FvH4_4g24820

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017652
Physical Location & Seq
Forward (+)
26823381 .. 26824904
1524 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24820.t1

Sequence Viewer

Length: 1524 bp
ATGGAATCTGGTTTCTATGTGCAACCTTGTGAAGTCTATGGTGATAACAAATGTGATGAGGTTGTTGGTCTTGATCTTAATCTCTCATCCATGTCATGTCTTCCCTTCTCTTCTCTTTCGACTCTGGAGAACAGTTCTGCTGTCTGGGAGATTCCGTTCATAGATGAAACAAGAAATCTTAAGAGGGTAAAGCTTGAAAATAGTGCTGATGAATCCATTGTAAGTACTAATTATAGTCTTTATAGTGGCAGTGGCAGCATGTATACTGGTGGGTTCAATAGCTTGTCAAGAATTCAAATCCGGGATCACATATCAGCTTACACCCGGAGATACATGGCAGCAGAAGCCATGGAAGAAGCGGCTTCAACCTTTCTGGGAGGTGAACCTGAGGAAGAAGGAAGTGGAGATGGGATGAAACTAGTTCAAAAACTCATTTCGTGCGCTGAGGCCGTGGCTTGTCGTGACAAAACTCATGCTTCAGCATTGCTGTATGAGCTTAGAACTAATGCCCAGGTCTTTGGGACATCATTCCAGCGGGTAGCTTCCTGCTTTATCCAAGCTCTCTCTGACCGTCTTGCTCTTGTTCAACCACTGGGAGCTGTGGGAGTTACTACAAAGATAATTGGTGTTTCATCAGAGAAGGATGAGGCTTTGCACCTTGTTTATGACATTTGCCCACATATAAGGTTCAGTCATTTTGTAGCAAACACATCAATATTGGAAGCCTTTGAGGGAGAGAGCTCTGTTCATGTCATAGACTTGGGCATGACCCTAGGCCTCCTACATGGGTACCAATGGCGCAACTTGATAGACAGACTGGCCAGCCGAACAGGACGCTTACCTCGCCTTCGAATAACTGGTGTTGGTAACTCTTGTGAACGTCTTGAAGCGATTGGTAATGAACTAAAGCTGTATGCACAAAACGTCGAGCTGGAATTTGAATTTTTAGCACTGGAAAGTAACTTGGAAAACCTGAAGGCAGAAGATTTCAGTCTCATTGATGGGGAGGTTTTAGTCATCAATAGCATACTCCAGCTGCATTGTCTTGTGAAAGAGAGTAGAGGAGCATTGAACTCAGTTCTACAAACACTATATGAGCTATCACCAAAGCTAATGGTTCTAGTTGAGCAGGACTCGAGCCACAATGGGCCCTTCTTTCTTGGGAGGTTCATGGAGGCATTGCATAACTACTCTGCCATCTTCGACTCCTTAGATGCAATGTTGCCCAAGTATGACACAAGGAGAGCTAAAGTGGAGCAGTTTTATTTTGGAGAGGAGATTAAGAACATAGTAAGCTGCGAGGGACCTGCGAGAGTGGAGCGGCACGAAAGGATTGATCAATGGCGCAGGAGGATGAGGCGCGCCGGGTTCCAACCGGCGCCCCTGAAGACAATTACGCAGGCCACAAAATGGCTAGAAAAGAATGCTAGTGAAGGTTACACAATTGTGGAAGACAAGGGCTGCTTTGTTCTTGGGTGGAAATCAAAGCCTATCATTGCAGCTTCATGCTGGAGGTGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

508

Amino Acids

56.66

Weight (kDa)

5.41

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 140 - 505 2.8e-110 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017652)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24820
malus_domestica MD13G1108700.v1.1
prunus_persica Prupe.1G237500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0431901
rosa_laevigata RLG00000006877
rosa_roxburghii Rroxscaffold_5G00373330
rosa_rugosa Rorug04G0252900
rosa_samantha Rh4AG310100 Rh4BG317200 Rh4CG332500 Rh4DG312100
rosa_wichuraiana Rw4G026980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1315
Acc65I GGTACC 1 cut(s) 789
AccB1I GGYRCC 2 cut(s) 789, 1378
AccB7I CCANNNNNTGG 1 cut(s) 1410
AccBSI CCGCTC 1 cut(s) 1321
AccI GTMKAC 1 cut(s) 263
AccII CGCG 1 cut(s) 1362
AciI CCGC 3 cut(s) 359, 535, 1321
AclWI GGATC 1 cut(s) 312
AcoI YGGCCR 1 cut(s) 819
AcsI RAATTY 3 cut(s) 291, 935, 941
AcuI CTGAAG 3 cut(s) 462, 995, 1406
AcyI GRCGYC 1 cut(s) 1379
AfaI GTAC 2 cut(s) 226, 791
AfiI CCNNNNNNNGG 1 cut(s) 1410
AflII CTTAAG 1 cut(s) 179
AgsI TTSAA 9 cut(s) 197, 277, 296, 366, 425, 587, 887, 941, 1072
AhlI ACTAGT 1 cut(s) 418
AjnI CCWGG 1 cut(s) 510
AleI CACNNNNGTG 1 cut(s) 1445
AloI GAACNNNNNNTCC 2 cut(s) 140, 172
Alw21I GWGCWC 1 cut(s) 743
Alw26I GTCTC 1 cut(s) 998
AlwI GGATC 1 cut(s) 312
Ama87I CYCGRG 1 cut(s) 1135
AoxI GGCC 5 cut(s) 447, 775, 819, 1148, 1401
ApaI GGGCCC 1 cut(s) 1152
ApeKI GCWGC 6 cut(s) 255, 338, 1036, 1296, 1461, 1499
ApoI RAATTY 3 cut(s) 291, 935, 941
AscI GGCGCGCC 1 cut(s) 1360
Asp718I GGTACC 1 cut(s) 789
AspA2I CCTAGG 1 cut(s) 772
AspLEI GCGC 6 cut(s) 443, 801, 1347, 1362, 1364, 1381
AspS9I GGNCC 3 cut(s) 1148, 1149, 1304
AsuC2I CCSGG 3 cut(s) 302, 325, 1366
AsuHPI GGTGA 3 cut(s) 53, 392, 1095
AsuII TTCGAA 1 cut(s) 850
AvaI CYCGRG 1 cut(s) 1135
AvaII GGWCC 1 cut(s) 1304
AvrII CCTAGG 1 cut(s) 772
AxyI CCTNAGG 1 cut(s) 387
BaeGI GKGCMC 1 cut(s) 1152
BaeI ACNNNNGTAYC 2 cut(s) 773, 806
BalI TGGCCA 1 cut(s) 821
BanI GGYRCC 2 cut(s) 789, 1378
BanII GRGCYC 2 cut(s) 743, 1152
BbsI GAAGAC 3 cut(s) 92, 1394, 1458
Bbv12I GWGCWC 1 cut(s) 743
BbvCI CCTCAGC 1 cut(s) 444
BbvI GCAGC 6 cut(s) 267, 350, 1023, 1283, 1448, 1511
BccI CCATC 3 cut(s) 401, 995, 1205
BceAI ACGGC 1 cut(s) 434
BcgI CGANNNNNNTGC 2 cut(s) 1289, 1323
BciT130I CCWGG 1 cut(s) 512
BclI TGATCA 1 cut(s) 1336
BcnI CCSGG 3 cut(s) 302, 325, 1366
BcoDI GTCTC 1 cut(s) 998
BcuI ACTAGT 1 cut(s) 418
BfaI CTAG 5 cut(s) 419, 773, 1121, 1415, 1428
BfoI RGCGCY 1 cut(s) 1382
BfrI CTTAAG 1 cut(s) 179
BfuAI ACCTGC 1 cut(s) 1315
BisI GCNGC 8 cut(s) 256, 339, 360, 1037, 1297, 1322, 1462, 1500
BlnI CCTAGG 1 cut(s) 772
BlsI GCNGC 8 cut(s) 257, 340, 361, 1038, 1298, 1323, 1463, 1501
BmcAI AGTACT 1 cut(s) 226
Bme1390I CCNGG 4 cut(s) 302, 325, 512, 1366
Bme18I GGWCC 1 cut(s) 1304
BmeT110I CYCGRG 1 cut(s) 1135
BmgT120I GGNCC 3 cut(s) 1148, 1149, 1304
BmiI GGNNCC 5 cut(s) 791, 1150, 1305, 1370, 1380
BmrFI CCNGG 4 cut(s) 302, 325, 512, 1366
BmrI ACTGGG 1 cut(s) 602
BmsI GCATC 1 cut(s) 1204
BmuI ACTGGG 1 cut(s) 602
BpiI GAAGAC 3 cut(s) 92, 1394, 1458
BplI GAGNNNNNCTC 2 cut(s) 1118, 1150
BpmI CTGGAG 2 cut(s) 146, 1016
Bpu10I CCTNAGC 1 cut(s) 444
Bpu14I TTCGAA 1 cut(s) 850
BpuMI CCSGG 3 cut(s) 302, 325, 1366
BsaBI GATNNNNATC 1 cut(s) 78
BsaHI GRCGYC 1 cut(s) 1379
BsaJI CCNNGG 4 cut(s) 348, 450, 510, 772
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 1 cut(s) 1410
Bse118I RCCGGY 1 cut(s) 1375
Bse1I ACTGG 5 cut(s) 271, 597, 822, 862, 957
Bse21I CCTNAGG 1 cut(s) 387
Bse3DI GCAATG 4 cut(s) 482, 1178, 1224, 1494
Bse8I GATNNNNATC 1 cut(s) 78
BseBI CCWGG 1 cut(s) 512
BseDI CCNNGG 4 cut(s) 348, 450, 510, 772
BseGI GGATG 4 cut(s) 86, 417, 649, 1359
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 1 cut(s) 1410
BseMI GCAATG 4 cut(s) 482, 1178, 1224, 1494
BseMII CTCAG 3 cut(s) 378, 435, 1089
BseNI ACTGG 5 cut(s) 271, 597, 822, 862, 957
BsePI GCGCGC 1 cut(s) 1360
BseRI GAGGAG 2 cut(s) 1077, 1289
BseSI GKGCMC 1 cut(s) 1152
BseXI GCAGC 6 cut(s) 267, 350, 1023, 1283, 1448, 1511
Bsh1236I CGCG 1 cut(s) 1362
BshFI GGCC 5 cut(s) 449, 777, 821, 1150, 1403
BshNI GGYRCC 2 cut(s) 789, 1378
BsiHKAI GWGCWC 1 cut(s) 743
BsiHKCI CYCGRG 1 cut(s) 1135
BsiSI CCGG 4 cut(s) 301, 325, 1365, 1376
BslFI GGGAC 2 cut(s) 535, 1317
BslI CCNNNNNNNGG 1 cut(s) 1410
BsmAI GTCTC 1 cut(s) 998
BsmFI GGGAC 2 cut(s) 535, 1317
BsmI GAATGC 1 cut(s) 1429
BsnI GGCC 5 cut(s) 449, 777, 821, 1150, 1403
BsoBI CYCGRG 1 cut(s) 1135
Bsp119I TTCGAA 1 cut(s) 850
Bsp120I GGGCCC 1 cut(s) 1148
Bsp1286I GDGCHC 2 cut(s) 743, 1152
Bsp143I GATC 3 cut(s) 73, 304, 1336
Bsp19I CCATGG 1 cut(s) 348
BspACI CCGC 3 cut(s) 359, 535, 1321
BspANI GGCC 5 cut(s) 449, 777, 821, 1150, 1403
BspCNI CTCAG 3 cut(s) 379, 436, 1088
BspFNI CGCG 1 cut(s) 1362
BspLI GGNNCC 5 cut(s) 791, 1150, 1305, 1370, 1380
BspMI ACCTGC 1 cut(s) 1315
BspPI GGATC 1 cut(s) 312
BspT104I TTCGAA 1 cut(s) 850
BspT107I GGYRCC 2 cut(s) 789, 1378
BspTI CTTAAG 1 cut(s) 179
BsrBI CCGCTC 1 cut(s) 1321
BsrDI GCAATG 4 cut(s) 482, 1178, 1224, 1494
BsrFI RCCGGY 1 cut(s) 1375
BsrI ACTGG 5 cut(s) 271, 597, 822, 862, 957
BssAI RCCGGY 1 cut(s) 1375
BssECI CCNNGG 4 cut(s) 348, 450, 510, 772
BssHII GCGCGC 1 cut(s) 1360
BssMI GATC 3 cut(s) 73, 304, 1336
BssNAI GTATAC 1 cut(s) 264
BssNI GRCGYC 1 cut(s) 1379
BssT1I CCWWGG 2 cut(s) 348, 772
Bst1107I GTATAC 1 cut(s) 264
Bst2UI CCWGG 1 cut(s) 512
Bst4CI ACNGT 2 cut(s) 134, 572
Bst6I CTCTTC 1 cut(s) 115
BstACI GRCGYC 1 cut(s) 1379
BstAFI CTTAAG 1 cut(s) 179
BstBI TTCGAA 1 cut(s) 850
BstC8I GCNNGC 3 cut(s) 823, 1362, 1401
BstDEI CTNAG 5 cut(s) 387, 444, 497, 1075, 1210
BstDSI CCRYGG 2 cut(s) 348, 450
BstF5I GGATG 4 cut(s) 86, 417, 649, 1359
BstFNI CGCG 1 cut(s) 1362
BstH2I RGCGCY 1 cut(s) 1382
BstHHI GCGC 6 cut(s) 443, 801, 1347, 1362, 1364, 1381
BstKTI GATC 3 cut(s) 76, 307, 1339
BstMAI GTCTC 1 cut(s) 998
BstMBI GATC 3 cut(s) 73, 304, 1336
BstMWI GCNNNNNNNGC 4 cut(s) 255, 344, 493, 843
BstNI CCWGG 1 cut(s) 512
BstNSI RCATGY 1 cut(s) 262
BstSCI CCNGG 4 cut(s) 300, 323, 510, 1364
BstSLI GKGCMC 1 cut(s) 1152
BstUI CGCG 1 cut(s) 1362
BstV1I GCAGC 6 cut(s) 267, 350, 1023, 1283, 1448, 1511
BstV2I GAAGAC 3 cut(s) 92, 1394, 1458
BstXI CCANNNNNNTGG 1 cut(s) 518
BstZ17I GTATAC 1 cut(s) 264
Bsu36I CCTNAGG 1 cut(s) 387
BsuRI GGCC 5 cut(s) 449, 777, 821, 1150, 1403
BtgI CCRYGG 2 cut(s) 348, 450
BtsCI GGATG 4 cut(s) 86, 417, 649, 1359
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 3 cut(s) 256, 590, 950
BveI ACCTGC 1 cut(s) 1315
Cac8I GCNNGC 3 cut(s) 823, 1362, 1401
CfoI GCGC 6 cut(s) 443, 801, 1347, 1362, 1364, 1381
Cfr10I RCCGGY 1 cut(s) 1375
Cfr13I GGNCC 3 cut(s) 1148, 1149, 1304
CseI GACGC 1 cut(s) 843
Csp6I GTAC 2 cut(s) 225, 790
CviQI GTAC 2 cut(s) 225, 790
DdeI CTNAG 5 cut(s) 387, 444, 497, 1075, 1210
DinI GGCGCC 1 cut(s) 1380
DpnI GATC 3 cut(s) 75, 306, 1338
DpnII GATC 3 cut(s) 73, 304, 1336
EaeI YGGCCR 1 cut(s) 819
Eam1104I CTCTTC 1 cut(s) 115
EarI CTCTTC 1 cut(s) 115
Ecl136II GAGCTC 1 cut(s) 741
Eco130I CCWWGG 2 cut(s) 348, 772
Eco147I AGGCCT 1 cut(s) 777
Eco24I GRGCYC 2 cut(s) 743, 1152
Eco47I GGWCC 1 cut(s) 1304
Eco53kI GAGCTC 1 cut(s) 741
Eco57I CTGAAG 3 cut(s) 462, 995, 1406
Eco81I CCTNAGG 1 cut(s) 387
Eco88I CYCGRG 1 cut(s) 1135
EcoICRI GAGCTC 1 cut(s) 741
EcoO109I RGGNCCY 2 cut(s) 1149, 1304
EcoRI GAATTC 1 cut(s) 291
EcoRII CCWGG 1 cut(s) 510
EcoT14I CCWWGG 2 cut(s) 348, 772
EcoT38I GRGCYC 2 cut(s) 743, 1152
EgeI GGCGCC 1 cut(s) 1380
EheI GGCGCC 1 cut(s) 1380
ErhI CCWWGG 2 cut(s) 348, 772
FalI AAGNNNNNCTT 2 cut(s) 1448, 1480
FaqI GGGAC 2 cut(s) 535, 1317
FauI CCCGC 1 cut(s) 528
FbaI TGATCA 1 cut(s) 1336
FblI GTMKAC 1 cut(s) 263
Fnu4HI GCNGC 8 cut(s) 256, 339, 360, 1037, 1297, 1322, 1462, 1500
FokI GGATG 4 cut(s) 73, 424, 656, 1366
FriOI GRGCYC 2 cut(s) 743, 1152
Fsp4HI GCNGC 8 cut(s) 256, 339, 360, 1037, 1297, 1322, 1462, 1500
FspBI CTAG 5 cut(s) 419, 773, 1121, 1415, 1428
GlaI GCGC 6 cut(s) 442, 800, 1346, 1361, 1363, 1380
GluI GCNGC 8 cut(s) 256, 339, 360, 1037, 1297, 1322, 1462, 1500
GsuI CTGGAG 2 cut(s) 146, 1016
HaeII RGCGCY 1 cut(s) 1382
HaeIII GGCC 5 cut(s) 449, 777, 821, 1150, 1403
HapII CCGG 4 cut(s) 301, 325, 1365, 1376
HgaI GACGC 1 cut(s) 843
HhaI GCGC 6 cut(s) 443, 801, 1347, 1362, 1364, 1381
Hin1I GRCGYC 1 cut(s) 1379
Hin6I GCGC 6 cut(s) 441, 799, 1345, 1360, 1362, 1379
HinP1I GCGC 6 cut(s) 441, 799, 1345, 1360, 1362, 1379
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 6 cut(s) 5, 121, 151, 212, 1133, 1205
HpaII CCGG 4 cut(s) 301, 325, 1365, 1376
HphI GGTGA 3 cut(s) 53, 392, 1095
Hpy166II GTNNAC 3 cut(s) 264, 383, 878
Hpy188I TCNGA 2 cut(s) 568, 637
Hpy188III TCNNGA 5 cut(s) 71, 125, 288, 461, 884
Hpy8I GTNNAC 3 cut(s) 264, 383, 878
Hpy99I CGWCG 1 cut(s) 929
HpyAV CCTTC 7 cut(s) 115, 389, 634, 857, 970, 1162, 1427
HpyCH4III ACNGT 2 cut(s) 134, 572
HpyCH4IV ACGT 2 cut(s) 880, 924
HpyCH4V TGCA 7 cut(s) 22, 655, 917, 1039, 1183, 1217, 1499
HpyF10VI GCNNNNNNNGC 4 cut(s) 255, 344, 493, 843
HpyF3I CTNAG 5 cut(s) 387, 444, 497, 1075, 1210
HpySE526I ACGT 2 cut(s) 880, 924
Hsp92I GRCGYC 1 cut(s) 1379
HspAI GCGC 6 cut(s) 441, 799, 1345, 1360, 1362, 1379
KasI GGCGCC 1 cut(s) 1378
KpnI GGTACC 1 cut(s) 793
Ksp22I TGATCA 1 cut(s) 1336
Kzo9I GATC 3 cut(s) 73, 304, 1336
LmnI GCTCC 4 cut(s) 596, 1064, 1255, 1318
Lsp1109I GCAGC 6 cut(s) 267, 350, 1023, 1283, 1448, 1511
LweI GCATC 1 cut(s) 1204
MaeI CTAG 5 cut(s) 419, 773, 1121, 1415, 1428
MaeII ACGT 2 cut(s) 880, 924
MaeIII GTNAC 5 cut(s) 461, 607, 866, 959, 1436
MalI GATC 3 cut(s) 75, 306, 1338
MbiI CCGCTC 1 cut(s) 1321
MboI GATC 3 cut(s) 73, 304, 1336
MboII GAAGA 8 cut(s) 92, 102, 365, 404, 995, 1192, 1399, 1463
MfeI CAATTG 1 cut(s) 1443
MhlI GDGCHC 2 cut(s) 743, 1152
MlsI TGGCCA 1 cut(s) 821
MluCI AATT 7 cut(s) 229, 291, 621, 935, 941, 1392, 1443
MluNI TGGCCA 1 cut(s) 821
Mly113I GGCGCC 1 cut(s) 1379
MlyI GAGTC 3 cut(s) 115, 1127, 1199
MmeI TCCRAC 1 cut(s) 1396
Mox20I TGGCCA 1 cut(s) 821
MscI TGGCCA 1 cut(s) 821
MseI TTAA 4 cut(s) 78, 180, 1281, 1522
MslI CAYNNNNRTG 1 cut(s) 1445
Msp20I TGGCCA 1 cut(s) 821
MspA1I CMGCKG 2 cut(s) 535, 1036
MspCI CTTAAG 1 cut(s) 179
MspI CCGG 4 cut(s) 301, 325, 1365, 1376
MspR9I CCNGG 4 cut(s) 302, 325, 512, 1366
MunI CAATTG 1 cut(s) 1443
Mva1269I GAATGC 1 cut(s) 1429
MvaI CCWGG 1 cut(s) 512
MvnI CGCG 1 cut(s) 1362
MwoI GCNNNNNNNGC 4 cut(s) 255, 344, 493, 843
NarI GGCGCC 1 cut(s) 1379
NciI CCSGG 3 cut(s) 302, 325, 1366
NcoI CCATGG 1 cut(s) 348
NdeII GATC 3 cut(s) 73, 304, 1336
NlaIV GGNNCC 5 cut(s) 791, 1150, 1305, 1370, 1380
NmuCI GTSAC 1 cut(s) 461
NspI RCATGY 1 cut(s) 262
NspV TTCGAA 1 cut(s) 850
OliI CACNNNNGTG 1 cut(s) 1445
PaeR7I CTCGAG 1 cut(s) 1135
PalAI GGCGCGCC 1 cut(s) 1360
PauI GCGCGC 1 cut(s) 1360
PceI AGGCCT 1 cut(s) 777
PctI GAATGC 1 cut(s) 1429
PfeI GAWTC 3 cut(s) 5, 151, 212
PflMI CCANNNNNTGG 1 cut(s) 1410
PfoI TCCNGGA 1 cut(s) 300
PkrI GCNGC 8 cut(s) 257, 340, 361, 1038, 1298, 1323, 1463, 1501
PleI GAGTC 3 cut(s) 115, 1127, 1199
PluTI GGCGCC 1 cut(s) 1382
PpsI GAGTC 3 cut(s) 115, 1127, 1199
PpuMI RGGWCCY 1 cut(s) 1304
Psp124BI GAGCTC 1 cut(s) 743
Psp5II RGGWCCY 1 cut(s) 1304
Psp6I CCWGG 1 cut(s) 510
PspGI CCWGG 1 cut(s) 510
PspN4I GGNNCC 5 cut(s) 791, 1150, 1305, 1370, 1380
PspOMI GGGCCC 1 cut(s) 1148
PspPI GGNCC 3 cut(s) 1148, 1149, 1304
PspPPI RGGWCCY 1 cut(s) 1304
PspXI VCTCGAGB 1 cut(s) 1135
PteI GCGCGC 1 cut(s) 1360
PvuII CAGCTG 1 cut(s) 1036
RsaI GTAC 2 cut(s) 226, 791
RsaNI GTAC 2 cut(s) 225, 790
RseI CAYNNNNRTG 1 cut(s) 1445
SacI GAGCTC 1 cut(s) 743
SaqAI TTAA 4 cut(s) 78, 180, 1281, 1522
SatI GCNGC 8 cut(s) 256, 339, 360, 1037, 1297, 1322, 1462, 1500
Sau3AI GATC 3 cut(s) 73, 304, 1336
Sau96I GGNCC 3 cut(s) 1148, 1149, 1304
ScaI AGTACT 1 cut(s) 226
SchI GAGTC 3 cut(s) 115, 1127, 1199
ScrFI CCNGG 4 cut(s) 302, 325, 512, 1366
SduI GDGCHC 2 cut(s) 743, 1152
SfaNI GCATC 1 cut(s) 1204
SfoI GGCGCC 1 cut(s) 1380
Sfr274I CTCGAG 1 cut(s) 1135
SfuI TTCGAA 1 cut(s) 850
SgsI GGCGCGCC 1 cut(s) 1360
SinI GGWCC 1 cut(s) 1304
SlaI CTCGAG 1 cut(s) 1135
SmiMI CAYNNNNRTG 1 cut(s) 1445
SmlI CTYRAG 2 cut(s) 179, 1135
SmoI CTYRAG 2 cut(s) 179, 1135
SpeI ACTAGT 1 cut(s) 418
Sse9I AATT 7 cut(s) 229, 291, 621, 935, 941, 1392, 1443
SseBI AGGCCT 1 cut(s) 777
SsiI CCGC 3 cut(s) 359, 535, 1321
SspDI GGCGCC 1 cut(s) 1378
SspI AATATT 1 cut(s) 717
SspMI CTAG 5 cut(s) 419, 773, 1121, 1415, 1428
SstI GAGCTC 1 cut(s) 743
StuI AGGCCT 1 cut(s) 777
StyD4I CCNGG 4 cut(s) 300, 323, 510, 1364
StyI CCWWGG 2 cut(s) 348, 772
TaaI ACNGT 2 cut(s) 134, 572
TaiI ACGT 2 cut(s) 883, 927
TaqI TCGA 5 cut(s) 119, 850, 927, 1136, 1203
TasI AATT 7 cut(s) 229, 291, 621, 935, 941, 1392, 1443
TatI WGTACW 1 cut(s) 224
TauI GCSGC 2 cut(s) 362, 1324
TfiI GAWTC 3 cut(s) 5, 151, 212
Tru1I TTAA 4 cut(s) 78, 180, 1281, 1522
Tru9I TTAA 4 cut(s) 78, 180, 1281, 1522
TscAI CASTG 3 cut(s) 256, 597, 957
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 6 cut(s) 255, 338, 1036, 1296, 1461, 1499
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 9 cut(s) 148, 180, 225, 428, 621, 737, 915, 1159, 1494
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 3 cut(s) 256, 597, 957
Van91I CCANNNNNTGG 1 cut(s) 1410
Vha464I CTTAAG 1 cut(s) 179
VpaK11BI GGWCC 1 cut(s) 1304
XapI RAATTY 3 cut(s) 291, 935, 941
XceI RCATGY 1 cut(s) 262
XhoI CTCGAG 1 cut(s) 1135
XmaJI CCTAGG 1 cut(s) 772
XmiI GTMKAC 1 cut(s) 263
XspI CTAG 5 cut(s) 419, 773, 1121, 1415, 1428
ZrmI AGTACT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.