MD13G1048100.v1.1

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014445
Physical Location & Seq
Reverse (-)
3366934 .. 3368964
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1048100.v1.1.491

Sequence Viewer

Length: 735 bp
ATGGCGACAGTCACACCATCACTAGTTCCACTCCTCTTCTTTGCCAGAATCTCAGGGCTTGCAGTTGCAGCTCTGGTCATTTTCTGGGCTTTCTCCTTCAAGTCCAGCTTCCTTCCTCATTCTTCCCCACAAGAAGACCAAGGCCTCATTTATTCAGCTCTTCATCCTCTGTTCATGGTCATTGGGTTTATTCTCATTAGTGGAGAAGCAATTTTGATCCATAGGTGGTTGCCTGGTTCAAGGAGTTTGAAGAAATCAGTGCATTTGTGCCTTCAAGGCGTGGCTTTGGCTTCCGGGATAGTTGGGATTTGGACTAAATTTCAAGGGAAGAAGGGCATGGTGTCTAATTTCTATAGTTTGCATTCTTGGATGGGGTTGATCTGCATCCTCTTGTTTGGAGCTCAGTGGTTGATGGGGTTCCTGAGCTTCTGGCATCGAGGGGAAGTGCGCTCGGTGAGGCTAAGGGTGTTGCCGTGGCACATCTTCCTTGGACTCTACACGTACGGTCTGGCTGTGGCAACTGCAGAAACGGGGCTTTTGGAGAAGTTGACATTCTTACAAACGAAGAGGAATGTGTCTAAACACTGTACCGAGTCGATGCTTGTGAATAGTTTGGGACTGGGGCTGGCATTGGTCAGCGGCATTGTAATTTTGGCTGCAGTGTCGCCTAAATACCAAGCCCTTCAAAGTAAACTCATTTACTCGTCAGATGGCAAAAAATGCATGTCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

245

Amino Acids

26.83

Weight (kDa)

9.9

Isoelectric Point (pI)

47.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 54 - 185 7.5e-41 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014445)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26100
fragaria_vesca FvH4_4g28220 FvH4_4g28220 FvH4_4g28220 FvH4_4g28220
malus_domestica MD13G1048100.v1.1
prunus_persica Prupe.1G304000_v2.0.a1
pyrus_communis pycom13g04220
rosa_chinensis RchiOBHm_Chr4g0436011
rosa_laevigata RLG00000006542
rosa_roxburghii Rroxscaffold_5G00377160
rosa_rugosa Rorug04G0291200
rosa_samantha Rh4AG345500 Rh4BG354500 Rh4CG369000 Rh4DG348200
rosa_wichuraiana Rw4G030210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 639
AclWI GGATC 1 cut(s) 211
AcsI RAATTY 1 cut(s) 317
AfaI GTAC 2 cut(s) 503, 589
AflIII ACRYGT 1 cut(s) 498
AgsI TTSAA 6 cut(s) 100, 240, 250, 275, 323, 686
AhlI ACTAGT 1 cut(s) 22
AjnI CCWGG 1 cut(s) 232
AluBI AGCT 5 cut(s) 71, 108, 158, 401, 426
AluI AGCT 5 cut(s) 71, 108, 158, 401, 426
Alw21I GWGCWC 1 cut(s) 403
AlwI GGATC 1 cut(s) 211
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 2 cut(s) 68, 656
ApoI RAATTY 1 cut(s) 317
AspLEI GCGC 1 cut(s) 450
AsuC2I CCSGG 1 cut(s) 295
AsuHPI GGTGA 1 cut(s) 466
BanII GRGCYC 1 cut(s) 403
BbsI GAAGAC 2 cut(s) 141, 720
Bbv12I GWGCWC 1 cut(s) 403
BbvI GCAGC 2 cut(s) 80, 643
BccI CCATC 4 cut(s) 25, 364, 406, 704
BceAI ACGGC 1 cut(s) 457
BciT130I CCWGG 1 cut(s) 234
BcnI CCSGG 1 cut(s) 295
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 3 cut(s) 352, 522, 657
BglI GCCNNNNNGGC 1 cut(s) 276
BisI GCNGC 3 cut(s) 69, 640, 657
BlsI GCNGC 3 cut(s) 70, 641, 658
Bme1390I CCNGG 2 cut(s) 234, 295
BmiI GGNNCC 1 cut(s) 419
BmrFI CCNGG 2 cut(s) 234, 295
BmrI ACTGGG 1 cut(s) 629
BmsI GCATC 3 cut(s) 393, 442, 588
BmuI ACTGGG 1 cut(s) 629
BpiI GAAGAC 2 cut(s) 141, 720
Bpu10I CCTNAGC 2 cut(s) 422, 461
BpuMI CCSGG 1 cut(s) 295
BsaAI YACGTR 1 cut(s) 501
BsaBI GATNNNNATC 1 cut(s) 383
BsaJI CCNNGG 3 cut(s) 139, 473, 487
Bse1I ACTGG 1 cut(s) 624
Bse8I GATNNNNATC 1 cut(s) 383
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 3 cut(s) 139, 473, 487
BseGI GGATG 3 cut(s) 163, 375, 384
BseJI GATNNNNATC 1 cut(s) 383
BseMII CTCAG 3 cut(s) 66, 413, 416
BseNI ACTGG 1 cut(s) 624
BseRI GAGGAG 1 cut(s) 23
BseXI GCAGC 2 cut(s) 80, 643
BshFI GGCC 1 cut(s) 144
BsiHKAI GWGCWC 1 cut(s) 403
BsiSI CCGG 1 cut(s) 294
BsiWI CGTACG 1 cut(s) 501
BslFI GGGAC 1 cut(s) 630
BsmFI GGGAC 1 cut(s) 630
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 1 cut(s) 144
Bsp1286I GDGCHC 1 cut(s) 403
Bsp143I GATC 2 cut(s) 216, 378
BspACI CCGC 1 cut(s) 639
BspANI GGCC 1 cut(s) 144
BspCNI CTCAG 3 cut(s) 65, 414, 415
BspLI GGNNCC 1 cut(s) 419
BspMAI CTGCAG 2 cut(s) 526, 661
BspPI GGATC 1 cut(s) 211
BspQI GCTCTTC 1 cut(s) 165
BsrI ACTGG 1 cut(s) 624
BssECI CCNNGG 3 cut(s) 139, 473, 487
BssMI GATC 2 cut(s) 216, 378
BssT1I CCWWGG 2 cut(s) 139, 487
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 3 cut(s) 10, 506, 587
Bst6I CTCTTC 3 cut(s) 41, 165, 560
BstAPI GCANNNNNTGC 1 cut(s) 720
BstBAI YACGTR 1 cut(s) 501
BstC8I GCNNGC 2 cut(s) 60, 627
BstDEI CTNAG 4 cut(s) 52, 402, 422, 461
BstDSI CCRYGG 1 cut(s) 473
BstF5I GGATG 3 cut(s) 163, 375, 384
BstHHI GCGC 1 cut(s) 450
BstKTI GATC 2 cut(s) 219, 381
BstMBI GATC 2 cut(s) 216, 378
BstMWI GCNNNNNNNGC 3 cut(s) 68, 276, 720
BstNI CCWGG 1 cut(s) 234
BstNSI RCATGY 1 cut(s) 727
BstSCI CCNGG 2 cut(s) 232, 293
BstSFI CTRYAG 3 cut(s) 352, 522, 657
BstV1I GCAGC 2 cut(s) 80, 643
BstV2I GAAGAC 2 cut(s) 141, 720
BsuRI GGCC 1 cut(s) 144
BtgI CCRYGG 1 cut(s) 473
BtsCI GGATG 3 cut(s) 163, 375, 384
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 4 cut(s) 264, 410, 583, 666
Cac8I GCNNGC 2 cut(s) 60, 627
CfoI GCGC 1 cut(s) 450
Csp6I GTAC 2 cut(s) 502, 588
CviAII CATG 3 cut(s) 175, 337, 724
CviQI GTAC 2 cut(s) 502, 588
DdeI CTNAG 4 cut(s) 52, 402, 422, 461
DpnI GATC 2 cut(s) 218, 380
DpnII GATC 2 cut(s) 216, 378
Eam1104I CTCTTC 3 cut(s) 41, 165, 560
EarI CTCTTC 3 cut(s) 41, 165, 560
Ecl136II GAGCTC 1 cut(s) 401
Eco130I CCWWGG 2 cut(s) 139, 487
Eco147I AGGCCT 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 403
Eco53kI GAGCTC 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 401
EcoRII CCWGG 1 cut(s) 232
EcoT14I CCWWGG 2 cut(s) 139, 487
EcoT22I ATGCAT 1 cut(s) 725
EcoT38I GRGCYC 1 cut(s) 403
ErhI CCWWGG 2 cut(s) 139, 487
FaeI CATG 3 cut(s) 178, 340, 727
FaiI YATR 5 cut(s) 176, 222, 338, 354, 725
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FaqI GGGAC 1 cut(s) 630
FatI CATG 3 cut(s) 174, 336, 723
Fnu4HI GCNGC 3 cut(s) 69, 640, 657
FokI GGATG 3 cut(s) 150, 371, 382
FriOI GRGCYC 1 cut(s) 403
Fsp4HI GCNGC 3 cut(s) 69, 640, 657
FspBI CTAG 1 cut(s) 23
GlaI GCGC 1 cut(s) 449
GluI GCNGC 3 cut(s) 69, 640, 657
HaeIII GGCC 1 cut(s) 144
HapII CCGG 1 cut(s) 294
HhaI GCGC 1 cut(s) 450
Hin1II CATG 3 cut(s) 178, 340, 727
Hin6I GCGC 1 cut(s) 448
HinP1I GCGC 1 cut(s) 448
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HinfI GANTC 3 cut(s) 48, 492, 593
HpaII CCGG 1 cut(s) 294
HphI GGTGA 1 cut(s) 466
Hpy166II GTNNAC 2 cut(s) 549, 692
Hpy188I TCNGA 1 cut(s) 709
Hpy188III TCNNGA 1 cut(s) 421
Hpy8I GTNNAC 2 cut(s) 549, 692
HpyAV CCTTC 5 cut(s) 106, 122, 281, 325, 692
HpyCH4III ACNGT 3 cut(s) 10, 506, 587
HpyCH4IV ACGT 1 cut(s) 500
HpyCH4V TGCA 8 cut(s) 62, 68, 262, 361, 384, 524, 659, 723
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 276, 720
HpyF3I CTNAG 4 cut(s) 52, 402, 422, 461
HpySE526I ACGT 1 cut(s) 500
Hsp92II CATG 3 cut(s) 178, 340, 727
HspAI GCGC 1 cut(s) 448
Kzo9I GATC 2 cut(s) 216, 378
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 1 cut(s) 398
Lsp1109I GCAGC 2 cut(s) 80, 643
LweI GCATC 3 cut(s) 393, 442, 588
MaeI CTAG 1 cut(s) 23
MaeII ACGT 1 cut(s) 500
MaeIII GTNAC 1 cut(s) 10
MalI GATC 2 cut(s) 218, 380
MboI GATC 2 cut(s) 216, 378
MboII GAAGA 9 cut(s) 28, 114, 146, 152, 262, 340, 475, 577, 720
MhlI GDGCHC 1 cut(s) 403
MluCI AATT 4 cut(s) 210, 317, 346, 648
MlyI GAGTC 2 cut(s) 486, 602
MnlI CCTC 8 cut(s) 44, 126, 155, 177, 398, 431, 450, 561
Mph1103I ATGCAT 1 cut(s) 725
MseI TTAA 1 cut(s) 733
MspA1I CMGCKG 1 cut(s) 639
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 2 cut(s) 234, 295
Mva1269I GAATGC 1 cut(s) 361
MvaI CCWGG 1 cut(s) 234
MwoI GCNNNNNNNGC 3 cut(s) 68, 276, 720
NciI CCSGG 1 cut(s) 295
NdeII GATC 2 cut(s) 216, 378
NlaIII CATG 3 cut(s) 178, 340, 727
NlaIV GGNNCC 1 cut(s) 419
NmuCI GTSAC 1 cut(s) 10
NsiI ATGCAT 1 cut(s) 725
NspI RCATGY 1 cut(s) 727
PceI AGGCCT 1 cut(s) 144
PciSI GCTCTTC 1 cut(s) 165
PctI GAATGC 1 cut(s) 361
PfeI GAWTC 1 cut(s) 48
Pfl23II CGTACG 1 cut(s) 501
PfoI TCCNGGA 1 cut(s) 293
PkrI GCNGC 3 cut(s) 70, 641, 658
PleI GAGTC 2 cut(s) 486, 601
PpsI GAGTC 2 cut(s) 486, 601
Ppu21I YACGTR 1 cut(s) 501
Psp124BI GAGCTC 1 cut(s) 403
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PspLI CGTACG 1 cut(s) 501
PspN4I GGNNCC 1 cut(s) 419
PstI CTGCAG 2 cut(s) 526, 661
RsaI GTAC 2 cut(s) 503, 589
RsaNI GTAC 2 cut(s) 502, 588
SacI GAGCTC 1 cut(s) 403
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 1 cut(s) 733
SatI GCNGC 3 cut(s) 69, 640, 657
Sau3AI GATC 2 cut(s) 216, 378
SchI GAGTC 2 cut(s) 486, 602
ScrFI CCNGG 2 cut(s) 234, 295
SduI GDGCHC 1 cut(s) 403
SetI ASST 7 cut(s) 73, 110, 160, 227, 403, 428, 503
SfaNI GCATC 3 cut(s) 393, 442, 588
SfcI CTRYAG 3 cut(s) 352, 522, 657
SpeI ACTAGT 1 cut(s) 22
Sse9I AATT 4 cut(s) 210, 317, 346, 648
SseBI AGGCCT 1 cut(s) 144
SsiI CCGC 1 cut(s) 639
SspMI CTAG 1 cut(s) 23
SstI GAGCTC 1 cut(s) 403
StuI AGGCCT 1 cut(s) 144
StyD4I CCNGG 2 cut(s) 232, 293
StyI CCWWGG 2 cut(s) 139, 487
TaaI ACNGT 3 cut(s) 10, 506, 587
TaiI ACGT 1 cut(s) 503
TaqI TCGA 2 cut(s) 436, 596
TasI AATT 4 cut(s) 210, 317, 346, 648
TauI GCSGC 1 cut(s) 642
TfiI GAWTC 1 cut(s) 48
Tru1I TTAA 1 cut(s) 733
Tru9I TTAA 1 cut(s) 733
TscAI CASTG 4 cut(s) 264, 410, 590, 666
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 2 cut(s) 68, 656
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 2 cut(s) 152, 163
TspRI CASTG 4 cut(s) 264, 410, 590, 666
XapI RAATTY 1 cut(s) 317
XceI RCATGY 1 cut(s) 727
XspI CTAG 1 cut(s) 23
Zsp2I ATGCAT 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.