Rh4BG354500

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014445
Physical Location & Seq
Reverse (-)
51451690 .. 51453523
1834 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG354500.1

Sequence Viewer

Length: 558 bp
ATGGTCATTGGCTTTATTCTCATCAGTGGAGAAGCAATTTTGATACATAGGTGGTTGCCTGGTTCAAGAAGTTTGAAGAAATCAGTGCATTTGTGTCTTCAAGGTGTGGCTTTGGCTTCTGGGATCTTTGGGATTTGGACCAAGTTTCATGGCCAGAAGGGCATTGTGGCTAATTTCTATAGTCTTCATTCTTGGATGGGTTTGATCTGCATTCTCTCGTTTGGAGCTCAGTGGTTGGTGGGTTTCTTGAGCTTTTGGCATAGAGGGGAAGGGCGCACGGTAAGGCAAAGGGTGTTGCCCTGGCATATCTTCCTTGGGCTCTACACTTATGGTTTGGCAGTGGCTACAGCAGAAACTGGGCTTTTGGAGAAGTTGACATTCTTACAAACACAGAGGAATGTGTCTAAACACAGCCCAGAGTCCATGGTTGTTAATAGTTTAGGGCTTGGGTTGGCCTTCCTCAGTGGCATTGTAATATTGGCTGCAGTATCACCCAAGTACCAACCCATTCAAACTAAACTCATTTACTCAGATACCAAAAAATGTCCATCTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

185

Amino Acids

20.46

Weight (kDa)

9.87

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 1 - 127 3.3e-37 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014445)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26100
fragaria_vesca FvH4_4g28220 FvH4_4g28220 FvH4_4g28220 FvH4_4g28220
malus_domestica MD13G1048100.v1.1
prunus_persica Prupe.1G304000_v2.0.a1
pyrus_communis pycom13g04220
rosa_chinensis RchiOBHm_Chr4g0436011
rosa_laevigata RLG00000006542
rosa_roxburghii Rroxscaffold_5G00377160
rosa_rugosa Rorug04G0291200
rosa_samantha Rh4AG345500 Rh4BG354500 Rh4CG369000 Rh4DG348200
rosa_wichuraiana Rw4G030210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 131
AcoI YGGCCR 1 cut(s) 151
AfaI GTAC 1 cut(s) 500
AgsI TTSAA 4 cut(s) 66, 76, 101, 512
AjnI CCWGG 2 cut(s) 58, 299
AluBI AGCT 2 cut(s) 227, 252
AluI AGCT 2 cut(s) 227, 252
Alw21I GWGCWC 1 cut(s) 229
AlwI GGATC 1 cut(s) 131
AlwNI CAGNNNCTG 1 cut(s) 356
AoxI GGCC 2 cut(s) 151, 453
ApeKI GCWGC 1 cut(s) 482
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 138
AsuHPI GGTGA 1 cut(s) 483
AvaII GGWCC 1 cut(s) 138
BaeI ACNNNNGTAYC 4 cut(s) 35, 68, 482, 515
BalI TGGCCA 1 cut(s) 153
BanII GRGCYC 2 cut(s) 229, 321
BbsI GAAGAC 2 cut(s) 89, 176
Bbv12I GWGCWC 1 cut(s) 229
BbvI GCAGC 1 cut(s) 469
BccI CCATC 2 cut(s) 190, 556
BciT130I CCWGG 2 cut(s) 60, 301
BfmI CTRYAG 3 cut(s) 178, 345, 483
BglI GCCNNNNNGGC 1 cut(s) 159
BisI GCNGC 1 cut(s) 483
BlsI GCNGC 1 cut(s) 484
Bme1390I CCNGG 2 cut(s) 60, 301
Bme18I GGWCC 1 cut(s) 138
BmgT120I GGNCC 1 cut(s) 138
BmrFI CCNGG 2 cut(s) 60, 301
BmrI ACTGGG 1 cut(s) 366
BmuI ACTGGG 1 cut(s) 366
BpiI GAAGAC 2 cut(s) 89, 176
BpuEI CTTGAG 1 cut(s) 268
BsaJI CCNNGG 3 cut(s) 299, 313, 423
Bse1I ACTGG 1 cut(s) 361
BseBI CCWGG 2 cut(s) 60, 301
BseDI CCNNGG 3 cut(s) 299, 313, 423
BseGI GGATG 1 cut(s) 201
BseMII CTCAG 3 cut(s) 242, 475, 543
BseNI ACTGG 1 cut(s) 361
BseXI GCAGC 1 cut(s) 469
BshFI GGCC 2 cut(s) 153, 455
BsiHKAI GWGCWC 1 cut(s) 229
BsmI GAATGC 1 cut(s) 210
BsnI GGCC 2 cut(s) 153, 455
Bsp1286I GDGCHC 2 cut(s) 229, 321
Bsp143I GATC 2 cut(s) 123, 204
Bsp19I CCATGG 1 cut(s) 423
BspANI GGCC 2 cut(s) 153, 455
BspCNI CTCAG 3 cut(s) 241, 474, 542
BspMAI CTGCAG 1 cut(s) 487
BspPI GGATC 1 cut(s) 131
BsrI ACTGG 1 cut(s) 361
BssECI CCNNGG 3 cut(s) 299, 313, 423
BssMI GATC 2 cut(s) 123, 204
BssT1I CCWWGG 2 cut(s) 313, 423
Bst2UI CCWGG 2 cut(s) 60, 301
Bst4CI ACNGT 1 cut(s) 280
BstDEI CTNAG 3 cut(s) 228, 461, 529
BstDSI CCRYGG 1 cut(s) 423
BstF5I GGATG 1 cut(s) 201
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 2 cut(s) 126, 207
BstMBI GATC 2 cut(s) 123, 204
BstMWI GCNNNNNNNGC 1 cut(s) 159
BstNI CCWGG 2 cut(s) 60, 301
BstSCI CCNGG 2 cut(s) 58, 299
BstSFI CTRYAG 3 cut(s) 178, 345, 483
BstV1I GCAGC 1 cut(s) 469
BstV2I GAAGAC 2 cut(s) 89, 176
BstX2I RGATCY 1 cut(s) 123
BstYI RGATCY 1 cut(s) 123
BsuRI GGCC 2 cut(s) 153, 455
BtgI CCRYGG 1 cut(s) 423
BtsCI GGATG 1 cut(s) 201
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 5 cut(s) 31, 90, 236, 345, 469
CaiI CAGNNNCTG 1 cut(s) 356
CfoI GCGC 1 cut(s) 276
Cfr13I GGNCC 1 cut(s) 138
Csp6I GTAC 1 cut(s) 499
CviAII CATG 2 cut(s) 149, 424
CviQI GTAC 1 cut(s) 499
DdeI CTNAG 3 cut(s) 228, 461, 529
DpnI GATC 2 cut(s) 125, 206
DpnII GATC 2 cut(s) 123, 204
EaeI YGGCCR 1 cut(s) 151
Ecl136II GAGCTC 1 cut(s) 227
Eco130I CCWWGG 2 cut(s) 313, 423
Eco24I GRGCYC 2 cut(s) 229, 321
Eco47I GGWCC 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 227
EcoICRI GAGCTC 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 58, 299
EcoT14I CCWWGG 2 cut(s) 313, 423
EcoT38I GRGCYC 2 cut(s) 229, 321
ErhI CCWWGG 2 cut(s) 313, 423
FaeI CATG 2 cut(s) 152, 427
FaiI YATR 7 cut(s) 48, 150, 180, 261, 306, 330, 425
FatI CATG 2 cut(s) 148, 423
Fnu4HI GCNGC 1 cut(s) 483
FokI GGATG 1 cut(s) 208
FriOI GRGCYC 2 cut(s) 229, 321
Fsp4HI GCNGC 1 cut(s) 483
GlaI GCGC 1 cut(s) 275
GluI GCNGC 1 cut(s) 483
HaeIII GGCC 2 cut(s) 153, 455
HhaI GCGC 1 cut(s) 276
Hin1II CATG 2 cut(s) 152, 427
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HinfI GANTC 1 cut(s) 419
HphI GGTGA 1 cut(s) 483
Hpy166II GTNNAC 1 cut(s) 375
Hpy188I TCNGA 1 cut(s) 532
Hpy188III TCNNGA 2 cut(s) 66, 247
Hpy8I GTNNAC 1 cut(s) 375
HpyAV CCTTC 3 cut(s) 151, 263, 466
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4V TGCA 3 cut(s) 88, 210, 485
HpyF10VI GCNNNNNNNGC 1 cut(s) 159
HpyF3I CTNAG 3 cut(s) 228, 461, 529
Hsp92II CATG 2 cut(s) 152, 427
HspAI GCGC 1 cut(s) 274
Kzo9I GATC 2 cut(s) 123, 204
LmnI GCTCC 1 cut(s) 224
LpnPI CCDG 8 cut(s) 45, 72, 105, 167, 286, 313, 342, 429
Lsp1109I GCAGC 1 cut(s) 469
MalI GATC 2 cut(s) 125, 206
MboI GATC 2 cut(s) 123, 204
MboII GAAGA 4 cut(s) 88, 89, 176, 301
MflI RGATCY 1 cut(s) 123
MhlI GDGCHC 2 cut(s) 229, 321
MlsI TGGCCA 1 cut(s) 153
MluCI AATT 2 cut(s) 36, 172
MluNI TGGCCA 1 cut(s) 153
MlyI GAGTC 1 cut(s) 428
MnlI CCTC 3 cut(s) 257, 387, 470
Mox20I TGGCCA 1 cut(s) 153
MscI TGGCCA 1 cut(s) 153
MseI TTAA 1 cut(s) 432
Msp20I TGGCCA 1 cut(s) 153
MspR9I CCNGG 2 cut(s) 60, 301
Mva1269I GAATGC 1 cut(s) 210
MvaI CCWGG 2 cut(s) 60, 301
MwoI GCNNNNNNNGC 1 cut(s) 159
NcoI CCATGG 1 cut(s) 423
NdeII GATC 2 cut(s) 123, 204
NlaIII CATG 2 cut(s) 152, 427
PctI GAATGC 1 cut(s) 210
PkrI GCNGC 1 cut(s) 484
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
Psp124BI GAGCTC 1 cut(s) 229
Psp6I CCWGG 2 cut(s) 58, 299
PspGI CCWGG 2 cut(s) 58, 299
PspPI GGNCC 1 cut(s) 138
PstI CTGCAG 1 cut(s) 487
PstNI CAGNNNCTG 1 cut(s) 356
PsuI RGATCY 1 cut(s) 123
RsaI GTAC 1 cut(s) 500
RsaNI GTAC 1 cut(s) 499
SacI GAGCTC 1 cut(s) 229
SaqAI TTAA 1 cut(s) 432
SatI GCNGC 1 cut(s) 483
Sau3AI GATC 2 cut(s) 123, 204
Sau96I GGNCC 1 cut(s) 138
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 2 cut(s) 60, 301
SduI GDGCHC 2 cut(s) 229, 321
SetI ASST 4 cut(s) 53, 106, 229, 254
SfcI CTRYAG 3 cut(s) 178, 345, 483
SinI GGWCC 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 247
SmoI CTYRAG 1 cut(s) 247
Sse9I AATT 2 cut(s) 36, 172
SspI AATATT 1 cut(s) 477
SstI GAGCTC 1 cut(s) 229
StyD4I CCNGG 2 cut(s) 58, 299
StyI CCWWGG 2 cut(s) 313, 423
TaaI ACNGT 1 cut(s) 280
TasI AATT 2 cut(s) 36, 172
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TscAI CASTG 5 cut(s) 31, 90, 236, 345, 469
TseI GCWGC 1 cut(s) 482
TspDTI ATGAA 2 cut(s) 137, 176
TspRI CASTG 5 cut(s) 31, 90, 236, 345, 469
VpaK11BI GGWCC 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.