Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0006443

Physical Location & Seq

Genomic Coordinates Forward (+)

31612619 .. 31626912

Gene Structure 14294 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g33380.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2487 bp

ATGCCACGCATCATGACTGTTGCTGGCTTGATATCTGATGTTGATGATGACAAGCCGGAGAGTGTGTGCTCCGAGGTGTCGTCGTCTTCGGTCCAGCGTGACCGGATCATTATAGTGGCTAATCAGCTACCCATTAGAGCTCAGAGAAAGTCTGATACTAGTAAGGGTTGGATTTTCAGTTGGGATGAGAATTCGTTGCTGCTTCAGCTGAAAGATGGATTAGGGGATGATGAGATTGAGGTTATTTATGTGGGGTGTCTTAAGGAGGAGATTCACCCCAATGAGCAGGAGGAGGTTTCCCAAATCCTGCTCGAGACATTTAAATGTGTCCCGACGTTTCTCCCACCGGAGCTTTTTAGCCGGTACTATCATGGTTTTTGTAAGCAGCAGTTGTGGCCATTGTTTCATTACATGCTGCCGCTGTCTCCGGACCATGGTGGGAGGTTCAACCGGTCGTTGTGGCAAGCATATGTGTCTGTGAACAAGATTTTCGCGGATAGGATCATGGAGGTCATTAACCCCGAAGATGATTTTGTGTGGATACATGATTATCATCTGATGGTGTTGCCAACTTTCTTGCGGAAGAGATTCAACCGTGTGAAACTTGGGTTTTTCCTGCATAGCCCTTTCCCTTCGTCTGAAATTTACAAGACATTGCCTATTAGGGAAGAGATTCTACGAGCCCTTTTGAATTCTGATTTGATTGGATTCCATACATTTGATTATGCAAGGCATTTCCTCTCTTGTTGTAGTCGAATGCTTGGTCTTAGCTATGAATCCAAGCGAGGTTATATAGGCCTCGAGTATTATGGTCGGACTGTTAGCATTAAAATTCTTCCGGTAGGCATACATATGGGTCAACTGCAGTCAGTTTTGAGCCTCCCAGAGACAGAAGCCAAGGTTGCTGAGCTCAAGGCGCAGTTCTGTGATCGAGGTAGGATAATGTTACTTGGAGTAGATGACATGGATATTTTTAAGGGCATAAGTTTGAAGCTTTTGGCAATGGAGCAGCTGCTTATTCAGCACCCTGAGTGGCAGGGAAGGGTTGTATTGGTGCAGATAGCCAATCCAGCAAGGGGTCGGGGAAAAGATGTGAAAGAAGTTCAGGCGGAGACATCGTCAACTGTGAAGCGAATTAATGAAACATTTGGCAAACCTGGGTATAAGCCTGTTTTGTTAATTGATGAACCACTCAAGTTTTATGAGAGAATTGCATACTATGTAGTTGCAGAGTGTTGTTTGGTTACGGCTGTCAGGGATGGAATGAATTTGATACCATATGAATACATTATTAGTCGTCAAGGAAATGAAAAATTGGATAAGGTTTTGGGATTGGATTCCTCTGTTCCCAAGAAGAGTATGCTAGTTGTCTCAGAGTTCATTGGCTGCTCCCCTTCTTTGAGTGGAGCAATTCGTGTGAACCCCTGGAATATTGATGCTGTGGCAGATGCAATGGACTGTTCTCTGGAGATGGCGGAGCCAGAAACACAGCTCCGGCATGAGAAGCATTATAAATATGTCAGCACCCATGATGTTGGTTACTGGGCTCGCAGTTTCCTCCAAGACTTGGAAAGGACATGCAAAGGTCATTTGCGTCAAAGGTGCTGGGGTATTGGGTTTGGGTTGAGTTTTAGAGTTGTGGCTCTTGATCCAAACTTCAAGAAGCTCTCTGTGGAATACATTGTGTCAGCCTACAAAAGGACTACAACTAGGGCAATACTTCTCGACTATGATGGCACATTGAAGCCTCAGGCATCCATTGATAAGAGTCCAAATTCTAAATCCATTGAAATCCTAAATAGCCTATGCAGGGACAAGAACAACATGGTTTTGGTTGTTAGTGCTAAGAGTCGAAAAACTCTTTCTGAGTGGTTCTCTCCTTGTGAGAAGCTTGGACTTGCAGCTGAGCATGGCTACTTCCTACGGTCGAAGCAAAATACGGAATGGGAAACATTGGTACCAATTGCAGACAGTAGCTGGAAGCAGATTGCAGAGCCAGTCATGAAACTTTACACAGAAACAACTGATGGTTCCACCATTGAAGATAAGGAAACTTCACTTCTCTGGAGTTATGAGGATGCAGATCCAGACTTTGGATCATGCCAAGCCAAGGAACTTATGGATCATCTTGAAAGTGTGCTCGCTAATGAACCGGTTACTGTTAAAAGTGGGCAGACATTTGTGGAGGTTAAACCACAGGGTGTAAGCAAGGGACTGGTAGCCAAACGTCTACTTTCCACTATGCAAGAAAAGGGAATGATACCAGACTTTGTTCTCTGCATAGGGGATGACCGATCTGATGAAGACATGTTTGAGGTAATTACAAGTTCCATGGAAGGCCCATCAATTGCTCCCAGAGCAGAAGTATTCGCGTGTACAATTTGTCAAAAACCCAGCAAAGCCAAGTATTATCTGGATGACACGGCGGAGATTGTGAGGATGCTGCAGGGTTTGGCATCTGTTTCTGAGCAAAACATACCTCTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003825 GO:0004805 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016311 GO:0016740 GO:0016757 GO:0016758 GO:0016787 GO:0016788 GO:0016791 GO:0019203 GO:0033554 GO:0034637 GO:0035251 GO:0042578 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044464 GO:0046351 GO:0046527 GO:0050896 GO:0051716 GO:0070413 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

829

Amino Acids

93.93

Weight (kDa)

5.72

Isoelectric Point (pI)

42.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	62 - 551	2.9e-185	Glycosyltransferase family 20
Trehalose_PPase	PF02358	601 - 835	1.1e-78	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006443)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68020 AT1G68020 AT1G68020
fragaria_vesca	FvH4_4g33380 FvH4_4g33380 FvH4_4g33380 FvH4_4g33380 FvH4_4g33380 FvH4_4g33380 FvH4_4g33380 FvH4_4g33380
malus_domestica	MD16G1016800.v1.1
prunus_persica	Prupe.1G334900_v2.0.a1 Prupe.1G334900_v2.0.a1
pyrus_communis	pycom16g01540
rosa_chinensis	RchiOBHm_Chr4g0442641
rosa_laevigata	RLG00000005995
rosa_multiflora	Rmu_ssc0000204.1_g000032
rosa_roxburghii	Rroxscaffold_5G00383450
rosa_rugosa	Rorug04G0341700 Rorug04G0341800.1
rosa_samantha	Rh4AG394300 Rh4BG407300 Rh4CG422700 Rh4DG401300
rosa_wichuraiana	Rw4G034070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1512
Acc65I	GGTACC	1 cut(s)	1957
AccB1I	GGYRCC	1 cut(s)	1957
AccB7I	CCANNNNNTGG	2 cut(s)	1567, 2093
AccI	GTMKAC	1 cut(s)	2230
AccII	CGCG	2 cut(s)	494, 2372
AccIII	TCCGGA	1 cut(s)	427
AciI	CCGC	6 cut(s)	419, 494, 580, 1109, 1475, 2426
AclWI	GGATC	6 cut(s)	113, 509, 1643, 2078, 2104, 2130
AcoI	YGGCCR	1 cut(s)	395
AcsI	RAATTY	6 cut(s)	190, 642, 691, 831, 1267, 1774
AcuI	CTGAAG	1 cut(s)	188
AdeI	CACNNNGTG	1 cut(s)	2201
AfaI	GTAC	3 cut(s)	365, 1959, 2377
AfiI	CCNNNNNNNGG	7 cut(s)	434, 1076, 1567, 1698, 1810, 2093, 2110
AflII	CTTAAG	1 cut(s)	260
AflIII	ACRYGT	1 cut(s)	2307
AgeI	ACCGGT	2 cut(s)	450, 2152
AgsI	TTSAA	9 cut(s)	448, 592, 691, 991, 1660, 1744, 1790, 2042, 2132
AhlI	ACTAGT	1 cut(s)	158
AjnI	CCWGG	2 cut(s)	1156, 1424
Alw21I	GWGCWC	4 cut(s)	71, 142, 912, 2142
Alw26I	GTCTC	5 cut(s)	308, 429, 881, 1106, 1375
AlwI	GGATC	6 cut(s)	113, 509, 1643, 2078, 2104, 2130
AlwNI	CAGNNNCTG	1 cut(s)	1977
Ama87I	CYCGRG	2 cut(s)	311, 800
Aor13HI	TCCGGA	1 cut(s)	427
AoxI	GGCC	3 cut(s)	395, 796, 2338
ApeKI	GCWGC	8 cut(s)	199, 385, 415, 1009, 1012, 1386, 1901, 2443
ApoI	RAATTY	6 cut(s)	190, 642, 691, 831, 1267, 1774
ArsI	GACNNNNNNTTYG	2 cut(s)	748, 780
AseI	ATTAAT	1 cut(s)	1137
AsiGI	ACCGGT	2 cut(s)	450, 2152
Asp700I	GAANNNNTTC	2 cut(s)	587, 672
Asp718I	GGTACC	1 cut(s)	1957
AspLEI	GCGC	1 cut(s)	919
AspS9I	GGNCC	3 cut(s)	91, 430, 2339
AsuHPI	GGTGA	1 cut(s)	266
AvaI	CYCGRG	2 cut(s)	311, 800
AvaII	GGWCC	2 cut(s)	91, 430
AxyI	CCTNAGG	1 cut(s)	1749
BaeI	ACNNNNGTAYC	2 cut(s)	1941, 1974
BalI	TGGCCA	1 cut(s)	397
BanI	GGYRCC	1 cut(s)	1957
BanII	GRGCYC	4 cut(s)	142, 685, 912, 1549
BarI	GAAGNNNNNNTAC	2 cut(s)	660, 692
BbsI	GAAGAC	2 cut(s)	78, 2310
Bbv12I	GWGCWC	4 cut(s)	71, 142, 912, 2142
BbvI	GCAGC	8 cut(s)	186, 397, 402, 999, 1021, 1373, 1913, 2430
BccI	CCATC	7 cut(s)	209, 553, 1253, 1465, 1727, 2021, 2350
BceAI	ACGGC	2 cut(s)	1263, 2439
BciT130I	CCWGG	2 cut(s)	1158, 1426
BciVI	GTATCC	1 cut(s)	534
BcoDI	GTCTC	5 cut(s)	308, 429, 881, 1106, 1375
BcuI	ACTAGT	1 cut(s)	158
BfaI	CTAG	3 cut(s)	159, 1364, 1710
BfmI	CTRYAG	3 cut(s)	863, 2444, 2483
BfrI	CTTAAG	1 cut(s)	260
BfuI	GTATCC	1 cut(s)	534
BisI	GCNGC	9 cut(s)	200, 386, 416, 419, 1010, 1013, 1387, 1902, 2444
BlpI	GCTNAGC	2 cut(s)	906, 1905
BlsI	GCNGC	9 cut(s)	201, 387, 417, 420, 1011, 1014, 1388, 1903, 2445
Bme1390I	CCNGG	2 cut(s)	1158, 1426
Bme18I	GGWCC	2 cut(s)	91, 430
BmeT110I	CYCGRG	2 cut(s)	311, 800
BmgT120I	GGNCC	3 cut(s)	91, 430, 2339
BmiI	GGNNCC	3 cut(s)	1479, 1959, 2032
BmrFI	CCNGG	2 cut(s)	1158, 1426
BmrI	ACTGGG	1 cut(s)	1552
BmsI	GCATC	7 cut(s)	18, 1426, 1438, 1763, 2068, 2430, 2465
BmuI	ACTGGG	1 cut(s)	1552
BpiI	GAAGAC	2 cut(s)	78, 2310
BplI	GAGNNNNNCTC	2 cut(s)	1859, 1891
BpmI	CTGGAG	2 cut(s)	1487, 2086
Bpu1102I	GCTNAGC	2 cut(s)	906, 1905
BpuEI	CTTGAG	2 cut(s)	896, 1178
BsaBI	GATNNNNATC	2 cut(s)	552, 2082
BsaJI	CCNNGG	7 cut(s)	72, 433, 897, 1157, 1424, 2109, 2331
BsaWI	WCCGGW	6 cut(s)	102, 346, 427, 450, 838, 2152
BsaXI	ACNNNNNCTCC	4 cut(s)	50, 80, 1103, 1133
Bsc4I	CCNNNNNNNGG	7 cut(s)	434, 1076, 1567, 1698, 1810, 2093, 2110
Bse118I	RCCGGY	3 cut(s)	360, 450, 2152
Bse1I	ACTGG	3 cut(s)	1547, 1997, 2220
Bse21I	CCTNAGG	1 cut(s)	1749
Bse3DI	GCAATG	3 cut(s)	653, 1008, 1458
Bse8I	GATNNNNATC	2 cut(s)	552, 2082
BseAI	TCCGGA	1 cut(s)	427
BseBI	CCWGG	2 cut(s)	1158, 1426
BseDI	CCNNGG	7 cut(s)	72, 433, 897, 1157, 1424, 2109, 2331
BseGI	GGATG	8 cut(s)	190, 232, 1264, 1754, 2083, 2293, 2422, 2445
BseJI	GATNNNNATC	2 cut(s)	552, 2082
BseLI	CCNNNNNNNGG	7 cut(s)	434, 1076, 1567, 1698, 1810, 2093, 2110
BseMI	GCAATG	3 cut(s)	653, 1008, 1458
BseMII	CTCAG	8 cut(s)	155, 897, 1020, 1386, 1763, 1857, 1896, 2457
BseNI	ACTGG	3 cut(s)	1547, 1997, 2220
BseRI	GAGGAG	2 cut(s)	281, 305
BseXI	GCAGC	8 cut(s)	186, 397, 402, 999, 1021, 1373, 1913, 2430
BseYI	CCCAGC	2 cut(s)	1605, 2393
BsgI	GTGCAG	1 cut(s)	1076
Bsh1236I	CGCG	2 cut(s)	494, 2372
Bsh1285I	CGRYCG	2 cut(s)	455, 1928
BshFI	GGCC	3 cut(s)	397, 798, 2340
BshNI	GGYRCC	1 cut(s)	1957
BshTI	ACCGGT	2 cut(s)	450, 2152
BsiEI	CGRYCG	2 cut(s)	455, 1928
BsiHKAI	GWGCWC	4 cut(s)	71, 142, 912, 2142
BsiHKCI	CYCGRG	2 cut(s)	311, 800
BsiSI	CCGG	9 cut(s)	56, 103, 347, 361, 428, 451, 839, 1495, 2153
BslFI	GGGAC	3 cut(s)	314, 1826, 2226
BslI	CCNNNNNNNGG	7 cut(s)	434, 1076, 1567, 1698, 1810, 2093, 2110
BsmAI	GTCTC	5 cut(s)	308, 429, 881, 1106, 1375
BsmFI	GGGAC	3 cut(s)	314, 1826, 2226
BsmI	GAATGC	1 cut(s)	762
BsnI	GGCC	3 cut(s)	397, 798, 2340
BsoBI	CYCGRG	2 cut(s)	311, 800
Bsp1286I	GDGCHC	6 cut(s)	71, 142, 685, 912, 1549, 2142
Bsp13I	TCCGGA	1 cut(s)	427
Bsp1407I	TGTACA	1 cut(s)	2375
Bsp143I	GATC	8 cut(s)	105, 501, 928, 1648, 2083, 2096, 2122, 2294
Bsp1720I	GCTNAGC	2 cut(s)	906, 1905
Bsp19I	CCATGG	2 cut(s)	433, 2331
BspACI	CCGC	6 cut(s)	419, 494, 580, 1109, 1475, 2426
BspANI	GGCC	3 cut(s)	397, 798, 2340
BspCNI	CTCAG	8 cut(s)	154, 898, 1021, 1385, 1762, 1858, 1897, 2458
BspEI	TCCGGA	1 cut(s)	427
BspFNI	CGCG	2 cut(s)	494, 2372
BspHI	TCATGA	2 cut(s)	12, 2001
BspLI	GGNNCC	3 cut(s)	1479, 1959, 2032
BspMAI	CTGCAG	2 cut(s)	867, 2448
BspPI	GGATC	6 cut(s)	113, 509, 1643, 2078, 2104, 2130
BspT107I	GGYRCC	1 cut(s)	1957
BspTI	CTTAAG	1 cut(s)	260
BsrDI	GCAATG	3 cut(s)	653, 1008, 1458
BsrFI	RCCGGY	3 cut(s)	360, 450, 2152
BsrGI	TGTACA	1 cut(s)	2375
BsrI	ACTGG	3 cut(s)	1547, 1997, 2220
BssAI	RCCGGY	3 cut(s)	360, 450, 2152
BssECI	CCNNGG	7 cut(s)	72, 433, 897, 1157, 1424, 2109, 2331
BssMI	GATC	8 cut(s)	105, 501, 928, 1648, 2083, 2096, 2122, 2294
BssT1I	CCWWGG	4 cut(s)	433, 897, 2109, 2331
Bst2UI	CCWGG	2 cut(s)	1158, 1426
Bst4CI	ACNGT	8 cut(s)	19, 596, 820, 1126, 1460, 1926, 1973, 2161
Bst6I	CTCTTC	3 cut(s)	578, 663, 1349
BstAFI	CTTAAG	1 cut(s)	260
BstAUI	TGTACA	1 cut(s)	2375
BstC8I	GCNNGC	4 cut(s)	25, 465, 1549, 2142
BstDSI	CCRYGG	2 cut(s)	433, 2331
BstENI	CCTNNNNNAGG	1 cut(s)	1696
BstF5I	GGATG	8 cut(s)	190, 232, 1264, 1754, 2083, 2293, 2422, 2445
BstFNI	CGCG	2 cut(s)	494, 2372
BstHHI	GCGC	1 cut(s)	919
BstKTI	GATC	8 cut(s)	108, 504, 931, 1651, 2086, 2099, 2125, 2297
BstMAI	GTCTC	5 cut(s)	308, 429, 881, 1106, 1375
BstMBI	GATC	8 cut(s)	105, 501, 928, 1648, 2083, 2096, 2122, 2294
BstMCI	CGRYCG	2 cut(s)	455, 1928
BstMWI	GCNNNNNNNGC	8 cut(s)	205, 394, 902, 916, 1021, 1070, 1504, 2357
BstNI	CCWGG	2 cut(s)	1158, 1426
BstNSI	RCATGY	3 cut(s)	415, 1581, 2311
BstSCI	CCNGG	2 cut(s)	1156, 1424
BstSFI	CTRYAG	3 cut(s)	863, 2444, 2483
BstUI	CGCG	2 cut(s)	494, 2372
BstV1I	GCAGC	8 cut(s)	186, 397, 402, 999, 1021, 1373, 1913, 2430
BstV2I	GAAGAC	2 cut(s)	78, 2310
BstX2I	RGATCY	1 cut(s)	2083
BstXI	CCANNNNNNTGG	1 cut(s)	1535
BstYI	RGATCY	1 cut(s)	2083
Bsu36I	CCTNAGG	1 cut(s)	1749
BsuI	GTATCC	1 cut(s)	534
BsuRI	GGCC	3 cut(s)	397, 798, 2340
BtgI	CCRYGG	2 cut(s)	433, 2331
BtsCI	GGATG	8 cut(s)	190, 232, 1264, 1754, 2083, 2293, 2422, 2445
Cac8I	GCNNGC	4 cut(s)	25, 465, 1549, 2142
CaiI	CAGNNNCTG	1 cut(s)	1977
CciI	TCATGA	2 cut(s)	12, 2001
CfoI	GCGC	1 cut(s)	919
Cfr10I	RCCGGY	3 cut(s)	360, 450, 2152
Cfr13I	GGNCC	3 cut(s)	91, 430, 2339
CseI	GACGC	1 cut(s)	1583
Csp6I	GTAC	3 cut(s)	364, 1958, 2376
CspAI	ACCGGT	2 cut(s)	450, 2152
CviQI	GTAC	3 cut(s)	364, 1958, 2376
DpnI	GATC	8 cut(s)	107, 503, 930, 1650, 2085, 2098, 2124, 2296
DpnII	GATC	8 cut(s)	105, 501, 928, 1648, 2083, 2096, 2122, 2294
DraI	TTTAAA	1 cut(s)	322
DraIII	CACNNNGTG	1 cut(s)	2201
EaeI	YGGCCR	1 cut(s)	395
Eam1104I	CTCTTC	3 cut(s)	578, 663, 1349
EarI	CTCTTC	3 cut(s)	578, 663, 1349
EciI	GGCGGA	3 cut(s)	1124, 1490, 2441
Ecl136II	GAGCTC	2 cut(s)	140, 910
Eco130I	CCWWGG	4 cut(s)	433, 897, 2109, 2331
Eco147I	AGGCCT	1 cut(s)	798
Eco24I	GRGCYC	4 cut(s)	142, 685, 912, 1549
Eco32I	GATATC	1 cut(s)	33
Eco47I	GGWCC	2 cut(s)	91, 430
Eco53kI	GAGCTC	2 cut(s)	140, 910
Eco57I	CTGAAG	1 cut(s)	188
Eco81I	CCTNAGG	1 cut(s)	1749
Eco88I	CYCGRG	2 cut(s)	311, 800
EcoICRI	GAGCTC	2 cut(s)	140, 910
EcoNI	CCTNNNNNAGG	1 cut(s)	1696
EcoRI	GAATTC	2 cut(s)	190, 691
EcoRII	CCWGG	2 cut(s)	1156, 1424
EcoRV	GATATC	1 cut(s)	33
EcoT14I	CCWWGG	4 cut(s)	433, 897, 2109, 2331
EcoT38I	GRGCYC	4 cut(s)	142, 685, 912, 1549
ErhI	CCWWGG	4 cut(s)	433, 897, 2109, 2331
FaqI	GGGAC	3 cut(s)	314, 1826, 2226
FauNDI	CATATG	3 cut(s)	469, 852, 1279
FblI	GTMKAC	1 cut(s)	2230
Fnu4HI	GCNGC	9 cut(s)	200, 386, 416, 419, 1010, 1013, 1387, 1902, 2444
FokI	GGATG	8 cut(s)	197, 239, 1271, 1741, 2090, 2300, 2429, 2452
FriOI	GRGCYC	4 cut(s)	142, 685, 912, 1549
Fsp4HI	GCNGC	9 cut(s)	200, 386, 416, 419, 1010, 1013, 1387, 1902, 2444
FspBI	CTAG	3 cut(s)	159, 1364, 1710
GlaI	GCGC	1 cut(s)	918
GluI	GCNGC	9 cut(s)	200, 386, 416, 419, 1010, 1013, 1387, 1902, 2444
GsaI	CCCAGC	2 cut(s)	1609, 2397
GsuI	CTGGAG	2 cut(s)	1487, 2086
HaeIII	GGCC	3 cut(s)	397, 798, 2340
HapII	CCGG	9 cut(s)	56, 103, 347, 361, 428, 451, 839, 1495, 2153
HgaI	GACGC	1 cut(s)	1583
HhaI	GCGC	1 cut(s)	919
Hin6I	GCGC	1 cut(s)	917
HinP1I	GCGC	1 cut(s)	917
HincII	GTYRAC	2 cut(s)	860, 1122
HindII	GTYRAC	2 cut(s)	860, 1122
HindIII	AAGCTT	2 cut(s)	992, 1889
HinfI	GANTC	8 cut(s)	271, 588, 673, 708, 776, 1337, 1768, 1849
HpaII	CCGG	9 cut(s)	56, 103, 347, 361, 428, 451, 839, 1495, 2153
HphI	GGTGA	1 cut(s)	266
Hpy166II	GTNNAC	6 cut(s)	481, 860, 1122, 1420, 2231, 2376
Hpy8I	GTNNAC	6 cut(s)	481, 860, 1122, 1420, 2231, 2376
Hpy99I	CGWCG	2 cut(s)	85, 337
HpyAV	CCTTC	4 cut(s)	642, 1035, 1404, 2330
HpyCH4III	ACNGT	8 cut(s)	19, 596, 820, 1126, 1460, 1926, 1973, 2161
HpyCH4IV	ACGT	2 cut(s)	335, 2227
HpyF10VI	GCNNNNNNNGC	8 cut(s)	205, 394, 902, 916, 1021, 1070, 1504, 2357
HpySE526I	ACGT	2 cut(s)	335, 2227
HspAI	GCGC	1 cut(s)	917
Kpn2I	TCCGGA	1 cut(s)	427
KpnI	GGTACC	1 cut(s)	1961
Kzo9I	GATC	8 cut(s)	105, 501, 928, 1648, 2083, 2096, 2122, 2294
LmnI	GCTCC	8 cut(s)	74, 349, 1006, 1394, 1406, 1477, 1497, 2356
Lsp1109I	GCAGC	8 cut(s)	186, 397, 402, 999, 1021, 1373, 1913, 2430
LweI	GCATC	7 cut(s)	18, 1426, 1438, 1763, 2068, 2430, 2465
MaeI	CTAG	3 cut(s)	159, 1364, 1710
MaeII	ACGT	2 cut(s)	335, 2227
MaeIII	GTNAC	5 cut(s)	98, 945, 1243, 1538, 2155
MalI	GATC	8 cut(s)	107, 503, 930, 1650, 2085, 2098, 2124, 2296
MboI	GATC	8 cut(s)	105, 501, 928, 1648, 2083, 2096, 2122, 2294
MboII	GAAGA	8 cut(s)	78, 536, 595, 680, 827, 1366, 2054, 2315
MfeI	CAATTG	2 cut(s)	1962, 2346
MflI	RGATCY	1 cut(s)	2083
MhlI	GDGCHC	6 cut(s)	71, 142, 685, 912, 1549, 2142
MlsI	TGGCCA	1 cut(s)	397
MluNI	TGGCCA	1 cut(s)	397
MlyI	GAGTC	2 cut(s)	1777, 1858
MmeI	TCCRAC	2 cut(s)	149, 794
Mox20I	TGGCCA	1 cut(s)	397
MroI	TCCGGA	1 cut(s)	427
MroXI	GAANNNNTTC	2 cut(s)	587, 672
MscI	TGGCCA	1 cut(s)	397
MseI	TTAA	9 cut(s)	261, 321, 516, 828, 975, 1137, 1178, 2163, 2190
MslI	CAYNNNNRTG	4 cut(s)	113, 279, 322, 851
Msp20I	TGGCCA	1 cut(s)	397
MspA1I	CMGCKG	4 cut(s)	208, 421, 1012, 1904
MspCI	CTTAAG	1 cut(s)	260
MspI	CCGG	9 cut(s)	56, 103, 347, 361, 428, 451, 839, 1495, 2153
MspR9I	CCNGG	2 cut(s)	1158, 1426
MunI	CAATTG	2 cut(s)	1962, 2346
Mva1269I	GAATGC	1 cut(s)	762
MvaI	CCWGG	2 cut(s)	1158, 1426
MvnI	CGCG	2 cut(s)	494, 2372
MwoI	GCNNNNNNNGC	8 cut(s)	205, 394, 902, 916, 1021, 1070, 1504, 2357
NcoI	CCATGG	2 cut(s)	433, 2331
NdeI	CATATG	3 cut(s)	469, 852, 1279
NdeII	GATC	8 cut(s)	105, 501, 928, 1648, 2083, 2096, 2122, 2294
NlaIV	GGNNCC	3 cut(s)	1479, 1959, 2032
NmuCI	GTSAC	1 cut(s)	98
NspI	RCATGY	3 cut(s)	415, 1581, 2311
PaeR7I	CTCGAG	2 cut(s)	311, 800
PagI	TCATGA	2 cut(s)	12, 2001
PceI	AGGCCT	1 cut(s)	798
PciI	ACATGT	1 cut(s)	2307
PctI	GAATGC	1 cut(s)	762
PdmI	GAANNNNTTC	2 cut(s)	587, 672
PfeI	GAWTC	6 cut(s)	271, 588, 673, 708, 776, 1337
PflFI	GACNNNGTC	1 cut(s)	1117
PflMI	CCANNNNNTGG	2 cut(s)	1567, 2093
PinAI	ACCGGT	2 cut(s)	450, 2152
PkrI	GCNGC	9 cut(s)	201, 387, 417, 420, 1011, 1014, 1388, 1903, 2445
PleI	GAGTC	2 cut(s)	1776, 1857
PpsI	GAGTC	2 cut(s)	1776, 1857
PscI	ACATGT	1 cut(s)	2307
PshBI	ATTAAT	1 cut(s)	1137
PsiI	TTATAA	1 cut(s)	1512
Psp124BI	GAGCTC	2 cut(s)	142, 912
Psp6I	CCWGG	2 cut(s)	1156, 1424
PspFI	CCCAGC	2 cut(s)	1605, 2393
PspGI	CCWGG	2 cut(s)	1156, 1424
PspN4I	GGNNCC	3 cut(s)	1479, 1959, 2032
PspPI	GGNCC	3 cut(s)	91, 430, 2339
PspXI	VCTCGAGB	1 cut(s)	800
PstI	CTGCAG	2 cut(s)	867, 2448
PstNI	CAGNNNCTG	1 cut(s)	1977
PsuI	RGATCY	1 cut(s)	2083
PsyI	GACNNNGTC	1 cut(s)	1117
PvuII	CAGCTG	3 cut(s)	208, 1012, 1904
RsaI	GTAC	3 cut(s)	365, 1959, 2377
RsaNI	GTAC	3 cut(s)	364, 1958, 2376
RseI	CAYNNNNRTG	4 cut(s)	113, 279, 322, 851
SacI	GAGCTC	2 cut(s)	142, 912
SaqAI	TTAA	9 cut(s)	261, 321, 516, 828, 975, 1137, 1178, 2163, 2190
SatI	GCNGC	9 cut(s)	200, 386, 416, 419, 1010, 1013, 1387, 1902, 2444
Sau3AI	GATC	8 cut(s)	105, 501, 928, 1648, 2083, 2096, 2122, 2294
Sau96I	GGNCC	3 cut(s)	91, 430, 2339
SchI	GAGTC	2 cut(s)	1777, 1858
ScrFI	CCNGG	2 cut(s)	1158, 1426
SduI	GDGCHC	6 cut(s)	71, 142, 685, 912, 1549, 2142
SfaNI	GCATC	7 cut(s)	18, 1426, 1438, 1763, 2068, 2430, 2465
SfcI	CTRYAG	3 cut(s)	863, 2444, 2483
Sfr274I	CTCGAG	2 cut(s)	311, 800
SinI	GGWCC	2 cut(s)	91, 430
SlaI	CTCGAG	2 cut(s)	311, 800
SmiI	ATTTAAAT	1 cut(s)	322
SmiMI	CAYNNNNRTG	4 cut(s)	113, 279, 322, 851
SmlI	CTYRAG	5 cut(s)	260, 311, 800, 911, 1193
SmoI	CTYRAG	5 cut(s)	260, 311, 800, 911, 1193
SpeI	ACTAGT	1 cut(s)	158
SseBI	AGGCCT	1 cut(s)	798
SsiI	CCGC	6 cut(s)	419, 494, 580, 1109, 1475, 2426
SspI	AATATT	1 cut(s)	1432
SspMI	CTAG	3 cut(s)	159, 1364, 1710
SstI	GAGCTC	2 cut(s)	142, 912
StuI	AGGCCT	1 cut(s)	798
StyD4I	CCNGG	2 cut(s)	1156, 1424
StyI	CCWWGG	4 cut(s)	433, 897, 2109, 2331
SwaI	ATTTAAAT	1 cut(s)	322
TaaI	ACNGT	8 cut(s)	19, 596, 820, 1126, 1460, 1926, 1973, 2161
TaiI	ACGT	2 cut(s)	338, 2230
TaqI	TCGA	7 cut(s)	312, 754, 801, 931, 1725, 1852, 1928
TaqII	GACCGA	2 cut(s)	79, 2307
TatI	WGTACW	1 cut(s)	2375
TauI	GCSGC	1 cut(s)	421
TfiI	GAWTC	6 cut(s)	271, 588, 673, 708, 776, 1337
Tru1I	TTAA	9 cut(s)	261, 321, 516, 828, 975, 1137, 1178, 2163, 2190
Tru9I	TTAA	9 cut(s)	261, 321, 516, 828, 975, 1137, 1178, 2163, 2190
TseFI	GTSAC	1 cut(s)	98
TseI	GCWGC	8 cut(s)	199, 385, 415, 1009, 1012, 1386, 1901, 2443
Tsp45I	GTSAC	1 cut(s)	98
TspGWI	ACGGA	1 cut(s)	1955
Tth111I	GACNNNGTC	1 cut(s)	1117
Van91I	CCANNNNNTGG	2 cut(s)	1567, 2093
Vha464I	CTTAAG	1 cut(s)	260
VpaK11BI	GGWCC	2 cut(s)	91, 430
VspI	ATTAAT	1 cut(s)	1137
XagI	CCTNNNNNAGG	1 cut(s)	1696
XapI	RAATTY	6 cut(s)	190, 642, 691, 831, 1267, 1774
XceI	RCATGY	3 cut(s)	415, 1581, 2311
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2116, 2410
XhoI	CTCGAG	2 cut(s)	311, 800
XmiI	GTMKAC	1 cut(s)	2230
XmnI	GAANNNNTTC	2 cut(s)	587, 672
XspI	CTAG	3 cut(s)	159, 1364, 1710

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.