FvH4_5g23970

RINT-1 / TIP-1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008466
Physical Location & Seq
Reverse (-)
15254308 .. 15258330
4023 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23970.t1

Sequence Viewer

Length: 2352 bp
ATGGAACACCCTCCTTCTTCTTCTCCTTGTTCATGTCTGCCCAAACCCAGTGACCTCTCCGACAACCAAGTGAGGTTTCTGAACGAGAAGTTCCAAACCCACCAAGATCTTGTCCACAAAGCTCCTCCTCTTCACACCTCAGTCCGCACCCACTCCTCTCTTTTGGATTCTCATCTTCAACACCTCAAATCCACTCTCTCCCATCTCACTGTTTCATGGATTCGTCGCTCTTTTTCTGCCAAAACCAATCTTCACAATCTCGATATCAGTCTACAGAATCTCAGCCTCGTCACCTCCCAAGGTGGGAGTGGTTGGAAAAAGTTGCAGAAGGTTTTGGGGACTGAACTGCCTCAGCTTGCAAAGGAAGTAAAGGGAATAGAGAACATTCGCAGTTATCTTGAGACTACTCTACAGTTAGAAGCTCTGGTTGGAGATCTAGAAGATGCAGTTTTCTGTTTTGTGAATAGCCATAGTGGAAAAATGTTCTCTGCAATGCTATCAAATTCATCTAACTATGAAACAAAGCAGGAAAAGTTGCTTCAAGCCATAAAAGCTTTGAATGATTTGGAAGTGTTGGTTGGCCTAGTAAAACTCCGACCGCAGTGGCATCATCTTCTGAAATCTGTTGATGCTAGGGTGGACAAAACTTTGGCTATTCTTCGTCCACAAGTCATTGCAGATCACCGAGCACTTCTCGCTTCTGTTGGATGGCCACCAAAACTTTCAGCATTAAAAATAGAAAGTGAGCTCTCAGGCCTTCCGAACCCATTAGTCTTAATGCAAGGAGAAAAAAGGAAGAGTTATTCAGATAGCTTTTTAGCTCTTTGTGCTTTACAGCATATACAAACAAGGAGGGAAAATCGACAACTCAACCTCTCAGGACAAAAGGAGTGCATAATACAGCTTTGGGCCATTGATGAAATGGTGTCTCCTATTGCATCACGCATGGAATACCATTTCTCAAAGTGGGTTGATCAACCTGAGTTCATATTTGAGCTTGCCTACAAAATCACCAGAGATTTTATTGTAGGTGTGGATGATGTCTTGCAGCCTCTAATTGATAGAGCAAGATTGGTAAGCTACAGTGCGAAAGAGGCTTGGGTTTCTTCCATGGTACAATTGCTTTCTGAATTTTTGTCAAAAAGAATATTCTCTTCTCTTGCCAAAAGATACAAGGAGAAACAGATGAAATCTGAAGTTATACTGTCATGGCTTCATCTCATTGACCTTATTGTTTTATTCGATAAACGGATTCAGTCACTTTTAAGTTCTGAAACCAGTCTGTTTTCAACAGATTTAGACAGGGTGGAGAGTCTCTCTGGCAACACTTCAGTTTTGATGATATTTGGTAATAGACCAGATTGGCTCAAGATTTGGGCAAAGATAGAGCTCAAGAATGCTTGCAATAAGCTCAAAACAGATTTGAAAGATGAAAGAGCTTGGACTGTAGATGGCAAAGAGGGAGCTGAGTTGCCCTTTGATACACAACATTTTCTTATTTCTACTAGAGAAGACCATAAAGCCCCGCTAATTGCAGAGTCTGCCCTTAGAATCACATGGGAGATGGTTGAAAGATGCCAGACTATGCCTGTCATTTTACCACGTTTACAGTTCATTAGATTAACTGCTGTCAGATTCCTGTGGTACTTTTTTAAAGTATTGCTTGTACAATGCAAGAGAACTGAAATTACTCCTGACAATCCTGATGATGAGGCCTTAGTTAGAGTATGTGGATCAATTAATGCTGCCAAATACATAGAATTGAGGCTTCGACAGTGGAGTGATGATGTAAACTTTTTGGAGATGAAACTTGCTGAAAATGAACCTAGCATCCGGAGAAAAGATAAGAAGACTGATATTAATTTTTTTGGGGAAGAAATACAAAGCTTGTCTGAGCTCGCCACTAACTGGTTGATGGAGATCATTTCTGTTCTTCTTCATCAGTTTGAAATTCTTTCATGGGAATATGTGCAGAAGATGAAACACTATGATCAACAGCCAGAAGACTTGGCTCCTGTGGAAGCATCAGGAGCTATGGATTTAACTATATCTGTGGATTTTGTTGAACCACTTGATGCTTTGAGATGTCATCTTATTCTTTTGAGAATGACTCTCAATGCCACTGATTTCTTAGATCTGTGGAGAAGTCTAGCTGAGGGGCTTGACCATTTCATATCTCGCAGTAATATCACTTGTGGCATTCATTTTTTTGATAGAGGGATCAATCAGTTTGAAACAGATATGCAAGCTTTGTTCTCAATATTTCAGCCGTTCTGTGTTCGGCCTGAAGCATTTTTCCCTTCTACTATAGAGATTATGAAGGTGTTGAAAATGAAGAAAAGAAAAAGGTGA

Protein Analysis

784

Amino Acids

90.03

Weight (kDa)

6.92

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINT1_TIP1 PF04437 303 - 781 1.6e-29 RINT-1/TIP-1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1908
AccI GTMKAC 1 cut(s) 271
AccIII TCCGGA 1 cut(s) 1833
AciI CCGC 3 cut(s) 145, 599, 1526
AclWI GGATC 2 cut(s) 1741, 2228
AcoI YGGCCR 1 cut(s) 710
AcsI RAATTY 3 cut(s) 502, 1130, 1950
AcuI CTGAAG 3 cut(s) 1215, 1314, 2307
AfaI GTAC 3 cut(s) 1116, 1646, 1668
AfiI CCNNNNNNNGG 2 cut(s) 303, 1908
AjuI GAANNNNNNNTTGG 6 cut(s) 60, 92, 411, 443, 561, 593
AloI GAACNNNNNNTCC 2 cut(s) 1815, 1847
Alw21I GWGCWC 4 cut(s) 691, 750, 1392, 1899
Alw26I GTCTC 3 cut(s) 395, 933, 1319
AlwI GGATC 2 cut(s) 1741, 2228
AlwNI CAGNNNCTG 1 cut(s) 1541
Aor13HI TCCGGA 1 cut(s) 1833
AoxI GGCC 6 cut(s) 580, 710, 754, 909, 1713, 2282
ApeKI GCWGC 2 cut(s) 1048, 1745
ApoI RAATTY 3 cut(s) 502, 1130, 1950
AseI ATTAAT 2 cut(s) 1740, 1860
AspS9I GGNCC 1 cut(s) 909
AsuHPI GGTGA 3 cut(s) 283, 674, 1003
BalI TGGCCA 1 cut(s) 712
BanII GRGCYC 3 cut(s) 750, 1392, 1899
BbsI GAAGAC 3 cut(s) 1518, 1856, 2010
Bbv12I GWGCWC 4 cut(s) 691, 750, 1392, 1899
BbvCI CCTCAGC 2 cut(s) 351, 2154
BbvI GCAGC 2 cut(s) 1060, 1732
BccI CCATC 5 cut(s) 210, 702, 1445, 1558, 1909
BceAI ACGGC 1 cut(s) 2254
BclI TGATCA 2 cut(s) 973, 1990
BcoDI GTCTC 3 cut(s) 395, 933, 1319
BfaI CTAG 6 cut(s) 437, 584, 633, 1506, 1827, 2150
BfmI CTRYAG 5 cut(s) 272, 410, 1081, 1446, 2307
BglII AGATCT 3 cut(s) 106, 433, 2134
BisI GCNGC 2 cut(s) 1049, 1746
BlsI GCNGC 2 cut(s) 1050, 1747
BmgT120I GGNCC 1 cut(s) 909
BmiI GGNNCC 1 cut(s) 2013
BmrI ACTGGG 1 cut(s) 42
BmsI GCATC 8 cut(s) 433, 616, 619, 947, 1565, 1839, 2033, 2065
BmuI ACTGGG 1 cut(s) 42
BpiI GAAGAC 3 cut(s) 1518, 1856, 2010
BplI GAGNNNNNCTC 6 cut(s) 678, 710, 1301, 1333, 2095, 2127
Bpu10I CCTNAGC 2 cut(s) 351, 2154
BpuEI CTTGAG 3 cut(s) 419, 1352, 1376
BsaBI GATNNNNATC 2 cut(s) 171, 1919
BsaJI CCNNGG 2 cut(s) 298, 1110
BsaWI WCCGGW 1 cut(s) 1833
Bsc4I CCNNNNNNNGG 2 cut(s) 303, 1908
Bse1I ACTGG 3 cut(s) 48, 1278, 1913
Bse3DI GCAATG 2 cut(s) 498, 672
Bse8I GATNNNNATC 2 cut(s) 171, 1919
BseAI TCCGGA 1 cut(s) 1833
BseDI CCNNGG 2 cut(s) 298, 1110
BseGI GGATG 3 cut(s) 713, 1042, 1830
BseJI GATNNNNATC 2 cut(s) 171, 1919
BseLI CCNNNNNNNGG 2 cut(s) 303, 1908
BseMI GCAATG 2 cut(s) 498, 672
BseMII CTCAG 9 cut(s) 153, 295, 365, 765, 891, 972, 1458, 1884, 2145
BseNI ACTGG 3 cut(s) 48, 1278, 1913
BseRI GAGGAG 3 cut(s) 114, 117, 145
BseXI GCAGC 2 cut(s) 1060, 1732
BsgI GTGCAG 1 cut(s) 1991
Bsh1285I CGRYCG 1 cut(s) 599
BshFI GGCC 6 cut(s) 582, 712, 756, 911, 1715, 2284
BsiEI CGRYCG 1 cut(s) 599
BsiHKAI GWGCWC 4 cut(s) 691, 750, 1392, 1899
BsiSI CCGG 1 cut(s) 1834
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 2 cut(s) 303, 1908
BsmAI GTCTC 3 cut(s) 395, 933, 1319
BsmFI GGGAC 1 cut(s) 352
BsmI GAATGC 2 cut(s) 1402, 2199
BsnI GGCC 6 cut(s) 582, 712, 756, 911, 1715, 2284
Bsp1286I GDGCHC 4 cut(s) 691, 750, 1392, 1899
Bsp13I TCCGGA 1 cut(s) 1833
Bsp1407I TGTACA 1 cut(s) 1666
Bsp143I GATC 9 cut(s) 106, 433, 679, 973, 1733, 1920, 1990, 2134, 2220
Bsp19I CCATGG 1 cut(s) 1110
BspACI CCGC 3 cut(s) 145, 599, 1526
BspANI GGCC 6 cut(s) 582, 712, 756, 911, 1715, 2284
BspCNI CTCAG 9 cut(s) 152, 294, 364, 764, 890, 973, 1459, 1885, 2146
BspEI TCCGGA 1 cut(s) 1833
BspLI GGNNCC 1 cut(s) 2013
BspPI GGATC 2 cut(s) 1741, 2228
BsrDI GCAATG 2 cut(s) 498, 672
BsrGI TGTACA 1 cut(s) 1666
BsrI ACTGG 3 cut(s) 48, 1278, 1913
BssECI CCNNGG 2 cut(s) 298, 1110
BssMI GATC 9 cut(s) 106, 433, 679, 973, 1733, 1920, 1990, 2134, 2220
BssT1I CCWWGG 2 cut(s) 298, 1110
Bst4CI ACNGT 7 cut(s) 211, 414, 1085, 1206, 1447, 1611, 1776
Bst6I CTCTTC 3 cut(s) 135, 791, 1159
BstAPI GCANNNNNTGC 1 cut(s) 1541
BstAUI TGTACA 1 cut(s) 1666
BstC8I GCNNGC 5 cut(s) 357, 999, 1402, 1899, 2247
BstDSI CCRYGG 1 cut(s) 1110
BstF5I GGATG 3 cut(s) 713, 1042, 1830
BstKTI GATC 9 cut(s) 109, 436, 682, 976, 1736, 1923, 1993, 2137, 2223
BstMAI GTCTC 3 cut(s) 395, 933, 1319
BstMBI GATC 9 cut(s) 106, 433, 679, 973, 1733, 1920, 1990, 2134, 2220
BstMCI CGRYCG 1 cut(s) 599
BstMWI GCNNNNNNNGC 6 cut(s) 551, 695, 827, 1094, 1541, 2030
BstSFI CTRYAG 5 cut(s) 272, 410, 1081, 1446, 2307
BstV1I GCAGC 2 cut(s) 1060, 1732
BstV2I GAAGAC 3 cut(s) 1518, 1856, 2010
BstX2I RGATCY 3 cut(s) 106, 433, 2134
BstYI RGATCY 3 cut(s) 106, 433, 2134
BsuRI GGCC 6 cut(s) 582, 712, 756, 911, 1715, 2284
BtgI CCRYGG 1 cut(s) 1110
BtsCI GGATG 3 cut(s) 713, 1042, 1830
BtsI GCAGTG 1 cut(s) 608
BtsIMutI CAGTG 6 cut(s) 55, 207, 608, 1090, 1781, 2121
Cac8I GCNNGC 5 cut(s) 357, 999, 1402, 1899, 2247
CaiI CAGNNNCTG 1 cut(s) 1541
Cfr13I GGNCC 1 cut(s) 909
Csp6I GTAC 3 cut(s) 1115, 1645, 1667
CviAII CATG 7 cut(s) 33, 216, 946, 1111, 1209, 1557, 1959
CviQI GTAC 3 cut(s) 1115, 1645, 1667
DpnI GATC 9 cut(s) 108, 435, 681, 975, 1735, 1922, 1992, 2136, 2222
DpnII GATC 9 cut(s) 106, 433, 679, 973, 1733, 1920, 1990, 2134, 2220
DraI TTTAAA 1 cut(s) 1654
EaeI YGGCCR 1 cut(s) 710
Eam1104I CTCTTC 3 cut(s) 135, 791, 1159
EarI CTCTTC 3 cut(s) 135, 791, 1159
Ecl136II GAGCTC 3 cut(s) 748, 1390, 1897
Eco130I CCWWGG 2 cut(s) 298, 1110
Eco147I AGGCCT 2 cut(s) 756, 1715
Eco24I GRGCYC 3 cut(s) 750, 1392, 1899
Eco32I GATATC 1 cut(s) 265
Eco53kI GAGCTC 3 cut(s) 748, 1390, 1897
Eco57I CTGAAG 3 cut(s) 1215, 1314, 2307
EcoICRI GAGCTC 3 cut(s) 748, 1390, 1897
EcoRV GATATC 1 cut(s) 265
EcoT14I CCWWGG 2 cut(s) 298, 1110
EcoT38I GRGCYC 3 cut(s) 750, 1392, 1899
ErhI CCWWGG 2 cut(s) 298, 1110
FaeI CATG 7 cut(s) 36, 219, 949, 1114, 1212, 1560, 1962
FalI AAGNNNNNCTT 2 cut(s) 1647, 1679
FaqI GGGAC 1 cut(s) 352
FatI CATG 7 cut(s) 32, 215, 945, 1110, 1208, 1556, 1958
FauI CCCGC 1 cut(s) 1533
FbaI TGATCA 2 cut(s) 973, 1990
FblI GTMKAC 1 cut(s) 271
Fnu4HI GCNGC 2 cut(s) 1049, 1746
FokI GGATG 3 cut(s) 720, 1049, 1817
FriOI GRGCYC 3 cut(s) 750, 1392, 1899
Fsp4HI GCNGC 2 cut(s) 1049, 1746
FspBI CTAG 6 cut(s) 437, 584, 633, 1506, 1827, 2150
GluI GCNGC 2 cut(s) 1049, 1746
HaeIII GGCC 6 cut(s) 582, 712, 756, 911, 1715, 2284
HapII CCGG 1 cut(s) 1834
Hin1II CATG 7 cut(s) 36, 219, 949, 1114, 1212, 1560, 1962
HindIII AAGCTT 3 cut(s) 552, 1885, 2247
HinfI GANTC 9 cut(s) 167, 220, 277, 1252, 1312, 1538, 1551, 1635, 2110
HpaII CCGG 1 cut(s) 1834
HphI GGTGA 3 cut(s) 283, 674, 1003
Hpy166II GTNNAC 6 cut(s) 115, 272, 640, 665, 1607, 1792
Hpy8I GTNNAC 6 cut(s) 115, 272, 640, 665, 1607, 1792
Hpy99I CGWCG 1 cut(s) 228
HpyAV CCTTC 5 cut(s) 24, 322, 767, 2310, 2314
HpyCH4III ACNGT 7 cut(s) 211, 414, 1085, 1206, 1447, 1611, 1776
HpyCH4IV ACGT 1 cut(s) 1603
HpyF10VI GCNNNNNNNGC 6 cut(s) 551, 695, 827, 1094, 1541, 2030
HpySE526I ACGT 1 cut(s) 1603
Hsp92II CATG 7 cut(s) 36, 219, 949, 1114, 1212, 1560, 1962
Kpn2I TCCGGA 1 cut(s) 1833
Ksp22I TGATCA 2 cut(s) 973, 1990
Kzo9I GATC 9 cut(s) 106, 433, 679, 973, 1733, 1920, 1990, 2134, 2220
LmnI GCTCC 4 cut(s) 127, 1463, 2017, 2030
Lsp1109I GCAGC 2 cut(s) 1060, 1732
LweI GCATC 8 cut(s) 433, 616, 619, 947, 1565, 1839, 2033, 2065
MaeI CTAG 6 cut(s) 437, 584, 633, 1506, 1827, 2150
MaeII ACGT 1 cut(s) 1603
MaeIII GTNAC 3 cut(s) 50, 289, 1257
MalI GATC 9 cut(s) 108, 435, 681, 975, 1735, 1922, 1992, 2136, 2222
MboI GATC 9 cut(s) 106, 433, 679, 973, 1733, 1920, 1990, 2134, 2220
MfeI CAATTG 1 cut(s) 1118
MflI RGATCY 3 cut(s) 106, 433, 2134
MhlI GDGCHC 4 cut(s) 691, 750, 1392, 1899
MlsI TGGCCA 1 cut(s) 712
MluNI TGGCCA 1 cut(s) 712
MlyI GAGTC 3 cut(s) 1321, 1547, 2104
MmeI TCCRAC 5 cut(s) 84, 293, 409, 619, 685
Mox20I TGGCCA 1 cut(s) 712
MroI TCCGGA 1 cut(s) 1833
MscI TGGCCA 1 cut(s) 712
MseI TTAA 8 cut(s) 731, 776, 1265, 1622, 1653, 1740, 1860, 2042
Msp20I TGGCCA 1 cut(s) 712
MspI CCGG 1 cut(s) 1834
MunI CAATTG 1 cut(s) 1118
Mva1269I GAATGC 2 cut(s) 1402, 2199
MwoI GCNNNNNNNGC 6 cut(s) 551, 695, 827, 1094, 1541, 2030
NcoI CCATGG 1 cut(s) 1110
NdeII GATC 9 cut(s) 106, 433, 679, 973, 1733, 1920, 1990, 2134, 2220
NlaIII CATG 7 cut(s) 36, 219, 949, 1114, 1212, 1560, 1962
NlaIV GGNNCC 1 cut(s) 2013
NmuCI GTSAC 3 cut(s) 50, 289, 1257
PceI AGGCCT 2 cut(s) 756, 1715
PctI GAATGC 2 cut(s) 1402, 2199
PfeI GAWTC 6 cut(s) 167, 220, 277, 1252, 1551, 1635
PflMI CCANNNNNTGG 1 cut(s) 1908
PkrI GCNGC 2 cut(s) 1050, 1747
PleI GAGTC 3 cut(s) 1320, 1546, 2104
PpsI GAGTC 3 cut(s) 1320, 1546, 2104
PshBI ATTAAT 2 cut(s) 1740, 1860
Psp124BI GAGCTC 3 cut(s) 750, 1392, 1899
PspN4I GGNNCC 1 cut(s) 2013
PspPI GGNCC 1 cut(s) 909
PsrI GAACNNNNNNTAC 2 cut(s) 1672, 1704
PstNI CAGNNNCTG 1 cut(s) 1541
PsuI RGATCY 3 cut(s) 106, 433, 2134
RsaI GTAC 3 cut(s) 1116, 1646, 1668
RsaNI GTAC 3 cut(s) 1115, 1645, 1667
SacI GAGCTC 3 cut(s) 750, 1392, 1899
SaqAI TTAA 8 cut(s) 731, 776, 1265, 1622, 1653, 1740, 1860, 2042
SatI GCNGC 2 cut(s) 1049, 1746
Sau3AI GATC 9 cut(s) 106, 433, 679, 973, 1733, 1920, 1990, 2134, 2220
Sau96I GGNCC 1 cut(s) 909
SchI GAGTC 3 cut(s) 1321, 1547, 2104
SduI GDGCHC 4 cut(s) 691, 750, 1392, 1899
SfaNI GCATC 8 cut(s) 433, 616, 619, 947, 1565, 1839, 2033, 2065
SfcI CTRYAG 5 cut(s) 272, 410, 1081, 1446, 2307
SmlI CTYRAG 3 cut(s) 398, 1367, 1391
SmoI CTYRAG 3 cut(s) 398, 1367, 1391
SseBI AGGCCT 2 cut(s) 756, 1715
SsiI CCGC 3 cut(s) 145, 599, 1526
SspI AATATT 2 cut(s) 1149, 2262
SspMI CTAG 6 cut(s) 437, 584, 633, 1506, 1827, 2150
SstI GAGCTC 3 cut(s) 750, 1392, 1899
StuI AGGCCT 2 cut(s) 756, 1715
StyI CCWWGG 2 cut(s) 298, 1110
TaaI ACNGT 7 cut(s) 211, 414, 1085, 1206, 1447, 1611, 1776
TaiI ACGT 1 cut(s) 1606
TaqI TCGA 4 cut(s) 261, 862, 1242, 1771
TatI WGTACW 1 cut(s) 1666
TfiI GAWTC 6 cut(s) 167, 220, 277, 1252, 1551, 1635
Tru1I TTAA 8 cut(s) 731, 776, 1265, 1622, 1653, 1740, 1860, 2042
Tru9I TTAA 8 cut(s) 731, 776, 1265, 1622, 1653, 1740, 1860, 2042
TscAI CASTG 6 cut(s) 55, 214, 608, 1090, 1781, 2128
TseFI GTSAC 3 cut(s) 50, 289, 1257
TseI GCWGC 2 cut(s) 1048, 1745
Tsp45I GTSAC 3 cut(s) 50, 289, 1257
TspGWI ACGGA 1 cut(s) 1264
TspRI CASTG 6 cut(s) 55, 214, 608, 1090, 1781, 2128
Van91I CCANNNNNTGG 1 cut(s) 1908
VspI ATTAAT 2 cut(s) 1740, 1860
XapI RAATTY 3 cut(s) 502, 1130, 1950
XbaI TCTAGA 1 cut(s) 436
XcmI CCANNNNNNNNNTGG 2 cut(s) 305, 919
XmiI GTMKAC 1 cut(s) 271
XspI CTAG 6 cut(s) 437, 584, 633, 1506, 1827, 2150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.