Rmu_sc0003784.1_g000019

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003784.1
OG0022225
Physical Location & Seq
Forward (+)
73793 .. 74371
579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003784.1_g000019.1.cds

Sequence Viewer

Length: 579 bp
atggcggatgatgaggacgactacttttcgtcaactagtccccatgttggaggtttccggctgcgctgggacgtatttctaagcttcagaggcgaagacacccgggaaacgatcaccaagaatctctacgaggcgctccaacgacgcggcgtacgagttttcctagacaatgacggtttggaccgcggggacgacatcggcccgactctactggaagccatcgaggactcggcggcggccatcatcgtcctgtcgccgaggtatgccgactcaagatggtgtctcaaagagcttgctaagatttgcgagggcagtaggagacttattctgcctctgttttaccaagtggatccctccgacgttcggcggcagaccggaccgtttgaggagcactttagactgcatgagcagatgtttgatagcgccacggtctctggatggaggactgccatgaccaaagtaggtaataaagctggttatcccttcagcagctcaaggtatgtaacctctgtgtgtgtgtgtcctaatctggatgtgcatattggatcgattgggaactttgtgatcaatgtcctgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

192

Amino Acids

21.7

Weight (kDa)

5.23

Isoelectric Point (pI)

53.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022225)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35421
pyrus_communis pycom16g23320
rosa_multiflora Rmu_sc0003784.1_g000019 Rmu_sc0019418.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 147, 186
AciI CCGC 7 cut(s) 5, 147, 184, 186, 233, 236, 367
AclWI GGATC 3 cut(s) 344, 357, 553
AcoI YGGCCR 1 cut(s) 237
AcuI CTGAAG 2 cut(s) 70, 469
AfaI GTAC 1 cut(s) 153
AfiI CCNNNNNNNGG 2 cut(s) 47, 363
AhlI ACTAGT 1 cut(s) 35
AluBI AGCT 4 cut(s) 84, 292, 473, 492
AluI AGCT 4 cut(s) 84, 292, 473, 492
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 3 cut(s) 287, 313, 436
AlwI GGATC 3 cut(s) 344, 357, 553
Ama87I CYCGRG 1 cut(s) 102
AoxI GGCC 2 cut(s) 199, 237
ApeKI GCWGC 2 cut(s) 61, 489
AspLEI GCGC 3 cut(s) 66, 136, 425
AspS9I GGNCC 3 cut(s) 181, 200, 377
AsuC2I CCSGG 2 cut(s) 103, 104
AsuHPI GGTGA 1 cut(s) 106
AvaI CYCGRG 1 cut(s) 102
AvaII GGWCC 2 cut(s) 181, 377
BamHI GGATCC 1 cut(s) 349
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 393
BbvI GCAGC 2 cut(s) 48, 501
BccI CCATC 4 cut(s) 227, 248, 270, 432
BclI TGATCA 1 cut(s) 564
BcnI CCSGG 2 cut(s) 103, 104
BcoDI GTCTC 3 cut(s) 287, 313, 436
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 2 cut(s) 36, 164
BfoI RGCGCY 2 cut(s) 137, 426
BisI GCNGC 6 cut(s) 62, 148, 234, 237, 368, 490
BlsI GCNGC 6 cut(s) 63, 149, 235, 238, 369, 491
Bme1390I CCNGG 2 cut(s) 103, 104
Bme18I GGWCC 2 cut(s) 181, 377
BmeT110I CYCGRG 1 cut(s) 102
BmgT120I GGNCC 3 cut(s) 181, 200, 377
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 2 cut(s) 103, 104
BpiI GAAGAC 1 cut(s) 102
BpuEI CTTGAG 2 cut(s) 256, 478
BpuMI CCSGG 2 cut(s) 103, 104
Bsa29I ATCGAT 1 cut(s) 548
BsaI GGTCTC 1 cut(s) 436
BsaJI CCNNGG 4 cut(s) 102, 184, 257, 426
BsaWI WCCGGW 1 cut(s) 374
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 363
Bse1I ACTGG 1 cut(s) 216
BseCI ATCGAT 1 cut(s) 548
BseDI CCNNGG 4 cut(s) 102, 184, 257, 426
BseGI GGATG 3 cut(s) 13, 443, 538
BseLI CCNNNNNNNGG 2 cut(s) 47, 363
BseNI ACTGG 1 cut(s) 216
BseRI GAGGAG 1 cut(s) 401
BseXI GCAGC 2 cut(s) 48, 501
BseYI CCCAGC 1 cut(s) 66
Bsh1236I CGCG 2 cut(s) 147, 186
BshFI GGCC 2 cut(s) 201, 239
BshVI ATCGAT 1 cut(s) 548
BsiHKAI GWGCWC 1 cut(s) 393
BsiHKCI CYCGRG 1 cut(s) 102
BsiSI CCGG 3 cut(s) 58, 103, 375
BsiWI CGTACG 1 cut(s) 151
BslFI GGGAC 3 cut(s) 24, 83, 203
BslI CCNNNNNNNGG 2 cut(s) 47, 363
BsmAI GTCTC 3 cut(s) 287, 313, 436
BsmFI GGGAC 3 cut(s) 24, 83, 203
BsnI GGCC 2 cut(s) 201, 239
Bso31I GGTCTC 1 cut(s) 436
BsoBI CYCGRG 1 cut(s) 102
Bsp1286I GDGCHC 1 cut(s) 393
Bsp143I GATC 4 cut(s) 111, 349, 545, 564
BspACI CCGC 7 cut(s) 5, 147, 184, 186, 233, 236, 367
BspANI GGCC 2 cut(s) 201, 239
BspDI ATCGAT 1 cut(s) 548
BspFNI CGCG 2 cut(s) 147, 186
BspLI GGNNCC 1 cut(s) 351
BspPI GGATC 3 cut(s) 344, 357, 553
BspTNI GGTCTC 1 cut(s) 436
BsrI ACTGG 1 cut(s) 216
BssECI CCNNGG 4 cut(s) 102, 184, 257, 426
BssMI GATC 4 cut(s) 111, 349, 545, 564
Bst4CI ACNGT 3 cut(s) 176, 381, 430
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 2 cut(s) 80, 297
BstDSI CCRYGG 2 cut(s) 184, 426
BstF5I GGATG 3 cut(s) 13, 443, 538
BstFNI CGCG 2 cut(s) 147, 186
BstH2I RGCGCY 2 cut(s) 137, 426
BstHHI GCGC 3 cut(s) 66, 136, 425
BstKTI GATC 4 cut(s) 114, 352, 548, 567
BstMAI GTCTC 3 cut(s) 287, 313, 436
BstMBI GATC 4 cut(s) 111, 349, 545, 564
BstMWI GCNNNNNNNGC 1 cut(s) 90
BstSCI CCNGG 2 cut(s) 101, 102
BstUI CGCG 2 cut(s) 147, 186
BstV1I GCAGC 2 cut(s) 48, 501
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
Bsu15I ATCGAT 1 cut(s) 548
BsuRI GGCC 2 cut(s) 201, 239
BsuTUI ATCGAT 1 cut(s) 548
BtgI CCRYGG 2 cut(s) 184, 426
BtsCI GGATG 3 cut(s) 13, 443, 538
Cac8I GCNNGC 1 cut(s) 294
CfoI GCGC 3 cut(s) 66, 136, 425
Cfr13I GGNCC 3 cut(s) 181, 200, 377
Cfr42I CCGCGG 1 cut(s) 187
Cfr9I CCCGGG 1 cut(s) 102
ClaI ATCGAT 1 cut(s) 548
CpoI CGGWCCG 1 cut(s) 377
CseI GACGC 1 cut(s) 153
Csp6I GTAC 1 cut(s) 152
CspI CGGWCCG 1 cut(s) 377
CviAII CATG 3 cut(s) 44, 404, 451
CviJI RGCY 8 cut(s) 61, 84, 201, 218, 239, 292, 473, 492
CviKI_1 RGCY 8 cut(s) 61, 84, 201, 218, 239, 292, 473, 492
CviQI GTAC 1 cut(s) 152
DdeI CTNAG 2 cut(s) 80, 297
DpnI GATC 4 cut(s) 113, 351, 547, 566
DpnII GATC 4 cut(s) 111, 349, 545, 564
EaeI YGGCCR 1 cut(s) 237
EciI GGCGGA 1 cut(s) 20
Eco31I GGTCTC 1 cut(s) 436
Eco47I GGWCC 2 cut(s) 181, 377
Eco57I CTGAAG 2 cut(s) 70, 469
Eco88I CYCGRG 1 cut(s) 102
FaeI CATG 3 cut(s) 47, 407, 454
FaiI YATR 6 cut(s) 45, 264, 405, 452, 501, 540
FaqI GGGAC 3 cut(s) 24, 83, 203
FatI CATG 3 cut(s) 43, 403, 450
FauI CCCGC 1 cut(s) 179
FbaI TGATCA 1 cut(s) 564
Fnu4HI GCNGC 6 cut(s) 62, 148, 234, 237, 368, 490
FokI GGATG 3 cut(s) 20, 450, 545
Fsp4HI GCNGC 6 cut(s) 62, 148, 234, 237, 368, 490
FspBI CTAG 2 cut(s) 36, 164
GlaI GCGC 3 cut(s) 65, 135, 424
GluI GCNGC 6 cut(s) 62, 148, 234, 237, 368, 490
GsaI CCCAGC 1 cut(s) 70
HaeII RGCGCY 2 cut(s) 137, 426
HaeIII GGCC 2 cut(s) 201, 239
HapII CCGG 3 cut(s) 58, 103, 375
HgaI GACGC 1 cut(s) 153
HhaI GCGC 3 cut(s) 66, 136, 425
Hin1II CATG 3 cut(s) 47, 407, 454
Hin6I GCGC 3 cut(s) 64, 134, 423
HinP1I GCGC 3 cut(s) 64, 134, 423
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HindIII AAGCTT 1 cut(s) 82
HinfI GANTC 4 cut(s) 121, 205, 227, 269
HpaII CCGG 3 cut(s) 58, 103, 375
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 2 cut(s) 89, 358
Hpy188III TCNNGA 3 cut(s) 273, 435, 530
Hpy8I GTNNAC 1 cut(s) 33
Hpy99I CGWCG 2 cut(s) 147, 362
HpyAV CCTTC 1 cut(s) 493
HpyCH4III ACNGT 3 cut(s) 176, 381, 430
HpyCH4IV ACGT 2 cut(s) 72, 360
HpyCH4V TGCA 2 cut(s) 403, 538
HpyF10VI GCNNNNNNNGC 1 cut(s) 90
HpyF3I CTNAG 2 cut(s) 80, 297
HpySE526I ACGT 2 cut(s) 72, 360
Hsp92II CATG 3 cut(s) 47, 407, 454
HspAI GCGC 3 cut(s) 64, 134, 423
Ksp22I TGATCA 1 cut(s) 564
KspI CCGCGG 1 cut(s) 187
Kzo9I GATC 4 cut(s) 111, 349, 545, 564
LmnI GCTCC 2 cut(s) 141, 388
LpnPI CCDG 9 cut(s) 52, 71, 116, 197, 263, 388, 420, 459, 515
Lsp1109I GCAGC 2 cut(s) 48, 501
MaeI CTAG 2 cut(s) 36, 164
MaeII ACGT 2 cut(s) 72, 360
MaeIII GTNAC 1 cut(s) 502
MalI GATC 4 cut(s) 113, 351, 547, 566
MboI GATC 4 cut(s) 111, 349, 545, 564
MboII GAAGA 1 cut(s) 107
MflI RGATCY 1 cut(s) 349
MhlI GDGCHC 1 cut(s) 393
MlyI GAGTC 3 cut(s) 199, 221, 263
MmeI TCCRAC 3 cut(s) 28, 163, 381
MspA1I CMGCKG 1 cut(s) 186
MspI CCGG 3 cut(s) 58, 103, 375
MspR9I CCNGG 2 cut(s) 103, 104
MvnI CGCG 2 cut(s) 147, 186
MwoI GCNNNNNNNGC 1 cut(s) 90
NciI CCSGG 2 cut(s) 103, 104
NdeII GATC 4 cut(s) 111, 349, 545, 564
NlaIII CATG 3 cut(s) 47, 407, 454
NlaIV GGNNCC 1 cut(s) 351
NmeAIII GCCGAG 2 cut(s) 209, 282
PcsI WCGNNNNNNNCGW 1 cut(s) 151
PfeI GAWTC 1 cut(s) 121
Pfl23II CGTACG 1 cut(s) 151
PkrI GCNGC 6 cut(s) 63, 149, 235, 238, 369, 491
PleI GAGTC 3 cut(s) 199, 221, 263
PpsI GAGTC 3 cut(s) 199, 221, 263
PspFI CCCAGC 1 cut(s) 66
PspLI CGTACG 1 cut(s) 151
PspN4I GGNNCC 1 cut(s) 351
PspPI GGNCC 3 cut(s) 181, 200, 377
PsuI RGATCY 1 cut(s) 349
RsaI GTAC 1 cut(s) 153
RsaNI GTAC 1 cut(s) 152
Rsr2I CGGWCCG 1 cut(s) 377
RsrII CGGWCCG 1 cut(s) 377
SacII CCGCGG 1 cut(s) 187
SatI GCNGC 6 cut(s) 62, 148, 234, 237, 368, 490
Sau3AI GATC 4 cut(s) 111, 349, 545, 564
Sau96I GGNCC 3 cut(s) 181, 200, 377
SchI GAGTC 3 cut(s) 199, 221, 263
ScrFI CCNGG 2 cut(s) 103, 104
SduI GDGCHC 1 cut(s) 393
Sfr303I CCGCGG 1 cut(s) 187
SgrBI CCGCGG 1 cut(s) 187
SinI GGWCC 2 cut(s) 181, 377
SmaI CCCGGG 1 cut(s) 104
SmlI CTYRAG 2 cut(s) 271, 493
SmoI CTYRAG 2 cut(s) 271, 493
SpeI ACTAGT 1 cut(s) 35
SsiI CCGC 7 cut(s) 5, 147, 184, 186, 233, 236, 367
SspMI CTAG 2 cut(s) 36, 164
StyD4I CCNGG 2 cut(s) 101, 102
TaaI ACNGT 3 cut(s) 176, 381, 430
TaiI ACGT 2 cut(s) 75, 363
TaqI TCGA 2 cut(s) 222, 548
TauI GCSGC 4 cut(s) 150, 236, 239, 370
TfiI GAWTC 1 cut(s) 121
TseI GCWGC 2 cut(s) 61, 489
TspMI CCCGGG 1 cut(s) 102
VpaK11BI GGWCC 2 cut(s) 181, 377
XmaI CCCGGG 1 cut(s) 102
XspI CTAG 2 cut(s) 36, 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.