Prupe.5G137000_v2.0.a1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0019234
Physical Location & Seq
Forward (+)
12934743 .. 12935339
597 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G137000.1

Sequence Viewer

Length: 564 bp
ATGCAAACAAATCATCCAATTCCCGTCGAAGATCAGTATCATCTCAAATTCAAACTCTTCAAGGAAACCGAAACGGTTTGGACAGACGAAGACGACGTTCCAGTCGAAGTCAGTCGTGAGCCGCCGCTCTTTGACTCCGAAATCGACATTACGCTCTCTCGCGCCGATTTATTATTAGTATTTGAAGAAAGTCATAGCACAAATTCCGCTTCCGATTCAATTAGCCTGATGGCCGACGCCCTGGCCGCCATGGAAATCCCGGAGTTCAAACACGAGCACATCATTGGGTCTTTATTGGCTGGTTTTAAGGCGAAGAAGTCGGAATGTTTGCCCCATGTGAGAATGGAACGATCGCAGATTAGGGGAGAGACGATGTGTGCGATTTGTAGGGAGGAATTTGAGAGCGGTACGAATGGGAATCGGCTTCTTCACTTGCCCTGCTCTCATATTTTTCATGTCAACTGCATCGAGAAGTGGCTCGAAACCTGTCCTAGTCATCGCTCGTGCCCCTTGTGCCGTTTCCCACTGTGTCGTCGTTTTGATTATCAATTAGTTTTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

188

Amino Acids

21.57

Weight (kDa)

5.33

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019234)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g10191 FvH4_5g10192
prunus_persica Prupe.5G137000_v2.0.a1
rosa_multiflora Rmu_ssc0000422.1_g000017
rosa_samantha Rh7AG092000 Rh7BG093600 Rh7CG092900 Rh7DG094100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 101
AccBSI CCGCTC 2 cut(s) 127, 405
AccII CGCG 1 cut(s) 162
AciI CCGC 5 cut(s) 122, 125, 207, 246, 405
AcoI YGGCCR 2 cut(s) 231, 243
AcsI RAATTY 3 cut(s) 47, 202, 395
AcyI GRCGYC 1 cut(s) 237
AfaI GTAC 1 cut(s) 409
AgsI TTSAA 5 cut(s) 52, 61, 185, 219, 268
AjnI CCWGG 1 cut(s) 240
Alw21I GWGCWC 1 cut(s) 279
Alw26I GTCTC 1 cut(s) 362
AoxI GGCC 2 cut(s) 231, 243
ApoI RAATTY 3 cut(s) 47, 202, 395
AspLEI GCGC 1 cut(s) 164
AsuC2I CCSGG 1 cut(s) 260
BaeGI GKGCMC 1 cut(s) 509
BauI CACGAG 2 cut(s) 272, 502
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 279
BccI CCATC 1 cut(s) 223
BceAI ACGGC 1 cut(s) 501
BciT130I CCWGG 1 cut(s) 242
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 1 cut(s) 362
BfaI CTAG 1 cut(s) 492
BisI GCNGC 3 cut(s) 122, 125, 246
BlsI GCNGC 3 cut(s) 123, 126, 247
Bme1390I CCNGG 2 cut(s) 242, 260
BmrFI CCNGG 2 cut(s) 242, 260
BmsI GCATC 1 cut(s) 474
BpiI GAAGAC 1 cut(s) 96
BpuMI CCSGG 1 cut(s) 260
BsaBI GATNNNNATC 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 2 cut(s) 240, 249
Bse1I ACTGG 1 cut(s) 101
Bse8I GATNNNNATC 1 cut(s) 36
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 2 cut(s) 240, 249
BseGI GGATG 1 cut(s) 13
BseJI GATNNNNATC 1 cut(s) 36
BseNI ACTGG 1 cut(s) 101
BseSI GKGCMC 1 cut(s) 509
Bsh1236I CGCG 1 cut(s) 162
Bsh1285I CGRYCG 1 cut(s) 353
BshFI GGCC 2 cut(s) 233, 245
BsiEI CGRYCG 1 cut(s) 353
BsiHKAI GWGCWC 1 cut(s) 279
BsiSI CCGG 1 cut(s) 260
BsmAI GTCTC 1 cut(s) 362
BsmBI CGTCTC 1 cut(s) 362
BsnI GGCC 2 cut(s) 233, 245
Bsp1286I GDGCHC 2 cut(s) 279, 509
Bsp143I GATC 2 cut(s) 31, 350
Bsp19I CCATGG 1 cut(s) 249
BspACI CCGC 5 cut(s) 122, 125, 207, 246, 405
BspANI GGCC 2 cut(s) 233, 245
BspFNI CGCG 1 cut(s) 162
BsrBI CCGCTC 2 cut(s) 127, 405
BsrI ACTGG 1 cut(s) 101
BssECI CCNNGG 2 cut(s) 240, 249
BssMI GATC 2 cut(s) 31, 350
BssNI GRCGYC 1 cut(s) 237
BssSI CACGAG 2 cut(s) 272, 502
BssT1I CCWWGG 1 cut(s) 249
Bst2BI CACGAG 2 cut(s) 272, 502
Bst2UI CCWGG 1 cut(s) 242
Bst4CI ACNGT 2 cut(s) 76, 528
Bst6I CTCTTC 1 cut(s) 62
BstACI GRCGYC 1 cut(s) 237
BstDSI CCRYGG 1 cut(s) 249
BstF5I GGATG 1 cut(s) 13
BstFNI CGCG 1 cut(s) 162
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 2 cut(s) 34, 353
BstMAI GTCTC 1 cut(s) 362
BstMBI GATC 2 cut(s) 31, 350
BstMCI CGRYCG 1 cut(s) 353
BstMWI GCNNNNNNNGC 2 cut(s) 245, 513
BstNI CCWGG 1 cut(s) 242
BstSCI CCNGG 2 cut(s) 240, 258
BstSLI GKGCMC 1 cut(s) 509
BstUI CGCG 1 cut(s) 162
BstV2I GAAGAC 1 cut(s) 96
BsuRI GGCC 2 cut(s) 233, 245
BtgI CCRYGG 1 cut(s) 249
BtgZI GCGATG 1 cut(s) 482
BtsCI GGATG 1 cut(s) 13
BtsIMutI CAGTG 1 cut(s) 524
CfoI GCGC 1 cut(s) 164
CseI GACGC 1 cut(s) 245
Csp6I GTAC 1 cut(s) 408
CviAII CATG 3 cut(s) 250, 335, 455
CviJI RGCY 7 cut(s) 121, 225, 233, 245, 299, 424, 478
CviKI_1 RGCY 7 cut(s) 121, 225, 233, 245, 299, 424, 478
CviQI GTAC 1 cut(s) 408
DpnI GATC 2 cut(s) 33, 352
DpnII GATC 2 cut(s) 31, 350
DrdI GACNNNNNNGTC 1 cut(s) 101
DseDI GACNNNNNNGTC 1 cut(s) 101
EaeI YGGCCR 2 cut(s) 231, 243
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco130I CCWWGG 1 cut(s) 249
EcoRII CCWGG 1 cut(s) 240
EcoT14I CCWWGG 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 249
Esp3I CGTCTC 1 cut(s) 362
FaeI CATG 3 cut(s) 253, 338, 458
FaiI YATR 6 cut(s) 195, 251, 336, 447, 456, 560
FatI CATG 3 cut(s) 249, 334, 454
Fnu4HI GCNGC 3 cut(s) 122, 125, 246
Fsp4HI GCNGC 3 cut(s) 122, 125, 246
FspBI CTAG 1 cut(s) 492
GlaI GCGC 1 cut(s) 163
GluI GCNGC 3 cut(s) 122, 125, 246
HaeIII GGCC 2 cut(s) 233, 245
HapII CCGG 1 cut(s) 260
HgaI GACGC 1 cut(s) 245
HhaI GCGC 1 cut(s) 164
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 3 cut(s) 253, 338, 458
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HinfI GANTC 3 cut(s) 134, 215, 418
HpaII CCGG 1 cut(s) 260
Hpy166II GTNNAC 1 cut(s) 460
Hpy188I TCNGA 3 cut(s) 139, 214, 322
Hpy188III TCNNGA 2 cut(s) 116, 469
Hpy8I GTNNAC 1 cut(s) 460
Hpy99I CGWCG 4 cut(s) 29, 98, 239, 537
HpyCH4III ACNGT 2 cut(s) 76, 528
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 2 cut(s) 4, 465
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 513
HpySE526I ACGT 1 cut(s) 96
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 3 cut(s) 253, 338, 458
HspAI GCGC 1 cut(s) 162
Kzo9I GATC 2 cut(s) 31, 350
LpnPI CCDG 8 cut(s) 114, 227, 239, 254, 273, 285, 451, 499
LweI GCATC 1 cut(s) 474
MaeI CTAG 1 cut(s) 492
MaeII ACGT 1 cut(s) 96
MalI GATC 2 cut(s) 33, 352
MbiI CCGCTC 2 cut(s) 127, 405
MboI GATC 2 cut(s) 31, 350
MboII GAAGA 6 cut(s) 41, 49, 101, 197, 325, 419
MhlI GDGCHC 2 cut(s) 279, 509
MluCI AATT 6 cut(s) 18, 47, 202, 219, 395, 548
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 1 cut(s) 385
MseI TTAA 1 cut(s) 306
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 2 cut(s) 242, 260
MvaI CCWGG 1 cut(s) 242
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 2 cut(s) 245, 513
NciI CCSGG 1 cut(s) 260
NcoI CCATGG 1 cut(s) 249
NdeII GATC 2 cut(s) 31, 350
NlaIII CATG 3 cut(s) 253, 338, 458
PcsI WCGNNNNNNNCGW 3 cut(s) 93, 102, 377
PfeI GAWTC 2 cut(s) 215, 418
PfoI TCCNGGA 1 cut(s) 258
PkrI GCNGC 3 cut(s) 123, 126, 247
Ple19I CGATCG 1 cut(s) 353
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PvuI CGATCG 1 cut(s) 353
RsaI GTAC 1 cut(s) 409
RsaNI GTAC 1 cut(s) 408
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 3 cut(s) 122, 125, 246
Sau3AI GATC 2 cut(s) 31, 350
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 2 cut(s) 242, 260
SduI GDGCHC 2 cut(s) 279, 509
SetI ASST 2 cut(s) 99, 488
SfaNI GCATC 1 cut(s) 474
Sse9I AATT 6 cut(s) 18, 47, 202, 219, 395, 548
SsiI CCGC 5 cut(s) 122, 125, 207, 246, 405
SspMI CTAG 1 cut(s) 492
StyD4I CCNGG 2 cut(s) 240, 258
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 2 cut(s) 76, 528
TaiI ACGT 1 cut(s) 99
TaqI TCGA 5 cut(s) 27, 105, 144, 468, 480
TasI AATT 6 cut(s) 18, 47, 202, 219, 395, 548
TauI GCSGC 3 cut(s) 124, 127, 248
TfiI GAWTC 2 cut(s) 215, 418
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TscAI CASTG 1 cut(s) 531
TspDTI ATGAA 1 cut(s) 443
TspRI CASTG 1 cut(s) 531
XapI RAATTY 3 cut(s) 47, 202, 395
XspI CTAG 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.