MD14G1103900.v1.1

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0017484
Physical Location & Seq
Reverse (-)
16040303 .. 16042726
2424 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1103900.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGAGAGAAATCTTGCATGTTCAAGCTGGACAATGTGGGAACCAAATTGGAGGCAAATTTTGGGAGGTGATGTGTGACGAGCATGGGATCGATCCCACGGGAAAATACAACGGGAACTCTCACCTTCAGCTTGAGAGGGTGAATGTTTACTATAATGAAGCAAATGGAGGGAGATATGTGCCTAGGGCAGTGCTGATGGACCTCGAGCCGGGGACGATGGACAGTTTAAGAACAGGTCCTTATGGGCAGATTTTCAGGCCGGACAACTTTGTGTTTGGTCAGAATGGAGCTGGAAACAATTGGGCTAAAGGGCATTACACAGAAGGAGCTGAGTTGATCGATGCTGTTCTTGATGTGGTTAGAAAAGAGGCCGAGAATTGTGATTGTTTACAAGGGTTCCAGATTTGCCATTCACTGGGAGGTGGGACAGGGTCAGGAATGGGAACCCTACTGATATCAAAGATCAGGGAAGAGTATCCAGATAGAATGATGATGACTTTCTCAGTCTTCCCCTCACCAAAAGTCTCAGACACTGTGGTTGAACCCTACAATGCCACTCTCTCTGTTCACCAGTTAGTTGAAAATGCTGATGAGGTCATGGTCCTTGACAATGAAGCCCTCTATGATATCTGTTTCAGAACTCTGAAGCTCACAAATCCAAGCTTCGGTGATTTGAATCATTTGATTTCCACAACCATGAGTGGAGTCACATGCTGCCTTCGCTTCCCGGGCCAGCTCAACTCCGACCTTCGAAAACTAGCTGTGAATCTCATCCCTTTCCCCCGCCTCCACTTTTTCATGGTTGGTTTCGCACCCTTGACCTCTCGCGGCTCCCAATTCTACAGAGCCCTCACAATCCCTGAGCTCACTCAACAAATGTGGGACGCTAAGAATATGATGTGCGCCGCAGATCCACGCCACGGTCGATACCTGACAGCATCGGCCGTGTTCCGTGGAAAAATGAGCACAAAAGAAGTGGACGAGCAAATGATCAACATACAAAACAAGAACTCCTCATTCTTTGTGGAGTGGATTCCAAACAATGTGAAATCAAGTGTTTGTGACATTCCACCAACCGGGCTCGCCATGTCCTCCACATTCATGGGGAATTCAACGTCAATTCAAGAGATGTTTAGGCGGGTTTCGGAGCAGTTCACGGTTATGTTTAGGAGGAAGGCCTTCTTGCACTGGTACACAGGTGAGGGCATGGATGAGATGGAGTTCACTGAGGCTGAGAGCAACATGAATGACTTGGTTTCTGAGTATCAGCAGTACCAAGATGCTGTGGCACAAGATGAGGGAGAGTATGAGGAGGAAGAGCTGGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

444

Amino Acids

49.95

Weight (kDa)

4.85

Isoelectric Point (pI)

32.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 3 - 211 2.2e-65 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 261 - 382 3.3e-39 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017484)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g02340
malus_domestica MD06G1082200.v1.1 MD14G1103900.v1.1
prunus_persica Prupe.5G099500_v2.0.a1
pyrus_communis pycom14g09990
rosa_chinensis RchiOBHm_Chr7g0197791
rosa_laevigata RLG00000003907
rosa_multiflora Rmu_sc0016786.1_g000003
rosa_roxburghii Rroxscaffold_3G00258200
rosa_rugosa Rorug07G0043700
rosa_samantha Rh7CG176800
rosa_wichuraiana Rw7G014590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 415
AccII CGCG 1 cut(s) 828
AciI CCGC 4 cut(s) 784, 828, 906, 1138
AclWI GGATC 3 cut(s) 86, 95, 905
AcoI YGGCCR 1 cut(s) 942
AcsI RAATTY 2 cut(s) 56, 1108
AcuI CTGAAG 2 cut(s) 110, 665
AfaI GTAC 2 cut(s) 1193, 1274
AfiI CCNNNNNNNGG 5 cut(s) 208, 415, 665, 920, 1076
AgsI TTSAA 6 cut(s) 23, 542, 581, 676, 1113, 1124
Alw21I GWGCWC 2 cut(s) 867, 968
Alw26I GTCTC 1 cut(s) 529
AlwI GGATC 3 cut(s) 86, 95, 905
AlwNI CAGNNNCTG 1 cut(s) 533
Ama87I CYCGRG 2 cut(s) 203, 727
AoxI GGCC 5 cut(s) 257, 369, 730, 942, 1176
ApeKI GCWGC 1 cut(s) 714
ApoI RAATTY 2 cut(s) 56, 1108
Asp700I GAANNNNTTC 1 cut(s) 1178
AspA2I CCTAGG 1 cut(s) 182
AspLEI GCGC 1 cut(s) 905
AspS9I GGNCC 4 cut(s) 199, 236, 601, 730
AsuC2I CCSGG 4 cut(s) 210, 728, 729, 1078
AsuHPI GGTGA 7 cut(s) 79, 113, 151, 507, 560, 680, 1211
AsuII TTCGAA 1 cut(s) 751
AvaI CYCGRG 2 cut(s) 203, 727
AvaII GGWCC 3 cut(s) 199, 236, 601
AvrII CCTAGG 1 cut(s) 182
BaeI ACNNNNGTAYC 2 cut(s) 1183, 1216
BanII GRGCYC 3 cut(s) 850, 867, 1083
BbsI GAAGAC 1 cut(s) 499
Bbv12I GWGCWC 2 cut(s) 867, 968
BbvI GCAGC 1 cut(s) 701
BccI CCATC 3 cut(s) 190, 211, 1210
BceAI ACGGC 1 cut(s) 929
BciVI GTATCC 1 cut(s) 486
BclI TGATCA 1 cut(s) 990
BcnI CCSGG 4 cut(s) 210, 728, 729, 1078
BcoDI GTCTC 1 cut(s) 529
BfaI CTAG 2 cut(s) 183, 758
BfmI CTRYAG 1 cut(s) 841
BfuI GTATCC 1 cut(s) 486
BisI GCNGC 3 cut(s) 715, 829, 906
BlnI CCTAGG 1 cut(s) 182
BlsI GCNGC 3 cut(s) 716, 830, 907
Bme1390I CCNGG 4 cut(s) 210, 728, 729, 1078
Bme18I GGWCC 3 cut(s) 199, 236, 601
BmeT110I CYCGRG 2 cut(s) 203, 727
BmgT120I GGNCC 4 cut(s) 199, 236, 601, 730
BmiI GGNNCC 4 cut(s) 41, 398, 445, 832
BmrFI CCNGG 4 cut(s) 210, 728, 729, 1078
BmrI ACTGGG 1 cut(s) 425
BmsI GCATC 3 cut(s) 331, 947, 1270
BmuI ACTGGG 1 cut(s) 425
BpiI GAAGAC 1 cut(s) 499
Bpu10I CCTNAGC 1 cut(s) 861
Bpu14I TTCGAA 1 cut(s) 751
BpuEI CTTGAG 1 cut(s) 152
BpuMI CCSGG 4 cut(s) 210, 728, 729, 1078
Bsa29I ATCGAT 2 cut(s) 90, 339
BsaBI GATNNNNATC 1 cut(s) 675
BsaJI CCNNGG 6 cut(s) 96, 182, 209, 727, 919, 952
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 995, 1025
Bsc4I CCNNNNNNNGG 5 cut(s) 208, 415, 665, 920, 1076
Bse1I ACTGG 3 cut(s) 420, 571, 1193
Bse8I GATNNNNATC 1 cut(s) 675
BseCI ATCGAT 2 cut(s) 90, 339
BseDI CCNNGG 6 cut(s) 96, 182, 209, 727, 919, 952
BseGI GGATG 2 cut(s) 771, 1216
BseJI GATNNNNATC 1 cut(s) 675
BseLI CCNNNNNNNGG 5 cut(s) 208, 415, 665, 920, 1076
BseMII CTCAG 7 cut(s) 321, 516, 540, 852, 1218, 1224, 1251
BseNI ACTGG 3 cut(s) 420, 571, 1193
BseRI GAGGAG 2 cut(s) 1003, 1325
BseX3I CGGCCG 1 cut(s) 942
BseXI GCAGC 1 cut(s) 701
Bsh1236I CGCG 1 cut(s) 828
Bsh1285I CGRYCG 2 cut(s) 925, 945
BshFI GGCC 5 cut(s) 259, 371, 732, 944, 1178
BshVI ATCGAT 2 cut(s) 90, 339
BsiEI CGRYCG 2 cut(s) 925, 945
BsiHKAI GWGCWC 2 cut(s) 867, 968
BsiHKCI CYCGRG 2 cut(s) 203, 727
BsiSI CCGG 4 cut(s) 209, 260, 728, 1077
BslFI GGGAC 3 cut(s) 226, 439, 896
BslI CCNNNNNNNGG 5 cut(s) 208, 415, 665, 920, 1076
BsmAI GTCTC 1 cut(s) 529
BsmFI GGGAC 3 cut(s) 226, 439, 896
BsnI GGCC 5 cut(s) 259, 371, 732, 944, 1178
BsoBI CYCGRG 2 cut(s) 203, 727
Bsp119I TTCGAA 1 cut(s) 751
Bsp1286I GDGCHC 4 cut(s) 850, 867, 968, 1083
Bsp143I GATC 6 cut(s) 87, 91, 336, 462, 910, 990
BspACI CCGC 4 cut(s) 784, 828, 906, 1138
BspANI GGCC 5 cut(s) 259, 371, 732, 944, 1178
BspCNI CTCAG 7 cut(s) 322, 515, 539, 853, 1219, 1225, 1252
BspDI ATCGAT 2 cut(s) 90, 339
BspFNI CGCG 1 cut(s) 828
BspLI GGNNCC 4 cut(s) 41, 398, 445, 832
BspPI GGATC 3 cut(s) 86, 95, 905
BspQI GCTCTTC 1 cut(s) 1311
BspT104I TTCGAA 1 cut(s) 751
BsrI ACTGG 3 cut(s) 420, 571, 1193
BssECI CCNNGG 6 cut(s) 96, 182, 209, 727, 919, 952
BssMI GATC 6 cut(s) 87, 91, 336, 462, 910, 990
BssT1I CCWWGG 1 cut(s) 182
Bst4CI ACNGT 4 cut(s) 224, 535, 923, 1159
Bst6I CTCTTC 2 cut(s) 465, 1311
BstBI TTCGAA 1 cut(s) 751
BstC8I GCNNGC 2 cut(s) 734, 1083
BstDEI CTNAG 8 cut(s) 330, 502, 526, 861, 888, 1227, 1233, 1260
BstDSI CCRYGG 3 cut(s) 96, 919, 952
BstF5I GGATG 2 cut(s) 771, 1216
BstFNI CGCG 1 cut(s) 828
BstHHI GCGC 1 cut(s) 905
BstKTI GATC 6 cut(s) 90, 94, 339, 465, 913, 993
BstMAI GTCTC 1 cut(s) 529
BstMBI GATC 6 cut(s) 87, 91, 336, 462, 910, 990
BstMCI CGRYCG 2 cut(s) 925, 945
BstMWI GCNNNNNNNGC 2 cut(s) 720, 729
BstNSI RCATGY 2 cut(s) 20, 714
BstSCI CCNGG 4 cut(s) 208, 726, 727, 1076
BstSFI CTRYAG 1 cut(s) 841
BstUI CGCG 1 cut(s) 828
BstV1I GCAGC 1 cut(s) 701
BstV2I GAAGAC 1 cut(s) 499
BstX2I RGATCY 1 cut(s) 910
BstXI CCANNNNNNTGG 1 cut(s) 1102
BstYI RGATCY 1 cut(s) 910
BstZI CGGCCG 1 cut(s) 942
Bsu15I ATCGAT 2 cut(s) 90, 339
BsuI GTATCC 1 cut(s) 486
BsuRI GGCC 5 cut(s) 259, 371, 732, 944, 1178
BsuTUI ATCGAT 2 cut(s) 90, 339
BtgI CCRYGG 3 cut(s) 96, 919, 952
BtsCI GGATG 2 cut(s) 771, 1216
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 5 cut(s) 195, 413, 531, 1186, 1224
Cac8I GCNNGC 2 cut(s) 734, 1083
CaiI CAGNNNCTG 1 cut(s) 533
CfoI GCGC 1 cut(s) 905
Cfr13I GGNCC 4 cut(s) 199, 236, 601, 730
Cfr9I CCCGGG 1 cut(s) 727
ClaI ATCGAT 2 cut(s) 90, 339
CseI GACGC 1 cut(s) 893
Csp6I GTAC 2 cut(s) 1192, 1273
CspCI CAANNNNNGTGG 4 cut(s) 860, 895, 957, 992
CviQI GTAC 2 cut(s) 1192, 1273
DdeI CTNAG 8 cut(s) 330, 502, 526, 861, 888, 1227, 1233, 1260
DpnI GATC 6 cut(s) 89, 93, 338, 464, 912, 992
DpnII GATC 6 cut(s) 87, 91, 336, 462, 910, 990
EaeI YGGCCR 1 cut(s) 942
EagI CGGCCG 1 cut(s) 942
Eam1104I CTCTTC 2 cut(s) 465, 1311
EarI CTCTTC 2 cut(s) 465, 1311
Ecl136II GAGCTC 1 cut(s) 865
EclXI CGGCCG 1 cut(s) 942
Eco130I CCWWGG 1 cut(s) 182
Eco147I AGGCCT 1 cut(s) 1178
Eco24I GRGCYC 3 cut(s) 850, 867, 1083
Eco32I GATATC 2 cut(s) 456, 628
Eco47I GGWCC 3 cut(s) 199, 236, 601
Eco52I CGGCCG 1 cut(s) 942
Eco53kI GAGCTC 1 cut(s) 865
Eco57I CTGAAG 2 cut(s) 110, 665
Eco88I CYCGRG 2 cut(s) 203, 727
EcoICRI GAGCTC 1 cut(s) 865
EcoO109I RGGNCCY 1 cut(s) 236
EcoRI GAATTC 1 cut(s) 1108
EcoRV GATATC 2 cut(s) 456, 628
EcoT14I CCWWGG 1 cut(s) 182
EcoT38I GRGCYC 3 cut(s) 850, 867, 1083
ErhI CCWWGG 1 cut(s) 182
FalI AAGNNNNNCTT 2 cut(s) 1166, 1198
FaqI GGGAC 3 cut(s) 226, 439, 896
FauI CCCGC 2 cut(s) 791, 1131
FbaI TGATCA 1 cut(s) 990
Fnu4HI GCNGC 3 cut(s) 715, 829, 906
FokI GGATG 2 cut(s) 758, 1223
FriOI GRGCYC 3 cut(s) 850, 867, 1083
Fsp4HI GCNGC 3 cut(s) 715, 829, 906
FspBI CTAG 2 cut(s) 183, 758
GlaI GCGC 1 cut(s) 904
GluI GCNGC 3 cut(s) 715, 829, 906
HaeIII GGCC 5 cut(s) 259, 371, 732, 944, 1178
HapII CCGG 4 cut(s) 209, 260, 728, 1077
HgaI GACGC 1 cut(s) 893
HhaI GCGC 1 cut(s) 905
Hin6I GCGC 1 cut(s) 903
HinP1I GCGC 1 cut(s) 903
HindIII AAGCTT 1 cut(s) 661
HinfI GANTC 4 cut(s) 676, 705, 766, 1033
HpaII CCGG 4 cut(s) 209, 260, 728, 1077
HphI GGTGA 7 cut(s) 79, 113, 151, 507, 560, 680, 1211
Hpy166II GTNNAC 7 cut(s) 148, 389, 568, 979, 1155, 1194, 1224
Hpy188I TCNGA 7 cut(s) 282, 529, 638, 645, 745, 1147, 1261
Hpy188III TCNNGA 5 cut(s) 350, 400, 435, 479, 1124
Hpy8I GTNNAC 7 cut(s) 148, 389, 568, 979, 1155, 1194, 1224
HpyAV CCTTC 6 cut(s) 134, 317, 728, 758, 1168, 1189
HpyCH4III ACNGT 4 cut(s) 224, 535, 923, 1159
HpyCH4IV ACGT 1 cut(s) 1115
HpyCH4V TGCA 2 cut(s) 16, 1186
HpyF10VI GCNNNNNNNGC 2 cut(s) 720, 729
HpyF3I CTNAG 8 cut(s) 330, 502, 526, 861, 888, 1227, 1233, 1260
HpySE526I ACGT 1 cut(s) 1115
HspAI GCGC 1 cut(s) 903
Ksp22I TGATCA 1 cut(s) 990
Kzo9I GATC 6 cut(s) 87, 91, 336, 462, 910, 990
LguI GCTCTTC 1 cut(s) 1311
LmnI GCTCC 4 cut(s) 287, 326, 836, 1147
Lsp1109I GCAGC 1 cut(s) 701
LweI GCATC 3 cut(s) 331, 947, 1270
MaeI CTAG 2 cut(s) 183, 758
MaeII ACGT 1 cut(s) 1115
MaeIII GTNAC 3 cut(s) 74, 706, 1061
MalI GATC 6 cut(s) 89, 93, 338, 464, 912, 992
MboI GATC 6 cut(s) 87, 91, 336, 462, 910, 990
MboII GAAGA 3 cut(s) 482, 499, 1328
MfeI CAATTG 1 cut(s) 298
MflI RGATCY 1 cut(s) 910
MhlI GDGCHC 4 cut(s) 850, 867, 968, 1083
MluCI AATT 7 cut(s) 45, 56, 298, 376, 836, 1108, 1119
MlyI GAGTC 1 cut(s) 714
MmeI TCCRAC 1 cut(s) 768
MroXI GAANNNNTTC 1 cut(s) 1178
MseI TTAA 1 cut(s) 227
MslI CAYNNNNRTG 3 cut(s) 695, 1100, 1160
MspI CCGG 4 cut(s) 209, 260, 728, 1077
MspR9I CCNGG 4 cut(s) 210, 728, 729, 1078
MunI CAATTG 1 cut(s) 298
MvnI CGCG 1 cut(s) 828
MwoI GCNNNNNNNGC 2 cut(s) 720, 729
NciI CCSGG 4 cut(s) 210, 728, 729, 1078
NdeII GATC 6 cut(s) 87, 91, 336, 462, 910, 990
NlaIV GGNNCC 4 cut(s) 41, 398, 445, 832
NmeAIII GCCGAG 1 cut(s) 397
NmuCI GTSAC 3 cut(s) 74, 706, 1061
NspI RCATGY 2 cut(s) 20, 714
NspV TTCGAA 1 cut(s) 751
PaeR7I CTCGAG 1 cut(s) 203
PceI AGGCCT 1 cut(s) 1178
PciSI GCTCTTC 1 cut(s) 1311
PcsI WCGNNNNNNNCGW 1 cut(s) 922
PdmI GAANNNNTTC 1 cut(s) 1178
PfeI GAWTC 3 cut(s) 676, 766, 1033
PflFI GACNNNGTC 1 cut(s) 430
PflMI CCANNNNNTGG 1 cut(s) 415
PkrI GCNGC 3 cut(s) 716, 830, 907
PleI GAGTC 1 cut(s) 713
PpsI GAGTC 1 cut(s) 713
PpuMI RGGWCCY 1 cut(s) 236
Psp124BI GAGCTC 1 cut(s) 867
Psp5II RGGWCCY 1 cut(s) 236
PspN4I GGNNCC 4 cut(s) 41, 398, 445, 832
PspPI GGNCC 4 cut(s) 199, 236, 601, 730
PspPPI RGGWCCY 1 cut(s) 236
PspXI VCTCGAGB 1 cut(s) 203
PstNI CAGNNNCTG 1 cut(s) 533
PsuI RGATCY 1 cut(s) 910
PsyI GACNNNGTC 1 cut(s) 430
RsaI GTAC 2 cut(s) 1193, 1274
RsaNI GTAC 2 cut(s) 1192, 1273
RseI CAYNNNNRTG 3 cut(s) 695, 1100, 1160
SacI GAGCTC 1 cut(s) 867
SapI GCTCTTC 1 cut(s) 1311
SaqAI TTAA 1 cut(s) 227
SatI GCNGC 3 cut(s) 715, 829, 906
Sau3AI GATC 6 cut(s) 87, 91, 336, 462, 910, 990
Sau96I GGNCC 4 cut(s) 199, 236, 601, 730
SchI GAGTC 1 cut(s) 714
ScrFI CCNGG 4 cut(s) 210, 728, 729, 1078
SduI GDGCHC 4 cut(s) 850, 867, 968, 1083
SfaNI GCATC 3 cut(s) 331, 947, 1270
SfcI CTRYAG 1 cut(s) 841
Sfr274I CTCGAG 1 cut(s) 203
SfuI TTCGAA 1 cut(s) 751
SinI GGWCC 3 cut(s) 199, 236, 601
SlaI CTCGAG 1 cut(s) 203
SmaI CCCGGG 1 cut(s) 729
SmiMI CAYNNNNRTG 3 cut(s) 695, 1100, 1160
SmlI CTYRAG 2 cut(s) 131, 203
SmoI CTYRAG 2 cut(s) 131, 203
Sse9I AATT 7 cut(s) 45, 56, 298, 376, 836, 1108, 1119
SseBI AGGCCT 1 cut(s) 1178
SsiI CCGC 4 cut(s) 784, 828, 906, 1138
SspMI CTAG 2 cut(s) 183, 758
SstI GAGCTC 1 cut(s) 867
StuI AGGCCT 1 cut(s) 1178
StyD4I CCNGG 4 cut(s) 208, 726, 727, 1076
StyI CCWWGG 1 cut(s) 182
TaaI ACNGT 4 cut(s) 224, 535, 923, 1159
TaiI ACGT 1 cut(s) 1118
TaqI TCGA 5 cut(s) 90, 204, 339, 751, 925
TasI AATT 7 cut(s) 45, 56, 298, 376, 836, 1108, 1119
TauI GCSGC 2 cut(s) 831, 908
TfiI GAWTC 3 cut(s) 676, 766, 1033
Tru1I TTAA 1 cut(s) 227
Tru9I TTAA 1 cut(s) 227
TscAI CASTG 5 cut(s) 195, 420, 538, 1193, 1231
TseFI GTSAC 3 cut(s) 74, 706, 1061
TseI GCWGC 1 cut(s) 714
Tsp45I GTSAC 3 cut(s) 74, 706, 1061
TspDTI ATGAA 5 cut(s) 171, 627, 787, 1090, 1259
TspGWI ACGGA 1 cut(s) 941
TspMI CCCGGG 1 cut(s) 727
TspRI CASTG 5 cut(s) 195, 420, 538, 1193, 1231
Tth111I GACNNNGTC 1 cut(s) 430
Van91I CCANNNNNTGG 1 cut(s) 415
VpaK11BI GGWCC 3 cut(s) 199, 236, 601
XapI RAATTY 2 cut(s) 56, 1108
XceI RCATGY 2 cut(s) 20, 714
XhoI CTCGAG 1 cut(s) 203
XmaI CCCGGG 1 cut(s) 727
XmaJI CCTAGG 1 cut(s) 182
XmnI GAANNNNTTC 1 cut(s) 1178
XspI CTAG 2 cut(s) 183, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.