Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0013892

Physical Location & Seq

Genomic Coordinates Forward (+)

6229986 .. 6233706

Gene Structure 3721 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g11030.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1395 bp

ATGGAGTTGTTCTACTTTCTTCTGTTCGGTGCTCTCTCGGCCGTGGTGGCAGCTTTGGAGCTGAGCAAGAGGAACAAGGATCGCATTATTACCTCCTCCACTTTCACACTCTTCAAGAACAATTATCTCCTCGTTTATTCTCTCATGATGGCCGGGGATTGGTTGCAGGGTCCATATGTTTACTACCTCTACAGCACTTATGGGTTTACAAAGGGCGATATTGGACAACTCTTTATTGCTGGGTTTGGTTCGTCTATGTTGTTTGGGACAATTGTAGGGTCTCTAGCTGACAAACAGGGTCGGAAGAGGGCATGTGTTACGTACTGCATAACTTACATCCTAAGTTGCTTCACCAAGCATTTTCCTGACTACAAAGTTTTAATGTTGGGACGCGTACTAGGAGGCATTGCAACTTCTCTACTTTTTTCAGCTTTTGAGTCTTGGCTTGTTGCAGAACACAATAAGAGGGGCTTTGAACAACAATGGCTTTCACTCACATTCTCTAAGGCCATTTTTCTTGGTAACGGTCTGATTGCTATTGTATCTGGGTTATTGGGGAATCTTCTGGTAGATACTCTGGCATTCGGTCCAGTTGCTCCATTTGATGCTGCTGCCTGCATTTTGGCAATTGGCATGGCAATCATATTGGCAACATGGGGTGAAAACTATGGAGACTCATCAGATAACAAGAACTTGCTGACCCAGTTCAGGGGTGCTGCTATAGCCATTGCTTGTGATGAGAAAATCGCTTTGCTGGGTGCCATACAGTCCTTATTTGAAGGCTCAATGTATACCTTTGTGTTTCTCTGGACTCCTGCTCTGAGCCCAAATGATGAGGAGATTCCACATGGTTTCATTTTTGCAACATTCATGCTAGCGTCAATGTTGGGGAGCTCTATTGCATCTCGATTGATGGCCAGCTCAGCCCCCAGAGTCGAGAGCTACATGCAGATTGTATTTGCAGTTTCTGCAGCATCCCTTCTGTGCCCCATTGTGACTAGTTTCTTGATTGCACCTTCCAATGTGAGAGGTGGGGGCATCTCGTTCTCAGGGTATTTTCAAATTTTTGGATTCTGCACATTTGAGGCCTGTGTGGGGATATTCTGGCCATCTATCATGAAAATGAGATCACAATACATCCCAGAGGAGGCACGAAGCACCATCATGAACTTCTTTCGCATTCCTCTCAACATTTTTGTGTGTGTAGTGCTGTACAATGTCAATGCATTTCCCATCACCATCATGTTCGGTATGTGCTCGATTTTCCTCGGTACGGCTGCTGTGTTGCAGAGACGGCTCTTTGTGATAACTGAGGGTGGCAGCCATAAGTCAAAATCATCACTAGAATGGATACCAATGGAGATGGACACTGAAGCGGAGCCATTAAATGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0007154 GO:0007165 GO:0008150 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009941 GO:0009987 GO:0016020 GO:0019867 GO:0019899 GO:0023052 GO:0031090 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0038023 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051087 GO:0051117 GO:0051716 GO:0060089 GO:0065007 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFS_5

Protein Analysis

465

Amino Acids

51.01

Weight (kDa)

6.14

Isoelectric Point (pI)

38.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_5	PF05631	4 - 358	2.6e-180	Sugar-tranasporters, 12 TM
MFS_1	PF07690	43 - 393	7.6e-16	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013892)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64650 AT1G64650
fragaria_vesca	FvH4_5g11030 FvH4_5g11030 FvH4_5g11030
malus_domestica	MD06G1117300.v1.1
prunus_persica	Prupe.5G128400_v2.0.a1 Prupe.5G128400_v2.0.a1
pyrus_communis	pycom06g10920
rosa_chinensis	RchiOBHm_Chr7g0186771
rosa_laevigata	RLG00000004817
rosa_multiflora	Rmu_sc0009734.1_g000002
rosa_roxburghii	Rroxscaffold_3G00267840
rosa_rugosa	Rorug06G0476500
rosa_samantha	Rh7BG080500 Rh7CG083500
rosa_wichuraiana	Rw7G007240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	758
AccI	GTMKAC	1 cut(s)	791
AccII	CGCG	1 cut(s)	393
AciI	CCGC	1 cut(s)	1376
AclWI	GGATC	1 cut(s)	87
AcoI	YGGCCR	4 cut(s)	39, 150, 915, 1106
AcsI	RAATTY	1 cut(s)	1062
AcuI	CTGAAG	1 cut(s)	1392
AfaI	GTAC	4 cut(s)	323, 396, 1214, 1273
AfiI	CCNNNNNNNGG	6 cut(s)	159, 708, 709, 1095, 1147, 1273
AflIII	ACRYGT	1 cut(s)	391
AgsI	TTSAA	4 cut(s)	115, 476, 779, 1061
AhlI	ACTAGT	1 cut(s)	998
AluBI	AGCT	7 cut(s)	53, 61, 287, 431, 894, 921, 942
AluI	AGCT	7 cut(s)	53, 61, 287, 431, 894, 921, 942
Alw21I	GWGCWC	3 cut(s)	34, 896, 1259
Alw26I	GTCTC	3 cut(s)	285, 666, 1285
AlwI	GGATC	1 cut(s)	87
AlwNI	CAGNNNCTG	1 cut(s)	968
AoxI	GGCC	6 cut(s)	39, 150, 507, 915, 1086, 1106
ApeKI	GCWGC	7 cut(s)	50, 608, 611, 716, 971, 1277, 1320
ApoI	RAATTY	1 cut(s)	1062
AspS9I	GGNCC	2 cut(s)	170, 587
AsuC2I	CCSGG	1 cut(s)	154
AsuHPI	GGTGA	3 cut(s)	343, 671, 1228
AsuNHI	GCTAGC	1 cut(s)	874
AvaII	GGWCC	2 cut(s)	170, 587
BaeGI	GKGCMC	1 cut(s)	989
BalI	TGGCCA	2 cut(s)	917, 1108
BanI	GGYRCC	1 cut(s)	758
BanII	GRGCYC	2 cut(s)	827, 896
Bbv12I	GWGCWC	3 cut(s)	34, 896, 1259
BbvI	GCAGC	7 cut(s)	62, 595, 598, 703, 983, 1264, 1332
BccI	CCATC	7 cut(s)	142, 907, 1117, 1169, 1241, 1247, 1357
BceAI	ACGGC	3 cut(s)	26, 1290, 1310
BciVI	GTATCC	1 cut(s)	1344
BcnI	CCSGG	1 cut(s)	154
BcoDI	GTCTC	3 cut(s)	285, 666, 1285
BcuI	ACTAGT	1 cut(s)	998
BfaI	CTAG	5 cut(s)	284, 398, 875, 999, 1343
BfmI	CTRYAG	3 cut(s)	190, 720, 969
BfuI	GTATCC	1 cut(s)	1344
BglI	GCCNNNNNGGC	1 cut(s)	47
BisI	GCNGC	7 cut(s)	51, 609, 612, 717, 972, 1278, 1321
BlpI	GCTNAGC	2 cut(s)	62, 922
BlsI	GCNGC	7 cut(s)	52, 610, 613, 718, 973, 1279, 1322
Bme1390I	CCNGG	1 cut(s)	154
Bme18I	GGWCC	2 cut(s)	170, 587
BmgT120I	GGNCC	2 cut(s)	170, 587
BmiI	GGNNCC	3 cut(s)	171, 760, 1380
BmrFI	CCNGG	1 cut(s)	154
BmrI	ACTGGG	1 cut(s)	697
BmsI	GCATC	4 cut(s)	595, 911, 983, 1047
BmtI	GCTAGC	1 cut(s)	878
BmuI	ACTGGG	1 cut(s)	697
Bpu1102I	GCTNAGC	2 cut(s)	62, 922
BpuMI	CCSGG	1 cut(s)	154
BsaAI	YACGTR	1 cut(s)	321
BsaI	GGTCTC	1 cut(s)	285
BsaJI	CCNNGG	3 cut(s)	42, 153, 1267
Bsc4I	CCNNNNNNNGG	6 cut(s)	159, 708, 709, 1095, 1147, 1273
Bse1I	ACTGG	2 cut(s)	590, 703
Bse3DI	GCAATG	2 cut(s)	405, 726
BseDI	CCNNGG	3 cut(s)	42, 153, 1267
BseGI	GGATG	3 cut(s)	336, 974, 1137
BseLI	CCNNNNNNNGG	6 cut(s)	159, 708, 709, 1095, 1147, 1273
BseMI	GCAATG	2 cut(s)	405, 726
BseMII	CTCAG	5 cut(s)	53, 812, 936, 1062, 1302
BseNI	ACTGG	2 cut(s)	590, 703
BseRI	GAGGAG	4 cut(s)	85, 119, 851, 1160
BseSI	GKGCMC	1 cut(s)	989
BseX3I	CGGCCG	1 cut(s)	39
BseXI	GCAGC	7 cut(s)	62, 595, 598, 703, 983, 1264, 1332
BseYI	CCCAGC	2 cut(s)	239, 754
BsgI	GTGCAG	1 cut(s)	1060
Bsh1236I	CGCG	1 cut(s)	393
Bsh1285I	CGRYCG	1 cut(s)	42
BshFI	GGCC	6 cut(s)	41, 152, 509, 917, 1088, 1108
BshNI	GGYRCC	1 cut(s)	758
BsiEI	CGRYCG	1 cut(s)	42
BsiHKAI	GWGCWC	3 cut(s)	34, 896, 1259
BsiSI	CCGG	1 cut(s)	153
BslFI	GGGAC	2 cut(s)	280, 402
BslI	CCNNNNNNNGG	6 cut(s)	159, 708, 709, 1095, 1147, 1273
BsmAI	GTCTC	3 cut(s)	285, 666, 1285
BsmBI	CGTCTC	1 cut(s)	1285
BsmFI	GGGAC	2 cut(s)	280, 402
BsmI	GAATGC	2 cut(s)	581, 1179
BsnI	GGCC	6 cut(s)	41, 152, 509, 917, 1088, 1108
Bso31I	GGTCTC	1 cut(s)	285
Bsp1286I	GDGCHC	5 cut(s)	34, 827, 896, 989, 1259
Bsp1407I	TGTACA	1 cut(s)	1212
Bsp143I	GATC	2 cut(s)	79, 1127
Bsp1720I	GCTNAGC	2 cut(s)	62, 922
BspACI	CCGC	1 cut(s)	1376
BspANI	GGCC	6 cut(s)	41, 152, 509, 917, 1088, 1108
BspCNI	CTCAG	5 cut(s)	54, 813, 935, 1061, 1303
BspFNI	CGCG	1 cut(s)	393
BspHI	TCATGA	3 cut(s)	144, 1116, 1164
BspLI	GGNNCC	3 cut(s)	171, 760, 1380
BspMAI	CTGCAG	1 cut(s)	973
BspOI	GCTAGC	1 cut(s)	878
BspPI	GGATC	1 cut(s)	87
BspT107I	GGYRCC	1 cut(s)	758
BspTNI	GGTCTC	1 cut(s)	285
BsrDI	GCAATG	2 cut(s)	405, 726
BsrGI	TGTACA	1 cut(s)	1212
BsrI	ACTGG	2 cut(s)	590, 703
BssECI	CCNNGG	3 cut(s)	42, 153, 1267
BssMI	GATC	2 cut(s)	79, 1127
BssNAI	GTATAC	1 cut(s)	792
Bst1107I	GTATAC	1 cut(s)	792
Bst4CI	ACNGT	2 cut(s)	527, 768
Bst6I	CTCTTC	2 cut(s)	116, 299
BstAPI	GCANNNNNTGC	1 cut(s)	968
BstAUI	TGTACA	1 cut(s)	1212
BstBAI	YACGTR	1 cut(s)	321
BstC8I	GCNNGC	3 cut(s)	616, 876, 919
BstDEI	CTNAG	7 cut(s)	62, 341, 504, 821, 922, 1048, 1311
BstDSI	CCRYGG	1 cut(s)	42
BstF5I	GGATG	3 cut(s)	336, 974, 1137
BstFNI	CGCG	1 cut(s)	393
BstKTI	GATC	2 cut(s)	82, 1130
BstMAI	GTCTC	3 cut(s)	285, 666, 1285
BstMBI	GATC	2 cut(s)	79, 1127
BstMCI	CGRYCG	1 cut(s)	42
BstMWI	GCNNNNNNNGC	6 cut(s)	38, 47, 722, 923, 968, 1294
BstNSI	RCATGY	2 cut(s)	315, 949
BstSCI	CCNGG	1 cut(s)	152
BstSFI	CTRYAG	3 cut(s)	190, 720, 969
BstSLI	GKGCMC	1 cut(s)	989
BstSNI	TACGTA	1 cut(s)	321
BstUI	CGCG	1 cut(s)	393
BstV1I	GCAGC	7 cut(s)	62, 595, 598, 703, 983, 1264, 1332
BstZ17I	GTATAC	1 cut(s)	792
BstZI	CGGCCG	1 cut(s)	39
BsuI	GTATCC	1 cut(s)	1344
BsuRI	GGCC	6 cut(s)	41, 152, 509, 917, 1088, 1108
BtgI	CCRYGG	1 cut(s)	42
BtsCI	GGATG	3 cut(s)	336, 974, 1137
BtsIMutI	CAGTG	1 cut(s)	1368
Cac8I	GCNNGC	3 cut(s)	616, 876, 919
CaiI	CAGNNNCTG	1 cut(s)	968
CciI	TCATGA	3 cut(s)	144, 1116, 1164
Cfr13I	GGNCC	2 cut(s)	170, 587
CseI	GACGC	2 cut(s)	399, 867
Csp6I	GTAC	4 cut(s)	322, 395, 1213, 1272
CviQI	GTAC	4 cut(s)	322, 395, 1213, 1272
DdeI	CTNAG	7 cut(s)	62, 341, 504, 821, 922, 1048, 1311
DpnI	GATC	2 cut(s)	81, 1129
DpnII	GATC	2 cut(s)	79, 1127
EaeI	YGGCCR	4 cut(s)	39, 150, 915, 1106
EagI	CGGCCG	1 cut(s)	39
Eam1104I	CTCTTC	2 cut(s)	116, 299
EarI	CTCTTC	2 cut(s)	116, 299
Ecl136II	GAGCTC	1 cut(s)	894
EclXI	CGGCCG	1 cut(s)	39
Eco105I	TACGTA	1 cut(s)	321
Eco147I	AGGCCT	1 cut(s)	1088
Eco24I	GRGCYC	2 cut(s)	827, 896
Eco31I	GGTCTC	1 cut(s)	285
Eco47I	GGWCC	2 cut(s)	170, 587
Eco52I	CGGCCG	1 cut(s)	39
Eco53kI	GAGCTC	1 cut(s)	894
Eco57I	CTGAAG	1 cut(s)	1392
EcoICRI	GAGCTC	1 cut(s)	894
EcoT22I	ATGCAT	1 cut(s)	1228
EcoT38I	GRGCYC	2 cut(s)	827, 896
Esp3I	CGTCTC	1 cut(s)	1285
FalI	AAGNNNNNCTT	2 cut(s)	455, 487
FaqI	GGGAC	2 cut(s)	280, 402
FauNDI	CATATG	1 cut(s)	175
FblI	GTMKAC	1 cut(s)	791
Fnu4HI	GCNGC	7 cut(s)	51, 609, 612, 717, 972, 1278, 1321
FokI	GGATG	3 cut(s)	323, 961, 1124
FriOI	GRGCYC	2 cut(s)	827, 896
Fsp4HI	GCNGC	7 cut(s)	51, 609, 612, 717, 972, 1278, 1321
FspBI	CTAG	5 cut(s)	284, 398, 875, 999, 1343
GluI	GCNGC	7 cut(s)	51, 609, 612, 717, 972, 1278, 1321
GsaI	CCCAGC	2 cut(s)	243, 758
HaeIII	GGCC	6 cut(s)	41, 152, 509, 917, 1088, 1108
HapII	CCGG	1 cut(s)	153
HgaI	GACGC	2 cut(s)	399, 867
HinfI	GANTC	7 cut(s)	437, 559, 674, 811, 841, 933, 1071
HpaII	CCGG	1 cut(s)	153
HphI	GGTGA	3 cut(s)	343, 671, 1228
Hpy166II	GTNNAC	3 cut(s)	181, 207, 792
Hpy188I	TCNGA	4 cut(s)	303, 531, 682, 822
Hpy188III	TCNNGA	9 cut(s)	115, 145, 365, 808, 906, 937, 1006, 1117, 1165
Hpy8I	GTNNAC	3 cut(s)	181, 207, 792
HpyAV	CCTTC	3 cut(s)	773, 989, 1026
HpyCH4III	ACNGT	2 cut(s)	527, 768
HpyCH4IV	ACGT	1 cut(s)	320
HpyF10VI	GCNNNNNNNGC	6 cut(s)	38, 47, 722, 923, 968, 1294
HpyF3I	CTNAG	7 cut(s)	62, 341, 504, 821, 922, 1048, 1311
HpySE526I	ACGT	1 cut(s)	320
Kzo9I	GATC	2 cut(s)	79, 1127
LmnI	GCTCC	4 cut(s)	58, 601, 891, 1378
Lsp1109I	GCAGC	7 cut(s)	62, 595, 598, 703, 983, 1264, 1332
LweI	GCATC	4 cut(s)	595, 911, 983, 1047
MaeI	CTAG	5 cut(s)	284, 398, 875, 999, 1343
MaeII	ACGT	1 cut(s)	320
MaeIII	GTNAC	3 cut(s)	316, 521, 994
MalI	GATC	2 cut(s)	81, 1129
MboI	GATC	2 cut(s)	79, 1127
MboII	GAAGA	4 cut(s)	11, 103, 316, 554
MfeI	CAATTG	2 cut(s)	270, 627
MhlI	GDGCHC	5 cut(s)	34, 827, 896, 989, 1259
MlsI	TGGCCA	2 cut(s)	917, 1108
MluCI	AATT	4 cut(s)	121, 270, 627, 1062
MluI	ACGCGT	1 cut(s)	391
MluNI	TGGCCA	2 cut(s)	917, 1108
MlyI	GAGTC	4 cut(s)	446, 668, 805, 942
MmeI	TCCRAC	1 cut(s)	281
Mox20I	TGGCCA	2 cut(s)	917, 1108
Mph1103I	ATGCAT	1 cut(s)	1228
MscI	TGGCCA	2 cut(s)	917, 1108
MseI	TTAA	2 cut(s)	380, 1385
MslI	CAYNNNNRTG	5 cut(s)	1121, 1163, 1196, 1241, 1345
Msp20I	TGGCCA	2 cut(s)	917, 1108
MspI	CCGG	1 cut(s)	153
MspR9I	CCNGG	1 cut(s)	154
MunI	CAATTG	2 cut(s)	270, 627
Mva1269I	GAATGC	2 cut(s)	581, 1179
MvnI	CGCG	1 cut(s)	393
MwoI	GCNNNNNNNGC	6 cut(s)	38, 47, 722, 923, 968, 1294
NciI	CCSGG	1 cut(s)	154
NdeI	CATATG	1 cut(s)	175
NdeII	GATC	2 cut(s)	79, 1127
NheI	GCTAGC	1 cut(s)	874
NlaIV	GGNNCC	3 cut(s)	171, 760, 1380
NmeAIII	GCCGAG	1 cut(s)	17
NmuCI	GTSAC	1 cut(s)	994
NsiI	ATGCAT	1 cut(s)	1228
NspI	RCATGY	2 cut(s)	315, 949
PagI	TCATGA	3 cut(s)	144, 1116, 1164
PceI	AGGCCT	1 cut(s)	1088
PctI	GAATGC	2 cut(s)	581, 1179
PfeI	GAWTC	3 cut(s)	559, 841, 1071
PkrI	GCNGC	7 cut(s)	52, 610, 613, 718, 973, 1279, 1322
PleI	GAGTC	4 cut(s)	445, 668, 805, 941
PpsI	GAGTC	4 cut(s)	445, 668, 805, 941
Ppu21I	YACGTR	1 cut(s)	321
Psp124BI	GAGCTC	1 cut(s)	896
PspFI	CCCAGC	2 cut(s)	239, 754
PspN4I	GGNNCC	3 cut(s)	171, 760, 1380
PspPI	GGNCC	2 cut(s)	170, 587
PstI	CTGCAG	1 cut(s)	973
PstNI	CAGNNNCTG	1 cut(s)	968
RsaI	GTAC	4 cut(s)	323, 396, 1214, 1273
RsaNI	GTAC	4 cut(s)	322, 395, 1213, 1272
RseI	CAYNNNNRTG	5 cut(s)	1121, 1163, 1196, 1241, 1345
SacI	GAGCTC	1 cut(s)	896
SaqAI	TTAA	2 cut(s)	380, 1385
SatI	GCNGC	7 cut(s)	51, 609, 612, 717, 972, 1278, 1321
Sau3AI	GATC	2 cut(s)	79, 1127
Sau96I	GGNCC	2 cut(s)	170, 587
SchI	GAGTC	4 cut(s)	446, 668, 805, 942
ScrFI	CCNGG	1 cut(s)	154
SduI	GDGCHC	5 cut(s)	34, 827, 896, 989, 1259
SfaNI	GCATC	4 cut(s)	595, 911, 983, 1047
SfcI	CTRYAG	3 cut(s)	190, 720, 969
SinI	GGWCC	2 cut(s)	170, 587
SmiMI	CAYNNNNRTG	5 cut(s)	1121, 1163, 1196, 1241, 1345
SnaBI	TACGTA	1 cut(s)	321
SpeI	ACTAGT	1 cut(s)	998
Sse9I	AATT	4 cut(s)	121, 270, 627, 1062
SseBI	AGGCCT	1 cut(s)	1088
SsiI	CCGC	1 cut(s)	1376
SspMI	CTAG	5 cut(s)	284, 398, 875, 999, 1343
SstI	GAGCTC	1 cut(s)	896
StuI	AGGCCT	1 cut(s)	1088
StyD4I	CCNGG	1 cut(s)	152
TaaI	ACNGT	2 cut(s)	527, 768
TaiI	ACGT	1 cut(s)	323
TaqI	TCGA	3 cut(s)	907, 936, 1259
TaqII	GACCGA	1 cut(s)	575
TasI	AATT	4 cut(s)	121, 270, 627, 1062
TatI	WGTACW	1 cut(s)	1212
TfiI	GAWTC	3 cut(s)	559, 841, 1071
Tru1I	TTAA	2 cut(s)	380, 1385
Tru9I	TTAA	2 cut(s)	380, 1385
TscAI	CASTG	1 cut(s)	1375
TseFI	GTSAC	1 cut(s)	994
TseI	GCWGC	7 cut(s)	50, 608, 611, 716, 971, 1277, 1320
Tsp45I	GTSAC	1 cut(s)	994
TspDTI	ATGAA	4 cut(s)	844, 859, 1133, 1181
TspRI	CASTG	1 cut(s)	1375
VpaK11BI	GGWCC	2 cut(s)	170, 587
XapI	RAATTY	1 cut(s)	1062
XceI	RCATGY	2 cut(s)	315, 949
XmiI	GTMKAC	1 cut(s)	791
XspI	CTAG	5 cut(s)	284, 398, 875, 999, 1343
Zsp2I	ATGCAT	1 cut(s)	1228

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.