Rorug06G0480800

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0018038
Physical Location & Seq
Forward (+)
60231028 .. 60234045
3018 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0480800.1

Sequence Viewer

Length: 2655 bp
ATGGCATCACAATGGTATAGGGGCAGAGTCAAGGCTGTTCCTTCAGGTGATTCCCTTGTCATCCAGGCCCTTCAGTCAAACAAGACCAGAATCCCACCGGAAAAAAGTCAAAACACTATTACTTTATTATCAGTTCGTGCTCCCAAACTGGCCGGTGAAGATCGCAGTACTGATGAGCCTTTTGCATGGGACAGCAGAGAGTATCTGAGGAAGCTCTGCATCGGAAAGGAGGTGGTTTTCAGCATTGAGCAGGGCGTCGGCTCTGTTTTCCTTGACAACAATAATGTTGCAGCCCTTGTTGTTCAAAAAGGGTGGGCAGCTGTCACGTCACCGAGCACAACTCCTTACAATTGTGAGCTGCAGTTTCACATGGATCAAGCCTTTAATAAAGGTCTTGGTCGCTGGAGCACGGTTCCAGGTGCCGCGGAGGCATCCATTAGGACTCTACCTCCCCAACTCAACTTAGAGACCATGAAGACTCTGTTGGCTGCAAACAAGGGCAAGCGCATAGAAGCTATTGTCGAGGAGGTTGTTGATGGCAGTATTGTTCTGGCTCACTTGCTTCCGGAGTTTCAGTTTGTCCCAGTCATTGTTGCAGGAACTCAGGCGCCCTACATTGGACCTGATGCTTATCCTTTGATTCCTTTCGCACTAGAAGCTAAGTTTTTCACGGAGTCTCGTGTGTTGAATAGAGATGTGCATATTGTTCTGGAAGGTGTTGATGAGTTCAGCAAGTTCAGCCGTCTGATTGGGTCTCTGTATTACCCGGATGGAGACTCCGAAAAGGACTTAGGGCTGGAGCTTGTGGAGAATGGTTATGCTAAATATGTACCATGGAGTGCGAAAGTGATGGGTGAAGATGCAAGGCAGCGGCTCAAGACTGCAGAGTTTGAAGCTAAGAAAAACAAGCTGAGGATTTGGACACACTTTGTACCCCCAGCTACAAATTCCAAGCCAATTCATGACCAGAATTTCACGGGAACAGTTGTGGAGGTTGTAAGTGGGGAATGTGTCATTGTGGCTAATGATTCTGGAGAGCGGAGAGTCAATCTTTCGAGTATTTGGTGTCCGAAGATGGGAAATCCTCGTAGAGGTGATAAGCCAGCTGCATATGCACGTGAAGCCAAGGAGTTGCTGAGGACACGCCTTATTGGACGCCAAGTGAATGTTCAAATGGAGTATTCAAGGAAGGTAGGCCAAGCAGATGCAGATTCTTCCAGAGTAATAGATTTTGGATCAGTCTTTATTAAGGCTGAGGGGGAAGATAAAAGCAACCAGGCTGAGGAAAACGTTGCTGAGCTTGTGGTTGCATGTGGCTTCGGCAATGTTATCAAACACCGAGATTTTGAGGAGAGATCAAGATACTATGATGCCCTTCTTGCTGCTGAATCAGGCGCTATTGCTGGAAAGAAAGGACTACATTCTTCCAAGGAGGCCCCCGTTGTGCACATGACAGACTTGACAATGATAACGGCCAAGAAAGCAAGAGACGTCTTGCCATCCTTGCAGCGAAGTAGGAGAGTTTCTGTAGTTGTGGAATATGTCCTCAGCGGTCATCGTTTTAAACTATACATTCCCAAGGAGACATGCAGCATTTCCTTCTCTCTCTCCGGTGTGAGATGTCCCGGTCGCGATGAGCCCTATTCGGATGAAGCAATTGCAGTAATGAGGCGAAGATTAATGCAGAGAGATGTTGAGATTGAAGTGGAGACTGTTGATAGAAACGGAACTTTCTTGGGAACTCTATGGGAGTCAGGGACCAATGCGTCAATTGCCCTCGTTGAAGCTGGCCTTGCCAAATATATTCCCTTTGGTGGCAGTGACAAGATCCAAGATGGCCACCTTCTTGAACGAGCTATGAAAAATGCAAAAACGAAGAAGCTGAAAATTTGGGAAAATTTTGAAGGAAAACCCAACAAACAGAAAGAACTAAAGGTAGTGATTACAGAAGTTTTGGACAGAGGTAAATTTTATGTGCAGACAGTTGGGGACGAGAAGAGTGCCTCTATTCAGCAACAGCTTACTTCTTTGAACCTTGAAGAAGCTCCTTCTGGAAGTGACGCTTTTAATCCTAGGGAGGGTGACATTGTCCTTGCTCAGTTTAGTGCAGATAATTCTTGGAACCGAGCTAGGATTGTCAATGCGCGAAAAGACAAGTTTGAAGTGTTCTACATTGACTATGGTAATCAAGAGGTTGTCCCTTACAGTCAGTTACGGCCTCTTAACTCTTCTGCTGCCTCTGCACCTGGTCTTGCTCAGCTATGCAGCCTTGCACATGTTACTGTCCCGAGCTTGGAGGAGGAGTTTGGTCAAGAAGCAGGAGAGTATTTGAGTGAGTGCACATTGGACAAAGAGTTCACGGCCGTGATTGAGGGAAGGGATATTTCCGGAGGAAAATTTGCTAAAGGCCAGGGAACCGGACCAGCTCTTATGGTAACTCTGTTCGCGGTAGATAATGCTGACAGTATAAATGCTGCCATGCTTCAGAATGGACTTGCTAGTCTAGATAACCAGAAGAGGTCTTGGAAGTCTCGTACCTTGAGAGAGTTCCAGGAAAAAGCACAAACCTTCCGCCAGGGGATGTGGCATGATTATTACGAGTCTGATCATCATGAGAACAGAGCTCCTCCTGCAAGAAAGGCTGCTGGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

884

Amino Acids

98.03

Weight (kDa)

6.75

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNase PF00565 211 - 308 1.8e-11 Staphylococcal nuclease homologue
SNase PF00565 534 - 633 1e-13 Staphylococcal nuclease homologue
TUDOR PF00567 640 - 759 1.5e-23 Tudor domain
SNase PF00565 765 - 864 1.3e-07 Staphylococcal nuclease homologue
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018038)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g10810
malus_domestica MD06G1120700.v1.1
prunus_persica Prupe.5G131300_v2.0.a1
pyrus_communis pycom06g11200
rosa_chinensis RchiOBHm_Chr7g0187351
rosa_laevigata RLG00000004792
rosa_multiflora Rmu_sc0001758.1_g000011
rosa_roxburghii Rroxscaffold_3G00267570
rosa_rugosa Rorug06G0480800
rosa_samantha Rh7CG086300
rosa_wichuraiana Rw7G007500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1765, 2499
AatII GACGTC 1 cut(s) 1494
AccB1I GGYRCC 2 cut(s) 419, 607
AccBSI CCGCTC 1 cut(s) 1039
AccII CGCG 4 cut(s) 425, 1632, 2146, 2447
AccIII TCCGGA 2 cut(s) 565, 2387
AciI CCGC 7 cut(s) 423, 425, 871, 1039, 1551, 2447, 2572
AclI AACGTT 1 cut(s) 1290
AclWI GGATC 3 cut(s) 381, 1243, 1822
AcoI YGGCCR 4 cut(s) 150, 1473, 1837, 2361
AcsI RAATTY 6 cut(s) 946, 970, 1887, 1897, 1967, 2396
AcuI CTGAAG 3 cut(s) 27, 56, 2468
AcvI CACGTG 1 cut(s) 1118
AcyI GRCGYC 4 cut(s) 255, 608, 1156, 1491
AfaI GTAC 4 cut(s) 169, 831, 933, 2536
AfiI CCNNNNNNNGG 7 cut(s) 617, 1076, 1091, 1282, 1814, 2078, 2293
AflIII ACRYGT 1 cut(s) 2275
AjiI CACGTC 1 cut(s) 327
AjnI CCWGG 7 cut(s) 63, 415, 1275, 2245, 2409, 2550, 2574
AjuI GAANNNNNNNTTGG 4 cut(s) 467, 499, 1152, 1184
AleI CACNNNNGTG 1 cut(s) 2363
AloI GAACNNNNNNTCC 2 cut(s) 2339, 2371
Alw21I GWGCWC 6 cut(s) 142, 338, 410, 1449, 2342, 2626
Alw26I GTCTC 8 cut(s) 461, 681, 759, 768, 1482, 1577, 1703, 2535
Alw44I GTGCAC 2 cut(s) 1445, 2338
AlwI GGATC 3 cut(s) 381, 1243, 1822
Ama87I CYCGRG 1 cut(s) 2287
Aor13HI TCCGGA 2 cut(s) 565, 2387
ApaLI GTGCAC 2 cut(s) 1445, 2338
ApoI RAATTY 6 cut(s) 946, 970, 1887, 1897, 1967, 2396
AseI ATTAAT 1 cut(s) 1679
AspA2I CCTAGG 1 cut(s) 2072
AspLEI GCGC 4 cut(s) 507, 610, 1397, 2146
AspS9I GGNCC 5 cut(s) 67, 620, 1435, 1758, 2420
AsuC2I CCSGG 2 cut(s) 767, 1626
AsuHPI GGTGA 6 cut(s) 59, 167, 321, 866, 1106, 2093
AvaI CYCGRG 1 cut(s) 2287
AvaII GGWCC 3 cut(s) 620, 1758, 2420
AvrII CCTAGG 1 cut(s) 2072
BaeGI GKGCMC 2 cut(s) 1449, 2342
BalI TGGCCA 1 cut(s) 1839
BanI GGYRCC 2 cut(s) 419, 607
BanII GRGCYC 2 cut(s) 1641, 2626
BauI CACGAG 1 cut(s) 678
BbrPI CACGTG 1 cut(s) 1118
BbsI GAAGAC 1 cut(s) 482
Bbv12I GWGCWC 6 cut(s) 142, 338, 410, 1449, 2342, 2626
BbvCI CCTCAGC 5 cut(s) 911, 1136, 1254, 1281, 1547
BccI CCATC 6 cut(s) 530, 764, 844, 1069, 1507, 1829
BceAI ACGGC 5 cut(s) 726, 1488, 2231, 2348, 2376
BcgI CGANNNNNNTGC 2 cut(s) 1982, 2016
BciT130I CCWGG 7 cut(s) 65, 417, 1277, 2247, 2411, 2552, 2576
BclI TGATCA 1 cut(s) 2605
BcnI CCSGG 2 cut(s) 767, 1626
BcoDI GTCTC 8 cut(s) 461, 681, 759, 768, 1482, 1577, 1703, 2535
BfaI CTAG 5 cut(s) 653, 2073, 2130, 2499, 2504
BfmI CTRYAG 3 cut(s) 359, 882, 1527
BfoI RGCGCY 2 cut(s) 611, 1398
BglI GCCNNNNNGGC 1 cut(s) 428
BlnI CCTAGG 1 cut(s) 2072
BlpI GCTNAGC 2 cut(s) 1296, 2256
BmcAI AGTACT 1 cut(s) 169
Bme1390I CCNGG 9 cut(s) 65, 417, 767, 1277, 1626, 2247, 2411, 2552, 2576
Bme18I GGWCC 3 cut(s) 620, 1758, 2420
BmeT110I CYCGRG 1 cut(s) 2287
BmgBI CACGTC 1 cut(s) 327
BmgT120I GGNCC 5 cut(s) 67, 620, 1435, 1758, 2420
BmiI GGNNCC 7 cut(s) 414, 421, 609, 1437, 1759, 2123, 2416
BmrFI CCNGG 9 cut(s) 65, 417, 767, 1277, 1626, 2247, 2411, 2552, 2576
BmrI ACTGGG 1 cut(s) 578
BmsI GCATC 7 cut(s) 14, 228, 440, 616, 850, 1195, 1360
BmuI ACTGGG 1 cut(s) 578
BpiI GAAGAC 1 cut(s) 482
BplI GAGNNNNNCTC 2 cut(s) 325, 357
BpmI CTGGAG 3 cut(s) 424, 818, 1053
Bpu10I CCTNAGC 5 cut(s) 911, 1136, 1254, 1281, 1547
Bpu1102I GCTNAGC 2 cut(s) 1296, 2256
BpuEI CTTGAG 2 cut(s) 860, 2560
BpuMI CCSGG 2 cut(s) 767, 1626
BsaAI YACGTR 1 cut(s) 1118
BsaBI GATNNNNATC 1 cut(s) 630
BsaHI GRCGYC 4 cut(s) 255, 608, 1156, 1491
BsaI GGTCTC 2 cut(s) 461, 759
BsaJI CCNNGG 8 cut(s) 423, 833, 1125, 1428, 1578, 2072, 2410, 2575
BsaWI WCCGGW 5 cut(s) 97, 565, 1610, 2387, 2417
BsaXI ACNNNNNCTCC 6 cut(s) 433, 463, 560, 590, 2288, 2318
Bsc4I CCNNNNNNNGG 7 cut(s) 617, 1076, 1091, 1282, 1814, 2078, 2293
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 2 cut(s) 153, 584
Bse3DI GCAATG 1 cut(s) 1330
Bse8I GATNNNNATC 1 cut(s) 630
BseAI TCCGGA 2 cut(s) 565, 2387
BseBI CCWGG 7 cut(s) 65, 417, 1277, 2247, 2411, 2552, 2576
BseDI CCNNGG 8 cut(s) 423, 833, 1125, 1428, 1578, 2072, 2410, 2575
BseGI GGATG 6 cut(s) 60, 431, 775, 1499, 1654, 2586
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 7 cut(s) 617, 1076, 1091, 1282, 1814, 2078, 2293
BseMI GCAATG 1 cut(s) 1330
BseNI ACTGG 2 cut(s) 153, 584
BseRI GAGGAG 5 cut(s) 539, 1364, 2312, 2315, 2616
BseSI GKGCMC 2 cut(s) 1449, 2342
BseX3I CGGCCG 1 cut(s) 2361
BseYI CCCAGC 1 cut(s) 937
BsgI GTGCAG 3 cut(s) 1997, 2127, 2226
Bsh1236I CGCG 4 cut(s) 425, 1632, 2146, 2447
Bsh1285I CGRYCG 2 cut(s) 1630, 2364
BshNI GGYRCC 2 cut(s) 419, 607
BsiEI CGRYCG 2 cut(s) 1630, 2364
BsiHKAI GWGCWC 6 cut(s) 142, 338, 410, 1449, 2342, 2626
BsiHKCI CYCGRG 1 cut(s) 2287
BsiSI CCGG 8 cut(s) 98, 153, 566, 767, 1611, 1626, 2388, 2418
BslFI GGGAC 7 cut(s) 203, 566, 1608, 1771, 2003, 2183, 2270
BslI CCNNNNNNNGG 7 cut(s) 617, 1076, 1091, 1282, 1814, 2078, 2293
BsmAI GTCTC 8 cut(s) 461, 681, 759, 768, 1482, 1577, 1703, 2535
BsmBI CGTCTC 1 cut(s) 1482
BsmFI GGGAC 7 cut(s) 203, 566, 1608, 1771, 2003, 2183, 2270
Bso31I GGTCTC 2 cut(s) 461, 759
BsoBI CYCGRG 1 cut(s) 2287
Bsp1286I GDGCHC 7 cut(s) 142, 338, 410, 1449, 1641, 2342, 2626
Bsp13I TCCGGA 2 cut(s) 565, 2387
Bsp143I GATC 6 cut(s) 160, 373, 1235, 1355, 1827, 2605
Bsp1720I GCTNAGC 2 cut(s) 1296, 2256
Bsp19I CCATGG 1 cut(s) 833
Bsp68I TCGCGA 1 cut(s) 1632
BspACI CCGC 7 cut(s) 423, 425, 871, 1039, 1551, 2447, 2572
BspEI TCCGGA 2 cut(s) 565, 2387
BspFNI CGCG 4 cut(s) 425, 1632, 2146, 2447
BspHI TCATGA 2 cut(s) 961, 2611
BspLI GGNNCC 7 cut(s) 414, 421, 609, 1437, 1759, 2123, 2416
BspMAI CTGCAG 2 cut(s) 363, 886
BspPI GGATC 3 cut(s) 381, 1243, 1822
BspT107I GGYRCC 2 cut(s) 419, 607
BspTNI GGTCTC 2 cut(s) 461, 759
BsrBI CCGCTC 1 cut(s) 1039
BsrDI GCAATG 1 cut(s) 1330
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 2 cut(s) 153, 584
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 8 cut(s) 423, 833, 1125, 1428, 1578, 2072, 2410, 2575
BssMI GATC 6 cut(s) 160, 373, 1235, 1355, 1827, 2605
BssNI GRCGYC 4 cut(s) 255, 608, 1156, 1491
BssSI CACGAG 1 cut(s) 678
BssT1I CCWWGG 5 cut(s) 833, 1125, 1428, 1578, 2072
Bst2BI CACGAG 1 cut(s) 678
Bst2UI CCWGG 7 cut(s) 65, 417, 1277, 2247, 2411, 2552, 2576
Bst4CI ACNGT 7 cut(s) 412, 985, 1714, 1984, 2207, 2284, 2465
Bst6I CTCTTC 3 cut(s) 1991, 2233, 2510
BstACI GRCGYC 4 cut(s) 255, 608, 1156, 1491
BstBAI YACGTR 1 cut(s) 1118
BstC8I GCNNGC 4 cut(s) 503, 1104, 1789, 2647
BstDSI CCRYGG 2 cut(s) 423, 833
BstENI CCTNNNNNAGG 1 cut(s) 1089
BstF5I GGATG 6 cut(s) 60, 431, 775, 1499, 1654, 2586
BstFNI CGCG 4 cut(s) 425, 1632, 2146, 2447
BstH2I RGCGCY 2 cut(s) 611, 1398
BstHHI GCGC 4 cut(s) 507, 610, 1397, 2146
BstKTI GATC 6 cut(s) 163, 376, 1238, 1358, 1830, 2608
BstMAI GTCTC 8 cut(s) 461, 681, 759, 768, 1482, 1577, 1703, 2535
BstMBI GATC 6 cut(s) 160, 373, 1235, 1355, 1827, 2605
BstMCI CGRYCG 2 cut(s) 1630, 2364
BstNI CCWGG 7 cut(s) 65, 417, 1277, 2247, 2411, 2552, 2576
BstNSI RCATGY 3 cut(s) 1314, 1590, 2279
BstSCI CCNGG 9 cut(s) 63, 415, 765, 1275, 1624, 2245, 2409, 2550, 2574
BstSFI CTRYAG 3 cut(s) 359, 882, 1527
BstSLI GKGCMC 2 cut(s) 1449, 2342
BstUI CGCG 4 cut(s) 425, 1632, 2146, 2447
BstV2I GAAGAC 1 cut(s) 482
BstX2I RGATCY 1 cut(s) 1827
BstYI RGATCY 1 cut(s) 1827
BstZI CGGCCG 1 cut(s) 2361
BtgI CCRYGG 2 cut(s) 423, 833
BtgZI GCGATG 1 cut(s) 1647
BtrI CACGTC 1 cut(s) 327
BtsCI GGATG 6 cut(s) 60, 431, 775, 1499, 1654, 2586
BtsI GCAGTG 1 cut(s) 1825
BtsIMutI CAGTG 1 cut(s) 1825
BtuMI TCGCGA 1 cut(s) 1632
Cac8I GCNNGC 4 cut(s) 503, 1104, 1789, 2647
CciI TCATGA 2 cut(s) 961, 2611
CfoI GCGC 4 cut(s) 507, 610, 1397, 2146
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 5 cut(s) 67, 620, 1435, 1758, 2420
Cfr42I CCGCGG 1 cut(s) 426
CseI GACGC 4 cut(s) 244, 1164, 1755, 2069
CsiI ACCWGGT 1 cut(s) 2245
Csp6I GTAC 4 cut(s) 168, 830, 932, 2535
CspCI CAANNNNNGTGG 2 cut(s) 293, 328
CviQI GTAC 4 cut(s) 168, 830, 932, 2535
DinI GGCGCC 1 cut(s) 609
DpnI GATC 6 cut(s) 162, 375, 1237, 1357, 1829, 2607
DpnII GATC 6 cut(s) 160, 373, 1235, 1355, 1827, 2605
DraI TTTAAA 1 cut(s) 1564
DrdI GACNNNNNNGTC 2 cut(s) 1765, 2499
DseDI GACNNNNNNGTC 2 cut(s) 1765, 2499
EaeI YGGCCR 4 cut(s) 150, 1473, 1837, 2361
EagI CGGCCG 1 cut(s) 2361
Eam1104I CTCTTC 3 cut(s) 1991, 2233, 2510
EarI CTCTTC 3 cut(s) 1991, 2233, 2510
EciI GGCGGA 1 cut(s) 2561
Ecl136II GAGCTC 1 cut(s) 2624
EclXI CGGCCG 1 cut(s) 2361
Eco130I CCWWGG 5 cut(s) 833, 1125, 1428, 1578, 2072
Eco24I GRGCYC 2 cut(s) 1641, 2626
Eco31I GGTCTC 2 cut(s) 461, 759
Eco47I GGWCC 3 cut(s) 620, 1758, 2420
Eco52I CGGCCG 1 cut(s) 2361
Eco53kI GAGCTC 1 cut(s) 2624
Eco57I CTGAAG 3 cut(s) 27, 56, 2468
Eco72I CACGTG 1 cut(s) 1118
Eco88I CYCGRG 1 cut(s) 2287
EcoICRI GAGCTC 1 cut(s) 2624
EcoNI CCTNNNNNAGG 1 cut(s) 1089
EcoO109I RGGNCCY 2 cut(s) 67, 1435
EcoRII CCWGG 7 cut(s) 63, 415, 1275, 2245, 2409, 2550, 2574
EcoT14I CCWWGG 5 cut(s) 833, 1125, 1428, 1578, 2072
EcoT38I GRGCYC 2 cut(s) 1641, 2626
EgeI GGCGCC 1 cut(s) 609
EheI GGCGCC 1 cut(s) 609
ErhI CCWWGG 5 cut(s) 833, 1125, 1428, 1578, 2072
Esp3I CGTCTC 1 cut(s) 1482
FalI AAGNNNNNCTT 4 cut(s) 1776, 1808, 2047, 2079
FaqI GGGAC 7 cut(s) 203, 566, 1608, 1771, 2003, 2183, 2270
FauNDI CATATG 1 cut(s) 1111
FbaI TGATCA 1 cut(s) 2605
FokI GGATG 6 cut(s) 47, 418, 782, 1486, 1661, 2593
FriOI GRGCYC 2 cut(s) 1641, 2626
FspBI CTAG 5 cut(s) 653, 2073, 2130, 2499, 2504
GlaI GCGC 4 cut(s) 506, 609, 1396, 2145
GsaI CCCAGC 1 cut(s) 941
GsuI CTGGAG 3 cut(s) 424, 818, 1053
HaeII RGCGCY 2 cut(s) 611, 1398
HapII CCGG 8 cut(s) 98, 153, 566, 767, 1611, 1626, 2388, 2418
HgaI GACGC 4 cut(s) 244, 1164, 1755, 2069
HhaI GCGC 4 cut(s) 507, 610, 1397, 2146
Hin1I GRCGYC 4 cut(s) 255, 608, 1156, 1491
Hin6I GCGC 4 cut(s) 505, 608, 1395, 2144
HinP1I GCGC 4 cut(s) 505, 608, 1395, 2144
HpaII CCGG 8 cut(s) 98, 153, 566, 767, 1611, 1626, 2388, 2418
HphI GGTGA 6 cut(s) 59, 167, 321, 866, 1106, 2093
Hpy166II GTNNAC 3 cut(s) 1447, 2340, 2358
Hpy188I TCNGA 8 cut(s) 207, 224, 747, 781, 1071, 1648, 2487, 2605
Hpy8I GTNNAC 3 cut(s) 1447, 2340, 2358
Hpy99I CGWCG 1 cut(s) 260
HpyCH4III ACNGT 7 cut(s) 412, 985, 1714, 1984, 2207, 2284, 2465
HpyCH4IV ACGT 4 cut(s) 326, 1117, 1290, 1491
HpySE526I ACGT 4 cut(s) 326, 1117, 1290, 1491
Hsp92I GRCGYC 4 cut(s) 255, 608, 1156, 1491
HspAI GCGC 4 cut(s) 505, 608, 1395, 2144
KasI GGCGCC 1 cut(s) 607
Kpn2I TCCGGA 2 cut(s) 565, 2387
Ksp22I TGATCA 1 cut(s) 2605
KspI CCGCGG 1 cut(s) 426
Kzo9I GATC 6 cut(s) 160, 373, 1235, 1355, 1827, 2605
LmnI GCTCC 5 cut(s) 145, 405, 799, 2050, 2629
LweI GCATC 7 cut(s) 14, 228, 440, 616, 850, 1195, 1360
MabI ACCWGGT 1 cut(s) 2245
MaeI CTAG 5 cut(s) 653, 2073, 2130, 2499, 2504
MaeII ACGT 4 cut(s) 326, 1117, 1290, 1491
MaeIII GTNAC 8 cut(s) 322, 327, 1820, 2057, 2081, 2211, 2278, 2434
MalI GATC 6 cut(s) 162, 375, 1237, 1357, 1829, 2607
MbiI CCGCTC 1 cut(s) 1039
MboI GATC 6 cut(s) 160, 373, 1235, 1355, 1827, 2605
MfeI CAATTG 3 cut(s) 349, 1656, 1770
MflI RGATCY 1 cut(s) 1827
MhlI GDGCHC 7 cut(s) 142, 338, 410, 1449, 1641, 2342, 2626
MlsI TGGCCA 1 cut(s) 1839
MluNI TGGCCA 1 cut(s) 1839
Mly113I GGCGCC 1 cut(s) 608
MlyI GAGTC 8 cut(s) 36, 436, 472, 683, 770, 1053, 1760, 2609
Mox20I TGGCCA 1 cut(s) 1839
MroI TCCGGA 2 cut(s) 565, 2387
MscI TGGCCA 1 cut(s) 1839
MseI TTAA 6 cut(s) 384, 1248, 1563, 1679, 2067, 2223
MslI CAYNNNNRTG 2 cut(s) 10, 2363
Msp20I TGGCCA 1 cut(s) 1839
MspA1I CMGCKG 5 cut(s) 320, 425, 871, 1106, 1551
MspI CCGG 8 cut(s) 98, 153, 566, 767, 1611, 1626, 2388, 2418
MspR9I CCNGG 9 cut(s) 65, 417, 767, 1277, 1626, 2247, 2411, 2552, 2576
MunI CAATTG 3 cut(s) 349, 1656, 1770
MvaI CCWGG 7 cut(s) 65, 417, 1277, 2247, 2411, 2552, 2576
MvnI CGCG 4 cut(s) 425, 1632, 2146, 2447
NarI GGCGCC 1 cut(s) 608
NciI CCSGG 2 cut(s) 767, 1626
NcoI CCATGG 1 cut(s) 833
NdeI CATATG 1 cut(s) 1111
NdeII GATC 6 cut(s) 160, 373, 1235, 1355, 1827, 2605
NlaIV GGNNCC 7 cut(s) 414, 421, 609, 1437, 1759, 2123, 2416
NmuCI GTSAC 5 cut(s) 322, 327, 1820, 2057, 2081
NruI TCGCGA 1 cut(s) 1632
NspI RCATGY 3 cut(s) 1314, 1590, 2279
OliI CACNNNNGTG 1 cut(s) 2363
PagI TCATGA 2 cut(s) 961, 2611
PciI ACATGT 1 cut(s) 2275
PfeI GAWTC 6 cut(s) 50, 90, 640, 1028, 1211, 1388
PflFI GACNNNGTC 1 cut(s) 2087
PfoI TCCNGGA 1 cut(s) 2550
PleI GAGTC 8 cut(s) 35, 436, 472, 682, 770, 1052, 1759, 2608
PluTI GGCGCC 1 cut(s) 611
PmaCI CACGTG 1 cut(s) 1118
PmlI CACGTG 1 cut(s) 1118
PpsI GAGTC 8 cut(s) 35, 436, 472, 682, 770, 1052, 1759, 2608
Ppu21I YACGTR 1 cut(s) 1118
PscI ACATGT 1 cut(s) 2275
PshBI ATTAAT 1 cut(s) 1679
Psp124BI GAGCTC 1 cut(s) 2626
Psp1406I AACGTT 1 cut(s) 1290
Psp6I CCWGG 7 cut(s) 63, 415, 1275, 2245, 2409, 2550, 2574
PspCI CACGTG 1 cut(s) 1118
PspFI CCCAGC 1 cut(s) 937
PspGI CCWGG 7 cut(s) 63, 415, 1275, 2245, 2409, 2550, 2574
PspN4I GGNNCC 7 cut(s) 414, 421, 609, 1437, 1759, 2123, 2416
PspPI GGNCC 5 cut(s) 67, 620, 1435, 1758, 2420
PstI CTGCAG 2 cut(s) 363, 886
PsuI RGATCY 1 cut(s) 1827
PsyI GACNNNGTC 1 cut(s) 2087
PvuII CAGCTG 2 cut(s) 320, 1106
RruI TCGCGA 1 cut(s) 1632
RsaI GTAC 4 cut(s) 169, 831, 933, 2536
RsaNI GTAC 4 cut(s) 168, 830, 932, 2535
RseI CAYNNNNRTG 2 cut(s) 10, 2363
SacI GAGCTC 1 cut(s) 2626
SacII CCGCGG 1 cut(s) 426
SaqAI TTAA 6 cut(s) 384, 1248, 1563, 1679, 2067, 2223
Sau3AI GATC 6 cut(s) 160, 373, 1235, 1355, 1827, 2605
Sau96I GGNCC 5 cut(s) 67, 620, 1435, 1758, 2420
ScaI AGTACT 1 cut(s) 169
SchI GAGTC 8 cut(s) 36, 436, 472, 683, 770, 1053, 1760, 2609
ScrFI CCNGG 9 cut(s) 65, 417, 767, 1277, 1626, 2247, 2411, 2552, 2576
SduI GDGCHC 7 cut(s) 142, 338, 410, 1449, 1641, 2342, 2626
SexAI ACCWGGT 1 cut(s) 2245
SfaNI GCATC 7 cut(s) 14, 228, 440, 616, 850, 1195, 1360
SfcI CTRYAG 3 cut(s) 359, 882, 1527
SfoI GGCGCC 1 cut(s) 609
Sfr303I CCGCGG 1 cut(s) 426
SgrBI CCGCGG 1 cut(s) 426
SinI GGWCC 3 cut(s) 620, 1758, 2420
SmiMI CAYNNNNRTG 2 cut(s) 10, 2363
SmlI CTYRAG 2 cut(s) 875, 2539
SmoI CTYRAG 2 cut(s) 875, 2539
SsiI CCGC 7 cut(s) 423, 425, 871, 1039, 1551, 2447, 2572
SspDI GGCGCC 1 cut(s) 607
SspMI CTAG 5 cut(s) 653, 2073, 2130, 2499, 2504
SstI GAGCTC 1 cut(s) 2626
StyD4I CCNGG 9 cut(s) 63, 415, 765, 1275, 1624, 2245, 2409, 2550, 2574
StyI CCWWGG 5 cut(s) 833, 1125, 1428, 1578, 2072
TaaI ACNGT 7 cut(s) 412, 985, 1714, 1984, 2207, 2284, 2465
TaiI ACGT 4 cut(s) 329, 1120, 1293, 1494
TaqI TCGA 2 cut(s) 522, 1055
TatI WGTACW 1 cut(s) 167
TauI GCSGC 2 cut(s) 425, 874
TfiI GAWTC 6 cut(s) 50, 90, 640, 1028, 1211, 1388
Tru1I TTAA 6 cut(s) 384, 1248, 1563, 1679, 2067, 2223
Tru9I TTAA 6 cut(s) 384, 1248, 1563, 1679, 2067, 2223
TscAI CASTG 1 cut(s) 1825
TseFI GTSAC 5 cut(s) 322, 327, 1820, 2057, 2081
Tsp45I GTSAC 5 cut(s) 322, 327, 1820, 2057, 2081
TspDTI ATGAA 4 cut(s) 488, 950, 1665, 1874
TspGWI ACGGA 2 cut(s) 686, 1740
TspRI CASTG 1 cut(s) 1825
Tth111I GACNNNGTC 1 cut(s) 2087
VneI GTGCAC 2 cut(s) 1445, 2338
VpaK11BI GGWCC 3 cut(s) 620, 1758, 2420
VspI ATTAAT 1 cut(s) 1679
XagI CCTNNNNNAGG 1 cut(s) 1089
XapI RAATTY 6 cut(s) 946, 970, 1887, 1897, 1967, 2396
XbaI TCTAGA 1 cut(s) 2503
XceI RCATGY 3 cut(s) 1314, 1590, 2279
XmaJI CCTAGG 1 cut(s) 2072
XspI CTAG 5 cut(s) 653, 2073, 2130, 2499, 2504
ZraI GACGTC 1 cut(s) 1492
ZrmI AGTACT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.