Prupe.1G224800_v2.0.a1

4-coumarate--CoA ligase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0017194
Physical Location & Seq
Forward (+)
23860817 .. 23863401
2585 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G224800.1

Sequence Viewer

Length: 1647 bp
ATGGGAACTTTTGTGCACAACTCAGCCCAAGATGAGGAACATATTTTCCGAAGCCGAGCACCGGCTGTCCAAGTACCTGATAATGTCACATTGCCAGAATTTGTGCTTCAGGATGCAGAATTATATGCTGATAATGTTGCATTCGTGGAGGCGGTGACCGGAAAGGAAATTACTTATGGGGAGGTGGTGAGAGACACTAGGAGATTTTCCAAGGCCTTAAGGTCTCTTGGCCTTAGAAAGGGGCATGTGGTGGTGGTTGTTCTCCCAAATGTTGCAGAGTATGCCATAGTTGCTTTGGGAATAATGGCTGCTGGTGGGGTGTTTTCGGGAGCAAATCCATCAGGGCATGCATCTGAAATTAAGAAGCAGGTGGAGGTAGCTGATGCCAAGATCATTGTAACAAATGGTGCAAGCTATGAAAAGGTAAAAGGCTTTGGGGTACCAGTAATTGTGCTTGGTGAAGAAGTTATTGAAGGTGCTATGAATTGGAACAACCTGCTTGAAGCAGCAGACCGTGCCGGAAACAAAATCAATAAGGAGGAATTGAAGCAGTCTGATCTATGTGCCCTTCCCTTCTCATCAGGCACAACAGGACTGTCGAAGGGTGTAATGCTGACTCATCGAAACCTAGTAGCCAACCTCAGCTCCACACTCTTTGGAGTAACGCCGGAAATGATTGGCAAAGTCACTGTCCTAGGCCTAATTCCATTCTTTCACATCTATGGGATCACTGGAATATGTTGCGCGACGCTTCGAAACAAGGGGAAAGTGGTGGTGATGGGAAGATTCGAACTTCGGACATTTCTTAATGCATTGGTCTCACAAGAGGTCACATATGCGCCCATTGTGCCACCAATCATTTTGAACTTGGTTAAGAATCCAATAGTTGATGAGTTTGATCTGAGCAAGCTCAAACTCCAGGCCATTATGACTGCAGCAGCTCCACTTGCACCAGATGTCCTCAAAAAATTTGAGAACAAGTTCCCTGGTGTCCAAGTCCAAGAGGCATATGGATTGACTGAACACAGCTGCATCACACTCACTCATGCCACACCGGGTGTTATGGCAAAGAAAAACTCGGTTGGGTTCATTCTTCCAAATTTGCAAGTCAAATTCATTGACCCTGACACCGGCCGATCCCTCCCCAGAAACACCCCTGGTGAACTTTGTGTCTTGAGTCAATGTGTAATGAAAGGTTACTACAATAATGAAGAGGAGACTGCCCGGATCATCGACAATAATGGATGGCTTCACACTGGTGACATTGGCTACATAGATGATGATGAAAATGTCTTTATTGTGGATCGCATCAAGGAATTAATCAAGTACAAAGGTTTCCAAGTGGCTCCTGCTGAACTTGAGGCCATCCTTCTGAGCCATCCCTCAGTTGAAGACGTTGCAGTAGTCCCATTGCCAGATGAGGAGGCAGGGGAGATTCCAGCAGCAAGTGTTGTTTTAGCAGCTGGTGCAAAAGAGAGTGAAGAAGACATTATCAAGTTTGTGGCCTCAAATGTTGCTCACTACAAGAAAGTGAGAGTGGTGCACTTTGTGGACAGCATCCCAAAATCACCCTCTGGCAAAATTATGAGAAGGTTGGTCAAAGAGGCCCTGATAGAGAAGATCAAGGCATGCAAGCTCCACACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001676 GO:0003674 GO:0003824 GO:0004467 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006082 GO:0006139 GO:0006163 GO:0006164 GO:0006629 GO:0006631 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009259 GO:0009260 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010584 GO:0010927 GO:0012505 GO:0015645 GO:0016043 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019752 GO:0022607 GO:0030198 GO:0031956 GO:0032501 GO:0032502 GO:0032787 GO:0032989 GO:0033865 GO:0033866 GO:0033875 GO:0034030 GO:0034032 GO:0034033 GO:0034641 GO:0034654 GO:0035337 GO:0035383 GO:0035384 GO:0043062 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046390 GO:0046483 GO:0046949 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0051186 GO:0051188 GO:0055086 GO:0071616 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0080110 GO:0085029 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901568 GO:1901570 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00130 ko00360 ko00940 ko01100 ko01110 map00130 map00360 map00940 map01100 map01110
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

549

Amino Acids

59.51

Weight (kDa)

6.06

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62940
fragaria_vesca FvH4_4g23640
malus_domestica MD13G1120400.v1.1
prunus_persica Prupe.1G224800_v2.0.a1
pyrus_communis pycom13g10480
rosa_chinensis RchiOBHm_Chr4g0430101
rosa_laevigata RLG00000007003
rosa_multiflora Rmu_ssc0000150.1_g000024
rosa_roxburghii Rroxscaffold_5G00371920
rosa_rugosa Rorug04G0240200
rosa_samantha Rh4AG297300 Rh4BG303700
rosa_wichuraiana Rw4G025780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 358
Acc36I ACCTGC 2 cut(s) 358, 504
Acc65I GGTACC 1 cut(s) 439
AccB1I GGYRCC 1 cut(s) 439
AccII CGCG 1 cut(s) 746
AciI CCGC 1 cut(s) 152
AclWI GGATC 4 cut(s) 734, 1131, 1235, 1311
AcoI YGGCCR 1 cut(s) 1132
AcsI RAATTY 4 cut(s) 98, 968, 1099, 1112
AcuI CTGAAG 1 cut(s) 92
AdeI CACNNNGTG 2 cut(s) 1058, 1549
AfaI GTAC 3 cut(s) 75, 441, 1328
AfiI CCNNNNNNNGG 4 cut(s) 34, 61, 238, 1130
AflII CTTAAG 1 cut(s) 217
AgsI TTSAA 5 cut(s) 473, 503, 547, 865, 1391
AjnI CCWGG 3 cut(s) 918, 985, 1156
AleI CACNNNNGTG 1 cut(s) 1257
AloI GAACNNNNNNTCC 2 cut(s) 30, 62
AluBI AGCT 8 cut(s) 380, 414, 645, 910, 941, 1029, 1463, 1636
AluI AGCT 8 cut(s) 380, 414, 645, 910, 941, 1029, 1463, 1636
Alw21I GWGCWC 3 cut(s) 18, 61, 1545
Alw26I GTCTC 4 cut(s) 186, 228, 823, 1211
Alw44I GTGCAC 2 cut(s) 14, 1541
AlwI GGATC 4 cut(s) 734, 1131, 1235, 1311
AoxI GGCC 8 cut(s) 213, 229, 697, 921, 1132, 1362, 1503, 1605
ApaLI GTGCAC 2 cut(s) 14, 1541
ApeKI GCWGC 7 cut(s) 308, 506, 935, 938, 1029, 1442, 1460
ApoI RAATTY 4 cut(s) 98, 968, 1099, 1112
ArsI GACNNNNNNTTYG 2 cut(s) 675, 707
AseI ATTAAT 1 cut(s) 1319
Asp700I GAANNNNTTC 1 cut(s) 980
Asp718I GGTACC 1 cut(s) 439
AspA2I CCTAGG 1 cut(s) 694
AspLEI GCGC 2 cut(s) 746, 841
AspS9I GGNCC 1 cut(s) 1606
AsuC2I CCSGG 2 cut(s) 1056, 1225
AsuHPI GGTGA 7 cut(s) 166, 199, 470, 787, 1172, 1271, 1560
AsuII TTCGAA 2 cut(s) 754, 789
AvrII CCTAGG 1 cut(s) 694
BaeGI GKGCMC 3 cut(s) 18, 568, 1545
BanI GGYRCC 1 cut(s) 439
BbsI GAAGAC 2 cut(s) 1398, 1491
Bbv12I GWGCWC 3 cut(s) 18, 61, 1545
BbvCI CCTCAGC 1 cut(s) 641
BbvI GCAGC 7 cut(s) 295, 518, 947, 950, 1016, 1454, 1472
BccI CCATC 5 cut(s) 346, 772, 1239, 1373, 1386
BciT130I CCWGG 3 cut(s) 920, 987, 1158
BcnI CCSGG 2 cut(s) 1056, 1225
BcoDI GTCTC 4 cut(s) 186, 228, 823, 1211
BfaI CTAG 3 cut(s) 198, 629, 695
BfmI CTRYAG 1 cut(s) 933
BfrI CTTAAG 1 cut(s) 217
BfuAI ACCTGC 2 cut(s) 358, 504
BisI GCNGC 7 cut(s) 309, 507, 936, 939, 1030, 1443, 1461
BlnI CCTAGG 1 cut(s) 694
BlsI GCNGC 7 cut(s) 310, 508, 937, 940, 1031, 1444, 1462
Bme1390I CCNGG 5 cut(s) 920, 987, 1056, 1158, 1225
BmgT120I GGNCC 1 cut(s) 1606
BmiI GGNNCC 2 cut(s) 441, 1347
BmrFI CCNGG 5 cut(s) 920, 987, 1056, 1158, 1225
BmsI GCATC 6 cut(s) 103, 359, 373, 1041, 1317, 1566
BpiI GAAGAC 2 cut(s) 1398, 1491
BpmI CTGGAG 1 cut(s) 902
Bpu10I CCTNAGC 1 cut(s) 641
Bpu14I TTCGAA 2 cut(s) 754, 789
BpuEI CTTGAG 2 cut(s) 1195, 1379
BpuMI CCSGG 2 cut(s) 1056, 1225
BsaI GGTCTC 2 cut(s) 228, 823
BsaJI CCNNGG 4 cut(s) 210, 694, 985, 1156
BsaWI WCCGGW 1 cut(s) 158
BsaXI ACNNNNNCTCC 2 cut(s) 629, 659
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 61, 238, 1130
Bse118I RCCGGY 2 cut(s) 61, 1130
Bse1I ACTGG 3 cut(s) 443, 736, 1261
Bse3DI GCAATG 2 cut(s) 89, 1409
BseBI CCWGG 3 cut(s) 920, 987, 1158
BseDI CCNNGG 4 cut(s) 210, 694, 985, 1156
BseGI GGATG 5 cut(s) 118, 1250, 1365, 1378, 1557
BseLI CCNNNNNNNGG 4 cut(s) 34, 61, 238, 1130
BseMI GCAATG 2 cut(s) 89, 1409
BseMII CTCAG 5 cut(s) 36, 655, 893, 1364, 1398
BseNI ACTGG 3 cut(s) 443, 736, 1261
BseRI GAGGAG 2 cut(s) 1229, 1436
BseSI GKGCMC 3 cut(s) 18, 568, 1545
BseX3I CGGCCG 1 cut(s) 1132
BseXI GCAGC 7 cut(s) 295, 518, 947, 950, 1016, 1454, 1472
Bsh1236I CGCG 1 cut(s) 746
Bsh1285I CGRYCG 1 cut(s) 1135
BshFI GGCC 8 cut(s) 215, 231, 699, 923, 1134, 1364, 1505, 1607
BshNI GGYRCC 1 cut(s) 439
BsiEI CGRYCG 1 cut(s) 1135
BsiHKAI GWGCWC 3 cut(s) 18, 61, 1545
BsiSI CCGG 7 cut(s) 62, 159, 519, 668, 1055, 1131, 1225
BslFI GGGAC 1 cut(s) 1391
BslI CCNNNNNNNGG 4 cut(s) 34, 61, 238, 1130
BsmAI GTCTC 4 cut(s) 186, 228, 823, 1211
BsmFI GGGAC 1 cut(s) 1391
BsmI GAATGC 1 cut(s) 140
BsnI GGCC 8 cut(s) 215, 231, 699, 923, 1134, 1364, 1505, 1607
Bso31I GGTCTC 2 cut(s) 228, 823
Bsp119I TTCGAA 2 cut(s) 754, 789
Bsp1286I GDGCHC 4 cut(s) 18, 61, 568, 1545
Bsp143I GATC 8 cut(s) 390, 556, 726, 898, 1136, 1227, 1303, 1620
BspACI CCGC 1 cut(s) 152
BspANI GGCC 8 cut(s) 215, 231, 699, 923, 1134, 1364, 1505, 1607
BspCNI CTCAG 5 cut(s) 35, 654, 894, 1365, 1397
BspFNI CGCG 1 cut(s) 746
BspLI GGNNCC 2 cut(s) 441, 1347
BspMAI CTGCAG 1 cut(s) 937
BspMI ACCTGC 2 cut(s) 358, 504
BspPI GGATC 4 cut(s) 734, 1131, 1235, 1311
BspT104I TTCGAA 2 cut(s) 754, 789
BspT107I GGYRCC 1 cut(s) 439
BspTI CTTAAG 1 cut(s) 217
BspTNI GGTCTC 2 cut(s) 228, 823
BsrDI GCAATG 2 cut(s) 89, 1409
BsrFI RCCGGY 2 cut(s) 61, 1130
BsrI ACTGG 3 cut(s) 443, 736, 1261
BssAI RCCGGY 2 cut(s) 61, 1130
BssECI CCNNGG 4 cut(s) 210, 694, 985, 1156
BssMI GATC 8 cut(s) 390, 556, 726, 898, 1136, 1227, 1303, 1620
BssT1I CCWWGG 2 cut(s) 210, 694
Bst2UI CCWGG 3 cut(s) 920, 987, 1158
Bst4CI ACNGT 3 cut(s) 515, 597, 691
Bst6I CTCTTC 1 cut(s) 1206
BstAFI CTTAAG 1 cut(s) 217
BstAPI GCANNNNNTGC 3 cut(s) 281, 515, 1466
BstBI TTCGAA 2 cut(s) 754, 789
BstC8I GCNNGC 5 cut(s) 348, 412, 908, 1630, 1634
BstDEI CTNAG 6 cut(s) 22, 233, 641, 902, 1373, 1384
BstEII GGTNACC 1 cut(s) 154
BstENI CCTNNNNNAGG 1 cut(s) 236
BstF5I GGATG 5 cut(s) 118, 1250, 1365, 1378, 1557
BstFNI CGCG 1 cut(s) 746
BstHHI GCGC 2 cut(s) 746, 841
BstKTI GATC 8 cut(s) 393, 559, 729, 901, 1139, 1230, 1306, 1623
BstMAI GTCTC 4 cut(s) 186, 228, 823, 1211
BstMBI GATC 8 cut(s) 390, 556, 726, 898, 1136, 1227, 1303, 1620
BstMCI CGRYCG 1 cut(s) 1135
BstMWI GCNNNNNNNGC 6 cut(s) 281, 290, 515, 847, 947, 1466
BstNI CCWGG 3 cut(s) 920, 987, 1158
BstNSI RCATGY 3 cut(s) 248, 350, 1632
BstPI GGTNACC 1 cut(s) 154
BstSCI CCNGG 5 cut(s) 918, 985, 1054, 1156, 1223
BstSFI CTRYAG 1 cut(s) 933
BstSLI GKGCMC 3 cut(s) 18, 568, 1545
BstUI CGCG 1 cut(s) 746
BstV1I GCAGC 7 cut(s) 295, 518, 947, 950, 1016, 1454, 1472
BstV2I GAAGAC 2 cut(s) 1398, 1491
BstZI CGGCCG 1 cut(s) 1132
BsuRI GGCC 8 cut(s) 215, 231, 699, 923, 1134, 1364, 1505, 1607
BtsCI GGATG 5 cut(s) 118, 1250, 1365, 1378, 1557
BtsIMutI CAGTG 3 cut(s) 687, 729, 1254
BveI ACCTGC 2 cut(s) 358, 504
Cac8I GCNNGC 5 cut(s) 348, 412, 908, 1630, 1634
CfoI GCGC 2 cut(s) 746, 841
Cfr10I RCCGGY 2 cut(s) 61, 1130
Cfr13I GGNCC 1 cut(s) 1606
CseI GACGC 1 cut(s) 757
Csp6I GTAC 3 cut(s) 74, 440, 1327
CspCI CAANNNNNGTGG 2 cut(s) 637, 672
CviAII CATG 4 cut(s) 245, 347, 1046, 1629
CviQI GTAC 3 cut(s) 74, 440, 1327
DdeI CTNAG 6 cut(s) 22, 233, 641, 902, 1373, 1384
DpnI GATC 8 cut(s) 392, 558, 728, 900, 1138, 1229, 1305, 1622
DpnII GATC 8 cut(s) 390, 556, 726, 898, 1136, 1227, 1303, 1620
DraIII CACNNNGTG 2 cut(s) 1058, 1549
EaeI YGGCCR 1 cut(s) 1132
EagI CGGCCG 1 cut(s) 1132
Eam1104I CTCTTC 1 cut(s) 1206
EarI CTCTTC 1 cut(s) 1206
EclXI CGGCCG 1 cut(s) 1132
Eco130I CCWWGG 2 cut(s) 210, 694
Eco147I AGGCCT 2 cut(s) 215, 699
Eco31I GGTCTC 2 cut(s) 228, 823
Eco52I CGGCCG 1 cut(s) 1132
Eco57I CTGAAG 1 cut(s) 92
Eco91I GGTNACC 1 cut(s) 154
EcoNI CCTNNNNNAGG 1 cut(s) 236
EcoO109I RGGNCCY 1 cut(s) 1606
EcoO65I GGTNACC 1 cut(s) 154
EcoRII CCWGG 3 cut(s) 918, 985, 1156
EcoT14I CCWWGG 2 cut(s) 210, 694
EcoT22I ATGCAT 2 cut(s) 352, 814
ErhI CCWWGG 2 cut(s) 210, 694
FaeI CATG 4 cut(s) 248, 350, 1049, 1632
FaqI GGGAC 1 cut(s) 1391
FatI CATG 4 cut(s) 244, 346, 1045, 1628
FauNDI CATATG 2 cut(s) 835, 1009
Fnu4HI GCNGC 7 cut(s) 309, 507, 936, 939, 1030, 1443, 1461
FokI GGATG 5 cut(s) 125, 1257, 1352, 1365, 1544
Fsp4HI GCNGC 7 cut(s) 309, 507, 936, 939, 1030, 1443, 1461
FspBI CTAG 3 cut(s) 198, 629, 695
GlaI GCGC 2 cut(s) 745, 840
GluI GCNGC 7 cut(s) 309, 507, 936, 939, 1030, 1443, 1461
GsuI CTGGAG 1 cut(s) 902
HaeIII GGCC 8 cut(s) 215, 231, 699, 923, 1134, 1364, 1505, 1607
HapII CCGG 7 cut(s) 62, 159, 519, 668, 1055, 1131, 1225
HgaI GACGC 1 cut(s) 757
HhaI GCGC 2 cut(s) 746, 841
Hin1II CATG 4 cut(s) 248, 350, 1049, 1632
Hin6I GCGC 2 cut(s) 744, 839
HinP1I GCGC 2 cut(s) 744, 839
HinfI GANTC 5 cut(s) 616, 786, 877, 1177, 1435
HpaII CCGG 7 cut(s) 62, 159, 519, 668, 1055, 1131, 1225
HphI GGTGA 7 cut(s) 166, 199, 470, 787, 1172, 1271, 1560
Hpy166II GTNNAC 4 cut(s) 16, 1163, 1543, 1552
Hpy188I TCNGA 6 cut(s) 50, 355, 556, 798, 903, 1374
Hpy188III TCNNGA 3 cut(s) 110, 327, 1174
Hpy8I GTNNAC 4 cut(s) 16, 1163, 1543, 1552
Hpy99I CGWCG 1 cut(s) 751
HpyAV CCTTC 6 cut(s) 467, 578, 583, 595, 1379, 1584
HpyCH4III ACNGT 3 cut(s) 515, 597, 691
HpyCH4IV ACGT 1 cut(s) 1395
HpyF10VI GCNNNNNNNGC 6 cut(s) 281, 290, 515, 847, 947, 1466
HpyF3I CTNAG 6 cut(s) 22, 233, 641, 902, 1373, 1384
HpySE526I ACGT 1 cut(s) 1395
Hsp92II CATG 4 cut(s) 248, 350, 1049, 1632
HspAI GCGC 2 cut(s) 744, 839
KpnI GGTACC 1 cut(s) 443
Kzo9I GATC 8 cut(s) 390, 556, 726, 898, 1136, 1227, 1303, 1620
LmnI GCTCC 5 cut(s) 329, 650, 946, 1351, 1641
Lsp1109I GCAGC 7 cut(s) 295, 518, 947, 950, 1016, 1454, 1472
LweI GCATC 6 cut(s) 103, 359, 373, 1041, 1317, 1566
MaeI CTAG 3 cut(s) 198, 629, 695
MaeII ACGT 1 cut(s) 1395
MaeIII GTNAC 8 cut(s) 85, 154, 397, 661, 685, 829, 1196, 1259
MalI GATC 8 cut(s) 392, 558, 728, 900, 1138, 1229, 1305, 1622
MboI GATC 8 cut(s) 390, 556, 726, 898, 1136, 1227, 1303, 1620
MboII GAAGA 8 cut(s) 473, 795, 1085, 1223, 1403, 1493, 1496, 1630
MhlI GDGCHC 4 cut(s) 18, 61, 568, 1545
MlyI GAGTC 2 cut(s) 610, 1186
Mph1103I ATGCAT 2 cut(s) 352, 814
MroXI GAANNNNTTC 1 cut(s) 980
MseI TTAA 5 cut(s) 218, 360, 807, 873, 1319
MslI CAYNNNNRTG 2 cut(s) 720, 1257
MspA1I CMGCKG 2 cut(s) 1029, 1463
MspCI CTTAAG 1 cut(s) 217
MspI CCGG 7 cut(s) 62, 159, 519, 668, 1055, 1131, 1225
MspR9I CCNGG 5 cut(s) 920, 987, 1056, 1158, 1225
Mva1269I GAATGC 1 cut(s) 140
MvaI CCWGG 3 cut(s) 920, 987, 1158
MvnI CGCG 1 cut(s) 746
MwoI GCNNNNNNNGC 6 cut(s) 281, 290, 515, 847, 947, 1466
NciI CCSGG 2 cut(s) 1056, 1225
NdeI CATATG 2 cut(s) 835, 1009
NdeII GATC 8 cut(s) 390, 556, 726, 898, 1136, 1227, 1303, 1620
NlaIII CATG 4 cut(s) 248, 350, 1049, 1632
NlaIV GGNNCC 2 cut(s) 441, 1347
NmeAIII GCCGAG 1 cut(s) 80
NmuCI GTSAC 5 cut(s) 85, 154, 685, 829, 1259
NsiI ATGCAT 2 cut(s) 352, 814
NspI RCATGY 3 cut(s) 248, 350, 1632
NspV TTCGAA 2 cut(s) 754, 789
OliI CACNNNNGTG 1 cut(s) 1257
PaeI GCATGC 2 cut(s) 350, 1632
PaqCI CACCTGC 1 cut(s) 358
PceI AGGCCT 2 cut(s) 215, 699
PctI GAATGC 1 cut(s) 140
PdmI GAANNNNTTC 1 cut(s) 980
PfeI GAWTC 3 cut(s) 786, 877, 1435
PkrI GCNGC 7 cut(s) 310, 508, 937, 940, 1031, 1444, 1462
PleI GAGTC 2 cut(s) 610, 1185
PpsI GAGTC 2 cut(s) 610, 1185
PshBI ATTAAT 1 cut(s) 1319
Psp6I CCWGG 3 cut(s) 918, 985, 1156
PspEI GGTNACC 1 cut(s) 154
PspGI CCWGG 3 cut(s) 918, 985, 1156
PspN4I GGNNCC 2 cut(s) 441, 1347
PspPI GGNCC 1 cut(s) 1606
PstI CTGCAG 1 cut(s) 937
PvuII CAGCTG 2 cut(s) 1029, 1463
RsaI GTAC 3 cut(s) 75, 441, 1328
RsaNI GTAC 3 cut(s) 74, 440, 1327
RseI CAYNNNNRTG 2 cut(s) 720, 1257
SaqAI TTAA 5 cut(s) 218, 360, 807, 873, 1319
SatI GCNGC 7 cut(s) 309, 507, 936, 939, 1030, 1443, 1461
Sau3AI GATC 8 cut(s) 390, 556, 726, 898, 1136, 1227, 1303, 1620
Sau96I GGNCC 1 cut(s) 1606
SchI GAGTC 2 cut(s) 610, 1186
ScrFI CCNGG 5 cut(s) 920, 987, 1056, 1158, 1225
SduI GDGCHC 4 cut(s) 18, 61, 568, 1545
SfaNI GCATC 6 cut(s) 103, 359, 373, 1041, 1317, 1566
SfcI CTRYAG 1 cut(s) 933
SfuI TTCGAA 2 cut(s) 754, 789
SmiMI CAYNNNNRTG 2 cut(s) 720, 1257
SmlI CTYRAG 3 cut(s) 217, 1174, 1358
SmoI CTYRAG 3 cut(s) 217, 1174, 1358
SphI GCATGC 2 cut(s) 350, 1632
SseBI AGGCCT 2 cut(s) 215, 699
SsiI CCGC 1 cut(s) 152
SspMI CTAG 3 cut(s) 198, 629, 695
StuI AGGCCT 2 cut(s) 215, 699
StyD4I CCNGG 5 cut(s) 918, 985, 1054, 1156, 1223
StyI CCWWGG 2 cut(s) 210, 694
TaaI ACNGT 3 cut(s) 515, 597, 691
TaiI ACGT 1 cut(s) 1398
TaqI TCGA 5 cut(s) 599, 622, 754, 789, 1233
TatI WGTACW 1 cut(s) 1326
TfiI GAWTC 3 cut(s) 786, 877, 1435
Tru1I TTAA 5 cut(s) 218, 360, 807, 873, 1319
Tru9I TTAA 5 cut(s) 218, 360, 807, 873, 1319
TscAI CASTG 3 cut(s) 694, 736, 1261
TseFI GTSAC 5 cut(s) 85, 154, 685, 829, 1259
TseI GCWGC 7 cut(s) 308, 506, 935, 938, 1029, 1442, 1460
Tsp45I GTSAC 5 cut(s) 85, 154, 685, 829, 1259
TspDTI ATGAA 7 cut(s) 432, 497, 1078, 1105, 1205, 1224, 1299
TspRI CASTG 3 cut(s) 694, 736, 1261
Vha464I CTTAAG 1 cut(s) 217
VneI GTGCAC 2 cut(s) 14, 1541
VspI ATTAAT 1 cut(s) 1319
XagI CCTNNNNNAGG 1 cut(s) 236
XapI RAATTY 4 cut(s) 98, 968, 1099, 1112
XceI RCATGY 3 cut(s) 248, 350, 1632
XcmI CCANNNNNNNNNTGG 2 cut(s) 292, 1007
XmaJI CCTAGG 1 cut(s) 694
XmnI GAANNNNTTC 1 cut(s) 980
XspI CTAG 3 cut(s) 198, 629, 695
Zsp2I ATGCAT 2 cut(s) 352, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.