pycom06g11200

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0018038
Physical Location & Seq
Forward (+)
16512201 .. 16514632
2432 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g11200.5

Sequence Viewer

Length: 1488 bp
ATGGCTTCTGCTGTCACATTTTCTTTAGGGGCAAAAATTCTATGCTTAACTCTTTGTCTGCTGAGCTTATGCTGCTCTGCTTTCACTCCTCTAGACTACTCAAATGCTCTCGAAAAGTCGATTCTCTTCTTCGAGGGTCAGAGGTCCGGGAAACTGCCCGACAACCAGCGGCTCAGTTGGAGGGGGAATTCCGGCTTGTCCGATGGCTCTTCCTACCATGTGGACTTAGTAGGAGGCTACTATGATGCTGGAGACAATGTCAAGTTTGGCCTGCCAATGGCCTTCACCACTACATTGCTGGCATGGAGTGTGATTGAATTTGGTGACTCTATGCATAGCCAGATTGACAATGCGAAAGCCGCCGTACGTTGGAGCACTGATTATCTTCTAAAAGCAGCCACTGCAACCCCCGGAACGTTATATGTCCAAGTGGCAGATCCCAACATGGACCACCGGTGCTGGGAGAGGCCAGAAGACATGGATACGCCACGCAATGTGTACAAGGTGTCAACCCAAAATCCAGGATCAGATGTTGCAGCTGAGACTGCTGCTGCATTGGCTGCCGCTTCCATAGTATTCAAAGACTCCGACTCTTCTTACTCTGCAAAATTGCTTCACACAGCAATGAAAGTGTTTGATTTTGCAGACAAGTACAGAGGTTCTTACAGTGACTCACTTGGTTCAGTGGTCTGCCCTTTTTACTGCTCATACTCAGGATACCATGATGAGCTTCTGTGGGGTGCATCATGGATCCATAGAGCCTCTCAGAACAGTTCATATCTTGCCTACATCAAGTCCAATGGCCACACATTGGGTGCAGAAGACGATGACTATTCCTTCAGTTGGGATGACAAGAGACCTGGAACTAAAGTTCTCCTTTCCAAGAGCTTTCTAGAGAAAAATGATGAGGAATTCCAGTTATACAAATTACATTCAGACAACTACATATGCTCTCTAATTCCAGGGACATCTACTTTTCAGGGCCAAAATACCCCTGGAGGATTGATCTACAAGGCAAGTGAGAGCAATCTCCAATATGTCACATCAGCATCTTTACTCCTCCTAACATATGCAAAGTATCTACGTTTAAATGGAGGAGTGGCAACATGTGGATCATCAAAAGTCACAGCAGAAACCCTAATCTCAGAGGCAAAGAAGCAGGTGGACTACATTCTCGGTGACAATCCAGCAAAGATGTCATACATGGTAGGGTTTGGGGACAAGTTCCCGCAGCATGTGCACCACAGGGGTTCTTCTCTCCCATCAGTCCATCAACATCCCAATCGCCTTGGCTGCAACGATGGATTTCAGTACCTGAATTCTGGTTCACCTAATCCAAATGTTCTTGTTGGAGCTGTTGTTGGTGGACCTGACAGTAAGGATAATTTTGCCAACGATCGGAACAATTATCAGCAGTCTGAGCCGGCTACTTACATCAATGCACCATTTGTTGGGGCTCTTGCTTTCCTCTCAGCTCAAAAGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

496

Amino Acids

53.98

Weight (kDa)

5.8

Isoelectric Point (pI)

30.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018038)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g10810
malus_domestica MD06G1120700.v1.1
prunus_persica Prupe.5G131300_v2.0.a1
pyrus_communis pycom06g11200
rosa_chinensis RchiOBHm_Chr7g0187351
rosa_laevigata RLG00000004792
rosa_multiflora Rmu_sc0001758.1_g000011
rosa_roxburghii Rroxscaffold_3G00267570
rosa_rugosa Rorug06G0480800
rosa_samantha Rh7CG086300
rosa_wichuraiana Rw7G007500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1150
Acc36I ACCTGC 1 cut(s) 1150
AccB7I CCANNNNNTGG 2 cut(s) 811, 1451
AciI CCGC 4 cut(s) 169, 360, 564, 1229
AclI AACGTT 1 cut(s) 416
AclWI GGATC 5 cut(s) 431, 532, 745, 758, 1120
AcoI YGGCCR 1 cut(s) 802
AcsI RAATTY 5 cut(s) 36, 187, 317, 911, 1318
AcuI CTGAAG 1 cut(s) 823
AfaI GTAC 4 cut(s) 366, 500, 653, 1313
AfiI CCNNNNNNNGG 7 cut(s) 277, 369, 460, 811, 843, 1398, 1451
AflIII ACRYGT 1 cut(s) 1106
AgeI ACCGGT 1 cut(s) 453
AgsI TTSAA 2 cut(s) 317, 580
AjnI CCWGG 4 cut(s) 520, 859, 961, 994
AloI GAACNNNNNNTCC 2 cut(s) 855, 887
AluBI AGCT 6 cut(s) 66, 539, 730, 888, 1355, 1475
AluI AGCT 6 cut(s) 66, 539, 730, 888, 1355, 1475
Alw21I GWGCWC 2 cut(s) 377, 1242
Alw26I GTCTC 3 cut(s) 246, 536, 850
Alw44I GTGCAC 1 cut(s) 1238
AlwI GGATC 5 cut(s) 431, 532, 745, 758, 1120
AlwNI CAGNNNCTG 2 cut(s) 401, 1315
AoxI GGCC 5 cut(s) 268, 279, 467, 802, 982
ApaLI GTGCAC 1 cut(s) 1238
ApeKI GCWGC 8 cut(s) 72, 395, 536, 548, 551, 560, 1231, 1293
ApoI RAATTY 5 cut(s) 36, 187, 317, 911, 1318
AsiGI ACCGGT 1 cut(s) 453
AspS9I GGNCC 4 cut(s) 144, 448, 982, 1367
AsuC2I CCSGG 2 cut(s) 148, 411
AsuHPI GGTGA 4 cut(s) 277, 335, 1190, 1320
AvaII GGWCC 3 cut(s) 144, 448, 1367
BaeGI GKGCMC 1 cut(s) 1242
BalI TGGCCA 1 cut(s) 804
BamHI GGATCC 1 cut(s) 750
BanII GRGCYC 1 cut(s) 1459
BbsI GAAGAC 2 cut(s) 480, 828
Bbv12I GWGCWC 2 cut(s) 377, 1242
BbvI GCAGC 8 cut(s) 59, 407, 535, 538, 547, 548, 1243, 1280
BccI CCATC 4 cut(s) 197, 1270, 1278, 1295
BceAI ACGGC 1 cut(s) 347
BciT130I CCWGG 4 cut(s) 522, 861, 963, 996
BciVI GTATCC 2 cut(s) 475, 710
BcnI CCSGG 2 cut(s) 148, 411
BcoDI GTCTC 3 cut(s) 246, 536, 850
BfaI CTAG 2 cut(s) 92, 893
BfuAI ACCTGC 1 cut(s) 1150
BfuI GTATCC 2 cut(s) 475, 710
BlpI GCTNAGC 1 cut(s) 62
Bme1390I CCNGG 6 cut(s) 148, 411, 522, 861, 963, 996
Bme18I GGWCC 3 cut(s) 144, 448, 1367
BmgT120I GGNCC 4 cut(s) 144, 448, 982, 1367
BmiI GGNNCC 1 cut(s) 752
BmrFI CCNGG 6 cut(s) 148, 411, 522, 861, 963, 996
BmsI GCATC 3 cut(s) 235, 752, 1058
BpiI GAAGAC 2 cut(s) 480, 828
BpmI CTGGAG 2 cut(s) 270, 1017
Bpu1102I GCTNAGC 1 cut(s) 62
BpuMI CCSGG 2 cut(s) 148, 411
BsaI GGTCTC 1 cut(s) 850
BsaJI CCNNGG 4 cut(s) 409, 962, 994, 1288
BsaWI WCCGGW 1 cut(s) 453
BsaXI ACNNNNNCTCC 4 cut(s) 243, 273, 1344, 1374
Bsc4I CCNNNNNNNGG 7 cut(s) 277, 369, 460, 811, 843, 1398, 1451
Bse118I RCCGGY 2 cut(s) 453, 1423
Bse1I ACTGG 1 cut(s) 916
Bse3DI GCAATG 3 cut(s) 293, 499, 630
BseBI CCWGG 4 cut(s) 522, 861, 963, 996
BseDI CCNNGG 4 cut(s) 409, 962, 994, 1288
BseGI GGATG 2 cut(s) 853, 1276
BseLI CCNNNNNNNGG 7 cut(s) 277, 369, 460, 811, 843, 1398, 1451
BseMI GCAATG 3 cut(s) 293, 499, 630
BseMII CTCAG 8 cut(s) 53, 187, 531, 726, 779, 1158, 1410, 1485
BseNI ACTGG 1 cut(s) 916
BseRI GAGGAG 3 cut(s) 78, 1049, 1110
BseSI GKGCMC 1 cut(s) 1242
BseXI GCAGC 8 cut(s) 59, 407, 535, 538, 547, 548, 1243, 1280
BseYI CCCAGC 1 cut(s) 459
BsgI GTGCAG 1 cut(s) 837
Bsh1285I CGRYCG 1 cut(s) 1399
BshFI GGCC 5 cut(s) 270, 281, 469, 804, 984
BshTI ACCGGT 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 1399
BsiHKAI GWGCWC 2 cut(s) 377, 1242
BsiSI CCGG 5 cut(s) 147, 192, 411, 454, 1424
BsiWI CGTACG 1 cut(s) 364
BslFI GGGAC 2 cut(s) 979, 1232
BslI CCNNNNNNNGG 7 cut(s) 277, 369, 460, 811, 843, 1398, 1451
BsmAI GTCTC 3 cut(s) 246, 536, 850
BsmFI GGGAC 2 cut(s) 979, 1232
BsnI GGCC 5 cut(s) 270, 281, 469, 804, 984
Bso31I GGTCTC 1 cut(s) 850
Bsp1286I GDGCHC 3 cut(s) 377, 1242, 1459
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 6 cut(s) 436, 524, 750, 1005, 1112, 1396
Bsp1720I GCTNAGC 1 cut(s) 62
BspACI CCGC 4 cut(s) 169, 360, 564, 1229
BspANI GGCC 5 cut(s) 270, 281, 469, 804, 984
BspCNI CTCAG 8 cut(s) 54, 186, 532, 725, 778, 1157, 1411, 1484
BspLI GGNNCC 1 cut(s) 752
BspMI ACCTGC 1 cut(s) 1150
BspPI GGATC 5 cut(s) 431, 532, 745, 758, 1120
BspQI GCTCTTC 1 cut(s) 214
BspTNI GGTCTC 1 cut(s) 850
BsrDI GCAATG 3 cut(s) 293, 499, 630
BsrFI RCCGGY 2 cut(s) 453, 1423
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 1 cut(s) 916
BssAI RCCGGY 2 cut(s) 453, 1423
BssECI CCNNGG 4 cut(s) 409, 962, 994, 1288
BssMI GATC 6 cut(s) 436, 524, 750, 1005, 1112, 1396
BssT1I CCWWGG 1 cut(s) 1288
Bst2UI CCWGG 4 cut(s) 522, 861, 963, 996
Bst4CI ACNGT 3 cut(s) 668, 773, 1376
Bst6I CTCTTC 3 cut(s) 131, 214, 598
BstAPI GCANNNNNTGC 3 cut(s) 401, 560, 1237
BstAUI TGTACA 1 cut(s) 498
BstC8I GCNNGC 3 cut(s) 272, 300, 1425
BstDEI CTNAG 9 cut(s) 62, 173, 226, 540, 712, 765, 1144, 1419, 1471
BstF5I GGATG 2 cut(s) 853, 1276
BstKTI GATC 6 cut(s) 439, 527, 753, 1008, 1115, 1399
BstMAI GTCTC 3 cut(s) 246, 536, 850
BstMBI GATC 6 cut(s) 436, 524, 750, 1005, 1112, 1396
BstMCI CGRYCG 1 cut(s) 1399
BstMWI GCNNNNNNNGC 9 cut(s) 72, 359, 401, 545, 557, 560, 1237, 1293, 1420
BstNI CCWGG 4 cut(s) 522, 861, 963, 996
BstNSI RCATGY 2 cut(s) 1110, 1238
BstSCI CCNGG 6 cut(s) 146, 409, 520, 859, 961, 994
BstSLI GKGCMC 1 cut(s) 1242
BstV1I GCAGC 8 cut(s) 59, 407, 535, 538, 547, 548, 1243, 1280
BstV2I GAAGAC 2 cut(s) 480, 828
BstX2I RGATCY 2 cut(s) 436, 750
BstYI RGATCY 2 cut(s) 436, 750
BsuI GTATCC 2 cut(s) 475, 710
BsuRI GGCC 5 cut(s) 270, 281, 469, 804, 984
BtsCI GGATG 2 cut(s) 853, 1276
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 4 cut(s) 375, 399, 673, 690
BveI ACCTGC 1 cut(s) 1150
Cac8I GCNNGC 3 cut(s) 272, 300, 1425
CaiI CAGNNNCTG 2 cut(s) 401, 1315
Cfr10I RCCGGY 2 cut(s) 453, 1423
Cfr13I GGNCC 4 cut(s) 144, 448, 982, 1367
Csp6I GTAC 4 cut(s) 365, 499, 652, 1312
CspAI ACCGGT 1 cut(s) 453
CviAII CATG 9 cut(s) 218, 303, 445, 478, 722, 747, 1107, 1204, 1235
CviQI GTAC 4 cut(s) 365, 499, 652, 1312
DdeI CTNAG 9 cut(s) 62, 173, 226, 540, 712, 765, 1144, 1419, 1471
DpnI GATC 6 cut(s) 438, 526, 752, 1007, 1114, 1398
DpnII GATC 6 cut(s) 436, 524, 750, 1005, 1112, 1396
DraI TTTAAA 1 cut(s) 1089
EaeI YGGCCR 1 cut(s) 802
Eam1104I CTCTTC 3 cut(s) 131, 214, 598
EarI CTCTTC 3 cut(s) 131, 214, 598
Eco130I CCWWGG 1 cut(s) 1288
Eco24I GRGCYC 1 cut(s) 1459
Eco31I GGTCTC 1 cut(s) 850
Eco47I GGWCC 3 cut(s) 144, 448, 1367
Eco57I CTGAAG 1 cut(s) 823
EcoRI GAATTC 3 cut(s) 187, 911, 1318
EcoRII CCWGG 4 cut(s) 520, 859, 961, 994
EcoT14I CCWWGG 1 cut(s) 1288
EcoT22I ATGCAT 1 cut(s) 336
EcoT38I GRGCYC 1 cut(s) 1459
ErhI CCWWGG 1 cut(s) 1288
FaeI CATG 9 cut(s) 221, 306, 448, 481, 725, 750, 1110, 1207, 1238
FalI AAGNNNNNCTT 2 cut(s) 861, 893
FaqI GGGAC 2 cut(s) 979, 1232
FatI CATG 9 cut(s) 217, 302, 444, 477, 721, 746, 1106, 1203, 1234
FauI CCCGC 1 cut(s) 1236
FauNDI CATATG 2 cut(s) 947, 1069
FokI GGATG 2 cut(s) 860, 1263
FriOI GRGCYC 1 cut(s) 1459
FspBI CTAG 2 cut(s) 92, 893
GsaI CCCAGC 1 cut(s) 463
GsuI CTGGAG 2 cut(s) 270, 1017
HaeIII GGCC 5 cut(s) 270, 281, 469, 804, 984
HapII CCGG 5 cut(s) 147, 192, 411, 454, 1424
Hin1II CATG 9 cut(s) 221, 306, 448, 481, 725, 750, 1110, 1207, 1238
HincII GTYRAC 1 cut(s) 510
HindII GTYRAC 1 cut(s) 510
HinfI GANTC 5 cut(s) 121, 326, 584, 590, 671
HpaII CCGG 5 cut(s) 147, 192, 411, 454, 1424
HphI GGTGA 4 cut(s) 277, 335, 1190, 1320
Hpy166II GTNNAC 7 cut(s) 223, 499, 510, 1165, 1240, 1328, 1367
Hpy188I TCNGA 9 cut(s) 141, 202, 529, 589, 768, 937, 1147, 1401, 1420
Hpy188III TCNNGA 4 cut(s) 92, 110, 714, 893
Hpy8I GTNNAC 7 cut(s) 223, 499, 510, 1165, 1240, 1328, 1367
HpyAV CCTTC 2 cut(s) 292, 847
HpyCH4III ACNGT 3 cut(s) 668, 773, 1376
HpyCH4IV ACGT 3 cut(s) 367, 416, 1084
HpyF10VI GCNNNNNNNGC 9 cut(s) 72, 359, 401, 545, 557, 560, 1237, 1293, 1420
HpyF3I CTNAG 9 cut(s) 62, 173, 226, 540, 712, 765, 1144, 1419, 1471
HpySE526I ACGT 3 cut(s) 367, 416, 1084
Hsp92II CATG 9 cut(s) 221, 306, 448, 481, 725, 750, 1110, 1207, 1238
KroI GCCGGC 1 cut(s) 1423
KroNI GCCGGC 1 cut(s) 1425
Kzo9I GATC 6 cut(s) 436, 524, 750, 1005, 1112, 1396
LguI GCTCTTC 1 cut(s) 214
LmnI GCTCC 2 cut(s) 372, 1352
Lsp1109I GCAGC 8 cut(s) 59, 407, 535, 538, 547, 548, 1243, 1280
LweI GCATC 3 cut(s) 235, 752, 1058
MaeI CTAG 2 cut(s) 92, 893
MaeII ACGT 3 cut(s) 367, 416, 1084
MaeIII GTNAC 6 cut(s) 13, 323, 668, 1039, 1123, 1178
MalI GATC 6 cut(s) 438, 526, 752, 1007, 1114, 1398
MboI GATC 6 cut(s) 436, 524, 750, 1005, 1112, 1396
MboII GAAGA 8 cut(s) 118, 121, 201, 377, 485, 585, 833, 1245
MflI RGATCY 2 cut(s) 436, 750
MhlI GDGCHC 3 cut(s) 377, 1242, 1459
MlsI TGGCCA 1 cut(s) 804
MluNI TGGCCA 1 cut(s) 804
MlyI GAGTC 4 cut(s) 320, 578, 584, 665
MmeI TCCRAC 4 cut(s) 158, 350, 612, 1330
Mox20I TGGCCA 1 cut(s) 804
Mph1103I ATGCAT 1 cut(s) 336
MroNI GCCGGC 1 cut(s) 1423
MscI TGGCCA 1 cut(s) 804
MseI TTAA 2 cut(s) 47, 1088
MslI CAYNNNNRTG 1 cut(s) 623
Msp20I TGGCCA 1 cut(s) 804
MspA1I CMGCKG 2 cut(s) 169, 539
MspI CCGG 5 cut(s) 147, 192, 411, 454, 1424
MspR9I CCNGG 6 cut(s) 148, 411, 522, 861, 963, 996
MvaI CCWGG 4 cut(s) 522, 861, 963, 996
MwoI GCNNNNNNNGC 9 cut(s) 72, 359, 401, 545, 557, 560, 1237, 1293, 1420
NaeI GCCGGC 1 cut(s) 1425
NciI CCSGG 2 cut(s) 148, 411
NdeI CATATG 2 cut(s) 947, 1069
NdeII GATC 6 cut(s) 436, 524, 750, 1005, 1112, 1396
NgoMIV GCCGGC 1 cut(s) 1423
NlaIII CATG 9 cut(s) 221, 306, 448, 481, 725, 750, 1110, 1207, 1238
NlaIV GGNNCC 1 cut(s) 752
NmuCI GTSAC 6 cut(s) 13, 323, 668, 1039, 1123, 1178
NsiI ATGCAT 1 cut(s) 336
NspI RCATGY 2 cut(s) 1110, 1238
PaqCI CACCTGC 1 cut(s) 1150
PciI ACATGT 1 cut(s) 1106
PciSI GCTCTTC 1 cut(s) 214
PdiI GCCGGC 1 cut(s) 1425
PfeI GAWTC 1 cut(s) 121
Pfl23II CGTACG 1 cut(s) 364
PflFI GACNNNGTC 1 cut(s) 257
PflMI CCANNNNNTGG 2 cut(s) 811, 1451
PfoI TCCNGGA 2 cut(s) 146, 520
PinAI ACCGGT 1 cut(s) 453
Ple19I CGATCG 1 cut(s) 1399
PleI GAGTC 4 cut(s) 320, 578, 584, 665
PpsI GAGTC 4 cut(s) 320, 578, 584, 665
PscI ACATGT 1 cut(s) 1106
Psp1406I AACGTT 1 cut(s) 416
Psp6I CCWGG 4 cut(s) 520, 859, 961, 994
PspFI CCCAGC 1 cut(s) 459
PspGI CCWGG 4 cut(s) 520, 859, 961, 994
PspLI CGTACG 1 cut(s) 364
PspN4I GGNNCC 1 cut(s) 752
PspPI GGNCC 4 cut(s) 144, 448, 982, 1367
PstNI CAGNNNCTG 2 cut(s) 401, 1315
PsuI RGATCY 2 cut(s) 436, 750
PsyI GACNNNGTC 1 cut(s) 257
PvuI CGATCG 1 cut(s) 1399
PvuII CAGCTG 1 cut(s) 539
RsaI GTAC 4 cut(s) 366, 500, 653, 1313
RsaNI GTAC 4 cut(s) 365, 499, 652, 1312
RseI CAYNNNNRTG 1 cut(s) 623
SapI GCTCTTC 1 cut(s) 214
SaqAI TTAA 2 cut(s) 47, 1088
Sau3AI GATC 6 cut(s) 436, 524, 750, 1005, 1112, 1396
Sau96I GGNCC 4 cut(s) 144, 448, 982, 1367
SchI GAGTC 4 cut(s) 320, 578, 584, 665
ScrFI CCNGG 6 cut(s) 148, 411, 522, 861, 963, 996
SduI GDGCHC 3 cut(s) 377, 1242, 1459
SfaNI GCATC 3 cut(s) 235, 752, 1058
SgrAI CRCCGGYG 1 cut(s) 453
SinI GGWCC 3 cut(s) 144, 448, 1367
SmiMI CAYNNNNRTG 1 cut(s) 623
SsiI CCGC 4 cut(s) 169, 360, 564, 1229
SspMI CTAG 2 cut(s) 92, 893
StyD4I CCNGG 6 cut(s) 146, 409, 520, 859, 961, 994
StyI CCWWGG 1 cut(s) 1288
TaaI ACNGT 3 cut(s) 668, 773, 1376
TaiI ACGT 3 cut(s) 370, 419, 1087
TaqI TCGA 3 cut(s) 111, 119, 132
TatI WGTACW 2 cut(s) 498, 651
TauI GCSGC 3 cut(s) 172, 362, 566
TfiI GAWTC 1 cut(s) 121
Tru1I TTAA 2 cut(s) 47, 1088
Tru9I TTAA 2 cut(s) 47, 1088
TscAI CASTG 4 cut(s) 382, 406, 673, 690
TseFI GTSAC 6 cut(s) 13, 323, 668, 1039, 1123, 1178
TseI GCWGC 8 cut(s) 72, 395, 536, 548, 551, 560, 1231, 1293
Tsp45I GTSAC 6 cut(s) 13, 323, 668, 1039, 1123, 1178
TspDTI ATGAA 2 cut(s) 641, 765
TspRI CASTG 4 cut(s) 382, 406, 673, 690
Tth111I GACNNNGTC 1 cut(s) 257
Van91I CCANNNNNTGG 2 cut(s) 811, 1451
VneI GTGCAC 1 cut(s) 1238
VpaK11BI GGWCC 3 cut(s) 144, 448, 1367
XapI RAATTY 5 cut(s) 36, 187, 317, 911, 1318
XbaI TCTAGA 2 cut(s) 91, 892
XceI RCATGY 2 cut(s) 1110, 1238
XcmI CCANNNNNNNNNTGG 2 cut(s) 295, 992
XspI CTAG 2 cut(s) 92, 893
Zsp2I ATGCAT 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.