FvH4_5g10810

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0018038
Physical Location & Seq
Reverse (-)
6111482 .. 6114293
2812 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10810.t1

Sequence Viewer

Length: 1491 bp
ATGGCTTCTGCTGCCACATTTCCCTTAGCGACACAAGTTCTATGCTTGAGTCTTTGTTTTCTTAGCTTAGGTTGCTCTGCCTTCACTGTTGAAGATTACTCGGATGCTCTTGACAAGTCCATTCTCTTCTTCGAGGGCCAGAGGTCCGGCAAGTTGCCATCCAACCAGCGGCTCAATTGGAGGGGCAATTCTGGCTTGTCTGATGGCTCCTCTTATCATGTGGACTTAGTAGGCGGCTACTATGATGCCGGAGACAATGTCAAGTTTGGGCTACCAATGGCCTTCACTACTACACTGTTAGCATGGAGTGTTATCGAGTTTGGTAGCTCTATGCCTAATCAGATTGACAATGCAAAGGCTGCGATACGTTGGAGCACAGACTATCTGCTCAAGGCAGCCACCACAACCCCGGGAACCTTATATGTCCAAGTGGCAGATCCAAACATGGACCATCACTGCTGGGAGAGGCCAGAAGATATGGATACACCGCGCAATGTTTACAAGGTGTCCACTCAAAATCCAGGGTCTGATGTAGCAGCAGAAACTGCTGCTGCACTGGCTGCAGCTTCCATAGTCTTCAAAGACTCTGACCCTTCTTACTCTGGAAAATTGCTTCACACAGCAATGAAAGTGTTTGATTTTGCAGACAAGTACAGAGGTTCTTACAGTGACTCCCTCGGCTCTGTGGTCTGTCCTTTTTACTGCTCATATTCTGGATACCAAGATGAACTTCTTTGGGGCGCATCATGGATTCACAGAGCCTCGGAGAACAGTTCATACTTGTCATACATTAAGTCCAATGGCCATACATTGGGTGCTGATGATGATGACTACTCCTTCAGTTGGGATGACAAGCGAGCTGGAACAAAAGTCCTCCTTTCCAAGAGTTTTCTAGAGACAAACACTGAAGAATTTCAGATATACAAAGCACACTCAGACAACTACATATGCTCTCTAATTCCAGGATCATCTAGTTTTCAGGCTCAATATACCCCTGGTGGACTTCTGTACAAGGCAAGTGAGAGCAATCTCCAGTATGTTACTTCCACAACACTCCTCCTCCTTACATATGCAAAGTACCTCAGTTCAAACAGCTTAGCAGTCACATGTGGATCCCTAGCCGTCACAGGCAAAACCCTAATCGCGCAGGCAAAGAAGCAAGTGGACTACATTCTTGGTGACAATCCTGCAAAGATGTCATACATGGTGGGATTTGGGAAGAAGTATCCACAGCATGTACACCACAGGGGTTCTTCTGTGCCATCTGTACACAATCACCCTAACCCCATTCCCTGCAACGATGGATTTCAGTACTTCCACTCTGGTTCCCCAAATCCAAATGTGCTCGTCGGAGCCATAGTTGGCGGACCTGATAACAAAGACAACTTTGCCGATGACCGGAACAACTACCAGCAATCCGAGCCTGCTACTTACATCAACGCACCATTTGTTGGGGCTCTTGCTTTCTTCTCTGCCAACACCAACCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

497

Amino Acids

54.07

Weight (kDa)

5.5

Isoelectric Point (pI)

27.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 33 - 487 9.3e-165 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018038)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g10810
malus_domestica MD06G1120700.v1.1
prunus_persica Prupe.5G131300_v2.0.a1
pyrus_communis pycom06g11200
rosa_chinensis RchiOBHm_Chr7g0187351
rosa_laevigata RLG00000004792
rosa_multiflora Rmu_sc0001758.1_g000011
rosa_roxburghii Rroxscaffold_3G00267570
rosa_rugosa Rorug06G0480800
rosa_samantha Rh7CG086300
rosa_wichuraiana Rw7G007500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 811, 1451
AccII CGCG 2 cut(s) 490, 1145
AciI CCGC 4 cut(s) 169, 234, 488, 1365
AclWI GGATC 4 cut(s) 431, 973, 1107, 1120
AcoI YGGCCR 1 cut(s) 802
AcsI RAATTY 1 cut(s) 911
AcuI CTGAAG 2 cut(s) 823, 927
AfaI GTAC 6 cut(s) 653, 1010, 1079, 1239, 1269, 1313
AfiI CCNNNNNNNGG 5 cut(s) 168, 811, 843, 1398, 1451
AflIII ACRYGT 1 cut(s) 1106
AgsI TTSAA 3 cut(s) 92, 580, 1089
AjnI CCWGG 3 cut(s) 520, 961, 994
AjuI GAANNNNNNNTTGG 2 cut(s) 714, 746
AluBI AGCT 5 cut(s) 66, 327, 566, 860, 1095
AluI AGCT 5 cut(s) 66, 327, 566, 860, 1095
Alw21I GWGCWC 2 cut(s) 377, 1347
Alw26I GTCTC 2 cut(s) 246, 890
AlwI GGATC 4 cut(s) 431, 973, 1107, 1120
AlwNI CAGNNNCTG 2 cut(s) 527, 545
Ama87I CYCGRG 1 cut(s) 409
AoxI GGCC 4 cut(s) 136, 279, 467, 802
ApeKI GCWGC 8 cut(s) 11, 359, 395, 536, 548, 551, 560, 563
ApoI RAATTY 1 cut(s) 911
Asp700I GAANNNNTTC 1 cut(s) 912
AspLEI GCGC 3 cut(s) 492, 743, 1147
AspS9I GGNCC 4 cut(s) 136, 144, 448, 1367
AsuC2I CCSGG 2 cut(s) 410, 411
AsuHPI GGTGA 2 cut(s) 1190, 1268
AvaI CYCGRG 1 cut(s) 409
AvaII GGWCC 3 cut(s) 144, 448, 1367
BalI TGGCCA 1 cut(s) 804
BamHI GGATCC 1 cut(s) 1112
BanII GRGCYC 1 cut(s) 1459
BbsI GAAGAC 1 cut(s) 568
Bbv12I GWGCWC 2 cut(s) 377, 1347
BbvI GCAGC 7 cut(s) 346, 407, 535, 538, 547, 548, 575
BccI CCATC 5 cut(s) 166, 197, 459, 1270, 1295
BceAI ACGGC 1 cut(s) 1106
BciT130I CCWGG 3 cut(s) 522, 963, 996
BciVI GTATCC 3 cut(s) 475, 710, 1236
BcnI CCSGG 2 cut(s) 410, 411
BcoDI GTCTC 2 cut(s) 246, 890
BfaI CTAG 3 cut(s) 893, 972, 1118
BfmI CTRYAG 1 cut(s) 561
BfuI GTATCC 3 cut(s) 475, 710, 1236
BlpI GCTNAGC 1 cut(s) 1096
BmcAI AGTACT 1 cut(s) 1313
Bme1390I CCNGG 5 cut(s) 410, 411, 522, 963, 996
Bme18I GGWCC 3 cut(s) 144, 448, 1367
BmeT110I CYCGRG 1 cut(s) 409
BmgT120I GGNCC 4 cut(s) 136, 144, 448, 1367
BmiI GGNNCC 5 cut(s) 208, 415, 1114, 1327, 1354
BmrFI CCNGG 5 cut(s) 410, 411, 522, 963, 996
BmsI GCATC 3 cut(s) 94, 235, 752
BpiI GAAGAC 1 cut(s) 568
BpmI CTGGAG 1 cut(s) 1016
Bpu10I CCTNAGC 2 cut(s) 25, 67
Bpu1102I GCTNAGC 1 cut(s) 1096
BpuEI CTTGAG 2 cut(s) 67, 374
BpuMI CCSGG 2 cut(s) 410, 411
BsaJI CCNNGG 6 cut(s) 408, 409, 521, 676, 762, 994
BsaWI WCCGGW 1 cut(s) 1398
BsaXI ACNNNNNCTCC 8 cut(s) 243, 273, 656, 686, 1044, 1074, 1344, 1374
Bsc4I CCNNNNNNNGG 5 cut(s) 168, 811, 843, 1398, 1451
Bse1I ACTGG 2 cut(s) 561, 1033
Bse3DI GCAATG 2 cut(s) 499, 630
BseBI CCWGG 3 cut(s) 522, 963, 996
BseDI CCNNGG 6 cut(s) 408, 409, 521, 676, 762, 994
BseGI GGATG 3 cut(s) 109, 158, 853
BseLI CCNNNNNNNGG 5 cut(s) 168, 811, 843, 1398, 1451
BseMI GCAATG 2 cut(s) 499, 630
BseMII CTCAG 2 cut(s) 948, 1096
BseNI ACTGG 2 cut(s) 561, 1033
BseRI GAGGAG 3 cut(s) 199, 1046, 1049
BseXI GCAGC 7 cut(s) 346, 407, 535, 538, 547, 548, 575
BseYI CCCAGC 1 cut(s) 459
BsgI GTGCAG 1 cut(s) 537
Bsh1236I CGCG 2 cut(s) 490, 1145
BshFI GGCC 4 cut(s) 138, 281, 469, 804
BsiHKAI GWGCWC 2 cut(s) 377, 1347
BsiHKCI CYCGRG 1 cut(s) 409
BsiSI CCGG 4 cut(s) 147, 249, 410, 1399
BslI CCNNNNNNNGG 5 cut(s) 168, 811, 843, 1398, 1451
BsmAI GTCTC 2 cut(s) 246, 890
BsnI GGCC 4 cut(s) 138, 281, 469, 804
BsoBI CYCGRG 1 cut(s) 409
Bsp1286I GDGCHC 3 cut(s) 377, 1347, 1459
Bsp1407I TGTACA 3 cut(s) 1008, 1237, 1267
Bsp143I GATC 3 cut(s) 436, 965, 1112
Bsp1720I GCTNAGC 1 cut(s) 1096
BspACI CCGC 4 cut(s) 169, 234, 488, 1365
BspANI GGCC 4 cut(s) 138, 281, 469, 804
BspCNI CTCAG 2 cut(s) 947, 1095
BspFNI CGCG 2 cut(s) 490, 1145
BspLI GGNNCC 5 cut(s) 208, 415, 1114, 1327, 1354
BspMAI CTGCAG 1 cut(s) 565
BspPI GGATC 4 cut(s) 431, 973, 1107, 1120
BsrDI GCAATG 2 cut(s) 499, 630
BsrGI TGTACA 3 cut(s) 1008, 1237, 1267
BsrI ACTGG 2 cut(s) 561, 1033
BssECI CCNNGG 6 cut(s) 408, 409, 521, 676, 762, 994
BssMI GATC 3 cut(s) 436, 965, 1112
Bst2UI CCWGG 3 cut(s) 522, 963, 996
Bst4CI ACNGT 4 cut(s) 88, 297, 668, 773
Bst6I CTCTTC 1 cut(s) 131
BstAPI GCANNNNNTGC 3 cut(s) 359, 545, 560
BstAUI TGTACA 3 cut(s) 1008, 1237, 1267
BstC8I GCNNGC 3 cut(s) 858, 1149, 1425
BstDEI CTNAG 7 cut(s) 25, 62, 67, 226, 934, 1082, 1096
BstF5I GGATG 3 cut(s) 109, 158, 853
BstFNI CGCG 2 cut(s) 490, 1145
BstHHI GCGC 3 cut(s) 492, 743, 1147
BstKTI GATC 3 cut(s) 439, 968, 1115
BstMAI GTCTC 2 cut(s) 246, 890
BstMBI GATC 3 cut(s) 436, 965, 1112
BstMWI GCNNNNNNNGC 8 cut(s) 11, 72, 192, 359, 545, 557, 560, 1420
BstNI CCWGG 3 cut(s) 522, 963, 996
BstNSI RCATGY 2 cut(s) 1110, 1238
BstSCI CCNGG 5 cut(s) 408, 409, 520, 961, 994
BstSFI CTRYAG 1 cut(s) 561
BstUI CGCG 2 cut(s) 490, 1145
BstV1I GCAGC 7 cut(s) 346, 407, 535, 538, 547, 548, 575
BstV2I GAAGAC 1 cut(s) 568
BstX2I RGATCY 2 cut(s) 436, 1112
BstYI RGATCY 2 cut(s) 436, 1112
BsuI GTATCC 3 cut(s) 475, 710, 1236
BsuRI GGCC 4 cut(s) 138, 281, 469, 804
BtsCI GGATG 3 cut(s) 109, 158, 853
BtsI GCAGTG 1 cut(s) 454
BtsIMutI CAGTG 6 cut(s) 84, 293, 454, 554, 673, 903
Cac8I GCNNGC 3 cut(s) 858, 1149, 1425
CaiI CAGNNNCTG 2 cut(s) 527, 545
CfoI GCGC 3 cut(s) 492, 743, 1147
Cfr13I GGNCC 4 cut(s) 136, 144, 448, 1367
Cfr9I CCCGGG 1 cut(s) 409
Csp6I GTAC 6 cut(s) 652, 1009, 1078, 1238, 1268, 1312
CviAII CATG 7 cut(s) 218, 303, 445, 747, 1107, 1204, 1235
CviQI GTAC 6 cut(s) 652, 1009, 1078, 1238, 1268, 1312
DdeI CTNAG 7 cut(s) 25, 62, 67, 226, 934, 1082, 1096
DpnI GATC 3 cut(s) 438, 967, 1114
DpnII GATC 3 cut(s) 436, 965, 1112
EaeI YGGCCR 1 cut(s) 802
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
EciI GGCGGA 1 cut(s) 1380
Eco24I GRGCYC 1 cut(s) 1459
Eco47I GGWCC 3 cut(s) 144, 448, 1367
Eco57I CTGAAG 2 cut(s) 823, 927
Eco88I CYCGRG 1 cut(s) 409
EcoRII CCWGG 3 cut(s) 520, 961, 994
EcoT38I GRGCYC 1 cut(s) 1459
FaeI CATG 7 cut(s) 221, 306, 448, 750, 1110, 1207, 1238
FalI AAGNNNNNCTT 4 cut(s) 714, 746, 861, 893
FatI CATG 7 cut(s) 217, 302, 444, 746, 1106, 1203, 1234
FauNDI CATATG 2 cut(s) 947, 1069
FokI GGATG 3 cut(s) 116, 145, 860
FriOI GRGCYC 1 cut(s) 1459
FspBI CTAG 3 cut(s) 893, 972, 1118
GlaI GCGC 3 cut(s) 491, 742, 1146
GsaI CCCAGC 1 cut(s) 463
GsuI CTGGAG 1 cut(s) 1016
HaeIII GGCC 4 cut(s) 138, 281, 469, 804
HapII CCGG 4 cut(s) 147, 249, 410, 1399
HhaI GCGC 3 cut(s) 492, 743, 1147
Hin1II CATG 7 cut(s) 221, 306, 448, 750, 1110, 1207, 1238
Hin6I GCGC 3 cut(s) 490, 741, 1145
HinP1I GCGC 3 cut(s) 490, 741, 1145
HinfI GANTC 4 cut(s) 49, 584, 671, 751
HpaII CCGG 4 cut(s) 147, 249, 410, 1399
HphI GGTGA 2 cut(s) 1190, 1268
Hpy166II GTNNAC 7 cut(s) 223, 499, 510, 1001, 1165, 1240, 1270
Hpy188III TCNNGA 4 cut(s) 110, 603, 714, 893
Hpy8I GTNNAC 7 cut(s) 223, 499, 510, 1001, 1165, 1240, 1270
Hpy99I CGWCG 1 cut(s) 1352
HpyAV CCTTC 4 cut(s) 91, 292, 603, 847
HpyCH4III ACNGT 4 cut(s) 88, 297, 668, 773
HpyCH4IV ACGT 1 cut(s) 367
HpyCH4V TGCA 7 cut(s) 353, 554, 563, 644, 1073, 1190, 1296
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 72, 192, 359, 545, 557, 560, 1420
HpyF3I CTNAG 7 cut(s) 25, 62, 67, 226, 934, 1082, 1096
HpySE526I ACGT 1 cut(s) 367
Hsp92II CATG 7 cut(s) 221, 306, 448, 750, 1110, 1207, 1238
HspAI GCGC 3 cut(s) 490, 741, 1145
Kzo9I GATC 3 cut(s) 436, 965, 1112
LmnI GCTCC 3 cut(s) 212, 372, 1352
Lsp1109I GCAGC 7 cut(s) 346, 407, 535, 538, 547, 548, 575
LweI GCATC 3 cut(s) 94, 235, 752
MaeI CTAG 3 cut(s) 893, 972, 1118
MaeII ACGT 1 cut(s) 367
MaeIII GTNAC 5 cut(s) 668, 1039, 1102, 1123, 1178
MalI GATC 3 cut(s) 438, 967, 1114
MboI GATC 3 cut(s) 436, 965, 1112
MboII GAAGA 9 cut(s) 104, 118, 121, 485, 568, 920, 1231, 1245, 1459
MfeI CAATTG 1 cut(s) 175
MflI RGATCY 2 cut(s) 436, 1112
MhlI GDGCHC 3 cut(s) 377, 1347, 1459
MlsI TGGCCA 1 cut(s) 804
MluCI AATT 5 cut(s) 175, 187, 608, 911, 957
MluNI TGGCCA 1 cut(s) 804
MlyI GAGTC 3 cut(s) 58, 578, 665
MmeI TCCRAC 3 cut(s) 186, 350, 1330
Mox20I TGGCCA 1 cut(s) 804
MroXI GAANNNNTTC 1 cut(s) 912
MscI TGGCCA 1 cut(s) 804
MseI TTAA 1 cut(s) 792
MslI CAYNNNNRTG 1 cut(s) 623
Msp20I TGGCCA 1 cut(s) 804
MspA1I CMGCKG 1 cut(s) 169
MspI CCGG 4 cut(s) 147, 249, 410, 1399
MspR9I CCNGG 5 cut(s) 410, 411, 522, 963, 996
MunI CAATTG 1 cut(s) 175
MvaI CCWGG 3 cut(s) 522, 963, 996
MvnI CGCG 2 cut(s) 490, 1145
MwoI GCNNNNNNNGC 8 cut(s) 11, 72, 192, 359, 545, 557, 560, 1420
NciI CCSGG 2 cut(s) 410, 411
NdeI CATATG 2 cut(s) 947, 1069
NdeII GATC 3 cut(s) 436, 965, 1112
NlaIII CATG 7 cut(s) 221, 306, 448, 750, 1110, 1207, 1238
NlaIV GGNNCC 5 cut(s) 208, 415, 1114, 1327, 1354
NmeAIII GCCGAG 1 cut(s) 657
NmuCI GTSAC 4 cut(s) 668, 1102, 1123, 1178
NspI RCATGY 2 cut(s) 1110, 1238
PciI ACATGT 1 cut(s) 1106
PdmI GAANNNNTTC 1 cut(s) 912
PfeI GAWTC 1 cut(s) 751
PflFI GACNNNGTC 1 cut(s) 257
PflMI CCANNNNNTGG 2 cut(s) 811, 1451
PfoI TCCNGGA 1 cut(s) 961
PleI GAGTC 3 cut(s) 57, 578, 665
PpsI GAGTC 3 cut(s) 57, 578, 665
PscI ACATGT 1 cut(s) 1106
Psp6I CCWGG 3 cut(s) 520, 961, 994
PspFI CCCAGC 1 cut(s) 459
PspGI CCWGG 3 cut(s) 520, 961, 994
PspN4I GGNNCC 5 cut(s) 208, 415, 1114, 1327, 1354
PspPI GGNCC 4 cut(s) 136, 144, 448, 1367
PsrI GAACNNNNNNTAC 2 cut(s) 761, 793
PstI CTGCAG 1 cut(s) 565
PstNI CAGNNNCTG 2 cut(s) 527, 545
PsuI RGATCY 2 cut(s) 436, 1112
PsyI GACNNNGTC 1 cut(s) 257
RsaI GTAC 6 cut(s) 653, 1010, 1079, 1239, 1269, 1313
RsaNI GTAC 6 cut(s) 652, 1009, 1078, 1238, 1268, 1312
RseI CAYNNNNRTG 1 cut(s) 623
SaqAI TTAA 1 cut(s) 792
Sau3AI GATC 3 cut(s) 436, 965, 1112
Sau96I GGNCC 4 cut(s) 136, 144, 448, 1367
ScaI AGTACT 1 cut(s) 1313
SchI GAGTC 3 cut(s) 58, 578, 665
ScrFI CCNGG 5 cut(s) 410, 411, 522, 963, 996
SduI GDGCHC 3 cut(s) 377, 1347, 1459
SfaNI GCATC 3 cut(s) 94, 235, 752
SfcI CTRYAG 1 cut(s) 561
SinI GGWCC 3 cut(s) 144, 448, 1367
SmaI CCCGGG 1 cut(s) 411
SmiMI CAYNNNNRTG 1 cut(s) 623
SmlI CTYRAG 2 cut(s) 46, 389
SmoI CTYRAG 2 cut(s) 46, 389
Sse9I AATT 5 cut(s) 175, 187, 608, 911, 957
SsiI CCGC 4 cut(s) 169, 234, 488, 1365
SspMI CTAG 3 cut(s) 893, 972, 1118
StyD4I CCNGG 5 cut(s) 408, 409, 520, 961, 994
TaaI ACNGT 4 cut(s) 88, 297, 668, 773
TaiI ACGT 1 cut(s) 370
TaqI TCGA 2 cut(s) 132, 315
TasI AATT 5 cut(s) 175, 187, 608, 911, 957
TatI WGTACW 5 cut(s) 651, 1008, 1237, 1267, 1311
TauI GCSGC 2 cut(s) 172, 237
TfiI GAWTC 1 cut(s) 751
Tru1I TTAA 1 cut(s) 792
Tru9I TTAA 1 cut(s) 792
TscAI CASTG 6 cut(s) 91, 300, 461, 561, 673, 910
TseFI GTSAC 4 cut(s) 668, 1102, 1123, 1178
TseI GCWGC 8 cut(s) 11, 359, 395, 536, 548, 551, 560, 563
Tsp45I GTSAC 4 cut(s) 668, 1102, 1123, 1178
TspDTI ATGAA 3 cut(s) 641, 741, 765
TspMI CCCGGG 1 cut(s) 409
TspRI CASTG 6 cut(s) 91, 300, 461, 561, 673, 910
Tth111I GACNNNGTC 1 cut(s) 257
Van91I CCANNNNNTGG 2 cut(s) 811, 1451
VpaK11BI GGWCC 3 cut(s) 144, 448, 1367
XapI RAATTY 1 cut(s) 911
XbaI TCTAGA 1 cut(s) 892
XceI RCATGY 2 cut(s) 1110, 1238
XmaI CCCGGG 1 cut(s) 409
XmnI GAANNNNTTC 1 cut(s) 912
XspI CTAG 3 cut(s) 893, 972, 1118
ZrmI AGTACT 1 cut(s) 1313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.