Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0006443

Physical Location & Seq

Genomic Coordinates Forward (+)

1251285 .. 1255787

Gene Structure 4503 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1016800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2547 bp

ATGGTGTCGAAATCGTATTCCAATCTTTTGGAGCTTGCGTCCGGCGAGGGAGACTCGTTTGGCCGAATTGGCCGGCGAATCCCACGAGTTATGAATGTGGCCGGCGTGATGTCCGATGTGGATGACGAAGGGTACACGTCCTCATCGTCCAGCCAACGTGACCGAATCATCATTGTGGCCAACCAGCTCCCCATAAGAGCTCAGAGGAAGGCGGATGACAGTAAGGGTTGGATTTTCAGTTGGGATGAGAATTCACTACTTCTCCAACTGAAAGATGGTTTGGGGGATGATGAGATTGAAGTGATTTATGTTGGTTGTCTTAAGGAGGAGATTCACCCGAACGAACAAGATGAGGTTTCCCAAATCCTCCTCGAGACCTTTAAATGTGTCCCAACCTTTCTCCCACCAGATCTTTTCAGCCGCTACTATCATGGGTTTTGCAAGCAACAGTTGTGGCCGCTGTTTCATTACATGCTGCCCCTTTCGCCGGACCTCGGTGGTAGGTTCAACCGGTCATTATGGCAAGCGTATGTGTCAGTGAACAAGATTTTTGCAGACAGGATCATGGAAGTGATTAACCCAGAAGATGATTTTGTCTGGGTACATGACTATCATCTGATGGTGTTGCCGACCTTTTTGAGGAAGAGATTCAACCGTGCGAAACTTGGGTTTTTCCTCCACAGTCCGTTCCCTTCGTCTGAGATTTACAAGACATTGCCTGTTAGGGAAGAGATTCTGAGAGCCCTTTTAAATTCTGATTTGATCGGATTCCATACATTTGACTATGCAAGGCATTTCCTCTCTTGCTGTAGTCGAATGCTTGGTCTTACGTATGAATCCAAGCGGGGTTATATAGGCCTCGAGTATTATGGTCGAACTGTTAGCATCAAGATTCTTCCTGTTGGCATACACATGGGTCAGCTGCAGTCTGTTTTGAGCCTACCAGAAACGGAAGCTAAGGTTGCTGAGCTTATCAAACAGTTCTCTGACCAGGGAAGGACAATGTTACTTGGAGTTGATGACATGGATATTTTCAAGGGTATAAGTCTGAAGCTTTTGGCAATGGAGCAGCTGCTTGTTCAGCATCCAGAGTGGCAGGGGAAGGTCGTGTTGGTGCAGATAGCCAATCCAGCTAGGGGTCGGGGAAAAGACGTGAAAGAAGTTCAGGCAGAGACATCCTCAACTGTGAAGCGAATTAATGAAACGTTTGGGAGACCCGGATATAAGCCTGTCGTGTTGATTGATGAGCCACTCAAGTTCTATGAGAGAATTGCATATTATGTTGTTGCCGAGTGTTGCTTGGTGACCGCTGTCAGGGATGGAATGAATCTAATTCCATATGAATACATAATCGGTCGGCAAGGAAATTCGAAATTGGACAAAGTTTTGGGGTTGGAGCCGTCCATCCCGAAGAAGAGCATGTTAGTTGTGTCCGAGTTCATTGGCTGCTCTCCTTCTTTGAGCGGAGCAATTCGTGTGAACCCCTGGAATATTGATGCTGTGGCAGATGCCATGGACTGTGCCCTGGAGATGGCGGAGCCAGAAAAACAGCTCAGGCACGAGAAGCATTATAGATATGTCAGCACGCATGATGTTGGGTACTGGGCCCGTAGTTTCCTCCAGGATTTGGAAAGGACATGTAAGGGCCATTTGCGTCAGAGGTCCTGGGGTATTGGGTTCGGGTTGAGTTTCAGAGTTGTGGCCCTTGGTCCAAACTTCAGGAAGCTTTCTATGGAGCATATAGTGTCAGCTTACAGGAGGACTGCAACTAGGGCGATCCTTCTCGACTATGATGGCACATTGATGCCTCAGGCTTCCATCGATAAGAGCCCGACTTCTAAATCCATTGAAATCCTGAATAGCTTGTGCAGAGACAAGAACAACATGGTTTTCATTGTTAGTGCCAAAAGTCGCAAAGTACTAGCTGAATGGTTCTCTCCTTGTGAGAAACTTGGAATTGCTGCTGAGCATGGATACTTTCTACGGTTAAAGCGAGATGCAGAATGGGAAACTTGTGTACCAGTTGCGGACAGTAGTTGGAAGCAGATTGCAGAGCCGGTAATGAAGCTTTACACTGAAACAACTGATGGTTCCACCATTGAAGACAAGGAAACTGCACTTGTGTGGTGTTATGAGGATGCGGATCCTGATTTTGGATCATGCCAAGCTAAGGAACTTCTGGATCATCTTGAAAGTGTGCTTGCTAATGAACCAGTTACTGTTAAAACCGGGCAGAATGTAGTTGAGGTTAAGCCACAGGGCGTAAACAAGGGACTTGTAGCAAAACGTCTACTTTCCACCATGCAAGAAAGGGAAATGTCACCAGATTTTGTTCTCTGCATAGGGGATGACCGATCTGATGAAGACATGTTTGAGGTAATTACAAGTTCCATATCAGGCCCATCCATTGCTCCCAGAGCAGAAGTGTTTGCGTGCACAGTTTGCAAAAAACCCAGCAAAGCCAAGTATTATCTGGATGATGTAGCGGAGATTGTTAGGTTGATGCAGGGATTGGCATGTGTCTCTGAGCAAACTGTACCTCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003825 GO:0004805 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016311 GO:0016740 GO:0016757 GO:0016758 GO:0016787 GO:0016788 GO:0016791 GO:0019203 GO:0033554 GO:0034637 GO:0035251 GO:0042578 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044464 GO:0046351 GO:0046527 GO:0050896 GO:0051716 GO:0070413 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

849

Amino Acids

95.9

Weight (kDa)

5.9

Isoelectric Point (pI)

43.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	55 - 544	3.3e-185	Glycosyltransferase family 20
Trehalose_PPase	PF02358	594 - 828	5.2e-81	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006443)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68020 AT1G68020 AT1G68020
fragaria_vesca	FvH4_4g33380 FvH4_4g33380 FvH4_4g33380 FvH4_4g33380 FvH4_4g33380 FvH4_4g33380 FvH4_4g33380 FvH4_4g33380
malus_domestica	MD16G1016800.v1.1
prunus_persica	Prupe.1G334900_v2.0.a1 Prupe.1G334900_v2.0.a1
pyrus_communis	pycom16g01540
rosa_chinensis	RchiOBHm_Chr4g0442641
rosa_laevigata	RLG00000005995
rosa_multiflora	Rmu_ssc0000204.1_g000032
rosa_roxburghii	Rroxscaffold_5G00383450
rosa_rugosa	Rorug04G0341700 Rorug04G0341800.1
rosa_samantha	Rh4AG394300 Rh4BG407300 Rh4CG422700 Rh4DG401300
rosa_wichuraiana	Rw4G034070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1627
AccBSI	CCGCTC	1 cut(s)	1464
AccI	GTMKAC	1 cut(s)	2290
AclI	AACGTT	1 cut(s)	1205
AclWI	GGATC	6 cut(s)	569, 1771, 2138, 2151, 2164, 2190
AcoI	YGGCCR	5 cut(s)	61, 70, 99, 177, 455
AcsI	RAATTY	3 cut(s)	250, 751, 1366
AcuI	CTGAAG	2 cut(s)	1070, 1702
AfaI	GTAC	6 cut(s)	134, 603, 1601, 1920, 2019, 2538
AfiI	CCNNNNNNNGG	9 cut(s)	487, 494, 639, 1136, 1314, 1531, 1627, 2153, 2170
AflII	CTTAAG	1 cut(s)	320
AflIII	ACRYGT	3 cut(s)	135, 1637, 2367
AgeI	ACCGGT	1 cut(s)	510
AgsI	TTSAA	7 cut(s)	299, 508, 652, 1036, 1850, 2102, 2192
AjiI	CACGTC	2 cut(s)	138, 1153
AjnI	CCWGG	5 cut(s)	990, 1484, 1524, 1620, 1664
AjuI	GAANNNNNNNTTGG	4 cut(s)	263, 295, 1094, 1126
AleI	CACNNNNGTG	1 cut(s)	2122
Alw21I	GWGCWC	2 cut(s)	202, 2438
Alw26I	GTCTC	6 cut(s)	45, 368, 1166, 1207, 1866, 2527
Alw44I	GTGCAC	1 cut(s)	2434
AlwI	GGATC	6 cut(s)	569, 1771, 2138, 2151, 2164, 2190
AlwNI	CAGNNNCTG	1 cut(s)	2219
Ama87I	CYCGRG	2 cut(s)	371, 860
ApaI	GGGCCC	1 cut(s)	1609
ApaLI	GTGCAC	1 cut(s)	2434
ApeKI	GCWGC	6 cut(s)	475, 922, 1069, 1072, 1446, 1961
ApoI	RAATTY	3 cut(s)	250, 751, 1366
ArsI	GACNNNNNNTTYG	2 cut(s)	808, 840
AseI	ATTAAT	1 cut(s)	1197
AsiGI	ACCGGT	1 cut(s)	510
Asp700I	GAANNNNTTC	2 cut(s)	647, 732
AspS9I	GGNCC	8 cut(s)	490, 1605, 1606, 1645, 1662, 1702, 1709, 2399
AsuC2I	CCSGG	2 cut(s)	1218, 2230
AsuHPI	GGTGA	3 cut(s)	326, 1315, 2313
AsuII	TTCGAA	1 cut(s)	1370
AvaI	CYCGRG	2 cut(s)	371, 860
AvaII	GGWCC	3 cut(s)	490, 1662, 1709
AxyI	CCTNAGG	1 cut(s)	1809
BaeGI	GKGCMC	3 cut(s)	1525, 1609, 2438
BaeI	ACNNNNGTAYC	2 cut(s)	2001, 2034
BalI	TGGCCA	1 cut(s)	179
BamHI	GGATCC	1 cut(s)	2143
BanII	GRGCYC	4 cut(s)	202, 745, 1609, 1832
BauI	CACGAG	2 cut(s)	84, 1559
BbsI	GAAGAC	2 cut(s)	2109, 2370
Bbv12I	GWGCWC	2 cut(s)	202, 2438
BbvI	GCAGC	6 cut(s)	462, 909, 1059, 1081, 1433, 1948
BccI	CCATC	9 cut(s)	269, 613, 1313, 1412, 1525, 1787, 1826, 2081, 2410
BceAI	ACGGC	1 cut(s)	1384
BciT130I	CCWGG	5 cut(s)	992, 1486, 1526, 1622, 1666
BciVI	GTATCC	1 cut(s)	1967
BcnI	CCSGG	2 cut(s)	1218, 2230
BcoDI	GTCTC	6 cut(s)	45, 368, 1166, 1207, 1866, 2527
BfaI	CTAG	3 cut(s)	1134, 1770, 1922
BfmI	CTRYAG	2 cut(s)	808, 923
BfrI	CTTAAG	1 cut(s)	320
BfuI	GTATCC	1 cut(s)	1967
BglI	GCCNNNNNGGC	1 cut(s)	69
BglII	AGATCT	1 cut(s)	409
BisI	GCNGC	8 cut(s)	421, 458, 476, 923, 1070, 1073, 1447, 1962
BlpI	GCTNAGC	2 cut(s)	966, 1965
BlsI	GCNGC	8 cut(s)	422, 459, 477, 924, 1071, 1074, 1448, 1963
BmcAI	AGTACT	1 cut(s)	1920
Bme1390I	CCNGG	7 cut(s)	992, 1218, 1486, 1526, 1622, 1666, 2230
Bme18I	GGWCC	3 cut(s)	490, 1662, 1709
BmeT110I	CYCGRG	2 cut(s)	371, 860
BmgBI	CACGTC	2 cut(s)	138, 1153
BmgT120I	GGNCC	8 cut(s)	490, 1605, 1606, 1645, 1662, 1702, 1709, 2399
BmiI	GGNNCC	5 cut(s)	1398, 1539, 1607, 2092, 2145
BmrFI	CCNGG	7 cut(s)	992, 1218, 1486, 1526, 1622, 1666, 2230
BmrI	ACTGGG	1 cut(s)	1612
BmsI	GCATC	8 cut(s)	894, 1093, 1486, 1498, 1794, 1987, 2128, 2493
BmuI	ACTGGG	1 cut(s)	1612
BoxI	GACNNNNGTC	1 cut(s)	1310
BpiI	GAAGAC	2 cut(s)	2109, 2370
BplI	GAGNNNNNCTC	4 cut(s)	38, 70, 1163, 1195
BpmI	CTGGAG	2 cut(s)	1547, 1604
Bpu10I	CCTNAGC	3 cut(s)	957, 1553, 2169
Bpu1102I	GCTNAGC	2 cut(s)	966, 1965
Bpu14I	TTCGAA	1 cut(s)	1370
BpuEI	CTTGAG	1 cut(s)	1238
BpuMI	CCSGG	2 cut(s)	1218, 2230
Bsa29I	ATCGAT	1 cut(s)	1821
BsaAI	YACGTR	1 cut(s)	831
BsaBI	GATNNNNATC	1 cut(s)	2142
BsaI	GGTCTC	2 cut(s)	368, 1207
BsaJI	CCNNGG	7 cut(s)	493, 991, 1484, 1512, 1524, 1665, 1705
BsaWI	WCCGGW	1 cut(s)	510
BsaXI	ACNNNNNCTCC	4 cut(s)	23, 53, 246, 276
Bsc4I	CCNNNNNNNGG	9 cut(s)	487, 494, 639, 1136, 1314, 1531, 1627, 2153, 2170
Bse118I	RCCGGY	4 cut(s)	72, 101, 510, 2056
Bse1I	ACTGG	3 cut(s)	1607, 2021, 2213
Bse21I	CCTNAGG	1 cut(s)	1809
Bse3DI	GCAATG	3 cut(s)	713, 1068, 2406
Bse8I	GATNNNNATC	1 cut(s)	2142
BseBI	CCWGG	5 cut(s)	992, 1486, 1526, 1622, 1666
BseCI	ATCGAT	1 cut(s)	1821
BseDI	CCNNGG	7 cut(s)	493, 991, 1484, 1512, 1524, 1665, 1705
BseJI	GATNNNNATC	1 cut(s)	2142
BseLI	CCNNNNNNNGG	9 cut(s)	487, 494, 639, 1136, 1314, 1531, 1627, 2153, 2170
BseMI	GCAATG	3 cut(s)	713, 1068, 2406
BseMII	CTCAG	8 cut(s)	215, 690, 728, 957, 1567, 1823, 1956, 2517
BseNI	ACTGG	3 cut(s)	1607, 2021, 2213
BseRI	GAGGAG	2 cut(s)	341, 359
BseSI	GKGCMC	3 cut(s)	1525, 1609, 2438
BseXI	GCAGC	6 cut(s)	462, 909, 1059, 1081, 1433, 1948
BseYI	CCCAGC	1 cut(s)	2453
BsgI	GTGCAG	3 cut(s)	1136, 1888, 2100
Bsh1285I	CGRYCG	1 cut(s)	1357
BshTI	ACCGGT	1 cut(s)	510
BshVI	ATCGAT	1 cut(s)	1821
BsiEI	CGRYCG	1 cut(s)	1357
BsiHKAI	GWGCWC	2 cut(s)	202, 2438
BsiHKCI	CYCGRG	2 cut(s)	371, 860
BsiSI	CCGG	8 cut(s)	42, 73, 102, 488, 511, 1218, 2057, 2229
BslFI	GGGAC	2 cut(s)	374, 2286
BslI	CCNNNNNNNGG	9 cut(s)	487, 494, 639, 1136, 1314, 1531, 1627, 2153, 2170
BsmAI	GTCTC	6 cut(s)	45, 368, 1166, 1207, 1866, 2527
BsmFI	GGGAC	2 cut(s)	374, 2286
BsmI	GAATGC	1 cut(s)	822
Bso31I	GGTCTC	2 cut(s)	368, 1207
BsoBI	CYCGRG	2 cut(s)	371, 860
Bsp119I	TTCGAA	1 cut(s)	1370
Bsp120I	GGGCCC	1 cut(s)	1605
Bsp1286I	GDGCHC	6 cut(s)	202, 745, 1525, 1609, 1832, 2438
Bsp143I	GATC	8 cut(s)	409, 561, 762, 1776, 2143, 2156, 2182, 2354
Bsp1720I	GCTNAGC	2 cut(s)	966, 1965
Bsp19I	CCATGG	1 cut(s)	1512
BspCNI	CTCAG	8 cut(s)	214, 691, 729, 958, 1566, 1822, 1957, 2518
BspDI	ATCGAT	1 cut(s)	1821
BspLI	GGNNCC	5 cut(s)	1398, 1539, 1607, 2092, 2145
BspMAI	CTGCAG	1 cut(s)	927
BspPI	GGATC	6 cut(s)	569, 1771, 2138, 2151, 2164, 2190
BspQI	GCTCTTC	1 cut(s)	1409
BspT104I	TTCGAA	1 cut(s)	1370
BspTI	CTTAAG	1 cut(s)	320
BspTNI	GGTCTC	2 cut(s)	368, 1207
BsrBI	CCGCTC	1 cut(s)	1464
BsrDI	GCAATG	3 cut(s)	713, 1068, 2406
BsrFI	RCCGGY	4 cut(s)	72, 101, 510, 2056
BsrI	ACTGG	3 cut(s)	1607, 2021, 2213
BssAI	RCCGGY	4 cut(s)	72, 101, 510, 2056
BssECI	CCNNGG	7 cut(s)	493, 991, 1484, 1512, 1524, 1665, 1705
BssMI	GATC	8 cut(s)	409, 561, 762, 1776, 2143, 2156, 2182, 2354
BssSI	CACGAG	2 cut(s)	84, 1559
BssT1I	CCWWGG	2 cut(s)	1512, 1705
Bst2BI	CACGAG	2 cut(s)	84, 1559
Bst2UI	CCWGG	5 cut(s)	992, 1486, 1526, 1622, 1666
Bst6I	CTCTTC	3 cut(s)	638, 723, 1409
BstAFI	CTTAAG	1 cut(s)	320
BstAPI	GCANNNNNTGC	1 cut(s)	2442
BstBAI	YACGTR	1 cut(s)	831
BstBI	TTCGAA	1 cut(s)	1370
BstC8I	GCNNGC	8 cut(s)	36, 74, 103, 443, 525, 1586, 2202, 2434
BstDSI	CCRYGG	1 cut(s)	1512
BstEII	GGTNACC	1 cut(s)	1303
BstENI	CCTNNNNNAGG	1 cut(s)	637
BstKTI	GATC	8 cut(s)	412, 564, 765, 1779, 2146, 2159, 2185, 2357
BstMAI	GTCTC	6 cut(s)	45, 368, 1166, 1207, 1866, 2527
BstMBI	GATC	8 cut(s)	409, 561, 762, 1776, 2143, 2156, 2182, 2354
BstMCI	CGRYCG	1 cut(s)	1357
BstMWI	GCNNNNNNNGC	9 cut(s)	69, 484, 962, 1081, 1130, 1564, 1772, 2417, 2442
BstNI	CCWGG	5 cut(s)	992, 1486, 1526, 1622, 1666
BstNSI	RCATGY	5 cut(s)	475, 1423, 1641, 2371, 2520
BstPAI	GACNNNNGTC	1 cut(s)	1310
BstPI	GGTNACC	1 cut(s)	1303
BstSCI	CCNGG	7 cut(s)	990, 1216, 1484, 1524, 1620, 1664, 2228
BstSFI	CTRYAG	2 cut(s)	808, 923
BstSLI	GKGCMC	3 cut(s)	1525, 1609, 2438
BstSNI	TACGTA	1 cut(s)	831
BstV1I	GCAGC	6 cut(s)	462, 909, 1059, 1081, 1433, 1948
BstV2I	GAAGAC	2 cut(s)	2109, 2370
BstX2I	RGATCY	2 cut(s)	409, 2143
BstXI	CCANNNNNNTGG	1 cut(s)	28
BstYI	RGATCY	2 cut(s)	409, 2143
Bsu15I	ATCGAT	1 cut(s)	1821
Bsu36I	CCTNAGG	1 cut(s)	1809
BsuI	GTATCC	1 cut(s)	1967
BsuTUI	ATCGAT	1 cut(s)	1821
BtgI	CCRYGG	1 cut(s)	1512
BtrI	CACGTC	2 cut(s)	138, 1153
BtsIMutI	CAGTG	2 cut(s)	543, 2073
Cac8I	GCNNGC	8 cut(s)	36, 74, 103, 443, 525, 1586, 2202, 2434
CaiI	CAGNNNCTG	1 cut(s)	2219
Cfr10I	RCCGGY	4 cut(s)	72, 101, 510, 2056
Cfr13I	GGNCC	8 cut(s)	490, 1605, 1606, 1645, 1662, 1702, 1709, 2399
ClaI	ATCGAT	1 cut(s)	1821
CseI	GACGC	2 cut(s)	27, 1643
Csp6I	GTAC	6 cut(s)	133, 602, 1600, 1919, 2018, 2537
CspAI	ACCGGT	1 cut(s)	510
CspCI	CAANNNNNGTGG	2 cut(s)	434, 469
CviQI	GTAC	6 cut(s)	133, 602, 1600, 1919, 2018, 2537
DpnI	GATC	8 cut(s)	411, 563, 764, 1778, 2145, 2158, 2184, 2356
DpnII	GATC	8 cut(s)	409, 561, 762, 1776, 2143, 2156, 2182, 2354
DraI	TTTAAA	2 cut(s)	382, 750
EaeI	YGGCCR	5 cut(s)	61, 70, 99, 177, 455
Eam1104I	CTCTTC	3 cut(s)	638, 723, 1409
EarI	CTCTTC	3 cut(s)	638, 723, 1409
EciI	GGCGGA	2 cut(s)	227, 1550
Ecl136II	GAGCTC	1 cut(s)	200
Eco105I	TACGTA	1 cut(s)	831
Eco130I	CCWWGG	2 cut(s)	1512, 1705
Eco147I	AGGCCT	1 cut(s)	858
Eco24I	GRGCYC	4 cut(s)	202, 745, 1609, 1832
Eco31I	GGTCTC	2 cut(s)	368, 1207
Eco47I	GGWCC	3 cut(s)	490, 1662, 1709
Eco53kI	GAGCTC	1 cut(s)	200
Eco57I	CTGAAG	2 cut(s)	1070, 1702
Eco81I	CCTNAGG	1 cut(s)	1809
Eco88I	CYCGRG	2 cut(s)	371, 860
Eco91I	GGTNACC	1 cut(s)	1303
EcoICRI	GAGCTC	1 cut(s)	200
EcoNI	CCTNNNNNAGG	1 cut(s)	637
EcoO109I	RGGNCCY	1 cut(s)	1662
EcoO65I	GGTNACC	1 cut(s)	1303
EcoRI	GAATTC	1 cut(s)	250
EcoRII	CCWGG	5 cut(s)	990, 1484, 1524, 1620, 1664
EcoT14I	CCWWGG	2 cut(s)	1512, 1705
EcoT38I	GRGCYC	4 cut(s)	202, 745, 1609, 1832
ErhI	CCWWGG	2 cut(s)	1512, 1705
FaqI	GGGAC	2 cut(s)	374, 2286
FauI	CCCGC	1 cut(s)	837
FauNDI	CATATG	1 cut(s)	1339
FblI	GTMKAC	1 cut(s)	2290
Fnu4HI	GCNGC	8 cut(s)	421, 458, 476, 923, 1070, 1073, 1447, 1962
FriOI	GRGCYC	4 cut(s)	202, 745, 1609, 1832
Fsp4HI	GCNGC	8 cut(s)	421, 458, 476, 923, 1070, 1073, 1447, 1962
FspBI	CTAG	3 cut(s)	1134, 1770, 1922
GluI	GCNGC	8 cut(s)	421, 458, 476, 923, 1070, 1073, 1447, 1962
GsaI	CCCAGC	1 cut(s)	2457
GsuI	CTGGAG	2 cut(s)	1547, 1604
HapII	CCGG	8 cut(s)	42, 73, 102, 488, 511, 1218, 2057, 2229
HgaI	GACGC	2 cut(s)	27, 1643
HindIII	AAGCTT	3 cut(s)	1052, 1724, 2066
HpaII	CCGG	8 cut(s)	42, 73, 102, 488, 511, 1218, 2057, 2229
HphI	GGTGA	3 cut(s)	326, 1315, 2313
Hpy166II	GTNNAC	7 cut(s)	135, 541, 1480, 2018, 2266, 2291, 2436
Hpy8I	GTNNAC	7 cut(s)	135, 541, 1480, 2018, 2266, 2291, 2436
HpyAV	CCTTC	7 cut(s)	122, 202, 702, 990, 1096, 1464, 1790
HpyCH4IV	ACGT	6 cut(s)	137, 157, 830, 1152, 1205, 2287
HpyF10VI	GCNNNNNNNGC	9 cut(s)	69, 484, 962, 1081, 1130, 1564, 1772, 2417, 2442
HpySE526I	ACGT	6 cut(s)	137, 157, 830, 1152, 1205, 2287
KroI	GCCGGC	2 cut(s)	72, 101
KroNI	GCCGGC	2 cut(s)	74, 103
Kzo9I	GATC	8 cut(s)	409, 561, 762, 1776, 2143, 2156, 2182, 2354
LguI	GCTCTTC	1 cut(s)	1409
LmnI	GCTCC	8 cut(s)	31, 192, 1066, 1396, 1466, 1537, 1735, 2416
Lsp1109I	GCAGC	6 cut(s)	462, 909, 1059, 1081, 1433, 1948
LweI	GCATC	8 cut(s)	894, 1093, 1486, 1498, 1794, 1987, 2128, 2493
MaeI	CTAG	3 cut(s)	1134, 1770, 1922
MaeII	ACGT	6 cut(s)	137, 157, 830, 1152, 1205, 2287
MaeIII	GTNAC	5 cut(s)	158, 1005, 1303, 2215, 2319
MalI	GATC	8 cut(s)	411, 563, 764, 1778, 2145, 2158, 2184, 2356
MbiI	CCGCTC	1 cut(s)	1464
MboI	GATC	8 cut(s)	409, 561, 762, 1776, 2143, 2156, 2182, 2354
MboII	GAAGA	8 cut(s)	596, 655, 740, 887, 1423, 1426, 2114, 2375
MflI	RGATCY	2 cut(s)	409, 2143
MhlI	GDGCHC	6 cut(s)	202, 745, 1525, 1609, 1832, 2438
MlsI	TGGCCA	1 cut(s)	179
MluNI	TGGCCA	1 cut(s)	179
MlyI	GAGTC	1 cut(s)	47
MmeI	TCCRAC	4 cut(s)	209, 289, 1374, 2018
Mox20I	TGGCCA	1 cut(s)	179
MroNI	GCCGGC	2 cut(s)	72, 101
MroXI	GAANNNNTTC	2 cut(s)	647, 732
MscI	TGGCCA	1 cut(s)	179
MseI	TTAA	8 cut(s)	321, 381, 576, 749, 1197, 1988, 2223, 2250
MslI	CAYNNNNRTG	5 cut(s)	173, 569, 911, 1802, 2122
Msp20I	TGGCCA	1 cut(s)	179
MspA1I	CMGCKG	4 cut(s)	460, 922, 1072, 1310
MspCI	CTTAAG	1 cut(s)	320
MspI	CCGG	8 cut(s)	42, 73, 102, 488, 511, 1218, 2057, 2229
MspR9I	CCNGG	7 cut(s)	992, 1218, 1486, 1526, 1622, 1666, 2230
Mva1269I	GAATGC	1 cut(s)	822
MvaI	CCWGG	5 cut(s)	992, 1486, 1526, 1622, 1666
MwoI	GCNNNNNNNGC	9 cut(s)	69, 484, 962, 1081, 1130, 1564, 1772, 2417, 2442
NaeI	GCCGGC	2 cut(s)	74, 103
NciI	CCSGG	2 cut(s)	1218, 2230
NcoI	CCATGG	1 cut(s)	1512
NdeI	CATATG	1 cut(s)	1339
NdeII	GATC	8 cut(s)	409, 561, 762, 1776, 2143, 2156, 2182, 2354
NgoMIV	GCCGGC	2 cut(s)	72, 101
NlaIV	GGNNCC	5 cut(s)	1398, 1539, 1607, 2092, 2145
NmeAIII	GCCGAG	1 cut(s)	1315
NmuCI	GTSAC	3 cut(s)	158, 1303, 2319
NspI	RCATGY	5 cut(s)	475, 1423, 1641, 2371, 2520
NspV	TTCGAA	1 cut(s)	1370
OliI	CACNNNNGTG	1 cut(s)	2122
PaeR7I	CTCGAG	2 cut(s)	371, 860
PceI	AGGCCT	1 cut(s)	858
PciI	ACATGT	2 cut(s)	1637, 2367
PciSI	GCTCTTC	1 cut(s)	1409
PcsI	WCGNNNNNNNCGW	2 cut(s)	143, 1990
PctI	GAATGC	1 cut(s)	822
PdiI	GCCGGC	2 cut(s)	74, 103
PdmI	GAANNNNTTC	2 cut(s)	647, 732
PfeI	GAWTC	9 cut(s)	78, 165, 331, 648, 733, 768, 836, 892, 1327
PflMI	CCANNNNNTGG	1 cut(s)	1627
PfoI	TCCNGGA	1 cut(s)	1620
PinAI	ACCGGT	1 cut(s)	510
PkrI	GCNGC	8 cut(s)	422, 459, 477, 924, 1071, 1074, 1448, 1963
PleI	GAGTC	1 cut(s)	47
PpsI	GAGTC	1 cut(s)	47
Ppu21I	YACGTR	1 cut(s)	831
PpuMI	RGGWCCY	1 cut(s)	1662
PscI	ACATGT	2 cut(s)	1637, 2367
PshAI	GACNNNNGTC	1 cut(s)	1310
PshBI	ATTAAT	1 cut(s)	1197
Psp124BI	GAGCTC	1 cut(s)	202
Psp1406I	AACGTT	1 cut(s)	1205
Psp5II	RGGWCCY	1 cut(s)	1662
Psp6I	CCWGG	5 cut(s)	990, 1484, 1524, 1620, 1664
PspEI	GGTNACC	1 cut(s)	1303
PspFI	CCCAGC	1 cut(s)	2453
PspGI	CCWGG	5 cut(s)	990, 1484, 1524, 1620, 1664
PspN4I	GGNNCC	5 cut(s)	1398, 1539, 1607, 2092, 2145
PspOMI	GGGCCC	1 cut(s)	1605
PspPI	GGNCC	8 cut(s)	490, 1605, 1606, 1645, 1662, 1702, 1709, 2399
PspPPI	RGGWCCY	1 cut(s)	1662
PspXI	VCTCGAGB	1 cut(s)	860
PstI	CTGCAG	1 cut(s)	927
PstNI	CAGNNNCTG	1 cut(s)	2219
PsuI	RGATCY	2 cut(s)	409, 2143
PvuII	CAGCTG	2 cut(s)	922, 1072
RsaI	GTAC	6 cut(s)	134, 603, 1601, 1920, 2019, 2538
RsaNI	GTAC	6 cut(s)	133, 602, 1600, 1919, 2018, 2537
RseI	CAYNNNNRTG	5 cut(s)	173, 569, 911, 1802, 2122
SacI	GAGCTC	1 cut(s)	202
SapI	GCTCTTC	1 cut(s)	1409
SaqAI	TTAA	8 cut(s)	321, 381, 576, 749, 1197, 1988, 2223, 2250
SatI	GCNGC	8 cut(s)	421, 458, 476, 923, 1070, 1073, 1447, 1962
Sau3AI	GATC	8 cut(s)	409, 561, 762, 1776, 2143, 2156, 2182, 2354
Sau96I	GGNCC	8 cut(s)	490, 1605, 1606, 1645, 1662, 1702, 1709, 2399
ScaI	AGTACT	1 cut(s)	1920
SchI	GAGTC	1 cut(s)	47
ScrFI	CCNGG	7 cut(s)	992, 1218, 1486, 1526, 1622, 1666, 2230
SduI	GDGCHC	6 cut(s)	202, 745, 1525, 1609, 1832, 2438
SfaNI	GCATC	8 cut(s)	894, 1093, 1486, 1498, 1794, 1987, 2128, 2493
SfcI	CTRYAG	2 cut(s)	808, 923
SfiI	GGCCNNNNNGGCC	1 cut(s)	69
Sfr274I	CTCGAG	2 cut(s)	371, 860
SfuI	TTCGAA	1 cut(s)	1370
SinI	GGWCC	3 cut(s)	490, 1662, 1709
SlaI	CTCGAG	2 cut(s)	371, 860
SmiMI	CAYNNNNRTG	5 cut(s)	173, 569, 911, 1802, 2122
SmlI	CTYRAG	4 cut(s)	320, 371, 860, 1253
SmoI	CTYRAG	4 cut(s)	320, 371, 860, 1253
SnaBI	TACGTA	1 cut(s)	831
SseBI	AGGCCT	1 cut(s)	858
SspI	AATATT	1 cut(s)	1492
SspMI	CTAG	3 cut(s)	1134, 1770, 1922
SstI	GAGCTC	1 cut(s)	202
StuI	AGGCCT	1 cut(s)	858
StyD4I	CCNGG	7 cut(s)	990, 1216, 1484, 1524, 1620, 1664, 2228
StyI	CCWWGG	2 cut(s)	1512, 1705
TaiI	ACGT	6 cut(s)	140, 160, 833, 1155, 1208, 2290
TaqI	TCGA	8 cut(s)	8, 372, 814, 861, 874, 1370, 1785, 1821
TaqII	GACCGA	3 cut(s)	177, 1343, 2367
TatI	WGTACW	1 cut(s)	1918
TauI	GCSGC	2 cut(s)	423, 460
TfiI	GAWTC	9 cut(s)	78, 165, 331, 648, 733, 768, 836, 892, 1327
Tru1I	TTAA	8 cut(s)	321, 381, 576, 749, 1197, 1988, 2223, 2250
Tru9I	TTAA	8 cut(s)	321, 381, 576, 749, 1197, 1988, 2223, 2250
TscAI	CASTG	2 cut(s)	543, 2080
TseFI	GTSAC	3 cut(s)	158, 1303, 2319
TseI	GCWGC	6 cut(s)	475, 922, 1069, 1072, 1446, 1961
Tsp45I	GTSAC	3 cut(s)	158, 1303, 2319
TspGWI	ACGGA	2 cut(s)	675, 965
TspRI	CASTG	2 cut(s)	543, 2080
Van91I	CCANNNNNTGG	1 cut(s)	1627
Vha464I	CTTAAG	1 cut(s)	320
VneI	GTGCAC	1 cut(s)	2434
VpaK11BI	GGWCC	3 cut(s)	490, 1662, 1709
VspI	ATTAAT	1 cut(s)	1197
XagI	CCTNNNNNAGG	1 cut(s)	637
XapI	RAATTY	3 cut(s)	250, 751, 1366
XceI	RCATGY	5 cut(s)	475, 1423, 1641, 2371, 2520
XcmI	CCANNNNNNNNNTGG	2 cut(s)	272, 2470
XhoI	CTCGAG	2 cut(s)	371, 860
XmiI	GTMKAC	1 cut(s)	2290
XmnI	GAANNNNTTC	2 cut(s)	647, 732
XspI	CTAG	3 cut(s)	1134, 1770, 1922
ZrmI	AGTACT	1 cut(s)	1920

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.