Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0010093

Physical Location & Seq

Genomic Coordinates Reverse (-)

4484193 .. 4486861

Gene Structure 2669 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1064900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1974 bp

ATGGCCGTTCCTTCAACAACCTTCGTTTTCATCCTCCTCCTCTTCACCTTCTTCCCTCCGACCCACCAAACTGTTTCACTCTCTCTCTCCCCAACCTCCCTCTCCAAATCGGGCGACTCCGTCCTAATCCAATGGTCCGGCATCGACTCGCCCTCCCGACTCGACTGGCTCGGCATCTACTCGCCCCCTTCCTCCCACCACGACAACTTTATAGGATACAAGTTCCTCTCCTCCGCCCCCACATGGAAATCTGGGTCCGGCTCCATTTCCCTCCCTCTGGTCAATCTCCGATTGAACTACTCCTTCCGGATTTTCAGCTGGAACGAGTCCGAGGTCGACCCCAACCACGTCGACCAGGACCACAACCCCCTCCCCGGCACCGCCCACCTGCTCGCCACCTCAGATGACGAGCTTAGCTTCGAATCGGGTCGTGTTCCGGATCAGATCCACCTCGCTTACACGGACGGAGATGACGAGATGCGGGTCATGTTCGTGACCCCAGACGGCGGGGAGAGAAAGGTTCGATACGGTCAGAGGGAGGACTCGCTCGATGACGTGGTGGTGGCACGTGTGGGGACGTATGAAAGGGAGCACATGTGCGATGCACCCGCCAACAACAGTATCGGGTGGAGAGATCCCGGGTTTATCCACGATGCGGTCATGACCCGACTGGGAAAAGGCGTCAGATATTATTACAAGGTCGGAAGTGATTCTGAAGGATGGAGCAAAACGCACAGCTTTGTCGCACGAAACGAGGACTCGGACGAAACTACGGCCTTTATGTTTGGTGACATGGGGACTGCAACACCTTATGCAACGTTTCTTCGGACACAAGACGAGAGCATAGCCACTGTGAAATGGATCCTCCGGGATGTTGAAGCTCTTGGTGAAAAGCCCGCTTTTGTGTCCCACATCGGAGACATAAGCTACGCGAGAGGGTACTCGTGGTTGTGGGATCAATTCTTCATCCAAATTGAGCCCCTTGCATCCAAGCTTCCATACCATGTTTGTATTGGAAATCACGAGTATGATTGGCCGTTGCAGCCATGGAAACCGGAGTGGGCAACAATGTATGGGACAGATGGTGGGGGAGAATGTGGGGTGCCCTACAGTCTTAAGTTCAACATGCCAGGAAACTCATCTGAACCGACTGGAACAAGTGCCCCAGCTACACGAAATCTTTACTATTCGTTTGATGTTGGGTCGGTACATTTTGTGTACATTTCAACTGAGACCAATTTTGTTGAAGGAAGCAAGCAATTGGAATTTATTAAGCGGGATTTGGAGGCGGTGGATAGGAGGAAGACGCCATTTGTGGTGGTGCAAGGACATAGGCCAATGTACACAACAAGCTACGAGAGAGGTGATGCTCCTTTGAGGGTGAAAATGCTGGAGCATTTGGAGCCTCTGTTTGTGAAGAACAATGTAAATCTTGCACTATGGGGACATGTACACAGATACGAGAGGTTTTGTCAGTTGGTAAACTATACTTGTGGGAGTGTTGGACCTATTCATGTTGTGATTGGGATGGGTGGCCAAGACTGGCAACCGATCTGGGAGCCTAGACCAGATCATCCAACTGACCCAGTGTACCCACAGCCAGAGCGGTCTTTATACCGCGGTGGTGAATTTGGGTACACTAGGTTGGTTGCCACAAGGAAGAAGCTTACACTTACTTATGTTGGAAACCATGATGGGAAGGTGCACGACACGGTGGAGATTCTGGCTTCAGGCCAAGTTCTCGGCGGTAGTGGAGCCGGTGGAGGCGGTATCAAGTCTGTTAATATCGGTAGCAGTGGTGATGCTGGGGTTATTAGGAGCAGTGGGGAGTCTACATTCTCTTGGTTTGTGAAGGGAGCAAGTGTGTTGGTGCTTGGGGTTTTTATAGGATACGTTGTGGGTTACATTTCATATTCCAGGAAAAAGGCCGTTCCAGGAAGTAACTGGACTCCTGTGAAGAGTGAGGAAACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0005048 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005768 GO:0005794 GO:0005802 GO:0006109 GO:0006605 GO:0006626 GO:0006793 GO:0006796 GO:0006810 GO:0006839 GO:0006886 GO:0006996 GO:0007005 GO:0007006 GO:0008104 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0017038 GO:0019222 GO:0030943 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0031984 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0042277 GO:0042578 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044429 GO:0044431 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045040 GO:0045184 GO:0046907 GO:0050789 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061024 GO:0065007 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0072594 GO:0072655 GO:0072657 GO:0080090 GO:0090150 GO:0090151 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

658

Amino Acids

73.11

Weight (kDa)

5.71

Isoelectric Point (pI)

35.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pur_ac_phosph_N	PF16656	146 - 248	1e-19	Purple acid Phosphatase, N-terminal domain
Metallophos	PF00149	263 - 487	1.9e-13	Calcineurin-like phosphoesterase
Metallophos_C	PF14008	502 - 571	4.4e-15	Iron/zinc purple acid phosphatase-like protein C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010093)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13900 AT2G03450
fragaria_vesca	FvH4_4g30400 FvH4_4g30400
malus_domestica	MD13G1064900.v1.1
prunus_persica	Prupe.1G283200_v2.0.a1 Prupe.1G283200_v2.0.a1
pyrus_communis	pycom13g05730
rosa_chinensis	RchiOBHm_Chr4g0438691
rosa_laevigata	RLG00000006337
rosa_multiflora	Rmu_co8427227.1_g000001 Rmu_sc0000738.1_g000044 Rmu_sc0012315.1_g000001
rosa_roxburghii	Rroxscaffold_5G00379730
rosa_rugosa	Rorug04G0312700
rosa_samantha	Rh4AG363200 Rh4BG374600 Rh4CG388800 Rh4DG369100
rosa_wichuraiana	Rw0G007170 Rw0G016710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	396
Acc36I	ACCTGC	1 cut(s)	396
AccB1I	GGYRCC	2 cut(s)	377, 1102
AccBSI	CCGCTC	1 cut(s)	1606
AccI	GTMKAC	3 cut(s)	336, 351, 1832
AccII	CGCG	2 cut(s)	932, 1620
AccIII	TCCGGA	2 cut(s)	306, 436
AclI	AACGTT	1 cut(s)	818
AclWI	GGATC	6 cut(s)	439, 447, 629, 856, 869, 963
AcoI	YGGCCR	3 cut(s)	3, 1034, 1534
AcsI	RAATTY	2 cut(s)	1265, 1628
AcuI	CTGAAG	2 cut(s)	735, 1713
AcvI	CACGTG	1 cut(s)	569
AcyI	GRCGYC	2 cut(s)	681, 1307
AfaI	GTAC	7 cut(s)	941, 1209, 1220, 1343, 1452, 1592, 1637
AfiI	CCNNNNNNNGG	5 cut(s)	243, 277, 374, 506, 655
AflII	CTTAAG	1 cut(s)	1115
AflIII	ACRYGT	3 cut(s)	568, 594, 1447
AgsI	TTSAA	6 cut(s)	15, 295, 878, 1123, 1227, 1247
AjiI	CACGTC	2 cut(s)	349, 556
AjnI	CCWGG	4 cut(s)	354, 1129, 1916, 1933
Alw21I	GWGCWC	2 cut(s)	594, 1707
Alw26I	GTCTC	2 cut(s)	912, 1226
Alw44I	GTGCAC	1 cut(s)	1703
AlwI	GGATC	6 cut(s)	439, 447, 629, 856, 869, 963
Ama87I	CYCGRG	1 cut(s)	638
Aor13HI	TCCGGA	2 cut(s)	306, 436
AoxI	GGCC	7 cut(s)	3, 774, 1034, 1334, 1534, 1732, 1926
ApaLI	GTGCAC	1 cut(s)	1703
ApeKI	GCWGC	1 cut(s)	1042
ApoI	RAATTY	2 cut(s)	1265, 1628
Asp700I	GAANNNNTTC	1 cut(s)	709
AspS9I	GGNCC	4 cut(s)	135, 255, 358, 1505
AsuC2I	CCSGG	4 cut(s)	375, 639, 640, 869
AsuHPI	GGTGA	7 cut(s)	37, 800, 899, 1376, 1393, 1637, 1811
AsuII	TTCGAA	1 cut(s)	420
AvaI	CYCGRG	1 cut(s)	638
AvaII	GGWCC	4 cut(s)	135, 255, 358, 1505
BaeGI	GKGCMC	3 cut(s)	1107, 1165, 1707
BaeI	ACNNNNGTAYC	2 cut(s)	604, 637
BalI	TGGCCA	1 cut(s)	1536
BamHI	GGATCC	1 cut(s)	861
BanI	GGYRCC	2 cut(s)	377, 1102
BanII	GRGCYC	1 cut(s)	981
BauI	CACGAG	2 cut(s)	943, 1022
BbrPI	CACGTG	1 cut(s)	569
BbsI	GAAGAC	1 cut(s)	1310
Bbv12I	GWGCWC	2 cut(s)	594, 1707
BbvI	GCAGC	1 cut(s)	1054
BccI	CCATC	4 cut(s)	714, 1076, 1522, 1688
BceAI	ACGGC	4 cut(s)	520, 789, 1021, 1913
BciT130I	CCWGG	4 cut(s)	356, 1131, 1918, 1935
BciVI	GTATCC	2 cut(s)	209, 1883
BcnI	CCSGG	4 cut(s)	375, 639, 640, 869
BcoDI	GTCTC	2 cut(s)	912, 1226
BfaI	CTAG	2 cut(s)	1563, 1641
BfmI	CTRYAG	1 cut(s)	1108
BfrI	CTTAAG	1 cut(s)	1115
BfuAI	ACCTGC	1 cut(s)	396
BfuI	GTATCC	2 cut(s)	209, 1883
BisI	GCNGC	1 cut(s)	1043
BlpI	GCTNAGC	1 cut(s)	413
BlsI	GCNGC	1 cut(s)	1044
Bme1390I	CCNGG	8 cut(s)	356, 375, 639, 640, 869, 1131, 1918, 1935
Bme18I	GGWCC	4 cut(s)	135, 255, 358, 1505
BmeT110I	CYCGRG	1 cut(s)	638
BmgBI	CACGTC	2 cut(s)	349, 556
BmgT120I	GGNCC	4 cut(s)	135, 255, 358, 1505
BmiI	GGNNCC	8 cut(s)	256, 262, 379, 863, 1104, 1404, 1560, 1756
BmrFI	CCNGG	8 cut(s)	356, 375, 639, 640, 869, 1131, 1918, 1935
BmrI	ACTGGG	2 cut(s)	680, 1580
BmsI	GCATC	8 cut(s)	150, 183, 468, 592, 643, 995, 1357, 1792
BmuI	ACTGGG	2 cut(s)	680, 1580
BpiI	GAAGAC	1 cut(s)	1310
BpmI	CTGGAG	1 cut(s)	1412
Bpu1102I	GCTNAGC	1 cut(s)	413
Bpu14I	TTCGAA	1 cut(s)	420
BpuMI	CCSGG	4 cut(s)	375, 639, 640, 869
BsaAI	YACGTR	1 cut(s)	569
BsaHI	GRCGYC	2 cut(s)	681, 1307
BsaI	GGTCTC	1 cut(s)	1226
BsaJI	CCNNGG	5 cut(s)	330, 373, 638, 1046, 1618
BsaWI	WCCGGW	3 cut(s)	306, 436, 1054
BsaXI	ACNNNNNCTCC	8 cut(s)	137, 167, 581, 611, 1394, 1424, 1848, 1878
Bsc4I	CCNNNNNNNGG	5 cut(s)	243, 277, 374, 506, 655
Bse118I	RCCGGY	1 cut(s)	1757
Bse1I	ACTGG	6 cut(s)	170, 675, 1156, 1547, 1586, 1949
BseAI	TCCGGA	2 cut(s)	306, 436
BseBI	CCWGG	4 cut(s)	356, 1131, 1918, 1935
BseDI	CCNNGG	5 cut(s)	330, 373, 638, 1046, 1618
BseGI	GGATG	7 cut(s)	30, 725, 877, 966, 986, 1533, 1573
BseLI	CCNNNNNNNGG	5 cut(s)	243, 277, 374, 506, 655
BseMII	CTCAG	2 cut(s)	414, 1221
BseNI	ACTGG	6 cut(s)	170, 675, 1156, 1547, 1586, 1949
BseRI	GAGGAG	3 cut(s)	26, 29, 220
BseSI	GKGCMC	3 cut(s)	1107, 1165, 1707
BseXI	GCAGC	1 cut(s)	1054
BseYI	CCCAGC	2 cut(s)	1165, 1805
Bsh1236I	CGCG	2 cut(s)	932, 1620
BshFI	GGCC	7 cut(s)	5, 776, 1036, 1336, 1536, 1734, 1928
BshNI	GGYRCC	2 cut(s)	377, 1102
BsiHKAI	GWGCWC	2 cut(s)	594, 1707
BsiHKCI	CYCGRG	1 cut(s)	638
BsiSI	CCGG	9 cut(s)	138, 258, 307, 375, 437, 639, 868, 1055, 1758
BslFI	GGGAC	5 cut(s)	589, 811, 892, 1090, 1458
BslI	CCNNNNNNNGG	5 cut(s)	243, 277, 374, 506, 655
BsmAI	GTCTC	2 cut(s)	912, 1226
BsmFI	GGGAC	5 cut(s)	589, 811, 892, 1090, 1458
BsnI	GGCC	7 cut(s)	5, 776, 1036, 1336, 1536, 1734, 1928
Bso31I	GGTCTC	1 cut(s)	1226
BsoBI	CYCGRG	1 cut(s)	638
Bsp119I	TTCGAA	1 cut(s)	420
Bsp1286I	GDGCHC	5 cut(s)	594, 981, 1107, 1165, 1707
Bsp13I	TCCGGA	2 cut(s)	306, 436
Bsp1407I	TGTACA	3 cut(s)	1218, 1341, 1450
Bsp143I	GATC	7 cut(s)	439, 444, 634, 861, 955, 1551, 1570
Bsp1720I	GCTNAGC	1 cut(s)	413
Bsp19I	CCATGG	1 cut(s)	1046
BspANI	GGCC	7 cut(s)	5, 776, 1036, 1336, 1536, 1734, 1928
BspCNI	CTCAG	2 cut(s)	413, 1222
BspEI	TCCGGA	2 cut(s)	306, 436
BspFNI	CGCG	2 cut(s)	932, 1620
BspHI	TCATGA	1 cut(s)	660
BspLI	GGNNCC	8 cut(s)	256, 262, 379, 863, 1104, 1404, 1560, 1756
BspMI	ACCTGC	1 cut(s)	396
BspPI	GGATC	6 cut(s)	439, 447, 629, 856, 869, 963
BspT104I	TTCGAA	1 cut(s)	420
BspT107I	GGYRCC	2 cut(s)	377, 1102
BspTI	CTTAAG	1 cut(s)	1115
BspTNI	GGTCTC	1 cut(s)	1226
BsrBI	CCGCTC	1 cut(s)	1606
BsrFI	RCCGGY	1 cut(s)	1757
BsrGI	TGTACA	3 cut(s)	1218, 1341, 1450
BsrI	ACTGG	6 cut(s)	170, 675, 1156, 1547, 1586, 1949
BssAI	RCCGGY	1 cut(s)	1757
BssECI	CCNNGG	5 cut(s)	330, 373, 638, 1046, 1618
BssMI	GATC	7 cut(s)	439, 444, 634, 861, 955, 1551, 1570
BssNI	GRCGYC	2 cut(s)	681, 1307
BssSI	CACGAG	2 cut(s)	943, 1022
BssT1I	CCWWGG	1 cut(s)	1046
Bst2BI	CACGAG	2 cut(s)	943, 1022
Bst2UI	CCWGG	4 cut(s)	356, 1131, 1918, 1935
Bst4CI	ACNGT	6 cut(s)	73, 530, 620, 853, 1112, 1714
Bst6I	CTCTTC	2 cut(s)	47, 1952
BstACI	GRCGYC	2 cut(s)	681, 1307
BstAFI	CTTAAG	1 cut(s)	1115
BstAUI	TGTACA	3 cut(s)	1218, 1341, 1450
BstBAI	YACGTR	1 cut(s)	569
BstBI	TTCGAA	1 cut(s)	420
BstC8I	GCNNGC	3 cut(s)	393, 897, 1256
BstDEI	CTNAG	3 cut(s)	400, 413, 1230
BstDSI	CCRYGG	2 cut(s)	1046, 1618
BstF5I	GGATG	7 cut(s)	30, 725, 877, 966, 986, 1533, 1573
BstFNI	CGCG	2 cut(s)	932, 1620
BstKTI	GATC	7 cut(s)	442, 447, 637, 864, 958, 1554, 1573
BstMAI	GTCTC	2 cut(s)	912, 1226
BstMBI	GATC	7 cut(s)	439, 444, 634, 861, 955, 1551, 1570
BstMWI	GCNNNNNNNGC	2 cut(s)	1042, 1402
BstNI	CCWGG	4 cut(s)	356, 1131, 1918, 1935
BstNSI	RCATGY	3 cut(s)	598, 1129, 1451
BstSCI	CCNGG	8 cut(s)	354, 373, 637, 638, 867, 1129, 1916, 1933
BstSFI	CTRYAG	1 cut(s)	1108
BstSLI	GKGCMC	3 cut(s)	1107, 1165, 1707
BstUI	CGCG	2 cut(s)	932, 1620
BstV1I	GCAGC	1 cut(s)	1054
BstV2I	GAAGAC	1 cut(s)	1310
BstX2I	RGATCY	3 cut(s)	444, 634, 861
BstYI	RGATCY	3 cut(s)	444, 634, 861
BsuI	GTATCC	2 cut(s)	209, 1883
BsuRI	GGCC	7 cut(s)	5, 776, 1036, 1336, 1536, 1734, 1928
BtgI	CCRYGG	2 cut(s)	1046, 1618
BtgZI	GCGATG	1 cut(s)	615
BtrI	CACGTC	2 cut(s)	349, 556
BtsCI	GGATG	7 cut(s)	30, 725, 877, 966, 986, 1533, 1573
BtsI	GCAGTG	2 cut(s)	1801, 1828
BtsIMutI	CAGTG	4 cut(s)	849, 1593, 1801, 1828
BveI	ACCTGC	1 cut(s)	396
Cac8I	GCNNGC	3 cut(s)	393, 897, 1256
CciI	TCATGA	1 cut(s)	660
Cfr10I	RCCGGY	1 cut(s)	1757
Cfr13I	GGNCC	4 cut(s)	135, 255, 358, 1505
Cfr42I	CCGCGG	1 cut(s)	1621
Cfr9I	CCCGGG	1 cut(s)	638
CseI	GACGC	2 cut(s)	670, 1315
Csp6I	GTAC	7 cut(s)	940, 1208, 1219, 1342, 1451, 1591, 1636
CviQI	GTAC	7 cut(s)	940, 1208, 1219, 1342, 1451, 1591, 1636
DdeI	CTNAG	3 cut(s)	400, 413, 1230
DpnI	GATC	7 cut(s)	441, 446, 636, 863, 957, 1553, 1572
DpnII	GATC	7 cut(s)	439, 444, 634, 861, 955, 1551, 1570
EaeI	YGGCCR	3 cut(s)	3, 1034, 1534
Eam1104I	CTCTTC	2 cut(s)	47, 1952
EarI	CTCTTC	2 cut(s)	47, 1952
EciI	GGCGGA	1 cut(s)	223
Eco130I	CCWWGG	1 cut(s)	1046
Eco24I	GRGCYC	1 cut(s)	981
Eco31I	GGTCTC	1 cut(s)	1226
Eco47I	GGWCC	4 cut(s)	135, 255, 358, 1505
Eco57I	CTGAAG	2 cut(s)	735, 1713
Eco72I	CACGTG	1 cut(s)	569
Eco88I	CYCGRG	1 cut(s)	638
EcoRII	CCWGG	4 cut(s)	354, 1129, 1916, 1933
EcoT14I	CCWWGG	1 cut(s)	1046
EcoT38I	GRGCYC	1 cut(s)	981
ErhI	CCWWGG	1 cut(s)	1046
FaqI	GGGAC	5 cut(s)	589, 811, 892, 1090, 1458
FauI	CCCGC	5 cut(s)	474, 500, 616, 904, 1269
FblI	GTMKAC	3 cut(s)	336, 351, 1832
Fnu4HI	GCNGC	1 cut(s)	1043
FokI	GGATG	7 cut(s)	17, 732, 884, 953, 973, 1540, 1560
FriOI	GRGCYC	1 cut(s)	981
Fsp4HI	GCNGC	1 cut(s)	1043
FspBI	CTAG	2 cut(s)	1563, 1641
GluI	GCNGC	1 cut(s)	1043
GsaI	CCCAGC	2 cut(s)	1169, 1809
GsuI	CTGGAG	1 cut(s)	1412
HaeIII	GGCC	7 cut(s)	5, 776, 1036, 1336, 1536, 1734, 1928
HapII	CCGG	9 cut(s)	138, 258, 307, 375, 437, 639, 868, 1055, 1758
HgaI	GACGC	2 cut(s)	670, 1315
Hin1I	GRCGYC	2 cut(s)	681, 1307
HincII	GTYRAC	2 cut(s)	337, 352
HindII	GTYRAC	2 cut(s)	337, 352
HindIII	AAGCTT	2 cut(s)	992, 1664
HpaII	CCGG	9 cut(s)	138, 258, 307, 375, 437, 639, 868, 1055, 1758
HphI	GGTGA	7 cut(s)	37, 800, 899, 1376, 1393, 1637, 1811
Hpy188III	TCNNGA	6 cut(s)	156, 307, 437, 493, 661, 1022
Hpy99I	CGWCG	1 cut(s)	353
HpyAV	CCTTC	9 cut(s)	21, 31, 58, 198, 313, 710, 1241, 1693, 1846
HpyCH4III	ACNGT	6 cut(s)	73, 530, 620, 853, 1112, 1714
HpyCH4IV	ACGT	6 cut(s)	348, 555, 568, 578, 818, 1893
HpyCH4V	TGCA	8 cut(s)	605, 803, 815, 986, 1042, 1324, 1436, 1705
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1042, 1402
HpyF3I	CTNAG	3 cut(s)	400, 413, 1230
HpySE526I	ACGT	6 cut(s)	348, 555, 568, 578, 818, 1893
Hsp92I	GRCGYC	2 cut(s)	681, 1307
Kpn2I	TCCGGA	2 cut(s)	306, 436
KspI	CCGCGG	1 cut(s)	1621
Kzo9I	GATC	7 cut(s)	439, 444, 634, 861, 955, 1551, 1570
Lsp1109I	GCAGC	1 cut(s)	1054
LweI	GCATC	8 cut(s)	150, 183, 468, 592, 643, 995, 1357, 1792
MaeI	CTAG	2 cut(s)	1563, 1641
MaeII	ACGT	6 cut(s)	348, 555, 568, 578, 818, 1893
MaeIII	GTNAC	4 cut(s)	493, 788, 1901, 1940
MalI	GATC	7 cut(s)	441, 446, 636, 863, 957, 1553, 1572
MbiI	CCGCTC	1 cut(s)	1606
MboI	GATC	7 cut(s)	439, 444, 634, 861, 955, 1551, 1570
MboII	GAAGA	8 cut(s)	34, 43, 815, 955, 1315, 1429, 1672, 1969
MfeI	CAATTG	1 cut(s)	1259
MflI	RGATCY	3 cut(s)	444, 634, 861
MhlI	GDGCHC	5 cut(s)	594, 981, 1107, 1165, 1707
MlsI	TGGCCA	1 cut(s)	1536
MluCI	AATT	6 cut(s)	959, 972, 1237, 1259, 1265, 1628
MluNI	TGGCCA	1 cut(s)	1536
MlyI	GAGTC	8 cut(s)	110, 140, 153, 335, 536, 752, 1838, 1942
MmeI	TCCRAC	5 cut(s)	83, 682, 1483, 1601, 1663
Mox20I	TGGCCA	1 cut(s)	1536
MroI	TCCGGA	2 cut(s)	306, 436
MroXI	GAANNNNTTC	1 cut(s)	709
MscI	TGGCCA	1 cut(s)	1536
MseI	TTAA	3 cut(s)	1116, 1272, 1782
MslI	CAYNNNNRTG	2 cut(s)	491, 1026
Msp20I	TGGCCA	1 cut(s)	1536
MspA1I	CMGCKG	2 cut(s)	318, 1620
MspCI	CTTAAG	1 cut(s)	1115
MspI	CCGG	9 cut(s)	138, 258, 307, 375, 437, 639, 868, 1055, 1758
MspR9I	CCNGG	8 cut(s)	356, 375, 639, 640, 869, 1131, 1918, 1935
MunI	CAATTG	1 cut(s)	1259
MvaI	CCWGG	4 cut(s)	356, 1131, 1918, 1935
MvnI	CGCG	2 cut(s)	932, 1620
MwoI	GCNNNNNNNGC	2 cut(s)	1042, 1402
NciI	CCSGG	4 cut(s)	375, 639, 640, 869
NcoI	CCATGG	1 cut(s)	1046
NdeII	GATC	7 cut(s)	439, 444, 634, 861, 955, 1551, 1570
NlaIV	GGNNCC	8 cut(s)	256, 262, 379, 863, 1104, 1404, 1560, 1756
NmeAIII	GCCGAG	2 cut(s)	150, 1722
NmuCI	GTSAC	2 cut(s)	493, 788
NspI	RCATGY	3 cut(s)	598, 1129, 1451
NspV	TTCGAA	1 cut(s)	420
PagI	TCATGA	1 cut(s)	660
PaqCI	CACCTGC	1 cut(s)	396
PciI	ACATGT	2 cut(s)	594, 1447
PcsI	WCGNNNNNNNCGW	2 cut(s)	471, 750
PdmI	GAANNNNTTC	1 cut(s)	709
PfeI	GAWTC	3 cut(s)	422, 710, 1720
PflFI	GACNNNGTC	1 cut(s)	119
PfoI	TCCNGGA	3 cut(s)	867, 1916, 1933
PkrI	GCNGC	1 cut(s)	1044
PleI	GAGTC	8 cut(s)	110, 140, 153, 334, 536, 752, 1837, 1942
PmaCI	CACGTG	1 cut(s)	569
PmlI	CACGTG	1 cut(s)	569
PpsI	GAGTC	8 cut(s)	110, 140, 153, 334, 536, 752, 1837, 1942
Ppu21I	YACGTR	1 cut(s)	569
PscI	ACATGT	2 cut(s)	594, 1447
Psp1406I	AACGTT	1 cut(s)	818
Psp6I	CCWGG	4 cut(s)	354, 1129, 1916, 1933
PspCI	CACGTG	1 cut(s)	569
PspFI	CCCAGC	2 cut(s)	1165, 1805
PspGI	CCWGG	4 cut(s)	354, 1129, 1916, 1933
PspN4I	GGNNCC	8 cut(s)	256, 262, 379, 863, 1104, 1404, 1560, 1756
PspPI	GGNCC	4 cut(s)	135, 255, 358, 1505
PsuI	RGATCY	3 cut(s)	444, 634, 861
PsyI	GACNNNGTC	1 cut(s)	119
PvuII	CAGCTG	1 cut(s)	318
RsaI	GTAC	7 cut(s)	941, 1209, 1220, 1343, 1452, 1592, 1637
RsaNI	GTAC	7 cut(s)	940, 1208, 1219, 1342, 1451, 1591, 1636
RseI	CAYNNNNRTG	2 cut(s)	491, 1026
SacII	CCGCGG	1 cut(s)	1621
SalI	GTCGAC	2 cut(s)	335, 350
SaqAI	TTAA	3 cut(s)	1116, 1272, 1782
SatI	GCNGC	1 cut(s)	1043
Sau3AI	GATC	7 cut(s)	439, 444, 634, 861, 955, 1551, 1570
Sau96I	GGNCC	4 cut(s)	135, 255, 358, 1505
SchI	GAGTC	8 cut(s)	110, 140, 153, 335, 536, 752, 1838, 1942
ScrFI	CCNGG	8 cut(s)	356, 375, 639, 640, 869, 1131, 1918, 1935
SduI	GDGCHC	5 cut(s)	594, 981, 1107, 1165, 1707
SfaNI	GCATC	8 cut(s)	150, 183, 468, 592, 643, 995, 1357, 1792
SfcI	CTRYAG	1 cut(s)	1108
Sfr303I	CCGCGG	1 cut(s)	1621
SfuI	TTCGAA	1 cut(s)	420
SgrBI	CCGCGG	1 cut(s)	1621
SinI	GGWCC	4 cut(s)	135, 255, 358, 1505
SmaI	CCCGGG	1 cut(s)	640
SmiMI	CAYNNNNRTG	2 cut(s)	491, 1026
SmlI	CTYRAG	1 cut(s)	1115
SmoI	CTYRAG	1 cut(s)	1115
Sse9I	AATT	6 cut(s)	959, 972, 1237, 1259, 1265, 1628
SspMI	CTAG	2 cut(s)	1563, 1641
StyD4I	CCNGG	8 cut(s)	354, 373, 637, 638, 867, 1129, 1916, 1933
StyI	CCWWGG	1 cut(s)	1046
TaaI	ACNGT	6 cut(s)	73, 530, 620, 853, 1112, 1714
TaiI	ACGT	6 cut(s)	351, 558, 571, 581, 821, 1896
TaqI	TCGA	7 cut(s)	144, 162, 336, 351, 420, 523, 549
TasI	AATT	6 cut(s)	959, 972, 1237, 1259, 1265, 1628
TatI	WGTACW	3 cut(s)	1218, 1341, 1450
TfiI	GAWTC	3 cut(s)	422, 710, 1720
Tru1I	TTAA	3 cut(s)	1116, 1272, 1782
Tru9I	TTAA	3 cut(s)	1116, 1272, 1782
TscAI	CASTG	4 cut(s)	856, 1593, 1801, 1828
TseFI	GTSAC	2 cut(s)	493, 788
TseI	GCWGC	1 cut(s)	1042
Tsp45I	GTSAC	2 cut(s)	493, 788
TspDTI	ATGAA	5 cut(s)	19, 597, 955, 1502, 1899
TspGWI	ACGGA	3 cut(s)	109, 476, 480
TspMI	CCCGGG	1 cut(s)	638
TspRI	CASTG	4 cut(s)	856, 1593, 1801, 1828
Tth111I	GACNNNGTC	1 cut(s)	119
Vha464I	CTTAAG	1 cut(s)	1115
VneI	GTGCAC	1 cut(s)	1703
VpaK11BI	GGWCC	4 cut(s)	135, 255, 358, 1505
XapI	RAATTY	2 cut(s)	1265, 1628
XceI	RCATGY	3 cut(s)	598, 1129, 1451
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1010, 1941
XmaI	CCCGGG	1 cut(s)	638
XmiI	GTMKAC	3 cut(s)	336, 351, 1832
XmnI	GAANNNNTTC	1 cut(s)	709
XspI	CTAG	2 cut(s)	1563, 1641

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.