Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0007336

Physical Location & Seq

Genomic Coordinates Forward (+)

8016096 .. 8021496

Gene Structure 5401 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g14210.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2181 bp

ATGGATGAAGATGGAGATATGGATTTGTTTTTCTCCAATTGGAACAACAAGAATCCGACTGATTGCCTATTTATTGTATCTTGCCTTGTTGCTGCCCTAGTCGGGATTTTGACTATAGCCTATTCTGCCTTCCAATGGAGGAGAAATATCAATCTAAGTTGGATGAAAGCCATAGCCAGGTCAAAGAAAAATCCAAAAGCACGTCACAAAGTTCCTGTAGCTCCTCATACTTGGGTTTTAGAATCTGTATCTCGTGGAAAGAACTTTAATTGCTGTGTTTGTTTCAATACCACTGGCCCTTCTCAAACCCTCGGGCCTCTGTCAGCTTCAGACAGTTTTCTGCACCGTTGCAGTATTTGTGATGCAGCGGCTCATCTAGGATGCTCTTCTAGTGCACAAAAGGACTGCAAGTGTGTATCCATGATTGGTTATGAGCGTGTAATGCACCAATGGGCTGTCCGTTGGACTGAGGTTTCAGACCAACCCGATGAGACTTCTTTCTGCAGCTATTGTGAAGAGCCCTGTAGTGGTTCCTTTCTTAGCGGATCCCCAATATGGTGCTGCCTGTGGTGTCAACGACTGGTACATGTTGATTGCCACAGTAGCATGTCCAATGAAACTGGTGATGTGTGTGATTTAGGTCCGTTCAGAAGGTTGATTCTATCACCCCTTCATGTTAAGGAATTGAACCAGACTCCGTCAGGTGGATTTTTGAGCTCAATTACTCATGGAGCCAATGAGATAGCATCTACAGTGCGTGCAAGTATTAGAAGTCAGAGCAAGAAGTACAAGCATGGAAATACAATCTCTACTGGGACTGGAAATAGTGGCAGTACTGGGGATATGTCTACCGAAAGCACAGCTGATACTAATCAAGCTGTTAATGGTTCTCATGAAATGGAGGAAAATTGCAACGGTGTTGTGGATGTGGATTTGCAGCACCGAGATACTGATGTAGATAACAAATTGGATTCTAAGCCCAGTATCCAAAAGAGCTCATCTATGAATAAGAAGGAAGAGTCTCTACGGATGAAACAAAGATATGAGTTAATTGATTTGTCGTCTGATGCAAGACCCTTGTTGGTTTTCATCAACAAGAAGAGTGGAGCTCAGCGTGGGAATTCCCTGAGACAACGTTTGAACATTCTTCTCAACCCCATCCAGGTATTTGAACTGAGTTCAGCACAGGGGCCAGAGGTGGGTCTGTACTTATTCAGAAAGGTGCCACACTTCAGAGTTCTTGTTTGTGGGGGAGATGGTACAGTTGGCTGGGTTCTGGATGCCATCGAGAAGCAAAATTTTGTCTCTCCTCCTCCAGTTGCTATTCTTCCTGCTGGAACAGGGAATGATCTGGCTAGAGTTTTATCGTGGGGAGGTGGTTTGGGTTCAGTGGAGAGACAGGGAGGCCTTTGCACTGTTTTGCACCACATAGAGCATGCAGCTGTAACCATTCTTGACCGGTGGAAAGTAGCAATTTTAAGTCAACAGGGAAAGCAACTTCAACCGCCAAAATTTATGAACAATTACCTTGGAATCGGATGTGATGCTAAGGTTGCTCTGGACATTCATAATCTTCGTGAGGAGAACCCAGAGAAGTTCTATAATCAGTTCATGAATAAAGTCCTTTATGCAAGAGAAGGGGCCAAGGGTATAATGGATAGAACATTTGTAGACTTTCCTTGGCAAGTTCGGGTTGAAGTAGATGGTGTTGAGATAGAGGTCCCTGAGGATGCAGAAGGTGTACTTGTTGCTAATATTGGAAGCTACATGGGTGGCGTAGATTTGTGGCAAAATGAAGATGAAACGTATGATAATTTTGATCCGCAATCCATGCATGACAAAATTCTTGAGGTTGTGAGCATATCTGGAACATGGCACCTTGGGAAACTACAGGTTGGGCTTTCTCGTGCTCGCAGACTTGCGCAGGGCCAGTCAATTAGGATACAGCTTTTTGCGGCATTTCCTGTTCAGATTGATGGGGAACCTTGGTTTCAGCAACCTTGTACATTATCGATATCTCATCATGGCCAGGCATTCATGTTGAAGAGGGCTGCTGAGGAGCCACTTGGTCATGCAGCTGCCATTATCACCGATGTCCTTGAGAATGCTGAAACAACCCATGTAATCAACGCTTCACAGAAGCGAGCTCTTCTTCAAGAAATGGCTCTCAAACTGACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

C1_1 DAGK_acc DAGK_cat

Protein Analysis

727

Amino Acids

80.22

Weight (kDa)

6.16

Isoelectric Point (pI)

47.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C1_1	PF00130	149 - 208	4.1e-09	Phorbol esters/diacylglycerol binding domain (C1 domain)
DAGK_cat	PF00781	359 - 459	2.1e-25	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	505 - 636	4.6e-45	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007336)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07920 AT5G07920 AT5G07920
fragaria_vesca	FvH4_5g14210 FvH4_5g14210 FvH4_5g14210 FvH4_5g14210 FvH4_5g14210 FvH4_5g14210
malus_domestica	MD14G1209800.v1.1
prunus_persica	Prupe.5G203000_v2.0.a1 Prupe.5G221300_v2.0.a1
pyrus_communis	pycom06g17690 pycom14g17330
rosa_chinensis	RchiOBHm_Chr7g0182721
rosa_laevigata	RLG00000005106
rosa_multiflora	Rmu_sc0000014.1_g000036
rosa_roxburghii	Rroxscaffold_3G00271040
rosa_rugosa	Rorug06G0449500 Rorug06G0449600
rosa_samantha	Rh7AG053300 Rh7BG052900 Rh7CG054500 Rh7DG052700
rosa_wichuraiana	Rw7G004340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1923
AccB1I	GGYRCC	2 cut(s)	1222, 1875
AccI	GTMKAC	2 cut(s)	848, 1671
AciI	CCGC	5 cut(s)	368, 543, 1505, 1823, 1955
AclI	AACGTT	1 cut(s)	1135
AclWI	GGATC	3 cut(s)	540, 553, 1814
AcoI	YGGCCR	1 cut(s)	2026
AcsI	RAATTY	4 cut(s)	1120, 1297, 1511, 1842
AcuI	CTGAAG	2 cut(s)	312, 1216
AfaI	GTAC	7 cut(s)	585, 788, 835, 1208, 1261, 1743, 2005
AfiI	CCNNNNNNNGG	8 cut(s)	102, 135, 177, 527, 555, 704, 1162, 1199
AflIII	ACRYGT	1 cut(s)	586
AgeI	ACCGGT	1 cut(s)	1458
AgsI	TTSAA	8 cut(s)	286, 688, 1141, 1172, 1502, 1697, 2044, 2156
AjiI	CACGTC	1 cut(s)	203
AjnI	CCWGG	3 cut(s)	176, 1161, 2028
Alw21I	GWGCWC	6 cut(s)	397, 719, 998, 1111, 1912, 2149
Alw26I	GTCTC	5 cut(s)	485, 1026, 1123, 1309, 1390
Alw44I	GTGCAC	1 cut(s)	393
AlwI	GGATC	3 cut(s)	540, 553, 1814
Ama87I	CYCGRG	1 cut(s)	311
AoxI	GGCC	7 cut(s)	295, 314, 1190, 1405, 1641, 1927, 2026
ApaLI	GTGCAC	1 cut(s)	393
ApeKI	GCWGC	9 cut(s)	92, 365, 504, 561, 937, 1439, 2051, 2075, 2078
ApoI	RAATTY	4 cut(s)	1120, 1297, 1511, 1842
AsiGI	ACCGGT	1 cut(s)	1458
AspLEI	GCGC	1 cut(s)	1924
AspS9I	GGNCC	7 cut(s)	296, 314, 641, 1190, 1641, 1720, 1927
AsuHPI	GGTGA	3 cut(s)	635, 657, 2080
AvaI	CYCGRG	1 cut(s)	311
AvaII	GGWCC	2 cut(s)	641, 1720
AxyI	CCTNAGG	1 cut(s)	1725
BaeGI	GKGCMC	1 cut(s)	397
BaeI	ACNNNNGTAYC	2 cut(s)	939, 972
BalI	TGGCCA	1 cut(s)	2028
BamHI	GGATCC	1 cut(s)	545
BanI	GGYRCC	2 cut(s)	1222, 1875
BanII	GRGCYC	5 cut(s)	522, 719, 998, 1111, 2149
BarI	GAAGNNNNNNTAC	2 cut(s)	1008, 1040
BauI	CACGAG	2 cut(s)	252, 1905
Bbv12I	GWGCWC	6 cut(s)	397, 719, 998, 1111, 1912, 2149
BbvCI	CCTCAGC	1 cut(s)	2055
BbvI	GCAGC	9 cut(s)	79, 377, 516, 548, 949, 1451, 2038, 2065, 2087
BccI	CCATC	6 cut(s)	5, 1166, 1250, 1292, 1697, 1970
BciT130I	CCWGG	3 cut(s)	178, 1163, 2030
BciVI	GTATCC	3 cut(s)	427, 995, 1935
BcoDI	GTCTC	5 cut(s)	485, 1026, 1123, 1309, 1390
BfaI	CTAG	4 cut(s)	98, 377, 390, 1356
BfmI	CTRYAG	6 cut(s)	114, 216, 502, 523, 750, 1889
BfuI	GTATCC	3 cut(s)	427, 995, 1935
BlpI	GCTNAGC	1 cut(s)	1110
BmcAI	AGTACT	1 cut(s)	835
Bme1390I	CCNGG	3 cut(s)	178, 1163, 2030
Bme18I	GGWCC	2 cut(s)	641, 1720
BmeT110I	CYCGRG	1 cut(s)	311
BmgBI	CACGTC	1 cut(s)	203
BmgT120I	GGNCC	7 cut(s)	296, 314, 641, 1190, 1641, 1720, 1927
BmrFI	CCNGG	3 cut(s)	178, 1163, 2030
BmrI	ACTGGG	3 cut(s)	822, 846, 975
BmsI	GCATC	7 cut(s)	352, 371, 755, 1057, 1270, 1534, 1720
BmuI	ACTGGG	3 cut(s)	822, 846, 975
BplI	GAGNNNNNCTC	2 cut(s)	1093, 1125
BpmI	CTGGAG	1 cut(s)	1299
Bpu10I	CCTNAGC	2 cut(s)	1548, 2055
Bpu1102I	GCTNAGC	1 cut(s)	1110
BpuEI	CTTGAG	2 cut(s)	1868, 2120
Bsa29I	ATCGAT	1 cut(s)	2012
BsaBI	GATNNNNATC	1 cut(s)	870
BsaJI	CCNNGG	6 cut(s)	310, 1528, 1644, 1679, 1879, 1985
BsaWI	WCCGGW	1 cut(s)	1458
Bsc4I	CCNNNNNNNGG	8 cut(s)	102, 135, 177, 527, 555, 704, 1162, 1199
Bse118I	RCCGGY	1 cut(s)	1458
Bse1I	ACTGG	9 cut(s)	298, 585, 625, 817, 823, 841, 981, 1316, 1930
Bse21I	CCTNAGG	1 cut(s)	1725
Bse8I	GATNNNNATC	1 cut(s)	870
BseBI	CCWGG	3 cut(s)	178, 1163, 2030
BseCI	ATCGAT	1 cut(s)	2012
BseDI	CCNNGG	6 cut(s)	310, 1528, 1644, 1679, 1879, 1985
BseGI	GGATG	9 cut(s)	10, 168, 386, 931, 1035, 1158, 1285, 1544, 1735
BseJI	GATNNNNATC	1 cut(s)	870
BseLI	CCNNNNNNNGG	8 cut(s)	102, 135, 177, 527, 555, 704, 1162, 1199
BseMII	CTCAG	6 cut(s)	459, 1118, 1124, 1166, 1716, 2046
BseNI	ACTGG	9 cut(s)	298, 585, 625, 817, 823, 841, 981, 1316, 1930
BseRI	GAGGAG	6 cut(s)	154, 213, 1299, 1302, 1595, 2072
BseSI	GKGCMC	1 cut(s)	397
BseXI	GCAGC	9 cut(s)	79, 377, 516, 548, 949, 1451, 2038, 2065, 2087
BseYI	CCCAGC	1 cut(s)	1269
BsgI	GTGCAG	1 cut(s)	326
BshFI	GGCC	7 cut(s)	297, 316, 1192, 1407, 1643, 1929, 2028
BshNI	GGYRCC	2 cut(s)	1222, 1875
BshTI	ACCGGT	1 cut(s)	1458
BshVI	ATCGAT	1 cut(s)	2012
BsiHKAI	GWGCWC	6 cut(s)	397, 719, 998, 1111, 1912, 2149
BsiHKCI	CYCGRG	1 cut(s)	311
BsiSI	CCGG	1 cut(s)	1459
BslFI	GGGAC	2 cut(s)	829, 1706
BslI	CCNNNNNNNGG	8 cut(s)	102, 135, 177, 527, 555, 704, 1162, 1199
BsmAI	GTCTC	5 cut(s)	485, 1026, 1123, 1309, 1390
BsmFI	GGGAC	2 cut(s)	829, 1706
BsmI	GAATGC	2 cut(s)	2033, 2110
BsnI	GGCC	7 cut(s)	297, 316, 1192, 1407, 1643, 1929, 2028
BsoBI	CYCGRG	1 cut(s)	311
Bsp1286I	GDGCHC	7 cut(s)	397, 522, 719, 998, 1111, 1912, 2149
Bsp1407I	TGTACA	1 cut(s)	2003
Bsp143I	GATC	3 cut(s)	545, 1348, 1819
Bsp1720I	GCTNAGC	1 cut(s)	1110
BspACI	CCGC	5 cut(s)	368, 543, 1505, 1823, 1955
BspANI	GGCC	7 cut(s)	297, 316, 1192, 1407, 1643, 1929, 2028
BspCNI	CTCAG	6 cut(s)	460, 1119, 1123, 1167, 1717, 2047
BspDI	ATCGAT	1 cut(s)	2012
BspHI	TCATGA	2 cut(s)	892, 1611
BspMAI	CTGCAG	1 cut(s)	506
BspPI	GGATC	3 cut(s)	540, 553, 1814
BspQI	GCTCTTC	3 cut(s)	391, 510, 2154
BspT107I	GGYRCC	2 cut(s)	1222, 1875
BsrFI	RCCGGY	1 cut(s)	1458
BsrGI	TGTACA	1 cut(s)	2003
BsrI	ACTGG	9 cut(s)	298, 585, 625, 817, 823, 841, 981, 1316, 1930
BssAI	RCCGGY	1 cut(s)	1458
BssECI	CCNNGG	6 cut(s)	310, 1528, 1644, 1679, 1879, 1985
BssMI	GATC	3 cut(s)	545, 1348, 1819
BssSI	CACGAG	2 cut(s)	252, 1905
BssT1I	CCWWGG	5 cut(s)	1528, 1644, 1679, 1879, 1985
Bst2BI	CACGAG	2 cut(s)	252, 1905
Bst2UI	CCWGG	3 cut(s)	178, 1163, 2030
Bst4CI	ACNGT	7 cut(s)	335, 347, 602, 754, 917, 1264, 1417
Bst6I	CTCTTC	6 cut(s)	391, 510, 1011, 1094, 2039, 2154
BstAPI	GCANNNNNTGC	1 cut(s)	1831
BstAUI	TGTACA	1 cut(s)	2003
BstC8I	GCNNGC	4 cut(s)	759, 1437, 1912, 2145
BstF5I	GGATG	9 cut(s)	10, 168, 386, 931, 1035, 1158, 1285, 1544, 1735
BstHHI	GCGC	1 cut(s)	1924
BstKTI	GATC	3 cut(s)	548, 1351, 1822
BstMAI	GTCTC	5 cut(s)	485, 1026, 1123, 1309, 1390
BstMBI	GATC	3 cut(s)	545, 1348, 1819
BstMWI	GCNNNNNNNGC	5 cut(s)	125, 442, 603, 1553, 1831
BstNI	CCWGG	3 cut(s)	178, 1163, 2030
BstNSI	RCATGY	3 cut(s)	590, 610, 1439
BstSCI	CCNGG	3 cut(s)	176, 1161, 2028
BstSFI	CTRYAG	6 cut(s)	114, 216, 502, 523, 750, 1889
BstSLI	GKGCMC	1 cut(s)	397
BstV1I	GCAGC	9 cut(s)	79, 377, 516, 548, 949, 1451, 2038, 2065, 2087
BstX2I	RGATCY	1 cut(s)	545
BstYI	RGATCY	1 cut(s)	545
Bsu15I	ATCGAT	1 cut(s)	2012
Bsu36I	CCTNAGG	1 cut(s)	1725
BsuI	GTATCC	3 cut(s)	427, 995, 1935
BsuRI	GGCC	7 cut(s)	297, 316, 1192, 1407, 1643, 1929, 2028
BsuTUI	ATCGAT	1 cut(s)	2012
BtrI	CACGTC	1 cut(s)	203
BtsCI	GGATG	9 cut(s)	10, 168, 386, 931, 1035, 1158, 1285, 1544, 1735
BtsIMutI	CAGTG	4 cut(s)	291, 759, 1395, 1413
Cac8I	GCNNGC	4 cut(s)	759, 1437, 1912, 2145
CciI	TCATGA	2 cut(s)	892, 1611
CfoI	GCGC	1 cut(s)	1924
Cfr10I	RCCGGY	1 cut(s)	1458
Cfr13I	GGNCC	7 cut(s)	296, 314, 641, 1190, 1641, 1720, 1927
ClaI	ATCGAT	1 cut(s)	2012
Csp6I	GTAC	7 cut(s)	584, 787, 834, 1207, 1260, 1742, 2004
CspAI	ACCGGT	1 cut(s)	1458
CspCI	CAANNNNNGTGG	2 cut(s)	1084, 1119
CviQI	GTAC	7 cut(s)	584, 787, 834, 1207, 1260, 1742, 2004
DpnI	GATC	3 cut(s)	547, 1350, 1821
DpnII	GATC	3 cut(s)	545, 1348, 1819
EaeI	YGGCCR	1 cut(s)	2026
Eam1104I	CTCTTC	6 cut(s)	391, 510, 1011, 1094, 2039, 2154
EarI	CTCTTC	6 cut(s)	391, 510, 1011, 1094, 2039, 2154
Ecl136II	GAGCTC	4 cut(s)	717, 996, 1109, 2147
Eco130I	CCWWGG	5 cut(s)	1528, 1644, 1679, 1879, 1985
Eco147I	AGGCCT	1 cut(s)	1407
Eco24I	GRGCYC	5 cut(s)	522, 719, 998, 1111, 2149
Eco32I	GATATC	1 cut(s)	2016
Eco47I	GGWCC	2 cut(s)	641, 1720
Eco53kI	GAGCTC	4 cut(s)	717, 996, 1109, 2147
Eco57I	CTGAAG	2 cut(s)	312, 1216
Eco81I	CCTNAGG	1 cut(s)	1725
Eco88I	CYCGRG	1 cut(s)	311
EcoICRI	GAGCTC	4 cut(s)	717, 996, 1109, 2147
EcoO109I	RGGNCCY	1 cut(s)	1720
EcoRI	GAATTC	1 cut(s)	1120
EcoRII	CCWGG	3 cut(s)	176, 1161, 2028
EcoRV	GATATC	1 cut(s)	2016
EcoT14I	CCWWGG	5 cut(s)	1528, 1644, 1679, 1879, 1985
EcoT22I	ATGCAT	1 cut(s)	1836
EcoT38I	GRGCYC	5 cut(s)	522, 719, 998, 1111, 2149
ErhI	CCWWGG	5 cut(s)	1528, 1644, 1679, 1879, 1985
FalI	AAGNNNNNCTT	2 cut(s)	1728, 1760
FaqI	GGGAC	2 cut(s)	829, 1706
FblI	GTMKAC	2 cut(s)	848, 1671
FokI	GGATG	9 cut(s)	17, 175, 393, 938, 1042, 1145, 1292, 1551, 1742
FriOI	GRGCYC	5 cut(s)	522, 719, 998, 1111, 2149
FspBI	CTAG	4 cut(s)	98, 377, 390, 1356
FspI	TGCGCA	1 cut(s)	1923
GlaI	GCGC	1 cut(s)	1923
GsaI	CCCAGC	1 cut(s)	1273
GsuI	CTGGAG	1 cut(s)	1299
HaeIII	GGCC	7 cut(s)	297, 316, 1192, 1407, 1643, 1929, 2028
HapII	CCGG	1 cut(s)	1459
HhaI	GCGC	1 cut(s)	1924
Hin6I	GCGC	1 cut(s)	1922
HinP1I	GCGC	1 cut(s)	1922
HincII	GTYRAC	2 cut(s)	575, 1484
HindII	GTYRAC	2 cut(s)	575, 1484
HinfI	GANTC	7 cut(s)	52, 242, 658, 694, 971, 1019, 1533
HpaII	CCGG	1 cut(s)	1459
HphI	GGTGA	3 cut(s)	635, 657, 2080
Hpy166II	GTNNAC	6 cut(s)	395, 575, 849, 1484, 1672, 1742
Hpy8I	GTNNAC	6 cut(s)	395, 575, 849, 1484, 1672, 1742
HpyAV	CCTTC	7 cut(s)	139, 309, 645, 680, 1006, 1631, 1730
HpyCH4III	ACNGT	7 cut(s)	335, 347, 602, 754, 917, 1264, 1417
HpyCH4IV	ACGT	3 cut(s)	202, 1135, 1805
HpyF10VI	GCNNNNNNNGC	5 cut(s)	125, 442, 603, 1553, 1831
HpySE526I	ACGT	3 cut(s)	202, 1135, 1805
HspAI	GCGC	1 cut(s)	1922
Kzo9I	GATC	3 cut(s)	545, 1348, 1819
LguI	GCTCTTC	3 cut(s)	391, 510, 2154
LmnI	GCTCC	4 cut(s)	226, 731, 1106, 2059
Lsp1109I	GCAGC	9 cut(s)	79, 377, 516, 548, 949, 1451, 2038, 2065, 2087
LweI	GCATC	7 cut(s)	352, 371, 755, 1057, 1270, 1534, 1720
MaeI	CTAG	4 cut(s)	98, 377, 390, 1356
MaeII	ACGT	3 cut(s)	202, 1135, 1805
MaeIII	GTNAC	2 cut(s)	203, 1444
MalI	GATC	3 cut(s)	547, 1350, 1821
MboI	GATC	3 cut(s)	545, 1348, 1819
MfeI	CAATTG	1 cut(s)	37
MflI	RGATCY	1 cut(s)	545
MhlI	GDGCHC	7 cut(s)	397, 522, 719, 998, 1111, 1912, 2149
MlsI	TGGCCA	1 cut(s)	2028
MluNI	TGGCCA	1 cut(s)	2028
MlyI	GAGTC	2 cut(s)	688, 1028
MmeI	TCCRAC	3 cut(s)	80, 140, 443
Mox20I	TGGCCA	1 cut(s)	2028
Mph1103I	ATGCAT	1 cut(s)	1836
MscI	TGGCCA	1 cut(s)	2028
MseI	TTAA	5 cut(s)	267, 678, 882, 1049, 1478
Msp20I	TGGCCA	1 cut(s)	2028
MspA1I	CMGCKG	4 cut(s)	368, 863, 1442, 2078
MspI	CCGG	1 cut(s)	1459
MspR9I	CCNGG	3 cut(s)	178, 1163, 2030
MunI	CAATTG	1 cut(s)	37
Mva1269I	GAATGC	2 cut(s)	2033, 2110
MvaI	CCWGG	3 cut(s)	178, 1163, 2030
MwoI	GCNNNNNNNGC	5 cut(s)	125, 442, 603, 1553, 1831
NdeII	GATC	3 cut(s)	545, 1348, 1819
NmuCI	GTSAC	1 cut(s)	203
NsbI	TGCGCA	1 cut(s)	1923
NsiI	ATGCAT	1 cut(s)	1836
NspI	RCATGY	3 cut(s)	590, 610, 1439
PaeI	GCATGC	1 cut(s)	1439
PagI	TCATGA	2 cut(s)	892, 1611
PceI	AGGCCT	1 cut(s)	1407
PciI	ACATGT	1 cut(s)	586
PciSI	GCTCTTC	3 cut(s)	391, 510, 2154
PctI	GAATGC	2 cut(s)	2033, 2110
PfeI	GAWTC	5 cut(s)	52, 242, 658, 971, 1533
PflFI	GACNNNGTC	1 cut(s)	697
PinAI	ACCGGT	1 cut(s)	1458
PleI	GAGTC	2 cut(s)	688, 1027
PpsI	GAGTC	2 cut(s)	688, 1027
PpuMI	RGGWCCY	1 cut(s)	1720
PscI	ACATGT	1 cut(s)	586
Psp124BI	GAGCTC	4 cut(s)	719, 998, 1111, 2149
Psp1406I	AACGTT	1 cut(s)	1135
Psp5II	RGGWCCY	1 cut(s)	1720
Psp6I	CCWGG	3 cut(s)	176, 1161, 2028
PspFI	CCCAGC	1 cut(s)	1269
PspGI	CCWGG	3 cut(s)	176, 1161, 2028
PspPI	GGNCC	7 cut(s)	296, 314, 641, 1190, 1641, 1720, 1927
PspPPI	RGGWCCY	1 cut(s)	1720
PstI	CTGCAG	1 cut(s)	506
PsuI	RGATCY	1 cut(s)	545
PsyI	GACNNNGTC	1 cut(s)	697
PvuII	CAGCTG	3 cut(s)	863, 1442, 2078
RsaI	GTAC	7 cut(s)	585, 788, 835, 1208, 1261, 1743, 2005
RsaNI	GTAC	7 cut(s)	584, 787, 834, 1207, 1260, 1742, 2004
SacI	GAGCTC	4 cut(s)	719, 998, 1111, 2149
SapI	GCTCTTC	3 cut(s)	391, 510, 2154
SaqAI	TTAA	5 cut(s)	267, 678, 882, 1049, 1478
Sau3AI	GATC	3 cut(s)	545, 1348, 1819
Sau96I	GGNCC	7 cut(s)	296, 314, 641, 1190, 1641, 1720, 1927
ScaI	AGTACT	1 cut(s)	835
SchI	GAGTC	2 cut(s)	688, 1028
ScrFI	CCNGG	3 cut(s)	178, 1163, 2030
SduI	GDGCHC	7 cut(s)	397, 522, 719, 998, 1111, 1912, 2149
SfaNI	GCATC	7 cut(s)	352, 371, 755, 1057, 1270, 1534, 1720
SfcI	CTRYAG	6 cut(s)	114, 216, 502, 523, 750, 1889
SinI	GGWCC	2 cut(s)	641, 1720
SmlI	CTYRAG	2 cut(s)	1847, 2099
SmoI	CTYRAG	2 cut(s)	1847, 2099
SphI	GCATGC	1 cut(s)	1439
SseBI	AGGCCT	1 cut(s)	1407
SsiI	CCGC	5 cut(s)	368, 543, 1505, 1823, 1955
SspI	AATATT	1 cut(s)	1756
SspMI	CTAG	4 cut(s)	98, 377, 390, 1356
SstI	GAGCTC	4 cut(s)	719, 998, 1111, 2149
StuI	AGGCCT	1 cut(s)	1407
StyD4I	CCNGG	3 cut(s)	176, 1161, 2028
StyI	CCWWGG	5 cut(s)	1528, 1644, 1679, 1879, 1985
TaaI	ACNGT	7 cut(s)	335, 347, 602, 754, 917, 1264, 1417
TaiI	ACGT	3 cut(s)	205, 1138, 1808
TaqI	TCGA	2 cut(s)	1287, 2012
TatI	WGTACW	5 cut(s)	786, 833, 1206, 1741, 2003
TauI	GCSGC	2 cut(s)	371, 1958
TfiI	GAWTC	5 cut(s)	52, 242, 658, 971, 1533
Tru1I	TTAA	5 cut(s)	267, 678, 882, 1049, 1478
Tru9I	TTAA	5 cut(s)	267, 678, 882, 1049, 1478
TscAI	CASTG	4 cut(s)	298, 759, 1395, 1420
TseFI	GTSAC	1 cut(s)	203
TseI	GCWGC	9 cut(s)	92, 365, 504, 561, 937, 1439, 2051, 2075, 2078
Tsp45I	GTSAC	1 cut(s)	203
TspGWI	ACGGA	4 cut(s)	449, 633, 687, 1042
TspRI	CASTG	4 cut(s)	298, 759, 1395, 1420
Tth111I	GACNNNGTC	1 cut(s)	697
VneI	GTGCAC	1 cut(s)	393
VpaK11BI	GGWCC	2 cut(s)	641, 1720
XapI	RAATTY	4 cut(s)	1120, 1297, 1511, 1842
XceI	RCATGY	3 cut(s)	590, 610, 1439
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1651
XmiI	GTMKAC	2 cut(s)	848, 1671
XspI	CTAG	4 cut(s)	98, 377, 390, 1356
ZrmI	AGTACT	1 cut(s)	835
Zsp2I	ATGCAT	1 cut(s)	1836

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.