Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0006443

Physical Location & Seq

Genomic Coordinates Forward (+)

63044627 .. 63049210

Gene Structure 4584 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00383450.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2565 bp

ATGGTGTCGAAATCGTATTCCAATCTGTTGGAGCTTGCCTCAGGCGAGTCCCCTACCTTCGGCCGAATTGGCCGGAGAATGCCACGCATCATGACTGTTGCTGGCTTGATATCTGATGTTGATGATGACAAGTCGGAGAGTGTGTGCTCCGAGGTGTCCTCATCGTCGGTCCATCGTGACCGAATCATTATAGTGGCAAATCAGCTACCCATCAGGGCTCAGAGAAAAGAAGATTCTAGTAAGGGTTGGATATTCAGTTGGGATGAGAATTCTCTGCTACTCCAACTGAAAGATGGTTTAGGGGATGATGAGATTGAGGTGATTTATGTGGGTTGTCTTAAGGAAGAGATTCACCCCAATGAGCAAGATGAGGTTTCCCAAATCCTCCTTGAGACCTTTAAGTGTGTCCCAACCTTTCTCCCACCAGAGCTTTTCAGCCGGTATTATCATGGATTTTGCAAGCAACAGTTGTGGCCACTCTTTCATTACATGCTACCCCTGTCGCCTGACCTTGGTGGAAGGTTTAACCGGTCGCTGTGGCAAGCATATGTATCAGTGAACAAGATTTTTGCGGATAGGATCATGGAAGTCATTAACCCAGAAGATGATTTTGTGTGGATACATGATTATCATCTGATGGTGTTACCAACTTTCTTGCGCAAGAGATTCAATCGCGTGAAACTTGGGTTTTTCCTCCATAGCCCTTTCCCTTCATCAGAAATTTACAAGACATTGCCTATTAGAGAAGAGATTCTACGAGCCCTTTTGAATTCTGATTTAATTGGATTCCATACATTTGACTATGCAAGGCATTTCCTCTCCTGCTGTAGTCGAATGCTTGGTCTTACCTATGAATCCAAGCGAGGTTATATAGGCCTCGAGTATTATGGTCGGACTGTTAGCATCAAAATTCTTCCGGTTGGCATACATATGGGTCAACTGCAGTCAGTTTTGAGTCTCCCAGAGACAGAAGCCAAGGTTGCTGAGCTCAAGACACAGTTCTGTGATCGGGGTAGGACAATGTTACTTGGAGTGGATGACATGGATATATTTAAGGGCATAAGTTTGAAGCTTTTGGCAATGGAGCAGCTGCTTATTCAGCACCCTGAGTGGCAGGGAAAGGTCGTATTGGTGCAGATAGCCAATCCAGCCAGGGGTCGGGGAAAAGATGTGAAAGAAGTTCAGGCCGAGACATCATCAACTGTGAAGCGAATTAATGAAACATTTGGCAAACCTGGGTATAAGCCTGTTGTCTTGATTGATGAGCCTCTTAAGTTTTATGAGAGAATTGCATACTATGTAGTTGCAGAGTGTTGTTTGGTTACGGCTGTCAGGGATGGAATGAATTTGATACCATATGAATACATTATTAGTCGTCAAGGAAATGAAAAATTGGATAAGGTTTTGGGATTGGATTCCTCTGTTCCCAAGAAGAGTATGTTAGTTGTCTCTGAGTTCATTGGCTGCTCCCCTTCTTTGAGTGGAGCAATTCGTGTGAACCCCTGGAATATTGATGCTGTGGCAGATGCAATGGACTGTTCTCTGGAGATGGCGGAGCCAGAAAAACAGCTTCGGCATGAGAAACATTATAAATATGTCAGCACCCATGATGTTGGTTACTGGGCTCGCAGTTTCCTCCAAGACTTGGAAAGAACATGCAAGGGTCATTTGCGTCAAAGGTGCTGGGGTATTGGGTTTGGGTTGAGTTTTAGAGTTGTGGCTCTTGATCCAAACTTCAAGAAGCTCTCTGTGGAATACATAGTGTCAGCCTACAAAAGGACTACAACTAGGGCAATTCTTCTCGACTATGATGGCACATTGAAGCCTCAGGCATCCATTGATAAGAGTCCAAATTCTAAATCCATCGAAATCCTAAATAGCCTATGCAGGGACAAGAACAACATGGTTTTAATTGTTAGCGCTAAGAGTCGAAAAACACTTGCCGAGTGGTTCTCTCCTTGTGAGAAGCTTGGAATTGCAGCTGAGCATGGCTACTTTCTACGGTCGAAGCAAGATACAGAATGGGAGACATTGGTACCAGTTGCAGACAGTAGCTGGAAGCAGATTGCAGAGCCCGTCATGAAGCTTTACACAGAAACAACTGATGGTTCCACCATTGAAGATAAGGAAACTTCACTTCTCTGGTGTTATGAGGATGCAGATCCAGACTTTGGATCATGCCAAGCCAAGGAACTTATGGATCATCTTGAAAGTGTGCTTGCTAATGAACCTGTTACTGTTAAAAGTGGGCAGACTTTTGTGGAGGTTAAACCACAGGGTGTAAGCAAGGGTCTGGTAGCCAAACGTCTACTTTCCACCATGCAGGAAAGGGGAATGCTACCAGACTTTGTTCTCTGCATAGGGGATGATCGATCTGATGAAGACATGTTTGAGGTAATTACTAGTTCCATGGAAGGCCCATCAATTGCTCCCAGAGCAGAGGTATTCGCGTGTACAGTTTGTCGAAAACCCAGCAAAGCCAAGTATTATCTAGATGACACGGTGGAGATTGTTAGGATGCTGCAGGGTTTGGCATCTGTTTCTGAGCAAAGCATACCTCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003825 GO:0004805 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016311 GO:0016740 GO:0016757 GO:0016758 GO:0016787 GO:0016788 GO:0016791 GO:0019203 GO:0033554 GO:0034637 GO:0035251 GO:0042578 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044464 GO:0046351 GO:0046527 GO:0050896 GO:0051716 GO:0070413 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

854

Amino Acids

96.82

Weight (kDa)

5.84

Isoelectric Point (pI)

44.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	61 - 550	1.6e-186	Glycosyltransferase family 20
Trehalose_PPase	PF02358	600 - 834	1.1e-79	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006443)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68020 AT1G68020 AT1G68020
fragaria_vesca	FvH4_4g33380 FvH4_4g33380 FvH4_4g33380 FvH4_4g33380 FvH4_4g33380 FvH4_4g33380 FvH4_4g33380 FvH4_4g33380
malus_domestica	MD16G1016800.v1.1
prunus_persica	Prupe.1G334900_v2.0.a1 Prupe.1G334900_v2.0.a1
pyrus_communis	pycom16g01540
rosa_chinensis	RchiOBHm_Chr4g0442641
rosa_laevigata	RLG00000005995
rosa_multiflora	Rmu_ssc0000204.1_g000032
rosa_roxburghii	Rroxscaffold_5G00383450
rosa_rugosa	Rorug04G0341700 Rorug04G0341800.1
rosa_samantha	Rh4AG394300 Rh4BG407300 Rh4CG422700 Rh4DG401300
rosa_wichuraiana	Rw4G034070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1590
Acc16I	TGCGCA	1 cut(s)	659
Acc65I	GGTACC	1 cut(s)	2035
AccB1I	GGYRCC	1 cut(s)	2035
AccB7I	CCANNNNNTGG	2 cut(s)	1645, 2171
AccI	GTMKAC	1 cut(s)	2308
AccII	CGCG	2 cut(s)	675, 2450
AciI	CCGC	2 cut(s)	572, 1553
AclWI	GGATC	5 cut(s)	587, 1721, 2156, 2182, 2208
AcoI	YGGCCR	3 cut(s)	61, 70, 473
AcsI	RAATTY	6 cut(s)	268, 720, 769, 909, 1345, 1852
AdeI	CACNNNGTG	1 cut(s)	2279
AfaI	GTAC	2 cut(s)	2037, 2455
AfeI	AGCGCT	1 cut(s)	1921
AfiI	CCNNNNNNNGG	9 cut(s)	59, 512, 1154, 1158, 1645, 1776, 1888, 2171, 2188
AflII	CTTAAG	2 cut(s)	338, 1271
AflIII	ACRYGT	1 cut(s)	2385
AgeI	ACCGGT	1 cut(s)	528
AgsI	TTSAA	7 cut(s)	670, 769, 1069, 1738, 1822, 2120, 2210
AhlI	ACTAGT	1 cut(s)	2402
AjnI	CCWGG	3 cut(s)	1151, 1234, 1502
Alw21I	GWGCWC	2 cut(s)	149, 990
Alw26I	GTCTC	6 cut(s)	386, 959, 962, 1184, 1453, 2021
AlwI	GGATC	5 cut(s)	587, 1721, 2156, 2182, 2208
AlwNI	CAGNNNCTG	1 cut(s)	2055
Ama87I	CYCGRG	1 cut(s)	878
Aor51HI	AGCGCT	1 cut(s)	1921
AoxI	GGCC	6 cut(s)	61, 70, 473, 874, 1185, 2416
ApeKI	GCWGC	5 cut(s)	1087, 1090, 1464, 1979, 2521
ApoI	RAATTY	6 cut(s)	268, 720, 769, 909, 1345, 1852
ArsI	GACNNNNNNTTYG	2 cut(s)	826, 858
AseI	ATTAAT	1 cut(s)	1215
AsiGI	ACCGGT	1 cut(s)	528
Asp700I	GAANNNNTTC	2 cut(s)	348, 750
Asp718I	GGTACC	1 cut(s)	2035
AspLEI	GCGC	2 cut(s)	660, 1922
AspS9I	GGNCC	2 cut(s)	169, 2417
AsuHPI	GGTGA	2 cut(s)	331, 344
AvaI	CYCGRG	1 cut(s)	878
AvaII	GGWCC	1 cut(s)	169
AxyI	CCTNAGG	2 cut(s)	40, 1827
BaeI	ACNNNNGTAYC	2 cut(s)	2019, 2052
BalI	TGGCCA	1 cut(s)	475
BanI	GGYRCC	1 cut(s)	2035
BanII	GRGCYC	5 cut(s)	220, 763, 990, 1627, 2076
BarI	GAAGNNNNNNTAC	2 cut(s)	738, 770
BbsI	GAAGAC	1 cut(s)	2388
Bbv12I	GWGCWC	2 cut(s)	149, 990
BbvI	GCAGC	5 cut(s)	1077, 1099, 1451, 1991, 2508
BceAI	ACGGC	1 cut(s)	1341
BciT130I	CCWGG	3 cut(s)	1153, 1236, 1504
BciVI	GTATCC	1 cut(s)	612
BcoDI	GTCTC	6 cut(s)	386, 959, 962, 1184, 1453, 2021
BcuI	ACTAGT	1 cut(s)	2402
BfaI	CTAG	4 cut(s)	237, 1788, 2403, 2492
BfmI	CTRYAG	4 cut(s)	826, 941, 2522, 2561
BfoI	RGCGCY	1 cut(s)	1923
BfrI	CTTAAG	2 cut(s)	338, 1271
BfuI	GTATCC	1 cut(s)	612
BglI	GCCNNNNNGGC	1 cut(s)	69
BisI	GCNGC	5 cut(s)	1088, 1091, 1465, 1980, 2522
BlpI	GCTNAGC	2 cut(s)	984, 1983
BlsI	GCNGC	5 cut(s)	1089, 1092, 1466, 1981, 2523
Bme1390I	CCNGG	3 cut(s)	1153, 1236, 1504
Bme18I	GGWCC	1 cut(s)	169
BmeT110I	CYCGRG	1 cut(s)	878
BmgT120I	GGNCC	2 cut(s)	169, 2417
BmiI	GGNNCC	3 cut(s)	1557, 2037, 2110
BmrFI	CCNGG	3 cut(s)	1153, 1236, 1504
BmrI	ACTGGG	1 cut(s)	1630
BmsI	GCATC	8 cut(s)	96, 912, 1504, 1516, 1841, 2146, 2508, 2543
BmuI	ACTGGG	1 cut(s)	1630
BpiI	GAAGAC	1 cut(s)	2388
BplI	GAGNNNNNCTC	6 cut(s)	23, 55, 143, 175, 1937, 1969
BpmI	CTGGAG	1 cut(s)	1565
Bpu1102I	GCTNAGC	2 cut(s)	984, 1983
BpuEI	CTTGAG	2 cut(s)	410, 974
Bsa29I	ATCGAT	1 cut(s)	2371
BsaBI	GATNNNNATC	2 cut(s)	630, 2160
BsaI	GGTCTC	1 cut(s)	386
BsaJI	CCNNGG	8 cut(s)	150, 511, 975, 1152, 1235, 1502, 2187, 2409
BsaWI	WCCGGW	2 cut(s)	528, 916
BsaXI	ACNNNNNCTCC	2 cut(s)	128, 158
Bsc4I	CCNNNNNNNGG	9 cut(s)	59, 512, 1154, 1158, 1645, 1776, 1888, 2171, 2188
Bse118I	RCCGGY	2 cut(s)	438, 528
Bse1I	ACTGG	2 cut(s)	1625, 2039
Bse21I	CCTNAGG	2 cut(s)	40, 1827
Bse3DI	GCAATG	3 cut(s)	731, 1086, 1536
Bse8I	GATNNNNATC	2 cut(s)	630, 2160
BseBI	CCWGG	3 cut(s)	1153, 1236, 1504
BseCI	ATCGAT	1 cut(s)	2371
BseDI	CCNNGG	8 cut(s)	150, 511, 975, 1152, 1235, 1502, 2187, 2409
BseGI	GGATG	8 cut(s)	268, 310, 1042, 1342, 1832, 2161, 2371, 2523
BseJI	GATNNNNATC	2 cut(s)	630, 2160
BseLI	CCNNNNNNNGG	9 cut(s)	59, 512, 1154, 1158, 1645, 1776, 1888, 2171, 2188
BseMI	GCAATG	3 cut(s)	731, 1086, 1536
BseMII	CTCAG	8 cut(s)	54, 233, 975, 1098, 1443, 1841, 1974, 2535
BseNI	ACTGG	2 cut(s)	1625, 2039
BseX3I	CGGCCG	1 cut(s)	61
BseXI	GCAGC	5 cut(s)	1077, 1099, 1451, 1991, 2508
BseYI	CCCAGC	2 cut(s)	1683, 2471
BsgI	GTGCAG	1 cut(s)	1154
Bsh1236I	CGCG	2 cut(s)	675, 2450
Bsh1285I	CGRYCG	3 cut(s)	64, 533, 2006
BshFI	GGCC	6 cut(s)	63, 72, 475, 876, 1187, 2418
BshNI	GGYRCC	1 cut(s)	2035
BshTI	ACCGGT	1 cut(s)	528
BshVI	ATCGAT	1 cut(s)	2371
BsiEI	CGRYCG	3 cut(s)	64, 533, 2006
BsiHKAI	GWGCWC	2 cut(s)	149, 990
BsiHKCI	CYCGRG	1 cut(s)	878
BsiSI	CCGG	4 cut(s)	73, 439, 529, 917
BslFI	GGGAC	3 cut(s)	34, 392, 1904
BslI	CCNNNNNNNGG	9 cut(s)	59, 512, 1154, 1158, 1645, 1776, 1888, 2171, 2188
BsmAI	GTCTC	6 cut(s)	386, 959, 962, 1184, 1453, 2021
BsmFI	GGGAC	3 cut(s)	34, 392, 1904
BsmI	GAATGC	3 cut(s)	84, 840, 2340
BsnI	GGCC	6 cut(s)	63, 72, 475, 876, 1187, 2418
Bso31I	GGTCTC	1 cut(s)	386
BsoBI	CYCGRG	1 cut(s)	878
Bsp1286I	GDGCHC	6 cut(s)	149, 220, 763, 990, 1627, 2076
Bsp1407I	TGTACA	1 cut(s)	2453
Bsp143I	GATC	8 cut(s)	579, 1006, 1726, 2161, 2174, 2200, 2368, 2372
Bsp1720I	GCTNAGC	2 cut(s)	984, 1983
Bsp19I	CCATGG	1 cut(s)	2409
BspACI	CCGC	2 cut(s)	572, 1553
BspANI	GGCC	6 cut(s)	63, 72, 475, 876, 1187, 2418
BspCNI	CTCAG	8 cut(s)	53, 232, 976, 1099, 1444, 1840, 1975, 2536
BspDI	ATCGAT	1 cut(s)	2371
BspFNI	CGCG	2 cut(s)	675, 2450
BspHI	TCATGA	2 cut(s)	90, 2079
BspLI	GGNNCC	3 cut(s)	1557, 2037, 2110
BspMAI	CTGCAG	2 cut(s)	945, 2526
BspPI	GGATC	5 cut(s)	587, 1721, 2156, 2182, 2208
BspT107I	GGYRCC	1 cut(s)	2035
BspTI	CTTAAG	2 cut(s)	338, 1271
BspTNI	GGTCTC	1 cut(s)	386
BsrDI	GCAATG	3 cut(s)	731, 1086, 1536
BsrFI	RCCGGY	2 cut(s)	438, 528
BsrGI	TGTACA	1 cut(s)	2453
BsrI	ACTGG	2 cut(s)	1625, 2039
BssAI	RCCGGY	2 cut(s)	438, 528
BssECI	CCNNGG	8 cut(s)	150, 511, 975, 1152, 1235, 1502, 2187, 2409
BssMI	GATC	8 cut(s)	579, 1006, 1726, 2161, 2174, 2200, 2368, 2372
BssT1I	CCWWGG	4 cut(s)	511, 975, 2187, 2409
Bst2UI	CCWGG	3 cut(s)	1153, 1236, 1504
Bst6I	CTCTTC	3 cut(s)	339, 741, 1427
BstAFI	CTTAAG	2 cut(s)	338, 1271
BstAUI	TGTACA	1 cut(s)	2453
BstC8I	GCNNGC	6 cut(s)	36, 103, 461, 543, 1627, 2220
BstDEI	CTNAG	9 cut(s)	40, 219, 984, 1107, 1452, 1827, 1923, 1983, 2544
BstDSI	CCRYGG	1 cut(s)	2409
BstENI	CCTNNNNNAGG	1 cut(s)	1774
BstF5I	GGATG	8 cut(s)	268, 310, 1042, 1342, 1832, 2161, 2371, 2523
BstFNI	CGCG	2 cut(s)	675, 2450
BstH2I	RGCGCY	1 cut(s)	1923
BstHHI	GCGC	2 cut(s)	660, 1922
BstKTI	GATC	8 cut(s)	582, 1009, 1729, 2164, 2177, 2203, 2371, 2375
BstMAI	GTCTC	6 cut(s)	386, 959, 962, 1184, 1453, 2021
BstMBI	GATC	8 cut(s)	579, 1006, 1726, 2161, 2174, 2200, 2368, 2372
BstMCI	CGRYCG	3 cut(s)	64, 533, 2006
BstMWI	GCNNNNNNNGC	5 cut(s)	69, 980, 1099, 1148, 2435
BstNI	CCWGG	3 cut(s)	1153, 1236, 1504
BstNSI	RCATGY	3 cut(s)	493, 1659, 2389
BstSCI	CCNGG	3 cut(s)	1151, 1234, 1502
BstSFI	CTRYAG	4 cut(s)	826, 941, 2522, 2561
BstUI	CGCG	2 cut(s)	675, 2450
BstV1I	GCAGC	5 cut(s)	1077, 1099, 1451, 1991, 2508
BstV2I	GAAGAC	1 cut(s)	2388
BstX2I	RGATCY	1 cut(s)	2161
BstXI	CCANNNNNNTGG	2 cut(s)	28, 1613
BstYI	RGATCY	1 cut(s)	2161
BstZI	CGGCCG	1 cut(s)	61
Bsu15I	ATCGAT	1 cut(s)	2371
Bsu36I	CCTNAGG	2 cut(s)	40, 1827
BsuI	GTATCC	1 cut(s)	612
BsuRI	GGCC	6 cut(s)	63, 72, 475, 876, 1187, 2418
BsuTUI	ATCGAT	1 cut(s)	2371
BtgI	CCRYGG	1 cut(s)	2409
BtsCI	GGATG	8 cut(s)	268, 310, 1042, 1342, 1832, 2161, 2371, 2523
BtsIMutI	CAGTG	1 cut(s)	561
Cac8I	GCNNGC	6 cut(s)	36, 103, 461, 543, 1627, 2220
CaiI	CAGNNNCTG	1 cut(s)	2055
CciI	TCATGA	2 cut(s)	90, 2079
CfoI	GCGC	2 cut(s)	660, 1922
Cfr10I	RCCGGY	2 cut(s)	438, 528
Cfr13I	GGNCC	2 cut(s)	169, 2417
ClaI	ATCGAT	1 cut(s)	2371
CseI	GACGC	1 cut(s)	1661
Csp6I	GTAC	2 cut(s)	2036, 2454
CspAI	ACCGGT	1 cut(s)	528
CspCI	CAANNNNNGTGG	2 cut(s)	452, 487
CviQI	GTAC	2 cut(s)	2036, 2454
DdeI	CTNAG	9 cut(s)	40, 219, 984, 1107, 1452, 1827, 1923, 1983, 2544
DpnI	GATC	8 cut(s)	581, 1008, 1728, 2163, 2176, 2202, 2370, 2374
DpnII	GATC	8 cut(s)	579, 1006, 1726, 2161, 2174, 2200, 2368, 2372
DraIII	CACNNNGTG	1 cut(s)	2279
EaeI	YGGCCR	3 cut(s)	61, 70, 473
EagI	CGGCCG	1 cut(s)	61
Eam1104I	CTCTTC	3 cut(s)	339, 741, 1427
EarI	CTCTTC	3 cut(s)	339, 741, 1427
EciI	GGCGGA	1 cut(s)	1568
Ecl136II	GAGCTC	1 cut(s)	988
EclXI	CGGCCG	1 cut(s)	61
Eco130I	CCWWGG	4 cut(s)	511, 975, 2187, 2409
Eco147I	AGGCCT	1 cut(s)	876
Eco24I	GRGCYC	5 cut(s)	220, 763, 990, 1627, 2076
Eco31I	GGTCTC	1 cut(s)	386
Eco32I	GATATC	1 cut(s)	111
Eco47I	GGWCC	1 cut(s)	169
Eco47III	AGCGCT	1 cut(s)	1921
Eco52I	CGGCCG	1 cut(s)	61
Eco53kI	GAGCTC	1 cut(s)	988
Eco81I	CCTNAGG	2 cut(s)	40, 1827
Eco88I	CYCGRG	1 cut(s)	878
EcoICRI	GAGCTC	1 cut(s)	988
EcoNI	CCTNNNNNAGG	1 cut(s)	1774
EcoRI	GAATTC	2 cut(s)	268, 769
EcoRII	CCWGG	3 cut(s)	1151, 1234, 1502
EcoRV	GATATC	1 cut(s)	111
EcoT14I	CCWWGG	4 cut(s)	511, 975, 2187, 2409
EcoT38I	GRGCYC	5 cut(s)	220, 763, 990, 1627, 2076
ErhI	CCWWGG	4 cut(s)	511, 975, 2187, 2409
FaqI	GGGAC	3 cut(s)	34, 392, 1904
FauNDI	CATATG	3 cut(s)	547, 930, 1357
FblI	GTMKAC	1 cut(s)	2308
Fnu4HI	GCNGC	5 cut(s)	1088, 1091, 1465, 1980, 2522
FokI	GGATG	8 cut(s)	275, 317, 1049, 1349, 1819, 2168, 2378, 2530
FriOI	GRGCYC	5 cut(s)	220, 763, 990, 1627, 2076
Fsp4HI	GCNGC	5 cut(s)	1088, 1091, 1465, 1980, 2522
FspBI	CTAG	4 cut(s)	237, 1788, 2403, 2492
FspI	TGCGCA	1 cut(s)	659
GlaI	GCGC	2 cut(s)	659, 1921
GluI	GCNGC	5 cut(s)	1088, 1091, 1465, 1980, 2522
GsaI	CCCAGC	2 cut(s)	1687, 2475
GsuI	CTGGAG	1 cut(s)	1565
HaeII	RGCGCY	1 cut(s)	1923
HaeIII	GGCC	6 cut(s)	63, 72, 475, 876, 1187, 2418
HapII	CCGG	4 cut(s)	73, 439, 529, 917
HgaI	GACGC	1 cut(s)	1661
HhaI	GCGC	2 cut(s)	660, 1922
Hin6I	GCGC	2 cut(s)	658, 1920
HinP1I	GCGC	2 cut(s)	658, 1920
HincII	GTYRAC	1 cut(s)	938
HindII	GTYRAC	1 cut(s)	938
HindIII	AAGCTT	3 cut(s)	1070, 1967, 2084
HpaII	CCGG	4 cut(s)	73, 439, 529, 917
HphI	GGTGA	2 cut(s)	331, 344
Hpy166II	GTNNAC	5 cut(s)	559, 938, 1498, 2309, 2454
Hpy8I	GTNNAC	5 cut(s)	559, 938, 1498, 2309, 2454
Hpy99I	CGWCG	1 cut(s)	169
HpyAV	CCTTC	5 cut(s)	67, 513, 720, 1482, 2408
HpyCH4IV	ACGT	1 cut(s)	2305
HpyF10VI	GCNNNNNNNGC	5 cut(s)	69, 980, 1099, 1148, 2435
HpyF3I	CTNAG	9 cut(s)	40, 219, 984, 1107, 1452, 1827, 1923, 1983, 2544
HpySE526I	ACGT	1 cut(s)	2305
HspAI	GCGC	2 cut(s)	658, 1920
KpnI	GGTACC	1 cut(s)	2039
Kzo9I	GATC	8 cut(s)	579, 1006, 1726, 2161, 2174, 2200, 2368, 2372
LmnI	GCTCC	7 cut(s)	31, 152, 1084, 1472, 1484, 1555, 2434
Lsp1109I	GCAGC	5 cut(s)	1077, 1099, 1451, 1991, 2508
LweI	GCATC	8 cut(s)	96, 912, 1504, 1516, 1841, 2146, 2508, 2543
MaeI	CTAG	4 cut(s)	237, 1788, 2403, 2492
MaeII	ACGT	1 cut(s)	2305
MaeIII	GTNAC	6 cut(s)	176, 642, 1023, 1321, 1616, 2233
MalI	GATC	8 cut(s)	581, 1008, 1728, 2163, 2176, 2202, 2370, 2374
MboI	GATC	8 cut(s)	579, 1006, 1726, 2161, 2174, 2200, 2368, 2372
MboII	GAAGA	9 cut(s)	242, 356, 614, 758, 905, 1444, 1790, 2132, 2393
MfeI	CAATTG	1 cut(s)	2424
MflI	RGATCY	1 cut(s)	2161
MhlI	GDGCHC	6 cut(s)	149, 220, 763, 990, 1627, 2076
MlsI	TGGCCA	1 cut(s)	475
MluNI	TGGCCA	1 cut(s)	475
MlyI	GAGTC	4 cut(s)	56, 964, 1855, 1936
MmeI	TCCRAC	5 cut(s)	9, 114, 227, 307, 872
Mox20I	TGGCCA	1 cut(s)	475
MroXI	GAANNNNTTC	2 cut(s)	348, 750
MscI	TGGCCA	1 cut(s)	475
MslI	CAYNNNNRTG	3 cut(s)	191, 357, 929
Msp20I	TGGCCA	1 cut(s)	475
MspA1I	CMGCKG	2 cut(s)	1090, 1982
MspCI	CTTAAG	2 cut(s)	338, 1271
MspI	CCGG	4 cut(s)	73, 439, 529, 917
MspR9I	CCNGG	3 cut(s)	1153, 1236, 1504
MunI	CAATTG	1 cut(s)	2424
Mva1269I	GAATGC	3 cut(s)	84, 840, 2340
MvaI	CCWGG	3 cut(s)	1153, 1236, 1504
MvnI	CGCG	2 cut(s)	675, 2450
MwoI	GCNNNNNNNGC	5 cut(s)	69, 980, 1099, 1148, 2435
NcoI	CCATGG	1 cut(s)	2409
NdeI	CATATG	3 cut(s)	547, 930, 1357
NdeII	GATC	8 cut(s)	579, 1006, 1726, 2161, 2174, 2200, 2368, 2372
NlaIV	GGNNCC	3 cut(s)	1557, 2037, 2110
NmeAIII	GCCGAG	2 cut(s)	1213, 1969
NmuCI	GTSAC	1 cut(s)	176
NsbI	TGCGCA	1 cut(s)	659
NspI	RCATGY	3 cut(s)	493, 1659, 2389
PaeR7I	CTCGAG	1 cut(s)	878
PagI	TCATGA	2 cut(s)	90, 2079
PceI	AGGCCT	1 cut(s)	876
PciI	ACATGT	1 cut(s)	2385
PctI	GAATGC	3 cut(s)	84, 840, 2340
PdmI	GAANNNNTTC	2 cut(s)	348, 750
PfeI	GAWTC	8 cut(s)	183, 233, 349, 666, 751, 786, 854, 1415
PflMI	CCANNNNNTGG	2 cut(s)	1645, 2171
PinAI	ACCGGT	1 cut(s)	528
PkrI	GCNGC	5 cut(s)	1089, 1092, 1466, 1981, 2523
PleI	GAGTC	4 cut(s)	55, 963, 1854, 1935
PpsI	GAGTC	4 cut(s)	55, 963, 1854, 1935
PscI	ACATGT	1 cut(s)	2385
PshBI	ATTAAT	1 cut(s)	1215
PsiI	TTATAA	1 cut(s)	1590
Psp124BI	GAGCTC	1 cut(s)	990
Psp6I	CCWGG	3 cut(s)	1151, 1234, 1502
PspFI	CCCAGC	2 cut(s)	1683, 2471
PspGI	CCWGG	3 cut(s)	1151, 1234, 1502
PspN4I	GGNNCC	3 cut(s)	1557, 2037, 2110
PspPI	GGNCC	2 cut(s)	169, 2417
PspXI	VCTCGAGB	1 cut(s)	878
PstI	CTGCAG	2 cut(s)	945, 2526
PstNI	CAGNNNCTG	1 cut(s)	2055
PsuI	RGATCY	1 cut(s)	2161
PvuII	CAGCTG	2 cut(s)	1090, 1982
RsaI	GTAC	2 cut(s)	2037, 2455
RsaNI	GTAC	2 cut(s)	2036, 2454
RseI	CAYNNNNRTG	3 cut(s)	191, 357, 929
SacI	GAGCTC	1 cut(s)	990
SatI	GCNGC	5 cut(s)	1088, 1091, 1465, 1980, 2522
Sau3AI	GATC	8 cut(s)	579, 1006, 1726, 2161, 2174, 2200, 2368, 2372
Sau96I	GGNCC	2 cut(s)	169, 2417
SchI	GAGTC	4 cut(s)	56, 964, 1855, 1936
ScrFI	CCNGG	3 cut(s)	1153, 1236, 1504
SduI	GDGCHC	6 cut(s)	149, 220, 763, 990, 1627, 2076
SfaNI	GCATC	8 cut(s)	96, 912, 1504, 1516, 1841, 2146, 2508, 2543
SfcI	CTRYAG	4 cut(s)	826, 941, 2522, 2561
SfiI	GGCCNNNNNGGCC	1 cut(s)	69
Sfr274I	CTCGAG	1 cut(s)	878
SinI	GGWCC	1 cut(s)	169
SlaI	CTCGAG	1 cut(s)	878
SmiMI	CAYNNNNRTG	3 cut(s)	191, 357, 929
SmlI	CTYRAG	5 cut(s)	338, 389, 878, 989, 1271
SmoI	CTYRAG	5 cut(s)	338, 389, 878, 989, 1271
SpeI	ACTAGT	1 cut(s)	2402
SseBI	AGGCCT	1 cut(s)	876
SsiI	CCGC	2 cut(s)	572, 1553
SspI	AATATT	1 cut(s)	1510
SspMI	CTAG	4 cut(s)	237, 1788, 2403, 2492
SstI	GAGCTC	1 cut(s)	990
StuI	AGGCCT	1 cut(s)	876
StyD4I	CCNGG	3 cut(s)	1151, 1234, 1502
StyI	CCWWGG	4 cut(s)	511, 975, 2187, 2409
TaiI	ACGT	1 cut(s)	2308
TaqI	TCGA	9 cut(s)	8, 832, 879, 1803, 1866, 1930, 2006, 2371, 2464
TaqII	GACCGA	2 cut(s)	157, 195
TatI	WGTACW	1 cut(s)	2453
TfiI	GAWTC	8 cut(s)	183, 233, 349, 666, 751, 786, 854, 1415
TscAI	CASTG	1 cut(s)	561
TseFI	GTSAC	1 cut(s)	176
TseI	GCWGC	5 cut(s)	1087, 1090, 1464, 1979, 2521
Tsp45I	GTSAC	1 cut(s)	176
TspRI	CASTG	1 cut(s)	561
Van91I	CCANNNNNTGG	2 cut(s)	1645, 2171
Vha464I	CTTAAG	2 cut(s)	338, 1271
VpaK11BI	GGWCC	1 cut(s)	169
VspI	ATTAAT	1 cut(s)	1215
XagI	CCTNNNNNAGG	1 cut(s)	1774
XapI	RAATTY	6 cut(s)	268, 720, 769, 909, 1345, 1852
XbaI	TCTAGA	1 cut(s)	2491
XceI	RCATGY	3 cut(s)	493, 1659, 2389
XcmI	CCANNNNNNNNNTGG	2 cut(s)	290, 2194
XhoI	CTCGAG	1 cut(s)	878
XmiI	GTMKAC	1 cut(s)	2308
XmnI	GAANNNNTTC	2 cut(s)	348, 750
XspI	CTAG	4 cut(s)	237, 1788, 2403, 2492

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.