FvH4_5g10192

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0019234
Physical Location & Seq
Reverse (-)
5834131 .. 5834731
601 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10192.t1

Sequence Viewer

Length: 486 bp
ATGAGGTTCAAGGCGTTCAGATGGCTTAAGGCCTTGTGGCCGGCTCAGAGATCAAGTCGGCTTCACCCGATTAATGATGGGGTTATTATGAGGAATTTCATTTCCATGAATTCCTTCGACATTATTGATTCACATGAAGATGATGATCTAGTTCCCTTTGTCTTCGAAGAAAATGAGAGGGTTGATGATGGGGTTACAAATGTGAGGACTTCCATTATCACGTTTTTGTATCACATAGCTACAAATGATGGTCTAGTTCCGCTTTCCTTCGGAGAAAATGAGAGGGATGATTCAATCTCTAGTTTTGTTCAGAGAATAAGAAGTGAGTGTTTGAAAGCTGGAGATTGTTCTATATGCTTGGGGGACTTTGAGACTATGCAGCACGGCCTCATGATCACCATATCCTTGGCTGAGGAGTCTGACGACGATTTGGGACAATGGTGTAGCCAGAAATCCCACTTAGAATGGTCAATATTTATTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

18.51

Weight (kDa)

4.57

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019234)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g10191 FvH4_5g10192
prunus_persica Prupe.5G137000_v2.0.a1
rosa_multiflora Rmu_ssc0000422.1_g000017
rosa_samantha Rh7AG092000 Rh7BG093600 Rh7CG092900 Rh7DG094100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 260
AcoI YGGCCR 1 cut(s) 38
AcsI RAATTY 2 cut(s) 94, 109
AflII CTTAAG 1 cut(s) 26
AgsI TTSAA 3 cut(s) 10, 294, 334
AluBI AGCT 2 cut(s) 239, 338
AluI AGCT 2 cut(s) 239, 338
Alw26I GTCTC 1 cut(s) 365
AoxI GGCC 3 cut(s) 30, 38, 385
ApeKI GCWGC 1 cut(s) 379
ApoI RAATTY 2 cut(s) 94, 109
AseI ATTAAT 1 cut(s) 72
Asp700I GAANNNNTTC 1 cut(s) 113
AsuHPI GGTGA 2 cut(s) 56, 388
AsuII TTCGAA 1 cut(s) 165
BbsI GAAGAC 1 cut(s) 154
BbvCI CCTCAGC 1 cut(s) 411
BbvI GCAGC 1 cut(s) 391
BccI CCATC 4 cut(s) 15, 71, 182, 242
BceAI ACGGC 1 cut(s) 400
BclI TGATCA 1 cut(s) 393
BcoDI GTCTC 1 cut(s) 365
BfaI CTAG 3 cut(s) 149, 254, 300
BfrI CTTAAG 1 cut(s) 26
BisI GCNGC 1 cut(s) 380
BlsI GCNGC 1 cut(s) 381
BpiI GAAGAC 1 cut(s) 154
BpmI CTGGAG 1 cut(s) 360
Bpu10I CCTNAGC 1 cut(s) 411
Bpu14I TTCGAA 1 cut(s) 165
BsaBI GATNNNNATC 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 405
Bse118I RCCGGY 1 cut(s) 40
Bse8I GATNNNNATC 1 cut(s) 144
BseDI CCNNGG 1 cut(s) 405
BseGI GGATG 1 cut(s) 292
BseJI GATNNNNATC 1 cut(s) 144
BseMII CTCAG 2 cut(s) 59, 402
BseRI GAGGAG 1 cut(s) 428
BseXI GCAGC 1 cut(s) 391
BshFI GGCC 3 cut(s) 32, 40, 387
BsiSI CCGG 1 cut(s) 41
BslFI GGGAC 2 cut(s) 377, 447
BsmAI GTCTC 1 cut(s) 365
BsmFI GGGAC 2 cut(s) 377, 447
BsnI GGCC 3 cut(s) 32, 40, 387
Bsp119I TTCGAA 1 cut(s) 165
Bsp143I GATC 3 cut(s) 50, 145, 393
BspACI CCGC 1 cut(s) 260
BspANI GGCC 3 cut(s) 32, 40, 387
BspCNI CTCAG 2 cut(s) 58, 403
BspHI TCATGA 1 cut(s) 390
BspT104I TTCGAA 1 cut(s) 165
BspTI CTTAAG 1 cut(s) 26
BsrFI RCCGGY 1 cut(s) 40
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 1 cut(s) 405
BssMI GATC 3 cut(s) 50, 145, 393
BssT1I CCWWGG 1 cut(s) 405
BstAFI CTTAAG 1 cut(s) 26
BstBI TTCGAA 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 3 cut(s) 45, 411, 460
BstF5I GGATG 1 cut(s) 292
BstKTI GATC 3 cut(s) 53, 148, 396
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 3 cut(s) 50, 145, 393
BstV1I GCAGC 1 cut(s) 391
BstV2I GAAGAC 1 cut(s) 154
BstXI CCANNNNNNTGG 1 cut(s) 406
BsuRI GGCC 3 cut(s) 32, 40, 387
BtsCI GGATG 1 cut(s) 292
Cac8I GCNNGC 1 cut(s) 42
CciI TCATGA 1 cut(s) 390
Cfr10I RCCGGY 1 cut(s) 40
CviAII CATG 3 cut(s) 106, 134, 391
DdeI CTNAG 3 cut(s) 45, 411, 460
DpnI GATC 3 cut(s) 52, 147, 395
DpnII GATC 3 cut(s) 50, 145, 393
EaeI YGGCCR 1 cut(s) 38
Eco130I CCWWGG 1 cut(s) 405
Eco147I AGGCCT 1 cut(s) 32
EcoRI GAATTC 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 3 cut(s) 109, 137, 394
FaiI YATR 9 cut(s) 89, 107, 135, 236, 353, 355, 377, 392, 401
FaqI GGGAC 2 cut(s) 377, 447
FatI CATG 3 cut(s) 105, 133, 390
FbaI TGATCA 1 cut(s) 393
Fnu4HI GCNGC 1 cut(s) 380
FokI GGATG 1 cut(s) 299
Fsp4HI GCNGC 1 cut(s) 380
FspBI CTAG 3 cut(s) 149, 254, 300
GluI GCNGC 1 cut(s) 380
GsuI CTGGAG 1 cut(s) 360
HaeIII GGCC 3 cut(s) 32, 40, 387
HapII CCGG 1 cut(s) 41
Hin1II CATG 3 cut(s) 109, 137, 394
HinfI GANTC 3 cut(s) 128, 290, 416
HpaII CCGG 1 cut(s) 41
HphI GGTGA 2 cut(s) 56, 388
Hpy188I TCNGA 5 cut(s) 20, 48, 272, 312, 421
Hpy188III TCNNGA 1 cut(s) 391
Hpy99I CGWCG 1 cut(s) 428
HpyAV CCTTC 2 cut(s) 124, 277
HpyCH4IV ACGT 1 cut(s) 221
HpyCH4V TGCA 1 cut(s) 379
HpyF3I CTNAG 3 cut(s) 45, 411, 460
HpySE526I ACGT 1 cut(s) 221
Hsp92II CATG 3 cut(s) 109, 137, 394
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
Ksp22I TGATCA 1 cut(s) 393
Kzo9I GATC 3 cut(s) 50, 145, 393
LpnPI CCDG 3 cut(s) 54, 324, 461
Lsp1109I GCAGC 1 cut(s) 391
MaeI CTAG 3 cut(s) 149, 254, 300
MaeII ACGT 1 cut(s) 221
MaeIII GTNAC 1 cut(s) 193
MalI GATC 3 cut(s) 52, 147, 395
MboI GATC 3 cut(s) 50, 145, 393
MboII GAAGA 3 cut(s) 149, 154, 179
MluCI AATT 2 cut(s) 94, 109
MlyI GAGTC 1 cut(s) 425
MnlI CCTC 6 cut(s) 84, 171, 198, 276, 398, 406
MroNI GCCGGC 1 cut(s) 40
MroXI GAANNNNTTC 1 cut(s) 113
MseI TTAA 2 cut(s) 27, 72
MslI CAYNNNNRTG 2 cut(s) 104, 138
MspCI CTTAAG 1 cut(s) 26
MspI CCGG 1 cut(s) 41
NaeI GCCGGC 1 cut(s) 42
NdeII GATC 3 cut(s) 50, 145, 393
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 3 cut(s) 109, 137, 394
NspV TTCGAA 1 cut(s) 165
PagI TCATGA 1 cut(s) 390
PceI AGGCCT 1 cut(s) 32
PdiI GCCGGC 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 113
PfeI GAWTC 2 cut(s) 128, 290
PkrI GCNGC 1 cut(s) 381
PleI GAGTC 1 cut(s) 424
PpsI GAGTC 1 cut(s) 424
PshBI ATTAAT 1 cut(s) 72
RseI CAYNNNNRTG 2 cut(s) 104, 138
SaqAI TTAA 2 cut(s) 27, 72
SatI GCNGC 1 cut(s) 380
Sau3AI GATC 3 cut(s) 50, 145, 393
SchI GAGTC 1 cut(s) 425
SetI ASST 4 cut(s) 8, 224, 241, 340
SfuI TTCGAA 1 cut(s) 165
SmiMI CAYNNNNRTG 2 cut(s) 104, 138
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 2 cut(s) 94, 109
SseBI AGGCCT 1 cut(s) 32
SsiI CCGC 1 cut(s) 260
SspI AATATT 1 cut(s) 474
SspMI CTAG 3 cut(s) 149, 254, 300
StuI AGGCCT 1 cut(s) 32
StyI CCWWGG 1 cut(s) 405
TaiI ACGT 1 cut(s) 224
TaqI TCGA 2 cut(s) 117, 165
TasI AATT 2 cut(s) 94, 109
TfiI GAWTC 2 cut(s) 128, 290
Tru1I TTAA 2 cut(s) 27, 72
Tru9I TTAA 2 cut(s) 27, 72
TseI GCWGC 1 cut(s) 379
TspDTI ATGAA 3 cut(s) 88, 122, 150
Vha464I CTTAAG 1 cut(s) 26
VspI ATTAAT 1 cut(s) 72
XapI RAATTY 2 cut(s) 94, 109
XmnI GAANNNNTTC 1 cut(s) 113
XspI CTAG 3 cut(s) 149, 254, 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.