Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g02340 |
| malus_domestica | MD06G1082200.v1.1 MD14G1103900.v1.1 |
| prunus_persica | Prupe.5G099500_v2.0.a1 |
| pyrus_communis | pycom14g09990 |
| rosa_chinensis | RchiOBHm_Chr7g0197791 |
| rosa_laevigata | RLG00000003907 |
| rosa_multiflora | Rmu_sc0016786.1_g000003 |
| rosa_roxburghii | Rroxscaffold_3G00258200 |
| rosa_rugosa | Rorug07G0043700 |
| rosa_samantha | Rh7CG176800 |
| rosa_wichuraiana | Rw7G014590 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 3 cut(s) | 415, 665, 1076 |
| AccII | CGCG | 2 cut(s) | 812, 954 |
| AciI | CCGC | 6 cut(s) | 784, 906, 925, 942, 952, 954 |
| AclWI | GGATC | 4 cut(s) | 86, 207, 1027, 1040 |
| AcoI | YGGCCR | 3 cut(s) | 277, 922, 1083 |
| AcsI | RAATTY | 3 cut(s) | 56, 115, 249 |
| AcuI | CTGAAG | 2 cut(s) | 110, 619 |
| AcyI | GRCGYC | 1 cut(s) | 885 |
| AfaI | GTAC | 4 cut(s) | 149, 1193, 1265, 1274 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 415, 665, 727, 920, 1076, 1144 |
| AflII | CTTAAG | 1 cut(s) | 226 |
| AgsI | TTSAA | 2 cut(s) | 23, 542 |
| AjnI | CCWGG | 3 cut(s) | 208, 1122, 1275 |
| AluBI | AGCT | 8 cut(s) | 26, 130, 225, 329, 649, 663, 757, 865 |
| AluI | AGCT | 8 cut(s) | 26, 130, 225, 329, 649, 663, 757, 865 |
| Alw21I | GWGCWC | 2 cut(s) | 867, 968 |
| AlwI | GGATC | 4 cut(s) | 86, 207, 1027, 1040 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 434 |
| Ama87I | CYCGRG | 2 cut(s) | 727, 825 |
| AoxI | GGCC | 7 cut(s) | 257, 277, 730, 846, 922, 1083, 1176 |
| ApaI | GGGCCC | 1 cut(s) | 850 |
| ApeKI | GCWGC | 1 cut(s) | 714 |
| ApoI | RAATTY | 3 cut(s) | 56, 115, 249 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1178 |
| AspLEI | GCGC | 3 cut(s) | 814, 905, 1291 |
| AspS9I | GGNCC | 6 cut(s) | 213, 236, 601, 730, 846, 847 |
| AsuC2I | CCSGG | 2 cut(s) | 728, 729 |
| AsuHPI | GGTGA | 3 cut(s) | 151, 507, 680 |
| AsuNHI | GCTAGC | 1 cut(s) | 757 |
| AvaI | CYCGRG | 2 cut(s) | 727, 825 |
| AvaII | GGWCC | 3 cut(s) | 213, 236, 601 |
| BaeGI | GKGCMC | 1 cut(s) | 850 |
| BalI | TGGCCA | 2 cut(s) | 279, 1085 |
| BamHI | GGATCC | 1 cut(s) | 1032 |
| BanII | GRGCYC | 3 cut(s) | 833, 850, 867 |
| BbsI | GAAGAC | 2 cut(s) | 499, 940 |
| Bbv12I | GWGCWC | 2 cut(s) | 867, 968 |
| BbvI | GCAGC | 1 cut(s) | 701 |
| BccI | CCATC | 3 cut(s) | 190, 433, 1210 |
| BceAI | ACGGC | 4 cut(s) | 746, 929, 937, 1122 |
| BciT130I | CCWGG | 3 cut(s) | 210, 1124, 1277 |
| BciVI | GTATCC | 1 cut(s) | 486 |
| BclI | TGATCA | 1 cut(s) | 990 |
| BcnI | CCSGG | 2 cut(s) | 728, 729 |
| BfaI | CTAG | 2 cut(s) | 183, 758 |
| BfmI | CTRYAG | 1 cut(s) | 841 |
| BfrI | CTTAAG | 1 cut(s) | 226 |
| BfuI | GTATCC | 1 cut(s) | 486 |
| BisI | GCNGC | 4 cut(s) | 715, 906, 925, 955 |
| BlsI | GCNGC | 4 cut(s) | 716, 907, 926, 956 |
| Bme1390I | CCNGG | 5 cut(s) | 210, 728, 729, 1124, 1277 |
| Bme18I | GGWCC | 3 cut(s) | 213, 236, 601 |
| BmeT110I | CYCGRG | 2 cut(s) | 727, 825 |
| BmgT120I | GGNCC | 6 cut(s) | 213, 236, 601, 730, 846, 847 |
| BmiI | GGNNCC | 5 cut(s) | 214, 398, 832, 848, 1034 |
| BmrFI | CCNGG | 5 cut(s) | 210, 728, 729, 1124, 1277 |
| BmrI | ACTGGG | 3 cut(s) | 310, 425, 1086 |
| BmsI | GCATC | 2 cut(s) | 947, 1270 |
| BmtI | GCTAGC | 1 cut(s) | 761 |
| BmuI | ACTGGG | 3 cut(s) | 310, 425, 1086 |
| BpiI | GAAGAC | 2 cut(s) | 499, 940 |
| BpuEI | CTTGAG | 3 cut(s) | 152, 224, 629 |
| BpuMI | CCSGG | 2 cut(s) | 728, 729 |
| BsaHI | GRCGYC | 1 cut(s) | 885 |
| BsaJI | CCNNGG | 5 cut(s) | 209, 216, 727, 919, 952 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 56, 86, 995, 1025 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 415, 665, 727, 920, 1076, 1144 |
| Bse1I | ACTGG | 4 cut(s) | 103, 305, 420, 1081 |
| Bse3DI | GCAATG | 1 cut(s) | 38 |
| BseBI | CCWGG | 3 cut(s) | 210, 1124, 1277 |
| BseDI | CCNNGG | 5 cut(s) | 209, 216, 727, 919, 952 |
| BseGI | GGATG | 7 cut(s) | 444, 771, 938, 985, 1216, 1285, 1300 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 415, 665, 727, 920, 1076, 1144 |
| BseMI | GCAATG | 1 cut(s) | 38 |
| BseMII | CTCAG | 4 cut(s) | 321, 516, 1218, 1251 |
| BseNI | ACTGG | 4 cut(s) | 103, 305, 420, 1081 |
| BseRI | GAGGAG | 3 cut(s) | 485, 1003, 1328 |
| BseSI | GKGCMC | 1 cut(s) | 850 |
| BseX3I | CGGCCG | 1 cut(s) | 922 |
| BseXI | GCAGC | 1 cut(s) | 701 |
| BseYI | CCCAGC | 1 cut(s) | 26 |
| Bsh1236I | CGCG | 2 cut(s) | 812, 954 |
| Bsh1285I | CGRYCG | 1 cut(s) | 925 |
| BshFI | GGCC | 7 cut(s) | 259, 279, 732, 848, 924, 1085, 1178 |
| BsiEI | CGRYCG | 1 cut(s) | 925 |
| BsiHKAI | GWGCWC | 2 cut(s) | 867, 968 |
| BsiHKCI | CYCGRG | 2 cut(s) | 727, 825 |
| BsiSI | CCGG | 1 cut(s) | 728 |
| BslFI | GGGAC | 3 cut(s) | 226, 457, 896 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 415, 665, 727, 920, 1076, 1144 |
| BsmFI | GGGAC | 3 cut(s) | 226, 457, 896 |
| BsnI | GGCC | 7 cut(s) | 259, 279, 732, 848, 924, 1085, 1178 |
| BsoBI | CYCGRG | 2 cut(s) | 727, 825 |
| Bsp120I | GGGCCC | 1 cut(s) | 846 |
| Bsp1286I | GDGCHC | 4 cut(s) | 833, 850, 867, 968 |
| Bsp143I | GATC | 7 cut(s) | 91, 199, 462, 670, 684, 990, 1032 |
| Bsp19I | CCATGG | 1 cut(s) | 216 |
| BspACI | CCGC | 6 cut(s) | 784, 906, 925, 942, 952, 954 |
| BspANI | GGCC | 7 cut(s) | 259, 279, 732, 848, 924, 1085, 1178 |
| BspCNI | CTCAG | 4 cut(s) | 322, 515, 1219, 1252 |
| BspFNI | CGCG | 2 cut(s) | 812, 954 |
| BspLI | GGNNCC | 5 cut(s) | 214, 398, 832, 848, 1034 |
| BspOI | GCTAGC | 1 cut(s) | 761 |
| BspPI | GGATC | 4 cut(s) | 86, 207, 1027, 1040 |
| BspTI | CTTAAG | 1 cut(s) | 226 |
| BsrDI | GCAATG | 1 cut(s) | 38 |
| BsrI | ACTGG | 4 cut(s) | 103, 305, 420, 1081 |
| BssECI | CCNNGG | 5 cut(s) | 209, 216, 727, 919, 952 |
| BssMI | GATC | 7 cut(s) | 91, 199, 462, 670, 684, 990, 1032 |
| BssNI | GRCGYC | 1 cut(s) | 885 |
| BssT1I | CCWWGG | 1 cut(s) | 216 |
| Bst2UI | CCWGG | 3 cut(s) | 210, 1124, 1277 |
| Bst4CI | ACNGT | 2 cut(s) | 535, 1159 |
| Bst6I | CTCTTC | 1 cut(s) | 1314 |
| BstACI | GRCGYC | 1 cut(s) | 885 |
| BstAFI | CTTAAG | 1 cut(s) | 226 |
| BstC8I | GCNNGC | 1 cut(s) | 759 |
| BstDEI | CTNAG | 4 cut(s) | 330, 502, 1227, 1260 |
| BstDSI | CCRYGG | 3 cut(s) | 216, 919, 952 |
| BstF5I | GGATG | 7 cut(s) | 444, 771, 938, 985, 1216, 1285, 1300 |
| BstFNI | CGCG | 2 cut(s) | 812, 954 |
| BstHHI | GCGC | 3 cut(s) | 814, 905, 1291 |
| BstKTI | GATC | 7 cut(s) | 94, 202, 465, 673, 687, 993, 1035 |
| BstMBI | GATC | 7 cut(s) | 91, 199, 462, 670, 684, 990, 1032 |
| BstMCI | CGRYCG | 1 cut(s) | 925 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 871, 924, 963 |
| BstNI | CCWGG | 3 cut(s) | 210, 1124, 1277 |
| BstNSI | RCATGY | 1 cut(s) | 714 |
| BstSCI | CCNGG | 5 cut(s) | 208, 726, 727, 1122, 1275 |
| BstSFI | CTRYAG | 1 cut(s) | 841 |
| BstSLI | GKGCMC | 1 cut(s) | 850 |
| BstUI | CGCG | 2 cut(s) | 812, 954 |
| BstV1I | GCAGC | 1 cut(s) | 701 |
| BstV2I | GAAGAC | 2 cut(s) | 499, 940 |
| BstX2I | RGATCY | 2 cut(s) | 199, 1032 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1102 |
| BstYI | RGATCY | 2 cut(s) | 199, 1032 |
| BstZI | CGGCCG | 1 cut(s) | 922 |
| BsuI | GTATCC | 1 cut(s) | 486 |
| BsuRI | GGCC | 7 cut(s) | 259, 279, 732, 848, 924, 1085, 1178 |
| BtgI | CCRYGG | 3 cut(s) | 216, 919, 952 |
| BtsCI | GGATG | 7 cut(s) | 444, 771, 938, 985, 1216, 1285, 1300 |
| BtsIMutI | CAGTG | 4 cut(s) | 96, 413, 531, 1224 |
| Cac8I | GCNNGC | 1 cut(s) | 759 |
| CaiI | CAGNNNCTG | 1 cut(s) | 434 |
| CfoI | GCGC | 3 cut(s) | 814, 905, 1291 |
| Cfr13I | GGNCC | 6 cut(s) | 213, 236, 601, 730, 846, 847 |
| Cfr42I | CCGCGG | 1 cut(s) | 955 |
| Cfr9I | CCCGGG | 1 cut(s) | 727 |
| CseI | GACGC | 1 cut(s) | 893 |
| Csp6I | GTAC | 4 cut(s) | 148, 1192, 1264, 1273 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 957, 992 |
| CviQI | GTAC | 4 cut(s) | 148, 1192, 1264, 1273 |
| DdeI | CTNAG | 4 cut(s) | 330, 502, 1227, 1260 |
| DpnI | GATC | 7 cut(s) | 93, 201, 464, 672, 686, 992, 1034 |
| DpnII | GATC | 7 cut(s) | 91, 199, 462, 670, 684, 990, 1032 |
| EaeI | YGGCCR | 3 cut(s) | 277, 922, 1083 |
| EagI | CGGCCG | 1 cut(s) | 922 |
| Eam1104I | CTCTTC | 1 cut(s) | 1314 |
| EarI | CTCTTC | 1 cut(s) | 1314 |
| EciI | GGCGGA | 1 cut(s) | 931 |
| Ecl136II | GAGCTC | 1 cut(s) | 865 |
| EclXI | CGGCCG | 1 cut(s) | 922 |
| Eco130I | CCWWGG | 1 cut(s) | 216 |
| Eco147I | AGGCCT | 2 cut(s) | 259, 1178 |
| Eco24I | GRGCYC | 3 cut(s) | 833, 850, 867 |
| Eco32I | GATATC | 2 cut(s) | 456, 628 |
| Eco47I | GGWCC | 3 cut(s) | 213, 236, 601 |
| Eco52I | CGGCCG | 1 cut(s) | 922 |
| Eco53kI | GAGCTC | 1 cut(s) | 865 |
| Eco57I | CTGAAG | 2 cut(s) | 110, 619 |
| Eco88I | CYCGRG | 2 cut(s) | 727, 825 |
| EcoICRI | GAGCTC | 1 cut(s) | 865 |
| EcoO109I | RGGNCCY | 3 cut(s) | 236, 846, 847 |
| EcoRII | CCWGG | 3 cut(s) | 208, 1122, 1275 |
| EcoRV | GATATC | 2 cut(s) | 456, 628 |
| EcoT14I | CCWWGG | 1 cut(s) | 216 |
| EcoT38I | GRGCYC | 3 cut(s) | 833, 850, 867 |
| ErhI | CCWWGG | 1 cut(s) | 216 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1166, 1198 |
| FaqI | GGGAC | 3 cut(s) | 226, 457, 896 |
| FauI | CCCGC | 1 cut(s) | 791 |
| FbaI | TGATCA | 1 cut(s) | 990 |
| Fnu4HI | GCNGC | 4 cut(s) | 715, 906, 925, 955 |
| FokI | GGATG | 7 cut(s) | 451, 758, 925, 992, 1223, 1292, 1307 |
| FriOI | GRGCYC | 3 cut(s) | 833, 850, 867 |
| Fsp4HI | GCNGC | 4 cut(s) | 715, 906, 925, 955 |
| FspBI | CTAG | 2 cut(s) | 183, 758 |
| GlaI | GCGC | 3 cut(s) | 813, 904, 1290 |
| GluI | GCNGC | 4 cut(s) | 715, 906, 925, 955 |
| GsaI | CCCAGC | 1 cut(s) | 30 |
| HaeIII | GGCC | 7 cut(s) | 259, 279, 732, 848, 924, 1085, 1178 |
| HapII | CCGG | 1 cut(s) | 728 |
| HgaI | GACGC | 1 cut(s) | 893 |
| HhaI | GCGC | 3 cut(s) | 814, 905, 1291 |
| Hin1I | GRCGYC | 1 cut(s) | 885 |
| Hin6I | GCGC | 3 cut(s) | 812, 903, 1289 |
| HinP1I | GCGC | 3 cut(s) | 812, 903, 1289 |
| HindIII | AAGCTT | 1 cut(s) | 661 |
| HinfI | GANTC | 4 cut(s) | 341, 445, 705, 723 |
| HpaII | CCGG | 1 cut(s) | 728 |
| HphI | GGTGA | 3 cut(s) | 151, 507, 680 |
| Hpy166II | GTNNAC | 4 cut(s) | 148, 766, 1194, 1224 |
| Hpy188I | TCNGA | 8 cut(s) | 529, 638, 675, 722, 745, 752, 1147, 1261 |
| Hpy188III | TCNNGA | 5 cut(s) | 193, 203, 350, 400, 479 |
| Hpy8I | GTNNAC | 4 cut(s) | 148, 766, 1194, 1224 |
| HpyAV | CCTTC | 8 cut(s) | 134, 317, 522, 787, 1027, 1168, 1189, 1196 |
| HpyCH4III | ACNGT | 2 cut(s) | 535, 1159 |
| HpyCH4IV | ACGT | 1 cut(s) | 1044 |
| HpyCH4V | TGCA | 4 cut(s) | 16, 391, 587, 1186 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 871, 924, 963 |
| HpyF3I | CTNAG | 4 cut(s) | 330, 502, 1227, 1260 |
| HpySE526I | ACGT | 1 cut(s) | 1044 |
| Hsp92I | GRCGYC | 1 cut(s) | 885 |
| HspAI | GCGC | 3 cut(s) | 812, 903, 1289 |
| Ksp22I | TGATCA | 1 cut(s) | 990 |
| KspI | CCGCGG | 1 cut(s) | 955 |
| Kzo9I | GATC | 7 cut(s) | 91, 199, 462, 670, 684, 990, 1032 |
| LmnI | GCTCC | 4 cut(s) | 287, 326, 836, 1147 |
| Lsp1109I | GCAGC | 1 cut(s) | 701 |
| LweI | GCATC | 2 cut(s) | 947, 1270 |
| MaeI | CTAG | 2 cut(s) | 183, 758 |
| MaeII | ACGT | 1 cut(s) | 1044 |
| MaeIII | GTNAC | 2 cut(s) | 706, 1061 |
| MalI | GATC | 7 cut(s) | 93, 201, 464, 672, 686, 992, 1034 |
| MboI | GATC | 7 cut(s) | 91, 199, 462, 670, 684, 990, 1032 |
| MboII | GAAGA | 4 cut(s) | 499, 787, 940, 1331 |
| MfeI | CAATTG | 1 cut(s) | 572 |
| MflI | RGATCY | 2 cut(s) | 199, 1032 |
| MhlI | GDGCHC | 4 cut(s) | 833, 850, 867, 968 |
| MlsI | TGGCCA | 2 cut(s) | 279, 1085 |
| MluCI | AATT | 8 cut(s) | 45, 56, 115, 249, 376, 572, 1151, 1330 |
| MluNI | TGGCCA | 2 cut(s) | 279, 1085 |
| MlyI | GAGTC | 2 cut(s) | 439, 714 |
| MmeI | TCCRAC | 1 cut(s) | 768 |
| Mox20I | TGGCCA | 2 cut(s) | 279, 1085 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1178 |
| MscI | TGGCCA | 2 cut(s) | 279, 1085 |
| MseI | TTAA | 2 cut(s) | 227, 818 |
| MslI | CAYNNNNRTG | 3 cut(s) | 695, 1100, 1160 |
| Msp20I | TGGCCA | 2 cut(s) | 279, 1085 |
| MspA1I | CMGCKG | 1 cut(s) | 954 |
| MspCI | CTTAAG | 1 cut(s) | 226 |
| MspI | CCGG | 1 cut(s) | 728 |
| MspR9I | CCNGG | 5 cut(s) | 210, 728, 729, 1124, 1277 |
| MunI | CAATTG | 1 cut(s) | 572 |
| MvaI | CCWGG | 3 cut(s) | 210, 1124, 1277 |
| MvnI | CGCG | 2 cut(s) | 812, 954 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 871, 924, 963 |
| NciI | CCSGG | 2 cut(s) | 728, 729 |
| NcoI | CCATGG | 1 cut(s) | 216 |
| NdeII | GATC | 7 cut(s) | 91, 199, 462, 670, 684, 990, 1032 |
| NheI | GCTAGC | 1 cut(s) | 757 |
| NlaIV | GGNNCC | 5 cut(s) | 214, 398, 832, 848, 1034 |
| NmuCI | GTSAC | 2 cut(s) | 706, 1061 |
| NspI | RCATGY | 1 cut(s) | 714 |
| PceI | AGGCCT | 2 cut(s) | 259, 1178 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1178 |
| PfeI | GAWTC | 2 cut(s) | 341, 723 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 415, 665, 1076 |
| PfoI | TCCNGGA | 1 cut(s) | 1122 |
| PkrI | GCNGC | 4 cut(s) | 716, 907, 926, 956 |
| PleI | GAGTC | 2 cut(s) | 439, 713 |
| PpsI | GAGTC | 2 cut(s) | 439, 713 |
| PpuMI | RGGWCCY | 1 cut(s) | 236 |
| Psp124BI | GAGCTC | 1 cut(s) | 867 |
| Psp5II | RGGWCCY | 1 cut(s) | 236 |
| Psp6I | CCWGG | 3 cut(s) | 208, 1122, 1275 |
| PspFI | CCCAGC | 1 cut(s) | 26 |
| PspGI | CCWGG | 3 cut(s) | 208, 1122, 1275 |
| PspN4I | GGNNCC | 5 cut(s) | 214, 398, 832, 848, 1034 |
| PspOMI | GGGCCC | 1 cut(s) | 846 |
| PspPI | GGNCC | 6 cut(s) | 213, 236, 601, 730, 846, 847 |
| PspPPI | RGGWCCY | 1 cut(s) | 236 |
| PstNI | CAGNNNCTG | 1 cut(s) | 434 |
| PsuI | RGATCY | 2 cut(s) | 199, 1032 |
| RsaI | GTAC | 4 cut(s) | 149, 1193, 1265, 1274 |
| RsaNI | GTAC | 4 cut(s) | 148, 1192, 1264, 1273 |
| RseI | CAYNNNNRTG | 3 cut(s) | 695, 1100, 1160 |
| SacI | GAGCTC | 1 cut(s) | 867 |
| SacII | CCGCGG | 1 cut(s) | 955 |
| SaqAI | TTAA | 2 cut(s) | 227, 818 |
| SatI | GCNGC | 4 cut(s) | 715, 906, 925, 955 |
| Sau3AI | GATC | 7 cut(s) | 91, 199, 462, 670, 684, 990, 1032 |
| Sau96I | GGNCC | 6 cut(s) | 213, 236, 601, 730, 846, 847 |
| SchI | GAGTC | 2 cut(s) | 439, 714 |
| ScrFI | CCNGG | 5 cut(s) | 210, 728, 729, 1124, 1277 |
| SduI | GDGCHC | 4 cut(s) | 833, 850, 867, 968 |
| SfaNI | GCATC | 2 cut(s) | 947, 1270 |
| SfcI | CTRYAG | 1 cut(s) | 841 |
| Sfr303I | CCGCGG | 1 cut(s) | 955 |
| SgrBI | CCGCGG | 1 cut(s) | 955 |
| SinI | GGWCC | 3 cut(s) | 213, 236, 601 |
| SmaI | CCCGGG | 1 cut(s) | 729 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 695, 1100, 1160 |
| SmlI | CTYRAG | 4 cut(s) | 131, 203, 226, 644 |
| SmoI | CTYRAG | 4 cut(s) | 131, 203, 226, 644 |
| Sse9I | AATT | 8 cut(s) | 45, 56, 115, 249, 376, 572, 1151, 1330 |
| SseBI | AGGCCT | 2 cut(s) | 259, 1178 |
| SsiI | CCGC | 6 cut(s) | 784, 906, 925, 942, 952, 954 |
| SspMI | CTAG | 2 cut(s) | 183, 758 |
| SstI | GAGCTC | 1 cut(s) | 867 |
| StuI | AGGCCT | 2 cut(s) | 259, 1178 |
| StyD4I | CCNGG | 5 cut(s) | 208, 726, 727, 1122, 1275 |
| StyI | CCWWGG | 1 cut(s) | 216 |
| TaaI | ACNGT | 2 cut(s) | 535, 1159 |
| TaiI | ACGT | 1 cut(s) | 1047 |
| TaqI | TCGA | 1 cut(s) | 1026 |
| TasI | AATT | 8 cut(s) | 45, 56, 115, 249, 376, 572, 1151, 1330 |
| TatI | WGTACW | 1 cut(s) | 147 |
| TauI | GCSGC | 3 cut(s) | 908, 927, 957 |
| TfiI | GAWTC | 2 cut(s) | 341, 723 |
| Tru1I | TTAA | 2 cut(s) | 227, 818 |
| Tru9I | TTAA | 2 cut(s) | 227, 818 |
| TscAI | CASTG | 4 cut(s) | 103, 420, 538, 1231 |
| TseFI | GTSAC | 2 cut(s) | 706, 1061 |
| TseI | GCWGC | 1 cut(s) | 714 |
| Tsp45I | GTSAC | 2 cut(s) | 706, 1061 |
| TspDTI | ATGAA | 6 cut(s) | 171, 557, 627, 787, 1090, 1259 |
| TspMI | CCCGGG | 1 cut(s) | 727 |
| TspRI | CASTG | 4 cut(s) | 103, 420, 538, 1231 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 415, 665, 1076 |
| Vha464I | CTTAAG | 1 cut(s) | 226 |
| VpaK11BI | GGWCC | 3 cut(s) | 213, 236, 601 |
| XapI | RAATTY | 3 cut(s) | 56, 115, 249 |
| XceI | RCATGY | 1 cut(s) | 714 |
| XmaI | CCCGGG | 1 cut(s) | 727 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1178 |
| XspI | CTAG | 2 cut(s) | 183, 758 |