FvH4_4g35421

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0022225
Physical Location & Seq
Forward (+)
32753217 .. 32753781
565 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35421.t1

Sequence Viewer

Length: 450 bp
ATGGGCAAAATGGGCCTAAGCGGGATAGGCGGTTTCCGCTCGTGTCTAGGCGGTTCGGTGGGCCTTGACGGGTTAGACGAAGCAATTAAGGCATCAATGCCGTACGACGGCGCCGTCGGCGGTTTCCGACTCCGGTGGGATGTGTTTCTAAGCTTCAGAGGCGAGGACACCCGCCACACCATCACCAACTCCCTCTACGACGCGCTCGTGCAGCGCGGCGTGCGCGTCTTTCGCGACGACGACGCCCTCAACCGCGGCGACGAGATAGCGCCGAGTCTTCTGGAAGCGATCGACGACTCGGCGGCCGTCGTCGTCGTGCTCTCGTCGAGGTACGCCGACTCCAAGTGGTGCCTTGAGGAGCTCGCGAAGATCTGCTACTGCGGCCGGAGACTGATTCTGCCGGTGTTTTACCAGGTTGAAGGTCCTAGAGGGGGGGTGAATAGGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

150

Amino Acids

16.23

Weight (kDa)

5.41

Isoelectric Point (pI)

49.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 46 - 140 1.1e-27 TIR domain
TIR_2 PF13676 48 - 139 6e-16 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022225)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35421
pyrus_communis pycom16g23320
rosa_multiflora Rmu_sc0003784.1_g000019 Rmu_sc0019418.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 113
AccB1I GGYRCC 2 cut(s) 110, 348
AccBSI CCGCTC 1 cut(s) 39
AccII CGCG 6 cut(s) 203, 216, 225, 234, 255, 365
AcoI YGGCCR 2 cut(s) 303, 382
AcuI CTGAAG 1 cut(s) 139
AcyI GRCGYC 2 cut(s) 111, 243
AfaI GTAC 2 cut(s) 104, 332
AfiI CCNNNNNNNGG 2 cut(s) 107, 431
AgsI TTSAA 1 cut(s) 419
AjnI CCWGG 1 cut(s) 411
AluBI AGCT 2 cut(s) 153, 361
AluI AGCT 2 cut(s) 153, 361
Alw21I GWGCWC 2 cut(s) 321, 363
Alw26I GTCTC 1 cut(s) 382
AoxI GGCC 5 cut(s) 13, 61, 303, 382, 443
ApeKI GCWGC 1 cut(s) 211
AspLEI GCGC 5 cut(s) 113, 205, 216, 225, 271
AspS9I GGNCC 4 cut(s) 13, 61, 422, 444
AsuHPI GGTGA 2 cut(s) 175, 448
AvaII GGWCC 1 cut(s) 422
BanI GGYRCC 2 cut(s) 110, 348
BanII GRGCYC 1 cut(s) 363
BarI GAAGNNNNNNTAC 2 cut(s) 359, 391
BauI CACGAG 2 cut(s) 40, 206
BbsI GAAGAC 1 cut(s) 269
Bbv12I GWGCWC 2 cut(s) 321, 363
BbvI GCAGC 1 cut(s) 223
BccI CCATC 1 cut(s) 188
BceAI ACGGC 4 cut(s) 85, 98, 124, 290
BciT130I CCWGG 1 cut(s) 413
BcoDI GTCTC 1 cut(s) 382
BfaI CTAG 2 cut(s) 47, 426
BfoI RGCGCY 2 cut(s) 114, 272
BglII AGATCT 1 cut(s) 369
BisI GCNGC 5 cut(s) 212, 217, 256, 303, 382
BlsI GCNGC 5 cut(s) 213, 218, 257, 304, 383
Bme1390I CCNGG 1 cut(s) 413
Bme18I GGWCC 1 cut(s) 422
BmgT120I GGNCC 4 cut(s) 13, 61, 422, 444
BmiI GGNNCC 2 cut(s) 112, 350
BmrFI CCNGG 1 cut(s) 413
BmsI GCATC 1 cut(s) 101
BpiI GAAGAC 1 cut(s) 269
Bpu10I CCTNAGC 1 cut(s) 17
BpuEI CTTGAG 1 cut(s) 374
BsaHI GRCGYC 2 cut(s) 111, 243
BsaJI CCNNGG 1 cut(s) 253
BsaWI WCCGGW 1 cut(s) 132
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 431
Bse118I RCCGGY 1 cut(s) 400
BseBI CCWGG 1 cut(s) 413
BseDI CCNNGG 1 cut(s) 253
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 2 cut(s) 107, 431
BseRI GAGGAG 1 cut(s) 371
BseX3I CGGCCG 2 cut(s) 303, 382
BseXI GCAGC 1 cut(s) 223
BsgI GTGCAG 1 cut(s) 230
Bsh1236I CGCG 6 cut(s) 203, 216, 225, 234, 255, 365
Bsh1285I CGRYCG 3 cut(s) 291, 306, 385
BshFI GGCC 5 cut(s) 15, 63, 305, 384, 445
BshNI GGYRCC 2 cut(s) 110, 348
BsiEI CGRYCG 3 cut(s) 291, 306, 385
BsiHKAI GWGCWC 2 cut(s) 321, 363
BsiSI CCGG 3 cut(s) 133, 385, 401
BsiWI CGTACG 1 cut(s) 102
BslI CCNNNNNNNGG 2 cut(s) 107, 431
BsmAI GTCTC 1 cut(s) 382
BsnI GGCC 5 cut(s) 15, 63, 305, 384, 445
Bsp1286I GDGCHC 2 cut(s) 321, 363
Bsp143I GATC 2 cut(s) 288, 369
Bsp68I TCGCGA 2 cut(s) 234, 365
BspANI GGCC 5 cut(s) 15, 63, 305, 384, 445
BspFNI CGCG 6 cut(s) 203, 216, 225, 234, 255, 365
BspLI GGNNCC 2 cut(s) 112, 350
BspT107I GGYRCC 2 cut(s) 110, 348
BsrBI CCGCTC 1 cut(s) 39
BsrFI RCCGGY 1 cut(s) 400
BssAI RCCGGY 1 cut(s) 400
BssECI CCNNGG 1 cut(s) 253
BssMI GATC 2 cut(s) 288, 369
BssNI GRCGYC 2 cut(s) 111, 243
BssSI CACGAG 2 cut(s) 40, 206
Bst2BI CACGAG 2 cut(s) 40, 206
Bst2UI CCWGG 1 cut(s) 413
BstACI GRCGYC 2 cut(s) 111, 243
BstC8I GCNNGC 2 cut(s) 221, 363
BstDEI CTNAG 2 cut(s) 17, 149
BstDSI CCRYGG 1 cut(s) 253
BstF5I GGATG 1 cut(s) 145
BstFNI CGCG 6 cut(s) 203, 216, 225, 234, 255, 365
BstH2I RGCGCY 2 cut(s) 114, 272
BstHHI GCGC 5 cut(s) 113, 205, 216, 225, 271
BstKTI GATC 2 cut(s) 291, 372
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 2 cut(s) 288, 369
BstMCI CGRYCG 3 cut(s) 291, 306, 385
BstNI CCWGG 1 cut(s) 413
BstSCI CCNGG 1 cut(s) 411
BstUI CGCG 6 cut(s) 203, 216, 225, 234, 255, 365
BstV1I GCAGC 1 cut(s) 223
BstV2I GAAGAC 1 cut(s) 269
BstX2I RGATCY 1 cut(s) 369
BstYI RGATCY 1 cut(s) 369
BstZI CGGCCG 2 cut(s) 303, 382
BsuRI GGCC 5 cut(s) 15, 63, 305, 384, 445
BtgI CCRYGG 1 cut(s) 253
BtsCI GGATG 1 cut(s) 145
BtuMI TCGCGA 2 cut(s) 234, 365
Cac8I GCNNGC 2 cut(s) 221, 363
CfoI GCGC 5 cut(s) 113, 205, 216, 225, 271
Cfr10I RCCGGY 1 cut(s) 400
Cfr13I GGNCC 4 cut(s) 13, 61, 422, 444
Cfr42I CCGCGG 1 cut(s) 256
CseI GACGC 3 cut(s) 209, 214, 251
CsiI ACCWGGT 1 cut(s) 411
Csp6I GTAC 2 cut(s) 103, 331
CviJI RGCY 7 cut(s) 15, 63, 153, 305, 361, 384, 445
CviKI_1 RGCY 7 cut(s) 15, 63, 153, 305, 361, 384, 445
CviQI GTAC 2 cut(s) 103, 331
DdeI CTNAG 2 cut(s) 17, 149
DinI GGCGCC 1 cut(s) 112
DpnI GATC 2 cut(s) 290, 371
DpnII GATC 2 cut(s) 288, 369
DrdI GACNNNNNNGTC 1 cut(s) 113
DseDI GACNNNNNNGTC 1 cut(s) 113
EaeI YGGCCR 2 cut(s) 303, 382
EagI CGGCCG 2 cut(s) 303, 382
Ecl136II GAGCTC 1 cut(s) 361
EclXI CGGCCG 2 cut(s) 303, 382
Eco24I GRGCYC 1 cut(s) 363
Eco47I GGWCC 1 cut(s) 422
Eco52I CGGCCG 2 cut(s) 303, 382
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 361
EcoO109I RGGNCCY 2 cut(s) 422, 444
EcoRII CCWGG 1 cut(s) 411
EcoT38I GRGCYC 1 cut(s) 363
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
FauI CCCGC 2 cut(s) 14, 179
Fnu4HI GCNGC 5 cut(s) 212, 217, 256, 303, 382
FokI GGATG 1 cut(s) 152
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 5 cut(s) 212, 217, 256, 303, 382
FspBI CTAG 2 cut(s) 47, 426
GlaI GCGC 5 cut(s) 112, 204, 215, 224, 270
GluI GCNGC 5 cut(s) 212, 217, 256, 303, 382
HaeII RGCGCY 2 cut(s) 114, 272
HaeIII GGCC 5 cut(s) 15, 63, 305, 384, 445
HapII CCGG 3 cut(s) 133, 385, 401
HgaI GACGC 3 cut(s) 209, 214, 251
HhaI GCGC 5 cut(s) 113, 205, 216, 225, 271
Hin1I GRCGYC 2 cut(s) 111, 243
Hin6I GCGC 5 cut(s) 111, 203, 214, 223, 269
HinP1I GCGC 5 cut(s) 111, 203, 214, 223, 269
HindIII AAGCTT 1 cut(s) 151
HinfI GANTC 5 cut(s) 129, 274, 296, 338, 394
HpaII CCGG 3 cut(s) 133, 385, 401
HphI GGTGA 2 cut(s) 175, 448
Hpy188I TCNGA 2 cut(s) 128, 158
Hpy188III TCNNGA 3 cut(s) 233, 281, 364
HpyAV CCTTC 1 cut(s) 413
HpyCH4V TGCA 1 cut(s) 211
HpyF3I CTNAG 2 cut(s) 17, 149
Hsp92I GRCGYC 2 cut(s) 111, 243
HspAI GCGC 5 cut(s) 111, 203, 214, 223, 269
KasI GGCGCC 1 cut(s) 110
KspI CCGCGG 1 cut(s) 256
Kzo9I GATC 2 cut(s) 288, 369
LmnI GCTCC 1 cut(s) 358
LpnPI CCDG 6 cut(s) 146, 266, 398, 398, 414, 425
Lsp1109I GCAGC 1 cut(s) 223
LweI GCATC 1 cut(s) 101
MabI ACCWGGT 1 cut(s) 411
MaeI CTAG 2 cut(s) 47, 426
MalI GATC 2 cut(s) 290, 371
MbiI CCGCTC 1 cut(s) 39
MboI GATC 2 cut(s) 288, 369
MboII GAAGA 2 cut(s) 269, 379
MflI RGATCY 1 cut(s) 369
MhlI GDGCHC 2 cut(s) 321, 363
MluCI AATT 1 cut(s) 84
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 4 cut(s) 123, 283, 290, 332
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 7 cut(s) 152, 157, 203, 257, 321, 349, 422
MseI TTAA 1 cut(s) 87
MspA1I CMGCKG 1 cut(s) 255
MspI CCGG 3 cut(s) 133, 385, 401
MspR9I CCNGG 1 cut(s) 413
MvaI CCWGG 1 cut(s) 413
MvnI CGCG 6 cut(s) 203, 216, 225, 234, 255, 365
NarI GGCGCC 1 cut(s) 111
NdeII GATC 2 cut(s) 288, 369
NlaIV GGNNCC 2 cut(s) 112, 350
NmeAIII GCCGAG 2 cut(s) 278, 297
NruI TCGCGA 2 cut(s) 234, 365
PcsI WCGNNNNNNNCGW 3 cut(s) 75, 111, 204
PfeI GAWTC 1 cut(s) 394
Pfl23II CGTACG 1 cut(s) 102
PkrI GCNGC 5 cut(s) 213, 218, 257, 304, 383
Ple19I CGATCG 1 cut(s) 291
PleI GAGTC 4 cut(s) 123, 282, 290, 332
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 4 cut(s) 123, 282, 290, 332
PpuMI RGGWCCY 1 cut(s) 422
Psp124BI GAGCTC 1 cut(s) 363
Psp5II RGGWCCY 1 cut(s) 422
Psp6I CCWGG 1 cut(s) 411
PspGI CCWGG 1 cut(s) 411
PspLI CGTACG 1 cut(s) 102
PspN4I GGNNCC 2 cut(s) 112, 350
PspPI GGNCC 4 cut(s) 13, 61, 422, 444
PspPPI RGGWCCY 1 cut(s) 422
PsuI RGATCY 1 cut(s) 369
PvuI CGATCG 1 cut(s) 291
RruI TCGCGA 2 cut(s) 234, 365
RsaI GTAC 2 cut(s) 104, 332
RsaNI GTAC 2 cut(s) 103, 331
SacI GAGCTC 1 cut(s) 363
SacII CCGCGG 1 cut(s) 256
SaqAI TTAA 1 cut(s) 87
SatI GCNGC 5 cut(s) 212, 217, 256, 303, 382
Sau3AI GATC 2 cut(s) 288, 369
Sau96I GGNCC 4 cut(s) 13, 61, 422, 444
SchI GAGTC 4 cut(s) 123, 283, 290, 332
ScrFI CCNGG 1 cut(s) 413
SduI GDGCHC 2 cut(s) 321, 363
SetI ASST 5 cut(s) 155, 332, 363, 417, 424
SexAI ACCWGGT 1 cut(s) 411
SfaNI GCATC 1 cut(s) 101
SfoI GGCGCC 1 cut(s) 112
Sfr303I CCGCGG 1 cut(s) 256
SgrBI CCGCGG 1 cut(s) 256
SinI GGWCC 1 cut(s) 422
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 1 cut(s) 84
SspDI GGCGCC 1 cut(s) 110
SspMI CTAG 2 cut(s) 47, 426
SstI GAGCTC 1 cut(s) 363
StyD4I CCNGG 1 cut(s) 411
TaqI TCGA 2 cut(s) 291, 326
TasI AATT 1 cut(s) 84
TauI GCSGC 4 cut(s) 219, 258, 305, 384
TfiI GAWTC 1 cut(s) 394
Tru1I TTAA 1 cut(s) 87
Tru9I TTAA 1 cut(s) 87
TseI GCWGC 1 cut(s) 211
VpaK11BI GGWCC 1 cut(s) 422
XspI CTAG 2 cut(s) 47, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.