Rmu_sc0019418.1_g000001

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0019418.1
OG0022225
Physical Location & Seq
Reverse (-)
2 .. 1062
1061 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0019418.1_g000001.1.cds

Sequence Viewer

Length: 567 bp
atgccgcccgacggcgccgtgggcggtttccggctccggtgggacgtgttcctaagctttagaggagaagacacccgccacaccatcaccaacaacctctacaacgcgctcgaacaacgcggcgtacgagtcttccgcgacgacgacggcctcaaccgcggcgacgacatcgcgccgagtcttctggaagcgatcgaggactcggcggcggccgtcgtcgtgttgtcgccgagatacgccgactcgaggtggtgccttgaggagctcgcgaagatctgcgacggcggccggaaactgatcctgccggtgttttaccaagtggacccctccgacgtccggcgacagggcggaccgtttgaaaaggacttcagatctcatgaagagcgttttgaaagcgacaaggtctctaaatggagaaacgccatggccaaagtaggtggcaaagctggttttgttgtcaacagcagcaacgaagcagagttgattcaatctctagtcaaaacggttttggctgaaataaacaaaatcccggtgggtcttgcagcatacacagtgggtcttgattcc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

189

Amino Acids

20.97

Weight (kDa)

5.32

Isoelectric Point (pI)

53.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022225)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35421
pyrus_communis pycom16g23320
rosa_multiflora Rmu_sc0003784.1_g000019 Rmu_sc0019418.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 336
AccB1I GGYRCC 2 cut(s) 14, 252
AccII CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
AclWI GGATC 1 cut(s) 292
AcoI YGGCCR 3 cut(s) 210, 286, 426
AcuI CTGAAG 1 cut(s) 352
AcyI GRCGYC 2 cut(s) 15, 333
AfaI GTAC 1 cut(s) 126
AfiI CCNNNNNNNGG 4 cut(s) 11, 246, 336, 343
AflIII ACRYGT 1 cut(s) 45
AgsI TTSAA 3 cut(s) 359, 392, 488
AjiI CACGTC 1 cut(s) 46
AluBI AGCT 3 cut(s) 57, 265, 446
AluI AGCT 3 cut(s) 57, 265, 446
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 1 cut(s) 409
AlwI GGATC 1 cut(s) 292
Ama87I CYCGRG 1 cut(s) 244
AoxI GGCC 4 cut(s) 148, 210, 286, 426
ApeKI GCWGC 2 cut(s) 465, 542
AspLEI GCGC 3 cut(s) 17, 109, 175
AspS9I GGNCC 2 cut(s) 322, 350
AsuC2I CCSGG 1 cut(s) 530
AsuHPI GGTGA 1 cut(s) 79
AvaI CYCGRG 1 cut(s) 244
AvaII GGWCC 2 cut(s) 322, 350
BalI TGGCCA 1 cut(s) 428
BanI GGYRCC 2 cut(s) 14, 252
BanII GRGCYC 1 cut(s) 267
BbsI GAAGAC 3 cut(s) 75, 124, 173
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 2 cut(s) 477, 554
BccI CCATC 1 cut(s) 92
BceAI ACGGC 5 cut(s) 2, 28, 163, 197, 298
BcnI CCSGG 1 cut(s) 530
BcoDI GTCTC 1 cut(s) 409
BfaI CTAG 1 cut(s) 494
BfoI RGCGCY 1 cut(s) 18
BglII AGATCT 2 cut(s) 273, 371
BisI GCNGC 8 cut(s) 5, 121, 160, 207, 210, 286, 466, 543
BlsI GCNGC 8 cut(s) 6, 122, 161, 208, 211, 287, 467, 544
Bme1390I CCNGG 1 cut(s) 530
Bme18I GGWCC 2 cut(s) 322, 350
BmeT110I CYCGRG 1 cut(s) 244
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 2 cut(s) 322, 350
BmiI GGNNCC 4 cut(s) 16, 35, 254, 324
BmrFI CCNGG 1 cut(s) 530
BpiI GAAGAC 3 cut(s) 75, 124, 173
Bpu10I CCTNAGC 1 cut(s) 53
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 1 cut(s) 530
BsaHI GRCGYC 2 cut(s) 15, 333
BsaI GGTCTC 1 cut(s) 409
BsaJI CCNNGG 3 cut(s) 18, 157, 423
BsaWI WCCGGW 1 cut(s) 36
Bsc4I CCNNNNNNNGG 4 cut(s) 11, 246, 336, 343
Bse118I RCCGGY 1 cut(s) 304
BseDI CCNNGG 3 cut(s) 18, 157, 423
BseLI CCNNNNNNNGG 4 cut(s) 11, 246, 336, 343
BseRI GAGGAG 2 cut(s) 78, 275
BseX3I CGGCCG 2 cut(s) 210, 286
BseXI GCAGC 2 cut(s) 477, 554
Bsh1236I CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
Bsh1285I CGRYCG 3 cut(s) 195, 213, 289
BshFI GGCC 4 cut(s) 150, 212, 288, 428
BshNI GGYRCC 2 cut(s) 14, 252
BsiEI CGRYCG 3 cut(s) 195, 213, 289
BsiHKAI GWGCWC 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 244
BsiSI CCGG 6 cut(s) 31, 37, 289, 305, 337, 530
BsiWI CGTACG 1 cut(s) 124
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 4 cut(s) 11, 246, 336, 343
BsmAI GTCTC 1 cut(s) 409
BsmFI GGGAC 1 cut(s) 56
BsnI GGCC 4 cut(s) 150, 212, 288, 428
Bso31I GGTCTC 1 cut(s) 409
BsoBI CYCGRG 1 cut(s) 244
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 4 cut(s) 192, 273, 297, 371
Bsp19I CCATGG 1 cut(s) 423
Bsp68I TCGCGA 1 cut(s) 269
BspANI GGCC 4 cut(s) 150, 212, 288, 428
BspFNI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
BspHI TCATGA 1 cut(s) 376
BspLI GGNNCC 4 cut(s) 16, 35, 254, 324
BspPI GGATC 1 cut(s) 292
BspQI GCTCTTC 1 cut(s) 375
BspT107I GGYRCC 2 cut(s) 14, 252
BspTNI GGTCTC 1 cut(s) 409
BsrFI RCCGGY 1 cut(s) 304
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 3 cut(s) 18, 157, 423
BssMI GATC 4 cut(s) 192, 273, 297, 371
BssNI GRCGYC 2 cut(s) 15, 333
BssT1I CCWWGG 1 cut(s) 423
Bst4CI ACNGT 3 cut(s) 354, 505, 553
Bst6I CTCTTC 1 cut(s) 375
BstACI GRCGYC 2 cut(s) 15, 333
BstC8I GCNNGC 1 cut(s) 267
BstDEI CTNAG 1 cut(s) 53
BstDSI CCRYGG 3 cut(s) 18, 157, 423
BstFNI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 3 cut(s) 17, 109, 175
BstKTI GATC 4 cut(s) 195, 276, 300, 374
BstMAI GTCTC 1 cut(s) 409
BstMBI GATC 4 cut(s) 192, 273, 297, 371
BstMCI CGRYCG 3 cut(s) 195, 213, 289
BstMWI GCNNNNNNNGC 3 cut(s) 21, 156, 285
BstSCI CCNGG 1 cut(s) 528
BstUI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
BstV1I GCAGC 2 cut(s) 477, 554
BstV2I GAAGAC 3 cut(s) 75, 124, 173
BstX2I RGATCY 2 cut(s) 273, 371
BstYI RGATCY 2 cut(s) 273, 371
BstZI CGGCCG 2 cut(s) 210, 286
BsuRI GGCC 4 cut(s) 150, 212, 288, 428
BtgI CCRYGG 3 cut(s) 18, 157, 423
BtgZI GCGATG 1 cut(s) 154
BtrI CACGTC 1 cut(s) 46
BtsIMutI CAGTG 1 cut(s) 558
BtuMI TCGCGA 1 cut(s) 269
Cac8I GCNNGC 1 cut(s) 267
CciI TCATGA 1 cut(s) 376
CfoI GCGC 3 cut(s) 17, 109, 175
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 2 cut(s) 322, 350
Cfr42I CCGCGG 1 cut(s) 160
CpoI CGGWCCG 1 cut(s) 350
Csp6I GTAC 1 cut(s) 125
CspCI CAANNNNNGTGG 2 cut(s) 418, 453
CspI CGGWCCG 1 cut(s) 350
CviAII CATG 2 cut(s) 377, 424
CviJI RGCY 9 cut(s) 34, 57, 150, 212, 265, 288, 428, 446, 512
CviKI_1 RGCY 9 cut(s) 34, 57, 150, 212, 265, 288, 428, 446, 512
CviQI GTAC 1 cut(s) 125
DdeI CTNAG 1 cut(s) 53
DinI GGCGCC 1 cut(s) 16
DpnI GATC 4 cut(s) 194, 275, 299, 373
DpnII GATC 4 cut(s) 192, 273, 297, 371
EaeI YGGCCR 3 cut(s) 210, 286, 426
EagI CGGCCG 2 cut(s) 210, 286
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
EciI GGCGGA 1 cut(s) 363
Ecl136II GAGCTC 1 cut(s) 265
EclXI CGGCCG 2 cut(s) 210, 286
Eco130I CCWWGG 1 cut(s) 423
Eco24I GRGCYC 1 cut(s) 267
Eco31I GGTCTC 1 cut(s) 409
Eco47I GGWCC 2 cut(s) 322, 350
Eco52I CGGCCG 2 cut(s) 210, 286
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 352
Eco88I CYCGRG 1 cut(s) 244
EcoICRI GAGCTC 1 cut(s) 265
EcoT14I CCWWGG 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 267
EgeI GGCGCC 1 cut(s) 16
EheI GGCGCC 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 2 cut(s) 380, 427
FaiI YATR 3 cut(s) 378, 425, 547
FaqI GGGAC 1 cut(s) 56
FatI CATG 2 cut(s) 376, 423
FauI CCCGC 1 cut(s) 83
Fnu4HI GCNGC 8 cut(s) 5, 121, 160, 207, 210, 286, 466, 543
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 8 cut(s) 5, 121, 160, 207, 210, 286, 466, 543
FspBI CTAG 1 cut(s) 494
GlaI GCGC 3 cut(s) 16, 108, 174
GluI GCNGC 8 cut(s) 5, 121, 160, 207, 210, 286, 466, 543
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 4 cut(s) 150, 212, 288, 428
HapII CCGG 6 cut(s) 31, 37, 289, 305, 337, 530
HhaI GCGC 3 cut(s) 17, 109, 175
Hin1I GRCGYC 2 cut(s) 15, 333
Hin1II CATG 2 cut(s) 380, 427
Hin6I GCGC 3 cut(s) 15, 107, 173
HinP1I GCGC 3 cut(s) 15, 107, 173
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HindIII AAGCTT 1 cut(s) 55
HinfI GANTC 6 cut(s) 129, 178, 200, 242, 484, 563
HpaII CCGG 6 cut(s) 31, 37, 289, 305, 337, 530
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 2 cut(s) 322, 460
Hpy188I TCNGA 2 cut(s) 331, 371
Hpy188III TCNNGA 4 cut(s) 185, 268, 377, 560
Hpy8I GTNNAC 2 cut(s) 322, 460
Hpy99I CGWCG 9 cut(s) 14, 143, 146, 149, 167, 218, 221, 284, 335
HpyCH4III ACNGT 3 cut(s) 354, 505, 553
HpyCH4IV ACGT 2 cut(s) 45, 333
HpyCH4V TGCA 1 cut(s) 542
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 156, 285
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 2 cut(s) 45, 333
Hsp92I GRCGYC 2 cut(s) 15, 333
Hsp92II CATG 2 cut(s) 380, 427
HspAI GCGC 3 cut(s) 15, 107, 173
KasI GGCGCC 1 cut(s) 14
KspI CCGCGG 1 cut(s) 160
Kzo9I GATC 4 cut(s) 192, 273, 297, 371
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 2 cut(s) 39, 262
Lsp1109I GCAGC 2 cut(s) 477, 554
MaeI CTAG 1 cut(s) 494
MaeII ACGT 2 cut(s) 45, 333
MalI GATC 4 cut(s) 194, 275, 299, 373
MboI GATC 4 cut(s) 192, 273, 297, 371
MboII GAAGA 5 cut(s) 80, 124, 173, 283, 392
MflI RGATCY 2 cut(s) 273, 371
MhlI GDGCHC 1 cut(s) 267
MlsI TGGCCA 1 cut(s) 428
MluNI TGGCCA 1 cut(s) 428
Mly113I GGCGCC 1 cut(s) 15
MlyI GAGTC 4 cut(s) 138, 187, 194, 236
MmeI TCCRAC 1 cut(s) 354
MnlI CCTC 7 cut(s) 56, 107, 161, 190, 240, 253, 337
Mox20I TGGCCA 1 cut(s) 428
MscI TGGCCA 1 cut(s) 428
Msp20I TGGCCA 1 cut(s) 428
MspA1I CMGCKG 1 cut(s) 159
MspI CCGG 6 cut(s) 31, 37, 289, 305, 337, 530
MspR9I CCNGG 1 cut(s) 530
MvnI CGCG 6 cut(s) 107, 120, 138, 159, 173, 269
MwoI GCNNNNNNNGC 3 cut(s) 21, 156, 285
NarI GGCGCC 1 cut(s) 15
NciI CCSGG 1 cut(s) 530
NcoI CCATGG 1 cut(s) 423
NdeII GATC 4 cut(s) 192, 273, 297, 371
NlaIII CATG 2 cut(s) 380, 427
NlaIV GGNNCC 4 cut(s) 16, 35, 254, 324
NmeAIII GCCGAG 3 cut(s) 182, 201, 255
NruI TCGCGA 1 cut(s) 269
PaeR7I CTCGAG 1 cut(s) 244
PagI TCATGA 1 cut(s) 376
PciSI GCTCTTC 1 cut(s) 375
PcsI WCGNNNNNNNCGW 1 cut(s) 124
PfeI GAWTC 2 cut(s) 484, 563
Pfl23II CGTACG 1 cut(s) 124
PflFI GACNNNGTC 1 cut(s) 401
PkrI GCNGC 8 cut(s) 6, 122, 161, 208, 211, 287, 467, 544
Ple19I CGATCG 1 cut(s) 195
PleI GAGTC 4 cut(s) 137, 186, 194, 236
PluTI GGCGCC 1 cut(s) 18
PpsI GAGTC 4 cut(s) 137, 186, 194, 236
Psp124BI GAGCTC 1 cut(s) 267
PspLI CGTACG 1 cut(s) 124
PspN4I GGNNCC 4 cut(s) 16, 35, 254, 324
PspPI GGNCC 2 cut(s) 322, 350
PspXI VCTCGAGB 1 cut(s) 244
PsuI RGATCY 2 cut(s) 273, 371
PsyI GACNNNGTC 1 cut(s) 401
PvuI CGATCG 1 cut(s) 195
RruI TCGCGA 1 cut(s) 269
RsaI GTAC 1 cut(s) 126
RsaNI GTAC 1 cut(s) 125
Rsr2I CGGWCCG 1 cut(s) 350
RsrII CGGWCCG 1 cut(s) 350
SacI GAGCTC 1 cut(s) 267
SacII CCGCGG 1 cut(s) 160
SapI GCTCTTC 1 cut(s) 375
SatI GCNGC 8 cut(s) 5, 121, 160, 207, 210, 286, 466, 543
Sau3AI GATC 4 cut(s) 192, 273, 297, 371
Sau96I GGNCC 2 cut(s) 322, 350
SchI GAGTC 4 cut(s) 138, 187, 194, 236
ScrFI CCNGG 1 cut(s) 530
SduI GDGCHC 1 cut(s) 267
SetI ASST 9 cut(s) 48, 59, 99, 251, 267, 336, 405, 439, 448
SfoI GGCGCC 1 cut(s) 16
Sfr274I CTCGAG 1 cut(s) 244
Sfr303I CCGCGG 1 cut(s) 160
SgrBI CCGCGG 1 cut(s) 160
SinI GGWCC 2 cut(s) 322, 350
SlaI CTCGAG 1 cut(s) 244
SmlI CTYRAG 2 cut(s) 244, 257
SmoI CTYRAG 2 cut(s) 244, 257
SspDI GGCGCC 1 cut(s) 14
SspMI CTAG 1 cut(s) 494
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 1 cut(s) 528
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 3 cut(s) 354, 505, 553
TaiI ACGT 2 cut(s) 48, 336
TaqI TCGA 3 cut(s) 111, 195, 245
TauI GCSGC 6 cut(s) 7, 123, 162, 209, 212, 288
TfiI GAWTC 2 cut(s) 484, 563
TscAI CASTG 1 cut(s) 558
TseI GCWGC 2 cut(s) 465, 542
TspDTI ATGAA 1 cut(s) 393
TspRI CASTG 1 cut(s) 558
Tth111I GACNNNGTC 1 cut(s) 401
VpaK11BI GGWCC 2 cut(s) 322, 350
XhoI CTCGAG 1 cut(s) 244
XspI CTAG 1 cut(s) 494
ZraI GACGTC 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.