MD13G1120400.v1.1

4-coumarate--CoA ligase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0017194
Physical Location & Seq
Reverse (-)
8853168 .. 8855750
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1120400.v1.1.491

Sequence Viewer

Length: 1725 bp
ATGGTAAATCTCATACCTTCACTCGTTTCAACCTCCCTTTGGCTTCCATTAGCTCCCTTTCACATATTCATCATGGGAACTTTTGTGCAGAACTCAGCCCAAGAGGATGAACATATTTTCGGAAGCCGGTCCCCATCTGTCCGAGTACCTGATAACATCACATTGCCAGAATTTGTGCTCCAAAATGCAGAATTATACGCTGGAAATGTCGCGTTCGTGGAGGCTGTGACTGGAAAGAAAATTACGTACGGCGAGGTGGTTAGAGACACAAGGAGGTTTGCTAAAGCCTTAAGGTCTCTTGGCCTTAGAAAGGGGCAAGTGGTGGTGGTTGTTCTCCCAAATGTGGCAGAGTATGCCATTGTTGCCTTGGGAATAATGGCTGCTGGTGGTGTGTTTTCGGGTGCAAATCCATCGGGGCACGCATCCGAAATTAAGAAGCAAGTTGAGGTTGCTGATGCCAAGATAATTGTAACAAATGGTGCAACCTATGAAAAGGTAAAATCTTTTGGGGTGCCGGTAATTTTGCTTGGAGAAGAGGTTATTGAAGGTGCCATGAACTGGGAACACCTACTTGAAGCAGCAGACCGTGCGGGAAACAGAATTAATGAGGAGGAGCTGAAGCAGACTGATCTATGTGCCCTTCCCTTCTCATCAGGCACAACAGGATTGTCTAAGGGTGTAATGCTGACTCACCGAAACCTAGTAGCGAACCTCAGCTCCACGCTCTTTGGAGTAACGCCGGAAATGATTGGTAAAGTCACCGTGCTAGGCCTAATTCCGTTCTTCCACATCTACGGGATCACTGGAATATGTTGTGCCACGCTCCGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATACGAACTTCGGACATTTCTTAACGCATTGATCTCACATGAGGTTACCTATGCGCCCATTGTGCCGCCAATCATTTTGAACTTGGTTAAGAATCCAATTGTTGATGAATTTGATCTCAGCAAGCTCAAACTCCAGGCAATTATGACTGCAGCAGCTCCTCTTGCACCCGAAGTCCTCAACGCTTTCGAAAACAAGTTCCCCGGCGTCCAAGTCCAAGAGGCATATGGATTGACTGAGCATAGCTGCATCACACTCACTCATGCAGGACCAGGTCTTGTAGCAAAGAAAAACTCAGTAGGGTTTATTCTTCCAAATTTGCTAGTCAAATTCATTGACCCGGACACCGGTCGTTCCCTACCGAGAAATACACCCGGTGAACTTTGCGTGTTGAGTCAATGTGTAATGCAAGGTTACTTTAACAACGAAGAGGAGACTGCCCGGACTATCGACAAAGATGGATGGCTTCACACCGGAGATATTGGCTGCATAGATGATGATGACAACGTCTTTATCGTTGACCGGATCAAGGAATTAATCAAGTACAAAGGTTTCCAAGTGGCTCCTGCTGAACTTGAGGCCATTCTTCTGAGTCATCCCTCGGTTGAAGATGTAGCAGTAGTCCCGCTGCCGGACGAGGAGGCAGGGGAGATTCCGGCAGCAAGTGTTGTGATCGCAGCTGGTACAAAAGAGAGCGAGGAAGAGATAATCAACTACGTGGCCTCAAACGTTGCACAATACAAGAAAGTGAGAGTGGTGCACTTTGTGGACACAATTCCAAAATCGCCCTCGGGGAAGATCATGAGGAGGCTTATCAAAGAGAGCATGATGGAAAAGATCAAGGCATCCAAGCTCCACTGCCTTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001676 GO:0003674 GO:0003824 GO:0004467 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006082 GO:0006139 GO:0006163 GO:0006164 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009259 GO:0009260 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010584 GO:0010927 GO:0012505 GO:0015645 GO:0016043 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019752 GO:0022607 GO:0030198 GO:0031956 GO:0032501 GO:0032502 GO:0032787 GO:0032989 GO:0033865 GO:0033866 GO:0033875 GO:0034030 GO:0034032 GO:0034033 GO:0034641 GO:0034654 GO:0035337 GO:0035383 GO:0035384 GO:0043062 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046390 GO:0046483 GO:0046949 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0051186 GO:0051188 GO:0055086 GO:0071616 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0080110 GO:0085029 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901568 GO:1901570 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00130 ko00360 ko00940 ko01100 ko01110 map00130 map00360 map00940 map01100 map01110
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

575

Amino Acids

62.37

Weight (kDa)

5.8

Isoelectric Point (pI)

34.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 62 - 425 1.3e-77 AMP-binding enzyme
AMP-binding_C PF13193 476 - 551 6.3e-19 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62940
fragaria_vesca FvH4_4g23640
malus_domestica MD13G1120400.v1.1
prunus_persica Prupe.1G224800_v2.0.a1
pyrus_communis pycom13g10480
rosa_chinensis RchiOBHm_Chr4g0430101
rosa_laevigata RLG00000007003
rosa_multiflora Rmu_ssc0000150.1_g000024
rosa_roxburghii Rroxscaffold_5G00371920
rosa_rugosa Rorug04G0240200
rosa_samantha Rh4AG297300 Rh4BG303700
rosa_wichuraiana Rw4G025780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 511, 548
AccB7I CCANNNNNTGG 1 cut(s) 558
AccII CGCG 1 cut(s) 212
AciI CCGC 3 cut(s) 590, 923, 1481
AclI AACGTT 1 cut(s) 1584
AclWI GGATC 2 cut(s) 806, 1388
AcsI RAATTY 4 cut(s) 170, 965, 1171, 1184
AcuI CTGAAG 1 cut(s) 638
AcyI GRCGYC 1 cut(s) 1062
AdeI CACNNNGTG 2 cut(s) 1232, 1621
AfaI GTAC 4 cut(s) 147, 248, 1400, 1540
AfiI CCNNNNNNNGG 8 cut(s) 39, 310, 343, 558, 832, 1202, 1384, 1486
AflII CTTAAG 1 cut(s) 289
AgeI ACCGGT 1 cut(s) 1202
AgsI TTSAA 5 cut(s) 30, 545, 575, 937, 1463
AjnI CCWGG 2 cut(s) 990, 1126
AloI GAACNNNNNNTCC 2 cut(s) 701, 733
AluBI AGCT 8 cut(s) 53, 616, 717, 982, 1013, 1101, 1535, 1708
AluI AGCT 8 cut(s) 53, 616, 717, 982, 1013, 1101, 1535, 1708
Alw21I GWGCWC 2 cut(s) 180, 1617
Alw26I GTCTC 3 cut(s) 258, 300, 1283
Alw44I GTGCAC 1 cut(s) 1613
AlwI GGATC 2 cut(s) 806, 1388
Ama87I CYCGRG 1 cut(s) 1645
AoxI GGCC 4 cut(s) 301, 769, 1434, 1575
ApaLI GTGCAC 1 cut(s) 1613
ApeKI GCWGC 9 cut(s) 380, 578, 1007, 1010, 1101, 1341, 1483, 1514, 1532
ApoI RAATTY 4 cut(s) 170, 965, 1171, 1184
AseI ATTAAT 2 cut(s) 603, 1391
AsiGI ACCGGT 1 cut(s) 1202
AspLEI GCGC 1 cut(s) 913
AspS9I GGNCC 2 cut(s) 129, 1124
AsuC2I CCSGG 4 cut(s) 1059, 1196, 1230, 1297
AsuHPI GGTGA 4 cut(s) 683, 751, 859, 1244
AsuII TTCGAA 1 cut(s) 1044
AvaI CYCGRG 1 cut(s) 1645
AvaII GGWCC 2 cut(s) 129, 1124
BaeGI GKGCMC 3 cut(s) 420, 640, 1617
BanI GGYRCC 2 cut(s) 511, 548
Bbv12I GWGCWC 2 cut(s) 180, 1617
BbvCI CCTCAGC 1 cut(s) 713
BbvI GCAGC 9 cut(s) 367, 590, 1019, 1022, 1088, 1328, 1470, 1526, 1544
BccI CCATC 6 cut(s) 142, 418, 844, 1307, 1311, 1678
BceAI ACGGC 1 cut(s) 265
BcgI CGANNNNNNTGC 2 cut(s) 393, 427
BciT130I CCWGG 2 cut(s) 992, 1128
BcnI CCSGG 4 cut(s) 1059, 1196, 1230, 1297
BcoDI GTCTC 3 cut(s) 258, 300, 1283
BfaI CTAG 3 cut(s) 701, 767, 1178
BfmI CTRYAG 1 cut(s) 1005
BfrI CTTAAG 1 cut(s) 289
Bme1390I CCNGG 6 cut(s) 992, 1059, 1128, 1196, 1230, 1297
Bme18I GGWCC 2 cut(s) 129, 1124
BmeT110I CYCGRG 1 cut(s) 1645
BmgT120I GGNCC 2 cut(s) 129, 1124
BmiI GGNNCC 4 cut(s) 131, 513, 550, 1419
BmrFI CCNGG 6 cut(s) 992, 1059, 1128, 1196, 1230, 1297
BmrI ACTGGG 1 cut(s) 568
BmsI GCATC 4 cut(s) 431, 445, 1113, 1709
BmuI ACTGGG 1 cut(s) 568
BoxI GACNNNNGTC 1 cut(s) 1203
BpmI CTGGAG 1 cut(s) 974
Bpu10I CCTNAGC 1 cut(s) 713
Bpu14I TTCGAA 1 cut(s) 1044
BpuEI CTTGAG 1 cut(s) 1451
BpuMI CCSGG 4 cut(s) 1059, 1196, 1230, 1297
BsaAI YACGTR 2 cut(s) 246, 1573
BsaHI GRCGYC 1 cut(s) 1062
BsaI GGTCTC 1 cut(s) 300
BsaJI CCNNGG 4 cut(s) 366, 1057, 1455, 1644
BsaWI WCCGGW 3 cut(s) 1202, 1328, 1377
BsaXI ACNNNNNCTCC 4 cut(s) 522, 552, 701, 731
Bsc4I CCNNNNNNNGG 8 cut(s) 39, 310, 343, 558, 832, 1202, 1384, 1486
Bse118I RCCGGY 3 cut(s) 126, 514, 1202
Bse1I ACTGG 3 cut(s) 235, 563, 808
Bse3DI GCAATG 1 cut(s) 161
BseBI CCWGG 2 cut(s) 992, 1128
BseDI CCNNGG 4 cut(s) 366, 1057, 1455, 1644
BseGI GGATG 5 cut(s) 112, 422, 1322, 1450, 1700
BseLI CCNNNNNNNGG 8 cut(s) 39, 310, 343, 558, 832, 1202, 1384, 1486
BseMI GCAATG 1 cut(s) 161
BseMII CTCAG 6 cut(s) 108, 727, 988, 1083, 1164, 1436
BseNI ACTGG 3 cut(s) 235, 563, 808
BseRI GAGGAG 6 cut(s) 623, 626, 1005, 1301, 1508, 1675
BseSI GKGCMC 3 cut(s) 420, 640, 1617
BseXI GCAGC 9 cut(s) 367, 590, 1019, 1022, 1088, 1328, 1470, 1526, 1544
BsgI GTGCAG 1 cut(s) 107
Bsh1236I CGCG 1 cut(s) 212
Bsh1285I CGRYCG 1 cut(s) 1207
BshFI GGCC 4 cut(s) 303, 771, 1436, 1577
BshNI GGYRCC 2 cut(s) 511, 548
BshTI ACCGGT 1 cut(s) 1202
BsiEI CGRYCG 1 cut(s) 1207
BsiHKAI GWGCWC 2 cut(s) 180, 1617
BsiHKCI CYCGRG 1 cut(s) 1645
BsiWI CGTACG 1 cut(s) 246
BslFI GGGAC 2 cut(s) 115, 1463
BslI CCNNNNNNNGG 8 cut(s) 39, 310, 343, 558, 832, 1202, 1384, 1486
BsmAI GTCTC 3 cut(s) 258, 300, 1283
BsmFI GGGAC 2 cut(s) 115, 1463
BsnI GGCC 4 cut(s) 303, 771, 1436, 1577
Bso31I GGTCTC 1 cut(s) 300
BsoBI CYCGRG 1 cut(s) 1645
Bsp119I TTCGAA 1 cut(s) 1044
Bsp1286I GDGCHC 4 cut(s) 180, 420, 640, 1617
Bsp143I GATC 8 cut(s) 628, 798, 888, 970, 1380, 1527, 1653, 1692
BspACI CCGC 3 cut(s) 590, 923, 1481
BspANI GGCC 4 cut(s) 303, 771, 1436, 1577
BspCNI CTCAG 6 cut(s) 107, 726, 987, 1084, 1163, 1437
BspFNI CGCG 1 cut(s) 212
BspHI TCATGA 1 cut(s) 1656
BspLI GGNNCC 4 cut(s) 131, 513, 550, 1419
BspMAI CTGCAG 1 cut(s) 1009
BspPI GGATC 2 cut(s) 806, 1388
BspT104I TTCGAA 1 cut(s) 1044
BspT107I GGYRCC 2 cut(s) 511, 548
BspTI CTTAAG 1 cut(s) 289
BspTNI GGTCTC 1 cut(s) 300
BsrDI GCAATG 1 cut(s) 161
BsrFI RCCGGY 3 cut(s) 126, 514, 1202
BsrI ACTGG 3 cut(s) 235, 563, 808
BssAI RCCGGY 3 cut(s) 126, 514, 1202
BssECI CCNNGG 4 cut(s) 366, 1057, 1455, 1644
BssMI GATC 8 cut(s) 628, 798, 888, 970, 1380, 1527, 1653, 1692
BssNI GRCGYC 1 cut(s) 1062
BssT1I CCWWGG 1 cut(s) 366
Bst2UI CCWGG 2 cut(s) 992, 1128
Bst4CI ACNGT 2 cut(s) 587, 763
Bst6I CTCTTC 3 cut(s) 528, 1278, 1551
BstACI GRCGYC 1 cut(s) 1062
BstAFI CTTAAG 1 cut(s) 289
BstAPI GCANNNNNTGC 2 cut(s) 353, 587
BstBAI YACGTR 2 cut(s) 246, 1573
BstBI TTCGAA 1 cut(s) 1044
BstC8I GCNNGC 2 cut(s) 420, 980
BstDEI CTNAG 8 cut(s) 94, 305, 672, 713, 974, 1092, 1150, 1445
BstEII GGTNACC 1 cut(s) 901
BstENI CCTNNNNNAGG 1 cut(s) 308
BstF5I GGATG 5 cut(s) 112, 422, 1322, 1450, 1700
BstFNI CGCG 1 cut(s) 212
BstHHI GCGC 1 cut(s) 913
BstKTI GATC 8 cut(s) 631, 801, 891, 973, 1383, 1530, 1656, 1695
BstMAI GTCTC 3 cut(s) 258, 300, 1283
BstMBI GATC 8 cut(s) 628, 798, 888, 970, 1380, 1527, 1653, 1692
BstMCI CGRYCG 1 cut(s) 1207
BstMWI GCNNNNNNNGC 5 cut(s) 353, 362, 587, 919, 1019
BstNI CCWGG 2 cut(s) 992, 1128
BstPAI GACNNNNGTC 1 cut(s) 1203
BstPI GGTNACC 1 cut(s) 901
BstSCI CCNGG 6 cut(s) 990, 1057, 1126, 1194, 1228, 1295
BstSFI CTRYAG 1 cut(s) 1005
BstSLI GKGCMC 3 cut(s) 420, 640, 1617
BstSNI TACGTA 1 cut(s) 246
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 9 cut(s) 367, 590, 1019, 1022, 1088, 1328, 1470, 1526, 1544
BsuRI GGCC 4 cut(s) 303, 771, 1436, 1577
BtsCI GGATG 5 cut(s) 112, 422, 1322, 1450, 1700
BtsI GCAGTG 1 cut(s) 1711
BtsIMutI CAGTG 2 cut(s) 801, 1711
Cac8I GCNNGC 2 cut(s) 420, 980
CciI TCATGA 1 cut(s) 1656
CfoI GCGC 1 cut(s) 913
Cfr10I RCCGGY 3 cut(s) 126, 514, 1202
Cfr13I GGNCC 2 cut(s) 129, 1124
CseI GACGC 1 cut(s) 1051
CsiI ACCWGGT 1 cut(s) 1126
Csp6I GTAC 4 cut(s) 146, 247, 1399, 1539
CspAI ACCGGT 1 cut(s) 1202
CspCI CAANNNNNGTGG 2 cut(s) 709, 744
CviAII CATG 6 cut(s) 73, 553, 896, 1118, 1657, 1681
CviQI GTAC 4 cut(s) 146, 247, 1399, 1539
DdeI CTNAG 8 cut(s) 94, 305, 672, 713, 974, 1092, 1150, 1445
DpnI GATC 8 cut(s) 630, 800, 890, 972, 1382, 1529, 1655, 1694
DpnII GATC 8 cut(s) 628, 798, 888, 970, 1380, 1527, 1653, 1692
DraIII CACNNNGTG 2 cut(s) 1232, 1621
Eam1104I CTCTTC 3 cut(s) 528, 1278, 1551
EarI CTCTTC 3 cut(s) 528, 1278, 1551
Eco105I TACGTA 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 366
Eco147I AGGCCT 1 cut(s) 771
Eco31I GGTCTC 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 129, 1124
Eco57I CTGAAG 1 cut(s) 638
Eco88I CYCGRG 1 cut(s) 1645
Eco91I GGTNACC 1 cut(s) 901
EcoNI CCTNNNNNAGG 1 cut(s) 308
EcoO65I GGTNACC 1 cut(s) 901
EcoRII CCWGG 2 cut(s) 990, 1126
EcoT14I CCWWGG 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 6 cut(s) 76, 556, 899, 1121, 1660, 1684
FaqI GGGAC 2 cut(s) 115, 1463
FatI CATG 6 cut(s) 72, 552, 895, 1117, 1656, 1680
FauI CCCGC 2 cut(s) 583, 1488
FauNDI CATATG 1 cut(s) 1081
FokI GGATG 5 cut(s) 119, 409, 1329, 1437, 1687
FspBI CTAG 3 cut(s) 701, 767, 1178
GlaI GCGC 1 cut(s) 912
GsuI CTGGAG 1 cut(s) 974
HaeIII GGCC 4 cut(s) 303, 771, 1436, 1577
HgaI GACGC 1 cut(s) 1051
HhaI GCGC 1 cut(s) 913
Hin1I GRCGYC 1 cut(s) 1062
Hin1II CATG 6 cut(s) 76, 556, 899, 1121, 1660, 1684
Hin6I GCGC 1 cut(s) 911
HinP1I GCGC 1 cut(s) 911
HincII GTYRAC 1 cut(s) 1375
HindII GTYRAC 1 cut(s) 1375
HinfI GANTC 5 cut(s) 688, 949, 1249, 1447, 1507
HphI GGTGA 4 cut(s) 683, 751, 859, 1244
Hpy166II GTNNAC 4 cut(s) 1235, 1375, 1615, 1624
Hpy188I TCNGA 6 cut(s) 122, 143, 427, 827, 870, 1446
Hpy188III TCNNGA 1 cut(s) 1657
Hpy8I GTNNAC 4 cut(s) 1235, 1375, 1615, 1624
HpyAV CCTTC 4 cut(s) 27, 539, 650, 655
HpyCH4III ACNGT 2 cut(s) 587, 763
HpyCH4IV ACGT 4 cut(s) 245, 1362, 1572, 1584
HpyF10VI GCNNNNNNNGC 5 cut(s) 353, 362, 587, 919, 1019
HpyF3I CTNAG 8 cut(s) 94, 305, 672, 713, 974, 1092, 1150, 1445
HpySE526I ACGT 4 cut(s) 245, 1362, 1572, 1584
Hsp92I GRCGYC 1 cut(s) 1062
Hsp92II CATG 6 cut(s) 76, 556, 899, 1121, 1660, 1684
HspAI GCGC 1 cut(s) 911
Kzo9I GATC 8 cut(s) 628, 798, 888, 970, 1380, 1527, 1653, 1692
LmnI GCTCC 8 cut(s) 58, 183, 613, 722, 828, 1018, 1423, 1713
Lsp1109I GCAGC 9 cut(s) 367, 590, 1019, 1022, 1088, 1328, 1470, 1526, 1544
LweI GCATC 4 cut(s) 431, 445, 1113, 1709
MabI ACCWGGT 1 cut(s) 1126
MaeI CTAG 3 cut(s) 701, 767, 1178
MaeII ACGT 4 cut(s) 245, 1362, 1572, 1584
MaeIII GTNAC 6 cut(s) 226, 469, 733, 757, 901, 1268
MalI GATC 8 cut(s) 630, 800, 890, 972, 1382, 1529, 1655, 1694
MboI GATC 8 cut(s) 628, 798, 888, 970, 1380, 1527, 1653, 1692
MboII GAAGA 9 cut(s) 545, 775, 867, 1157, 1295, 1433, 1475, 1568, 1663
MfeI CAATTG 1 cut(s) 954
MhlI GDGCHC 4 cut(s) 180, 420, 640, 1617
MlyI GAGTC 3 cut(s) 682, 1258, 1456
MseI TTAA 8 cut(s) 290, 432, 603, 879, 945, 1275, 1391, 1719
MspA1I CMGCKG 2 cut(s) 1483, 1535
MspCI CTTAAG 1 cut(s) 289
MspR9I CCNGG 6 cut(s) 992, 1059, 1128, 1196, 1230, 1297
MunI CAATTG 1 cut(s) 954
MvaI CCWGG 2 cut(s) 992, 1128
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 5 cut(s) 353, 362, 587, 919, 1019
NciI CCSGG 4 cut(s) 1059, 1196, 1230, 1297
NdeI CATATG 1 cut(s) 1081
NdeII GATC 8 cut(s) 628, 798, 888, 970, 1380, 1527, 1653, 1692
NlaIII CATG 6 cut(s) 76, 556, 899, 1121, 1660, 1684
NlaIV GGNNCC 4 cut(s) 131, 513, 550, 1419
NmuCI GTSAC 2 cut(s) 226, 757
NspV TTCGAA 1 cut(s) 1044
PagI TCATGA 1 cut(s) 1656
PceI AGGCCT 1 cut(s) 771
PcsI WCGNNNNNNNCGW 1 cut(s) 1368
PfeI GAWTC 2 cut(s) 949, 1507
Pfl23II CGTACG 1 cut(s) 246
PflFI GACNNNGTC 2 cut(s) 1128, 1361
PflMI CCANNNNNTGG 1 cut(s) 558
PinAI ACCGGT 1 cut(s) 1202
PleI GAGTC 3 cut(s) 682, 1257, 1455
PpsI GAGTC 3 cut(s) 682, 1257, 1455
Ppu21I YACGTR 2 cut(s) 246, 1573
PshAI GACNNNNGTC 1 cut(s) 1203
PshBI ATTAAT 2 cut(s) 603, 1391
Psp1406I AACGTT 1 cut(s) 1584
Psp6I CCWGG 2 cut(s) 990, 1126
PspEI GGTNACC 1 cut(s) 901
PspGI CCWGG 2 cut(s) 990, 1126
PspLI CGTACG 1 cut(s) 246
PspN4I GGNNCC 4 cut(s) 131, 513, 550, 1419
PspPI GGNCC 2 cut(s) 129, 1124
PstI CTGCAG 1 cut(s) 1009
PsyI GACNNNGTC 2 cut(s) 1128, 1361
PvuII CAGCTG 1 cut(s) 1535
RsaI GTAC 4 cut(s) 147, 248, 1400, 1540
RsaNI GTAC 4 cut(s) 146, 247, 1399, 1539
SaqAI TTAA 8 cut(s) 290, 432, 603, 879, 945, 1275, 1391, 1719
Sau3AI GATC 8 cut(s) 628, 798, 888, 970, 1380, 1527, 1653, 1692
Sau96I GGNCC 2 cut(s) 129, 1124
SchI GAGTC 3 cut(s) 682, 1258, 1456
ScrFI CCNGG 6 cut(s) 992, 1059, 1128, 1196, 1230, 1297
SduI GDGCHC 4 cut(s) 180, 420, 640, 1617
SexAI ACCWGGT 1 cut(s) 1126
SfaNI GCATC 4 cut(s) 431, 445, 1113, 1709
SfcI CTRYAG 1 cut(s) 1005
SfuI TTCGAA 1 cut(s) 1044
SinI GGWCC 2 cut(s) 129, 1124
SmlI CTYRAG 2 cut(s) 289, 1430
SmoI CTYRAG 2 cut(s) 289, 1430
SnaBI TACGTA 1 cut(s) 246
SseBI AGGCCT 1 cut(s) 771
SsiI CCGC 3 cut(s) 590, 923, 1481
SspMI CTAG 3 cut(s) 701, 767, 1178
StuI AGGCCT 1 cut(s) 771
StyD4I CCNGG 6 cut(s) 990, 1057, 1126, 1194, 1228, 1295
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 2 cut(s) 587, 763
TaiI ACGT 4 cut(s) 248, 1365, 1575, 1587
TaqI TCGA 2 cut(s) 1044, 1305
TatI WGTACW 1 cut(s) 1398
TauI GCSGC 1 cut(s) 925
TfiI GAWTC 2 cut(s) 949, 1507
Tru1I TTAA 8 cut(s) 290, 432, 603, 879, 945, 1275, 1391, 1719
Tru9I TTAA 8 cut(s) 290, 432, 603, 879, 945, 1275, 1391, 1719
TscAI CASTG 2 cut(s) 808, 1718
TseFI GTSAC 2 cut(s) 226, 757
TseI GCWGC 9 cut(s) 380, 578, 1007, 1010, 1101, 1341, 1483, 1514, 1532
Tsp45I GTSAC 2 cut(s) 226, 757
TspDTI ATGAA 6 cut(s) 58, 123, 504, 569, 978, 1177
TspGWI ACGGA 1 cut(s) 768
TspRI CASTG 2 cut(s) 808, 1718
Tth111I GACNNNGTC 2 cut(s) 1128, 1361
Van91I CCANNNNNTGG 1 cut(s) 558
Vha464I CTTAAG 1 cut(s) 289
VneI GTGCAC 1 cut(s) 1613
VpaK11BI GGWCC 2 cut(s) 129, 1124
VspI ATTAAT 2 cut(s) 603, 1391
XagI CCTNNNNNAGG 1 cut(s) 308
XapI RAATTY 4 cut(s) 170, 965, 1171, 1184
XcmI CCANNNNNNNNNTGG 2 cut(s) 364, 1079
XspI CTAG 3 cut(s) 701, 767, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.