Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0010093

Physical Location & Seq

Genomic Coordinates Reverse (-)

30049900 .. 30052507

Gene Structure 2608 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g30400.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1884 bp

ATGGCCGTTGCTCCGCCGCAGCTCGTTTTCTTCTTCGTTCTTATAATCGGGTTCTGTTTCGACCCGACCCATCAGAAACCCTCGGTGTCGTTAAACACGACGAGGCTGTCGAAATCCGGCGACTCGGTTCTGATCAAATGGTCCGGGGTCGACTCGCCGTCGAAGTTGGACTGGCTCGGAATCTACTCGCCGCCGTCGTCACGCAACCACCACTTCCTCGGGTACAAGTTCCTCTCCTCCAGCCCGACATGGCAATCCGGGTCGGGCTCCATTTCGCTTCCCTTGATCAACCTCCGATCCAACTACTCCTTCCGGATCTTCCGCTGGTCCGAGTCCGAGGTCAATCCCGACAAACGCGACCACGACGACAACCCCCTCCCGGGGATCAAACACCTCCTCGCCACGTCGCCGGAGCTCGCTTTTGAATCGGGTCGGGTCCCGGACCAGATCCACTTATCCTACACCGACCGCCTCGACGAGATGCGCGTCATGTTCGTGACCCCGGACCGCGATCAACGCGCCGTGAAATACGGCGCGAGAAAGGACGGACTGGATGACGTGGCGGCCGCACGTGTGAGCCGGTACGAGCTGCAGCACATGTGCGACTGGCCGGCGAATCACAGCGTCGGGTGGAGAGACCCTGGGTTTGTTCACGATGGCGTCATGACCAATCTCAAAAGCGGTGTCAGATATTATTACAAGGTTGGGAGTGATGATGGAGGTTGGAGTGAAACGCACAGCTTCGTATCGAGAAATGGAGATTCCGACGAGGCGGTAGCTTTCATGTTTGGTGACATGGGCACTACGACGCCGTACGCGACGTTTTTTCATATGCAAGACGAAAGTGTGGCCACCATCAAGTGGATTCTGCGTGACATTGAAGCTCTCGGCGACAAGCCGGCTTTCGTGTCCCACATTGGGGATATAAGCTACGCGAGAGGCTATTCGTGGCTGTGGGATCATTTCTTCAATCAGATTGAGCCGGTTGCGACGAGGCTGCCGTATCATGTTTGCATTGGGAATCATGAGTATGACTGGCCGTTGCAGCCGTGGAAGCCGGAGTGGGCTAGTTCGATTTATGGGAAAGATGGTGGTGGAGAGTGTGGGGTGCCGTATAGTGTGAGGTTCAACATGCCTGGGAACTCGTCTGAGTCAACTGGGACTAGTGCGCCCGCGACTAGGAACCTTTACTATTCGTTTGACATGGGGTCGGTGCATTTTGTGTACATTTCGACCGAGACTAATTTTGTGACGGGAAGCAAGCAGCATGAGTTTATAAAGAGGGATTTGGAGAGTGTTAATCGGACGAAGACTCCTTTCGTGGTGGTTAGCGGACACAGGCCGATGTACACTACAAGCAATGAGGGAAGGGATGCGGAGATGAGGAGGCAGATGATGGAGCATTTGGAGCCTTTGTTTGTGAACAATAATGTGACTCTTGCGTTATGGGGACATGTTCATAGATACGAGAGGTTTTGTCCGTTGAGTAATTTTACTTGTGGGAGTAGAGGGCCTGTTCATGTTGTGATTGGGATGGCAGGACAGGACTGGCAGCCGATATGGCAACCTAGAGCGGATCACCCAACTGTCCCCATATTCCCACAGCCACTGAGGTCTATGTACCGCGGTGGTGAGTTTGGGTATACTAGGTTGGTTGCTTCGAAAGAGAAGCTTACACTTTCTTATGTTGGAAACCATGATGGACAGGTGCATGATTCGGTGACGATTCTGGCTTCGGGAGAGGTTCTGGGGGGTGAAGCAGGTGGTGCTGGTGTCAATGCTCTTGGTAATGGGATCAAAGCTGCTGTGAATGATAGTGATGGTTATGGTGTTGCTGGATTTGGGGGAAAAAGGCCGTCCCAAAAAATAGTTGGATTCCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0005048 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005768 GO:0005794 GO:0005802 GO:0006109 GO:0006605 GO:0006626 GO:0006793 GO:0006796 GO:0006810 GO:0006839 GO:0006886 GO:0006996 GO:0007005 GO:0007006 GO:0008104 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0017038 GO:0019222 GO:0030943 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0031984 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0042277 GO:0042578 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044429 GO:0044431 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045040 GO:0045184 GO:0046907 GO:0050789 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061024 GO:0065007 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0072594 GO:0072655 GO:0072657 GO:0080090 GO:0090150 GO:0090151 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

628

Amino Acids

70.03

Weight (kDa)

6.34

Isoelectric Point (pI)

37.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pur_ac_phosph_N	PF16656	147 - 249	2.4e-19	Purple acid Phosphatase, N-terminal domain
Metallophos	PF00149	279 - 489	1.3e-12	Calcineurin-like phosphoesterase
Metallophos_C	PF14008	504 - 573	1.4e-14	Iron/zinc purple acid phosphatase-like protein C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010093)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13900 AT2G03450
fragaria_vesca	FvH4_4g30400 FvH4_4g30400
malus_domestica	MD13G1064900.v1.1
prunus_persica	Prupe.1G283200_v2.0.a1 Prupe.1G283200_v2.0.a1
pyrus_communis	pycom13g05730
rosa_chinensis	RchiOBHm_Chr4g0438691
rosa_laevigata	RLG00000006337
rosa_multiflora	Rmu_co8427227.1_g000001 Rmu_sc0000738.1_g000044 Rmu_sc0012315.1_g000001
rosa_roxburghii	Rroxscaffold_5G00379730
rosa_rugosa	Rorug04G0312700
rosa_samantha	Rh4AG363200 Rh4BG374600 Rh4CG388800 Rh4DG369100
rosa_wichuraiana	Rw0G007170 Rw0G016710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	44, 1277
AarI	CACCTGC	1 cut(s)	1751
AasI	GACNNNNNNGTC	1 cut(s)	106
Acc36I	ACCTGC	1 cut(s)	1751
AccB1I	GGYRCC	1 cut(s)	1108
AccB7I	CCANNNNNTGG	1 cut(s)	861
AccBSI	CCGCTC	1 cut(s)	1574
AccI	GTMKAC	2 cut(s)	150, 1643
AccII	CGCG	9 cut(s)	357, 486, 510, 519, 536, 818, 935, 1175, 1626
AccIII	TCCGGA	1 cut(s)	312
AclWI	GGATC	7 cut(s)	291, 323, 392, 442, 966, 1584, 1802
AcoI	YGGCCR	5 cut(s)	3, 564, 608, 849, 1037
AcvI	CACGTG	1 cut(s)	572
AcyI	GRCGYC	2 cut(s)	660, 809
AfaI	GTAC	6 cut(s)	224, 584, 815, 1226, 1349, 1622
AfiI	CCNNNNNNNGG	7 cut(s)	379, 380, 381, 861, 918, 919, 1179
AflIII	ACRYGT	3 cut(s)	571, 597, 1453
AgsI	TTSAA	4 cut(s)	425, 881, 970, 1129
AhdI	GACNNNNNGTC	2 cut(s)	157, 1207
AhlI	ACTAGT	1 cut(s)	1163
AjiI	CACGTC	2 cut(s)	405, 559
AjnI	CCWGG	2 cut(s)	640, 1135
AjuI	GAANNNNNNNTTGG	2 cut(s)	293, 325
AluBI	AGCT	9 cut(s)	22, 415, 589, 741, 779, 884, 930, 1672, 1802
AluI	AGCT	9 cut(s)	22, 415, 589, 741, 779, 884, 930, 1672, 1802
Alw21I	GWGCWC	1 cut(s)	417
Alw26I	GTCTC	2 cut(s)	630, 1232
AlwI	GGATC	7 cut(s)	291, 323, 392, 442, 966, 1584, 1802
AlwNI	CAGNNNCTG	1 cut(s)	1609
Ama87I	CYCGRG	2 cut(s)	218, 379
Aor13HI	TCCGGA	1 cut(s)	312
AoxI	GGCC	8 cut(s)	3, 564, 608, 849, 1037, 1340, 1511, 1853
ApeKI	GCWGC	8 cut(s)	19, 589, 592, 997, 1045, 1264, 1552, 1802
ArsI	GACNNNNNNTTYG	4 cut(s)	629, 661, 669, 701
AspLEI	GCGC	4 cut(s)	486, 521, 536, 1171
AspS9I	GGNCC	6 cut(s)	141, 327, 436, 442, 505, 1511
AsuC2I	CCSGG	6 cut(s)	145, 259, 380, 381, 440, 503
AsuHPI	GGTGA	5 cut(s)	803, 1571, 1643, 1732, 1766
AsuII	TTCGAA	1 cut(s)	1661
AvaI	CYCGRG	2 cut(s)	218, 379
AvaII	GGWCC	5 cut(s)	141, 327, 436, 442, 505
BaeGI	GKGCMC	1 cut(s)	803
BalI	TGGCCA	1 cut(s)	851
BanI	GGYRCC	1 cut(s)	1108
BanII	GRGCYC	2 cut(s)	269, 417
BbrPI	CACGTG	1 cut(s)	572
BbsI	GAAGAC	1 cut(s)	1316
Bbv12I	GWGCWC	1 cut(s)	417
BbvI	GCAGC	8 cut(s)	31, 576, 604, 984, 1057, 1276, 1564, 1789
BccI	CCATC	9 cut(s)	78, 650, 710, 863, 1082, 1390, 1528, 1694, 1814
BcgI	CGANNNNNNTGC	2 cut(s)	979, 1013
BciT130I	CCWGG	2 cut(s)	642, 1137
BclI	TGATCA	2 cut(s)	132, 285
BcnI	CCSGG	6 cut(s)	145, 259, 380, 381, 440, 503
BcoDI	GTCTC	2 cut(s)	630, 1232
BcuI	ACTAGT	1 cut(s)	1163
BfaI	CTAG	5 cut(s)	1068, 1164, 1179, 1569, 1647
BfmI	CTRYAG	1 cut(s)	590
BfuAI	ACCTGC	1 cut(s)	1751
BglI	GCCNNNNNGGC	1 cut(s)	1561
Bme1390I	CCNGG	8 cut(s)	145, 259, 380, 381, 440, 503, 642, 1137
Bme18I	GGWCC	5 cut(s)	141, 327, 436, 442, 505
BmeRI	GACNNNNNGTC	2 cut(s)	157, 1207
BmeT110I	CYCGRG	2 cut(s)	218, 379
BmgBI	CACGTC	2 cut(s)	405, 559
BmgT120I	GGNCC	6 cut(s)	141, 327, 436, 442, 505, 1511
BmiI	GGNNCC	6 cut(s)	268, 437, 438, 1110, 1184, 1410
BmrFI	CCNGG	8 cut(s)	145, 259, 380, 381, 440, 503, 642, 1137
BmrI	ACTGGG	1 cut(s)	1167
BmsI	GCATC	2 cut(s)	471, 1363
BmuI	ACTGGG	1 cut(s)	1167
BpiI	GAAGAC	1 cut(s)	1316
BpmI	CTGGAG	1 cut(s)	223
Bpu14I	TTCGAA	1 cut(s)	1661
BpuMI	CCSGG	6 cut(s)	145, 259, 380, 381, 440, 503
BsaAI	YACGTR	1 cut(s)	572
BsaHI	GRCGYC	2 cut(s)	660, 809
BsaI	GGTCTC	1 cut(s)	630
BsaWI	WCCGGW	1 cut(s)	312
BsaXI	ACNNNNNCTCC	4 cut(s)	1297, 1327, 1400, 1430
Bsc4I	CCNNNNNNNGG	7 cut(s)	379, 380, 381, 861, 918, 919, 1179
Bse118I	RCCGGY	4 cut(s)	579, 610, 898, 982
Bse1I	ACTGG	7 cut(s)	176, 555, 611, 1040, 1162, 1553, 1879
Bse3DI	GCAATG	1 cut(s)	1366
BseAI	TCCGGA	1 cut(s)	312
BseBI	CCWGG	2 cut(s)	642, 1137
BseGI	GGATG	3 cut(s)	559, 1378, 1539
BseLI	CCNNNNNNNGG	7 cut(s)	379, 380, 381, 861, 918, 919, 1179
BseMI	GCAATG	1 cut(s)	1366
BseMII	CTCAG	2 cut(s)	1140, 1601
BseNI	ACTGG	7 cut(s)	176, 555, 611, 1040, 1162, 1553, 1879
BseRI	GAGGAG	3 cut(s)	226, 386, 1399
BseSI	GKGCMC	1 cut(s)	803
BseX3I	CGGCCG	1 cut(s)	564
BseXI	GCAGC	8 cut(s)	31, 576, 604, 984, 1057, 1276, 1564, 1789
Bsh1236I	CGCG	9 cut(s)	357, 486, 510, 519, 536, 818, 935, 1175, 1626
Bsh1285I	CGRYCG	3 cut(s)	469, 567, 1236
BshFI	GGCC	8 cut(s)	5, 566, 610, 851, 1039, 1342, 1513, 1855
BshNI	GGYRCC	1 cut(s)	1108
BsiEI	CGRYCG	3 cut(s)	469, 567, 1236
BsiHKAI	GWGCWC	1 cut(s)	417
BsiHKCI	CYCGRG	2 cut(s)	218, 379
BsiWI	CGTACG	1 cut(s)	813
BslFI	GGGAC	6 cut(s)	422, 895, 1174, 1464, 1574, 1843
BslI	CCNNNNNNNGG	7 cut(s)	379, 380, 381, 861, 918, 919, 1179
BsmAI	GTCTC	2 cut(s)	630, 1232
BsmFI	GGGAC	6 cut(s)	422, 895, 1174, 1464, 1574, 1843
BsnI	GGCC	8 cut(s)	5, 566, 610, 851, 1039, 1342, 1513, 1855
Bso31I	GGTCTC	1 cut(s)	630
BsoBI	CYCGRG	2 cut(s)	218, 379
Bsp119I	TTCGAA	1 cut(s)	1661
Bsp1286I	GDGCHC	3 cut(s)	269, 417, 803
Bsp13I	TCCGGA	1 cut(s)	312
Bsp1407I	TGTACA	2 cut(s)	1224, 1347
BspANI	GGCC	8 cut(s)	5, 566, 610, 851, 1039, 1342, 1513, 1855
BspCNI	CTCAG	2 cut(s)	1141, 1602
BspEI	TCCGGA	1 cut(s)	312
BspFNI	CGCG	9 cut(s)	357, 486, 510, 519, 536, 818, 935, 1175, 1626
BspHI	TCATGA	2 cut(s)	663, 1024
BspLI	GGNNCC	6 cut(s)	268, 437, 438, 1110, 1184, 1410
BspMAI	CTGCAG	1 cut(s)	594
BspMI	ACCTGC	1 cut(s)	1751
BspPI	GGATC	7 cut(s)	291, 323, 392, 442, 966, 1584, 1802
BspT104I	TTCGAA	1 cut(s)	1661
BspT107I	GGYRCC	1 cut(s)	1108
BspTNI	GGTCTC	1 cut(s)	630
BsrBI	CCGCTC	1 cut(s)	1574
BsrDI	GCAATG	1 cut(s)	1366
BsrFI	RCCGGY	4 cut(s)	579, 610, 898, 982
BsrGI	TGTACA	2 cut(s)	1224, 1347
BsrI	ACTGG	7 cut(s)	176, 555, 611, 1040, 1162, 1553, 1879
BssAI	RCCGGY	4 cut(s)	579, 610, 898, 982
BssNAI	GTATAC	1 cut(s)	1644
BssNI	GRCGYC	2 cut(s)	660, 809
Bst1107I	GTATAC	1 cut(s)	1644
Bst2UI	CCWGG	2 cut(s)	642, 1137
Bst4CI	ACNGT	1 cut(s)	1588
BstACI	GRCGYC	2 cut(s)	660, 809
BstAPI	GCANNNNNTGC	1 cut(s)	1766
BstAUI	TGTACA	2 cut(s)	1224, 1347
BstBAI	YACGTR	1 cut(s)	572
BstBI	TTCGAA	1 cut(s)	1661
BstC8I	GCNNGC	5 cut(s)	417, 612, 900, 1173, 1262
BstDEI	CTNAG	2 cut(s)	1149, 1610
BstDSI	CCRYGG	2 cut(s)	1049, 1624
BstF5I	GGATG	3 cut(s)	559, 1378, 1539
BstFNI	CGCG	9 cut(s)	357, 486, 510, 519, 536, 818, 935, 1175, 1626
BstHHI	GCGC	4 cut(s)	486, 521, 536, 1171
BstMAI	GTCTC	2 cut(s)	630, 1232
BstMCI	CGRYCG	3 cut(s)	469, 567, 1236
BstMWI	GCNNNNNNNGC	6 cut(s)	516, 1045, 1054, 1408, 1561, 1766
BstNI	CCWGG	2 cut(s)	642, 1137
BstNSI	RCATGY	3 cut(s)	601, 1135, 1457
BstSCI	CCNGG	8 cut(s)	143, 257, 378, 379, 438, 501, 640, 1135
BstSFI	CTRYAG	1 cut(s)	590
BstSLI	GKGCMC	1 cut(s)	803
BstUI	CGCG	9 cut(s)	357, 486, 510, 519, 536, 818, 935, 1175, 1626
BstV1I	GCAGC	8 cut(s)	31, 576, 604, 984, 1057, 1276, 1564, 1789
BstV2I	GAAGAC	1 cut(s)	1316
BstX2I	RGATCY	2 cut(s)	315, 447
BstYI	RGATCY	2 cut(s)	315, 447
BstZ17I	GTATAC	1 cut(s)	1644
BstZI	CGGCCG	1 cut(s)	564
BsuRI	GGCC	8 cut(s)	5, 566, 610, 851, 1039, 1342, 1513, 1855
BtgI	CCRYGG	2 cut(s)	1049, 1624
BtrI	CACGTC	2 cut(s)	405, 559
BtsCI	GGATG	3 cut(s)	559, 1378, 1539
BtsIMutI	CAGTG	1 cut(s)	1607
BveI	ACCTGC	1 cut(s)	1751
Cac8I	GCNNGC	5 cut(s)	417, 612, 900, 1173, 1262
CaiI	CAGNNNCTG	1 cut(s)	1609
CciI	TCATGA	2 cut(s)	663, 1024
CciNI	GCGGCCGC	1 cut(s)	564
CfoI	GCGC	4 cut(s)	486, 521, 536, 1171
Cfr10I	RCCGGY	4 cut(s)	579, 610, 898, 982
Cfr13I	GGNCC	6 cut(s)	141, 327, 436, 442, 505, 1511
Cfr42I	CCGCGG	1 cut(s)	1627
Cfr9I	CCCGGG	1 cut(s)	379
CpoI	CGGWCCG	1 cut(s)	505
CseI	GACGC	4 cut(s)	475, 613, 649, 817
Csp6I	GTAC	6 cut(s)	223, 583, 814, 1225, 1348, 1621
CspI	CGGWCCG	1 cut(s)	505
CviQI	GTAC	6 cut(s)	223, 583, 814, 1225, 1348, 1621
DdeI	CTNAG	2 cut(s)	1149, 1610
DrdI	GACNNNNNNGTC	1 cut(s)	106
DriI	GACNNNNNGTC	2 cut(s)	157, 1207
DseDI	GACNNNNNNGTC	1 cut(s)	106
EaeI	YGGCCR	5 cut(s)	3, 564, 608, 849, 1037
EagI	CGGCCG	1 cut(s)	564
Eam1105I	GACNNNNNGTC	2 cut(s)	157, 1207
Ecl136II	GAGCTC	1 cut(s)	415
EclXI	CGGCCG	1 cut(s)	564
Eco24I	GRGCYC	2 cut(s)	269, 417
Eco31I	GGTCTC	1 cut(s)	630
Eco47I	GGWCC	5 cut(s)	141, 327, 436, 442, 505
Eco52I	CGGCCG	1 cut(s)	564
Eco53kI	GAGCTC	1 cut(s)	415
Eco72I	CACGTG	1 cut(s)	572
Eco88I	CYCGRG	2 cut(s)	218, 379
EcoICRI	GAGCTC	1 cut(s)	415
EcoO109I	RGGNCCY	2 cut(s)	436, 1511
EcoRII	CCWGG	2 cut(s)	640, 1135
EcoT38I	GRGCYC	2 cut(s)	269, 417
FalI	AAGNNNNNCTT	2 cut(s)	1656, 1688
FaqI	GGGAC	6 cut(s)	422, 895, 1174, 1464, 1574, 1843
FauI	CCCGC	1 cut(s)	1180
FauNDI	CATATG	1 cut(s)	831
FbaI	TGATCA	2 cut(s)	132, 285
FblI	GTMKAC	2 cut(s)	150, 1643
FokI	GGATG	3 cut(s)	566, 1385, 1546
FriOI	GRGCYC	2 cut(s)	269, 417
FspBI	CTAG	5 cut(s)	1068, 1164, 1179, 1569, 1647
GlaI	GCGC	4 cut(s)	485, 520, 535, 1170
GsuI	CTGGAG	1 cut(s)	223
HaeIII	GGCC	8 cut(s)	5, 566, 610, 851, 1039, 1342, 1513, 1855
HgaI	GACGC	4 cut(s)	475, 613, 649, 817
HhaI	GCGC	4 cut(s)	486, 521, 536, 1171
Hin1I	GRCGYC	2 cut(s)	660, 809
Hin6I	GCGC	4 cut(s)	484, 519, 534, 1169
HinP1I	GCGC	4 cut(s)	484, 519, 534, 1169
HincII	GTYRAC	2 cut(s)	151, 1155
HindII	GTYRAC	2 cut(s)	151, 1155
HindIII	AAGCTT	1 cut(s)	1670
HphI	GGTGA	5 cut(s)	803, 1571, 1643, 1732, 1766
Hpy166II	GTNNAC	7 cut(s)	151, 652, 1155, 1225, 1350, 1423, 1644
Hpy188III	TCNNGA	8 cut(s)	313, 347, 496, 653, 664, 750, 1025, 1737
Hpy8I	GTNNAC	7 cut(s)	151, 652, 1155, 1225, 1350, 1423, 1644
HpyAV	CCTTC	2 cut(s)	319, 1362
HpyCH4III	ACNGT	1 cut(s)	1588
HpyCH4IV	ACGT	4 cut(s)	404, 558, 571, 821
HpyCH4V	TGCA	6 cut(s)	592, 835, 1014, 1045, 1216, 1711
HpyF10VI	GCNNNNNNNGC	6 cut(s)	516, 1045, 1054, 1408, 1561, 1766
HpyF3I	CTNAG	2 cut(s)	1149, 1610
HpySE526I	ACGT	4 cut(s)	404, 558, 571, 821
Hsp92I	GRCGYC	2 cut(s)	660, 809
HspAI	GCGC	4 cut(s)	484, 519, 534, 1169
KflI	GGGWCCC	1 cut(s)	436
Kpn2I	TCCGGA	1 cut(s)	312
KroI	GCCGGC	2 cut(s)	610, 898
KroNI	GCCGGC	2 cut(s)	612, 900
Ksp22I	TGATCA	2 cut(s)	132, 285
KspI	CCGCGG	1 cut(s)	1627
LmnI	GCTCC	5 cut(s)	16, 272, 412, 1399, 1408
Lsp1109I	GCAGC	8 cut(s)	31, 576, 604, 984, 1057, 1276, 1564, 1789
LweI	GCATC	2 cut(s)	471, 1363
MaeI	CTAG	5 cut(s)	1068, 1164, 1179, 1569, 1647
MaeII	ACGT	4 cut(s)	404, 558, 571, 821
MaeIII	GTNAC	7 cut(s)	198, 496, 791, 872, 1249, 1432, 1720
MbiI	CCGCTC	1 cut(s)	1574
MboII	GAAGA	5 cut(s)	22, 25, 310, 958, 1321
MflI	RGATCY	2 cut(s)	315, 447
MhlI	GDGCHC	3 cut(s)	269, 417, 803
MlsI	TGGCCA	1 cut(s)	851
MluCI	AATT	2 cut(s)	1243, 1489
MluNI	TGGCCA	1 cut(s)	851
MlyI	GAGTC	6 cut(s)	116, 146, 341, 1160, 1306, 1429
MmeI	TCCRAC	6 cut(s)	147, 324, 704, 789, 1669, 1852
Mox20I	TGGCCA	1 cut(s)	851
MroI	TCCGGA	1 cut(s)	312
MroNI	GCCGGC	2 cut(s)	610, 898
MscI	TGGCCA	1 cut(s)	851
MseI	TTAA	2 cut(s)	92, 1299
MslI	CAYNNNNRTG	2 cut(s)	494, 1029
Msp20I	TGGCCA	1 cut(s)	851
MspA1I	CMGCKG	2 cut(s)	324, 1626
MspR9I	CCNGG	8 cut(s)	145, 259, 380, 381, 440, 503, 642, 1137
MvaI	CCWGG	2 cut(s)	642, 1137
MvnI	CGCG	9 cut(s)	357, 486, 510, 519, 536, 818, 935, 1175, 1626
MwoI	GCNNNNNNNGC	6 cut(s)	516, 1045, 1054, 1408, 1561, 1766
NaeI	GCCGGC	2 cut(s)	612, 900
NciI	CCSGG	6 cut(s)	145, 259, 380, 381, 440, 503
NdeI	CATATG	1 cut(s)	831
NgoMIV	GCCGGC	2 cut(s)	610, 898
NlaIV	GGNNCC	6 cut(s)	268, 437, 438, 1110, 1184, 1410
NmeAIII	GCCGAG	1 cut(s)	867
NmuCI	GTSAC	7 cut(s)	198, 496, 791, 872, 1249, 1432, 1720
NotI	GCGGCCGC	1 cut(s)	564
NspI	RCATGY	3 cut(s)	601, 1135, 1457
NspV	TTCGAA	1 cut(s)	1661
PagI	TCATGA	2 cut(s)	663, 1024
PaqCI	CACCTGC	1 cut(s)	1751
PasI	CCCWGGG	1 cut(s)	641
PciI	ACATGT	2 cut(s)	597, 1453
PcsI	WCGNNNNNNNCGW	6 cut(s)	95, 107, 194, 483, 815, 998
PdiI	GCCGGC	2 cut(s)	612, 900
PfeI	GAWTC	9 cut(s)	180, 425, 616, 761, 865, 1021, 1715, 1726, 1875
Pfl23II	CGTACG	1 cut(s)	813
PflMI	CCANNNNNTGG	1 cut(s)	861
PfoI	TCCNGGA	1 cut(s)	438
PleI	GAGTC	6 cut(s)	116, 146, 340, 1159, 1306, 1429
PmaCI	CACGTG	1 cut(s)	572
PmlI	CACGTG	1 cut(s)	572
PpsI	GAGTC	6 cut(s)	116, 146, 340, 1159, 1306, 1429
Ppu21I	YACGTR	1 cut(s)	572
PpuMI	RGGWCCY	1 cut(s)	436
PscI	ACATGT	2 cut(s)	597, 1453
PsiI	TTATAA	2 cut(s)	44, 1277
Psp124BI	GAGCTC	1 cut(s)	417
Psp5II	RGGWCCY	1 cut(s)	436
Psp6I	CCWGG	2 cut(s)	640, 1135
PspCI	CACGTG	1 cut(s)	572
PspGI	CCWGG	2 cut(s)	640, 1135
PspLI	CGTACG	1 cut(s)	813
PspN4I	GGNNCC	6 cut(s)	268, 437, 438, 1110, 1184, 1410
PspPI	GGNCC	6 cut(s)	141, 327, 436, 442, 505, 1511
PspPPI	RGGWCCY	1 cut(s)	436
PstI	CTGCAG	1 cut(s)	594
PstNI	CAGNNNCTG	1 cut(s)	1609
PsuI	RGATCY	2 cut(s)	315, 447
RsaI	GTAC	6 cut(s)	224, 584, 815, 1226, 1349, 1622
RsaNI	GTAC	6 cut(s)	223, 583, 814, 1225, 1348, 1621
RseI	CAYNNNNRTG	2 cut(s)	494, 1029
Rsr2I	CGGWCCG	1 cut(s)	505
RsrII	CGGWCCG	1 cut(s)	505
SacI	GAGCTC	1 cut(s)	417
SacII	CCGCGG	1 cut(s)	1627
SalI	GTCGAC	1 cut(s)	149
SaqAI	TTAA	2 cut(s)	92, 1299
Sau96I	GGNCC	6 cut(s)	141, 327, 436, 442, 505, 1511
SchI	GAGTC	6 cut(s)	116, 146, 341, 1160, 1306, 1429
ScrFI	CCNGG	8 cut(s)	145, 259, 380, 381, 440, 503, 642, 1137
SduI	GDGCHC	3 cut(s)	269, 417, 803
SfaNI	GCATC	2 cut(s)	471, 1363
SfcI	CTRYAG	1 cut(s)	590
Sfr303I	CCGCGG	1 cut(s)	1627
SfuI	TTCGAA	1 cut(s)	1661
SgrBI	CCGCGG	1 cut(s)	1627
SinI	GGWCC	5 cut(s)	141, 327, 436, 442, 505
SmaI	CCCGGG	1 cut(s)	381
SmiMI	CAYNNNNRTG	2 cut(s)	494, 1029
SpeI	ACTAGT	1 cut(s)	1163
Sse9I	AATT	2 cut(s)	1243, 1489
SspMI	CTAG	5 cut(s)	1068, 1164, 1179, 1569, 1647
SstI	GAGCTC	1 cut(s)	417
StyD4I	CCNGG	8 cut(s)	143, 257, 378, 379, 438, 501, 640, 1135
TaaI	ACNGT	1 cut(s)	1588
TaiI	ACGT	4 cut(s)	407, 561, 574, 824
TaqI	TCGA	9 cut(s)	60, 110, 150, 161, 474, 749, 1073, 1232, 1661
TaqII	GACCGA	1 cut(s)	1250
TasI	AATT	2 cut(s)	1243, 1489
TatI	WGTACW	2 cut(s)	1224, 1347
TauI	GCSGC	4 cut(s)	19, 193, 566, 569
TfiI	GAWTC	9 cut(s)	180, 425, 616, 761, 865, 1021, 1715, 1726, 1875
Tru1I	TTAA	2 cut(s)	92, 1299
Tru9I	TTAA	2 cut(s)	92, 1299
TscAI	CASTG	1 cut(s)	1614
TseFI	GTSAC	7 cut(s)	198, 496, 791, 872, 1249, 1432, 1720
TseI	GCWGC	8 cut(s)	19, 589, 592, 997, 1045, 1264, 1552, 1802
Tsp45I	GTSAC	7 cut(s)	198, 496, 791, 872, 1249, 1432, 1720
TspDTI	ATGAA	4 cut(s)	772, 818, 1448, 1508
TspGWI	ACGGA	2 cut(s)	561, 1470
TspMI	CCCGGG	1 cut(s)	379
TspRI	CASTG	1 cut(s)	1614
Van91I	CCANNNNNTGG	1 cut(s)	861
VpaK11BI	GGWCC	5 cut(s)	141, 327, 436, 442, 505
XceI	RCATGY	3 cut(s)	601, 1135, 1457
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1868
XmaI	CCCGGG	1 cut(s)	379
XmiI	GTMKAC	2 cut(s)	150, 1643
XspI	CTAG	5 cut(s)	1068, 1164, 1179, 1569, 1647

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.