 166, 205, 446, 702, 884, 1138, 1264
Bse3DI GCAATG 1 cut(s) 92
BseBI CCWGG 3 cut(s) 74, 923, 990
BseDI CCNNGG 5 cut(s) 213, 297, 342, 988, 1159
BseGI GGATG 6 cut(s) 353, 880, 898, 1368, 1381, 1631
BseLI CCNNNNNNNGG 3 cut(s) 187, 241, 1133
BseMI GCAATG 1 cut(s) 92
BseMII CTCAG 7 cut(s) 270, 658, 837, 1014, 1095, 1367, 1401
BseNI ACTGG 7 cut(s) 166, 205, 446, 702, 884, 1138, 1264
BseRI GAGGAG 2 cut(s) 953, 1606
BseSI GKGCMC 1 cut(s) 1548
BseXI GCAGC 9 cut(s) 298, 367, 521, 928, 950, 1019, 1412, 1457, 1475
BsgI GTGCAG 1 cut(s) 35
Bsh1236I CGCG 2 cut(s) 519, 670
Bsh1285I CGRYCG 1 cut(s) 1309
BshFI GGCC 5 cut(s) 218, 234, 702, 1367, 1508
BshNI GGYRCC 2 cut(s) 76, 442
BsiEI CGRYCG 1 cut(s) 1309
BsiHKAI GWGCWC 3 cut(s) 111, 571, 1548
BsiSI CCGG 6 cut(s) 522, 735, 956, 1053, 1161, 1352
BslFI GGGAC 1 cut(s) 1394
BslI CCNNNNNNNGG 3 cut(s) 187, 241, 1133
BsmAI GTCTC 5 cut(s) 189, 231, 461, 1214, 1588
BsmFI GGGAC 1 cut(s) 1394
BsmI GAATGC 1 cut(s) 971
BsnI GGCC 5 cut(s) 218, 234, 702, 1367, 1508
Bso31I GGTCTC 2 cut(s) 231, 461
Bsp119I TTCGAA 1 cut(s) 975
Bsp1286I GDGCHC 3 cut(s) 111, 571, 1548
Bsp1720I GCTNAGC 1 cut(s) 1640
BspACI CCGC 3 cut(s) 66, 517, 670
BspANI GGCC 5 cut(s) 218, 234, 702, 1367, 1508
BspCNI CTCAG 8 cut(s) 271, 657, 836, 1015, 1094, 1368, 1400, 1653
BspFNI CGCG 2 cut(s) 519, 670
BspHI TCATGA 1 cut(s) 1587
BspLI GGNNCC 2 cut(s) 78, 444
BspMAI CTGCAG 1 cut(s) 940
BspOI GCTAGC 1 cut(s) 417
BspPI GGATC 3 cut(s) 737, 966, 1118
BspQI GCTCTTC 1 cut(s) 576
BspT104I TTCGAA 1 cut(s) 975
BspT107I GGYRCC 2 cut(s) 76, 442
BspTI CTTAAG 1 cut(s) 220
BspTNI GGTCTC 2 cut(s) 231, 461
BsrDI GCAATG 1 cut(s) 92
BsrI ACTGG 7 cut(s) 166, 205, 446, 702, 884, 1138, 1264
BssECI CCNNGG 5 cut(s) 213, 297, 342, 988, 1159
BssT1I CCWWGG 2 cut(s) 213, 297
Bst2UI CCWGG 3 cut(s) 74, 923, 990
Bst4CI ACNGT 2 cut(s) 182, 694
Bst6I CTCTTC 4 cut(s) 459, 576, 822, 1212
BstAFI CTTAAG 1 cut(s) 220
BstAPI GCANNNNNTGC 1 cut(s) 1469
BstBI TTCGAA 1 cut(s) 975
BstC8I GCNNGC 3 cut(s) 66, 351, 415
BstDSI CCRYGG 1 cut(s) 342
BstENI CCTNNNNNAGG 1 cut(s) 239
BstF5I GGATG 6 cut(s) 353, 880, 898, 1368, 1381, 1631
BstFNI CGCG 2 cut(s) 519, 670
BstHHI GCGC 2 cut(s) 521, 749
BstMAI GTCTC 5 cut(s) 189, 231, 461, 1214, 1588
BstMCI CGRYCG 1 cut(s) 1309
BstMWI GCNNNNNNNGC 6 cut(s) 284, 293, 386, 518, 950, 1469
BstNI CCWGG 3 cut(s) 74, 923, 990
BstNSI RCATGY 3 cut(s) 251, 353, 1452
BstSCI CCNGG 4 cut(s) 72, 921, 988, 1159
BstSFI CTRYAG 1 cut(s) 936
BstSLI GKGCMC 1 cut(s) 1548
BstUI CGCG 2 cut(s) 519, 670
BstV1I GCAGC 9 cut(s) 298, 367, 521, 928, 950, 1019, 1412, 1457, 1475
BstV2I GAAGAC 3 cut(s) 1288, 1401, 1494
BstX2I RGATCY 1 cut(s) 958
BstXI CCANNNNNNTGG 1 cut(s) 725
BstYI RGATCY 1 cut(s) 958
BsuRI GGCC 5 cut(s) 218, 234, 702, 1367, 1508
BtgI CCRYGG 1 cut(s) 342
BtsCI GGATG 6 cut(s) 353, 880, 898, 1368, 1381, 1631
BtsIMutI CAGTG 3 cut(s) 198, 690, 1257
Cac8I GCNNGC 3 cut(s) 66, 351, 415
CciI TCATGA 1 cut(s) 1587
CfoI GCGC 2 cut(s) 521, 749
Cfr13I GGNCC 2 cut(s) 700, 1230
CseI GACGC 1 cut(s) 1406
Csp6I GTAC 3 cut(s) 443, 493, 695
CspCI CAANNNNNGTGG 2 cut(s) 640, 675
CviAII CATG 7 cut(s) 248, 350, 1049, 1105, 1192, 1449, 1588
CviQI GTAC 3 cut(s) 443, 493, 695
DraIII CACNNNGTG 1 cut(s) 1552
Eam1104I CTCTTC 4 cut(s) 459, 576, 822, 1212
EarI CTCTTC 4 cut(s) 459, 576, 822, 1212
Eco130I CCWWGG 2 cut(s) 213, 297
Eco147I AGGCCT 2 cut(s) 218, 234
Eco31I GGTCTC 2 cut(s) 231, 461
Eco47I GGWCC 1 cut(s) 1230
EcoNI CCTNNNNNAGG 1 cut(s) 239
EcoRI GAATTC 1 cut(s) 896
EcoRII CCWGG 3 cut(s) 72, 921, 988
EcoT14I CCWWGG 2 cut(s) 213, 297
EcoT22I ATGCAT 1 cut(s) 355
ErhI CCWWGG 2 cut(s) 213, 297
FaeI CATG 7 cut(s) 251, 353, 1052, 1108, 1195, 1452, 1591
FaqI GGGAC 1 cut(s) 1394
FatI CATG 7 cut(s) 247, 349, 1048, 1104, 1191, 1448, 1587
FauI CCCGC 2 cut(s) 73, 663
FauNDI CATATG 1 cut(s) 1012
FbaI TGATCA 1 cut(s) 1323
FokI GGATG 6 cut(s) 340, 867, 905, 1355, 1368, 1618
FspBI CTAG 2 cut(s) 230, 414
GlaI GCGC 2 cut(s) 520, 748
GsuI CTGGAG 2 cut(s) 905, 1011
HaeIII GGCC 5 cut(s) 218, 234, 702, 1367, 1508
HapII CCGG 6 cut(s) 522, 735, 956, 1053, 1161, 1352
HgaI GACGC 1 cut(s) 1406
HhaI GCGC 2 cut(s) 521, 749
Hin1II CATG 7 cut(s) 251, 353, 1052, 1108, 1195, 1452, 1591
Hin6I GCGC 2 cut(s) 519, 747
HinP1I GCGC 2 cut(s) 519, 747
HindIII AAGCTT 1 cut(s) 432
HinfI GANTC 5 cut(s) 619, 993, 1045, 1180, 1378
HpaII CCGG 6 cut(s) 522, 735, 956, 1053, 1161, 1352
HphI GGTGA 7 cut(s) 473, 614, 724, 790, 1175, 1274, 1563
Hpy166II GTNNAC 2 cut(s) 1546, 1555
Hpy188I TCNGA 6 cut(s) 53, 358, 810, 826, 1044, 1377
Hpy188III TCNNGA 4 cut(s) 539, 1127, 1578, 1588
Hpy8I GTNNAC 2 cut(s) 1546, 1555
HpyAV CCTTC 6 cut(s) 470, 586, 721, 1382, 1453, 1597
HpyCH4III ACNGT 2 cut(s) 182, 694
HpyCH4IV ACGT 1 cut(s) 836
HpyF10VI GCNNNNNNNGC 6 cut(s) 284, 293, 386, 518, 950, 1469
HpySE526I ACGT 1 cut(s) 836
Hsp92II CATG 7 cut(s) 251, 353, 1052, 1108, 1195, 1452, 1591
HspAI GCGC 2 cut(s) 519, 747
KpnI GGTACC 1 cut(s) 446
Ksp22I TGATCA 1 cut(s) 1323
LguI GCTCTTC 1 cut(s) 576
LmnI GCTCC 4 cut(s) 410, 653, 949, 1354
Lsp1109I GCAGC 9 cut(s) 298, 367, 521, 928, 950, 1019, 1412, 1457, 1475
LweI GCATC 6 cut(s) 106, 362, 376, 889, 1320, 1640
MaeI CTAG 2 cut(s) 230, 414
MaeII ACGT 1 cut(s) 836
MaeIII GTNAC 5 cut(s) 157, 400, 664, 688, 1199
MflI RGATCY 1 cut(s) 958
MhlI GDGCHC 3 cut(s) 111, 571, 1548
MlyI GAGTC 5 cut(s) 613, 1002, 1039, 1189, 1387
MmeI TCCRAC 1 cut(s) 574
Mph1103I ATGCAT 1 cut(s) 355
MseI TTAA 2 cut(s) 221, 1065
MslI CAYNNNNRTG 3 cut(s) 723, 1109, 1260
MspA1I CMGCKG 3 cut(s) 68, 941, 1466
MspCI CTTAAG 1 cut(s) 220
MspI CCGG 6 cut(s) 522, 735, 956, 1053, 1161, 1352
MspR9I CCNGG 4 cut(s) 74, 923, 990, 1161
Mva1269I GAATGC 1 cut(s) 971
MvaI CCWGG 3 cut(s) 74, 923, 990
MvnI CGCG 2 cut(s) 519, 670
MwoI GCNNNNNNNGC 6 cut(s) 284, 293, 386, 518, 950, 1469
NciI CCSGG 1 cut(s) 1161
NdeI CATATG 1 cut(s) 1012
NheI GCTAGC 1 cut(s) 413
NlaIII CATG 7 cut(s) 251, 353, 1052, 1108, 1195, 1452, 1591
NlaIV GGNNCC 2 cut(s) 78, 444
NmeAIII GCCGAG 1 cut(s) 145
NmuCI GTSAC 2 cut(s) 157, 688
NsiI ATGCAT 1 cut(s) 355
NspI RCATGY 3 cut(s) 251, 353, 1452
NspV TTCGAA 1 cut(s) 975
OliI CACNNNNGTG 1 cut(s) 1260
PaeI GCATGC 1 cut(s) 353
PagI TCATGA 1 cut(s) 1587
PceI AGGCCT 2 cut(s) 218, 234
PciSI GCTCTTC 1 cut(s) 576
PctI GAATGC 1 cut(s) 971
Ple19I CGATCG 1 cut(s) 1309
PleI GAGTC 5 cut(s) 613, 1001, 1039, 1188, 1386
PpsI GAGTC 5 cut(s) 613, 1001, 1039, 1188, 1386
Psp6I CCWGG 3 cut(s) 72, 921, 988
PspGI CCWGG 3 cut(s) 72, 921, 988
PspN4I GGNNCC 2 cut(s) 78, 444
PspPI GGNCC 2 cut(s) 700, 1230
PstI CTGCAG 1 cut(s) 940
PsuI RGATCY 1 cut(s) 958
PvuI CGATCG 1 cut(s) 1309
PvuII CAGCTG 2 cut(s) 941, 1466
RsaI GTAC 3 cut(s) 444, 494, 696
RsaNI GTAC 3 cut(s) 443, 493, 695
RseI CAYNNNNRTG 3 cut(s) 723, 1109, 1260
SapI GCTCTTC 1 cut(s) 576
SaqAI TTAA 2 cut(s) 221, 1065
Sau96I GGNCC 2 cut(s) 700, 1230
SchI GAGTC 5 cut(s) 613, 1002, 1039, 1189, 1387
ScrFI CCNGG 4 cut(s) 74, 923, 990, 1161
SduI GDGCHC 3 cut(s) 111, 571, 1548
SfaNI GCATC 6 cut(s) 106, 362, 376, 889, 1320, 1640
SfcI CTRYAG 1 cut(s) 936
SfuI TTCGAA 1 cut(s) 975
SinI GGWCC 1 cut(s) 1230
SmiMI CAYNNNNRTG 3 cut(s) 723, 1109, 1260
SmlI CTYRAG 2 cut(s) 220, 1361
SmoI CTYRAG 2 cut(s) 220, 1361
SphI GCATGC 1 cut(s) 353
SseBI AGGCCT 2 cut(s) 218, 234
SsiI CCGC 3 cut(s) 66, 517, 670
SspMI CTAG 2 cut(s) 230, 414
StuI AGGCCT 2 cut(s) 218, 234
StyD4I CCNGG 4 cut(s) 72, 921, 988, 1159
StyI CCWWGG 2 cut(s) 213, 297
TaaI ACNGT 2 cut(s) 182, 694
TaiI ACGT 1 cut(s) 839
TaqI TCGA 4 cut(s) 900, 975, 1305, 1392
TatI WGTACW 1 cut(s) 694
Tru1I TTAA 2 cut(s) 221, 1065
Tru9I TTAA 2 cut(s) 221, 1065
TscAI CASTG 3 cut(s) 205, 697, 1264
TseFI GTSAC 2 cut(s) 157, 688
Tsp45I GTSAC 2 cut(s) 157, 688
TspDTI ATGAA 5 cut(s) 435, 909, 1108, 1227, 1288
TspRI CASTG 3 cut(s) 205, 697, 1264
Vha464I CTTAAG 1 cut(s) 220
VneI GTGCAC 1 cut(s) 1544
VpaK11BI GGWCC 1 cut(s) 1230
XagI CCTNNNNNAGG 1 cut(s) 239
XapI RAATTY 5 cut(s) 101, 896, 1115, 1167, 1328
XceI RCATGY 3 cut(s) 251, 353, 1452
XcmI CCANNNNNNNNNTGG 2 cut(s) 295, 1010
XspI CTAG 2 cut(s) 230, 414
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.