Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g02340 |
| malus_domestica | MD06G1082200.v1.1 MD14G1103900.v1.1 |
| prunus_persica | Prupe.5G099500_v2.0.a1 |
| pyrus_communis | pycom14g09990 |
| rosa_chinensis | RchiOBHm_Chr7g0197791 |
| rosa_laevigata | RLG00000003907 |
| rosa_multiflora | Rmu_sc0016786.1_g000003 |
| rosa_roxburghii | Rroxscaffold_3G00258200 |
| rosa_rugosa | Rorug07G0043700 |
| rosa_samantha | Rh7CG176800 |
| rosa_wichuraiana | Rw7G014590 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 212 |
| AccB1I | GGYRCC | 1 cut(s) | 212 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 665 |
| AccI | GTMKAC | 1 cut(s) | 388 |
| AccII | CGCG | 1 cut(s) | 828 |
| AciI | CCGC | 6 cut(s) | 36, 826, 828, 906, 916, 936 |
| AcoI | YGGCCR | 3 cut(s) | 277, 922, 942 |
| AcsI | RAATTY | 1 cut(s) | 115 |
| AcyI | GRCGYC | 1 cut(s) | 885 |
| AfaI | GTAC | 5 cut(s) | 107, 149, 214, 476, 1193 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 665, 727, 920, 1077 |
| AgsI | TTSAA | 4 cut(s) | 23, 128, 542, 1124 |
| AjiI | CACGTC | 1 cut(s) | 785 |
| AjnI | CCWGG | 2 cut(s) | 208, 399 |
| AluBI | AGCT | 7 cut(s) | 188, 225, 290, 329, 334, 649, 865 |
| AluI | AGCT | 7 cut(s) | 188, 225, 290, 329, 334, 649, 865 |
| Alw21I | GWGCWC | 2 cut(s) | 867, 968 |
| Alw26I | GTCTC | 2 cut(s) | 529, 791 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 434, 533 |
| Ama87I | CYCGRG | 1 cut(s) | 727 |
| AoxI | GGCC | 5 cut(s) | 257, 277, 310, 922, 942 |
| ApeKI | GCWGC | 1 cut(s) | 714 |
| ApoI | RAATTY | 1 cut(s) | 115 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 251, 1178 |
| Asp718I | GGTACC | 1 cut(s) | 212 |
| AspS9I | GGNCC | 3 cut(s) | 199, 236, 601 |
| AsuC2I | CCSGG | 3 cut(s) | 728, 729, 1078 |
| AsuHPI | GGTGA | 3 cut(s) | 680, 844, 925 |
| AvaI | CYCGRG | 1 cut(s) | 727 |
| AvaII | GGWCC | 3 cut(s) | 199, 236, 601 |
| AxyI | CCTNAGG | 1 cut(s) | 519 |
| BalI | TGGCCA | 1 cut(s) | 279 |
| BanI | GGYRCC | 1 cut(s) | 212 |
| BanII | GRGCYC | 1 cut(s) | 867 |
| BbsI | GAAGAC | 1 cut(s) | 499 |
| Bbv12I | GWGCWC | 2 cut(s) | 867, 968 |
| BbvI | GCAGC | 1 cut(s) | 701 |
| BccI | CCATC | 3 cut(s) | 129, 863, 1318 |
| BceAI | ACGGC | 2 cut(s) | 746, 929 |
| BciT130I | CCWGG | 2 cut(s) | 210, 401 |
| BclI | TGATCA | 2 cut(s) | 91, 990 |
| BcnI | CCSGG | 3 cut(s) | 728, 729, 1078 |
| BcoDI | GTCTC | 2 cut(s) | 529, 791 |
| BfaI | CTAG | 2 cut(s) | 183, 1333 |
| BisI | GCNGC | 3 cut(s) | 715, 829, 906 |
| BlpI | GCTNAGC | 1 cut(s) | 330 |
| BlsI | GCNGC | 3 cut(s) | 716, 830, 907 |
| Bme1390I | CCNGG | 5 cut(s) | 210, 401, 728, 729, 1078 |
| Bme18I | GGWCC | 3 cut(s) | 199, 236, 601 |
| BmeT110I | CYCGRG | 1 cut(s) | 727 |
| BmgBI | CACGTC | 1 cut(s) | 785 |
| BmgT120I | GGNCC | 3 cut(s) | 199, 236, 601 |
| BmiI | GGNNCC | 4 cut(s) | 214, 445, 725, 832 |
| BmrFI | CCNGG | 5 cut(s) | 210, 401, 728, 729, 1078 |
| BmsI | GCATC | 2 cut(s) | 331, 1270 |
| BpiI | GAAGAC | 1 cut(s) | 499 |
| Bpu10I | CCTNAGC | 1 cut(s) | 861 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 330 |
| BpuEI | CTTGAG | 4 cut(s) | 152, 224, 247, 629 |
| BpuMI | CCSGG | 3 cut(s) | 728, 729, 1078 |
| Bsa29I | ATCGAT | 2 cut(s) | 339, 1118 |
| BsaHI | GRCGYC | 1 cut(s) | 885 |
| BsaI | GGTCTC | 1 cut(s) | 529 |
| BsaJI | CCNNGG | 7 cut(s) | 216, 415, 727, 826, 919, 939, 952 |
| BsaWI | WCCGGW | 1 cut(s) | 720 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 56, 86 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 665, 727, 920, 1077 |
| Bse1I | ACTGG | 1 cut(s) | 103 |
| Bse21I | CCTNAGG | 1 cut(s) | 519 |
| Bse3DI | GCAATG | 2 cut(s) | 38, 1065 |
| BseBI | CCWGG | 2 cut(s) | 210, 401 |
| BseCI | ATCGAT | 2 cut(s) | 339, 1118 |
| BseDI | CCNNGG | 7 cut(s) | 216, 415, 727, 826, 919, 939, 952 |
| BseGI | GGATG | 4 cut(s) | 771, 855, 1216, 1285 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 665, 727, 920, 1077 |
| BseMI | GCAATG | 2 cut(s) | 38, 1065 |
| BseMII | CTCAG | 6 cut(s) | 321, 516, 540, 852, 1218, 1224 |
| BseNI | ACTGG | 1 cut(s) | 103 |
| BseX3I | CGGCCG | 1 cut(s) | 942 |
| BseXI | GCAGC | 1 cut(s) | 701 |
| BseYI | CCCAGC | 1 cut(s) | 290 |
| Bsh1236I | CGCG | 1 cut(s) | 828 |
| Bsh1285I | CGRYCG | 1 cut(s) | 945 |
| BshFI | GGCC | 5 cut(s) | 259, 279, 312, 924, 944 |
| BshNI | GGYRCC | 1 cut(s) | 212 |
| BshVI | ATCGAT | 2 cut(s) | 339, 1118 |
| BsiEI | CGRYCG | 1 cut(s) | 945 |
| BsiHKAI | GWGCWC | 2 cut(s) | 867, 968 |
| BsiHKCI | CYCGRG | 1 cut(s) | 727 |
| BsiSI | CCGG | 3 cut(s) | 721, 728, 1077 |
| BslFI | GGGAC | 2 cut(s) | 744, 896 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 665, 727, 920, 1077 |
| BsmAI | GTCTC | 2 cut(s) | 529, 791 |
| BsmBI | CGTCTC | 1 cut(s) | 791 |
| BsmFI | GGGAC | 2 cut(s) | 744, 896 |
| BsnI | GGCC | 5 cut(s) | 259, 279, 312, 924, 944 |
| Bso31I | GGTCTC | 1 cut(s) | 529 |
| BsoBI | CYCGRG | 1 cut(s) | 727 |
| Bsp1286I | GDGCHC | 2 cut(s) | 867, 968 |
| Bsp143I | GATC | 6 cut(s) | 91, 336, 462, 670, 684, 990 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 330 |
| Bsp19I | CCATGG | 2 cut(s) | 216, 919 |
| BspACI | CCGC | 6 cut(s) | 36, 826, 828, 906, 916, 936 |
| BspANI | GGCC | 5 cut(s) | 259, 279, 312, 924, 944 |
| BspCNI | CTCAG | 6 cut(s) | 322, 515, 539, 853, 1219, 1225 |
| BspDI | ATCGAT | 2 cut(s) | 339, 1118 |
| BspFNI | CGCG | 1 cut(s) | 828 |
| BspLI | GGNNCC | 4 cut(s) | 214, 445, 725, 832 |
| BspT107I | GGYRCC | 1 cut(s) | 212 |
| BspTNI | GGTCTC | 1 cut(s) | 529 |
| BsrDI | GCAATG | 2 cut(s) | 38, 1065 |
| BsrI | ACTGG | 1 cut(s) | 103 |
| BssECI | CCNNGG | 7 cut(s) | 216, 415, 727, 826, 919, 939, 952 |
| BssMI | GATC | 6 cut(s) | 91, 336, 462, 670, 684, 990 |
| BssNI | GRCGYC | 1 cut(s) | 885 |
| BssT1I | CCWWGG | 3 cut(s) | 216, 415, 919 |
| Bst2UI | CCWGG | 2 cut(s) | 210, 401 |
| Bst4CI | ACNGT | 3 cut(s) | 535, 845, 1159 |
| Bst6I | CTCTTC | 3 cut(s) | 465, 1299, 1314 |
| BstACI | GRCGYC | 1 cut(s) | 885 |
| BstDEI | CTNAG | 7 cut(s) | 330, 502, 519, 526, 861, 1227, 1233 |
| BstDSI | CCRYGG | 4 cut(s) | 216, 826, 919, 952 |
| BstEII | GGTNACC | 1 cut(s) | 38 |
| BstF5I | GGATG | 4 cut(s) | 771, 855, 1216, 1285 |
| BstFNI | CGCG | 1 cut(s) | 828 |
| BstKTI | GATC | 6 cut(s) | 94, 339, 465, 673, 687, 993 |
| BstMAI | GTCTC | 2 cut(s) | 529, 791 |
| BstMBI | GATC | 6 cut(s) | 91, 336, 462, 670, 684, 990 |
| BstMCI | CGRYCG | 1 cut(s) | 945 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 963 |
| BstNI | CCWGG | 2 cut(s) | 210, 401 |
| BstNSI | RCATGY | 1 cut(s) | 714 |
| BstPI | GGTNACC | 1 cut(s) | 38 |
| BstSCI | CCNGG | 5 cut(s) | 208, 399, 726, 727, 1076 |
| BstUI | CGCG | 1 cut(s) | 828 |
| BstV1I | GCAGC | 1 cut(s) | 701 |
| BstV2I | GAAGAC | 1 cut(s) | 499 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 416, 1102 |
| BstZI | CGGCCG | 1 cut(s) | 942 |
| Bsu15I | ATCGAT | 2 cut(s) | 339, 1118 |
| Bsu36I | CCTNAGG | 1 cut(s) | 519 |
| BsuRI | GGCC | 5 cut(s) | 259, 279, 312, 924, 944 |
| BsuTUI | ATCGAT | 2 cut(s) | 339, 1118 |
| BtgI | CCRYGG | 4 cut(s) | 216, 826, 919, 952 |
| BtrI | CACGTC | 1 cut(s) | 785 |
| BtsCI | GGATG | 4 cut(s) | 771, 855, 1216, 1285 |
| BtsIMutI | CAGTG | 3 cut(s) | 531, 1155, 1224 |
| CaiI | CAGNNNCTG | 2 cut(s) | 434, 533 |
| Cfr13I | GGNCC | 3 cut(s) | 199, 236, 601 |
| Cfr42I | CCGCGG | 1 cut(s) | 829 |
| Cfr9I | CCCGGG | 1 cut(s) | 727 |
| ClaI | ATCGAT | 2 cut(s) | 339, 1118 |
| CseI | GACGC | 1 cut(s) | 893 |
| Csp6I | GTAC | 5 cut(s) | 106, 148, 213, 475, 1192 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 860, 895 |
| CviQI | GTAC | 5 cut(s) | 106, 148, 213, 475, 1192 |
| DdeI | CTNAG | 7 cut(s) | 330, 502, 519, 526, 861, 1227, 1233 |
| DpnI | GATC | 6 cut(s) | 93, 338, 464, 672, 686, 992 |
| DpnII | GATC | 6 cut(s) | 91, 336, 462, 670, 684, 990 |
| EaeI | YGGCCR | 3 cut(s) | 277, 922, 942 |
| EagI | CGGCCG | 1 cut(s) | 942 |
| Eam1104I | CTCTTC | 3 cut(s) | 465, 1299, 1314 |
| EarI | CTCTTC | 3 cut(s) | 465, 1299, 1314 |
| Ecl136II | GAGCTC | 1 cut(s) | 865 |
| EclXI | CGGCCG | 1 cut(s) | 942 |
| Eco130I | CCWWGG | 3 cut(s) | 216, 415, 919 |
| Eco24I | GRGCYC | 1 cut(s) | 867 |
| Eco31I | GGTCTC | 1 cut(s) | 529 |
| Eco32I | GATATC | 2 cut(s) | 456, 628 |
| Eco47I | GGWCC | 3 cut(s) | 199, 236, 601 |
| Eco52I | CGGCCG | 1 cut(s) | 942 |
| Eco53kI | GAGCTC | 1 cut(s) | 865 |
| Eco81I | CCTNAGG | 1 cut(s) | 519 |
| Eco88I | CYCGRG | 1 cut(s) | 727 |
| Eco91I | GGTNACC | 1 cut(s) | 38 |
| EcoICRI | GAGCTC | 1 cut(s) | 865 |
| EcoO109I | RGGNCCY | 1 cut(s) | 236 |
| EcoO65I | GGTNACC | 1 cut(s) | 38 |
| EcoRI | GAATTC | 1 cut(s) | 115 |
| EcoRII | CCWGG | 2 cut(s) | 208, 399 |
| EcoRV | GATATC | 2 cut(s) | 456, 628 |
| EcoT14I | CCWWGG | 3 cut(s) | 216, 415, 919 |
| EcoT38I | GRGCYC | 1 cut(s) | 867 |
| ErhI | CCWWGG | 3 cut(s) | 216, 415, 919 |
| Esp3I | CGTCTC | 1 cut(s) | 791 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1166, 1198 |
| FaqI | GGGAC | 2 cut(s) | 744, 896 |
| FauI | CCCGC | 2 cut(s) | 833, 923 |
| FbaI | TGATCA | 2 cut(s) | 91, 990 |
| FblI | GTMKAC | 1 cut(s) | 388 |
| Fnu4HI | GCNGC | 3 cut(s) | 715, 829, 906 |
| FokI | GGATG | 4 cut(s) | 758, 842, 1223, 1292 |
| FriOI | GRGCYC | 1 cut(s) | 867 |
| Fsp4HI | GCNGC | 3 cut(s) | 715, 829, 906 |
| FspBI | CTAG | 2 cut(s) | 183, 1333 |
| GluI | GCNGC | 3 cut(s) | 715, 829, 906 |
| GsaI | CCCAGC | 1 cut(s) | 294 |
| HaeIII | GGCC | 5 cut(s) | 259, 279, 312, 924, 944 |
| HapII | CCGG | 3 cut(s) | 721, 728, 1077 |
| HgaI | GACGC | 1 cut(s) | 893 |
| Hin1I | GRCGYC | 1 cut(s) | 885 |
| HinfI | GANTC | 5 cut(s) | 138, 705, 766, 1033, 1120 |
| HpaII | CCGG | 3 cut(s) | 721, 728, 1077 |
| HphI | GGTGA | 3 cut(s) | 680, 844, 925 |
| Hpy166II | GTNNAC | 4 cut(s) | 148, 389, 1194, 1224 |
| Hpy188I | TCNGA | 9 cut(s) | 50, 453, 529, 638, 675, 745, 752, 1147, 1261 |
| Hpy188III | TCNNGA | 4 cut(s) | 350, 435, 1124, 1277 |
| Hpy8I | GTNNAC | 4 cut(s) | 148, 389, 1194, 1224 |
| HpyAV | CCTTC | 5 cut(s) | 317, 458, 522, 1168, 1196 |
| HpyCH4III | ACNGT | 3 cut(s) | 535, 845, 1159 |
| HpyCH4IV | ACGT | 3 cut(s) | 18, 108, 784 |
| HpyCH4V | TGCA | 3 cut(s) | 16, 597, 1186 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 963 |
| HpyF3I | CTNAG | 7 cut(s) | 330, 502, 519, 526, 861, 1227, 1233 |
| HpySE526I | ACGT | 3 cut(s) | 18, 108, 784 |
| Hsp92I | GRCGYC | 1 cut(s) | 885 |
| KpnI | GGTACC | 1 cut(s) | 216 |
| Ksp22I | TGATCA | 2 cut(s) | 91, 990 |
| KspI | CCGCGG | 1 cut(s) | 829 |
| Kzo9I | GATC | 6 cut(s) | 91, 336, 462, 670, 684, 990 |
| LmnI | GCTCC | 3 cut(s) | 287, 326, 836 |
| Lsp1109I | GCAGC | 1 cut(s) | 701 |
| LweI | GCATC | 2 cut(s) | 331, 1270 |
| MaeI | CTAG | 2 cut(s) | 183, 1333 |
| MaeII | ACGT | 3 cut(s) | 18, 108, 784 |
| MaeIII | GTNAC | 4 cut(s) | 38, 706, 1061, 1238 |
| MalI | GATC | 6 cut(s) | 93, 338, 464, 672, 686, 992 |
| MboI | GATC | 6 cut(s) | 91, 336, 462, 670, 684, 990 |
| MboII | GAAGA | 6 cut(s) | 116, 482, 499, 787, 1316, 1331 |
| MfeI | CAATTG | 1 cut(s) | 298 |
| MhlI | GDGCHC | 2 cut(s) | 867, 968 |
| MlsI | TGGCCA | 1 cut(s) | 279 |
| MluCI | AATT | 6 cut(s) | 87, 115, 298, 376, 572, 1151 |
| MluNI | TGGCCA | 1 cut(s) | 279 |
| MlyI | GAGTC | 2 cut(s) | 147, 714 |
| MmeI | TCCRAC | 1 cut(s) | 768 |
| Mox20I | TGGCCA | 1 cut(s) | 279 |
| MroXI | GAANNNNTTC | 2 cut(s) | 251, 1178 |
| MscI | TGGCCA | 1 cut(s) | 279 |
| MslI | CAYNNNNRTG | 3 cut(s) | 695, 1100, 1160 |
| Msp20I | TGGCCA | 1 cut(s) | 279 |
| MspA1I | CMGCKG | 1 cut(s) | 828 |
| MspI | CCGG | 3 cut(s) | 721, 728, 1077 |
| MspR9I | CCNGG | 5 cut(s) | 210, 401, 728, 729, 1078 |
| MunI | CAATTG | 1 cut(s) | 298 |
| MvaI | CCWGG | 2 cut(s) | 210, 401 |
| MvnI | CGCG | 1 cut(s) | 828 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 963 |
| NciI | CCSGG | 3 cut(s) | 728, 729, 1078 |
| NcoI | CCATGG | 2 cut(s) | 216, 919 |
| NdeII | GATC | 6 cut(s) | 91, 336, 462, 670, 684, 990 |
| NlaIV | GGNNCC | 4 cut(s) | 214, 445, 725, 832 |
| NmeAIII | GCCGAG | 1 cut(s) | 920 |
| NmuCI | GTSAC | 2 cut(s) | 706, 1061 |
| NspI | RCATGY | 1 cut(s) | 714 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 585 |
| PdmI | GAANNNNTTC | 2 cut(s) | 251, 1178 |
| PfeI | GAWTC | 3 cut(s) | 766, 1033, 1120 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 665 |
| PkrI | GCNGC | 3 cut(s) | 716, 830, 907 |
| PleI | GAGTC | 2 cut(s) | 146, 713 |
| PpsI | GAGTC | 2 cut(s) | 146, 713 |
| PpuMI | RGGWCCY | 1 cut(s) | 236 |
| Psp124BI | GAGCTC | 1 cut(s) | 867 |
| Psp5II | RGGWCCY | 1 cut(s) | 236 |
| Psp6I | CCWGG | 2 cut(s) | 208, 399 |
| PspEI | GGTNACC | 1 cut(s) | 38 |
| PspFI | CCCAGC | 1 cut(s) | 290 |
| PspGI | CCWGG | 2 cut(s) | 208, 399 |
| PspN4I | GGNNCC | 4 cut(s) | 214, 445, 725, 832 |
| PspPI | GGNCC | 3 cut(s) | 199, 236, 601 |
| PspPPI | RGGWCCY | 1 cut(s) | 236 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1001, 1033 |
| PstNI | CAGNNNCTG | 2 cut(s) | 434, 533 |
| RsaI | GTAC | 5 cut(s) | 107, 149, 214, 476, 1193 |
| RsaNI | GTAC | 5 cut(s) | 106, 148, 213, 475, 1192 |
| RseI | CAYNNNNRTG | 3 cut(s) | 695, 1100, 1160 |
| SacI | GAGCTC | 1 cut(s) | 867 |
| SacII | CCGCGG | 1 cut(s) | 829 |
| SatI | GCNGC | 3 cut(s) | 715, 829, 906 |
| Sau3AI | GATC | 6 cut(s) | 91, 336, 462, 670, 684, 990 |
| Sau96I | GGNCC | 3 cut(s) | 199, 236, 601 |
| SchI | GAGTC | 2 cut(s) | 147, 714 |
| ScrFI | CCNGG | 5 cut(s) | 210, 401, 728, 729, 1078 |
| SduI | GDGCHC | 2 cut(s) | 867, 968 |
| SfaNI | GCATC | 2 cut(s) | 331, 1270 |
| Sfr303I | CCGCGG | 1 cut(s) | 829 |
| SgrBI | CCGCGG | 1 cut(s) | 829 |
| SinI | GGWCC | 3 cut(s) | 199, 236, 601 |
| SmaI | CCCGGG | 1 cut(s) | 729 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 695, 1100, 1160 |
| SmlI | CTYRAG | 4 cut(s) | 131, 203, 226, 644 |
| SmoI | CTYRAG | 4 cut(s) | 131, 203, 226, 644 |
| Sse9I | AATT | 6 cut(s) | 87, 115, 298, 376, 572, 1151 |
| SsiI | CCGC | 6 cut(s) | 36, 826, 828, 906, 916, 936 |
| SspMI | CTAG | 2 cut(s) | 183, 1333 |
| SstI | GAGCTC | 1 cut(s) | 867 |
| StyD4I | CCNGG | 5 cut(s) | 208, 399, 726, 727, 1076 |
| StyI | CCWWGG | 3 cut(s) | 216, 415, 919 |
| TaaI | ACNGT | 3 cut(s) | 535, 845, 1159 |
| TaiI | ACGT | 3 cut(s) | 21, 111, 787 |
| TaqI | TCGA | 5 cut(s) | 172, 339, 579, 1112, 1118 |
| TasI | AATT | 6 cut(s) | 87, 115, 298, 376, 572, 1151 |
| TatI | WGTACW | 1 cut(s) | 147 |
| TauI | GCSGC | 2 cut(s) | 831, 908 |
| TfiI | GAWTC | 3 cut(s) | 766, 1033, 1120 |
| TscAI | CASTG | 3 cut(s) | 538, 1162, 1231 |
| TseFI | GTSAC | 2 cut(s) | 706, 1061 |
| TseI | GCWGC | 1 cut(s) | 714 |
| Tsp45I | GTSAC | 2 cut(s) | 706, 1061 |
| TspDTI | ATGAA | 7 cut(s) | 557, 627, 787, 996, 1090, 1227, 1259 |
| TspGWI | ACGGA | 1 cut(s) | 941 |
| TspMI | CCCGGG | 1 cut(s) | 727 |
| TspRI | CASTG | 3 cut(s) | 538, 1162, 1231 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 665 |
| VpaK11BI | GGWCC | 3 cut(s) | 199, 236, 601 |
| XapI | RAATTY | 1 cut(s) | 115 |
| XceI | RCATGY | 1 cut(s) | 714 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 287 |
| XmaI | CCCGGG | 1 cut(s) | 727 |
| XmiI | GTMKAC | 1 cut(s) | 388 |
| XmnI | GAANNNNTTC | 2 cut(s) | 251, 1178 |
| XspI | CTAG | 2 cut(s) | 183, 1333 |