RchiOBHm_Chr7g0187351

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0018038
Physical Location & Seq
Forward (+)
7128684 .. 7131283
2600 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16722

Sequence Viewer

Length: 1494 bp
ATGGCTTCTGCTGCCACATTTTCCTTAGTGACACAACTTCTATGCTTGACTCTTTGTTTACTTAGCTTAGGCTGCTCTGCCTTCACTACTGAAGATTACTCAGATGCTCTTGACAAGTCCATTCTCTTCTTCGAGGGCCAGAGGTCTGGCAAGTTGCCATCAAACCAGAGGCTCAATTGGAGGGGCAATTCTGGCATGTCTGATGGCTCCTCTTATCATGTGGATTTAGTAGGCGGCTACTATGATGCTGGAGATAATGTCAAGTTTGGGCTACCAATGGCCTTCACTACTACATTGTTAGCATGGAGTGTTATTGAGTTTGGTAGCTCTATGCCTAACCAGATTGAAAATGCCAAGGCTGCCGTACGGTGGAGCACAGATTATCTGTTAAAGGCAGCCACCACAACCCCAGGAACCTTATATGTCCAAGTGGCAGATCCAAACATGGACCACAGCTGCTGGGAGAGGCCAGAAGATATGGATACACCGCGCAATGTTTACAAGGTGTCAACTCAAAATCCAGGGTCCGATGTTGCAGCAGAAACTGCTGCTGCACTGGCTGCAGCTTCCATAGTCTTCAAGGACTCTGACCCTTCTTACTCCGGAAAATTGCTTCACACAGCTATGGAAGTGTTTGATTTTGCAGACAAGTACAGAGGTTCTTACAGTGACTCCCTCGGCTCAGTGGTCTGTCCTTTTTACTGCTCATATTCCGGATACCAAGATGAGCTTCTTTGGGGCGCGTCCTGGATTCATAGAGCCTCGAAGAACAGTTCATACTTGTCATACATCAAGTCCAATGGCCACACATTGGGTGCTGATGATGATGACTACTCTTTTAGTTGGGATGACAAGCGAGCCGGAACAAAGGTCCTCCTTTCCAAGAGCTTTCTAGAGACCAACACTGAAGAATTCCAGATATACAAAGCACACTCAGACAACTACATATGCTCTCTAATTCCAGGATCATCTAGTTTTCAGGCCCAATATACCCCTGGAGGACTTTTGTACAAAGCAAGTGAGAGCAATCTCCAGTATGTTACTTCCACAACACTCCTCCTCCTCACATATGCAAAGTACCTTAGTTCAAACAGAGTAGCAGTAACATGTGGATCATCAGCTGTCACAGCCGAAACCCTAATCGCACAGGCAAAGAAGCAAGTGGACTACATCCTTGGTGACAATCCGGCAAAGATGTCATACATGGTGGGATTTGGGAAGAAGTATCCACAGCATGTACACCACAGGGGTTCTTCTGTGCCATCTGTACATGAACACCCAGGCCGCATTTCCTGCAACGATGGATTTCAGTACTTCCACTCTGGTTCCCCAAATCCAAATGTGCTTGTTGGGGCCATAGTTGGCGGACCTGACAACAAAGACAACTTCGCGGATGACCGGAACAACTACCAGCAATCCGAGCCTGCCACTTACATCAATGCACCATTTGTTGGGGCTCTTGCTTTCTTCTCTGCCAACACCAACCCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

497

Amino Acids

54.23

Weight (kDa)

5.45

Isoelectric Point (pI)

31.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 33 - 487 3.1e-164 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018038)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g10810
malus_domestica MD06G1120700.v1.1
prunus_persica Prupe.5G131300_v2.0.a1
pyrus_communis pycom06g11200
rosa_chinensis RchiOBHm_Chr7g0187351
rosa_laevigata RLG00000004792
rosa_multiflora Rmu_sc0001758.1_g000011
rosa_roxburghii Rroxscaffold_3G00267570
rosa_rugosa Rorug06G0480800
rosa_samantha Rh7CG086300
rosa_wichuraiana Rw7G007500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 811, 1451
AccII CGCG 3 cut(s) 490, 743, 1391
AccIII TCCGGA 2 cut(s) 602, 713
AciI CCGC 5 cut(s) 234, 488, 1285, 1365, 1391
AclWI GGATC 3 cut(s) 431, 973, 1120
AcoI YGGCCR 1 cut(s) 802
AcsI RAATTY 1 cut(s) 911
AcuI CTGAAG 2 cut(s) 111, 927
AfaI GTAC 7 cut(s) 366, 653, 1010, 1079, 1239, 1269, 1313
AfiI CCNNNNNNNGG 3 cut(s) 369, 811, 1451
AflIII ACRYGT 1 cut(s) 1106
AgsI TTSAA 3 cut(s) 347, 580, 1089
AjnI CCWGG 6 cut(s) 409, 520, 746, 961, 994, 1279
AjuI GAANNNNNNNTTGG 2 cut(s) 714, 746
AluBI AGCT 8 cut(s) 66, 327, 456, 566, 623, 730, 888, 1121
AluI AGCT 8 cut(s) 66, 327, 456, 566, 623, 730, 888, 1121
Alw21I GWGCWC 1 cut(s) 377
Alw26I GTCTC 1 cut(s) 890
AlwI GGATC 3 cut(s) 431, 973, 1120
AlwNI CAGNNNCTG 2 cut(s) 459, 545
Aor13HI TCCGGA 2 cut(s) 602, 713
AoxI GGCC 7 cut(s) 136, 279, 467, 802, 981, 1282, 1353
ApoI RAATTY 1 cut(s) 911
AspLEI GCGC 2 cut(s) 492, 743
AspS9I GGNCC 7 cut(s) 136, 448, 525, 871, 982, 1353, 1367
AsuHPI GGTGA 1 cut(s) 1190
AvaII GGWCC 4 cut(s) 448, 525, 871, 1367
BalI TGGCCA 1 cut(s) 804
BanII GRGCYC 1 cut(s) 1459
BbsI GAAGAC 1 cut(s) 568
Bbv12I GWGCWC 1 cut(s) 377
BbvI GCAGC 9 cut(s) 59, 346, 407, 443, 535, 538, 547, 548, 575
BccI CCATC 4 cut(s) 166, 197, 1270, 1295
BceAI ACGGC 1 cut(s) 347
BciT130I CCWGG 6 cut(s) 411, 522, 748, 963, 996, 1281
BciVI GTATCC 3 cut(s) 475, 710, 1236
BcoDI GTCTC 1 cut(s) 890
BfaI CTAG 2 cut(s) 893, 972
BfmI CTRYAG 1 cut(s) 561
BfuI GTATCC 3 cut(s) 475, 710, 1236
BmcAI AGTACT 1 cut(s) 1313
Bme1390I CCNGG 6 cut(s) 411, 522, 748, 963, 996, 1281
Bme18I GGWCC 4 cut(s) 448, 525, 871, 1367
BmgT120I GGNCC 7 cut(s) 136, 448, 525, 871, 982, 1353, 1367
BmiI GGNNCC 5 cut(s) 208, 415, 526, 1327, 1354
BmrFI CCNGG 6 cut(s) 411, 522, 748, 963, 996, 1281
BmsI GCATC 2 cut(s) 94, 235
BpiI GAAGAC 1 cut(s) 568
BpmI CTGGAG 3 cut(s) 270, 1016, 1017
Bpu10I CCTNAGC 1 cut(s) 67
BsaI GGTCTC 1 cut(s) 890
BsaJI CCNNGG 7 cut(s) 354, 409, 521, 676, 994, 1174, 1279
BsaWI WCCGGW 3 cut(s) 602, 713, 1398
BsaXI ACNNNNNCTCC 6 cut(s) 243, 273, 656, 686, 1044, 1074
Bsc4I CCNNNNNNNGG 3 cut(s) 369, 811, 1451
Bse1I ACTGG 2 cut(s) 561, 1033
Bse3DI GCAATG 1 cut(s) 499
BseAI TCCGGA 2 cut(s) 602, 713
BseBI CCWGG 6 cut(s) 411, 522, 748, 963, 996, 1281
BseDI CCNNGG 7 cut(s) 354, 409, 521, 676, 994, 1174, 1279
BseGI GGATG 3 cut(s) 853, 1170, 1399
BseLI CCNNNNNNNGG 3 cut(s) 369, 811, 1451
BseMI GCAATG 1 cut(s) 499
BseMII CTCAG 3 cut(s) 114, 696, 948
BseNI ACTGG 2 cut(s) 561, 1033
BseRI GAGGAG 4 cut(s) 199, 1046, 1049, 1052
BseXI GCAGC 9 cut(s) 59, 346, 407, 443, 535, 538, 547, 548, 575
BseYI CCCAGC 1 cut(s) 459
BsgI GTGCAG 1 cut(s) 537
Bsh1236I CGCG 3 cut(s) 490, 743, 1391
BshFI GGCC 7 cut(s) 138, 281, 469, 804, 983, 1284, 1355
BsiHKAI GWGCWC 1 cut(s) 377
BsiSI CCGG 5 cut(s) 603, 714, 861, 1187, 1399
BsiWI CGTACG 1 cut(s) 364
BslI CCNNNNNNNGG 3 cut(s) 369, 811, 1451
BsmAI GTCTC 1 cut(s) 890
BsnI GGCC 7 cut(s) 138, 281, 469, 804, 983, 1284, 1355
Bso31I GGTCTC 1 cut(s) 890
Bsp1286I GDGCHC 2 cut(s) 377, 1459
Bsp13I TCCGGA 2 cut(s) 602, 713
Bsp1407I TGTACA 3 cut(s) 1008, 1237, 1267
Bsp143I GATC 3 cut(s) 436, 965, 1112
BspACI CCGC 5 cut(s) 234, 488, 1285, 1365, 1391
BspANI GGCC 7 cut(s) 138, 281, 469, 804, 983, 1284, 1355
BspCNI CTCAG 3 cut(s) 113, 695, 947
BspEI TCCGGA 2 cut(s) 602, 713
BspFNI CGCG 3 cut(s) 490, 743, 1391
BspLI GGNNCC 5 cut(s) 208, 415, 526, 1327, 1354
BspMAI CTGCAG 1 cut(s) 565
BspPI GGATC 3 cut(s) 431, 973, 1120
BspTNI GGTCTC 1 cut(s) 890
BsrDI GCAATG 1 cut(s) 499
BsrGI TGTACA 3 cut(s) 1008, 1237, 1267
BsrI ACTGG 2 cut(s) 561, 1033
BssECI CCNNGG 7 cut(s) 354, 409, 521, 676, 994, 1174, 1279
BssMI GATC 3 cut(s) 436, 965, 1112
BssT1I CCWWGG 2 cut(s) 354, 1174
Bst2UI CCWGG 6 cut(s) 411, 522, 748, 963, 996, 1281
Bst4CI ACNGT 3 cut(s) 369, 668, 773
Bst6I CTCTTC 1 cut(s) 131
BstAPI GCANNNNNTGC 3 cut(s) 545, 560, 1293
BstAUI TGTACA 3 cut(s) 1008, 1237, 1267
BstC8I GCNNGC 2 cut(s) 858, 1425
BstDEI CTNAG 7 cut(s) 25, 62, 67, 100, 682, 934, 1082
BstF5I GGATG 3 cut(s) 853, 1170, 1399
BstFNI CGCG 3 cut(s) 490, 743, 1391
BstHHI GCGC 2 cut(s) 492, 743
BstKTI GATC 3 cut(s) 439, 968, 1115
BstMAI GTCTC 1 cut(s) 890
BstMBI GATC 3 cut(s) 436, 965, 1112
BstNI CCWGG 6 cut(s) 411, 522, 748, 963, 996, 1281
BstNSI RCATGY 3 cut(s) 199, 1110, 1238
BstSCI CCNGG 6 cut(s) 409, 520, 746, 961, 994, 1279
BstSFI CTRYAG 1 cut(s) 561
BstUI CGCG 3 cut(s) 490, 743, 1391
BstV1I GCAGC 9 cut(s) 59, 346, 407, 443, 535, 538, 547, 548, 575
BstV2I GAAGAC 1 cut(s) 568
BstX2I RGATCY 1 cut(s) 436
BstXI CCANNNNNNTGG 1 cut(s) 146
BstYI RGATCY 1 cut(s) 436
BsuI GTATCC 3 cut(s) 475, 710, 1236
BsuRI GGCC 7 cut(s) 138, 281, 469, 804, 983, 1284, 1355
BtsCI GGATG 3 cut(s) 853, 1170, 1399
BtsIMutI CAGTG 4 cut(s) 554, 673, 690, 903
Cac8I GCNNGC 2 cut(s) 858, 1425
CaiI CAGNNNCTG 2 cut(s) 459, 545
CfoI GCGC 2 cut(s) 492, 743
Cfr13I GGNCC 7 cut(s) 136, 448, 525, 871, 982, 1353, 1367
CseI GACGC 1 cut(s) 732
Csp6I GTAC 7 cut(s) 365, 652, 1009, 1078, 1238, 1268, 1312
CviAII CATG 8 cut(s) 196, 218, 303, 445, 1107, 1204, 1235, 1271
CviQI GTAC 7 cut(s) 365, 652, 1009, 1078, 1238, 1268, 1312
DdeI CTNAG 7 cut(s) 25, 62, 67, 100, 682, 934, 1082
DpnI GATC 3 cut(s) 438, 967, 1114
DpnII GATC 3 cut(s) 436, 965, 1112
EaeI YGGCCR 1 cut(s) 802
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
EciI GGCGGA 1 cut(s) 1380
Eco130I CCWWGG 2 cut(s) 354, 1174
Eco24I GRGCYC 1 cut(s) 1459
Eco31I GGTCTC 1 cut(s) 890
Eco47I GGWCC 4 cut(s) 448, 525, 871, 1367
Eco57I CTGAAG 2 cut(s) 111, 927
EcoO109I RGGNCCY 1 cut(s) 871
EcoRI GAATTC 1 cut(s) 911
EcoRII CCWGG 6 cut(s) 409, 520, 746, 961, 994, 1279
EcoT14I CCWWGG 2 cut(s) 354, 1174
EcoT38I GRGCYC 1 cut(s) 1459
ErhI CCWWGG 2 cut(s) 354, 1174
FaeI CATG 8 cut(s) 199, 221, 306, 448, 1110, 1207, 1238, 1274
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FatI CATG 8 cut(s) 195, 217, 302, 444, 1106, 1203, 1234, 1270
FauNDI CATATG 2 cut(s) 947, 1069
FokI GGATG 3 cut(s) 860, 1157, 1406
FriOI GRGCYC 1 cut(s) 1459
FspBI CTAG 2 cut(s) 893, 972
GlaI GCGC 2 cut(s) 491, 742
GsaI CCCAGC 1 cut(s) 463
GsuI CTGGAG 3 cut(s) 270, 1016, 1017
HaeIII GGCC 7 cut(s) 138, 281, 469, 804, 983, 1284, 1355
HapII CCGG 5 cut(s) 603, 714, 861, 1187, 1399
HgaI GACGC 1 cut(s) 732
HhaI GCGC 2 cut(s) 492, 743
Hin1II CATG 8 cut(s) 199, 221, 306, 448, 1110, 1207, 1238, 1274
Hin6I GCGC 2 cut(s) 490, 741
HinP1I GCGC 2 cut(s) 490, 741
HincII GTYRAC 1 cut(s) 510
HindII GTYRAC 1 cut(s) 510
HinfI GANTC 4 cut(s) 49, 584, 671, 751
HpaII CCGG 5 cut(s) 603, 714, 861, 1187, 1399
HphI GGTGA 1 cut(s) 1190
Hpy166II GTNNAC 5 cut(s) 59, 499, 510, 1165, 1240
Hpy188I TCNGA 6 cut(s) 103, 202, 529, 589, 937, 1420
Hpy188III TCNNGA 5 cut(s) 110, 603, 714, 893, 916
Hpy8I GTNNAC 5 cut(s) 59, 499, 510, 1165, 1240
HpyAV CCTTC 3 cut(s) 91, 292, 603
HpyCH4III ACNGT 3 cut(s) 369, 668, 773
HpyCH4V TGCA 7 cut(s) 536, 554, 563, 644, 1073, 1296, 1442
HpyF3I CTNAG 7 cut(s) 25, 62, 67, 100, 682, 934, 1082
Hsp92II CATG 8 cut(s) 199, 221, 306, 448, 1110, 1207, 1238, 1274
HspAI GCGC 2 cut(s) 490, 741
Kpn2I TCCGGA 2 cut(s) 602, 713
Kzo9I GATC 3 cut(s) 436, 965, 1112
LmnI GCTCC 2 cut(s) 212, 372
Lsp1109I GCAGC 9 cut(s) 59, 346, 407, 443, 535, 538, 547, 548, 575
LweI GCATC 2 cut(s) 94, 235
MaeI CTAG 2 cut(s) 893, 972
MaeIII GTNAC 6 cut(s) 28, 668, 1039, 1102, 1123, 1178
MalI GATC 3 cut(s) 438, 967, 1114
MboI GATC 3 cut(s) 436, 965, 1112
MfeI CAATTG 1 cut(s) 175
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 2 cut(s) 377, 1459
MlsI TGGCCA 1 cut(s) 804
MluCI AATT 5 cut(s) 175, 187, 608, 911, 957
MluNI TGGCCA 1 cut(s) 804
MlyI GAGTC 3 cut(s) 43, 578, 665
Mox20I TGGCCA 1 cut(s) 804
MroI TCCGGA 2 cut(s) 602, 713
MscI TGGCCA 1 cut(s) 804
MseI TTAA 1 cut(s) 389
MslI CAYNNNNRTG 1 cut(s) 623
Msp20I TGGCCA 1 cut(s) 804
MspA1I CMGCKG 2 cut(s) 456, 1121
MspI CCGG 5 cut(s) 603, 714, 861, 1187, 1399
MspR9I CCNGG 6 cut(s) 411, 522, 748, 963, 996, 1281
MunI CAATTG 1 cut(s) 175
MvaI CCWGG 6 cut(s) 411, 522, 748, 963, 996, 1281
MvnI CGCG 3 cut(s) 490, 743, 1391
NdeI CATATG 2 cut(s) 947, 1069
NdeII GATC 3 cut(s) 436, 965, 1112
NlaIII CATG 8 cut(s) 199, 221, 306, 448, 1110, 1207, 1238, 1274
NlaIV GGNNCC 5 cut(s) 208, 415, 526, 1327, 1354
NmeAIII GCCGAG 1 cut(s) 657
NmuCI GTSAC 4 cut(s) 28, 668, 1123, 1178
NspI RCATGY 3 cut(s) 199, 1110, 1238
PciI ACATGT 1 cut(s) 1106
PfeI GAWTC 1 cut(s) 751
Pfl23II CGTACG 1 cut(s) 364
PflMI CCANNNNNTGG 2 cut(s) 811, 1451
PfoI TCCNGGA 2 cut(s) 746, 961
PleI GAGTC 3 cut(s) 43, 578, 665
PpsI GAGTC 3 cut(s) 43, 578, 665
PpuMI RGGWCCY 1 cut(s) 871
PscI ACATGT 1 cut(s) 1106
Psp5II RGGWCCY 1 cut(s) 871
Psp6I CCWGG 6 cut(s) 409, 520, 746, 961, 994, 1279
PspFI CCCAGC 1 cut(s) 459
PspGI CCWGG 6 cut(s) 409, 520, 746, 961, 994, 1279
PspLI CGTACG 1 cut(s) 364
PspN4I GGNNCC 5 cut(s) 208, 415, 526, 1327, 1354
PspPI GGNCC 7 cut(s) 136, 448, 525, 871, 982, 1353, 1367
PspPPI RGGWCCY 1 cut(s) 871
PsrI GAACNNNNNNTAC 2 cut(s) 761, 793
PstI CTGCAG 1 cut(s) 565
PstNI CAGNNNCTG 2 cut(s) 459, 545
PsuI RGATCY 1 cut(s) 436
PvuII CAGCTG 2 cut(s) 456, 1121
RsaI GTAC 7 cut(s) 366, 653, 1010, 1079, 1239, 1269, 1313
RsaNI GTAC 7 cut(s) 365, 652, 1009, 1078, 1238, 1268, 1312
RseI CAYNNNNRTG 1 cut(s) 623
SaqAI TTAA 1 cut(s) 389
Sau3AI GATC 3 cut(s) 436, 965, 1112
Sau96I GGNCC 7 cut(s) 136, 448, 525, 871, 982, 1353, 1367
ScaI AGTACT 1 cut(s) 1313
SchI GAGTC 3 cut(s) 43, 578, 665
ScrFI CCNGG 6 cut(s) 411, 522, 748, 963, 996, 1281
SduI GDGCHC 2 cut(s) 377, 1459
SfaNI GCATC 2 cut(s) 94, 235
SfcI CTRYAG 1 cut(s) 561
SinI GGWCC 4 cut(s) 448, 525, 871, 1367
SmiMI CAYNNNNRTG 1 cut(s) 623
Sse9I AATT 5 cut(s) 175, 187, 608, 911, 957
SsiI CCGC 5 cut(s) 234, 488, 1285, 1365, 1391
SspMI CTAG 2 cut(s) 893, 972
StyD4I CCNGG 6 cut(s) 409, 520, 746, 961, 994, 1279
StyI CCWWGG 2 cut(s) 354, 1174
TaaI ACNGT 3 cut(s) 369, 668, 773
TaqI TCGA 2 cut(s) 132, 764
TasI AATT 5 cut(s) 175, 187, 608, 911, 957
TatI WGTACW 5 cut(s) 651, 1008, 1237, 1267, 1311
TauI GCSGC 2 cut(s) 237, 1287
TfiI GAWTC 1 cut(s) 751
Tru1I TTAA 1 cut(s) 389
Tru9I TTAA 1 cut(s) 389
TscAI CASTG 4 cut(s) 561, 673, 690, 910
TseFI GTSAC 4 cut(s) 28, 668, 1123, 1178
Tsp45I GTSAC 4 cut(s) 28, 668, 1123, 1178
TspDTI ATGAA 3 cut(s) 743, 765, 1287
TspRI CASTG 4 cut(s) 561, 673, 690, 910
Van91I CCANNNNNTGG 2 cut(s) 811, 1451
VpaK11BI GGWCC 4 cut(s) 448, 525, 871, 1367
XapI RAATTY 1 cut(s) 911
XbaI TCTAGA 1 cut(s) 892
XceI RCATGY 3 cut(s) 199, 1110, 1238
XcmI CCANNNNNNNNNTGG 1 cut(s) 992
XspI CTAG 2 cut(s) 893, 972
ZrmI AGTACT 1 cut(s) 1313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.