RchiOBHm_Chr4g0431901

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0017652
Physical Location & Seq
Forward (+)
56037104 .. 56038636
1533 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40054

Sequence Viewer

Length: 1533 bp
ATGACATCTGGTTTCTATGTGCAACCTTGTGAAGTCTATGGCAATAACAAATGTGATGAGGTTATTGGTCTTGATCTCAATCTCTCATCCATGTCATGCCTTCCCTTCCCTTCTCTTTCGACTCTGGAGAACAATTCCGCTGTCTGGGAGATTCCGTTCATAGATGAAACAAGAAATCATAAGAGGGTAAAGCTTGAAGATAGTGCCAATGAATCCATTATAAGTACTAATTATAGTCTTTACAGTGGCAGTGGCACTGGCAGCATGTATACTAGTGGCTTCAATAGCTTGTCAAGAATTCAATTCCGGGATCACATATCAGCTTACACCCGGAGATACTTGGCAGCAGAGAAAATGGAAGAAGAAGCTTCAACCTTGCTGGGAGGCGAGCCCGAGGAAGAAGGAAGTGGAGATGGAATGAAACTAGTTCAAAAACTCATTTCATGCGCTGAGGCTGTGGCTTGTCGTGACAAAACTCATGCTTCAGCATTACTTTCTGAGCTTAGAACTAATGCCCAAGTCTTTGGGACATCATTCCAGCGAGTAGCTTCCTGCTTTATCCAAGCTCTCACTGACCGTCTTGCTCTCGTTCAACCACTGGGGGCTGTGGGAGTTACAACAAAGATGATTGCTGTTTCCTCAGAGAAGGATGAGGCTTTGCACCTTGTTTATGAGATTTGTCCACATATCCAGTTTAGTCATTTTGTAGCAAACACATCAATATTGGAAGCCTTTGAGGGGGAGAGCTATGTTCATGTCATAGACTTGGGCATGACCCTAGGCCTCCTTCATGGCTACCAATGGCTCAACTTGATGGAAAGTCTAGCCAGCCGAACGGGACAACCCTTACGCCGGCTTCGAATAACTGGTGTTGGTGACTCTGGTGAACGCCTGGAAGCGATTGGTAATGAACTAAAGTTGTATGCACAAAGCATGGAGCTGGATTTTGGTTTTTTAGCTGTGGAAAGCAACTTGGAAAACCTGAAGGCCGAAGATTTCAATCTCATTGATGGGGAGGTTTTAATCATCAATAGCATACTCCAGCTGCATTGTCTTGTGAAAGAGAGTAGAGGAGCATTGAACTCGGTTCTACAAACACTATATGACCTATCACCAAAGCTAATGGTTCTAGTTGAGCAGGACTCGAGCCACAATGGGCCTTTCTTTCTTGGGAGGTTCATGGAGGCATTACATAATTACTCTGCCATCTTCGACTCCTTAGATGCAATGCTGCCCAGATATGACACAAGGAGAGCTAAAATGGAGCAGTTTTATTTCGGAGAGGAGATTAAGAACATAGTAAGCTGCGAGGGACCTGCGCGAGTGGAGCGGCACGAAAGAGTTGATCAATGGCGCAGGAGGATGAGGCGCGCTGGGTTCCAACCGGCACCCCTGAAGATGATTACACAGGCTACAAAATGGCTAGAAATGAATGCTAGTGAGGGTTACACAATTGTGGAAGACAAGGGTTGCTTTGTTCTTGGGTGGAAGTCGAAGCCTATCATTGCAGTTTCTTGCTGGAGATGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

510

Amino Acids

57.14

Weight (kDa)

5.33

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 142 - 508 1e-110 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017652)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24820
malus_domestica MD13G1108700.v1.1
prunus_persica Prupe.1G237500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0431901
rosa_laevigata RLG00000006877
rosa_roxburghii Rroxscaffold_5G00373330
rosa_rugosa Rorug04G0252900
rosa_samantha Rh4AG310100 Rh4BG317200 Rh4CG332500 Rh4DG312100
rosa_wichuraiana Rw4G026980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 221
Acc36I ACCTGC 1 cut(s) 1324
AccB1I GGYRCC 1 cut(s) 1387
AccBSI CCGCTC 1 cut(s) 1330
AccI GTMKAC 1 cut(s) 269
AccII CGCG 2 cut(s) 1321, 1371
AciI CCGC 2 cut(s) 138, 1330
AclWI GGATC 1 cut(s) 318
AcsI RAATTY 1 cut(s) 297
AcuI CTGAAG 3 cut(s) 468, 1004, 1415
AfaI GTAC 1 cut(s) 226
AfiI CCNNNNNNNGG 3 cut(s) 144, 738, 852
AgsI TTSAA 8 cut(s) 197, 283, 302, 372, 431, 593, 1000, 1081
AhlI ACTAGT 2 cut(s) 272, 424
AjnI CCWGG 1 cut(s) 891
AleI CACNNNNGTG 1 cut(s) 1454
AloI GAACNNNNNNTCC 2 cut(s) 140, 172
AlwI GGATC 1 cut(s) 318
Ama87I CYCGRG 2 cut(s) 392, 1144
AoxI GGCC 3 cut(s) 781, 987, 1157
ApeKI GCWGC 5 cut(s) 261, 344, 1045, 1231, 1305
ApoI RAATTY 1 cut(s) 297
AspA2I CCTAGG 1 cut(s) 778
AspLEI GCGC 5 cut(s) 449, 1321, 1356, 1371, 1373
AspS9I GGNCC 2 cut(s) 1157, 1313
AsuC2I CCSGG 2 cut(s) 308, 331
AsuHPI GGTGA 3 cut(s) 887, 896, 1104
AsuII TTCGAA 1 cut(s) 859
AvaI CYCGRG 2 cut(s) 392, 1144
AvaII GGWCC 1 cut(s) 1313
AvrII CCTAGG 1 cut(s) 778
BanI GGYRCC 1 cut(s) 1387
BanII GRGCYC 1 cut(s) 393
BbsI GAAGAC 1 cut(s) 1467
BbvCI CCTCAGC 1 cut(s) 450
BbvI GCAGC 5 cut(s) 273, 356, 1032, 1218, 1292
BccI CCATC 4 cut(s) 407, 808, 1004, 1214
BcgI CGANNNNNNTGC 4 cut(s) 1065, 1099, 1298, 1332
BciT130I CCWGG 1 cut(s) 893
BclI TGATCA 1 cut(s) 1345
BcnI CCSGG 2 cut(s) 308, 331
BcuI ACTAGT 2 cut(s) 272, 424
BfaI CTAG 7 cut(s) 273, 425, 779, 824, 1130, 1424, 1437
BfuAI ACCTGC 1 cut(s) 1324
BisI GCNGC 6 cut(s) 262, 345, 1046, 1232, 1306, 1331
BlnI CCTAGG 1 cut(s) 778
BlsI GCNGC 6 cut(s) 263, 346, 1047, 1233, 1307, 1332
BmcAI AGTACT 1 cut(s) 226
Bme1390I CCNGG 3 cut(s) 308, 331, 893
Bme18I GGWCC 1 cut(s) 1313
BmeT110I CYCGRG 2 cut(s) 392, 1144
BmgT120I GGNCC 2 cut(s) 1157, 1313
BmiI GGNNCC 3 cut(s) 1314, 1379, 1389
BmrFI CCNGG 3 cut(s) 308, 331, 893
BmrI ACTGGG 1 cut(s) 608
BmsI GCATC 1 cut(s) 1213
BmuI ACTGGG 1 cut(s) 608
BpiI GAAGAC 1 cut(s) 1467
BplI GAGNNNNNCTC 2 cut(s) 1127, 1159
BpmI CTGGAG 2 cut(s) 146, 1025
Bpu10I CCTNAGC 1 cut(s) 450
Bpu14I TTCGAA 1 cut(s) 859
BpuMI CCSGG 2 cut(s) 308, 331
BsaBI GATNNNNATC 2 cut(s) 78, 999
BsaJI CCNNGG 2 cut(s) 393, 778
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 3 cut(s) 144, 738, 852
Bse118I RCCGGY 2 cut(s) 852, 1384
Bse1I ACTGG 4 cut(s) 262, 603, 691, 871
Bse3DI GCAATG 2 cut(s) 1233, 1503
Bse8I GATNNNNATC 2 cut(s) 78, 999
BseBI CCWGG 1 cut(s) 893
BseDI CCNNGG 2 cut(s) 393, 778
BseGI GGATG 3 cut(s) 86, 655, 1368
BseJI GATNNNNATC 2 cut(s) 78, 999
BseLI CCNNNNNNNGG 3 cut(s) 144, 738, 852
BseMI GCAATG 2 cut(s) 1233, 1503
BseMII CTCAG 3 cut(s) 441, 489, 654
BseNI ACTGG 4 cut(s) 262, 603, 691, 871
BsePI GCGCGC 1 cut(s) 1369
BseRI GAGGAG 2 cut(s) 1086, 1298
BseXI GCAGC 5 cut(s) 273, 356, 1032, 1218, 1292
BseYI CCCAGC 2 cut(s) 379, 1373
Bsh1236I CGCG 2 cut(s) 1321, 1371
BshFI GGCC 3 cut(s) 783, 989, 1159
BshNI GGYRCC 1 cut(s) 1387
BsiHKCI CYCGRG 2 cut(s) 392, 1144
BsiSI CCGG 4 cut(s) 307, 331, 853, 1385
BslFI GGGAC 3 cut(s) 541, 852, 1326
BslI CCNNNNNNNGG 3 cut(s) 144, 738, 852
BsmFI GGGAC 3 cut(s) 541, 852, 1326
BsmI GAATGC 1 cut(s) 1438
BsnI GGCC 3 cut(s) 783, 989, 1159
BsoBI CYCGRG 2 cut(s) 392, 1144
Bsp119I TTCGAA 1 cut(s) 859
Bsp1286I GDGCHC 1 cut(s) 393
Bsp143I GATC 3 cut(s) 73, 310, 1345
BspACI CCGC 2 cut(s) 138, 1330
BspANI GGCC 3 cut(s) 783, 989, 1159
BspCNI CTCAG 3 cut(s) 442, 490, 653
BspFNI CGCG 2 cut(s) 1321, 1371
BspLI GGNNCC 3 cut(s) 1314, 1379, 1389
BspMI ACCTGC 1 cut(s) 1324
BspPI GGATC 1 cut(s) 318
BspT104I TTCGAA 1 cut(s) 859
BspT107I GGYRCC 1 cut(s) 1387
BsrBI CCGCTC 1 cut(s) 1330
BsrDI GCAATG 2 cut(s) 1233, 1503
BsrFI RCCGGY 2 cut(s) 852, 1384
BsrI ACTGG 4 cut(s) 262, 603, 691, 871
BssAI RCCGGY 2 cut(s) 852, 1384
BssECI CCNNGG 2 cut(s) 393, 778
BssHII GCGCGC 1 cut(s) 1369
BssMI GATC 3 cut(s) 73, 310, 1345
BssNAI GTATAC 1 cut(s) 270
BssT1I CCWWGG 1 cut(s) 778
Bst1107I GTATAC 1 cut(s) 270
Bst2UI CCWGG 1 cut(s) 893
Bst4CI ACNGT 2 cut(s) 245, 578
BstBI TTCGAA 1 cut(s) 859
BstC8I GCNNGC 4 cut(s) 389, 829, 854, 1371
BstDEI CTNAG 5 cut(s) 450, 498, 503, 640, 1219
BstF5I GGATG 3 cut(s) 86, 655, 1368
BstFNI CGCG 2 cut(s) 1321, 1371
BstHHI GCGC 5 cut(s) 449, 1321, 1356, 1371, 1373
BstKTI GATC 3 cut(s) 76, 313, 1348
BstMBI GATC 3 cut(s) 73, 310, 1345
BstMWI GCNNNNNNNGC 3 cut(s) 261, 285, 1327
BstNI CCWGG 1 cut(s) 893
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 3 cut(s) 306, 329, 891
BstUI CGCG 2 cut(s) 1321, 1371
BstV1I GCAGC 5 cut(s) 273, 356, 1032, 1218, 1292
BstV2I GAAGAC 1 cut(s) 1467
BstXI CCANNNNNNTGG 1 cut(s) 524
BstZ17I GTATAC 1 cut(s) 270
BsuRI GGCC 3 cut(s) 783, 989, 1159
BtsCI GGATG 3 cut(s) 86, 655, 1368
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 5 cut(s) 250, 255, 256, 570, 596
BveI ACCTGC 1 cut(s) 1324
Cac8I GCNNGC 4 cut(s) 389, 829, 854, 1371
CfoI GCGC 5 cut(s) 449, 1321, 1356, 1371, 1373
Cfr10I RCCGGY 2 cut(s) 852, 1384
Cfr13I GGNCC 2 cut(s) 1157, 1313
Csp6I GTAC 1 cut(s) 225
CviQI GTAC 1 cut(s) 225
DdeI CTNAG 5 cut(s) 450, 498, 503, 640, 1219
DpnI GATC 3 cut(s) 75, 312, 1347
DpnII GATC 3 cut(s) 73, 310, 1345
Eco130I CCWWGG 1 cut(s) 778
Eco147I AGGCCT 1 cut(s) 783
Eco24I GRGCYC 1 cut(s) 393
Eco47I GGWCC 1 cut(s) 1313
Eco57I CTGAAG 3 cut(s) 468, 1004, 1415
Eco88I CYCGRG 2 cut(s) 392, 1144
EcoO109I RGGNCCY 1 cut(s) 1313
EcoRI GAATTC 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 891
EcoT14I CCWWGG 1 cut(s) 778
EcoT38I GRGCYC 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 778
FalI AAGNNNNNCTT 2 cut(s) 1457, 1489
FaqI GGGAC 3 cut(s) 541, 852, 1326
FbaI TGATCA 1 cut(s) 1345
FblI GTMKAC 1 cut(s) 269
Fnu4HI GCNGC 6 cut(s) 262, 345, 1046, 1232, 1306, 1331
FokI GGATG 3 cut(s) 73, 662, 1375
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 6 cut(s) 262, 345, 1046, 1232, 1306, 1331
FspBI CTAG 7 cut(s) 273, 425, 779, 824, 1130, 1424, 1437
GlaI GCGC 5 cut(s) 448, 1320, 1355, 1370, 1372
GluI GCNGC 6 cut(s) 262, 345, 1046, 1232, 1306, 1331
GsaI CCCAGC 2 cut(s) 383, 1377
GsuI CTGGAG 2 cut(s) 146, 1025
HaeIII GGCC 3 cut(s) 783, 989, 1159
HapII CCGG 4 cut(s) 307, 331, 853, 1385
HhaI GCGC 5 cut(s) 449, 1321, 1356, 1371, 1373
Hin6I GCGC 5 cut(s) 447, 1319, 1354, 1369, 1371
HinP1I GCGC 5 cut(s) 447, 1319, 1354, 1369, 1371
HindIII AAGCTT 2 cut(s) 191, 366
HinfI GANTC 6 cut(s) 121, 151, 212, 878, 1142, 1214
HpaII CCGG 4 cut(s) 307, 331, 853, 1385
HphI GGTGA 3 cut(s) 887, 896, 1104
Hpy166II GTNNAC 3 cut(s) 270, 683, 887
Hpy188I TCNGA 3 cut(s) 499, 643, 1280
Hpy188III TCNNGA 4 cut(s) 71, 125, 294, 467
Hpy8I GTNNAC 3 cut(s) 270, 683, 887
HpyAV CCTTC 7 cut(s) 110, 115, 120, 395, 640, 797, 979
HpyCH4III ACNGT 2 cut(s) 245, 578
HpyCH4V TGCA 6 cut(s) 22, 661, 926, 1048, 1226, 1508
HpyF10VI GCNNNNNNNGC 3 cut(s) 261, 285, 1327
HpyF3I CTNAG 5 cut(s) 450, 498, 503, 640, 1219
HspAI GCGC 5 cut(s) 447, 1319, 1354, 1369, 1371
KroI GCCGGC 1 cut(s) 852
KroNI GCCGGC 1 cut(s) 854
Ksp22I TGATCA 1 cut(s) 1345
Kzo9I GATC 3 cut(s) 73, 310, 1345
LmnI GCTCC 4 cut(s) 937, 1073, 1264, 1327
Lsp1109I GCAGC 5 cut(s) 273, 356, 1032, 1218, 1292
LweI GCATC 1 cut(s) 1213
MaeI CTAG 7 cut(s) 273, 425, 779, 824, 1130, 1424, 1437
MaeIII GTNAC 4 cut(s) 467, 613, 875, 1445
MalI GATC 3 cut(s) 75, 312, 1347
MbiI CCGCTC 1 cut(s) 1330
MboI GATC 3 cut(s) 73, 310, 1345
MboII GAAGA 8 cut(s) 209, 371, 374, 410, 1004, 1201, 1408, 1472
MfeI CAATTG 1 cut(s) 1452
MhlI GDGCHC 1 cut(s) 393
MluCI AATT 6 cut(s) 133, 229, 297, 302, 1195, 1452
MlyI GAGTC 4 cut(s) 115, 872, 1136, 1208
MmeI TCCRAC 1 cut(s) 1405
MroNI GCCGGC 1 cut(s) 852
MseI TTAA 2 cut(s) 1022, 1290
MslI CAYNNNNRTG 1 cut(s) 1454
MspA1I CMGCKG 2 cut(s) 140, 1045
MspI CCGG 4 cut(s) 307, 331, 853, 1385
MspR9I CCNGG 3 cut(s) 308, 331, 893
MunI CAATTG 1 cut(s) 1452
Mva1269I GAATGC 1 cut(s) 1438
MvaI CCWGG 1 cut(s) 893
MvnI CGCG 2 cut(s) 1321, 1371
MwoI GCNNNNNNNGC 3 cut(s) 261, 285, 1327
NaeI GCCGGC 1 cut(s) 854
NciI CCSGG 2 cut(s) 308, 331
NdeII GATC 3 cut(s) 73, 310, 1345
NgoMIV GCCGGC 1 cut(s) 852
NlaIV GGNNCC 3 cut(s) 1314, 1379, 1389
NmuCI GTSAC 2 cut(s) 467, 875
NspI RCATGY 1 cut(s) 268
NspV TTCGAA 1 cut(s) 859
OliI CACNNNNGTG 1 cut(s) 1454
PaeR7I CTCGAG 1 cut(s) 1144
PauI GCGCGC 1 cut(s) 1369
PceI AGGCCT 1 cut(s) 783
PcsI WCGNNNNNNNCGW 1 cut(s) 856
PctI GAATGC 1 cut(s) 1438
PdiI GCCGGC 1 cut(s) 854
PfeI GAWTC 2 cut(s) 151, 212
PfoI TCCNGGA 1 cut(s) 306
PkrI GCNGC 6 cut(s) 263, 346, 1047, 1233, 1307, 1332
PleI GAGTC 4 cut(s) 115, 872, 1136, 1208
PpsI GAGTC 4 cut(s) 115, 872, 1136, 1208
PpuMI RGGWCCY 1 cut(s) 1313
PsiI TTATAA 1 cut(s) 221
Psp5II RGGWCCY 1 cut(s) 1313
Psp6I CCWGG 1 cut(s) 891
PspFI CCCAGC 2 cut(s) 379, 1373
PspGI CCWGG 1 cut(s) 891
PspN4I GGNNCC 3 cut(s) 1314, 1379, 1389
PspPI GGNCC 2 cut(s) 1157, 1313
PspPPI RGGWCCY 1 cut(s) 1313
PspXI VCTCGAGB 1 cut(s) 1144
PteI GCGCGC 1 cut(s) 1369
PvuII CAGCTG 1 cut(s) 1045
RsaI GTAC 1 cut(s) 226
RsaNI GTAC 1 cut(s) 225
RseI CAYNNNNRTG 1 cut(s) 1454
SaqAI TTAA 2 cut(s) 1022, 1290
SatI GCNGC 6 cut(s) 262, 345, 1046, 1232, 1306, 1331
Sau3AI GATC 3 cut(s) 73, 310, 1345
Sau96I GGNCC 2 cut(s) 1157, 1313
ScaI AGTACT 1 cut(s) 226
SchI GAGTC 4 cut(s) 115, 872, 1136, 1208
ScrFI CCNGG 3 cut(s) 308, 331, 893
SduI GDGCHC 1 cut(s) 393
SfaNI GCATC 1 cut(s) 1213
Sfr274I CTCGAG 1 cut(s) 1144
SfuI TTCGAA 1 cut(s) 859
SinI GGWCC 1 cut(s) 1313
SlaI CTCGAG 1 cut(s) 1144
SmiMI CAYNNNNRTG 1 cut(s) 1454
SmlI CTYRAG 1 cut(s) 1144
SmoI CTYRAG 1 cut(s) 1144
SpeI ACTAGT 2 cut(s) 272, 424
Sse9I AATT 6 cut(s) 133, 229, 297, 302, 1195, 1452
SseBI AGGCCT 1 cut(s) 783
SsiI CCGC 2 cut(s) 138, 1330
SspI AATATT 1 cut(s) 723
SspMI CTAG 7 cut(s) 273, 425, 779, 824, 1130, 1424, 1437
StuI AGGCCT 1 cut(s) 783
StyD4I CCNGG 3 cut(s) 306, 329, 891
StyI CCWWGG 1 cut(s) 778
TaaI ACNGT 2 cut(s) 245, 578
TaqI TCGA 5 cut(s) 119, 859, 1145, 1212, 1493
TasI AATT 6 cut(s) 133, 229, 297, 302, 1195, 1452
TatI WGTACW 1 cut(s) 224
TauI GCSGC 1 cut(s) 1333
TfiI GAWTC 2 cut(s) 151, 212
Tru1I TTAA 2 cut(s) 1022, 1290
Tru9I TTAA 2 cut(s) 1022, 1290
TscAI CASTG 5 cut(s) 250, 256, 262, 577, 603
TseFI GTSAC 2 cut(s) 467, 875
TseI GCWGC 5 cut(s) 261, 344, 1045, 1231, 1305
Tsp45I GTSAC 2 cut(s) 467, 875
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 5 cut(s) 250, 256, 262, 577, 603
VpaK11BI GGWCC 1 cut(s) 1313
XapI RAATTY 1 cut(s) 297
XceI RCATGY 1 cut(s) 268
XhoI CTCGAG 1 cut(s) 1144
XmaJI CCTAGG 1 cut(s) 778
XmiI GTMKAC 1 cut(s) 269
XspI CTAG 7 cut(s) 273, 425, 779, 824, 1130, 1424, 1437
ZrmI AGTACT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.