FvH4_4g29000

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0021068
Physical Location & Seq
Reverse (-)
29370917 .. 29375086
4170 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29000.t1

Sequence Viewer

Length: 2202 bp
ATGGTGCCTGATCGGAGGGAAGCAGTGCTAGAGCCACCGAATTTAACAGAGCAATTGTCTAGGTTTAAGCTACTCCCTTCTTCTGTGGAGGTTGTGACTCCTTCAGTTGTGACTCCCCCGTCAAAGATAGAGGATGTAAGGCCTTGGAAGTTAAGGACTCATAACTTGGGTCTGAGGAAAGCTAGAAAGAGAAGCTTTGAGGCAAGGAAAGTTTTGGCTCTATATCAAGATCATGTGTCTGATCCTGGTCTTGGGTCTGCTTCTGACTCTTGTTCTGATTCTAGTATAATTGGAGAAGGACCGAATCTTGATTCCTCTAGATCTTTGTCTAGGGAGGTCTACCACAGCTTTGCATACCGTGCAGTAGTGTATATCTTTATAACATACCTCATCGATAGTTGGACGTACGAGCATTTTCAGATTTCTGTTGGAATCACATATGCGTCAGATGCCGTAATAAGCATGATGATAATACTCCTTGCTCACATCTCAGACAAGTACTGCATGGATGACCATATTATTATGATCATCTCCACGAATGTAGCCTACATACTTGGATTGATTCTATTAAGCTCTTCGAAAGCGGTGAAGAACAAGAATATTGCAATTGGGGAAATCTATGGATCATTGTTGCTACTATGCGTTGGTGAAGCAGGACGAAGTGCTACACTACATGAATTTTTTGACAACCAATATTCAAGCGAACAAAAACCACAAGAGCCAGATGATGAAAAGTATACGACTCGTCGAGATGCTTTCTGGAAAGTTGCCTGGGTTTTAGGTGCGGTCTTGACCTTCATACCTGGAATTTCCGATGGGGCGAATGCCTCCATAGTTTCAGCAGGTGCCATTGGATCCTCTTGTCTTGTGTTCTGGTTTGGTAGGTCCAAATACTTCATAAATAGTCGAAATTCCAAAGAAACCAAAACAGATCAGAGAGGTAGTCAAATATGCACAGACGATATAAGTATAACCCCACTACCAGCATCAGAAGGGGATCAAAGTGGTCAAGTTTGCATGACTAGTACAGTAGAACAAGAAACAGATCAAAGAAATCAGAATAGCACAGACGACGAAGAAGTGAATGATACTCCTTGTCTAGAAGAAGAAATTTGGGAAACTGGTACAAAAGGGAGCCTCAGTACAGTAGAAGAAGCAGTTGTGGATCCAGAAGCACAAGAAGTGGAACTTTCCATAACCTTGTCTCAGAAGAACGAAATAGGAAGACTAGGGTCTTCCTCAATCAGAAAAACGACCCCCTGGCTAGCAGAAGATTCACAACATATAGGGAAAATTACAGAAGAAGCAGAAAGTGGGGAGCTTAGCGAAGTCGGGGGAACGGAGAATTCCAACAATCTATCAGAAGAATTACAAAGAGGTGAAATTACAGTAGGAGTTGGAGAGCTTAAAGTGAGATCTAAACCAGAACAACCTGGGAGGGGTGAGTTGTGGACAATCACAAAAGTGAAGGAGAAAATACCTATTTTCAGAAAAATGATGCTTATGTGGGCATCCTTCATCATATACAGCATGGTCGATGCAACGGGTAACACATTCTTCTATGGACAAATGAGTAACTTGGATACTTCCACCGAAAGTTTTGCTCTTTATATCTCATCACTAAGCTCCTTTTCAAAATACATAATCTCATTCTCTTATAAAATAGTTGTAACTCCGAAGAAGTGGAGACATGCGACCTTGGTTAGAATTGGTTGTGCAATGGTATGTTCAGTACTGTGTTCCTCTGCTGCCTATTTGGTTGAAACCCATAGGTTGAAATTGGTTAAAAGAGCAGGACTTGATGATGATAGTTACACGACTATTTCCATGGACATCTCTTGGTTAATTCCACAGTTTGTCCTGCATGGGATCATGAGAGGTCTAGCAGAAGAAGGATTGGATGATATTTTCAATGAATTAATCACTGACGGTGATGAACTGATGATTCGGTATAGTTGCCACATCACTGATTTCATCCTCGGTATTACGAAGTCAATCAGCCTTCTAACTATCTTAGTTCCTATTTTGCTGGGTTGCAATTGGTTCGGTGACACCATAAACATGAGTCGCGTAGATAAGTATTACTTGGTGTTAATAATCGCCAGTAGTGTTAACCTTTGCTATTACTTCTATGTCATCTACTCTTATGTCAGAGACAAATCACAAGAGGCTGACGCAGAAGAAAATGAACAAAGCGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

734

Amino Acids

81.75

Weight (kDa)

4.95

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 488 - 625 1.5e-06 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0021068)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g29000
rosa_chinensis RchiOBHm_Chr4g0423201
rosa_multiflora Rmu_sc0007269.1_g000012
rosa_roxburghii Rroxscaffold_5G00365350
rosa_samantha Rh4CG258900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 380, 1659
AarI CACCTGC 1 cut(s) 833
AasI GACNNNNNNGTC 1 cut(s) 118
Acc36I ACCTGC 1 cut(s) 833
AccB1I GGYRCC 2 cut(s) 4, 845
AccI GTMKAC 2 cut(s) 339, 737
AccII CGCG 1 cut(s) 2070
AciI CCGC 2 cut(s) 584, 785
AclWI GGATC 8 cut(s) 236, 631, 849, 862, 1005, 1160, 1173, 1877
AcsI RAATTY 6 cut(s) 40, 677, 807, 910, 1110, 1345
AcuI CTGAAG 1 cut(s) 87
AfaI GTAC 6 cut(s) 407, 500, 1027, 1126, 1144, 1734
AfiI CCNNNNNNNGG 2 cut(s) 251, 1439
AgsI TTSAA 5 cut(s) 699, 1635, 1763, 1777, 1912
AhlI ACTAGT 1 cut(s) 1022
AjnI CCWGG 5 cut(s) 244, 770, 802, 1259, 1432
AleI CACNNNNGTG 1 cut(s) 1463
AluBI AGCT 8 cut(s) 70, 182, 195, 348, 573, 1321, 1405, 1626
AluI AGCT 8 cut(s) 70, 182, 195, 348, 573, 1321, 1405, 1626
Alw26I GTCTC 3 cut(s) 1209, 1681, 2148
AlwI GGATC 8 cut(s) 236, 631, 849, 862, 1005, 1160, 1173, 1877
AoxI GGCC 1 cut(s) 140
ApeKI GCWGC 1 cut(s) 1748
ApoI RAATTY 6 cut(s) 40, 677, 807, 910, 1110, 1345
AseI ATTAAT 1 cut(s) 1919
AspLEI GCGC 1 cut(s) 2198
AspS9I GGNCC 2 cut(s) 299, 885
AsuHPI GGTGA 6 cut(s) 598, 659, 1391, 1454, 1943, 2060
AsuII TTCGAA 1 cut(s) 578
AsuNHI GCTAGC 1 cut(s) 1264
AvaII GGWCC 2 cut(s) 299, 885
BamHI GGATCC 2 cut(s) 854, 1165
BanI GGYRCC 2 cut(s) 4, 845
BbsI GAAGAC 2 cut(s) 1227, 1231
BbvI GCAGC 1 cut(s) 1735
BccI CCATC 1 cut(s) 809
BceAI ACGGC 1 cut(s) 437
BcgI CGANNNNNNTGC 4 cut(s) 1583, 1617, 2026, 2060
BciT130I CCWGG 5 cut(s) 246, 772, 804, 1261, 1434
BciVI GTATCC 1 cut(s) 1576
BclI TGATCA 1 cut(s) 525
BcoDI GTCTC 3 cut(s) 1209, 1681, 2148
BcuI ACTAGT 1 cut(s) 1022
BfuAI ACCTGC 1 cut(s) 833
BfuI GTATCC 1 cut(s) 1576
BglII AGATCT 2 cut(s) 320, 1415
BisI GCNGC 1 cut(s) 1749
BlpI GCTNAGC 1 cut(s) 1322
BlsI GCNGC 1 cut(s) 1750
BmcAI AGTACT 2 cut(s) 500, 1734
Bme1390I CCNGG 5 cut(s) 246, 772, 804, 1261, 1434
Bme18I GGWCC 2 cut(s) 299, 885
BmgT120I GGNCC 2 cut(s) 299, 885
BmiI GGNNCC 5 cut(s) 6, 847, 856, 1136, 1167
BmrFI CCNGG 5 cut(s) 246, 772, 804, 1261, 1434
BmsI GCATC 6 cut(s) 439, 742, 995, 1488, 1520, 1528
BmtI GCTAGC 1 cut(s) 1268
BoxI GACNNNNGTC 1 cut(s) 1231
BpiI GAAGAC 2 cut(s) 1227, 1231
Bpu1102I GCTNAGC 1 cut(s) 1322
Bpu14I TTCGAA 1 cut(s) 578
Bsa29I ATCGAT 1 cut(s) 393
BsaJI CCNNGG 7 cut(s) 143, 771, 1259, 1433, 1698, 1827, 1978
BsaXI ACNNNNNCTCC 2 cut(s) 1126, 1156
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 1439
Bse1I ACTGG 2 cut(s) 1126, 2103
Bse3DI GCAATG 1 cut(s) 1725
BseBI CCWGG 5 cut(s) 246, 772, 804, 1261, 1434
BseCI ATCGAT 1 cut(s) 393
BseDI CCNNGG 7 cut(s) 143, 771, 1259, 1433, 1698, 1827, 1978
BseGI GGATG 5 cut(s) 139, 514, 1511, 1906, 1974
BseLI CCNNNNNNNGG 2 cut(s) 251, 1439
BseMI GCAATG 1 cut(s) 1725
BseMII CTCAG 4 cut(s) 164, 504, 1153, 1220
BseNI ACTGG 2 cut(s) 1126, 2103
BseXI GCAGC 1 cut(s) 1735
BseYI CCCAGC 1 cut(s) 2029
BsgI GTGCAG 1 cut(s) 381
Bsh1236I CGCG 1 cut(s) 2070
BshFI GGCC 1 cut(s) 142
BshNI GGYRCC 2 cut(s) 4, 845
BshVI ATCGAT 1 cut(s) 393
BsiWI CGTACG 1 cut(s) 405
BslI CCNNNNNNNGG 2 cut(s) 251, 1439
BsmAI GTCTC 3 cut(s) 1209, 1681, 2148
BsmI GAATGC 1 cut(s) 829
BsnI GGCC 1 cut(s) 142
Bsp119I TTCGAA 1 cut(s) 578
Bsp1720I GCTNAGC 1 cut(s) 1322
Bsp19I CCATGG 1 cut(s) 1827
BspACI CCGC 2 cut(s) 584, 785
BspANI GGCC 1 cut(s) 142
BspCNI CTCAG 4 cut(s) 165, 503, 1152, 1219
BspDI ATCGAT 1 cut(s) 393
BspFNI CGCG 1 cut(s) 2070
BspHI TCATGA 1 cut(s) 1872
BspLI GGNNCC 5 cut(s) 6, 847, 856, 1136, 1167
BspMI ACCTGC 1 cut(s) 833
BspOI GCTAGC 1 cut(s) 1268
BspPI GGATC 8 cut(s) 236, 631, 849, 862, 1005, 1160, 1173, 1877
BspQI GCTCTTC 1 cut(s) 580
BspT104I TTCGAA 1 cut(s) 578
BspT107I GGYRCC 2 cut(s) 4, 845
BsrDI GCAATG 1 cut(s) 1725
BsrI ACTGG 2 cut(s) 1126, 2103
BssECI CCNNGG 7 cut(s) 143, 771, 1259, 1433, 1698, 1827, 1978
BssNAI GTATAC 1 cut(s) 738
BssT1I CCWWGG 3 cut(s) 143, 1698, 1827
Bst1107I GTATAC 1 cut(s) 738
Bst2UI CCWGG 5 cut(s) 246, 772, 804, 1261, 1434
Bst4CI ACNGT 7 cut(s) 359, 1030, 1147, 1390, 1737, 1854, 1931
Bst6I CTCTTC 1 cut(s) 580
BstAPI GCANNNNNTGC 1 cut(s) 359
BstBI TTCGAA 1 cut(s) 578
BstC8I GCNNGC 1 cut(s) 1266
BstDEI CTNAG 7 cut(s) 173, 490, 1139, 1206, 1322, 1622, 2014
BstDSI CCRYGG 1 cut(s) 1827
BstF5I GGATG 5 cut(s) 139, 514, 1511, 1906, 1974
BstFNI CGCG 1 cut(s) 2070
BstHHI GCGC 1 cut(s) 2198
BstMAI GTCTC 3 cut(s) 1209, 1681, 2148
BstMWI GCNNNNNNNGC 2 cut(s) 359, 449
BstNI CCWGG 5 cut(s) 246, 772, 804, 1261, 1434
BstNSI RCATGY 1 cut(s) 1694
BstPAI GACNNNNGTC 1 cut(s) 1231
BstSCI CCNGG 5 cut(s) 244, 770, 802, 1259, 1432
BstUI CGCG 1 cut(s) 2070
BstV1I GCAGC 1 cut(s) 1735
BstV2I GAAGAC 2 cut(s) 1227, 1231
BstX2I RGATCY 4 cut(s) 320, 854, 1165, 1415
BstYI RGATCY 4 cut(s) 320, 854, 1165, 1415
BstZ17I GTATAC 1 cut(s) 738
Bsu15I ATCGAT 1 cut(s) 393
BsuI GTATCC 1 cut(s) 1576
BsuRI GGCC 1 cut(s) 142
BsuTUI ATCGAT 1 cut(s) 393
BtgI CCRYGG 1 cut(s) 1827
BtsCI GGATG 5 cut(s) 139, 514, 1511, 1906, 1974
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 3 cut(s) 30, 1923, 1965
BveI ACCTGC 1 cut(s) 833
Cac8I GCNNGC 1 cut(s) 1266
CciI TCATGA 1 cut(s) 1872
CfoI GCGC 1 cut(s) 2198
Cfr13I GGNCC 2 cut(s) 299, 885
ClaI ATCGAT 1 cut(s) 393
CseI GACGC 2 cut(s) 432, 2183
Csp6I GTAC 6 cut(s) 406, 499, 1026, 1125, 1143, 1733
CviQI GTAC 6 cut(s) 406, 499, 1026, 1125, 1143, 1733
DdeI CTNAG 7 cut(s) 173, 490, 1139, 1206, 1322, 1622, 2014
DrdI GACNNNNNNGTC 1 cut(s) 118
DseDI GACNNNNNNGTC 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 580
EarI CTCTTC 1 cut(s) 580
Eco130I CCWWGG 3 cut(s) 143, 1698, 1827
Eco147I AGGCCT 1 cut(s) 142
Eco47I GGWCC 2 cut(s) 299, 885
Eco57I CTGAAG 1 cut(s) 87
EcoRI GAATTC 1 cut(s) 1345
EcoRII CCWGG 5 cut(s) 244, 770, 802, 1259, 1432
EcoT14I CCWWGG 3 cut(s) 143, 1698, 1827
ErhI CCWWGG 3 cut(s) 143, 1698, 1827
FalI AAGNNNNNCTT 6 cut(s) 179, 211, 1173, 1205, 2069, 2101
FauNDI CATATG 1 cut(s) 439
FbaI TGATCA 1 cut(s) 525
FblI GTMKAC 2 cut(s) 339, 737
Fnu4HI GCNGC 1 cut(s) 1749
FokI GGATG 5 cut(s) 146, 521, 1498, 1913, 1961
Fsp4HI GCNGC 1 cut(s) 1749
GlaI GCGC 1 cut(s) 2197
GluI GCNGC 1 cut(s) 1749
GsaI CCCAGC 1 cut(s) 2033
HaeIII GGCC 1 cut(s) 142
HgaI GACGC 2 cut(s) 432, 2183
HhaI GCGC 1 cut(s) 2198
Hin6I GCGC 1 cut(s) 2196
HinP1I GCGC 1 cut(s) 2196
HincII GTYRAC 1 cut(s) 2113
HindII GTYRAC 1 cut(s) 2113
HindIII AAGCTT 1 cut(s) 193
HpaI GTTAAC 1 cut(s) 2113
HphI GGTGA 6 cut(s) 598, 659, 1391, 1454, 1943, 2060
Hpy166II GTNNAC 4 cut(s) 340, 738, 1452, 2113
Hpy188III TCNNGA 9 cut(s) 227, 308, 318, 749, 760, 790, 1100, 1169, 1873
Hpy8I GTNNAC 4 cut(s) 340, 738, 1452, 2113
Hpy99I CGWCG 2 cut(s) 750, 1076
HpyAV CCTTC 9 cut(s) 87, 111, 290, 805, 986, 1462, 1525, 1886, 2012
HpyCH4III ACNGT 7 cut(s) 359, 1030, 1147, 1390, 1737, 1854, 1931
HpyCH4IV ACGT 1 cut(s) 404
HpyF10VI GCNNNNNNNGC 2 cut(s) 359, 449
HpyF3I CTNAG 7 cut(s) 173, 490, 1139, 1206, 1322, 1622, 2014
HpySE526I ACGT 1 cut(s) 404
HspAI GCGC 1 cut(s) 2196
Ksp22I TGATCA 1 cut(s) 525
KspAI GTTAAC 1 cut(s) 2113
LguI GCTCTTC 1 cut(s) 580
LmnI GCTCC 3 cut(s) 1134, 1318, 1631
Lsp1109I GCAGC 1 cut(s) 1735
LweI GCATC 6 cut(s) 439, 742, 995, 1488, 1520, 1528
MaeII ACGT 1 cut(s) 404
MaeIII GTNAC 7 cut(s) 94, 109, 1547, 1574, 1669, 1811, 2048
MfeI CAATTG 3 cut(s) 53, 606, 2038
MflI RGATCY 4 cut(s) 320, 854, 1165, 1415
MlyI GAGTC 6 cut(s) 91, 106, 151, 260, 736, 2074
MmeI TCCRAC 4 cut(s) 380, 409, 1374, 1378
MslI CAYNNNNRTG 2 cut(s) 1463, 2060
MspR9I CCNGG 5 cut(s) 246, 772, 804, 1261, 1434
MunI CAATTG 3 cut(s) 53, 606, 2038
Mva1269I GAATGC 1 cut(s) 829
MvaI CCWGG 5 cut(s) 246, 772, 804, 1261, 1434
MvnI CGCG 1 cut(s) 2070
MwoI GCNNNNNNNGC 2 cut(s) 359, 449
NcoI CCATGG 1 cut(s) 1827
NdeI CATATG 1 cut(s) 439
NheI GCTAGC 1 cut(s) 1264
NlaIV GGNNCC 5 cut(s) 6, 847, 856, 1136, 1167
NmuCI GTSAC 3 cut(s) 94, 109, 2048
NspI RCATGY 1 cut(s) 1694
NspV TTCGAA 1 cut(s) 578
OliI CACNNNNGTG 1 cut(s) 1463
PagI TCATGA 1 cut(s) 1872
PaqCI CACCTGC 1 cut(s) 833
PceI AGGCCT 1 cut(s) 142
PciSI GCTCTTC 1 cut(s) 580
PctI GAATGC 1 cut(s) 829
PfeI GAWTC 7 cut(s) 278, 304, 311, 432, 562, 1274, 1945
Pfl23II CGTACG 1 cut(s) 405
PkrI GCNGC 1 cut(s) 1750
PleI GAGTC 6 cut(s) 91, 106, 151, 260, 736, 2073
PpsI GAGTC 6 cut(s) 91, 106, 151, 260, 736, 2073
PshAI GACNNNNGTC 1 cut(s) 1231
PshBI ATTAAT 1 cut(s) 1919
PsiI TTATAA 2 cut(s) 380, 1659
Psp6I CCWGG 5 cut(s) 244, 770, 802, 1259, 1432
PspFI CCCAGC 1 cut(s) 2029
PspGI CCWGG 5 cut(s) 244, 770, 802, 1259, 1432
PspLI CGTACG 1 cut(s) 405
PspN4I GGNNCC 5 cut(s) 6, 847, 856, 1136, 1167
PspPI GGNCC 2 cut(s) 299, 885
PsuI RGATCY 4 cut(s) 320, 854, 1165, 1415
RsaI GTAC 6 cut(s) 407, 500, 1027, 1126, 1144, 1734
RsaNI GTAC 6 cut(s) 406, 499, 1026, 1125, 1143, 1733
RseI CAYNNNNRTG 2 cut(s) 1463, 2060
SapI GCTCTTC 1 cut(s) 580
SatI GCNGC 1 cut(s) 1749
Sau96I GGNCC 2 cut(s) 299, 885
ScaI AGTACT 2 cut(s) 500, 1734
SchI GAGTC 6 cut(s) 91, 106, 151, 260, 736, 2074
ScrFI CCNGG 5 cut(s) 246, 772, 804, 1261, 1434
SfaNI GCATC 6 cut(s) 439, 742, 995, 1488, 1520, 1528
SfuI TTCGAA 1 cut(s) 578
SinI GGWCC 2 cut(s) 299, 885
SmiMI CAYNNNNRTG 2 cut(s) 1463, 2060
SpeI ACTAGT 1 cut(s) 1022
SseBI AGGCCT 1 cut(s) 142
SsiI CCGC 2 cut(s) 584, 785
SspI AATATT 2 cut(s) 601, 695
StuI AGGCCT 1 cut(s) 142
StyD4I CCNGG 5 cut(s) 244, 770, 802, 1259, 1432
StyI CCWWGG 3 cut(s) 143, 1698, 1827
TaaI ACNGT 7 cut(s) 359, 1030, 1147, 1390, 1737, 1854, 1931
TaiI ACGT 1 cut(s) 407
TaqI TCGA 5 cut(s) 393, 578, 748, 907, 1536
TaqII GACCGA 1 cut(s) 316
TatI WGTACW 4 cut(s) 498, 1025, 1142, 1732
TfiI GAWTC 7 cut(s) 278, 304, 311, 432, 562, 1274, 1945
TscAI CASTG 3 cut(s) 30, 1930, 1972
TseFI GTSAC 3 cut(s) 94, 109, 2048
TseI GCWGC 1 cut(s) 1748
Tsp45I GTSAC 3 cut(s) 94, 109, 2048
TspDTI ATGAA 9 cut(s) 690, 744, 787, 886, 1507, 1929, 1950, 1963, 2202
TspGWI ACGGA 1 cut(s) 1355
TspRI CASTG 3 cut(s) 30, 1930, 1972
VpaK11BI GGWCC 2 cut(s) 299, 885
VspI ATTAAT 1 cut(s) 1919
XapI RAATTY 6 cut(s) 40, 677, 807, 910, 1110, 1345
XbaI TCTAGA 2 cut(s) 317, 1099
XceI RCATGY 1 cut(s) 1694
XmiI GTMKAC 2 cut(s) 339, 737
ZrmI AGTACT 2 cut(s) 500, 1734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.