MD06G1082200.v1.1

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0017484
Physical Location & Seq
Reverse (-)
20163869 .. 20166271
2403 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1082200.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGAGAGAAATCTTGCACATTCAAGCTGGACAATGTGGGAACCAAATTGGAGGGAAGTTTTGGGAGGTGATGTGTGACGAGCATGGGATCGATCCTACAGGAAAATATAACGGGAACTCCCACCTTCAGCTTGAGAGGGTAAATGTTTACTACAATGAAGCAAATGGAGGGAGATATGTGCCTAGGGCAGTGCTGATGGACCTCGAGCCGGGGACAATGGACAGCTTAAGAACTGGTCCTTATGGGCAGATTTTCAGGCCGGACAACTTTGTGTTCGGCCAGAATGGAGCTGGAAACAATTGGGCTAAAGGGCATTACACAGAAGGAGCTGAATTGATTGATGCTGTTCTTGATGTGGTTAGAAAAGAGGCTGAGAATTGTGATTGTTTACAAGGGTTCCAGATTTGTCATTCACTAGGAGGTGGGACAGGATCAGGAATGGGGACCCTACTGATTTCAAAGATCAGGGAAGAGTACCCAGATAGAATGATGATGACTTTCTCAGTCTTCCCCTCACCAAAAGTCTCAGACACAGTGGTCGAACCCTACAATGCAACCCTCTCTGTTCACCAATTGGTTGAAAATGCTGATGAGGTCATGGTCCTTGACAATGAAGCCCTCTATGATATTTGTTTCAGAACTCTCAAGCTCACAAATCCAAGAGTTGGTGATTTGAACCATTTGATTTCGACAACCATGAGTGGAGTCACATGTTGCCTCCGCTTCCCGGGACAGCTCAACTCCGATCTTCGAAAACTAGCCGTGAATCTCATCCCCTTCCCCCGCCTCCATTTCTTCATGGTTGGTTTCGCACCCTTGACCTCCCGCGGCTCCCAACTATATAGAGCCCTCACAATCCCTGAGCTCACACAACAAATGTGGGACGCCAAGAACATGATGTGCGCCGCAGATCCACGCCATGGTCGATACCTGACAGCATCGGCTGTGTTCCGCGGCAAAATGAGCACAAAAGAAGTGGACGAGCAGATGATCAACATACAAAACAAGAACTCCTCATTCTTTGTGGAGTGGATTCCAAACAATGTGAAATCAAGTGTTTGTGACATTCCACCAACCGGGCTTGCCATGTCCTCTACATTCATGGGGAATTCGACATCGATTCAAGAGATGTTTAGGCGGGTTTCGGAGCAGTTCACGGTCATGTTTAGGAGGAAGGCCTTCTTGCATTGGTACACAGGCGAGGGTATGGATGAGATGGAGTTTACTGAGGCTGAGAGCAACATGAATGACTTGGTCTCTGAGTATCAGCAGTACCAAGATGCTGTGGCACAGGATGAGGGAGAGTATTATGAGGAAGAGCTGGAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

444

Amino Acids

49.98

Weight (kDa)

4.95

Isoelectric Point (pI)

30.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 3 - 211 1.5e-65 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 261 - 382 7e-39 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017484)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g02340
malus_domestica MD06G1082200.v1.1 MD14G1103900.v1.1
prunus_persica Prupe.5G099500_v2.0.a1
pyrus_communis pycom14g09990
rosa_chinensis RchiOBHm_Chr7g0197791
rosa_laevigata RLG00000003907
rosa_multiflora Rmu_sc0016786.1_g000003
rosa_roxburghii Rroxscaffold_3G00258200
rosa_rugosa Rorug07G0043700
rosa_samantha Rh7CG176800
rosa_wichuraiana Rw7G014590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 536
AccB7I CCANNNNNTGG 2 cut(s) 665, 920
AccII CGCG 2 cut(s) 828, 954
AciI CCGC 8 cut(s) 721, 784, 826, 828, 906, 952, 954, 1138
AclWI GGATC 4 cut(s) 86, 95, 439, 905
AcoI YGGCCR 1 cut(s) 277
AcsI RAATTY 1 cut(s) 1108
AcuI CTGAAG 1 cut(s) 110
AcyI GRCGYC 1 cut(s) 885
AfaI GTAC 3 cut(s) 476, 1193, 1274
AfiI CCNNNNNNNGG 5 cut(s) 208, 665, 727, 920, 1076
AflII CTTAAG 1 cut(s) 226
AflIII ACRYGT 1 cut(s) 710
AgsI TTSAA 5 cut(s) 23, 459, 581, 676, 1124
AluBI AGCT 9 cut(s) 26, 130, 225, 290, 329, 649, 736, 865, 1321
AluI AGCT 9 cut(s) 26, 130, 225, 290, 329, 649, 736, 865, 1321
Alw21I GWGCWC 2 cut(s) 867, 968
Alw26I GTCTC 2 cut(s) 529, 1261
AlwI GGATC 4 cut(s) 86, 95, 439, 905
Ama87I CYCGRG 2 cut(s) 203, 727
AoxI GGCC 3 cut(s) 257, 277, 1176
ApoI RAATTY 1 cut(s) 1108
Asp700I GAANNNNTTC 1 cut(s) 1178
AspA2I CCTAGG 1 cut(s) 182
AspLEI GCGC 1 cut(s) 905
AspS9I GGNCC 4 cut(s) 199, 236, 444, 601
AsuC2I CCSGG 4 cut(s) 210, 728, 729, 1078
AsuHPI GGTGA 4 cut(s) 79, 507, 560, 680
AsuII TTCGAA 1 cut(s) 751
AvaI CYCGRG 2 cut(s) 203, 727
AvaII GGWCC 4 cut(s) 199, 236, 444, 601
AvrII CCTAGG 1 cut(s) 182
BanII GRGCYC 2 cut(s) 850, 867
BbsI GAAGAC 1 cut(s) 499
Bbv12I GWGCWC 2 cut(s) 867, 968
BccI CCATC 2 cut(s) 190, 1210
BceAI ACGGC 1 cut(s) 746
BclI TGATCA 1 cut(s) 990
BcnI CCSGG 4 cut(s) 210, 728, 729, 1078
BcoDI GTCTC 2 cut(s) 529, 1261
BfaI CTAG 3 cut(s) 183, 416, 758
BfmI CTRYAG 1 cut(s) 96
BfrI CTTAAG 1 cut(s) 226
BisI GCNGC 3 cut(s) 829, 906, 955
BlnI CCTAGG 1 cut(s) 182
BlsI GCNGC 3 cut(s) 830, 907, 956
Bme1390I CCNGG 4 cut(s) 210, 728, 729, 1078
Bme18I GGWCC 4 cut(s) 199, 236, 444, 601
BmeT110I CYCGRG 2 cut(s) 203, 727
BmgT120I GGNCC 4 cut(s) 199, 236, 444, 601
BmiI GGNNCC 5 cut(s) 41, 398, 445, 446, 832
BmrFI CCNGG 4 cut(s) 210, 728, 729, 1078
BmsI GCATC 3 cut(s) 331, 947, 1270
BpiI GAAGAC 1 cut(s) 499
Bpu10I CCTNAGC 1 cut(s) 861
Bpu14I TTCGAA 1 cut(s) 751
BpuEI CTTGAG 2 cut(s) 152, 629
BpuMI CCSGG 4 cut(s) 210, 728, 729, 1078
Bsa29I ATCGAT 2 cut(s) 90, 1118
BsaHI GRCGYC 1 cut(s) 885
BsaI GGTCTC 1 cut(s) 1261
BsaJI CCNNGG 6 cut(s) 182, 209, 727, 826, 919, 952
BsaXI ACNNNNNCTCC 6 cut(s) 56, 86, 101, 131, 995, 1025
Bsc4I CCNNNNNNNGG 5 cut(s) 208, 665, 727, 920, 1076
Bse1I ACTGG 1 cut(s) 238
BseCI ATCGAT 2 cut(s) 90, 1118
BseDI CCNNGG 6 cut(s) 182, 209, 727, 826, 919, 952
BseGI GGATG 3 cut(s) 771, 1216, 1300
BseLI CCNNNNNNNGG 5 cut(s) 208, 665, 727, 920, 1076
BseMII CTCAG 7 cut(s) 363, 516, 540, 852, 1218, 1224, 1251
BseNI ACTGG 1 cut(s) 238
BseRI GAGGAG 1 cut(s) 1003
Bsh1236I CGCG 2 cut(s) 828, 954
BshFI GGCC 3 cut(s) 259, 279, 1178
BshVI ATCGAT 2 cut(s) 90, 1118
BsiHKAI GWGCWC 2 cut(s) 867, 968
BsiHKCI CYCGRG 2 cut(s) 203, 727
BsiSI CCGG 4 cut(s) 209, 260, 728, 1077
BslFI GGGAC 5 cut(s) 226, 439, 457, 744, 896
BslI CCNNNNNNNGG 5 cut(s) 208, 665, 727, 920, 1076
BsmAI GTCTC 2 cut(s) 529, 1261
BsmFI GGGAC 5 cut(s) 226, 439, 457, 744, 896
BsnI GGCC 3 cut(s) 259, 279, 1178
Bso31I GGTCTC 1 cut(s) 1261
BsoBI CYCGRG 2 cut(s) 203, 727
Bsp119I TTCGAA 1 cut(s) 751
Bsp1286I GDGCHC 3 cut(s) 850, 867, 968
Bsp143I GATC 7 cut(s) 87, 91, 431, 462, 745, 910, 990
Bsp19I CCATGG 1 cut(s) 919
BspACI CCGC 8 cut(s) 721, 784, 826, 828, 906, 952, 954, 1138
BspANI GGCC 3 cut(s) 259, 279, 1178
BspCNI CTCAG 7 cut(s) 364, 515, 539, 853, 1219, 1225, 1252
BspDI ATCGAT 2 cut(s) 90, 1118
BspFNI CGCG 2 cut(s) 828, 954
BspLI GGNNCC 5 cut(s) 41, 398, 445, 446, 832
BspPI GGATC 4 cut(s) 86, 95, 439, 905
BspQI GCTCTTC 1 cut(s) 1311
BspT104I TTCGAA 1 cut(s) 751
BspTI CTTAAG 1 cut(s) 226
BspTNI GGTCTC 1 cut(s) 1261
BsrI ACTGG 1 cut(s) 238
BssECI CCNNGG 6 cut(s) 182, 209, 727, 826, 919, 952
BssMI GATC 7 cut(s) 87, 91, 431, 462, 745, 910, 990
BssNI GRCGYC 1 cut(s) 885
BssT1I CCWWGG 2 cut(s) 182, 919
Bst4CI ACNGT 2 cut(s) 535, 1159
Bst6I CTCTTC 2 cut(s) 465, 1311
BstACI GRCGYC 1 cut(s) 885
BstAFI CTTAAG 1 cut(s) 226
BstBI TTCGAA 1 cut(s) 751
BstC8I GCNNGC 1 cut(s) 1083
BstDEI CTNAG 7 cut(s) 372, 502, 526, 861, 1227, 1233, 1260
BstDSI CCRYGG 3 cut(s) 826, 919, 952
BstF5I GGATG 3 cut(s) 771, 1216, 1300
BstFNI CGCG 2 cut(s) 828, 954
BstHHI GCGC 1 cut(s) 905
BstKTI GATC 7 cut(s) 90, 94, 434, 465, 748, 913, 993
BstMAI GTCTC 2 cut(s) 529, 1261
BstMBI GATC 7 cut(s) 87, 91, 431, 462, 745, 910, 990
BstMWI GCNNNNNNNGC 1 cut(s) 963
BstNSI RCATGY 1 cut(s) 714
BstSCI CCNGG 4 cut(s) 208, 726, 727, 1076
BstSFI CTRYAG 1 cut(s) 96
BstUI CGCG 2 cut(s) 828, 954
BstV2I GAAGAC 1 cut(s) 499
BstX2I RGATCY 1 cut(s) 910
BstYI RGATCY 1 cut(s) 910
Bsu15I ATCGAT 2 cut(s) 90, 1118
BsuRI GGCC 3 cut(s) 259, 279, 1178
BsuTUI ATCGAT 2 cut(s) 90, 1118
BtgI CCRYGG 3 cut(s) 826, 919, 952
BtsCI GGATG 3 cut(s) 771, 1216, 1300
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 195, 540
Cac8I GCNNGC 1 cut(s) 1083
CfoI GCGC 1 cut(s) 905
Cfr13I GGNCC 4 cut(s) 199, 236, 444, 601
Cfr42I CCGCGG 2 cut(s) 829, 955
Cfr9I CCCGGG 1 cut(s) 727
ClaI ATCGAT 2 cut(s) 90, 1118
CseI GACGC 1 cut(s) 893
Csp6I GTAC 3 cut(s) 475, 1192, 1273
CspCI CAANNNNNGTGG 4 cut(s) 860, 895, 957, 992
CviQI GTAC 3 cut(s) 475, 1192, 1273
DdeI CTNAG 7 cut(s) 372, 502, 526, 861, 1227, 1233, 1260
DpnI GATC 7 cut(s) 89, 93, 433, 464, 747, 912, 992
DpnII GATC 7 cut(s) 87, 91, 431, 462, 745, 910, 990
DrdI GACNNNNNNGTC 1 cut(s) 536
DseDI GACNNNNNNGTC 1 cut(s) 536
EaeI YGGCCR 1 cut(s) 277
Eam1104I CTCTTC 2 cut(s) 465, 1311
EarI CTCTTC 2 cut(s) 465, 1311
Ecl136II GAGCTC 1 cut(s) 865
Eco130I CCWWGG 2 cut(s) 182, 919
Eco147I AGGCCT 1 cut(s) 1178
Eco24I GRGCYC 2 cut(s) 850, 867
Eco31I GGTCTC 1 cut(s) 1261
Eco47I GGWCC 4 cut(s) 199, 236, 444, 601
Eco53kI GAGCTC 1 cut(s) 865
Eco57I CTGAAG 1 cut(s) 110
Eco88I CYCGRG 2 cut(s) 203, 727
EcoICRI GAGCTC 1 cut(s) 865
EcoO109I RGGNCCY 1 cut(s) 444
EcoRI GAATTC 1 cut(s) 1108
EcoT14I CCWWGG 2 cut(s) 182, 919
EcoT38I GRGCYC 2 cut(s) 850, 867
ErhI CCWWGG 2 cut(s) 182, 919
FalI AAGNNNNNCTT 2 cut(s) 1166, 1198
FaqI GGGAC 5 cut(s) 226, 439, 457, 744, 896
FauI CCCGC 3 cut(s) 791, 833, 1131
FbaI TGATCA 1 cut(s) 990
Fnu4HI GCNGC 3 cut(s) 829, 906, 955
FokI GGATG 3 cut(s) 758, 1223, 1307
FriOI GRGCYC 2 cut(s) 850, 867
Fsp4HI GCNGC 3 cut(s) 829, 906, 955
FspBI CTAG 3 cut(s) 183, 416, 758
GlaI GCGC 1 cut(s) 904
GluI GCNGC 3 cut(s) 829, 906, 955
HaeIII GGCC 3 cut(s) 259, 279, 1178
HapII CCGG 4 cut(s) 209, 260, 728, 1077
HgaI GACGC 1 cut(s) 893
HhaI GCGC 1 cut(s) 905
Hin1I GRCGYC 1 cut(s) 885
Hin6I GCGC 1 cut(s) 903
HinP1I GCGC 1 cut(s) 903
HinfI GANTC 4 cut(s) 705, 766, 1033, 1120
HpaII CCGG 4 cut(s) 209, 260, 728, 1077
HphI GGTGA 4 cut(s) 79, 507, 560, 680
Hpy166II GTNNAC 7 cut(s) 148, 389, 568, 979, 1155, 1194, 1224
Hpy188I TCNGA 5 cut(s) 529, 638, 745, 1147, 1261
Hpy188III TCNNGA 4 cut(s) 350, 400, 435, 1124
Hpy8I GTNNAC 7 cut(s) 148, 389, 568, 979, 1155, 1194, 1224
HpyAV CCTTC 5 cut(s) 134, 317, 787, 1168, 1189
HpyCH4III ACNGT 2 cut(s) 535, 1159
HpyCH4V TGCA 3 cut(s) 16, 554, 1186
HpyF10VI GCNNNNNNNGC 1 cut(s) 963
HpyF3I CTNAG 7 cut(s) 372, 502, 526, 861, 1227, 1233, 1260
Hsp92I GRCGYC 1 cut(s) 885
HspAI GCGC 1 cut(s) 903
KflI GGGWCCC 1 cut(s) 444
Ksp22I TGATCA 1 cut(s) 990
KspI CCGCGG 2 cut(s) 829, 955
Kzo9I GATC 7 cut(s) 87, 91, 431, 462, 745, 910, 990
LguI GCTCTTC 1 cut(s) 1311
LmnI GCTCC 4 cut(s) 287, 326, 836, 1147
LweI GCATC 3 cut(s) 331, 947, 1270
MaeI CTAG 3 cut(s) 183, 416, 758
MaeIII GTNAC 3 cut(s) 74, 706, 1061
MalI GATC 7 cut(s) 89, 93, 433, 464, 747, 912, 992
MboI GATC 7 cut(s) 87, 91, 431, 462, 745, 910, 990
MboII GAAGA 5 cut(s) 482, 499, 740, 787, 1328
MfeI CAATTG 2 cut(s) 298, 572
MflI RGATCY 1 cut(s) 910
MhlI GDGCHC 3 cut(s) 850, 867, 968
MluCI AATT 6 cut(s) 45, 298, 332, 376, 572, 1108
MlyI GAGTC 1 cut(s) 714
MroXI GAANNNNTTC 1 cut(s) 1178
MseI TTAA 1 cut(s) 227
MslI CAYNNNNRTG 1 cut(s) 1160
MspA1I CMGCKG 2 cut(s) 828, 954
MspCI CTTAAG 1 cut(s) 226
MspI CCGG 4 cut(s) 209, 260, 728, 1077
MspR9I CCNGG 4 cut(s) 210, 728, 729, 1078
MunI CAATTG 2 cut(s) 298, 572
MvnI CGCG 2 cut(s) 828, 954
MwoI GCNNNNNNNGC 1 cut(s) 963
NciI CCSGG 4 cut(s) 210, 728, 729, 1078
NcoI CCATGG 1 cut(s) 919
NdeII GATC 7 cut(s) 87, 91, 431, 462, 745, 910, 990
NlaIV GGNNCC 5 cut(s) 41, 398, 445, 446, 832
NmuCI GTSAC 3 cut(s) 74, 706, 1061
NspI RCATGY 1 cut(s) 714
NspV TTCGAA 1 cut(s) 751
PaeR7I CTCGAG 1 cut(s) 203
PceI AGGCCT 1 cut(s) 1178
PciI ACATGT 1 cut(s) 710
PciSI GCTCTTC 1 cut(s) 1311
PcsI WCGNNNNNNNCGW 1 cut(s) 922
PdmI GAANNNNTTC 1 cut(s) 1178
PfeI GAWTC 3 cut(s) 766, 1033, 1120
PflFI GACNNNGTC 1 cut(s) 1253
PflMI CCANNNNNTGG 2 cut(s) 665, 920
PkrI GCNGC 3 cut(s) 830, 907, 956
PleI GAGTC 1 cut(s) 713
PpsI GAGTC 1 cut(s) 713
PpuMI RGGWCCY 1 cut(s) 444
PscI ACATGT 1 cut(s) 710
Psp124BI GAGCTC 1 cut(s) 867
Psp5II RGGWCCY 1 cut(s) 444
PspN4I GGNNCC 5 cut(s) 41, 398, 445, 446, 832
PspPI GGNCC 4 cut(s) 199, 236, 444, 601
PspPPI RGGWCCY 1 cut(s) 444
PspXI VCTCGAGB 1 cut(s) 203
PsuI RGATCY 1 cut(s) 910
PsyI GACNNNGTC 1 cut(s) 1253
RsaI GTAC 3 cut(s) 476, 1193, 1274
RsaNI GTAC 3 cut(s) 475, 1192, 1273
RseI CAYNNNNRTG 1 cut(s) 1160
SacI GAGCTC 1 cut(s) 867
SacII CCGCGG 2 cut(s) 829, 955
SapI GCTCTTC 1 cut(s) 1311
SaqAI TTAA 1 cut(s) 227
SatI GCNGC 3 cut(s) 829, 906, 955
Sau3AI GATC 7 cut(s) 87, 91, 431, 462, 745, 910, 990
Sau96I GGNCC 4 cut(s) 199, 236, 444, 601
SchI GAGTC 1 cut(s) 714
ScrFI CCNGG 4 cut(s) 210, 728, 729, 1078
SduI GDGCHC 3 cut(s) 850, 867, 968
SfaNI GCATC 3 cut(s) 331, 947, 1270
SfcI CTRYAG 1 cut(s) 96
Sfr274I CTCGAG 1 cut(s) 203
Sfr303I CCGCGG 2 cut(s) 829, 955
SfuI TTCGAA 1 cut(s) 751
SgrBI CCGCGG 2 cut(s) 829, 955
SinI GGWCC 4 cut(s) 199, 236, 444, 601
SlaI CTCGAG 1 cut(s) 203
SmaI CCCGGG 1 cut(s) 729
SmiMI CAYNNNNRTG 1 cut(s) 1160
SmlI CTYRAG 4 cut(s) 131, 203, 226, 644
SmoI CTYRAG 4 cut(s) 131, 203, 226, 644
Sse9I AATT 6 cut(s) 45, 298, 332, 376, 572, 1108
SseBI AGGCCT 1 cut(s) 1178
SsiI CCGC 8 cut(s) 721, 784, 826, 828, 906, 952, 954, 1138
SspMI CTAG 3 cut(s) 183, 416, 758
SstI GAGCTC 1 cut(s) 867
StuI AGGCCT 1 cut(s) 1178
StyD4I CCNGG 4 cut(s) 208, 726, 727, 1076
StyI CCWWGG 2 cut(s) 182, 919
TaaI ACNGT 2 cut(s) 535, 1159
TaqI TCGA 8 cut(s) 90, 204, 540, 689, 751, 925, 1112, 1118
TasI AATT 6 cut(s) 45, 298, 332, 376, 572, 1108
TauI GCSGC 3 cut(s) 831, 908, 957
TfiI GAWTC 3 cut(s) 766, 1033, 1120
Tru1I TTAA 1 cut(s) 227
Tru9I TTAA 1 cut(s) 227
TscAI CASTG 2 cut(s) 195, 540
TseFI GTSAC 3 cut(s) 74, 706, 1061
Tsp45I GTSAC 3 cut(s) 74, 706, 1061
TspDTI ATGAA 5 cut(s) 171, 627, 787, 1090, 1259
TspMI CCCGGG 1 cut(s) 727
TspRI CASTG 2 cut(s) 195, 540
Tth111I GACNNNGTC 1 cut(s) 1253
Van91I CCANNNNNTGG 2 cut(s) 665, 920
Vha464I CTTAAG 1 cut(s) 226
VpaK11BI GGWCC 4 cut(s) 199, 236, 444, 601
XapI RAATTY 1 cut(s) 1108
XceI RCATGY 1 cut(s) 714
XcmI CCANNNNNNNNNTGG 1 cut(s) 287
XhoI CTCGAG 1 cut(s) 203
XmaI CCCGGG 1 cut(s) 727
XmaJI CCTAGG 1 cut(s) 182
XmnI GAANNNNTTC 1 cut(s) 1178
XspI CTAG 3 cut(s) 183, 416, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.