pycom13g10480

4-coumarate--CoA ligase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0017194
Physical Location & Seq
Reverse (-)
7015820 .. 7018127
2308 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g10480.4

Sequence Viewer

Length: 1653 bp
ATGGGAACTTTTATGCAGAACTCAGCCCAAGAGGATGAACATATTTTCCGAAGCCGGTCCCCATCTGTCCGAGTACCTGATAACATCACATTGCCAGAATTTGTGCTCCAAAATGCAGAATTATACGCTGGAAATGTCGCGTTCGTGGAGGCTGTGACTGGAAAGAAAATTACGTACGGCGAGGTGGTTAGAGACACAAGGAGGTTTGCTAAAGCCTTAAGGTCTCTGGGCCTTAGAAAGGGGCAAGTGGTGGTGGTTGTTCTCCCAAATGTGGCAGAGTATGCCATTGTTGCCTTGGGAATAATGGCTGCTGGTGGTGTGTTTTCGGGTGCAAATCCGTCAGGGCACGCATCCGAAATTAAGAAGCAGGTTGACGTTGCCGATGCCAAGATAATTGTAACAAATGGTGCAACCTATGAAAAGGTAAAAGCTTTTGGGGTACCGGTAATTTTGCTTGGAGAAGAAGTTATTGAAGGTGCCATGAACTGGGAACACCTACTTGAAGCAGCAGACCGTGCGGGAAACAGAATTGATAAGGAGGAGCCGAAGCAGACTGATCGATGTGCTCTTCCCTTCTCATCAGGCACAATAGGATTGTCTAAGGGTGTAATGCTGACTCACCGAAACCTAGTTGCGAACCTCAGCTCCACGCTCTTTGGAGTAACGCCGGAAATGATTGGTAAAGTCACTGTGCTAGGCCTAATTCCATTCTTCCACATCTACGGGATCACTGGAATATGTTGTGCCACGCTCCGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATACGAACTTCGAACATTTCTTAACGCATTGATCTCACATGAGGTCACCTATGCGCCCATTGTGCCGCCAATCATTTTGAACTTAGTTAAGAGTCCAATTGTTGATGAATTTGATCTCAGCAAGCTCAAACTCCAGGCAATTATGACTGCAGCAGCTCCTCTTGCACCCGAAGTCCTCAACGCTTTCGAAAACAAGTTCCCCGGCGTCCAAGTCCAAGAGGCATACGGATTGACTGAGCATAGCTGCATCACACTCACTCATGCAGGACCAGGTCTTGTAGCAAAGAAAAACTCAGTAGGGTTTATCCTACCGAATTTGCTAGTCAAATTCATTGACCCCGACACCGGTCGTTCCCTACCGAGAAACACACCCGGTGAACTTTGCGTGTTGAGTCAATGTGTAATGCAAGGTTACTTTAACAACGAAGAGGACACTGCCCGGACTATCGACAAAGATGGATGGCTCCACACCGGAGATATTGGCTACATAGATGATGATGACAACGTCTTTATCGTTGACCGGATCAAGGAATTAATCAAGTACAAAGGTTTCCAAGTGGCTCCTGCTGAACTTGAGGCCATTCTTCTCAGTCATCCCTCGGTTGAAGATGTAGCAGTAGTCCCGCTGCCGGACGAGGAGGCAGGGGAGATTCCGGCAGCAAGTGTTGTGATCGCAGCTGGTGCAAAAGAGAGCGAGGAAGAGATAATCAACTACGTGGCCTCAAACGTTGCACAATACAAGAAAGTGAGAGTGGTGCACTTTGTGGACACAATTCCAAAATCGCCCTCTGGGAAGATCATGAGGAGGCTTATCAAGGAGAGCATGATAGAAAAGATCAAGGCATCCAAGCTCCACTGCCTTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001676 GO:0003674 GO:0003824 GO:0004467 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006082 GO:0006139 GO:0006163 GO:0006164 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009259 GO:0009260 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010584 GO:0010927 GO:0012505 GO:0015645 GO:0016043 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019752 GO:0022607 GO:0030198 GO:0031956 GO:0032501 GO:0032502 GO:0032787 GO:0032989 GO:0033865 GO:0033866 GO:0033875 GO:0034030 GO:0034032 GO:0034033 GO:0034641 GO:0034654 GO:0035337 GO:0035383 GO:0035384 GO:0043062 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046390 GO:0046483 GO:0046949 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0051186 GO:0051188 GO:0055086 GO:0071616 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0080110 GO:0085029 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901568 GO:1901570 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00130 ko00360 ko00940 ko01100 ko01110 map00130 map00360 map00940 map01100 map01110
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

551

Amino Acids

59.79

Weight (kDa)

6.01

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62940
fragaria_vesca FvH4_4g23640
malus_domestica MD13G1120400.v1.1
prunus_persica Prupe.1G224800_v2.0.a1
pyrus_communis pycom13g10480
rosa_chinensis RchiOBHm_Chr4g0430101
rosa_laevigata RLG00000007003
rosa_multiflora Rmu_ssc0000150.1_g000024
rosa_roxburghii Rroxscaffold_5G00371920
rosa_rugosa Rorug04G0240200
rosa_samantha Rh4AG297300 Rh4BG303700
rosa_wichuraiana Rw4G025780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 358
Acc65I GGTACC 1 cut(s) 439
AccB1I GGYRCC 2 cut(s) 439, 476
AccB7I CCANNNNNTGG 1 cut(s) 486
AccII CGCG 1 cut(s) 140
AciI CCGC 3 cut(s) 518, 851, 1409
AclI AACGTT 1 cut(s) 1512
AclWI GGATC 2 cut(s) 734, 1316
AcsI RAATTY 4 cut(s) 98, 893, 1099, 1112
AcyI GRCGYC 1 cut(s) 990
AdeI CACNNNGTG 2 cut(s) 1160, 1549
AfaI GTAC 4 cut(s) 75, 176, 441, 1328
AfiI CCNNNNNNNGG 7 cut(s) 238, 271, 486, 760, 1130, 1312, 1414
AflII CTTAAG 1 cut(s) 217
AgeI ACCGGT 2 cut(s) 442, 1130
AgsI TTSAA 4 cut(s) 473, 503, 865, 1391
AjnI CCWGG 2 cut(s) 918, 1054
AloI GAACNNNNNNTCC 4 cut(s) 30, 62, 629, 661
AluBI AGCT 7 cut(s) 431, 645, 910, 941, 1029, 1463, 1636
AluI AGCT 7 cut(s) 431, 645, 910, 941, 1029, 1463, 1636
Alw21I GWGCWC 3 cut(s) 108, 568, 1545
Alw26I GTCTC 2 cut(s) 186, 228
Alw44I GTGCAC 1 cut(s) 1541
AlwI GGATC 2 cut(s) 734, 1316
AoxI GGCC 4 cut(s) 229, 697, 1362, 1503
ApaLI GTGCAC 1 cut(s) 1541
ApeKI GCWGC 8 cut(s) 308, 506, 935, 938, 1029, 1411, 1442, 1460
ApoI RAATTY 4 cut(s) 98, 893, 1099, 1112
AseI ATTAAT 1 cut(s) 1319
AsiGI ACCGGT 2 cut(s) 442, 1130
Asp718I GGTACC 1 cut(s) 439
AspLEI GCGC 1 cut(s) 841
AspS9I GGNCC 3 cut(s) 57, 229, 1052
AsuC2I CCSGG 3 cut(s) 987, 1158, 1225
AsuHPI GGTGA 4 cut(s) 611, 787, 823, 1172
AsuII TTCGAA 2 cut(s) 796, 972
AvaII GGWCC 2 cut(s) 57, 1052
BaeGI GKGCMC 2 cut(s) 348, 1545
BanI GGYRCC 2 cut(s) 439, 476
Bbv12I GWGCWC 3 cut(s) 108, 568, 1545
BbvCI CCTCAGC 1 cut(s) 641
BbvI GCAGC 8 cut(s) 295, 518, 947, 950, 1016, 1398, 1454, 1472
BccI CCATC 4 cut(s) 70, 772, 1235, 1239
BceAI ACGGC 1 cut(s) 193
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BciT130I CCWGG 2 cut(s) 920, 1056
BcnI CCSGG 3 cut(s) 987, 1158, 1225
BcoDI GTCTC 2 cut(s) 186, 228
BfaI CTAG 3 cut(s) 629, 695, 1106
BfmI CTRYAG 1 cut(s) 933
BfrI CTTAAG 1 cut(s) 217
BfuAI ACCTGC 1 cut(s) 358
BisI GCNGC 9 cut(s) 309, 507, 851, 936, 939, 1030, 1412, 1443, 1461
BlsI GCNGC 9 cut(s) 310, 508, 852, 937, 940, 1031, 1413, 1444, 1462
Bme1390I CCNGG 5 cut(s) 920, 987, 1056, 1158, 1225
Bme18I GGWCC 2 cut(s) 57, 1052
BmgT120I GGNCC 3 cut(s) 57, 229, 1052
BmiI GGNNCC 6 cut(s) 59, 441, 478, 543, 1250, 1347
BmrFI CCNGG 5 cut(s) 920, 987, 1056, 1158, 1225
BmrI ACTGGG 1 cut(s) 496
BmsI GCATC 4 cut(s) 359, 373, 1041, 1637
BmuI ACTGGG 1 cut(s) 496
BoxI GACNNNNGTC 1 cut(s) 1131
BpmI CTGGAG 1 cut(s) 902
Bpu10I CCTNAGC 1 cut(s) 641
Bpu14I TTCGAA 2 cut(s) 796, 972
BpuEI CTTGAG 1 cut(s) 1379
BpuMI CCSGG 3 cut(s) 987, 1158, 1225
Bsa29I ATCGAT 1 cut(s) 559
BsaAI YACGTR 2 cut(s) 174, 1501
BsaHI GRCGYC 1 cut(s) 990
BsaI GGTCTC 1 cut(s) 228
BsaJI CCNNGG 3 cut(s) 294, 985, 1383
BsaWI WCCGGW 4 cut(s) 442, 1130, 1256, 1305
BsaXI ACNNNNNCTCC 4 cut(s) 450, 480, 629, 659
Bsc4I CCNNNNNNNGG 7 cut(s) 238, 271, 486, 760, 1130, 1312, 1414
Bse118I RCCGGY 3 cut(s) 54, 442, 1130
Bse1I ACTGG 3 cut(s) 163, 491, 736
Bse3DI GCAATG 1 cut(s) 89
BseBI CCWGG 2 cut(s) 920, 1056
BseCI ATCGAT 1 cut(s) 559
BseDI CCNNGG 3 cut(s) 294, 985, 1383
BseGI GGATG 5 cut(s) 40, 350, 1250, 1378, 1628
BseLI CCNNNNNNNGG 7 cut(s) 238, 271, 486, 760, 1130, 1312, 1414
BseMI GCAATG 1 cut(s) 89
BseMII CTCAG 6 cut(s) 36, 655, 916, 1011, 1092, 1387
BseNI ACTGG 3 cut(s) 163, 491, 736
BseRI GAGGAG 4 cut(s) 554, 933, 1436, 1603
BseSI GKGCMC 2 cut(s) 348, 1545
BseXI GCAGC 8 cut(s) 295, 518, 947, 950, 1016, 1398, 1454, 1472
Bsh1236I CGCG 1 cut(s) 140
Bsh1285I CGRYCG 1 cut(s) 1135
BshFI GGCC 4 cut(s) 231, 699, 1364, 1505
BshNI GGYRCC 2 cut(s) 439, 476
BshTI ACCGGT 2 cut(s) 442, 1130
BshVI ATCGAT 1 cut(s) 559
BsiEI CGRYCG 1 cut(s) 1135
BsiHKAI GWGCWC 3 cut(s) 108, 568, 1545
BsiWI CGTACG 1 cut(s) 174
BslFI GGGAC 2 cut(s) 43, 1391
BslI CCNNNNNNNGG 7 cut(s) 238, 271, 486, 760, 1130, 1312, 1414
BsmAI GTCTC 2 cut(s) 186, 228
BsmFI GGGAC 2 cut(s) 43, 1391
BsnI GGCC 4 cut(s) 231, 699, 1364, 1505
Bso31I GGTCTC 1 cut(s) 228
Bsp119I TTCGAA 2 cut(s) 796, 972
Bsp1286I GDGCHC 4 cut(s) 108, 348, 568, 1545
Bsp143I GATC 8 cut(s) 556, 726, 816, 898, 1308, 1455, 1581, 1620
BspACI CCGC 3 cut(s) 518, 851, 1409
BspANI GGCC 4 cut(s) 231, 699, 1364, 1505
BspCNI CTCAG 6 cut(s) 35, 654, 915, 1012, 1091, 1386
BspDI ATCGAT 1 cut(s) 559
BspFNI CGCG 1 cut(s) 140
BspHI TCATGA 1 cut(s) 1584
BspLI GGNNCC 6 cut(s) 59, 441, 478, 543, 1250, 1347
BspMAI CTGCAG 1 cut(s) 937
BspMI ACCTGC 1 cut(s) 358
BspPI GGATC 2 cut(s) 734, 1316
BspQI GCTCTTC 1 cut(s) 573
BspT104I TTCGAA 2 cut(s) 796, 972
BspT107I GGYRCC 2 cut(s) 439, 476
BspTI CTTAAG 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 228
BsrDI GCAATG 1 cut(s) 89
BsrFI RCCGGY 3 cut(s) 54, 442, 1130
BsrI ACTGG 3 cut(s) 163, 491, 736
BssAI RCCGGY 3 cut(s) 54, 442, 1130
BssECI CCNNGG 3 cut(s) 294, 985, 1383
BssMI GATC 8 cut(s) 556, 726, 816, 898, 1308, 1455, 1581, 1620
BssNI GRCGYC 1 cut(s) 990
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 2 cut(s) 920, 1056
Bst4CI ACNGT 2 cut(s) 515, 691
Bst6I CTCTTC 3 cut(s) 573, 1206, 1479
BstACI GRCGYC 1 cut(s) 990
BstAFI CTTAAG 1 cut(s) 217
BstAPI GCANNNNNTGC 3 cut(s) 281, 515, 1466
BstBAI YACGTR 2 cut(s) 174, 1501
BstBI TTCGAA 2 cut(s) 796, 972
BstC8I GCNNGC 2 cut(s) 348, 908
BstDEI CTNAG 9 cut(s) 22, 233, 600, 641, 868, 902, 1020, 1078, 1373
BstEII GGTNACC 1 cut(s) 829
BstENI CCTNNNNNAGG 1 cut(s) 236
BstF5I GGATG 5 cut(s) 40, 350, 1250, 1378, 1628
BstFNI CGCG 1 cut(s) 140
BstHHI GCGC 1 cut(s) 841
BstKTI GATC 8 cut(s) 559, 729, 819, 901, 1311, 1458, 1584, 1623
BstMAI GTCTC 2 cut(s) 186, 228
BstMBI GATC 8 cut(s) 556, 726, 816, 898, 1308, 1455, 1581, 1620
BstMCI CGRYCG 1 cut(s) 1135
BstMWI GCNNNNNNNGC 6 cut(s) 281, 290, 515, 847, 947, 1466
BstNI CCWGG 2 cut(s) 920, 1056
BstPAI GACNNNNGTC 1 cut(s) 1131
BstPI GGTNACC 1 cut(s) 829
BstSCI CCNGG 5 cut(s) 918, 985, 1054, 1156, 1223
BstSFI CTRYAG 1 cut(s) 933
BstSLI GKGCMC 2 cut(s) 348, 1545
BstSNI TACGTA 1 cut(s) 174
BstUI CGCG 1 cut(s) 140
BstV1I GCAGC 8 cut(s) 295, 518, 947, 950, 1016, 1398, 1454, 1472
Bsu15I ATCGAT 1 cut(s) 559
BsuRI GGCC 4 cut(s) 231, 699, 1364, 1505
BsuTUI ATCGAT 1 cut(s) 559
BtsCI GGATG 5 cut(s) 40, 350, 1250, 1378, 1628
BtsI GCAGTG 2 cut(s) 1218, 1639
BtsIMutI CAGTG 4 cut(s) 687, 729, 1218, 1639
BveI ACCTGC 1 cut(s) 358
Cac8I GCNNGC 2 cut(s) 348, 908
CciI TCATGA 1 cut(s) 1584
CfoI GCGC 1 cut(s) 841
Cfr10I RCCGGY 3 cut(s) 54, 442, 1130
Cfr13I GGNCC 3 cut(s) 57, 229, 1052
ClaI ATCGAT 1 cut(s) 559
CseI GACGC 1 cut(s) 979
CsiI ACCWGGT 1 cut(s) 1054
Csp6I GTAC 4 cut(s) 74, 175, 440, 1327
CspAI ACCGGT 2 cut(s) 442, 1130
CspCI CAANNNNNGTGG 2 cut(s) 637, 672
CviAII CATG 5 cut(s) 481, 824, 1046, 1585, 1609
CviQI GTAC 4 cut(s) 74, 175, 440, 1327
DdeI CTNAG 9 cut(s) 22, 233, 600, 641, 868, 902, 1020, 1078, 1373
DpnI GATC 8 cut(s) 558, 728, 818, 900, 1310, 1457, 1583, 1622
DpnII GATC 8 cut(s) 556, 726, 816, 898, 1308, 1455, 1581, 1620
DraIII CACNNNGTG 2 cut(s) 1160, 1549
Eam1104I CTCTTC 3 cut(s) 573, 1206, 1479
EarI CTCTTC 3 cut(s) 573, 1206, 1479
Eco105I TACGTA 1 cut(s) 174
Eco130I CCWWGG 1 cut(s) 294
Eco147I AGGCCT 1 cut(s) 699
Eco31I GGTCTC 1 cut(s) 228
Eco47I GGWCC 2 cut(s) 57, 1052
Eco91I GGTNACC 1 cut(s) 829
EcoNI CCTNNNNNAGG 1 cut(s) 236
EcoO65I GGTNACC 1 cut(s) 829
EcoRII CCWGG 2 cut(s) 918, 1054
EcoT14I CCWWGG 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 5 cut(s) 484, 827, 1049, 1588, 1612
FaqI GGGAC 2 cut(s) 43, 1391
FatI CATG 5 cut(s) 480, 823, 1045, 1584, 1608
FauI CCCGC 2 cut(s) 511, 1416
Fnu4HI GCNGC 9 cut(s) 309, 507, 851, 936, 939, 1030, 1412, 1443, 1461
FokI GGATG 5 cut(s) 47, 337, 1257, 1365, 1615
Fsp4HI GCNGC 9 cut(s) 309, 507, 851, 936, 939, 1030, 1412, 1443, 1461
FspBI CTAG 3 cut(s) 629, 695, 1106
GlaI GCGC 1 cut(s) 840
GluI GCNGC 9 cut(s) 309, 507, 851, 936, 939, 1030, 1412, 1443, 1461
GsuI CTGGAG 1 cut(s) 902
HaeIII GGCC 4 cut(s) 231, 699, 1364, 1505
HgaI GACGC 1 cut(s) 979
HhaI GCGC 1 cut(s) 841
Hin1I GRCGYC 1 cut(s) 990
Hin1II CATG 5 cut(s) 484, 827, 1049, 1588, 1612
Hin6I GCGC 1 cut(s) 839
HinP1I GCGC 1 cut(s) 839
HincII GTYRAC 2 cut(s) 373, 1303
HindII GTYRAC 2 cut(s) 373, 1303
HindIII AAGCTT 1 cut(s) 429
HinfI GANTC 4 cut(s) 616, 877, 1177, 1435
HphI GGTGA 4 cut(s) 611, 787, 823, 1172
Hpy166II GTNNAC 5 cut(s) 373, 1163, 1303, 1543, 1552
Hpy188I TCNGA 4 cut(s) 50, 71, 355, 755
Hpy188III TCNNGA 1 cut(s) 1585
Hpy8I GTNNAC 5 cut(s) 373, 1163, 1303, 1543, 1552
HpyAV CCTTC 2 cut(s) 467, 583
HpyCH4III ACNGT 2 cut(s) 515, 691
HpyCH4IV ACGT 5 cut(s) 173, 375, 1290, 1500, 1512
HpyF10VI GCNNNNNNNGC 6 cut(s) 281, 290, 515, 847, 947, 1466
HpyF3I CTNAG 9 cut(s) 22, 233, 600, 641, 868, 902, 1020, 1078, 1373
HpySE526I ACGT 5 cut(s) 173, 375, 1290, 1500, 1512
Hsp92I GRCGYC 1 cut(s) 990
Hsp92II CATG 5 cut(s) 484, 827, 1049, 1588, 1612
HspAI GCGC 1 cut(s) 839
KpnI GGTACC 1 cut(s) 443
Kzo9I GATC 8 cut(s) 556, 726, 816, 898, 1308, 1455, 1581, 1620
LguI GCTCTTC 1 cut(s) 573
LmnI GCTCC 8 cut(s) 111, 541, 650, 756, 946, 1254, 1351, 1641
Lsp1109I GCAGC 8 cut(s) 295, 518, 947, 950, 1016, 1398, 1454, 1472
LweI GCATC 4 cut(s) 359, 373, 1041, 1637
MabI ACCWGGT 1 cut(s) 1054
MaeI CTAG 3 cut(s) 629, 695, 1106
MaeII ACGT 5 cut(s) 173, 375, 1290, 1500, 1512
MaeIII GTNAC 6 cut(s) 154, 397, 661, 685, 829, 1196
MalI GATC 8 cut(s) 558, 728, 818, 900, 1310, 1457, 1583, 1622
MboI GATC 8 cut(s) 556, 726, 816, 898, 1308, 1455, 1581, 1620
MboII GAAGA 9 cut(s) 473, 560, 703, 795, 1223, 1361, 1403, 1496, 1591
MfeI CAATTG 1 cut(s) 882
MhlI GDGCHC 4 cut(s) 108, 348, 568, 1545
MlyI GAGTC 3 cut(s) 610, 886, 1186
MseI TTAA 7 cut(s) 218, 360, 807, 873, 1203, 1319, 1647
MspA1I CMGCKG 2 cut(s) 1411, 1463
MspCI CTTAAG 1 cut(s) 217
MspR9I CCNGG 5 cut(s) 920, 987, 1056, 1158, 1225
MunI CAATTG 1 cut(s) 882
MvaI CCWGG 2 cut(s) 920, 1056
MvnI CGCG 1 cut(s) 140
MwoI GCNNNNNNNGC 6 cut(s) 281, 290, 515, 847, 947, 1466
NciI CCSGG 3 cut(s) 987, 1158, 1225
NdeII GATC 8 cut(s) 556, 726, 816, 898, 1308, 1455, 1581, 1620
NlaIII CATG 5 cut(s) 484, 827, 1049, 1588, 1612
NlaIV GGNNCC 6 cut(s) 59, 441, 478, 543, 1250, 1347
NmuCI GTSAC 3 cut(s) 154, 685, 829
NspV TTCGAA 2 cut(s) 796, 972
PagI TCATGA 1 cut(s) 1584
PceI AGGCCT 1 cut(s) 699
PciSI GCTCTTC 1 cut(s) 573
PcsI WCGNNNNNNNCGW 1 cut(s) 1296
PfeI GAWTC 1 cut(s) 1435
Pfl23II CGTACG 1 cut(s) 174
PflFI GACNNNGTC 2 cut(s) 1056, 1289
PflMI CCANNNNNTGG 1 cut(s) 486
PinAI ACCGGT 2 cut(s) 442, 1130
PkrI GCNGC 9 cut(s) 310, 508, 852, 937, 940, 1031, 1413, 1444, 1462
PleI GAGTC 3 cut(s) 610, 885, 1185
PpsI GAGTC 3 cut(s) 610, 885, 1185
Ppu21I YACGTR 2 cut(s) 174, 1501
PshAI GACNNNNGTC 1 cut(s) 1131
PshBI ATTAAT 1 cut(s) 1319
Psp1406I AACGTT 1 cut(s) 1512
Psp6I CCWGG 2 cut(s) 918, 1054
PspEI GGTNACC 1 cut(s) 829
PspGI CCWGG 2 cut(s) 918, 1054
PspLI CGTACG 1 cut(s) 174
PspN4I GGNNCC 6 cut(s) 59, 441, 478, 543, 1250, 1347
PspPI GGNCC 3 cut(s) 57, 229, 1052
PstI CTGCAG 1 cut(s) 937
PsyI GACNNNGTC 2 cut(s) 1056, 1289
PvuII CAGCTG 1 cut(s) 1463
RsaI GTAC 4 cut(s) 75, 176, 441, 1328
RsaNI GTAC 4 cut(s) 74, 175, 440, 1327
SapI GCTCTTC 1 cut(s) 573
SaqAI TTAA 7 cut(s) 218, 360, 807, 873, 1203, 1319, 1647
SatI GCNGC 9 cut(s) 309, 507, 851, 936, 939, 1030, 1412, 1443, 1461
Sau3AI GATC 8 cut(s) 556, 726, 816, 898, 1308, 1455, 1581, 1620
Sau96I GGNCC 3 cut(s) 57, 229, 1052
SchI GAGTC 3 cut(s) 610, 886, 1186
ScrFI CCNGG 5 cut(s) 920, 987, 1056, 1158, 1225
SduI GDGCHC 4 cut(s) 108, 348, 568, 1545
SexAI ACCWGGT 1 cut(s) 1054
SfaNI GCATC 4 cut(s) 359, 373, 1041, 1637
SfcI CTRYAG 1 cut(s) 933
SfuI TTCGAA 2 cut(s) 796, 972
SinI GGWCC 2 cut(s) 57, 1052
SmlI CTYRAG 2 cut(s) 217, 1358
SmoI CTYRAG 2 cut(s) 217, 1358
SnaBI TACGTA 1 cut(s) 174
SseBI AGGCCT 1 cut(s) 699
SsiI CCGC 3 cut(s) 518, 851, 1409
SspMI CTAG 3 cut(s) 629, 695, 1106
StuI AGGCCT 1 cut(s) 699
StyD4I CCNGG 5 cut(s) 918, 985, 1054, 1156, 1223
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 2 cut(s) 515, 691
TaiI ACGT 5 cut(s) 176, 378, 1293, 1503, 1515
TaqI TCGA 4 cut(s) 559, 796, 972, 1233
TatI WGTACW 1 cut(s) 1326
TauI GCSGC 1 cut(s) 853
TfiI GAWTC 1 cut(s) 1435
Tru1I TTAA 7 cut(s) 218, 360, 807, 873, 1203, 1319, 1647
Tru9I TTAA 7 cut(s) 218, 360, 807, 873, 1203, 1319, 1647
TscAI CASTG 4 cut(s) 694, 736, 1225, 1646
TseFI GTSAC 3 cut(s) 154, 685, 829
TseI GCWGC 8 cut(s) 308, 506, 935, 938, 1029, 1411, 1442, 1460
Tsp45I GTSAC 3 cut(s) 154, 685, 829
TspDTI ATGAA 5 cut(s) 51, 432, 497, 906, 1105
TspGWI ACGGA 2 cut(s) 327, 1026
TspRI CASTG 4 cut(s) 694, 736, 1225, 1646
Tth111I GACNNNGTC 2 cut(s) 1056, 1289
Van91I CCANNNNNTGG 1 cut(s) 486
Vha464I CTTAAG 1 cut(s) 217
VneI GTGCAC 1 cut(s) 1541
VpaK11BI GGWCC 2 cut(s) 57, 1052
VspI ATTAAT 1 cut(s) 1319
XagI CCTNNNNNAGG 1 cut(s) 236
XapI RAATTY 4 cut(s) 98, 893, 1099, 1112
XcmI CCANNNNNNNNNTGG 1 cut(s) 292
XspI CTAG 3 cut(s) 629, 695, 1106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.