RLG00000006276

methyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0018029
Physical Location & Seq
Reverse (-)
5838084 .. 5839460
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006276

Sequence Viewer

Length: 1377 bp
ATGGACGCAATAACAGCAACAGCAGCAGCCTCAACGATCAAAACGATTCACTCATTTACGCCTTCTTCTACAACCCCTAAAATTGTGGTATTGCCTACTATCGGACCCCAAACACTCTCTCTTTACACCGCCAACAGATCTTTCCACACCCAACAATTTAGAAGTCGTTCATTTACAAGATGCTCTACAAAGCCAGGAATCGACAGCAACAACGCAACCAACACAAACAAGTCCATCGGGCTGGAGTCGAGTTCCCCAGCACAACATCTGACGACTTCTCCGCCCAACCAAACTCTCACATCTACATGGTCAACGGGTCTGGTCTTTGATCTGGGTCAGAAAACCTCGTGGGACAGTTCCGAAATCGGATCTCCCGTGGTCAAAAGGTTCATCGGCGACAATGAAGAGCGGTGGTACATGTGGTACCACGGTAGGTCGTCCGATCCGAACAACAGTTCCGACTCCATCGGCCTCGCGGTTTCGAGTAATGGGATTCATTGGGCGAGAGGAGGCGAACACGTCATGTCTTGTGGTGAGGACGAGGGTTTGGTCATGAGCTGCTCGGACAATTGGTGGGCGTTCGACACAAAGAGCATTATGCCTTCCGAGATGGTCATCATGTCGAGTCCAATGTACAGTGCAGTGTACTGGCTTTACTACACCGGGTACAGTTCCGAAAAGGTGGACTGTCGTAACTATCCGGTTGGTCAATGTTCAGTGGACTCGTTTAAGTCTCTGCCCGGACTGGCTTGCAGCCAGGACGGGCGAAACTGGGCCAGGATTGAAGGGGATTATCATAGTGGTGCCTTGCTCGACGTAGGGTCCAAAAATGAGTGGGATTCTTTGTTCATTGCAAAACCTCAAGTGGTTGTGCACGGCGGCGACGACATGAGAATGTATTATCATTCGTTTGATGCAGAAAACGGTCACTTTGGCATTGGAATGGCGAGATCCAGGGACGGAATAAGGTGGGCGAAGTTGGGGAAAATTATGGGGGGAGGGGCAAAAGGGTCATTTGACGAGCTTGGGGTGAAGAATGCTCGTGTGGTTAGGAACAGGAAGGAAGGTAACTACTTAATGGCGTATGAGGGTGTGAGCGCGGATGGAAAGATGAGTATTGGTCTGGCTGTATCACCCGATGGGCTGAAGGACTGGACCAGAGTCCAAGACTGGTCAGTTCTCAGCCCATCAGAAGAAGACGGATGGGACAACAGAGGAGTGGGATCCCCGAGTCTGGTGCATATGGAAGGGAATGTAGACATGTGGAGATTGTATTATGTGGGTGTTGGAGAAGATGGAAGGACTGGGATTGGGATGGCGATTTCGGAAGGTAGTAACGTCAGAAACTTCAGACGGTGGGCAGGGTTTCAATCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

459

Amino Acids

50.3

Weight (kDa)

6.02

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018029)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g30990
rosa_chinensis RchiOBHm_Chr4g0439361
rosa_laevigata RLG00000006276
rosa_multiflora Rmu_co8431667.1_g000001 Rmu_sc0004012.1_g000003
rosa_roxburghii Rroxscaffold_5G00380290
rosa_samantha Rh4AG368800 Rh4BG380600 Rh4CG395100 Rh4DG375400
rosa_wichuraiana Rw4G031450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 2 cut(s) 423, 803
AccBSI CCGCTC 1 cut(s) 409
AccI GTMKAC 1 cut(s) 1257
AccII CGCG 2 cut(s) 476, 1100
AciI CCGC 6 cut(s) 129, 281, 409, 476, 879, 1100
AclWI GGATC 5 cut(s) 376, 437, 945, 1218, 1231
AcuI CTGAAG 2 cut(s) 1166, 1333
AfaI GTAC 5 cut(s) 416, 425, 635, 647, 668
AfiI CCNNNNNNNGG 2 cut(s) 101, 1234
AflIII ACRYGT 3 cut(s) 417, 517, 1260
AgsI TTSAA 2 cut(s) 785, 1370
AhdI GACNNNNNGTC 1 cut(s) 820
AjiI CACGTC 1 cut(s) 520
AjnI CCWGG 4 cut(s) 193, 756, 776, 953
AjuI GAANNNNNNNTTGG 2 cut(s) 125, 157
AloI GAACNNNNNNTCC 4 cut(s) 440, 472, 830, 862
AluBI AGCT 2 cut(s) 558, 1024
AluI AGCT 2 cut(s) 558, 1024
Alw21I GWGCWC 1 cut(s) 876
Alw26I GTCTC 1 cut(s) 738
Alw44I GTGCAC 1 cut(s) 872
AlwI GGATC 5 cut(s) 376, 437, 945, 1218, 1231
Ama87I CYCGRG 1 cut(s) 1228
AoxI GGCC 2 cut(s) 469, 774
ApaLI GTGCAC 1 cut(s) 872
ApeKI GCWGC 4 cut(s) 23, 26, 558, 753
Asp700I GAANNNNTTC 1 cut(s) 166
Asp718I GGTACC 1 cut(s) 423
AspLEI GCGC 1 cut(s) 1100
AspS9I GGNCC 4 cut(s) 104, 774, 822, 1155
AsuC2I CCSGG 2 cut(s) 664, 741
AsuHPI GGTGA 3 cut(s) 545, 1042, 1125
AvaI CYCGRG 1 cut(s) 1228
AvaII GGWCC 3 cut(s) 104, 822, 1155
BaeGI GKGCMC 1 cut(s) 876
BaeI ACNNNNGTAYC 2 cut(s) 415, 448
BamHI GGATCC 1 cut(s) 1223
BanI GGYRCC 2 cut(s) 423, 803
BauI CACGAG 2 cut(s) 346, 1041
BbsI GAAGAC 1 cut(s) 1203
Bbv12I GWGCWC 1 cut(s) 876
BbvI GCAGC 4 cut(s) 35, 38, 545, 765
BccI CCATC 9 cut(s) 242, 473, 604, 1097, 1133, 1195, 1197, 1289, 1309
BceAI ACGGC 1 cut(s) 892
BcgI CGANNNNNNTGC 2 cut(s) 1219, 1253
BciT130I CCWGG 4 cut(s) 195, 758, 778, 955
BcnI CCSGG 2 cut(s) 664, 741
BcoDI GTCTC 1 cut(s) 738
BglII AGATCT 1 cut(s) 137
BisI GCNGC 5 cut(s) 24, 27, 559, 754, 880
BlsI GCNGC 5 cut(s) 25, 28, 560, 755, 881
Bme1390I CCNGG 6 cut(s) 195, 664, 741, 758, 778, 955
Bme18I GGWCC 3 cut(s) 104, 822, 1155
BmeRI GACNNNNNGTC 1 cut(s) 820
BmeT110I CYCGRG 1 cut(s) 1228
BmgBI CACGTC 1 cut(s) 520
BmgT120I GGNCC 4 cut(s) 104, 774, 822, 1155
BmiI GGNNCC 5 cut(s) 106, 425, 805, 823, 1225
BmrFI CCNGG 6 cut(s) 195, 664, 741, 758, 778, 955
BmrI ACTGGG 2 cut(s) 781, 1314
BmsI GCATC 2 cut(s) 170, 904
BmuI ACTGGG 2 cut(s) 781, 1314
BoxI GACNNNNGTC 1 cut(s) 1160
BpiI GAAGAC 1 cut(s) 1203
BpmI CTGGAG 1 cut(s) 263
BpuEI CTTGAG 1 cut(s) 846
BpuMI CCSGG 2 cut(s) 664, 741
BsaBI GATNNNNATC 1 cut(s) 614
BsaJI CCNNGG 3 cut(s) 375, 427, 954
BsaWI WCCGGW 1 cut(s) 700
BsaXI ACNNNNNCTCC 2 cut(s) 262, 292
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 1234
Bse1I ACTGG 6 cut(s) 653, 750, 776, 1157, 1175, 1309
Bse3DI GCAATG 1 cut(s) 849
Bse8I GATNNNNATC 1 cut(s) 614
BseBI CCWGG 4 cut(s) 195, 758, 778, 955
BseDI CCNNGG 3 cut(s) 375, 427, 954
BseGI GGATG 3 cut(s) 1108, 1208, 1320
BseJI GATNNNNATC 1 cut(s) 614
BseLI CCNNNNNNNGG 2 cut(s) 101, 1234
BseMI GCAATG 1 cut(s) 849
BseMII CTCAG 1 cut(s) 1195
BseNI ACTGG 6 cut(s) 653, 750, 776, 1157, 1175, 1309
BseRI GAGGAG 2 cut(s) 522, 1230
BseSI GKGCMC 1 cut(s) 876
BseXI GCAGC 4 cut(s) 35, 38, 545, 765
BseYI CCCAGC 1 cut(s) 256
BsgI GTGCAG 1 cut(s) 660
Bsh1236I CGCG 2 cut(s) 476, 1100
BshFI GGCC 2 cut(s) 471, 776
BshNI GGYRCC 2 cut(s) 423, 803
BsiHKAI GWGCWC 1 cut(s) 876
BsiHKCI CYCGRG 1 cut(s) 1228
BsiSI CCGG 3 cut(s) 663, 701, 741
BslFI GGGAC 3 cut(s) 365, 971, 1220
BslI CCNNNNNNNGG 2 cut(s) 101, 1234
BsmAI GTCTC 1 cut(s) 738
BsmFI GGGAC 3 cut(s) 365, 971, 1220
BsmI GAATGC 1 cut(s) 1042
BsnI GGCC 2 cut(s) 471, 776
BsoBI CYCGRG 1 cut(s) 1228
Bsp1286I GDGCHC 1 cut(s) 876
Bsp1407I TGTACA 1 cut(s) 633
Bsp143I GATC 7 cut(s) 36, 137, 328, 368, 442, 950, 1223
BspACI CCGC 6 cut(s) 129, 281, 409, 476, 879, 1100
BspANI GGCC 2 cut(s) 471, 776
BspCNI CTCAG 1 cut(s) 1194
BspFNI CGCG 2 cut(s) 476, 1100
BspHI TCATGA 1 cut(s) 552
BspLI GGNNCC 5 cut(s) 106, 425, 805, 823, 1225
BspPI GGATC 5 cut(s) 376, 437, 945, 1218, 1231
BspQI GCTCTTC 1 cut(s) 399
BspT107I GGYRCC 2 cut(s) 423, 803
BsrBI CCGCTC 1 cut(s) 409
BsrDI GCAATG 1 cut(s) 849
BsrGI TGTACA 1 cut(s) 633
BsrI ACTGG 6 cut(s) 653, 750, 776, 1157, 1175, 1309
BssECI CCNNGG 3 cut(s) 375, 427, 954
BssMI GATC 7 cut(s) 36, 137, 328, 368, 442, 950, 1223
BssSI CACGAG 2 cut(s) 346, 1041
Bst2BI CACGAG 2 cut(s) 346, 1041
Bst2UI CCWGG 4 cut(s) 195, 758, 778, 955
Bst4CI ACNGT 8 cut(s) 356, 431, 455, 638, 671, 689, 926, 1356
Bst6I CTCTTC 1 cut(s) 399
BstAUI TGTACA 1 cut(s) 633
BstC8I GCNNGC 1 cut(s) 751
BstDEI CTNAG 1 cut(s) 1181
BstDSI CCRYGG 2 cut(s) 375, 427
BstF5I GGATG 3 cut(s) 1108, 1208, 1320
BstFNI CGCG 2 cut(s) 476, 1100
BstHHI GCGC 1 cut(s) 1100
BstKTI GATC 7 cut(s) 39, 140, 331, 371, 445, 953, 1226
BstMAI GTCTC 1 cut(s) 738
BstMBI GATC 7 cut(s) 36, 137, 328, 368, 442, 950, 1223
BstMWI GCNNNNNNNGC 2 cut(s) 14, 23
BstNI CCWGG 4 cut(s) 195, 758, 778, 955
BstNSI RCATGY 2 cut(s) 421, 1264
BstPAI GACNNNNGTC 1 cut(s) 1160
BstSCI CCNGG 6 cut(s) 193, 662, 739, 756, 776, 953
BstSLI GKGCMC 1 cut(s) 876
BstUI CGCG 2 cut(s) 476, 1100
BstV1I GCAGC 4 cut(s) 35, 38, 545, 765
BstV2I GAAGAC 1 cut(s) 1203
BstX2I RGATCY 4 cut(s) 137, 368, 950, 1223
BstXI CCANNNNNNTGG 1 cut(s) 241
BstYI RGATCY 4 cut(s) 137, 368, 950, 1223
BsuRI GGCC 2 cut(s) 471, 776
BtgI CCRYGG 2 cut(s) 375, 427
BtrI CACGTC 1 cut(s) 520
BtsCI GGATG 3 cut(s) 1108, 1208, 1320
BtsI GCAGTG 1 cut(s) 648
BtsIMutI CAGTG 3 cut(s) 643, 648, 723
Cac8I GCNNGC 1 cut(s) 751
CciI TCATGA 1 cut(s) 552
CfoI GCGC 1 cut(s) 1100
Cfr13I GGNCC 4 cut(s) 104, 774, 822, 1155
CseI GACGC 1 cut(s) 14
Csp6I GTAC 5 cut(s) 415, 424, 634, 646, 667
CviAII CATG 7 cut(s) 306, 418, 523, 553, 619, 889, 1261
CviQI GTAC 5 cut(s) 415, 424, 634, 646, 667
DdeI CTNAG 1 cut(s) 1181
DpnI GATC 7 cut(s) 38, 139, 330, 370, 444, 952, 1225
DpnII GATC 7 cut(s) 36, 137, 328, 368, 442, 950, 1223
DriI GACNNNNNGTC 1 cut(s) 820
Eam1104I CTCTTC 1 cut(s) 399
Eam1105I GACNNNNNGTC 1 cut(s) 820
EarI CTCTTC 1 cut(s) 399
EciI GGCGGA 1 cut(s) 270
Eco47I GGWCC 3 cut(s) 104, 822, 1155
Eco57I CTGAAG 2 cut(s) 1166, 1333
Eco88I CYCGRG 1 cut(s) 1228
EcoRII CCWGG 4 cut(s) 193, 756, 776, 953
FaeI CATG 7 cut(s) 309, 421, 526, 556, 622, 892, 1264
FaqI GGGAC 3 cut(s) 365, 971, 1220
FatI CATG 7 cut(s) 305, 417, 522, 552, 618, 888, 1260
FauNDI CATATG 1 cut(s) 1242
FblI GTMKAC 1 cut(s) 1257
Fnu4HI GCNGC 5 cut(s) 24, 27, 559, 754, 880
FokI GGATG 3 cut(s) 1115, 1215, 1327
Fsp4HI GCNGC 5 cut(s) 24, 27, 559, 754, 880
GlaI GCGC 1 cut(s) 1099
GluI GCNGC 5 cut(s) 24, 27, 559, 754, 880
GsaI CCCAGC 1 cut(s) 260
GsuI CTGGAG 1 cut(s) 263
HaeIII GGCC 2 cut(s) 471, 776
HapII CCGG 3 cut(s) 663, 701, 741
HgaI GACGC 1 cut(s) 14
HhaI GCGC 1 cut(s) 1100
Hin1II CATG 7 cut(s) 309, 421, 526, 556, 622, 892, 1264
Hin6I GCGC 1 cut(s) 1098
HinP1I GCGC 1 cut(s) 1098
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HpaII CCGG 3 cut(s) 663, 701, 741
HphI GGTGA 3 cut(s) 545, 1042, 1125
Hpy166II GTNNAC 6 cut(s) 312, 646, 685, 721, 874, 1258
Hpy188III TCNNGA 1 cut(s) 553
Hpy8I GTNNAC 6 cut(s) 312, 646, 685, 721, 874, 1258
Hpy99I CGWCG 2 cut(s) 818, 887
HpyAV CCTTC 9 cut(s) 72, 612, 779, 1054, 1058, 1141, 1241, 1293, 1322
HpyCH4III ACNGT 8 cut(s) 356, 431, 455, 638, 671, 689, 926, 1356
HpyCH4IV ACGT 3 cut(s) 519, 816, 1338
HpyCH4V TGCA 6 cut(s) 641, 753, 854, 874, 917, 1240
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 23
HpyF3I CTNAG 1 cut(s) 1181
HpySE526I ACGT 3 cut(s) 519, 816, 1338
Hsp92II CATG 7 cut(s) 309, 421, 526, 556, 622, 892, 1264
HspAI GCGC 1 cut(s) 1098
KpnI GGTACC 1 cut(s) 427
Kzo9I GATC 7 cut(s) 36, 137, 328, 368, 442, 950, 1223
LguI GCTCTTC 1 cut(s) 399
Lsp1109I GCAGC 4 cut(s) 35, 38, 545, 765
LweI GCATC 2 cut(s) 170, 904
MaeII ACGT 3 cut(s) 519, 816, 1338
MaeIII GTNAC 4 cut(s) 692, 926, 1067, 1334
MalI GATC 7 cut(s) 38, 139, 330, 370, 444, 952, 1225
MbiI CCGCTC 1 cut(s) 409
MboI GATC 7 cut(s) 36, 137, 328, 368, 442, 950, 1223
MboII GAAGA 6 cut(s) 57, 416, 1045, 1205, 1208, 1304
MfeI CAATTG 1 cut(s) 568
MflI RGATCY 4 cut(s) 137, 368, 950, 1223
MhlI GDGCHC 1 cut(s) 876
MluCI AATT 4 cut(s) 81, 155, 568, 987
MlyI GAGTC 6 cut(s) 254, 455, 634, 716, 1170, 1240
MmeI TCCRAC 2 cut(s) 483, 1267
MroXI GAANNNNTTC 1 cut(s) 166
MseI TTAA 2 cut(s) 729, 1076
MslI CAYNNNNRTG 4 cut(s) 304, 801, 893, 941
MspI CCGG 3 cut(s) 663, 701, 741
MspR9I CCNGG 6 cut(s) 195, 664, 741, 758, 778, 955
MunI CAATTG 1 cut(s) 568
Mva1269I GAATGC 1 cut(s) 1042
MvaI CCWGG 4 cut(s) 195, 758, 778, 955
MvnI CGCG 2 cut(s) 476, 1100
MwoI GCNNNNNNNGC 2 cut(s) 14, 23
NciI CCSGG 2 cut(s) 664, 741
NdeI CATATG 1 cut(s) 1242
NdeII GATC 7 cut(s) 36, 137, 328, 368, 442, 950, 1223
NlaIII CATG 7 cut(s) 309, 421, 526, 556, 622, 892, 1264
NlaIV GGNNCC 5 cut(s) 106, 425, 805, 823, 1225
NmuCI GTSAC 1 cut(s) 926
NspI RCATGY 2 cut(s) 421, 1264
PagI TCATGA 1 cut(s) 552
PciI ACATGT 2 cut(s) 417, 1260
PciSI GCTCTTC 1 cut(s) 399
PcsI WCGNNNNNNNCGW 4 cut(s) 41, 372, 443, 882
PctI GAATGC 1 cut(s) 1042
PdmI GAANNNNTTC 1 cut(s) 166
PfeI GAWTC 4 cut(s) 46, 198, 493, 839
PkrI GCNGC 5 cut(s) 25, 28, 560, 755, 881
PleI GAGTC 6 cut(s) 253, 455, 633, 716, 1169, 1239
PpsI GAGTC 6 cut(s) 253, 455, 633, 716, 1169, 1239
PscI ACATGT 2 cut(s) 417, 1260
PshAI GACNNNNGTC 1 cut(s) 1160
Psp6I CCWGG 4 cut(s) 193, 756, 776, 953
PspFI CCCAGC 1 cut(s) 256
PspGI CCWGG 4 cut(s) 193, 756, 776, 953
PspN4I GGNNCC 5 cut(s) 106, 425, 805, 823, 1225
PspPI GGNCC 4 cut(s) 104, 774, 822, 1155
PsuI RGATCY 4 cut(s) 137, 368, 950, 1223
RsaI GTAC 5 cut(s) 416, 425, 635, 647, 668
RsaNI GTAC 5 cut(s) 415, 424, 634, 646, 667
RseI CAYNNNNRTG 4 cut(s) 304, 801, 893, 941
SapI GCTCTTC 1 cut(s) 399
SaqAI TTAA 2 cut(s) 729, 1076
SatI GCNGC 5 cut(s) 24, 27, 559, 754, 880
Sau3AI GATC 7 cut(s) 36, 137, 328, 368, 442, 950, 1223
Sau96I GGNCC 4 cut(s) 104, 774, 822, 1155
SchI GAGTC 6 cut(s) 254, 455, 634, 716, 1170, 1240
ScrFI CCNGG 6 cut(s) 195, 664, 741, 758, 778, 955
SduI GDGCHC 1 cut(s) 876
SfaNI GCATC 2 cut(s) 170, 904
SinI GGWCC 3 cut(s) 104, 822, 1155
SmiMI CAYNNNNRTG 4 cut(s) 304, 801, 893, 941
SmlI CTYRAG 1 cut(s) 861
SmoI CTYRAG 1 cut(s) 861
Sse9I AATT 4 cut(s) 81, 155, 568, 987
SsiI CCGC 6 cut(s) 129, 281, 409, 476, 879, 1100
StyD4I CCNGG 6 cut(s) 193, 662, 739, 756, 776, 953
TaaI ACNGT 8 cut(s) 356, 431, 455, 638, 671, 689, 926, 1356
TaiI ACGT 3 cut(s) 522, 819, 1341
TaqI TCGA 6 cut(s) 201, 248, 482, 582, 623, 813
TasI AATT 4 cut(s) 81, 155, 568, 987
TatI WGTACW 2 cut(s) 633, 645
TauI GCSGC 1 cut(s) 882
TfiI GAWTC 4 cut(s) 46, 198, 493, 839
Tru1I TTAA 2 cut(s) 729, 1076
Tru9I TTAA 2 cut(s) 729, 1076
TscAI CASTG 3 cut(s) 643, 648, 723
TseFI GTSAC 1 cut(s) 926
TseI GCWGC 4 cut(s) 23, 26, 558, 753
Tsp45I GTSAC 1 cut(s) 926
TspDTI ATGAA 5 cut(s) 159, 379, 417, 485, 838
TspGWI ACGGA 2 cut(s) 975, 1215
TspRI CASTG 3 cut(s) 643, 648, 723
VneI GTGCAC 1 cut(s) 872
VpaK11BI GGWCC 3 cut(s) 104, 822, 1155
XceI RCATGY 2 cut(s) 421, 1264
XmiI GTMKAC 1 cut(s) 1257
XmnI GAANNNNTTC 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.