FvH4_5g17810

Calmodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016482
Physical Location & Seq
Reverse (-)
10203036 .. 10205687
2652 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g17810.t1

Sequence Viewer

Length: 450 bp
ATGACGGAGCAGCTGACGGAGGAGCAGATCGCCGAGTTCAAGGAAGCCTTTTGCCTCTTCGACAAAGACGGTGATGGTTGCATCACTACCAAGGAATTGGGGACTGTGATGAGATCTCTGGGGCAAAATCCCACTGAAGCTGAATTGCAAGACATGATCAATGAGGTTGATGCCGACCAGAACGGCACAATTGATTTTTCTGAATTCCTGAATTTGATGGCTAGGAAAATGAAGGATACCGATTCTGAGGAGGAACTAAGAGAAGCCTTCAAGGTCTTTGATAAGGATCAGAATGGCTATATTTCTGCTGCAGAGCTTCGACATGTAATGGCAAACCTTGGAGAGAAACTGACTGATGAGGAAGTGGATGAGATGATTCGCGAAGCAGATGAAGATGGTGATGGCCAGGTGAACTATGACGAGTTTGTCAGGATGATGCTTAACAAATGA

Protein Analysis

150

Amino Acids

17.05

Weight (kDa)

4.12

Isoelectric Point (pI)

35.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIF-1 PF21008 7 - 76 5.2e-09 Allograft inflammatory factor 1
EF-hand_7 PF13499 12 - 73 6.2e-17 EF-hand domain pair
EF-hand_1 PF00036 12 - 40 1.1e-08 EF hand domain
EF-hand_6 PF13405 12 - 41 6.3e-09 EF-hand domain
EF-hand_9 PF14658 16 - 75 5.4e-09 EF-hand domain
EF-hand_8 PF13833 25 - 75 2.8e-11 EF-hand domain pair
EF-hand_1 PF00036 48 - 75 4.4e-08 EF hand domain
EF-hand_7 PF13499 83 - 146 1.7e-21 EF-hand domain pair
AIF-1 PF21008 84 - 149 5.2e-07 Allograft inflammatory factor 1
EF-hand_1 PF00036 85 - 112 3.5e-09 EF hand domain
EF-hand_6 PF13405 85 - 114 1.3e-09 EF-hand domain
EF-hand_5 PF13202 86 - 110 4e-06 EF hand
EF-hand_9 PF14658 89 - 147 1e-06 EF-hand domain
EF-hand_8 PF13833 97 - 147 2.2e-15 EF-hand domain pair
EF-hand_1 PF00036 121 - 146 6.9e-09 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016482)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17810
malus_domestica MD06G1234300.v1.1 MD14G1241000.v1.1
prunus_persica Prupe.5G238800_v2.0.a1
pyrus_communis pycom06g20900 pycom14g20250
rosa_chinensis RchiOBHm_Chr7g0177771
rosa_laevigata RLG00000005505
rosa_multiflora Rmu_sc0006214.1_g000014
rosa_roxburghii Rroxscaffold_3G00275570
rosa_rugosa Rorug06G0409400
rosa_samantha Rh7AG009000 Rh7BG009100 Rh7DG009100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 425
AccII CGCG 1 cut(s) 381
AclWI GGATC 1 cut(s) 294
AcoI YGGCCR 1 cut(s) 403
AcsI RAATTY 2 cut(s) 203, 211
AcuI CTGAAG 1 cut(s) 156
AflIII ACRYGT 1 cut(s) 322
AgsI TTSAA 2 cut(s) 40, 271
AjnI CCWGG 1 cut(s) 405
AluBI AGCT 3 cut(s) 13, 140, 316
AluI AGCT 3 cut(s) 13, 140, 316
AlwI GGATC 1 cut(s) 294
AoxI GGCC 1 cut(s) 403
ApeKI GCWGC 2 cut(s) 10, 308
ApoI RAATTY 2 cut(s) 203, 211
AsuHPI GGTGA 3 cut(s) 83, 410, 421
BalI TGGCCA 1 cut(s) 405
BbvI GCAGC 2 cut(s) 22, 295
BccI CCATC 4 cut(s) 68, 211, 389, 395
BceAI ACGGC 1 cut(s) 199
BciT130I CCWGG 1 cut(s) 407
BciVI GTATCC 1 cut(s) 229
BclI TGATCA 1 cut(s) 156
BfaI CTAG 1 cut(s) 222
BfmI CTRYAG 1 cut(s) 309
BfuI GTATCC 1 cut(s) 229
BglII AGATCT 1 cut(s) 113
BisI GCNGC 2 cut(s) 11, 309
BlsI GCNGC 2 cut(s) 12, 310
Bme1390I CCNGG 1 cut(s) 407
BmrFI CCNGG 1 cut(s) 407
BmsI GCATC 3 cut(s) 90, 160, 426
BsaBI GATNNNNATC 1 cut(s) 285
BsaJI CCNNGG 2 cut(s) 90, 337
Bse8I GATNNNNATC 1 cut(s) 285
BseBI CCWGG 1 cut(s) 407
BseDI CCNNGG 2 cut(s) 90, 337
BseGI GGATG 2 cut(s) 373, 438
BseJI GATNNNNATC 1 cut(s) 285
BseMII CTCAG 1 cut(s) 237
BseRI GAGGAG 2 cut(s) 35, 263
BseXI GCAGC 2 cut(s) 22, 295
Bsh1236I CGCG 1 cut(s) 381
BshFI GGCC 1 cut(s) 405
BslFI GGGAC 1 cut(s) 115
BsmFI GGGAC 1 cut(s) 115
BsnI GGCC 1 cut(s) 405
Bsp143I GATC 4 cut(s) 27, 113, 156, 286
Bsp68I TCGCGA 1 cut(s) 381
BspANI GGCC 1 cut(s) 405
BspCNI CTCAG 1 cut(s) 238
BspFNI CGCG 1 cut(s) 381
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 1 cut(s) 294
BssECI CCNNGG 2 cut(s) 90, 337
BssMI GATC 4 cut(s) 27, 113, 156, 286
BssT1I CCWWGG 2 cut(s) 90, 337
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 2 cut(s) 71, 106
Bst6I CTCTTC 1 cut(s) 62
BstDEI CTNAG 2 cut(s) 246, 257
BstF5I GGATG 2 cut(s) 373, 438
BstFNI CGCG 1 cut(s) 381
BstKTI GATC 4 cut(s) 30, 116, 159, 289
BstMBI GATC 4 cut(s) 27, 113, 156, 286
BstNI CCWGG 1 cut(s) 407
BstNSI RCATGY 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 405
BstSFI CTRYAG 1 cut(s) 309
BstUI CGCG 1 cut(s) 381
BstV1I GCAGC 2 cut(s) 22, 295
BstX2I RGATCY 1 cut(s) 113
BstXI CCANNNNNNTGG 1 cut(s) 97
BstYI RGATCY 1 cut(s) 113
BsuI GTATCC 1 cut(s) 229
BsuRI GGCC 1 cut(s) 405
BtsCI GGATG 2 cut(s) 373, 438
BtsIMutI CAGTG 1 cut(s) 132
BtuMI TCGCGA 1 cut(s) 381
CviAII CATG 2 cut(s) 154, 323
CviJI RGCY 8 cut(s) 13, 47, 140, 221, 266, 297, 316, 405
CviKI_1 RGCY 8 cut(s) 13, 47, 140, 221, 266, 297, 316, 405
DdeI CTNAG 2 cut(s) 246, 257
DpnI GATC 4 cut(s) 29, 115, 158, 288
DpnII GATC 4 cut(s) 27, 113, 156, 286
DrdI GACNNNNNNGTC 1 cut(s) 425
DseDI GACNNNNNNGTC 1 cut(s) 425
EaeI YGGCCR 1 cut(s) 403
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco130I CCWWGG 2 cut(s) 90, 337
Eco57I CTGAAG 1 cut(s) 156
EcoRI GAATTC 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 405
EcoT14I CCWWGG 2 cut(s) 90, 337
ErhI CCWWGG 2 cut(s) 90, 337
FaeI CATG 2 cut(s) 157, 326
FaiI YATR 4 cut(s) 155, 300, 324, 417
FalI AAGNNNNNCTT 2 cut(s) 32, 64
FaqI GGGAC 1 cut(s) 115
FatI CATG 2 cut(s) 153, 322
FbaI TGATCA 1 cut(s) 156
Fnu4HI GCNGC 2 cut(s) 11, 309
FokI GGATG 2 cut(s) 380, 445
Fsp4HI GCNGC 2 cut(s) 11, 309
FspBI CTAG 1 cut(s) 222
GluI GCNGC 2 cut(s) 11, 309
HaeIII GGCC 1 cut(s) 405
Hin1II CATG 2 cut(s) 157, 326
HinfI GANTC 2 cut(s) 242, 376
HphI GGTGA 3 cut(s) 83, 410, 421
Hpy166II GTNNAC 1 cut(s) 412
Hpy188I TCNGA 3 cut(s) 202, 247, 291
Hpy188III TCNNGA 3 cut(s) 208, 380, 430
Hpy8I GTNNAC 1 cut(s) 412
HpyAV CCTTC 2 cut(s) 226, 277
HpyCH4III ACNGT 2 cut(s) 71, 106
HpyCH4V TGCA 3 cut(s) 81, 148, 311
HpyF3I CTNAG 2 cut(s) 246, 257
Hsp92II CATG 2 cut(s) 157, 326
Ksp22I TGATCA 1 cut(s) 156
Kzo9I GATC 4 cut(s) 27, 113, 156, 286
LmnI GCTCC 2 cut(s) 7, 22
LpnPI CCDG 6 cut(s) 104, 191, 221, 392, 415, 419
Lsp1109I GCAGC 2 cut(s) 22, 295
LweI GCATC 3 cut(s) 90, 160, 426
MaeI CTAG 1 cut(s) 222
MalI GATC 4 cut(s) 29, 115, 158, 288
MboI GATC 4 cut(s) 27, 113, 156, 286
MboII GAAGA 2 cut(s) 49, 404
MfeI CAATTG 1 cut(s) 189
MflI RGATCY 1 cut(s) 113
MlsI TGGCCA 1 cut(s) 405
MluCI AATT 5 cut(s) 95, 143, 189, 203, 211
MluNI TGGCCA 1 cut(s) 405
MnlI CCTC 6 cut(s) 13, 65, 157, 241, 244, 352
Mox20I TGGCCA 1 cut(s) 405
MscI TGGCCA 1 cut(s) 405
MseI TTAA 1 cut(s) 441
Msp20I TGGCCA 1 cut(s) 405
MspA1I CMGCKG 1 cut(s) 13
MspR9I CCNGG 1 cut(s) 407
MunI CAATTG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 407
MvnI CGCG 1 cut(s) 381
NdeII GATC 4 cut(s) 27, 113, 156, 286
NlaIII CATG 2 cut(s) 157, 326
NmeAIII GCCGAG 1 cut(s) 58
NruI TCGCGA 1 cut(s) 381
NspI RCATGY 1 cut(s) 326
PciI ACATGT 1 cut(s) 322
PfeI GAWTC 2 cut(s) 242, 376
PkrI GCNGC 2 cut(s) 12, 310
PscI ACATGT 1 cut(s) 322
Psp6I CCWGG 1 cut(s) 405
PspGI CCWGG 1 cut(s) 405
PstI CTGCAG 1 cut(s) 313
PsuI RGATCY 1 cut(s) 113
PvuII CAGCTG 1 cut(s) 13
RruI TCGCGA 1 cut(s) 381
SaqAI TTAA 1 cut(s) 441
SatI GCNGC 2 cut(s) 11, 309
Sau3AI GATC 4 cut(s) 27, 113, 156, 286
ScrFI CCNGG 1 cut(s) 407
SetI ASST 7 cut(s) 15, 142, 168, 276, 318, 339, 411
SfaNI GCATC 3 cut(s) 90, 160, 426
SfcI CTRYAG 1 cut(s) 309
Sse9I AATT 5 cut(s) 95, 143, 189, 203, 211
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 1 cut(s) 405
StyI CCWWGG 2 cut(s) 90, 337
TaaI ACNGT 2 cut(s) 71, 106
TaqI TCGA 2 cut(s) 60, 319
TasI AATT 5 cut(s) 95, 143, 189, 203, 211
TfiI GAWTC 2 cut(s) 242, 376
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TscAI CASTG 1 cut(s) 139
TseI GCWGC 2 cut(s) 10, 308
TspDTI ATGAA 2 cut(s) 245, 405
TspGWI ACGGA 2 cut(s) 20, 32
TspRI CASTG 1 cut(s) 139
XapI RAATTY 2 cut(s) 203, 211
XceI RCATGY 1 cut(s) 326
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.