FvH4_4g30990

methyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018029
Physical Location & Seq
Forward (+)
30344914 .. 30346635
1722 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30990.t1

Sequence Viewer

Length: 1365 bp
ATGGACGCCGCAACAGCAACAACAGCAGCGGCCTCAACGATCAAAACACTTCACGCATTTTCGCCTTCTTCTACAACCCGTAAAATCTTGGCATTGCCTACTACCCGACCCCAAACTCTTTCTCTTTACAGATCTTTCCACACCCAACAATTCAAAACTCGTTCATTTACAAGATGCTCTACAAAGCCAGGAATCGACAGCAACAACGCGACCAACACAAACAAGTCCCTCGGGCTCGAGTCAAGTTCCCCAGCACAGCATCTGACGACTTCTCCGCCCAACCAAACCCTCACATCGACATGGTCAACGGGTCTAGTCTTTGATCTGGGTAACAAAACCTCGTGGGACAGCTCCGAAATCGGATCTCCCGTGGTGAAAAGGTTCATCGGCGACAATGAAGAGCGGTGGTACATGTGGTACCACGGTAGGTCGTCCGATCCGAACAACAGCTCCATCGGCCTCGCGGTTTCTAGTAATGGGATTCATTGGGCGAGAGGAGCCGAACACGTCATGTCTTGTGGTGAGGACGAGGGTTTGGTCATGAACTGCTCGAACAATTGGTGGGCGTTTGACACTAAGAGCATTAAGCCTTCCGAGATGGTCATCATGTCGAGTCCAATGTACAGCGCAGTGTACTGGCTTTACTACACCGGATACAGTTCGGAAAAGGTGGACTGTACTAACTATCCGGTTGGTCAATATCCGGTGGACGCACTCAAGTCTCTGCCGGGACTGGCTTGTAGCCAGGACGGGCGAAACTGGGCCAGAATCGAAGGGGATTATCACAGTGGTGCCTTGCTCGACGTAGGGTCCAAAAATGAGTGGGATTCTTTGTCCATTGCAAAACCTCAAGTGGTTGTGCATGGCAGCGATGACATGAGAATGTATTACCATTCCTTTGATGCAGAAAAAGGCCACTTTGCCATTGGGATGGCCAGATCCAGGGACGGAATAAGATGGGTGAAGTTGGGGAAAATTATGGGGGGAGGACCAAAGGGGTCTTTTGACGAGCTTGGGGTGAAAAATGCTTGTGTGGTTAGGAACAGGAAGGAAGGGAACTACCTAATGGCGTATGAGGGTGTGAGGGCAGATGGGAAGATAAGTATCGGTCTCGCTGTATCTCCCGATGGGCTGAACGAGTGGACGAGGGTACAAAACGGAGCGGTTCTTAGCCCATCGGGAGATGATGCATGGGACAACAAAGGAGTCGGCTCGCCAAGCCTGGTACAGATGGAAGGGAATGCAGACTTGTGGAGATTGTATTATGTGGGTGTCGGAGAAGATGGAAGGACTGGGATTGGGATGGCGATTTCGGAAGGTAGTGACGTGAGAACCTTCAGAAGGTGGGCAGGGTTTCAATCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

455

Amino Acids

49.68

Weight (kDa)

6.8

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018029)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g30990
rosa_chinensis RchiOBHm_Chr4g0439361
rosa_laevigata RLG00000006276
rosa_multiflora Rmu_co8431667.1_g000001 Rmu_sc0004012.1_g000003
rosa_roxburghii Rroxscaffold_5G00380290
rosa_samantha Rh4AG368800 Rh4BG380600 Rh4CG395100 Rh4DG375400
rosa_wichuraiana Rw4G031450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 417
AccB1I GGYRCC 2 cut(s) 417, 791
AccBSI CCGCTC 2 cut(s) 403, 1163
AccII CGCG 2 cut(s) 209, 464
AciI CCGC 6 cut(s) 9, 29, 275, 403, 464, 1163
AclWI GGATC 3 cut(s) 370, 431, 933
AcoI YGGCCR 1 cut(s) 933
AcuI CTGAAG 1 cut(s) 1321
AcyI GRCGYC 1 cut(s) 6
AfaI GTAC 7 cut(s) 410, 419, 623, 635, 679, 1152, 1227
AfiI CCNNNNNNNGG 2 cut(s) 942, 1341
AflIII ACRYGT 2 cut(s) 411, 505
AgsI TTSAA 2 cut(s) 154, 1358
AhdI GACNNNNNGTC 1 cut(s) 808
AjiI CACGTC 2 cut(s) 508, 1327
AjnI CCWGG 4 cut(s) 187, 744, 941, 1221
AleI CACNNNNGTG 1 cut(s) 789
AloI GAACNNNNNNTCC 2 cut(s) 434, 466
AluBI AGCT 3 cut(s) 351, 450, 1012
AluI AGCT 3 cut(s) 351, 450, 1012
Alw26I GTCTC 2 cut(s) 726, 1115
AlwI GGATC 3 cut(s) 370, 431, 933
AlwNI CAGNNNCTG 1 cut(s) 262
Ama87I CYCGRG 2 cut(s) 230, 236
AoxI GGCC 5 cut(s) 30, 457, 762, 913, 933
ApeKI GCWGC 2 cut(s) 26, 867
Asp700I GAANNNNTTC 1 cut(s) 380
Asp718I GGTACC 1 cut(s) 417
AspLEI GCGC 1 cut(s) 629
AspS9I GGNCC 3 cut(s) 762, 810, 989
AsuC2I CCSGG 1 cut(s) 729
AsuHPI GGTGA 4 cut(s) 385, 533, 973, 1030
AvaI CYCGRG 2 cut(s) 230, 236
AvaII GGWCC 2 cut(s) 810, 989
BaeI ACNNNNGTAYC 2 cut(s) 409, 442
BalI TGGCCA 1 cut(s) 935
BanI GGYRCC 2 cut(s) 417, 791
BanII GRGCYC 1 cut(s) 237
BauI CACGAG 1 cut(s) 340
BbvI GCAGC 2 cut(s) 38, 879
BciT130I CCWGG 4 cut(s) 189, 746, 943, 1223
BciVI GTATCC 1 cut(s) 647
BcnI CCSGG 1 cut(s) 729
BcoDI GTCTC 2 cut(s) 726, 1115
BfaI CTAG 2 cut(s) 314, 471
BfuI GTATCC 1 cut(s) 647
BglII AGATCT 1 cut(s) 131
BisI GCNGC 4 cut(s) 9, 27, 30, 868
BlsI GCNGC 4 cut(s) 10, 28, 31, 869
Bme1390I CCNGG 5 cut(s) 189, 729, 746, 943, 1223
Bme18I GGWCC 2 cut(s) 810, 989
BmeRI GACNNNNNGTC 1 cut(s) 808
BmeT110I CYCGRG 2 cut(s) 230, 236
BmgBI CACGTC 2 cut(s) 508, 1327
BmgT120I GGNCC 3 cut(s) 762, 810, 989
BmiI GGNNCC 4 cut(s) 419, 499, 793, 811
BmrFI CCNGG 5 cut(s) 189, 729, 746, 943, 1223
BmrI ACTGGG 2 cut(s) 769, 1302
BmsI GCATC 4 cut(s) 164, 268, 892, 1177
BmuI ACTGGG 2 cut(s) 769, 1302
BpuEI CTTGAG 2 cut(s) 701, 834
BpuMI CCSGG 1 cut(s) 729
BsaBI GATNNNNATC 2 cut(s) 602, 1103
BsaHI GRCGYC 1 cut(s) 6
BsaI GGTCTC 1 cut(s) 1115
BsaJI CCNNGG 4 cut(s) 229, 369, 421, 942
BsaWI WCCGGW 3 cut(s) 650, 688, 703
BsaXI ACNNNNNCTCC 4 cut(s) 256, 286, 434, 464
Bsc4I CCNNNNNNNGG 2 cut(s) 942, 1341
Bse1I ACTGG 4 cut(s) 641, 738, 764, 1297
Bse3DI GCAATG 2 cut(s) 92, 837
Bse8I GATNNNNATC 2 cut(s) 602, 1103
BseBI CCWGG 4 cut(s) 189, 746, 943, 1223
BseDI CCNNGG 4 cut(s) 229, 369, 421, 942
BseGI GGATG 2 cut(s) 936, 1308
BseJI GATNNNNATC 2 cut(s) 602, 1103
BseLI CCNNNNNNNGG 2 cut(s) 942, 1341
BseMI GCAATG 2 cut(s) 92, 837
BseNI ACTGG 4 cut(s) 641, 738, 764, 1297
BseRI GAGGAG 1 cut(s) 510
BseXI GCAGC 2 cut(s) 38, 879
BseYI CCCAGC 1 cut(s) 250
Bsh1236I CGCG 2 cut(s) 209, 464
BshFI GGCC 5 cut(s) 32, 459, 764, 915, 935
BshNI GGYRCC 2 cut(s) 417, 791
BsiHKCI CYCGRG 2 cut(s) 230, 236
BsiSI CCGG 4 cut(s) 651, 689, 704, 728
BslFI GGGAC 5 cut(s) 211, 359, 744, 959, 1208
BslI CCNNNNNNNGG 2 cut(s) 942, 1341
BsmAI GTCTC 2 cut(s) 726, 1115
BsmFI GGGAC 5 cut(s) 211, 359, 744, 959, 1208
BsmI GAATGC 1 cut(s) 1246
BsnI GGCC 5 cut(s) 32, 459, 764, 915, 935
Bso31I GGTCTC 1 cut(s) 1115
BsoBI CYCGRG 2 cut(s) 230, 236
Bsp1286I GDGCHC 1 cut(s) 237
Bsp1407I TGTACA 1 cut(s) 621
Bsp143I GATC 6 cut(s) 39, 131, 322, 362, 436, 938
BspACI CCGC 6 cut(s) 9, 29, 275, 403, 464, 1163
BspANI GGCC 5 cut(s) 32, 459, 764, 915, 935
BspFNI CGCG 2 cut(s) 209, 464
BspHI TCATGA 1 cut(s) 540
BspLI GGNNCC 4 cut(s) 419, 499, 793, 811
BspPI GGATC 3 cut(s) 370, 431, 933
BspQI GCTCTTC 1 cut(s) 393
BspT107I GGYRCC 2 cut(s) 417, 791
BspTNI GGTCTC 1 cut(s) 1115
BsrBI CCGCTC 2 cut(s) 403, 1163
BsrDI GCAATG 2 cut(s) 92, 837
BsrGI TGTACA 1 cut(s) 621
BsrI ACTGG 4 cut(s) 641, 738, 764, 1297
BssECI CCNNGG 4 cut(s) 229, 369, 421, 942
BssMI GATC 6 cut(s) 39, 131, 322, 362, 436, 938
BssNI GRCGYC 1 cut(s) 6
BssSI CACGAG 1 cut(s) 340
Bst2BI CACGAG 1 cut(s) 340
Bst2UI CCWGG 4 cut(s) 189, 746, 943, 1223
Bst4CI ACNGT 4 cut(s) 425, 659, 677, 788
Bst6I CTCTTC 1 cut(s) 393
BstACI GRCGYC 1 cut(s) 6
BstAUI TGTACA 1 cut(s) 621
BstC8I GCNNGC 1 cut(s) 1214
BstDEI CTNAG 2 cut(s) 576, 1169
BstDSI CCRYGG 2 cut(s) 369, 421
BstENI CCTNNNNNAGG 1 cut(s) 1339
BstF5I GGATG 2 cut(s) 936, 1308
BstFNI CGCG 2 cut(s) 209, 464
BstHHI GCGC 1 cut(s) 629
BstKTI GATC 6 cut(s) 42, 134, 325, 365, 439, 941
BstMAI GTCTC 2 cut(s) 726, 1115
BstMBI GATC 6 cut(s) 39, 131, 322, 362, 436, 938
BstMWI GCNNNNNNNGC 5 cut(s) 14, 23, 456, 497, 1218
BstNI CCWGG 4 cut(s) 189, 746, 943, 1223
BstNSI RCATGY 1 cut(s) 415
BstSCI CCNGG 5 cut(s) 187, 727, 744, 941, 1221
BstUI CGCG 2 cut(s) 209, 464
BstV1I GCAGC 2 cut(s) 38, 879
BstX2I RGATCY 3 cut(s) 131, 362, 938
BstXI CCANNNNNNTGG 1 cut(s) 931
BstYI RGATCY 3 cut(s) 131, 362, 938
BsuI GTATCC 1 cut(s) 647
BsuRI GGCC 5 cut(s) 32, 459, 764, 915, 935
BtgI CCRYGG 2 cut(s) 369, 421
BtgZI GCGATG 1 cut(s) 885
BtrI CACGTC 2 cut(s) 508, 1327
BtsCI GGATG 2 cut(s) 936, 1308
BtsI GCAGTG 1 cut(s) 636
BtsIMutI CAGTG 2 cut(s) 636, 793
Cac8I GCNNGC 1 cut(s) 1214
CaiI CAGNNNCTG 1 cut(s) 262
CciI TCATGA 1 cut(s) 540
CfoI GCGC 1 cut(s) 629
Cfr13I GGNCC 3 cut(s) 762, 810, 989
CseI GACGC 2 cut(s) 14, 719
Csp6I GTAC 7 cut(s) 409, 418, 622, 634, 678, 1151, 1226
CviAII CATG 8 cut(s) 300, 412, 511, 541, 607, 863, 877, 1191
CviQI GTAC 7 cut(s) 409, 418, 622, 634, 678, 1151, 1226
DdeI CTNAG 2 cut(s) 576, 1169
DpnI GATC 6 cut(s) 41, 133, 324, 364, 438, 940
DpnII GATC 6 cut(s) 39, 131, 322, 362, 436, 938
DriI GACNNNNNGTC 1 cut(s) 808
EaeI YGGCCR 1 cut(s) 933
Eam1104I CTCTTC 1 cut(s) 393
Eam1105I GACNNNNNGTC 1 cut(s) 808
EarI CTCTTC 1 cut(s) 393
EciI GGCGGA 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 237
Eco31I GGTCTC 1 cut(s) 1115
Eco47I GGWCC 2 cut(s) 810, 989
Eco57I CTGAAG 1 cut(s) 1321
Eco88I CYCGRG 2 cut(s) 230, 236
EcoNI CCTNNNNNAGG 1 cut(s) 1339
EcoRII CCWGG 4 cut(s) 187, 744, 941, 1221
EcoT22I ATGCAT 1 cut(s) 1192
EcoT38I GRGCYC 1 cut(s) 237
FaeI CATG 8 cut(s) 303, 415, 514, 544, 610, 866, 880, 1194
FaqI GGGAC 5 cut(s) 211, 359, 744, 959, 1208
FatI CATG 8 cut(s) 299, 411, 510, 540, 606, 862, 876, 1190
Fnu4HI GCNGC 4 cut(s) 9, 27, 30, 868
FokI GGATG 2 cut(s) 943, 1315
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 4 cut(s) 9, 27, 30, 868
FspBI CTAG 2 cut(s) 314, 471
GlaI GCGC 1 cut(s) 628
GluI GCNGC 4 cut(s) 9, 27, 30, 868
GsaI CCCAGC 1 cut(s) 254
HaeIII GGCC 5 cut(s) 32, 459, 764, 915, 935
HapII CCGG 4 cut(s) 651, 689, 704, 728
HgaI GACGC 2 cut(s) 14, 719
HhaI GCGC 1 cut(s) 629
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 8 cut(s) 303, 415, 514, 544, 610, 866, 880, 1194
Hin6I GCGC 1 cut(s) 627
HinP1I GCGC 1 cut(s) 627
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HinfI GANTC 7 cut(s) 192, 239, 481, 613, 768, 827, 1206
HpaII CCGG 4 cut(s) 651, 689, 704, 728
HphI GGTGA 4 cut(s) 385, 533, 973, 1030
Hpy166II GTNNAC 5 cut(s) 306, 634, 673, 709, 1143
Hpy188III TCNNGA 3 cut(s) 541, 1124, 1179
Hpy8I GTNNAC 5 cut(s) 306, 634, 673, 709, 1143
Hpy99I CGWCG 1 cut(s) 806
HpyCH4III ACNGT 4 cut(s) 425, 659, 677, 788
HpyCH4IV ACGT 3 cut(s) 507, 804, 1326
HpyCH4V TGCA 5 cut(s) 842, 862, 905, 1190, 1244
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 23, 456, 497, 1218
HpyF3I CTNAG 2 cut(s) 576, 1169
HpySE526I ACGT 3 cut(s) 507, 804, 1326
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 8 cut(s) 303, 415, 514, 544, 610, 866, 880, 1194
HspAI GCGC 1 cut(s) 627
KpnI GGTACC 1 cut(s) 421
Kzo9I GATC 6 cut(s) 39, 131, 322, 362, 436, 938
LguI GCTCTTC 1 cut(s) 393
LmnI GCTCC 4 cut(s) 356, 455, 497, 1160
Lsp1109I GCAGC 2 cut(s) 38, 879
LweI GCATC 4 cut(s) 164, 268, 892, 1177
MaeI CTAG 2 cut(s) 314, 471
MaeII ACGT 3 cut(s) 507, 804, 1326
MaeIII GTNAC 2 cut(s) 329, 1322
MalI GATC 6 cut(s) 41, 133, 324, 364, 438, 940
MbiI CCGCTC 2 cut(s) 403, 1163
MboI GATC 6 cut(s) 39, 131, 322, 362, 436, 938
MboII GAAGA 4 cut(s) 60, 410, 1108, 1292
MfeI CAATTG 1 cut(s) 556
MflI RGATCY 3 cut(s) 131, 362, 938
MhlI GDGCHC 1 cut(s) 237
MlsI TGGCCA 1 cut(s) 935
MluCI AATT 3 cut(s) 149, 556, 975
MluNI TGGCCA 1 cut(s) 935
MlyI GAGTC 3 cut(s) 248, 622, 1215
MmeI TCCRAC 1 cut(s) 1255
Mox20I TGGCCA 1 cut(s) 935
Mph1103I ATGCAT 1 cut(s) 1192
MroXI GAANNNNTTC 1 cut(s) 380
MscI TGGCCA 1 cut(s) 935
MseI TTAA 1 cut(s) 585
MslI CAYNNNNRTG 4 cut(s) 298, 789, 881, 929
Msp20I TGGCCA 1 cut(s) 935
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 4 cut(s) 651, 689, 704, 728
MspR9I CCNGG 5 cut(s) 189, 729, 746, 943, 1223
MunI CAATTG 1 cut(s) 556
Mva1269I GAATGC 1 cut(s) 1246
MvaI CCWGG 4 cut(s) 189, 746, 943, 1223
MvnI CGCG 2 cut(s) 209, 464
MwoI GCNNNNNNNGC 5 cut(s) 14, 23, 456, 497, 1218
NciI CCSGG 1 cut(s) 729
NdeII GATC 6 cut(s) 39, 131, 322, 362, 436, 938
NlaIII CATG 8 cut(s) 303, 415, 514, 544, 610, 866, 880, 1194
NlaIV GGNNCC 4 cut(s) 419, 499, 793, 811
NmuCI GTSAC 1 cut(s) 1322
NsiI ATGCAT 1 cut(s) 1192
NspI RCATGY 1 cut(s) 415
OliI CACNNNNGTG 1 cut(s) 789
PaeR7I CTCGAG 1 cut(s) 236
PagI TCATGA 1 cut(s) 540
PciI ACATGT 1 cut(s) 411
PciSI GCTCTTC 1 cut(s) 393
PcsI WCGNNNNNNNCGW 2 cut(s) 366, 437
PctI GAATGC 1 cut(s) 1246
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 4 cut(s) 192, 481, 768, 827
PflFI GACNNNGTC 1 cut(s) 301
PkrI GCNGC 4 cut(s) 10, 28, 31, 869
PleI GAGTC 3 cut(s) 247, 621, 1214
PpsI GAGTC 3 cut(s) 247, 621, 1214
PscI ACATGT 1 cut(s) 411
Psp6I CCWGG 4 cut(s) 187, 744, 941, 1221
PspFI CCCAGC 1 cut(s) 250
PspGI CCWGG 4 cut(s) 187, 744, 941, 1221
PspN4I GGNNCC 4 cut(s) 419, 499, 793, 811
PspPI GGNCC 3 cut(s) 762, 810, 989
PspXI VCTCGAGB 1 cut(s) 236
PstNI CAGNNNCTG 1 cut(s) 262
PsuI RGATCY 3 cut(s) 131, 362, 938
PsyI GACNNNGTC 1 cut(s) 301
RsaI GTAC 7 cut(s) 410, 419, 623, 635, 679, 1152, 1227
RsaNI GTAC 7 cut(s) 409, 418, 622, 634, 678, 1151, 1226
RseI CAYNNNNRTG 4 cut(s) 298, 789, 881, 929
SapI GCTCTTC 1 cut(s) 393
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 4 cut(s) 9, 27, 30, 868
Sau3AI GATC 6 cut(s) 39, 131, 322, 362, 436, 938
Sau96I GGNCC 3 cut(s) 762, 810, 989
SchI GAGTC 3 cut(s) 248, 622, 1215
ScrFI CCNGG 5 cut(s) 189, 729, 746, 943, 1223
SduI GDGCHC 1 cut(s) 237
SfaNI GCATC 4 cut(s) 164, 268, 892, 1177
Sfr274I CTCGAG 1 cut(s) 236
SinI GGWCC 2 cut(s) 810, 989
SlaI CTCGAG 1 cut(s) 236
SmiMI CAYNNNNRTG 4 cut(s) 298, 789, 881, 929
SmlI CTYRAG 3 cut(s) 236, 716, 849
SmoI CTYRAG 3 cut(s) 236, 716, 849
Sse9I AATT 3 cut(s) 149, 556, 975
SsiI CCGC 6 cut(s) 9, 29, 275, 403, 464, 1163
SspMI CTAG 2 cut(s) 314, 471
StyD4I CCNGG 5 cut(s) 187, 727, 744, 941, 1221
TaaI ACNGT 4 cut(s) 425, 659, 677, 788
TaiI ACGT 3 cut(s) 510, 807, 1329
TaqI TCGA 7 cut(s) 195, 237, 296, 551, 611, 771, 801
TaqII GACCGA 1 cut(s) 1097
TasI AATT 3 cut(s) 149, 556, 975
TatI WGTACW 3 cut(s) 621, 633, 677
TauI GCSGC 2 cut(s) 11, 32
TfiI GAWTC 4 cut(s) 192, 481, 768, 827
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 2 cut(s) 636, 793
TseFI GTSAC 1 cut(s) 1322
TseI GCWGC 2 cut(s) 26, 867
Tsp45I GTSAC 1 cut(s) 1322
TspDTI ATGAA 5 cut(s) 153, 373, 411, 473, 557
TspGWI ACGGA 2 cut(s) 963, 1173
TspRI CASTG 2 cut(s) 636, 793
Tth111I GACNNNGTC 1 cut(s) 301
VpaK11BI GGWCC 2 cut(s) 810, 989
XagI CCTNNNNNAGG 1 cut(s) 1339
XceI RCATGY 1 cut(s) 415
XcmI CCANNNNNNNNNTGG 1 cut(s) 923
XhoI CTCGAG 1 cut(s) 236
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 2 cut(s) 314, 471
Zsp2I ATGCAT 1 cut(s) 1192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.