RLG00000006877

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0017652
Physical Location & Seq
Reverse (-)
11382887 .. 11384407
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006877

Sequence Viewer

Length: 1521 bp
ATGGCATCTGGTTTCTATGTGCAACCTTGTGAAGTCTACGGCAATAACAAATGTGATGAGGTTATTGGTCTTGATCTCAATCTCTCATCCATGTCATGCCTTCCCTTCCCTTCTCTTTCGACTCTGGAGAACAATTCTGCTGTCTGGGAGATTCCGTTCATAGATGTAACAAGAAATCATAAGAGGGTAAAGCTTGAAGATAGTGCCGATGAATCCATTATAAGTACTAATTATAGTCTTTACAGTGGCAGCATGTATACTAGTGGCTTCAATAGCTTGTCAAGAATTCAATTCCGGGATCACATATCAGCTTACACCCGGAGATACTTGGCAGCAGAGAAAATGGAAGAAGCAGCTTCAACCTTGCTGGGAGGCGAGCCCGAGGAAGAAGGAAGTGGAGATGGAATGAAACTAGTTCAAAAGCTCATTTCGTGCGCTGAGGCTGTGGCTTGTCGAGACAAAACTCGTGCTTCAGCATTGCTTTCTGAGCTTAGAACTAATGCCCAGGTCTTTGGGACATCATTCCAGCGAGTAGCTTCCTGCTTTATCCAAGCTCTCTCCGACCGTCTTGCTCTCGTTCAACCACTGGGGGCTGTGGGAGTTACAACAAAGATGATTGCTGTTTCATCAGAGAAGGATGAGGCTTTGCACCTTGTTTATGAGATTTGCCCACATATCCAGTTTAGTCATTTTGTAGCAAACACATCAATATTGGAAGCCTTTGAGGGGGAGAGCTATGTTCATGTCATAGACTTGGGCATGACCCTAGGCCTCCTTCATGGCTACCAATGGCTCAACTTGATGGAAAGTCTAGCCAGCCGAACAGGACAACCCTTACGCCGGCTTCGAATAACTGGTGTTGGTGACTCTGGTGAACGCCTTGAAGCGATTGGTAATGAACTAAAGTTGTATGCACAAAGCATGGAGCTGGATTTTGGATTTTTAGCAGTGGAAAGCAACTTGGAAAACCTGAAGGCCGAAGATTTCAATCTCATTGATGGGGAGGTTTTAGTCATCAATAGCATACTCCAGCTGCATTGTCTTGTGAAAGAGAGTAGAGGAGCATTGAACTCGGTTCTACAAACACTATACGACCTATCACCAAAGCTAATGGTTCTAGTTGAGCAGGACTCGAGCCACAATGGGCCTTTCTTTCTTGGGAGGTTCATGGAGGCATTACATAATTACTCTGCCATCTTCGACTCCTTAGATGCAATGCTGCCCAGATATGACACAAGGAGAGCTAGAATGGAGCAGTTTTATTTTGGAGAGGAGATTAAGAACATAGTAAGCTGCGAGGGACCTGCGCGAGTGGAGCGGCACGAAAGAGTTGATAAATGGCGCAGGAGGATGAGGCGCGCTGGGTTCCAACCGGCACCCCTGAAGATGATTACGCAGGCTACAAAATGGCTAGAAATGAATGCTAGTGAGGGTTACACAATTGTGGAAGACAAGGGTTGCTTTGTTCTTGGGTGGAAGTCGAAGCCTATCATTGCAGCTTCTTGCTGGAGATGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

507

Amino Acids

56.72

Weight (kDa)

5.46

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 138 - 504 5e-111 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017652)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24820
malus_domestica MD13G1108700.v1.1
prunus_persica Prupe.1G237500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0431901
rosa_laevigata RLG00000006877
rosa_roxburghii Rroxscaffold_5G00373330
rosa_rugosa Rorug04G0252900
rosa_samantha Rh4AG310100 Rh4BG317200 Rh4CG332500 Rh4DG312100
rosa_wichuraiana Rw4G026980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 221
Acc36I ACCTGC 1 cut(s) 1312
AccB1I GGYRCC 1 cut(s) 1375
AccBSI CCGCTC 1 cut(s) 1318
AccI GTMKAC 2 cut(s) 36, 257
AccII CGCG 2 cut(s) 1309, 1359
AciI CCGC 1 cut(s) 1318
AclWI GGATC 1 cut(s) 306
AcsI RAATTY 1 cut(s) 285
AcuI CTGAAG 3 cut(s) 456, 992, 1403
AfaI GTAC 1 cut(s) 226
AfiI CCNNNNNNNGG 2 cut(s) 726, 840
AgsI TTSAA 9 cut(s) 197, 271, 290, 360, 419, 581, 884, 988, 1069
AhlI ACTAGT 2 cut(s) 260, 412
AjnI CCWGG 1 cut(s) 504
AleI CACNNNNGTG 1 cut(s) 1442
AloI GAACNNNNNNTCC 2 cut(s) 140, 172
Alw26I GTCTC 1 cut(s) 450
AlwI GGATC 1 cut(s) 306
Ama87I CYCGRG 2 cut(s) 380, 1132
AoxI GGCC 3 cut(s) 769, 975, 1145
ApeKI GCWGC 7 cut(s) 249, 332, 353, 1033, 1219, 1293, 1496
ApoI RAATTY 1 cut(s) 285
AspA2I CCTAGG 1 cut(s) 766
AspLEI GCGC 5 cut(s) 437, 1309, 1344, 1359, 1361
AspS9I GGNCC 2 cut(s) 1145, 1301
AsuC2I CCSGG 2 cut(s) 296, 319
AsuHPI GGTGA 3 cut(s) 875, 884, 1092
AsuII TTCGAA 1 cut(s) 847
AvaI CYCGRG 2 cut(s) 380, 1132
AvaII GGWCC 1 cut(s) 1301
AvrII CCTAGG 1 cut(s) 766
BanI GGYRCC 1 cut(s) 1375
BanII GRGCYC 1 cut(s) 381
BauI CACGAG 1 cut(s) 465
BbsI GAAGAC 1 cut(s) 1455
BbvCI CCTCAGC 1 cut(s) 438
BbvI GCAGC 7 cut(s) 261, 344, 365, 1020, 1206, 1280, 1508
BccI CCATC 4 cut(s) 395, 796, 992, 1202
BceAI ACGGC 1 cut(s) 55
BcgI CGANNNNNNTGC 6 cut(s) 551, 585, 1053, 1087, 1286, 1320
BciT130I CCWGG 1 cut(s) 506
BcnI CCSGG 2 cut(s) 296, 319
BcoDI GTCTC 1 cut(s) 450
BcuI ACTAGT 2 cut(s) 260, 412
BfaI CTAG 8 cut(s) 261, 413, 767, 812, 1118, 1245, 1412, 1425
BfuAI ACCTGC 1 cut(s) 1312
BisI GCNGC 8 cut(s) 250, 333, 354, 1034, 1220, 1294, 1319, 1497
BlnI CCTAGG 1 cut(s) 766
BlsI GCNGC 8 cut(s) 251, 334, 355, 1035, 1221, 1295, 1320, 1498
BmcAI AGTACT 1 cut(s) 226
Bme1390I CCNGG 3 cut(s) 296, 319, 506
Bme18I GGWCC 1 cut(s) 1301
BmeT110I CYCGRG 2 cut(s) 380, 1132
BmgT120I GGNCC 2 cut(s) 1145, 1301
BmiI GGNNCC 3 cut(s) 1302, 1367, 1377
BmrFI CCNGG 3 cut(s) 296, 319, 506
BmrI ACTGGG 1 cut(s) 596
BmsI GCATC 2 cut(s) 14, 1201
BmuI ACTGGG 1 cut(s) 596
BpiI GAAGAC 1 cut(s) 1455
BplI GAGNNNNNCTC 2 cut(s) 1115, 1147
BpmI CTGGAG 2 cut(s) 146, 1013
Bpu10I CCTNAGC 1 cut(s) 438
Bpu14I TTCGAA 1 cut(s) 847
BpuMI CCSGG 2 cut(s) 296, 319
BsaBI GATNNNNATC 2 cut(s) 78, 987
BsaJI CCNNGG 3 cut(s) 381, 504, 766
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 2 cut(s) 726, 840
Bse118I RCCGGY 2 cut(s) 840, 1372
Bse1I ACTGG 3 cut(s) 591, 679, 859
Bse3DI GCAATG 3 cut(s) 476, 1221, 1491
Bse8I GATNNNNATC 2 cut(s) 78, 987
BseBI CCWGG 1 cut(s) 506
BseDI CCNNGG 3 cut(s) 381, 504, 766
BseGI GGATG 3 cut(s) 86, 643, 1356
BseJI GATNNNNATC 2 cut(s) 78, 987
BseLI CCNNNNNNNGG 2 cut(s) 726, 840
BseMI GCAATG 3 cut(s) 476, 1221, 1491
BseMII CTCAG 2 cut(s) 429, 477
BseNI ACTGG 3 cut(s) 591, 679, 859
BsePI GCGCGC 1 cut(s) 1357
BseRI GAGGAG 2 cut(s) 1074, 1286
BseXI GCAGC 7 cut(s) 261, 344, 365, 1020, 1206, 1280, 1508
BseYI CCCAGC 2 cut(s) 367, 1361
Bsh1236I CGCG 2 cut(s) 1309, 1359
Bsh1285I CGRYCG 1 cut(s) 565
BshFI GGCC 3 cut(s) 771, 977, 1147
BshNI GGYRCC 1 cut(s) 1375
BsiEI CGRYCG 1 cut(s) 565
BsiHKCI CYCGRG 2 cut(s) 380, 1132
BsiSI CCGG 4 cut(s) 295, 319, 841, 1373
BslFI GGGAC 2 cut(s) 529, 1314
BslI CCNNNNNNNGG 2 cut(s) 726, 840
BsmAI GTCTC 1 cut(s) 450
BsmFI GGGAC 2 cut(s) 529, 1314
BsmI GAATGC 1 cut(s) 1426
BsnI GGCC 3 cut(s) 771, 977, 1147
BsoBI CYCGRG 2 cut(s) 380, 1132
Bsp119I TTCGAA 1 cut(s) 847
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 2 cut(s) 73, 298
BspACI CCGC 1 cut(s) 1318
BspANI GGCC 3 cut(s) 771, 977, 1147
BspCNI CTCAG 2 cut(s) 430, 478
BspFNI CGCG 2 cut(s) 1309, 1359
BspLI GGNNCC 3 cut(s) 1302, 1367, 1377
BspMI ACCTGC 1 cut(s) 1312
BspPI GGATC 1 cut(s) 306
BspT104I TTCGAA 1 cut(s) 847
BspT107I GGYRCC 1 cut(s) 1375
BsrBI CCGCTC 1 cut(s) 1318
BsrDI GCAATG 3 cut(s) 476, 1221, 1491
BsrFI RCCGGY 2 cut(s) 840, 1372
BsrI ACTGG 3 cut(s) 591, 679, 859
BssAI RCCGGY 2 cut(s) 840, 1372
BssECI CCNNGG 3 cut(s) 381, 504, 766
BssHII GCGCGC 1 cut(s) 1357
BssMI GATC 2 cut(s) 73, 298
BssNAI GTATAC 1 cut(s) 258
BssSI CACGAG 1 cut(s) 465
BssT1I CCWWGG 1 cut(s) 766
Bst1107I GTATAC 1 cut(s) 258
Bst2BI CACGAG 1 cut(s) 465
Bst2UI CCWGG 1 cut(s) 506
Bst4CI ACNGT 2 cut(s) 245, 566
BstBI TTCGAA 1 cut(s) 847
BstC8I GCNNGC 5 cut(s) 377, 817, 842, 1359, 1398
BstDEI CTNAG 4 cut(s) 438, 486, 491, 1207
BstF5I GGATG 3 cut(s) 86, 643, 1356
BstFNI CGCG 2 cut(s) 1309, 1359
BstHHI GCGC 5 cut(s) 437, 1309, 1344, 1359, 1361
BstKTI GATC 2 cut(s) 76, 301
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 2 cut(s) 73, 298
BstMCI CGRYCG 1 cut(s) 565
BstMWI GCNNNNNNNGC 3 cut(s) 273, 487, 1315
BstNI CCWGG 1 cut(s) 506
BstNSI RCATGY 1 cut(s) 256
BstSCI CCNGG 3 cut(s) 294, 317, 504
BstUI CGCG 2 cut(s) 1309, 1359
BstV1I GCAGC 7 cut(s) 261, 344, 365, 1020, 1206, 1280, 1508
BstV2I GAAGAC 1 cut(s) 1455
BstXI CCANNNNNNTGG 1 cut(s) 512
BstZ17I GTATAC 1 cut(s) 258
BsuRI GGCC 3 cut(s) 771, 977, 1147
BtsCI GGATG 3 cut(s) 86, 643, 1356
BtsI GCAGTG 1 cut(s) 954
BtsIMutI CAGTG 3 cut(s) 250, 584, 954
BveI ACCTGC 1 cut(s) 1312
Cac8I GCNNGC 5 cut(s) 377, 817, 842, 1359, 1398
CfoI GCGC 5 cut(s) 437, 1309, 1344, 1359, 1361
Cfr10I RCCGGY 2 cut(s) 840, 1372
Cfr13I GGNCC 2 cut(s) 1145, 1301
Csp6I GTAC 1 cut(s) 225
CviAII CATG 8 cut(s) 91, 96, 253, 743, 760, 779, 922, 1168
CviQI GTAC 1 cut(s) 225
DdeI CTNAG 4 cut(s) 438, 486, 491, 1207
DpnI GATC 2 cut(s) 75, 300
DpnII GATC 2 cut(s) 73, 298
Eco130I CCWWGG 1 cut(s) 766
Eco147I AGGCCT 1 cut(s) 771
Eco24I GRGCYC 1 cut(s) 381
Eco47I GGWCC 1 cut(s) 1301
Eco57I CTGAAG 3 cut(s) 456, 992, 1403
Eco88I CYCGRG 2 cut(s) 380, 1132
EcoO109I RGGNCCY 1 cut(s) 1301
EcoRI GAATTC 1 cut(s) 285
EcoRII CCWGG 1 cut(s) 504
EcoT14I CCWWGG 1 cut(s) 766
EcoT38I GRGCYC 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 766
FaeI CATG 8 cut(s) 94, 99, 256, 746, 763, 782, 925, 1171
FalI AAGNNNNNCTT 2 cut(s) 1445, 1477
FaqI GGGAC 2 cut(s) 529, 1314
FatI CATG 8 cut(s) 90, 95, 252, 742, 759, 778, 921, 1167
FblI GTMKAC 2 cut(s) 36, 257
Fnu4HI GCNGC 8 cut(s) 250, 333, 354, 1034, 1220, 1294, 1319, 1497
FokI GGATG 3 cut(s) 73, 650, 1363
FriOI GRGCYC 1 cut(s) 381
Fsp4HI GCNGC 8 cut(s) 250, 333, 354, 1034, 1220, 1294, 1319, 1497
FspBI CTAG 8 cut(s) 261, 413, 767, 812, 1118, 1245, 1412, 1425
GlaI GCGC 5 cut(s) 436, 1308, 1343, 1358, 1360
GluI GCNGC 8 cut(s) 250, 333, 354, 1034, 1220, 1294, 1319, 1497
GsaI CCCAGC 2 cut(s) 371, 1365
GsuI CTGGAG 2 cut(s) 146, 1013
HaeIII GGCC 3 cut(s) 771, 977, 1147
HapII CCGG 4 cut(s) 295, 319, 841, 1373
HhaI GCGC 5 cut(s) 437, 1309, 1344, 1359, 1361
Hin1II CATG 8 cut(s) 94, 99, 256, 746, 763, 782, 925, 1171
Hin6I GCGC 5 cut(s) 435, 1307, 1342, 1357, 1359
HinP1I GCGC 5 cut(s) 435, 1307, 1342, 1357, 1359
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 6 cut(s) 121, 151, 212, 866, 1130, 1202
HpaII CCGG 4 cut(s) 295, 319, 841, 1373
HphI GGTGA 3 cut(s) 875, 884, 1092
Hpy166II GTNNAC 3 cut(s) 37, 258, 875
Hpy188I TCNGA 3 cut(s) 487, 562, 631
Hpy188III TCNNGA 4 cut(s) 71, 125, 282, 455
Hpy8I GTNNAC 3 cut(s) 37, 258, 875
HpyAV CCTTC 7 cut(s) 110, 115, 120, 383, 628, 785, 967
HpyCH4III ACNGT 2 cut(s) 245, 566
HpyCH4V TGCA 6 cut(s) 22, 649, 914, 1036, 1214, 1496
HpyF10VI GCNNNNNNNGC 3 cut(s) 273, 487, 1315
HpyF3I CTNAG 4 cut(s) 438, 486, 491, 1207
Hsp92II CATG 8 cut(s) 94, 99, 256, 746, 763, 782, 925, 1171
HspAI GCGC 5 cut(s) 435, 1307, 1342, 1357, 1359
KroI GCCGGC 1 cut(s) 840
KroNI GCCGGC 1 cut(s) 842
Kzo9I GATC 2 cut(s) 73, 298
LmnI GCTCC 4 cut(s) 925, 1061, 1252, 1315
Lsp1109I GCAGC 7 cut(s) 261, 344, 365, 1020, 1206, 1280, 1508
LweI GCATC 2 cut(s) 14, 1201
MaeI CTAG 8 cut(s) 261, 413, 767, 812, 1118, 1245, 1412, 1425
MaeIII GTNAC 4 cut(s) 166, 601, 863, 1433
MalI GATC 2 cut(s) 75, 300
MbiI CCGCTC 1 cut(s) 1318
MboI GATC 2 cut(s) 73, 298
MboII GAAGA 7 cut(s) 209, 359, 398, 992, 1189, 1396, 1460
MfeI CAATTG 1 cut(s) 1440
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 6 cut(s) 133, 229, 285, 290, 1183, 1440
MlyI GAGTC 4 cut(s) 115, 860, 1124, 1196
MmeI TCCRAC 2 cut(s) 585, 1393
MroNI GCCGGC 1 cut(s) 840
MseI TTAA 1 cut(s) 1278
MslI CAYNNNNRTG 1 cut(s) 1442
MspA1I CMGCKG 1 cut(s) 1033
MspI CCGG 4 cut(s) 295, 319, 841, 1373
MspR9I CCNGG 3 cut(s) 296, 319, 506
MunI CAATTG 1 cut(s) 1440
Mva1269I GAATGC 1 cut(s) 1426
MvaI CCWGG 1 cut(s) 506
MvnI CGCG 2 cut(s) 1309, 1359
MwoI GCNNNNNNNGC 3 cut(s) 273, 487, 1315
NaeI GCCGGC 1 cut(s) 842
NciI CCSGG 2 cut(s) 296, 319
NdeII GATC 2 cut(s) 73, 298
NgoMIV GCCGGC 1 cut(s) 840
NlaIII CATG 8 cut(s) 94, 99, 256, 746, 763, 782, 925, 1171
NlaIV GGNNCC 3 cut(s) 1302, 1367, 1377
NmuCI GTSAC 1 cut(s) 863
NspI RCATGY 1 cut(s) 256
NspV TTCGAA 1 cut(s) 847
OliI CACNNNNGTG 1 cut(s) 1442
PaeR7I CTCGAG 1 cut(s) 1132
PauI GCGCGC 1 cut(s) 1357
PceI AGGCCT 1 cut(s) 771
PcsI WCGNNNNNNNCGW 1 cut(s) 844
PctI GAATGC 1 cut(s) 1426
PdiI GCCGGC 1 cut(s) 842
PfeI GAWTC 2 cut(s) 151, 212
PfoI TCCNGGA 1 cut(s) 294
PkrI GCNGC 8 cut(s) 251, 334, 355, 1035, 1221, 1295, 1320, 1498
PleI GAGTC 4 cut(s) 115, 860, 1124, 1196
PpsI GAGTC 4 cut(s) 115, 860, 1124, 1196
PpuMI RGGWCCY 1 cut(s) 1301
PsiI TTATAA 1 cut(s) 221
Psp5II RGGWCCY 1 cut(s) 1301
Psp6I CCWGG 1 cut(s) 504
PspFI CCCAGC 2 cut(s) 367, 1361
PspGI CCWGG 1 cut(s) 504
PspN4I GGNNCC 3 cut(s) 1302, 1367, 1377
PspPI GGNCC 2 cut(s) 1145, 1301
PspPPI RGGWCCY 1 cut(s) 1301
PspXI VCTCGAGB 1 cut(s) 1132
PteI GCGCGC 1 cut(s) 1357
PvuII CAGCTG 1 cut(s) 1033
RsaI GTAC 1 cut(s) 226
RsaNI GTAC 1 cut(s) 225
RseI CAYNNNNRTG 1 cut(s) 1442
SaqAI TTAA 1 cut(s) 1278
SatI GCNGC 8 cut(s) 250, 333, 354, 1034, 1220, 1294, 1319, 1497
Sau3AI GATC 2 cut(s) 73, 298
Sau96I GGNCC 2 cut(s) 1145, 1301
ScaI AGTACT 1 cut(s) 226
SchI GAGTC 4 cut(s) 115, 860, 1124, 1196
ScrFI CCNGG 3 cut(s) 296, 319, 506
SduI GDGCHC 1 cut(s) 381
SfaNI GCATC 2 cut(s) 14, 1201
Sfr274I CTCGAG 1 cut(s) 1132
SfuI TTCGAA 1 cut(s) 847
SinI GGWCC 1 cut(s) 1301
SlaI CTCGAG 1 cut(s) 1132
SmiMI CAYNNNNRTG 1 cut(s) 1442
SmlI CTYRAG 1 cut(s) 1132
SmoI CTYRAG 1 cut(s) 1132
SpeI ACTAGT 2 cut(s) 260, 412
Sse9I AATT 6 cut(s) 133, 229, 285, 290, 1183, 1440
SseBI AGGCCT 1 cut(s) 771
SsiI CCGC 1 cut(s) 1318
SspI AATATT 1 cut(s) 711
SspMI CTAG 8 cut(s) 261, 413, 767, 812, 1118, 1245, 1412, 1425
StuI AGGCCT 1 cut(s) 771
StyD4I CCNGG 3 cut(s) 294, 317, 504
StyI CCWWGG 1 cut(s) 766
TaaI ACNGT 2 cut(s) 245, 566
TaqI TCGA 6 cut(s) 119, 454, 847, 1133, 1200, 1481
TasI AATT 6 cut(s) 133, 229, 285, 290, 1183, 1440
TatI WGTACW 1 cut(s) 224
TauI GCSGC 1 cut(s) 1321
TfiI GAWTC 2 cut(s) 151, 212
Tru1I TTAA 1 cut(s) 1278
Tru9I TTAA 1 cut(s) 1278
TscAI CASTG 3 cut(s) 250, 591, 954
TseFI GTSAC 1 cut(s) 863
TseI GCWGC 7 cut(s) 249, 332, 353, 1033, 1219, 1293, 1496
Tsp45I GTSAC 1 cut(s) 863
TspDTI ATGAA 9 cut(s) 148, 225, 422, 615, 731, 767, 912, 1156, 1433
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 3 cut(s) 250, 591, 954
VpaK11BI GGWCC 1 cut(s) 1301
XapI RAATTY 1 cut(s) 285
XceI RCATGY 1 cut(s) 256
XhoI CTCGAG 1 cut(s) 1132
XmaJI CCTAGG 1 cut(s) 766
XmiI GTMKAC 2 cut(s) 36, 257
XspI CTAG 8 cut(s) 261, 413, 767, 812, 1118, 1245, 1412, 1425
ZrmI AGTACT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.