FvH4_4g28220

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014445
Physical Location & Seq
Reverse (-)
28910000 .. 28913003
3004 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g28220.t2

Sequence Viewer

Length: 756 bp
ATGGCTTTACTCAAAATGGGTTCACTAGTCCCTCTGCTCTTGCTTGCTAGAATCTCTGGGCTTTTGGTGGCAGTGCTGGTCTTGATTTGGACTTTTTCATTCAGGTCCAGCTTTCATCATCACTCTACTGATGAGTCTTTTGACTCTTTTCCCCAACAAGACCAAGGGATCACCTATTCAGCTCTTCATCCTTTGTTCATGGTCATTGGCTTTATTCTCATCAGTGGAGAAGCAATTTTGATACATAGGTGGTTGCCTGGTTCAAGAAGTTTGAAGAAATCAGTGCATCTGTGTCTTCAAGGTGTGGCTTTGTGTTCTGGGATCTTTGGGATCTGGACAAAGTTTCAGGGCCAGAGGGGTGTTGTGGCTAACTTCTATAGTCTTCATTCTTGGATGGGTTTGGTCTGCATTATCTTGTTTGGAGCTCAGTGGGTGATGGGTTTCTTGAGCTTTTGGCATAGAGGGGAAGCGCGCACAGTAAGGCTAAGGGTATTGCCTTGGCACATCTTCCTGGGGCTCTACACATATGGTTTGGCAGTGGCTACTGCAGAAACTGGGCTTCTGGAGAAGTTGACATTCTTGCAAACAAGGAGGAATGTGTCTAAACACTGCCCAGAGTCCATGGTTGTTAATAGTTTAGGGCTTGGGTTGGCCTTGCTCAGTGGCATTGTAATATTGGCTGCAATATCACCCAAGTACCAACCCACTCAGACTAAACTAATTTACTCAGATACCAAAAAATGTCCCTCTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

252

Amino Acids

27.92

Weight (kDa)

9.66

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 1 - 127 7.5e-38 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014445)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26100
fragaria_vesca FvH4_4g28220 FvH4_4g28220 FvH4_4g28220 FvH4_4g28220
malus_domestica MD13G1048100.v1.1
prunus_persica Prupe.1G304000_v2.0.a1
pyrus_communis pycom13g04220
rosa_chinensis RchiOBHm_Chr4g0436011
rosa_laevigata RLG00000006542
rosa_roxburghii Rroxscaffold_5G00377160
rosa_rugosa Rorug04G0291200
rosa_samantha Rh4AG345500 Rh4BG354500 Rh4CG369000 Rh4DG348200
rosa_wichuraiana Rw4G030210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 472
AclWI GGATC 3 cut(s) 176, 329, 338
AfaI GTAC 1 cut(s) 698
AgsI TTSAA 3 cut(s) 264, 274, 299
AhlI ACTAGT 1 cut(s) 25
AjnI CCWGG 2 cut(s) 256, 510
AluBI AGCT 4 cut(s) 111, 182, 425, 450
AluI AGCT 4 cut(s) 111, 182, 425, 450
Alw21I GWGCWC 1 cut(s) 427
AlwI GGATC 3 cut(s) 176, 329, 338
AlwNI CAGNNNCTG 1 cut(s) 554
AoxI GGCC 2 cut(s) 349, 651
ApeKI GCWGC 1 cut(s) 680
AspLEI GCGC 2 cut(s) 472, 474
AspS9I GGNCC 2 cut(s) 105, 349
AsuHPI GGTGA 3 cut(s) 163, 445, 681
AvaII GGWCC 1 cut(s) 105
BaeI ACNNNNGTAYC 4 cut(s) 233, 266, 680, 713
BanII GRGCYC 2 cut(s) 427, 519
BbsI GAAGAC 2 cut(s) 287, 374
Bbv12I GWGCWC 1 cut(s) 427
BbvI GCAGC 1 cut(s) 667
BccI CCATC 2 cut(s) 388, 430
BciT130I CCWGG 2 cut(s) 258, 512
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 2 cut(s) 26, 48
BfmI CTRYAG 2 cut(s) 376, 546
BisI GCNGC 1 cut(s) 681
BlsI GCNGC 1 cut(s) 682
Bme1390I CCNGG 2 cut(s) 258, 512
Bme18I GGWCC 1 cut(s) 105
BmgT120I GGNCC 2 cut(s) 105, 349
BmrFI CCNGG 2 cut(s) 258, 512
BmrI ACTGGG 1 cut(s) 564
BmsI GCATC 1 cut(s) 295
BmuI ACTGGG 1 cut(s) 564
BpiI GAAGAC 2 cut(s) 287, 374
BpmI CTGGAG 1 cut(s) 584
Bpu10I CCTNAGC 1 cut(s) 485
BpuEI CTTGAG 1 cut(s) 466
BsaJI CCNNGG 4 cut(s) 163, 497, 511, 621
Bse1I ACTGG 1 cut(s) 559
BseBI CCWGG 2 cut(s) 258, 512
BseDI CCNNGG 4 cut(s) 163, 497, 511, 621
BseGI GGATG 2 cut(s) 187, 399
BseMII CTCAG 4 cut(s) 440, 673, 722, 741
BseNI ACTGG 1 cut(s) 559
BsePI GCGCGC 1 cut(s) 470
BseXI GCAGC 1 cut(s) 667
Bsh1236I CGCG 1 cut(s) 472
BshFI GGCC 2 cut(s) 351, 653
BsiHKAI GWGCWC 1 cut(s) 427
BslFI GGGAC 2 cut(s) 14, 729
BsmFI GGGAC 2 cut(s) 14, 729
BsnI GGCC 2 cut(s) 351, 653
Bsp1286I GDGCHC 2 cut(s) 427, 519
Bsp143I GATC 3 cut(s) 168, 321, 330
Bsp19I CCATGG 1 cut(s) 621
BspANI GGCC 2 cut(s) 351, 653
BspCNI CTCAG 4 cut(s) 439, 672, 721, 740
BspFNI CGCG 1 cut(s) 472
BspMAI CTGCAG 1 cut(s) 550
BspPI GGATC 3 cut(s) 176, 329, 338
BspQI GCTCTTC 1 cut(s) 189
BsrI ACTGG 1 cut(s) 559
BssECI CCNNGG 4 cut(s) 163, 497, 511, 621
BssHII GCGCGC 1 cut(s) 470
BssMI GATC 3 cut(s) 168, 321, 330
BssT1I CCWWGG 3 cut(s) 163, 497, 621
Bst2UI CCWGG 2 cut(s) 258, 512
Bst4CI ACNGT 1 cut(s) 478
Bst6I CTCTTC 1 cut(s) 189
BstC8I GCNNGC 2 cut(s) 45, 472
BstDEI CTNAG 5 cut(s) 426, 485, 659, 708, 727
BstDSI CCRYGG 1 cut(s) 621
BstF5I GGATG 2 cut(s) 187, 399
BstFNI CGCG 1 cut(s) 472
BstHHI GCGC 2 cut(s) 472, 474
BstKTI GATC 3 cut(s) 171, 324, 333
BstMBI GATC 3 cut(s) 168, 321, 330
BstNI CCWGG 2 cut(s) 258, 512
BstSCI CCNGG 2 cut(s) 256, 510
BstSFI CTRYAG 2 cut(s) 376, 546
BstUI CGCG 1 cut(s) 472
BstV1I GCAGC 1 cut(s) 667
BstV2I GAAGAC 2 cut(s) 287, 374
BstX2I RGATCY 2 cut(s) 321, 330
BstYI RGATCY 2 cut(s) 321, 330
BsuRI GGCC 2 cut(s) 351, 653
BtgI CCRYGG 1 cut(s) 621
BtsCI GGATG 2 cut(s) 187, 399
BtsI GCAGTG 3 cut(s) 78, 543, 607
BtsIMutI CAGTG 7 cut(s) 78, 229, 288, 434, 543, 607, 667
Cac8I GCNNGC 2 cut(s) 45, 472
CaiI CAGNNNCTG 1 cut(s) 554
CfoI GCGC 2 cut(s) 472, 474
Cfr13I GGNCC 2 cut(s) 105, 349
Csp6I GTAC 1 cut(s) 697
CviAII CATG 2 cut(s) 199, 622
CviQI GTAC 1 cut(s) 697
DdeI CTNAG 5 cut(s) 426, 485, 659, 708, 727
DpnI GATC 3 cut(s) 170, 323, 332
DpnII GATC 3 cut(s) 168, 321, 330
Eam1104I CTCTTC 1 cut(s) 189
EarI CTCTTC 1 cut(s) 189
Ecl136II GAGCTC 1 cut(s) 425
Eco130I CCWWGG 3 cut(s) 163, 497, 621
Eco24I GRGCYC 2 cut(s) 427, 519
Eco47I GGWCC 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 425
EcoICRI GAGCTC 1 cut(s) 425
EcoRII CCWGG 2 cut(s) 256, 510
EcoT14I CCWWGG 3 cut(s) 163, 497, 621
EcoT38I GRGCYC 2 cut(s) 427, 519
ErhI CCWWGG 3 cut(s) 163, 497, 621
FaeI CATG 2 cut(s) 202, 625
FaiI YATR 7 cut(s) 200, 246, 378, 459, 526, 528, 623
FaqI GGGAC 2 cut(s) 14, 729
FatI CATG 2 cut(s) 198, 621
FauNDI CATATG 1 cut(s) 526
Fnu4HI GCNGC 1 cut(s) 681
FokI GGATG 2 cut(s) 174, 406
FriOI GRGCYC 2 cut(s) 427, 519
Fsp4HI GCNGC 1 cut(s) 681
FspBI CTAG 2 cut(s) 26, 48
GlaI GCGC 2 cut(s) 471, 473
GluI GCNGC 1 cut(s) 681
GsuI CTGGAG 1 cut(s) 584
HaeIII GGCC 2 cut(s) 351, 653
HhaI GCGC 2 cut(s) 472, 474
Hin1II CATG 2 cut(s) 202, 625
Hin6I GCGC 2 cut(s) 470, 472
HinP1I GCGC 2 cut(s) 470, 472
HincII GTYRAC 1 cut(s) 573
HindII GTYRAC 1 cut(s) 573
HinfI GANTC 4 cut(s) 51, 134, 143, 617
HphI GGTGA 3 cut(s) 163, 445, 681
Hpy166II GTNNAC 2 cut(s) 23, 573
Hpy188I TCNGA 2 cut(s) 711, 730
Hpy188III TCNNGA 5 cut(s) 82, 264, 334, 445, 563
Hpy8I GTNNAC 2 cut(s) 23, 573
HpyCH4III ACNGT 1 cut(s) 478
HpyCH4V TGCA 5 cut(s) 286, 408, 548, 583, 683
HpyF3I CTNAG 5 cut(s) 426, 485, 659, 708, 727
Hsp92II CATG 2 cut(s) 202, 625
HspAI GCGC 2 cut(s) 470, 472
Kzo9I GATC 3 cut(s) 168, 321, 330
LguI GCTCTTC 1 cut(s) 189
LmnI GCTCC 1 cut(s) 422
Lsp1109I GCAGC 1 cut(s) 667
LweI GCATC 1 cut(s) 295
MaeI CTAG 2 cut(s) 26, 48
MalI GATC 3 cut(s) 170, 323, 332
MboI GATC 3 cut(s) 168, 321, 330
MboII GAAGA 5 cut(s) 176, 286, 287, 374, 499
MflI RGATCY 2 cut(s) 321, 330
MhlI GDGCHC 2 cut(s) 427, 519
MluCI AATT 2 cut(s) 234, 720
MlyI GAGTC 3 cut(s) 137, 143, 626
MnlI CCTC 4 cut(s) 42, 348, 455, 585
MseI TTAA 2 cut(s) 630, 754
MspR9I CCNGG 2 cut(s) 258, 512
MvaI CCWGG 2 cut(s) 258, 512
MvnI CGCG 1 cut(s) 472
NcoI CCATGG 1 cut(s) 621
NdeI CATATG 1 cut(s) 526
NdeII GATC 3 cut(s) 168, 321, 330
NlaIII CATG 2 cut(s) 202, 625
PauI GCGCGC 1 cut(s) 470
PciSI GCTCTTC 1 cut(s) 189
PfeI GAWTC 1 cut(s) 51
PkrI GCNGC 1 cut(s) 682
PleI GAGTC 3 cut(s) 137, 142, 625
PpsI GAGTC 3 cut(s) 137, 142, 625
Psp124BI GAGCTC 1 cut(s) 427
Psp6I CCWGG 2 cut(s) 256, 510
PspGI CCWGG 2 cut(s) 256, 510
PspPI GGNCC 2 cut(s) 105, 349
PstI CTGCAG 1 cut(s) 550
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 2 cut(s) 321, 330
PteI GCGCGC 1 cut(s) 470
RsaI GTAC 1 cut(s) 698
RsaNI GTAC 1 cut(s) 697
SacI GAGCTC 1 cut(s) 427
SapI GCTCTTC 1 cut(s) 189
SaqAI TTAA 2 cut(s) 630, 754
SatI GCNGC 1 cut(s) 681
Sau3AI GATC 3 cut(s) 168, 321, 330
Sau96I GGNCC 2 cut(s) 105, 349
SchI GAGTC 3 cut(s) 137, 143, 626
ScrFI CCNGG 2 cut(s) 258, 512
SduI GDGCHC 2 cut(s) 427, 519
SetI ASST 8 cut(s) 107, 113, 176, 184, 251, 304, 427, 452
SfaNI GCATC 1 cut(s) 295
SfcI CTRYAG 2 cut(s) 376, 546
SinI GGWCC 1 cut(s) 105
SmlI CTYRAG 1 cut(s) 445
SmoI CTYRAG 1 cut(s) 445
SpeI ACTAGT 1 cut(s) 25
Sse9I AATT 2 cut(s) 234, 720
SspI AATATT 1 cut(s) 675
SspMI CTAG 2 cut(s) 26, 48
SstI GAGCTC 1 cut(s) 427
StyD4I CCNGG 2 cut(s) 256, 510
StyI CCWWGG 3 cut(s) 163, 497, 621
TaaI ACNGT 1 cut(s) 478
TasI AATT 2 cut(s) 234, 720
TfiI GAWTC 1 cut(s) 51
Tru1I TTAA 2 cut(s) 630, 754
Tru9I TTAA 2 cut(s) 630, 754
TscAI CASTG 7 cut(s) 78, 229, 288, 434, 543, 614, 667
TseI GCWGC 1 cut(s) 680
TspDTI ATGAA 5 cut(s) 87, 104, 176, 187, 374
TspRI CASTG 7 cut(s) 78, 229, 288, 434, 543, 614, 667
VpaK11BI GGWCC 1 cut(s) 105
XspI CTAG 2 cut(s) 26, 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.