pycom16g23320

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0022225
Physical Location & Seq
Reverse (-)
24146150 .. 24148108
1959 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g23320.3

Sequence Viewer

Length: 1134 bp
ATGGCGGGAGAAGACGACGACGGCGACGCCGTTTCGTCAACCCCGGGTGGCTTCCGGCTACGCTGGGACGTGTTCCTGAGTTTCAGAGGAGAAGACACGCGCTCAACAATCACGAAGAGCTTGTACGAGGCCCTCAAAAGCCGCGGCGTCCGAGTCTTCTTAGACGACGACGGTTTGGACCGCGGGGACGATATCGCCCCGAGCCTGCTGGAAGCGATCGACGACTCGGCGGCCGCAATCGTCGTCTTGTCGCGGAGATACGCCGATTCGAGTTGGTGCTTGGAGGAGCTGGCTAAGATATGCGAGAGCAGCAGGAGGTGTCTGATTCTGCCTGTGTTCTACCAAGTGGATCCCTCCGACGTCCGACGACAGAGGGGCCCGTTTTCCGAGCATTTTTCAGCTCACGAACTCCAGTACGGAAACGCCGTCGGCTCGAGGTGGAGGAGCGCCATGGCCAAAGTCGGTGGCAAAGCCGGTTACGTTTGCAACTCCTCCAGCAAGGAAGCAGAGGTGGTTCAGGGGTTGGTCATAAAGGTTTTGAATGAAATAAGGAAAACCCCGGTGGGTCTTGCTTCCTACACAGTGGGACTTGATTCTCGAGTTGAAGATGTGATGAGATTGTTAGATGTTCGATCCAATGGCGTTCAAGTGCTCGGAATTCATGGCATGGGTGGTGTTGGCAAGACGACTCTTGCGAAGGCCCTTTTTAACAGACTTGTTGCTGACTTTTCATATCGCGGCTTCATCTCAAATGTTACAGAAACTTCTGCAGGACACGAGAACAGACTTATCGGTAGTCTTTCTACTCTGAAGATGCCGGTAAATGAACTTAACGCTGGTATTTCTGCAATAAAAGCGACTGTGTACGAGAAGCGAGTTCTTATTGTTTTGGATGATGTTGATAATGTAGAGCAACTTAGTGCTTTAGTTGGCAGCAGAGACTGGTTTTATGAAGGAAGCAGAATCATTGTTACCACAAGAGATAGAAGTGCCTTGACCAATACATATTGTGTCTCTCACAGGTGGGCTACCGTTGGCTCTTCAAGTATTCGGCTCTTACCTATTATATCGGAGGAGAATAGACGAATGGAGAGATTCAATAAAGTTACAAATCGACTGACTGTTAAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

378

Amino Acids

41.54

Weight (kDa)

7.64

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022225)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35421
pyrus_communis pycom16g23320
rosa_multiflora Rmu_sc0003784.1_g000019 Rmu_sc0019418.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 363
AccII CGCG 5 cut(s) 100, 144, 183, 253, 738
AciI CCGC 9 cut(s) 5, 142, 144, 181, 183, 230, 234, 253, 738
AclWI GGATC 3 cut(s) 344, 357, 627
AcoI YGGCCR 2 cut(s) 231, 453
AcsI RAATTY 1 cut(s) 657
AcuI CTGAAG 1 cut(s) 830
AcyI GRCGYC 3 cut(s) 27, 147, 360
AfaI GTAC 3 cut(s) 125, 416, 866
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 5 cut(s) 541, 605, 647, 1044, 1099
AjiI CACGTC 1 cut(s) 70
AluBI AGCT 3 cut(s) 120, 289, 401
AluI AGCT 3 cut(s) 120, 289, 401
Alw21I GWGCWC 1 cut(s) 654
Alw26I GTCTC 2 cut(s) 933, 1018
AlwI GGATC 3 cut(s) 344, 357, 627
AlwNI CAGNNNCTG 1 cut(s) 942
Ama87I CYCGRG 4 cut(s) 43, 199, 433, 597
AoxI GGCC 5 cut(s) 129, 231, 376, 453, 699
ApaI GGGCCC 1 cut(s) 380
ApeKI GCWGC 2 cut(s) 309, 933
ApoI RAATTY 1 cut(s) 657
AspLEI GCGC 2 cut(s) 102, 449
AspS9I GGNCC 5 cut(s) 130, 178, 376, 377, 700
AsuC2I CCSGG 3 cut(s) 44, 45, 560
AvaI CYCGRG 4 cut(s) 43, 199, 433, 597
AvaII GGWCC 1 cut(s) 178
BaeGI GKGCMC 1 cut(s) 380
BalI TGGCCA 1 cut(s) 455
BamHI GGATCC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 380
BarI GAAGNNNNNNTAC 2 cut(s) 107, 139
BauI CACGAG 1 cut(s) 776
BbsI GAAGAC 3 cut(s) 18, 99, 148
Bbv12I GWGCWC 1 cut(s) 654
BbvI GCAGC 2 cut(s) 321, 945
BceAI ACGGC 3 cut(s) 14, 37, 410
BcnI CCSGG 3 cut(s) 44, 45, 560
BcoDI GTCTC 2 cut(s) 933, 1018
BfmI CTRYAG 1 cut(s) 768
BfoI RGCGCY 1 cut(s) 450
BisI GCNGC 7 cut(s) 142, 145, 231, 234, 310, 739, 934
BlsI GCNGC 7 cut(s) 143, 146, 232, 235, 311, 740, 935
Bme1390I CCNGG 3 cut(s) 44, 45, 560
Bme18I GGWCC 1 cut(s) 178
BmeT110I CYCGRG 4 cut(s) 43, 199, 433, 597
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 5 cut(s) 130, 178, 376, 377, 700
BmiI GGNNCC 3 cut(s) 351, 377, 378
BmrFI CCNGG 3 cut(s) 44, 45, 560
BmsI GCATC 1 cut(s) 804
BpiI GAAGAC 3 cut(s) 18, 99, 148
BpmI CTGGAG 2 cut(s) 395, 478
BpuMI CCSGG 3 cut(s) 44, 45, 560
BsaHI GRCGYC 3 cut(s) 27, 147, 360
BsaJI CCNNGG 6 cut(s) 42, 43, 142, 181, 450, 558
Bse118I RCCGGY 2 cut(s) 473, 817
Bse1I ACTGG 2 cut(s) 412, 947
BseDI CCNNGG 6 cut(s) 42, 43, 142, 181, 450, 558
BseGI GGATG 1 cut(s) 898
BseMII CTCAG 1 cut(s) 68
BseNI ACTGG 2 cut(s) 412, 947
BseRI GAGGAG 5 cut(s) 102, 299, 457, 481, 1088
BseSI GKGCMC 1 cut(s) 380
BseX3I CGGCCG 1 cut(s) 231
BseXI GCAGC 2 cut(s) 321, 945
BseYI CCCAGC 1 cut(s) 63
Bsh1236I CGCG 5 cut(s) 100, 144, 183, 253, 738
Bsh1285I CGRYCG 2 cut(s) 219, 234
BshFI GGCC 5 cut(s) 131, 233, 378, 455, 701
BsiEI CGRYCG 2 cut(s) 219, 234
BsiHKAI GWGCWC 1 cut(s) 654
BsiHKCI CYCGRG 4 cut(s) 43, 199, 433, 597
BsiSI CCGG 5 cut(s) 44, 55, 474, 560, 818
BslFI GGGAC 3 cut(s) 80, 200, 600
BsmAI GTCTC 2 cut(s) 933, 1018
BsmFI GGGAC 3 cut(s) 80, 200, 600
BsnI GGCC 5 cut(s) 131, 233, 378, 455, 701
BsoBI CYCGRG 4 cut(s) 43, 199, 433, 597
Bsp120I GGGCCC 1 cut(s) 376
Bsp1286I GDGCHC 2 cut(s) 380, 654
Bsp143I GATC 3 cut(s) 216, 349, 632
Bsp19I CCATGG 1 cut(s) 450
BspACI CCGC 9 cut(s) 5, 142, 144, 181, 183, 230, 234, 253, 738
BspANI GGCC 5 cut(s) 131, 233, 378, 455, 701
BspCNI CTCAG 1 cut(s) 69
BspFNI CGCG 5 cut(s) 100, 144, 183, 253, 738
BspLI GGNNCC 3 cut(s) 351, 377, 378
BspMAI CTGCAG 1 cut(s) 772
BspPI GGATC 3 cut(s) 344, 357, 627
BspQI GCTCTTC 2 cut(s) 110, 1045
BsrFI RCCGGY 2 cut(s) 473, 817
BsrI ACTGG 2 cut(s) 412, 947
BssAI RCCGGY 2 cut(s) 473, 817
BssECI CCNNGG 6 cut(s) 42, 43, 142, 181, 450, 558
BssMI GATC 3 cut(s) 216, 349, 632
BssNI GRCGYC 3 cut(s) 27, 147, 360
BssSI CACGAG 1 cut(s) 776
BssT1I CCWWGG 1 cut(s) 450
Bst2BI CACGAG 1 cut(s) 776
Bst4CI ACNGT 5 cut(s) 173, 583, 862, 1033, 1123
Bst6I CTCTTC 2 cut(s) 110, 1045
BstACI GRCGYC 3 cut(s) 27, 147, 360
BstC8I GCNNGC 2 cut(s) 206, 291
BstDEI CTNAG 4 cut(s) 77, 160, 294, 917
BstDSI CCRYGG 3 cut(s) 142, 181, 450
BstF5I GGATG 1 cut(s) 898
BstFNI CGCG 5 cut(s) 100, 144, 183, 253, 738
BstH2I RGCGCY 1 cut(s) 450
BstHHI GCGC 2 cut(s) 102, 449
BstKTI GATC 3 cut(s) 219, 352, 635
BstMAI GTCTC 2 cut(s) 933, 1018
BstMBI GATC 3 cut(s) 216, 349, 632
BstMCI CGRYCG 2 cut(s) 219, 234
BstMWI GCNNNNNNNGC 2 cut(s) 309, 854
BstSCI CCNGG 3 cut(s) 42, 43, 558
BstSFI CTRYAG 1 cut(s) 768
BstSLI GKGCMC 1 cut(s) 380
BstUI CGCG 5 cut(s) 100, 144, 183, 253, 738
BstV1I GCAGC 2 cut(s) 321, 945
BstV2I GAAGAC 3 cut(s) 18, 99, 148
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BstZI CGGCCG 1 cut(s) 231
BsuRI GGCC 5 cut(s) 131, 233, 378, 455, 701
BtgI CCRYGG 3 cut(s) 142, 181, 450
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 1 cut(s) 898
BtsIMutI CAGTG 1 cut(s) 588
Cac8I GCNNGC 2 cut(s) 206, 291
CaiI CAGNNNCTG 1 cut(s) 942
CciNI GCGGCCGC 1 cut(s) 231
CfoI GCGC 2 cut(s) 102, 449
Cfr10I RCCGGY 2 cut(s) 473, 817
Cfr13I GGNCC 5 cut(s) 130, 178, 376, 377, 700
Cfr42I CCGCGG 2 cut(s) 145, 184
Cfr9I CCCGGG 1 cut(s) 43
CseI GACGC 2 cut(s) 35, 136
Csp6I GTAC 3 cut(s) 124, 415, 865
CspCI CAANNNNNGTGG 2 cut(s) 445, 480
CviAII CATG 3 cut(s) 451, 662, 667
CviQI GTAC 3 cut(s) 124, 415, 865
DdeI CTNAG 4 cut(s) 77, 160, 294, 917
DpnI GATC 3 cut(s) 218, 351, 634
DpnII GATC 3 cut(s) 216, 349, 632
EaeI YGGCCR 2 cut(s) 231, 453
EagI CGGCCG 1 cut(s) 231
Eam1104I CTCTTC 2 cut(s) 110, 1045
EarI CTCTTC 2 cut(s) 110, 1045
EclXI CGGCCG 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 450
Eco24I GRGCYC 1 cut(s) 380
Eco32I GATATC 1 cut(s) 193
Eco47I GGWCC 1 cut(s) 178
Eco52I CGGCCG 1 cut(s) 231
Eco57I CTGAAG 1 cut(s) 830
Eco88I CYCGRG 4 cut(s) 43, 199, 433, 597
EcoO109I RGGNCCY 3 cut(s) 130, 376, 700
EcoRI GAATTC 1 cut(s) 657
EcoRV GATATC 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 450
EcoT38I GRGCYC 1 cut(s) 380
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 3 cut(s) 454, 665, 670
FaiI YATR 9 cut(s) 301, 452, 530, 663, 668, 733, 951, 1006, 1067
FaqI GGGAC 3 cut(s) 80, 200, 600
FatI CATG 3 cut(s) 450, 661, 666
FauI CCCGC 1 cut(s) 176
Fnu4HI GCNGC 7 cut(s) 142, 145, 231, 234, 310, 739, 934
FokI GGATG 1 cut(s) 905
FriOI GRGCYC 1 cut(s) 380
Fsp4HI GCNGC 7 cut(s) 142, 145, 231, 234, 310, 739, 934
GlaI GCGC 2 cut(s) 101, 448
GluI GCNGC 7 cut(s) 142, 145, 231, 234, 310, 739, 934
GsaI CCCAGC 1 cut(s) 67
GsuI CTGGAG 2 cut(s) 395, 478
HaeII RGCGCY 1 cut(s) 450
HaeIII GGCC 5 cut(s) 131, 233, 378, 455, 701
HapII CCGG 5 cut(s) 44, 55, 474, 560, 818
HgaI GACGC 2 cut(s) 35, 136
HhaI GCGC 2 cut(s) 102, 449
Hin1I GRCGYC 3 cut(s) 27, 147, 360
Hin1II CATG 3 cut(s) 454, 665, 670
Hin6I GCGC 2 cut(s) 100, 447
HinP1I GCGC 2 cut(s) 100, 447
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HinfI GANTC 8 cut(s) 153, 224, 266, 325, 593, 688, 963, 1095
HpaII CCGG 5 cut(s) 44, 55, 474, 560, 818
Hpy166II GTNNAC 2 cut(s) 39, 865
Hpy188I TCNGA 9 cut(s) 86, 152, 324, 358, 365, 388, 656, 810, 1072
Hpy188III TCNNGA 4 cut(s) 76, 112, 404, 597
Hpy8I GTNNAC 2 cut(s) 39, 865
HpyAV CCTTC 2 cut(s) 691, 947
HpyCH4III ACNGT 5 cut(s) 173, 583, 862, 1033, 1123
HpyCH4IV ACGT 3 cut(s) 69, 360, 480
HpyCH4V TGCA 3 cut(s) 486, 770, 848
HpyF10VI GCNNNNNNNGC 2 cut(s) 309, 854
HpyF3I CTNAG 4 cut(s) 77, 160, 294, 917
HpySE526I ACGT 3 cut(s) 69, 360, 480
Hsp92I GRCGYC 3 cut(s) 27, 147, 360
Hsp92II CATG 3 cut(s) 454, 665, 670
HspAI GCGC 2 cut(s) 100, 447
KspI CCGCGG 2 cut(s) 145, 184
Kzo9I GATC 3 cut(s) 216, 349, 632
LguI GCTCTTC 2 cut(s) 110, 1045
LmnI GCTCC 2 cut(s) 286, 444
Lsp1109I GCAGC 2 cut(s) 321, 945
LweI GCATC 1 cut(s) 804
MaeII ACGT 3 cut(s) 69, 360, 480
MaeIII GTNAC 4 cut(s) 476, 754, 970, 1105
MalI GATC 3 cut(s) 218, 351, 634
MboI GATC 3 cut(s) 216, 349, 632
MboII GAAGA 7 cut(s) 23, 104, 127, 148, 617, 823, 1032
MflI RGATCY 1 cut(s) 349
MhlI GDGCHC 2 cut(s) 380, 654
MlsI TGGCCA 1 cut(s) 455
MluCI AATT 1 cut(s) 657
MluNI TGGCCA 1 cut(s) 455
MlyI GAGTC 3 cut(s) 162, 218, 682
MmeI TCCRAC 2 cut(s) 381, 388
Mox20I TGGCCA 1 cut(s) 455
MscI TGGCCA 1 cut(s) 455
MseI TTAA 3 cut(s) 708, 831, 1125
Msp20I TGGCCA 1 cut(s) 455
MspA1I CMGCKG 2 cut(s) 144, 183
MspI CCGG 5 cut(s) 44, 55, 474, 560, 818
MspR9I CCNGG 3 cut(s) 44, 45, 560
MvnI CGCG 5 cut(s) 100, 144, 183, 253, 738
MwoI GCNNNNNNNGC 2 cut(s) 309, 854
NciI CCSGG 3 cut(s) 44, 45, 560
NcoI CCATGG 1 cut(s) 450
NdeII GATC 3 cut(s) 216, 349, 632
NlaIII CATG 3 cut(s) 454, 665, 670
NlaIV GGNNCC 3 cut(s) 351, 377, 378
NmeAIII GCCGAG 1 cut(s) 206
NotI GCGGCCGC 1 cut(s) 231
PaeR7I CTCGAG 2 cut(s) 433, 597
PciSI GCTCTTC 2 cut(s) 110, 1045
PcsI WCGNNNNNNNCGW 4 cut(s) 21, 27, 423, 692
PfeI GAWTC 5 cut(s) 266, 325, 593, 963, 1095
PkrI GCNGC 7 cut(s) 143, 146, 232, 235, 311, 740, 935
Ple19I CGATCG 1 cut(s) 219
PleI GAGTC 3 cut(s) 161, 218, 682
PpsI GAGTC 3 cut(s) 161, 218, 682
PspFI CCCAGC 1 cut(s) 63
PspN4I GGNNCC 3 cut(s) 351, 377, 378
PspOMI GGGCCC 1 cut(s) 376
PspPI GGNCC 5 cut(s) 130, 178, 376, 377, 700
PspXI VCTCGAGB 1 cut(s) 433
PstI CTGCAG 1 cut(s) 772
PstNI CAGNNNCTG 1 cut(s) 942
PsuI RGATCY 1 cut(s) 349
PvuI CGATCG 1 cut(s) 219
RsaI GTAC 3 cut(s) 125, 416, 866
RsaNI GTAC 3 cut(s) 124, 415, 865
SacII CCGCGG 2 cut(s) 145, 184
SapI GCTCTTC 2 cut(s) 110, 1045
SaqAI TTAA 3 cut(s) 708, 831, 1125
SatI GCNGC 7 cut(s) 142, 145, 231, 234, 310, 739, 934
Sau3AI GATC 3 cut(s) 216, 349, 632
Sau96I GGNCC 5 cut(s) 130, 178, 376, 377, 700
SchI GAGTC 3 cut(s) 162, 218, 682
ScrFI CCNGG 3 cut(s) 44, 45, 560
SduI GDGCHC 2 cut(s) 380, 654
SfaNI GCATC 1 cut(s) 804
SfcI CTRYAG 1 cut(s) 768
Sfr274I CTCGAG 2 cut(s) 433, 597
Sfr303I CCGCGG 2 cut(s) 145, 184
SgrBI CCGCGG 2 cut(s) 145, 184
SinI GGWCC 1 cut(s) 178
SlaI CTCGAG 2 cut(s) 433, 597
SmaI CCCGGG 1 cut(s) 45
SmlI CTYRAG 2 cut(s) 433, 597
SmoI CTYRAG 2 cut(s) 433, 597
Sse9I AATT 1 cut(s) 657
SsiI CCGC 9 cut(s) 5, 142, 144, 181, 183, 230, 234, 253, 738
StyD4I CCNGG 3 cut(s) 42, 43, 558
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 5 cut(s) 173, 583, 862, 1033, 1123
TaiI ACGT 3 cut(s) 72, 363, 483
TaqI TCGA 6 cut(s) 219, 269, 434, 598, 631, 1114
TasI AATT 1 cut(s) 657
TauI GCSGC 5 cut(s) 144, 147, 233, 236, 741
TfiI GAWTC 5 cut(s) 266, 325, 593, 963, 1095
Tru1I TTAA 3 cut(s) 708, 831, 1125
Tru9I TTAA 3 cut(s) 708, 831, 1125
TscAI CASTG 1 cut(s) 588
TseI GCWGC 2 cut(s) 309, 933
TspDTI ATGAA 6 cut(s) 558, 650, 720, 733, 840, 966
TspGWI ACGGA 1 cut(s) 432
TspMI CCCGGG 1 cut(s) 43
TspRI CASTG 1 cut(s) 588
VpaK11BI GGWCC 1 cut(s) 178
XapI RAATTY 1 cut(s) 657
XhoI CTCGAG 2 cut(s) 433, 597
XmaI CCCGGG 1 cut(s) 43
ZraI GACGTC 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.