Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0007336

Physical Location & Seq

Genomic Coordinates Forward (+)

63477326 .. 63482011

Gene Structure 4686 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00271040.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2061 bp

ATGGATGAGGATGGAGATATTGATATGTTTTTCTCCAATTGGAACAACAAGAATCCAACTGATCGCCTCTTTGTTGTCTCTTGCTTTGTTGCTGCCCTAGTTGGAATTCTGACTATAGCCTATTCTGCCTTCCAATGGAGGAGAAACATCAATCTAAGTTGGATGAAAGCCATAGCCAGGTCAAAGAAAAATCCAAAAGCGCGTCACAAAGTTCCCGTAGCTCCTCATACTTGGGTGTTAGAATCTGTATCTCGGGGAAAGAACTTTAATTGCTGTGTGTGTTTTAATACCACAGGCCCTTCTCAAACCCTCGGCCCTCTGGCAGCTTCAGACAGTTTTCTGCACCGTTGCAGTATTTGTGATGCAGCGGCTCATCTAAGTTGCTCTTCTAGTGCACAAAAGGACTGCAAGTGTGTATCTATGATTGGTTATGAGCGTGTAATGCACCAATGGGCTGTCCGTTGGACAGAGGTAACGGACCAACCCGATGAGACTTCTTTCTGTAGCTACTGTGAAGAGCCCTGTAGTGGTTCCTTTCTTAGTGGATCCCCAATATGGTGCTGCTTGTGGTGTCAACGACTGGTACATGTTGATTGCCACAGCAGCATGTCCAATGAAACAGGTGATGTATGTGATTTAGGTCCGTTCAGAAGGTTGATTCTATCTCCCCTTCATGTTAAGGAATTGAACCAGAATCCATCAGGTGGATTCTTGAGCTCAATTACTCATGGAGCCAATGAGATAGCATCTACAGTACGTGCAAGTATTAGGAGTCAGAGCAAGAAGTACAAGCATGGAAATGCAATCTCTACTGGGACGGGAAATAGTGGTAGTACGGGTGATATGTCTACTGAAAGCACAGCTGATACCAATCAAGCTGTTAATGTTTCTCATGAAATGGAGGAAAATTGTAATGGTACCGTGGATGTGGATTTGCAGCACCAAGATGCTGATGTAGATAACAAATTGGATTCTAAGCCCAGTTTCCAAAAGAGCTCGTCTATGACTAAGAAGGATGAGTCTTTACGGATGAAACAAAGATATGAGTTGACTGATTTGTCGTCAGATGCAAGACCGTTGTTGGTTTTCATCAACAAGAAGAGTGGAGCTCAGCGTGGGAATTCACTGAGGCAACGTTTGAATATTCTTCTCAACCCCGTCCAGGTATTTGAACTGAGTTCAGCACAGGGACCAGAGGTGGGTCTGTACTTATTCAGAAAGGTGCCACACTTCAGAGTTCTTGTTTGCGGGGGAGATGGTACTGTTGGTTGGGTTCTGGATGCCATTGAGAAGCAAAATTTCGTCTCTCCTCCTCCAGTTGCTATTCTTCCTGCCGGAACTGGGAATGATCTGGCAAGAGTTTTATCGTGGGGAGGTGGTTTGGGTGCAGTGGAGAGACAAGGAGGCCTTTGCACTGTTCTGCACCACATAGAGCATGCCGCTGTAACCATTCTTGACCGGTGGAAAGTGGCAATTTTAAATCAACAGGGAAAGCAACTTCAACCGCCAAAATTTATGAACAATTATCTTGGAATCGGATGTGATGCCAAGGTTGCTCTGGACATTCATAATCTTCGTGAGGAGAACCCGGAGAAGTTTTATAATCAGGATGCAGAAGGTGTACTTGTTGCTAATATTGGAAGCTACATGGGTGGCGTAGATTTGTGGCAAAATGAAGATGAAACATATGATAATTTTGATCCGCAATCCATGCATGACAAAATACTTGAGGTTGTGAGCATATCTGGAACATGGCACCTTGGGAAACTACAGGTTGGGCTTTCTCGTGCTCGAAGACTTGCGCAGGGCCAGTCAATGAAGATACAGCTTTGTGCGGCATTTCCTGTTCAGATTGATGGGGAGCCTTGGTTTCAGCAACCTTGTACATTGTCTATATCCCATCATGGCCAGGCATTCATGTTGAAGAGGGCTGCTGAGGAGCCACTTGGTCATGCAGCTGCCATTATCACCGATGTCCTTGAGAGTGCTGAAACGACCCATGTAATCAACGCTTCACAGAAGCGGGCTCTTCTTCAAGAAATGGCACTCAGACTGACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

C1_1 DAGK_acc DAGK_cat

Protein Analysis

686

Amino Acids

75.58

Weight (kDa)

6.37

Isoelectric Point (pI)

48.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C1_1	PF00130	149 - 208	4.6e-09	Phorbol esters/diacylglycerol binding domain (C1 domain)
DAGK_cat	PF00781	359 - 459	1.5e-25	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	505 - 536	5.5e-09	Diacylglycerol kinase accessory domain
DAGK_acc	PF00609	536 - 622	6.3e-27	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007336)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07920 AT5G07920 AT5G07920
fragaria_vesca	FvH4_5g14210 FvH4_5g14210 FvH4_5g14210 FvH4_5g14210 FvH4_5g14210 FvH4_5g14210
malus_domestica	MD14G1209800.v1.1
prunus_persica	Prupe.5G203000_v2.0.a1 Prupe.5G221300_v2.0.a1
pyrus_communis	pycom06g17690 pycom14g17330
rosa_chinensis	RchiOBHm_Chr7g0182721
rosa_laevigata	RLG00000005106
rosa_multiflora	Rmu_sc0000014.1_g000036
rosa_roxburghii	Rroxscaffold_3G00271040
rosa_rugosa	Rorug06G0449500 Rorug06G0449600
rosa_samantha	Rh7AG053300 Rh7BG052900 Rh7CG054500 Rh7DG052700
rosa_wichuraiana	Rw7G004340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1602
AasI	GACNNNNNNGTC	1 cut(s)	1057
Acc16I	TGCGCA	1 cut(s)	1803
Acc65I	GGTACC	1 cut(s)	917
AccB1I	GGYRCC	3 cut(s)	917, 1222, 1755
AccB7I	CCANNNNNTGG	1 cut(s)	704
AccI	GTMKAC	1 cut(s)	848
AccII	CGCG	1 cut(s)	202
AciI	CCGC	7 cut(s)	368, 1248, 1440, 1505, 1703, 1835, 2023
AclI	AACGTT	1 cut(s)	1135
AclWI	GGATC	3 cut(s)	540, 553, 1694
AcoI	YGGCCR	1 cut(s)	1906
AcsI	RAATTY	4 cut(s)	105, 1120, 1297, 1511
AcuI	CTGAAG	2 cut(s)	312, 1216
AfaI	GTAC	9 cut(s)	585, 756, 788, 835, 919, 1208, 1261, 1623, 1885
AfiI	CCNNNNNNNGG	8 cut(s)	135, 177, 527, 555, 704, 1162, 1199, 1341
AflIII	ACRYGT	1 cut(s)	586
AgeI	ACCGGT	1 cut(s)	1458
AgsI	TTSAA	6 cut(s)	688, 1141, 1172, 1502, 1924, 2036
AjnI	CCWGG	3 cut(s)	176, 1161, 1908
Alw21I	GWGCWC	5 cut(s)	397, 719, 998, 1111, 1792
Alw26I	GTCTC	4 cut(s)	82, 485, 1309, 1390
Alw44I	GTGCAC	1 cut(s)	393
AlwI	GGATC	3 cut(s)	540, 553, 1694
Ama87I	CYCGRG	1 cut(s)	252
AoxI	GGCC	5 cut(s)	295, 313, 1405, 1807, 1906
ApaLI	GTGCAC	1 cut(s)	393
ApeKI	GCWGC	9 cut(s)	92, 323, 365, 561, 603, 937, 1931, 1955, 1958
ApoI	RAATTY	4 cut(s)	105, 1120, 1297, 1511
AsiGI	ACCGGT	1 cut(s)	1458
Asp718I	GGTACC	1 cut(s)	917
AspLEI	GCGC	2 cut(s)	202, 1804
AspS9I	GGNCC	6 cut(s)	296, 314, 478, 641, 1190, 1807
AsuC2I	CCSGG	1 cut(s)	1589
AsuHPI	GGTGA	3 cut(s)	635, 851, 1960
AvaI	CYCGRG	1 cut(s)	252
AvaII	GGWCC	3 cut(s)	478, 641, 1190
BaeGI	GKGCMC	1 cut(s)	397
BalI	TGGCCA	1 cut(s)	1908
BamHI	GGATCC	1 cut(s)	545
BanI	GGYRCC	3 cut(s)	917, 1222, 1755
BanII	GRGCYC	5 cut(s)	522, 719, 998, 1111, 2029
BauI	CACGAG	1 cut(s)	1785
BbsI	GAAGAC	1 cut(s)	1801
Bbv12I	GWGCWC	5 cut(s)	397, 719, 998, 1111, 1792
BbvCI	CCTCAGC	1 cut(s)	1935
BbvI	GCAGC	9 cut(s)	79, 335, 377, 548, 615, 949, 1918, 1945, 1967
BccI	CCATC	5 cut(s)	5, 706, 1250, 1850, 1908
BciT130I	CCWGG	3 cut(s)	178, 1163, 1910
BcnI	CCSGG	1 cut(s)	1589
BcoDI	GTCTC	4 cut(s)	82, 485, 1309, 1390
BfaI	CTAG	2 cut(s)	98, 390
BfmI	CTRYAG	5 cut(s)	114, 502, 523, 750, 1769
BlpI	GCTNAGC	1 cut(s)	1110
Bme1390I	CCNGG	4 cut(s)	178, 1163, 1589, 1910
Bme18I	GGWCC	3 cut(s)	478, 641, 1190
BmeT110I	CYCGRG	1 cut(s)	252
BmgT120I	GGNCC	6 cut(s)	296, 314, 478, 641, 1190, 1807
BmiI	GGNNCC	9 cut(s)	532, 547, 733, 919, 1191, 1224, 1757, 1863, 1941
BmrFI	CCNGG	4 cut(s)	178, 1163, 1589, 1910
BmrI	ACTGGG	3 cut(s)	822, 975, 1350
BmsI	GCATC	7 cut(s)	352, 755, 937, 1057, 1270, 1534, 1600
BmuI	ACTGGG	3 cut(s)	822, 975, 1350
BpiI	GAAGAC	1 cut(s)	1801
BplI	GAGNNNNNCTC	2 cut(s)	1093, 1125
BpmI	CTGGAG	1 cut(s)	1299
Bpu10I	CCTNAGC	1 cut(s)	1935
Bpu1102I	GCTNAGC	1 cut(s)	1110
BpuEI	CTTGAG	3 cut(s)	733, 1748, 2000
BpuMI	CCSGG	1 cut(s)	1589
BsaAI	YACGTR	1 cut(s)	758
BsaBI	GATNNNNATC	1 cut(s)	870
BsaJI	CCNNGG	5 cut(s)	310, 921, 1548, 1759, 1865
BsaWI	WCCGGW	1 cut(s)	1458
BsaXI	ACNNNNNCTCC	2 cut(s)	1853, 1883
Bsc4I	CCNNNNNNNGG	8 cut(s)	135, 177, 527, 555, 704, 1162, 1199, 1341
Bse118I	RCCGGY	1 cut(s)	1458
Bse1I	ACTGG	6 cut(s)	585, 817, 981, 1316, 1345, 1810
Bse8I	GATNNNNATC	1 cut(s)	870
BseBI	CCWGG	3 cut(s)	178, 1163, 1910
BseDI	CCNNGG	5 cut(s)	310, 921, 1548, 1759, 1865
BseGI	GGATG	9 cut(s)	10, 16, 168, 931, 1021, 1035, 1285, 1544, 1615
BseJI	GATNNNNATC	1 cut(s)	870
BseLI	CCNNNNNNNGG	8 cut(s)	135, 177, 527, 555, 704, 1162, 1199, 1341
BseMII	CTCAG	4 cut(s)	1118, 1124, 1166, 1926
BseNI	ACTGG	6 cut(s)	585, 817, 981, 1316, 1345, 1810
BseRI	GAGGAG	6 cut(s)	154, 213, 1299, 1302, 1595, 1952
BseSI	GKGCMC	1 cut(s)	397
BseXI	GCAGC	9 cut(s)	79, 335, 377, 548, 615, 949, 1918, 1945, 1967
BsgI	GTGCAG	3 cut(s)	326, 1406, 1407
Bsh1236I	CGCG	1 cut(s)	202
BshFI	GGCC	5 cut(s)	297, 315, 1407, 1809, 1908
BshNI	GGYRCC	3 cut(s)	917, 1222, 1755
BshTI	ACCGGT	1 cut(s)	1458
BsiHKAI	GWGCWC	5 cut(s)	397, 719, 998, 1111, 1792
BsiHKCI	CYCGRG	1 cut(s)	252
BsiSI	CCGG	3 cut(s)	1335, 1459, 1589
BslFI	GGGAC	2 cut(s)	829, 1203
BslI	CCNNNNNNNGG	8 cut(s)	135, 177, 527, 555, 704, 1162, 1199, 1341
BsmAI	GTCTC	4 cut(s)	82, 485, 1309, 1390
BsmBI	CGTCTC	1 cut(s)	1309
BsmFI	GGGAC	2 cut(s)	829, 1203
BsmI	GAATGC	1 cut(s)	1913
BsnI	GGCC	5 cut(s)	297, 315, 1407, 1809, 1908
BsoBI	CYCGRG	1 cut(s)	252
Bsp1286I	GDGCHC	7 cut(s)	397, 522, 719, 998, 1111, 1792, 2029
Bsp1407I	TGTACA	1 cut(s)	1883
Bsp143I	GATC	4 cut(s)	61, 545, 1348, 1699
Bsp1720I	GCTNAGC	1 cut(s)	1110
BspACI	CCGC	7 cut(s)	368, 1248, 1440, 1505, 1703, 1835, 2023
BspANI	GGCC	5 cut(s)	297, 315, 1407, 1809, 1908
BspCNI	CTCAG	5 cut(s)	1119, 1123, 1167, 1927, 2061
BspFNI	CGCG	1 cut(s)	202
BspHI	TCATGA	1 cut(s)	892
BspLI	GGNNCC	9 cut(s)	532, 547, 733, 919, 1191, 1224, 1757, 1863, 1941
BspPI	GGATC	3 cut(s)	540, 553, 1694
BspQI	GCTCTTC	3 cut(s)	391, 510, 2034
BspT107I	GGYRCC	3 cut(s)	917, 1222, 1755
BsrFI	RCCGGY	1 cut(s)	1458
BsrGI	TGTACA	1 cut(s)	1883
BsrI	ACTGG	6 cut(s)	585, 817, 981, 1316, 1345, 1810
BssAI	RCCGGY	1 cut(s)	1458
BssECI	CCNNGG	5 cut(s)	310, 921, 1548, 1759, 1865
BssMI	GATC	4 cut(s)	61, 545, 1348, 1699
BssSI	CACGAG	1 cut(s)	1785
BssT1I	CCWWGG	3 cut(s)	1548, 1759, 1865
Bst2BI	CACGAG	1 cut(s)	1785
Bst2UI	CCWGG	3 cut(s)	178, 1163, 1910
Bst4CI	ACNGT	8 cut(s)	335, 347, 512, 754, 922, 1077, 1264, 1417
Bst6I	CTCTTC	5 cut(s)	391, 510, 1094, 1919, 2034
BstAPI	GCANNNNNTGC	1 cut(s)	1711
BstAUI	TGTACA	1 cut(s)	1883
BstBAI	YACGTR	1 cut(s)	758
BstC8I	GCNNGC	2 cut(s)	1437, 2025
BstDSI	CCRYGG	1 cut(s)	921
BstF5I	GGATG	9 cut(s)	10, 16, 168, 931, 1021, 1035, 1285, 1544, 1615
BstFNI	CGCG	1 cut(s)	202
BstHHI	GCGC	2 cut(s)	202, 1804
BstKTI	GATC	4 cut(s)	64, 548, 1351, 1702
BstMAI	GTCTC	4 cut(s)	82, 485, 1309, 1390
BstMBI	GATC	4 cut(s)	61, 545, 1348, 1699
BstMWI	GCNNNNNNNGC	5 cut(s)	125, 442, 603, 1553, 1711
BstNI	CCWGG	3 cut(s)	178, 1163, 1910
BstNSI	RCATGY	3 cut(s)	590, 610, 1439
BstSCI	CCNGG	4 cut(s)	176, 1161, 1587, 1908
BstSFI	CTRYAG	5 cut(s)	114, 502, 523, 750, 1769
BstSLI	GKGCMC	1 cut(s)	397
BstUI	CGCG	1 cut(s)	202
BstV1I	GCAGC	9 cut(s)	79, 335, 377, 548, 615, 949, 1918, 1945, 1967
BstV2I	GAAGAC	1 cut(s)	1801
BstX2I	RGATCY	1 cut(s)	545
BstYI	RGATCY	1 cut(s)	545
BsuRI	GGCC	5 cut(s)	297, 315, 1407, 1809, 1908
BtgI	CCRYGG	1 cut(s)	921
BtsCI	GGATG	9 cut(s)	10, 16, 168, 931, 1021, 1035, 1285, 1544, 1615
BtsI	GCAGTG	1 cut(s)	1395
BtsIMutI	CAGTG	3 cut(s)	1124, 1395, 1413
Cac8I	GCNNGC	2 cut(s)	1437, 2025
CciI	TCATGA	1 cut(s)	892
CfoI	GCGC	2 cut(s)	202, 1804
Cfr10I	RCCGGY	1 cut(s)	1458
Cfr13I	GGNCC	6 cut(s)	296, 314, 478, 641, 1190, 1807
CseI	GACGC	1 cut(s)	191
Csp6I	GTAC	9 cut(s)	584, 755, 787, 834, 918, 1207, 1260, 1622, 1884
CspAI	ACCGGT	1 cut(s)	1458
CspCI	CAANNNNNGTGG	2 cut(s)	1084, 1119
CviQI	GTAC	9 cut(s)	584, 755, 787, 834, 918, 1207, 1260, 1622, 1884
DpnI	GATC	4 cut(s)	63, 547, 1350, 1701
DpnII	GATC	4 cut(s)	61, 545, 1348, 1699
DraI	TTTAAA	1 cut(s)	1479
DrdI	GACNNNNNNGTC	1 cut(s)	1057
DseDI	GACNNNNNNGTC	1 cut(s)	1057
EaeI	YGGCCR	1 cut(s)	1906
Eam1104I	CTCTTC	5 cut(s)	391, 510, 1094, 1919, 2034
EarI	CTCTTC	5 cut(s)	391, 510, 1094, 1919, 2034
Ecl136II	GAGCTC	3 cut(s)	717, 996, 1109
Eco130I	CCWWGG	3 cut(s)	1548, 1759, 1865
Eco147I	AGGCCT	1 cut(s)	1407
Eco24I	GRGCYC	5 cut(s)	522, 719, 998, 1111, 2029
Eco47I	GGWCC	3 cut(s)	478, 641, 1190
Eco53kI	GAGCTC	3 cut(s)	717, 996, 1109
Eco57I	CTGAAG	2 cut(s)	312, 1216
Eco88I	CYCGRG	1 cut(s)	252
EcoICRI	GAGCTC	3 cut(s)	717, 996, 1109
EcoO109I	RGGNCCY	1 cut(s)	296
EcoRI	GAATTC	2 cut(s)	105, 1120
EcoRII	CCWGG	3 cut(s)	176, 1161, 1908
EcoT14I	CCWWGG	3 cut(s)	1548, 1759, 1865
EcoT22I	ATGCAT	1 cut(s)	1716
EcoT38I	GRGCYC	5 cut(s)	522, 719, 998, 1111, 2029
ErhI	CCWWGG	3 cut(s)	1548, 1759, 1865
Esp3I	CGTCTC	1 cut(s)	1309
FalI	AAGNNNNNCTT	6 cut(s)	370, 402, 1392, 1424, 1608, 1640
FaqI	GGGAC	2 cut(s)	829, 1203
FauI	CCCGC	2 cut(s)	1241, 2016
FauNDI	CATATG	1 cut(s)	1687
FblI	GTMKAC	1 cut(s)	848
FokI	GGATG	9 cut(s)	17, 23, 175, 938, 1028, 1042, 1292, 1551, 1622
FriOI	GRGCYC	5 cut(s)	522, 719, 998, 1111, 2029
FspBI	CTAG	2 cut(s)	98, 390
FspI	TGCGCA	1 cut(s)	1803
GlaI	GCGC	2 cut(s)	201, 1803
GsuI	CTGGAG	1 cut(s)	1299
HaeIII	GGCC	5 cut(s)	297, 315, 1407, 1809, 1908
HapII	CCGG	3 cut(s)	1335, 1459, 1589
HgaI	GACGC	1 cut(s)	191
HhaI	GCGC	2 cut(s)	202, 1804
Hin6I	GCGC	2 cut(s)	200, 1802
HinP1I	GCGC	2 cut(s)	200, 1802
HincII	GTYRAC	2 cut(s)	575, 1050
HindII	GTYRAC	2 cut(s)	575, 1050
HinfI	GANTC	9 cut(s)	52, 242, 658, 694, 708, 772, 971, 1019, 1533
HpaII	CCGG	3 cut(s)	1335, 1459, 1589
HphI	GGTGA	3 cut(s)	635, 851, 1960
Hpy166II	GTNNAC	5 cut(s)	395, 575, 849, 1050, 1622
Hpy188III	TCNNGA	9 cut(s)	712, 893, 1277, 1454, 1559, 1577, 1607, 1746, 2036
Hpy8I	GTNNAC	5 cut(s)	395, 575, 849, 1050, 1622
HpyAV	CCTTC	6 cut(s)	139, 309, 645, 680, 1006, 1610
HpyCH4III	ACNGT	8 cut(s)	335, 347, 512, 754, 922, 1077, 1264, 1417
HpyCH4IV	ACGT	2 cut(s)	757, 1135
HpyF10VI	GCNNNNNNNGC	5 cut(s)	125, 442, 603, 1553, 1711
HpySE526I	ACGT	2 cut(s)	757, 1135
HspAI	GCGC	2 cut(s)	200, 1802
KpnI	GGTACC	1 cut(s)	921
Kzo9I	GATC	4 cut(s)	61, 545, 1348, 1699
LguI	GCTCTTC	3 cut(s)	391, 510, 2034
LmnI	GCTCC	5 cut(s)	226, 731, 1106, 1861, 1939
Lsp1109I	GCAGC	9 cut(s)	79, 335, 377, 548, 615, 949, 1918, 1945, 1967
LweI	GCATC	7 cut(s)	352, 755, 937, 1057, 1270, 1534, 1600
MaeI	CTAG	2 cut(s)	98, 390
MaeII	ACGT	2 cut(s)	757, 1135
MaeIII	GTNAC	3 cut(s)	203, 472, 1444
MalI	GATC	4 cut(s)	63, 547, 1350, 1701
MboI	GATC	4 cut(s)	61, 545, 1348, 1699
MfeI	CAATTG	1 cut(s)	37
MflI	RGATCY	1 cut(s)	545
MhlI	GDGCHC	7 cut(s)	397, 522, 719, 998, 1111, 1792, 2029
MlsI	TGGCCA	1 cut(s)	1908
MluNI	TGGCCA	1 cut(s)	1908
MlyI	GAGTC	2 cut(s)	781, 1028
MmeI	TCCRAC	4 cut(s)	80, 82, 140, 443
Mox20I	TGGCCA	1 cut(s)	1908
Mph1103I	ATGCAT	1 cut(s)	1716
MscI	TGGCCA	1 cut(s)	1908
MseI	TTAA	5 cut(s)	267, 285, 678, 882, 1478
MslI	CAYNNNNRTG	2 cut(s)	798, 945
Msp20I	TGGCCA	1 cut(s)	1908
MspA1I	CMGCKG	4 cut(s)	368, 863, 1442, 1958
MspI	CCGG	3 cut(s)	1335, 1459, 1589
MspR9I	CCNGG	4 cut(s)	178, 1163, 1589, 1910
MunI	CAATTG	1 cut(s)	37
Mva1269I	GAATGC	1 cut(s)	1913
MvaI	CCWGG	3 cut(s)	178, 1163, 1910
MvnI	CGCG	1 cut(s)	202
MwoI	GCNNNNNNNGC	5 cut(s)	125, 442, 603, 1553, 1711
NciI	CCSGG	1 cut(s)	1589
NdeI	CATATG	1 cut(s)	1687
NdeII	GATC	4 cut(s)	61, 545, 1348, 1699
NlaIV	GGNNCC	9 cut(s)	532, 547, 733, 919, 1191, 1224, 1757, 1863, 1941
NmeAIII	GCCGAG	1 cut(s)	291
NmuCI	GTSAC	1 cut(s)	203
NsbI	TGCGCA	1 cut(s)	1803
NsiI	ATGCAT	1 cut(s)	1716
NspI	RCATGY	3 cut(s)	590, 610, 1439
PaeI	GCATGC	1 cut(s)	1439
PagI	TCATGA	1 cut(s)	892
PceI	AGGCCT	1 cut(s)	1407
PciI	ACATGT	1 cut(s)	586
PciSI	GCTCTTC	3 cut(s)	391, 510, 2034
PctI	GAATGC	1 cut(s)	1913
PfeI	GAWTC	7 cut(s)	52, 242, 658, 694, 708, 971, 1533
PflMI	CCANNNNNTGG	1 cut(s)	704
PinAI	ACCGGT	1 cut(s)	1458
PleI	GAGTC	2 cut(s)	780, 1027
PpsI	GAGTC	2 cut(s)	780, 1027
Ppu21I	YACGTR	1 cut(s)	758
PscI	ACATGT	1 cut(s)	586
PsiI	TTATAA	1 cut(s)	1602
Psp124BI	GAGCTC	3 cut(s)	719, 998, 1111
Psp1406I	AACGTT	1 cut(s)	1135
Psp6I	CCWGG	3 cut(s)	176, 1161, 1908
PspGI	CCWGG	3 cut(s)	176, 1161, 1908
PspN4I	GGNNCC	9 cut(s)	532, 547, 733, 919, 1191, 1224, 1757, 1863, 1941
PspPI	GGNCC	6 cut(s)	296, 314, 478, 641, 1190, 1807
PsuI	RGATCY	1 cut(s)	545
PvuII	CAGCTG	2 cut(s)	863, 1958
RsaI	GTAC	9 cut(s)	585, 756, 788, 835, 919, 1208, 1261, 1623, 1885
RsaNI	GTAC	9 cut(s)	584, 755, 787, 834, 918, 1207, 1260, 1622, 1884
RseI	CAYNNNNRTG	2 cut(s)	798, 945
SacI	GAGCTC	3 cut(s)	719, 998, 1111
SapI	GCTCTTC	3 cut(s)	391, 510, 2034
SaqAI	TTAA	5 cut(s)	267, 285, 678, 882, 1478
Sau3AI	GATC	4 cut(s)	61, 545, 1348, 1699
Sau96I	GGNCC	6 cut(s)	296, 314, 478, 641, 1190, 1807
SchI	GAGTC	2 cut(s)	781, 1028
ScrFI	CCNGG	4 cut(s)	178, 1163, 1589, 1910
SduI	GDGCHC	7 cut(s)	397, 522, 719, 998, 1111, 1792, 2029
SfaNI	GCATC	7 cut(s)	352, 755, 937, 1057, 1270, 1534, 1600
SfcI	CTRYAG	5 cut(s)	114, 502, 523, 750, 1769
SinI	GGWCC	3 cut(s)	478, 641, 1190
SmiMI	CAYNNNNRTG	2 cut(s)	798, 945
SmlI	CTYRAG	3 cut(s)	712, 1727, 1979
SmoI	CTYRAG	3 cut(s)	712, 1727, 1979
SphI	GCATGC	1 cut(s)	1439
SseBI	AGGCCT	1 cut(s)	1407
SsiI	CCGC	7 cut(s)	368, 1248, 1440, 1505, 1703, 1835, 2023
SspI	AATATT	2 cut(s)	1144, 1636
SspMI	CTAG	2 cut(s)	98, 390
SstI	GAGCTC	3 cut(s)	719, 998, 1111
StuI	AGGCCT	1 cut(s)	1407
StyD4I	CCNGG	4 cut(s)	176, 1161, 1587, 1908
StyI	CCWWGG	3 cut(s)	1548, 1759, 1865
TaaI	ACNGT	8 cut(s)	335, 347, 512, 754, 922, 1077, 1264, 1417
TaiI	ACGT	2 cut(s)	760, 1138
TaqI	TCGA	1 cut(s)	1792
TatI	WGTACW	4 cut(s)	786, 1206, 1621, 1883
TauI	GCSGC	3 cut(s)	371, 1442, 1838
TfiI	GAWTC	7 cut(s)	52, 242, 658, 694, 708, 971, 1533
Tru1I	TTAA	5 cut(s)	267, 285, 678, 882, 1478
Tru9I	TTAA	5 cut(s)	267, 285, 678, 882, 1478
TscAI	CASTG	3 cut(s)	1131, 1395, 1420
TseFI	GTSAC	1 cut(s)	203
TseI	GCWGC	9 cut(s)	92, 323, 365, 561, 603, 937, 1931, 1955, 1958
Tsp45I	GTSAC	1 cut(s)	203
TspGWI	ACGGA	4 cut(s)	449, 491, 633, 1042
TspRI	CASTG	3 cut(s)	1131, 1395, 1420
Van91I	CCANNNNNTGG	1 cut(s)	704
VneI	GTGCAC	1 cut(s)	393
VpaK11BI	GGWCC	3 cut(s)	478, 641, 1190
XapI	RAATTY	4 cut(s)	105, 1120, 1297, 1511
XceI	RCATGY	3 cut(s)	590, 610, 1439
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1555
XmiI	GTMKAC	1 cut(s)	848
XspI	CTAG	2 cut(s)	98, 390
Zsp2I	ATGCAT	1 cut(s)	1716

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.