FvH4_6g05850

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015422
Physical Location & Seq
Reverse (-)
3321348 .. 3327080
5733 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g05850.t1

Sequence Viewer

Length: 4674 bp
ATGTACTTGAAATGTGGGCAGTTGTGGGATGCGGTTAAGGTGTTCGATGAAATGCCTGTGAGAGATACGGTTTCGTGGAACACTGTGATATCGGGGTTTTTGAGGAATGGGGAGCCTGAGACTGGTTTTGGGTACTTCAAGAGGATGTTGGAGTCAGATTATTGTCGATTTGATAAAGCTACTTTGACTTGCCTTATAGCGGGTTTTGATGGGGTGGAGGGTTGTTACTTGAACAAGATGATGCATGGTTTGGCGGTTTTGAATGGATTTGAGCGGGAAACTGCAGTGGGGAATGCTTTGATAACATCTTACTGTAAATGTGGGTGCTTTGGCTCAGCAAGGCGGGTTTTTGATGAGATGTTTGAGAGGAATGTAATTACGTGGACTGCAATGATTTCGGGCCTTGCACATTATGGTTTCTATGCTGAGAGCTTGAAGTTGTTTGCGGATATGCTTGGTGGTGTGGTGGAACCGAATGCTATGACGTTTTTGGGCTCACTTATGGCATGTTCTGGATTGCAGGCAATAAGGGAGGGGCGTCAGATCCATGGCCTTGTATGGAAATTGGGAGTTCAATCGGACCTGTGTGTGGAGAGTTCATTGATGGACATGTATTCAAAATGTGGTAGTGTGGAAGATGCATGGAGAATCTTTGAGTCCACTAAAGAACTTGATGAGGTTTCCATGACTGTTATCCTTGTGGGATTTGCACAGAATGGGTTTGAGACAGAAGCTATCCAGATTTTTGTGAAAATGATGAGGGCAGGGATTGAAATTGACCCAAACATGGTTTCAGCAGTTCTTGGAGTATTTGGTGTTGATACTTCTTTAGGTCTGGGAAAGCAACTACACTCTTTGATAGTCAAGAAGAATTTTGGTTATAATTCTTTTGTCACCAATGGGCTAATAAATATGTACTCCAAGTGTGGAGAGCTGGAGGATTCAGAAAAAGTGTTCACTCAAATGCCTCAAAGGAATTCAATTTCATGGAATTCAATGATTGCGGCGTTTGCCCGTCATGGAGATGGCTCCAAAGCATTACAACTGTATGAAAATATGCAAATGGAAGGCGTACAACTGACAGATGTTACATTTCTGTCTCTACTTCATGCTTGTAGCCATGCTGGCTTGATTGAAAGGGGCATGGAATTTCTGAACTTTATGAAAGAAGAACATGGGATGAGTCCAAGGCCAGAACACTATGCTTGTGTTGTTGACATGTTAGGTCGGGCAGGACTTTTAACTGAGGCTAAATCTTTTATTGAAGGACTACCTGAAAATCCTGGGATACTTGTTTGGCAAGCATTACTCGGTGCTTGTAGCATTCATAAAGAATCTGATATTGGGAAATATGCTGCAGATCAGTTGTTTTTGACAGCACCGCAGTGTCCCGCACCATATGTTTTGCTTGCCAATATATATTCTTTTGAAGGAAGGTGGAAAGAGAGAGCAAAGACTATTAAGGGGATGAAGAAGATGGGAGTGGCAAAAGAAGCGGGTATTAGTTGGATTGAAATCGAAAAGAAAGTTCAGAGCTTTGTTGTTGGTGATAAAATGCATCCACAAGCGGAGATCATTTATAGGCTCTTGGCAGATTTGCTTAGACTTATGAAGGATGAAGGATACGTACCGGATGAGAGGGAGGAGCTAACAAGTGACTCCAAAGATCATCTTCAAACTTGGAATGAAGCCAATCCCTTGTGCAACTGGACTGGAGTTACATGCCACCAACGTCTCCAAAACCGGGTTATAGGTCTTGAGCTCATAGATATGGGGTTACAAGGAAAAATATCACCATTCATATCCAATCTCTCTCTACTTGCCAACTTGTCTTTACAGATCAATGGGTTCCATGGACAAATTCCATCTTCCTTTGGAAAACTTACAGAGTTAGCGTTTCTGAATGCTAGCTCAAACAAGCTTGAAGGTAACATTCCAGGTTCATTACATGGTTGCCGAAGCTTGAAAGTCGTAGACTTGACTTACAACAATCTATCTGGTGTCATTCCTGAAGAACTCGGCTGGATGAAGAGTCTGGCATATTTAGCTCTCTCCGAAAACAGTCTCAGGGGAGTGCTTCCAGCCTCTCTTTCAAACTTGACGGAATTGACACAACTTGAACTGGCTGTCAACTATTTTACTGGAAAGATCCCACCAGAGCTCGGAGCATTAAGAAAGCTAGAGATTTTGTATCTCCATATGAACTATCTCGAAGGCTCGATACCGGCAGCAATTGCTAACTGCACTGCTTTACGCGAAATTTCGCTGATTGAGAACTTGTTAAGTGGAGAAATCCCTTCAGCTTTGTTTGCTAAACTCAAGAATATGCAGAAAATGTACATGTCAAGAAACAAGCTTTCTGGGAAAATTCCAGGGACCCTCTCCAATCTTTCACAGCTGATACTGTTTGATGTTAGTTTGAATAATTTAGAGGGGGAAGTTCCTGGAGGATTGGGCATGCTAAAGAACCTAGAGAATTTCTACTTACATTCCAACAACTTGTGTGGCGGCTCTGGTAATTCTTCTCTCAGTTTCTTAACAGCTTTAACTAACTGTTCGTATCTGCAAAAACTACACTTGGGTTCGTGTTTGTTCACTGGAAATCTACCATCTTCTATAGGAAACCTTTCAAAAGACCTGTATTACTTGAATCTTTTTGATAACCACATTACAGGAAGTATGCCAGAAAGTATCGGAAACTTAAGCGGCCTAGTAACATTGTACTTGTCATACAACCGTTTGGTAGGAAGCATACCATCTTCCCTTGGCAAGCTTTTGAATTTGCAGAGGTTATACTTGGGGAACAACAGAATTATTGGGCCCATGCCAGATGAGTTAGGGATGATGTCTAGCCTTGGTGTACTGGATTTTGGCAACAATTCGATTGGTGGGACGATTCCTCCTTCACTTGGTAATCTCTCACAGCTGAGATATCTTTACCTGTCCAGAAATTTCCTATCGGGAATGTTTCCAATTGAGCTTACTCAATGCTCTCTTATGATGCTGCTTGATCTATCTTTCAACAAATTACAAGGCTCTGTTCCTGTAGAGATTGGCGGCTTTTCAAACCTAGCTCTCTCTCTTAACCTTTCGAACAATAAGTTCGAAGGACAGTTGCCTGCAAGCATTGGAAAGCTGGTATCTGTACAGGCAATTGACTTGTCTGAGAACAAGTTTTCTGGTTTGATACCTACCTTGATTGGAAGCTGCATTTCTTTGGCCTATCTAAACATGTCCAACAATATGTTTGAGGGGACTATTCCAGATTCGTTGAGTTTGATCACTCATCTTGAAGTTTTGGACTTGGCTCGTAATGAATTAAATGGCACCCTTCCGAAATGGATTGCTGAAAACCAGATGATCAGGAATCTTAATTTATCTTACAATAGGCTATCAGGAGAAGTTCCAGATACTGGAAGGAGAAATATTTTTAATAGAAGCTCATTGTTGGGGAATGTCGGGTTGTGTGGTGGTTCTGCACTACTTGGTCTTCCGCCATGTGAAGTTCAAAAGAGAAAACATAAGATAAGAAAATCGATAATTTACTCTTTGGTTGCTGCAGTTGCGTTTGTCTGTGTGCTGGTGGCTGTTTTCGTTTATTGTTTCTTCTTCTCCTTCAGAAAGCGGGATTCAAAAGCAGAAGACAGAATGGTAACAGTAATGGGATCACCAAGCCATCATGGAAGGCGCACTTTCACCCAAAGGGAACTTGAACTTGCAACAGGTGAGTTCAGTGAAGCTCATCTGTTGGGTAGAGGAACCTTCGGATCAGTCTATAAGGCAATTGTTGATGATGGTGAAACTATTATGGCAGTGAAGGTTCTGCATGGTGACGGTATTCAGAGTTACAAAAGCTTTAAGAGGGAATGTCAAATCATGTCCGAAATTAAGCACCGGAATCTTGTTAGAATGTTAGGATATACCTGGAGTACACAGTTCAAGGCTCTAATTCTTGAATACATAGGAAATGGTAACTTGGCAGAGCATCTTTATCCTGGCGGACTAGAGGAAGGAACATGTGAACTATCGTTGAGGGAAAGAATATCAATAGCCATAGATGTTGCCAATGGCTTGGAGTATCTTCAAGAAGGTTGTCGAGTCCAAATTCTACACTGTGACTTAAAACCAGAGAATGTGCTTATTGACAATGATATGGTAGCTCATGTAGCAGATTTTGGGATTGGAAAGCTCATATCAGCTGATAAACCCAAGGAACTACATGTTAGCACAACGCATTTTCTACGAGGATCAATTGGGTACATTCCCCCAGAATATGCGCAAGGGATTGAGGTTTCAGCTAAAGGAGATGTCTATAGCTTTGGTGTGATGGTGCTCGAGTTGATAACAAGAAAAAGACCGACAAGCAATATGTTTCGAGATGAGGTTGATCTAAGGAAGTGGGTGCATTCTTCGTACCCAGACCATGTTTTGGATGTTGTTGACAGCACGCTAAAGGAGATAAAAAGCACAGATGGTGCTTTGGATGAGCTTGAGCGATGCTGCATTCAGATGCTTGAGGTAGGGTTGAGGTGCACAGAAGATACTCCACATAAAAGACCCTCCATGTCTTTTGTTGTGCAGAAGTTGACACAGTGCCTAGAAAGCTGGAGAGACCTGGAGACCTGGAGAGATGGAGAGATGGTGAAACTTCTGCATCCACAAATGTTGGAATAG

Protein Analysis

1558

Amino Acids

172.51

Weight (kDa)

5.92

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 21 - 69 5.5e-06 PPR repeat family
PPR PF01535 24 - 52 9.3e-06 PPR repeat
PPR_2 PF13041 326 - 373 1e-10 PPR repeat family
PPR PF01535 328 - 358 5.7e-07 PPR repeat
E_motif PF20431 443 - 505 1.8e-12 E motif
LRRNT_2 PF08263 549 - 575 8.1e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 648 - 758 1.1e-07 Leucine-rich repeat region
LRR_8 PF13855 653 - 711 1.3e-07 Leucine rich repeat
LRR_14 PF23598 843 - 983 3.9e-14 Leucine-rich repeat region
LRR_8 PF13855 880 - 938 1.6e-07 Leucine rich repeat
LRR_8 PF13855 927 - 984 6.9e-07 Leucine rich repeat
Pkinase PF00069 1245 - 1527 1.7e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1248 - 1529 3e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015422)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05340
fragaria_vesca FvH4_6g05850
malus_domestica MD12G1219000.v1.1
prunus_persica Prupe.6G325500_v2.0.a1
pyrus_communis pycom12g20470
rosa_chinensis RchiOBHm_Chr3g0454801
rosa_laevigata RLG00000025406
rosa_multiflora Rmu_sc0001792.1_g000013 Rmu_sc0013126.1_g000006
rosa_roxburghii Rroxscaffold_6G00424720
rosa_rugosa Rorug03G0002600
rosa_samantha Rh3AG062600 Rh3BG064500 Rh3CG063400 Rh3DG064300
rosa_wichuraiana Rw3G004880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 882
Acc16I TGCGCA 1 cut(s) 4278
AccB1I GGYRCC 1 cut(s) 3326
AccB7I CCANNNNNTGG 2 cut(s) 3413, 4429
AccBSI CCGCTC 1 cut(s) 274
AccI GTMKAC 1 cut(s) 1974
AccII CGCG 1 cut(s) 2256
AclWI GGATC 5 cut(s) 538, 2143, 3673, 3775, 4255
AcuI CTGAAG 3 cut(s) 2031, 2283, 3601
AcyI GRCGYC 1 cut(s) 538
AfiI CCNNNNNNNGG 9 cut(s) 122, 199, 589, 787, 1744, 2162, 2909, 3413, 4429
AflII CTTAAG 1 cut(s) 2701
AflIII ACRYGT 6 cut(s) 609, 1218, 2340, 3231, 4016, 4219
AjnI CCWGG 8 cut(s) 1282, 1936, 2371, 2443, 3923, 3994, 4614, 4622
AjuI GAANNNNNNNTTGG 4 cut(s) 775, 807, 1326, 1358
AleI CACNNNNGTG 1 cut(s) 1384
Alw21I GWGCWC 4 cut(s) 1764, 2163, 4335, 4535
Alw26I GTCTC 7 cut(s) 113, 719, 1104, 1739, 2069, 4605, 4613
Alw44I GTGCAC 1 cut(s) 4531
AlwI GGATC 5 cut(s) 538, 2143, 3673, 3775, 4255
AlwNI CAGNNNCTG 1 cut(s) 3413
Ama87I CYCGRG 1 cut(s) 4334
AoxI GGCC 6 cut(s) 400, 550, 1190, 2707, 2819, 3219
ApaI GGGCCC 1 cut(s) 2823
ApaLI GTGCAC 1 cut(s) 4531
ApeKI GCWGC 6 cut(s) 1355, 2228, 3004, 3207, 3557, 4500
Asp700I GAANNNNTTC 1 cut(s) 2626
AspLEI GCGC 2 cut(s) 3690, 4279
AspS9I GGNCC 5 cut(s) 400, 580, 2376, 2819, 2820
AsuC2I CCSGG 1 cut(s) 1745
AsuHPI GGTGA 9 cut(s) 886, 1559, 1785, 3660, 3688, 3737, 3809, 3842, 4654
AsuII TTCGAA 2 cut(s) 3092, 3105
AsuNHI GCTAGC 1 cut(s) 1907
AvaI CYCGRG 1 cut(s) 4334
AvaII GGWCC 2 cut(s) 580, 2376
BaeGI GKGCMC 2 cut(s) 2823, 4535
BanI GGYRCC 1 cut(s) 3326
BanII GRGCYC 4 cut(s) 497, 1764, 2163, 2823
BarI GAAGNNNNNNTAC 4 cut(s) 1611, 1643, 2205, 2237
BbsI GAAGAC 2 cut(s) 3482, 3648
Bbv12I GWGCWC 4 cut(s) 1764, 2163, 4335, 4535
BbvI GCAGC 6 cut(s) 1342, 2240, 2991, 3194, 3544, 4487
BcgI CGANNNNNNTGC 2 cut(s) 378, 412
BciT130I CCWGG 8 cut(s) 1284, 1938, 2373, 2445, 3925, 3996, 4616, 4624
BciVI GTATCC 2 cut(s) 1281, 1616
BclI TGATCA 2 cut(s) 3279, 3360
BcnI CCSGG 1 cut(s) 1745
BcoDI GTCTC 7 cut(s) 113, 719, 1104, 1739, 2069, 4605, 4613
BfaI CTAG 8 cut(s) 1908, 2180, 2471, 2711, 2850, 3071, 4004, 4598
BfmI CTRYAG 6 cut(s) 282, 1356, 2616, 3045, 3558, 4312
BfrI CTTAAG 1 cut(s) 2701
BfuI GTATCC 2 cut(s) 1281, 1616
BglI GCCNNNNNGGC 1 cut(s) 1125
BlpI GCTNAGC 1 cut(s) 334
Bme1390I CCNGG 9 cut(s) 1284, 1745, 1938, 2373, 2445, 3925, 3996, 4616, 4624
Bme18I GGWCC 2 cut(s) 580, 2376
BmeT110I CYCGRG 1 cut(s) 4334
BmgT120I GGNCC 5 cut(s) 400, 580, 2376, 2819, 2820
BmiI GGNNCC 9 cut(s) 114, 471, 1030, 1850, 2377, 2378, 2821, 3328, 3760
BmrFI CCNGG 9 cut(s) 1284, 1745, 1938, 2373, 2445, 3925, 3996, 4616, 4624
BmsI GCATC 9 cut(s) 19, 231, 628, 1567, 2991, 3994, 4487, 4500, 4663
BmtI GCTAGC 1 cut(s) 1911
BpiI GAAGAC 2 cut(s) 3482, 3648
BpmI CTGGAG 7 cut(s) 956, 1734, 2466, 3946, 4627, 4637, 4645
Bpu1102I GCTNAGC 1 cut(s) 334
Bpu14I TTCGAA 2 cut(s) 3092, 3105
BpuEI CTTGAG 4 cut(s) 1778, 2303, 4511, 4535
BpuMI CCSGG 1 cut(s) 1745
Bsa29I ATCGAT 1 cut(s) 3536
BsaAI YACGTR 2 cut(s) 381, 1627
BsaBI GATNNNNATC 1 cut(s) 1514
BsaHI GRCGYC 1 cut(s) 538
BsaI GGTCTC 2 cut(s) 4605, 4613
BsaJI CCNNGG 8 cut(s) 547, 1187, 1283, 1852, 2372, 2764, 2854, 4209
BsaWI WCCGGW 2 cut(s) 1630, 3894
BsaXI ACNNNNNCTCC 4 cut(s) 2884, 2914, 4626, 4656
Bsc4I CCNNNNNNNGG 9 cut(s) 122, 199, 589, 787, 1744, 2162, 2909, 3413, 4429
Bse118I RCCGGY 1 cut(s) 2224
Bse1I ACTGG 8 cut(s) 127, 1712, 1717, 2127, 2146, 2602, 2868, 3418
Bse3DI GCAATG 1 cut(s) 396
Bse8I GATNNNNATC 1 cut(s) 1514
BseBI CCWGG 8 cut(s) 1284, 1938, 2373, 2445, 3925, 3996, 4616, 4624
BseCI ATCGAT 1 cut(s) 3536
BseDI CCNNGG 8 cut(s) 547, 1187, 1283, 1852, 2372, 2764, 2854, 4209
BseJI GATNNNNATC 1 cut(s) 1514
BseLI CCNNNNNNNGG 9 cut(s) 122, 199, 589, 787, 1744, 2162, 2909, 3413, 4429
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 8 cut(s) 108, 348, 417, 1236, 2080, 2542, 2918, 3156
BseNI ACTGG 8 cut(s) 127, 1712, 1717, 2127, 2146, 2602, 2868, 3418
BseRI GAGGAG 1 cut(s) 1658
BseSI GKGCMC 2 cut(s) 2823, 4535
BseXI GCAGC 6 cut(s) 1342, 2240, 2991, 3194, 3544, 4487
BsgI GTGCAG 3 cut(s) 2227, 3462, 4598
Bsh1236I CGCG 1 cut(s) 2256
BshFI GGCC 6 cut(s) 402, 552, 1192, 2709, 2821, 3221
BshNI GGYRCC 1 cut(s) 3326
BshVI ATCGAT 1 cut(s) 3536
BsiHKAI GWGCWC 4 cut(s) 1764, 2163, 4335, 4535
BsiHKCI CYCGRG 1 cut(s) 4334
BsiSI CCGG 4 cut(s) 1631, 1744, 2225, 3895
BslFI GGGAC 4 cut(s) 1374, 2389, 2905, 3268
BslI CCNNNNNNNGG 9 cut(s) 122, 199, 589, 787, 1744, 2162, 2909, 3413, 4429
BsmAI GTCTC 7 cut(s) 113, 719, 1104, 1739, 2069, 4605, 4613
BsmBI CGTCTC 1 cut(s) 1739
BsmFI GGGAC 4 cut(s) 1374, 2389, 2905, 3268
BsmI GAATGC 6 cut(s) 298, 481, 1323, 1909, 4405, 4503
BsnI GGCC 6 cut(s) 402, 552, 1192, 2709, 2821, 3221
Bso31I GGTCTC 2 cut(s) 4605, 4613
BsoBI CYCGRG 1 cut(s) 4334
Bsp119I TTCGAA 2 cut(s) 3092, 3105
Bsp120I GGGCCC 1 cut(s) 2819
Bsp1286I GDGCHC 6 cut(s) 497, 1764, 2163, 2823, 4335, 4535
Bsp1407I TGTACA 2 cut(s) 2337, 3145
Bsp1720I GCTNAGC 1 cut(s) 334
Bsp19I CCATGG 2 cut(s) 547, 1852
BspANI GGCC 6 cut(s) 402, 552, 1192, 2709, 2821, 3221
BspCNI CTCAG 8 cut(s) 109, 347, 418, 1237, 2079, 2541, 2919, 3157
BspDI ATCGAT 1 cut(s) 3536
BspFNI CGCG 1 cut(s) 2256
BspLI GGNNCC 9 cut(s) 114, 471, 1030, 1850, 2377, 2378, 2821, 3328, 3760
BspMAI CTGCAG 3 cut(s) 286, 1360, 3562
BspOI GCTAGC 1 cut(s) 1911
BspPI GGATC 5 cut(s) 538, 2143, 3673, 3775, 4255
BspT104I TTCGAA 2 cut(s) 3092, 3105
BspT107I GGYRCC 1 cut(s) 3326
BspTI CTTAAG 1 cut(s) 2701
BspTNI GGTCTC 2 cut(s) 4605, 4613
BsrBI CCGCTC 1 cut(s) 274
BsrDI GCAATG 1 cut(s) 396
BsrFI RCCGGY 1 cut(s) 2224
BsrGI TGTACA 2 cut(s) 2337, 3145
BsrI ACTGG 8 cut(s) 127, 1712, 1717, 2127, 2146, 2602, 2868, 3418
BssAI RCCGGY 1 cut(s) 2224
BssECI CCNNGG 8 cut(s) 547, 1187, 1283, 1852, 2372, 2764, 2854, 4209
BssNI GRCGYC 1 cut(s) 538
BssT1I CCWWGG 6 cut(s) 547, 1187, 1852, 2764, 2854, 4209
Bst2UI CCWGG 8 cut(s) 1284, 1938, 2373, 2445, 3925, 3996, 4616, 4624
Bst6I CTCTTC 1 cut(s) 2024
BstACI GRCGYC 1 cut(s) 538
BstAFI CTTAAG 1 cut(s) 2701
BstAPI GCANNNNNTGC 1 cut(s) 2234
BstAUI TGTACA 2 cut(s) 2337, 3145
BstBAI YACGTR 2 cut(s) 381, 1627
BstBI TTCGAA 2 cut(s) 3092, 3105
BstDSI CCRYGG 2 cut(s) 547, 1852
BstFNI CGCG 1 cut(s) 2256
BstHHI GCGC 2 cut(s) 3690, 4279
BstMAI GTCTC 7 cut(s) 113, 719, 1104, 1739, 2069, 4605, 4613
BstMWI GCNNNNNNNGC 9 cut(s) 1010, 1125, 1493, 2045, 2234, 2309, 3563, 4166, 4602
BstNI CCWGG 8 cut(s) 1284, 1938, 2373, 2445, 3925, 3996, 4616, 4624
BstNSI RCATGY 9 cut(s) 510, 613, 1222, 1725, 2344, 2461, 3235, 4020, 4223
BstSCI CCNGG 9 cut(s) 1282, 1743, 1936, 2371, 2443, 3923, 3994, 4614, 4622
BstSFI CTRYAG 6 cut(s) 282, 1356, 2616, 3045, 3558, 4312
BstSLI GKGCMC 2 cut(s) 2823, 4535
BstSNI TACGTA 1 cut(s) 1627
BstUI CGCG 1 cut(s) 2256
BstV1I GCAGC 6 cut(s) 1342, 2240, 2991, 3194, 3544, 4487
BstV2I GAAGAC 2 cut(s) 3482, 3648
BstX2I RGATCY 2 cut(s) 543, 2148
BstXI CCANNNNNNTGG 1 cut(s) 4072
BstYI RGATCY 2 cut(s) 543, 2148
Bsu15I ATCGAT 1 cut(s) 3536
BsuI GTATCC 2 cut(s) 1281, 1616
BsuRI GGCC 6 cut(s) 402, 552, 1192, 2709, 2821, 3221
BsuTUI ATCGAT 1 cut(s) 3536
BtgI CCRYGG 2 cut(s) 547, 1852
BtgZI GCGATG 1 cut(s) 4510
BtsI GCAGTG 4 cut(s) 291, 1391, 2244, 3819
BtsIMutI CAGTG 9 cut(s) 81, 291, 1391, 2244, 2595, 3739, 3819, 4111, 4598
CaiI CAGNNNCTG 1 cut(s) 3413
CfoI GCGC 2 cut(s) 3690, 4279
Cfr10I RCCGGY 1 cut(s) 2224
Cfr13I GGNCC 5 cut(s) 400, 580, 2376, 2819, 2820
ClaI ATCGAT 1 cut(s) 3536
CseI GACGC 1 cut(s) 527
CspCI CAANNNNNGTGG 3 cut(s) 2485, 2520, 4647
Eam1104I CTCTTC 1 cut(s) 2024
EarI CTCTTC 1 cut(s) 2024
EciI GGCGGA 2 cut(s) 3483, 4014
Ecl136II GAGCTC 2 cut(s) 1762, 2161
Eco105I TACGTA 1 cut(s) 1627
Eco130I CCWWGG 6 cut(s) 547, 1187, 1852, 2764, 2854, 4209
Eco24I GRGCYC 4 cut(s) 497, 1764, 2163, 2823
Eco31I GGTCTC 2 cut(s) 4605, 4613
Eco32I GATATC 2 cut(s) 90, 2933
Eco47I GGWCC 2 cut(s) 580, 2376
Eco53kI GAGCTC 2 cut(s) 1762, 2161
Eco57I CTGAAG 3 cut(s) 2031, 2283, 3601
Eco88I CYCGRG 1 cut(s) 4334
EcoICRI GAGCTC 2 cut(s) 1762, 2161
EcoO109I RGGNCCY 1 cut(s) 2376
EcoRI GAATTC 2 cut(s) 976, 991
EcoRII CCWGG 8 cut(s) 1282, 1936, 2371, 2443, 3923, 3994, 4614, 4622
EcoRV GATATC 2 cut(s) 90, 2933
EcoT14I CCWWGG 6 cut(s) 547, 1187, 1852, 2764, 2854, 4209
EcoT22I ATGCAT 3 cut(s) 246, 643, 1560
EcoT38I GRGCYC 4 cut(s) 497, 1764, 2163, 2823
ErhI CCWWGG 6 cut(s) 547, 1187, 1852, 2764, 2854, 4209
Esp3I CGTCTC 1 cut(s) 1739
FalI AAGNNNNNCTT 4 cut(s) 1656, 1688, 3676, 3708
FaqI GGGAC 4 cut(s) 1374, 2389, 2905, 3268
FauI CCCGC 6 cut(s) 193, 267, 336, 1399, 1489, 3618
FauNDI CATATG 2 cut(s) 1399, 2199
FbaI TGATCA 2 cut(s) 3279, 3360
FblI GTMKAC 1 cut(s) 1974
FriOI GRGCYC 4 cut(s) 497, 1764, 2163, 2823
FspBI CTAG 8 cut(s) 1908, 2180, 2471, 2711, 2850, 3071, 4004, 4598
FspI TGCGCA 1 cut(s) 4278
GlaI GCGC 2 cut(s) 3689, 4278
GsuI CTGGAG 7 cut(s) 956, 1734, 2466, 3946, 4627, 4637, 4645
HaeIII GGCC 6 cut(s) 402, 552, 1192, 2709, 2821, 3221
HapII CCGG 4 cut(s) 1631, 1744, 2225, 3895
HgaI GACGC 1 cut(s) 527
HhaI GCGC 2 cut(s) 3690, 4279
Hin1I GRCGYC 1 cut(s) 538
Hin6I GCGC 2 cut(s) 3688, 4277
HinP1I GCGC 2 cut(s) 3688, 4277
HincII GTYRAC 4 cut(s) 1216, 2131, 4441, 4587
HindII GTYRAC 4 cut(s) 1216, 2131, 4441, 4587
HindIII AAGCTT 5 cut(s) 1919, 1960, 2354, 2771, 3853
HpaII CCGG 4 cut(s) 1631, 1744, 2225, 3895
HphI GGTGA 9 cut(s) 886, 1559, 1785, 3660, 3688, 3737, 3809, 3842, 4654
HpyCH4IV ACGT 4 cut(s) 380, 485, 1626, 1732
HpyF10VI GCNNNNNNNGC 9 cut(s) 1010, 1125, 1493, 2045, 2234, 2309, 3563, 4166, 4602
HpySE526I ACGT 4 cut(s) 380, 485, 1626, 1732
Hsp92I GRCGYC 1 cut(s) 538
HspAI GCGC 2 cut(s) 3688, 4277
KflI GGGWCCC 1 cut(s) 2376
Ksp22I TGATCA 2 cut(s) 3279, 3360
LmnI GCTCC 4 cut(s) 112, 1034, 1645, 2165
Lsp1109I GCAGC 6 cut(s) 1342, 2240, 2991, 3194, 3544, 4487
LweI GCATC 9 cut(s) 19, 231, 628, 1567, 2991, 3994, 4487, 4500, 4663
MaeI CTAG 8 cut(s) 1908, 2180, 2471, 2711, 2850, 3071, 4004, 4598
MaeII ACGT 4 cut(s) 380, 485, 1626, 1732
MbiI CCGCTC 1 cut(s) 274
MfeI CAATTG 5 cut(s) 2232, 2973, 3153, 3783, 4251
MflI RGATCY 2 cut(s) 543, 2148
MhlI GDGCHC 6 cut(s) 497, 1764, 2163, 2823, 4335, 4535
MlyI GAGTC 6 cut(s) 161, 665, 1192, 1652, 2041, 4107
MmeI TCCRAC 5 cut(s) 129, 1487, 2517, 3261, 4647
Mph1103I ATGCAT 3 cut(s) 246, 643, 1560
MroXI GAANNNNTTC 1 cut(s) 2626
MslI CAYNNNNRTG 5 cut(s) 962, 1023, 1384, 1769, 4508
MspA1I CMGCKG 3 cut(s) 2398, 2926, 4199
MspCI CTTAAG 1 cut(s) 2701
MspI CCGG 4 cut(s) 1631, 1744, 2225, 3895
MspR9I CCNGG 9 cut(s) 1284, 1745, 1938, 2373, 2445, 3925, 3996, 4616, 4624
MunI CAATTG 5 cut(s) 2232, 2973, 3153, 3783, 4251
Mva1269I GAATGC 6 cut(s) 298, 481, 1323, 1909, 4405, 4503
MvaI CCWGG 8 cut(s) 1284, 1938, 2373, 2445, 3925, 3996, 4616, 4624
MvnI CGCG 1 cut(s) 2256
MwoI GCNNNNNNNGC 9 cut(s) 1010, 1125, 1493, 2045, 2234, 2309, 3563, 4166, 4602
NciI CCSGG 1 cut(s) 1745
NcoI CCATGG 2 cut(s) 547, 1852
NdeI CATATG 2 cut(s) 1399, 2199
NheI GCTAGC 1 cut(s) 1907
NlaIV GGNNCC 9 cut(s) 114, 471, 1030, 1850, 2377, 2378, 2821, 3328, 3760
NmeAIII GCCGAG 1 cut(s) 1998
NmuCI GTSAC 4 cut(s) 892, 1655, 3830, 4115
NsbI TGCGCA 1 cut(s) 4278
NsiI ATGCAT 3 cut(s) 246, 643, 1560
NspI RCATGY 9 cut(s) 510, 613, 1222, 1725, 2344, 2461, 3235, 4020, 4223
NspV TTCGAA 2 cut(s) 3092, 3105
OliI CACNNNNGTG 1 cut(s) 1384
PaeI GCATGC 1 cut(s) 2461
PaeR7I CTCGAG 1 cut(s) 4334
PciI ACATGT 6 cut(s) 609, 1218, 2340, 3231, 4016, 4219
PctI GAATGC 6 cut(s) 298, 481, 1323, 1909, 4405, 4503
PdmI GAANNNNTTC 1 cut(s) 2626
PfeI GAWTC 9 cut(s) 648, 941, 1334, 2650, 2896, 3266, 3367, 3629, 3898
PflMI CCANNNNNTGG 2 cut(s) 3413, 4429
PfoI TCCNGGA 1 cut(s) 2443
PleI GAGTC 6 cut(s) 160, 664, 1191, 1652, 2040, 4106
PpsI GAGTC 6 cut(s) 160, 664, 1191, 1652, 2040, 4106
Ppu21I YACGTR 2 cut(s) 381, 1627
PpuMI RGGWCCY 1 cut(s) 2376
PscI ACATGT 6 cut(s) 609, 1218, 2340, 3231, 4016, 4219
PsiI TTATAA 1 cut(s) 882
Psp124BI GAGCTC 2 cut(s) 1764, 2163
Psp5II RGGWCCY 1 cut(s) 2376
Psp6I CCWGG 8 cut(s) 1282, 1936, 2371, 2443, 3923, 3994, 4614, 4622
PspGI CCWGG 8 cut(s) 1282, 1936, 2371, 2443, 3923, 3994, 4614, 4622
PspN4I GGNNCC 9 cut(s) 114, 471, 1030, 1850, 2377, 2378, 2821, 3328, 3760
PspOMI GGGCCC 1 cut(s) 2819
PspPI GGNCC 5 cut(s) 400, 580, 2376, 2819, 2820
PspPPI RGGWCCY 1 cut(s) 2376
PspXI VCTCGAGB 1 cut(s) 4334
PstI CTGCAG 3 cut(s) 286, 1360, 3562
PstNI CAGNNNCTG 1 cut(s) 3413
PsuI RGATCY 2 cut(s) 543, 2148
PvuII CAGCTG 3 cut(s) 2398, 2926, 4199
RseI CAYNNNNRTG 5 cut(s) 962, 1023, 1384, 1769, 4508
SacI GAGCTC 2 cut(s) 1764, 2163
Sau96I GGNCC 5 cut(s) 400, 580, 2376, 2819, 2820
SchI GAGTC 6 cut(s) 161, 665, 1192, 1652, 2041, 4107
ScrFI CCNGG 9 cut(s) 1284, 1745, 1938, 2373, 2445, 3925, 3996, 4616, 4624
SduI GDGCHC 6 cut(s) 497, 1764, 2163, 2823, 4335, 4535
SfaNI GCATC 9 cut(s) 19, 231, 628, 1567, 2991, 3994, 4487, 4500, 4663
SfcI CTRYAG 6 cut(s) 282, 1356, 2616, 3045, 3558, 4312
Sfr274I CTCGAG 1 cut(s) 4334
SfuI TTCGAA 2 cut(s) 3092, 3105
SinI GGWCC 2 cut(s) 580, 2376
SlaI CTCGAG 1 cut(s) 4334
SmiMI CAYNNNNRTG 5 cut(s) 962, 1023, 1384, 1769, 4508
SmlI CTYRAG 6 cut(s) 1757, 2318, 2701, 4334, 4490, 4514
SmoI CTYRAG 6 cut(s) 1757, 2318, 2701, 4334, 4490, 4514
SnaBI TACGTA 1 cut(s) 1627
SphI GCATGC 1 cut(s) 2461
SspI AATATT 1 cut(s) 3427
SspMI CTAG 8 cut(s) 1908, 2180, 2471, 2711, 2850, 3071, 4004, 4598
SstI GAGCTC 2 cut(s) 1764, 2163
StyD4I CCNGG 9 cut(s) 1282, 1743, 1936, 2371, 2443, 3923, 3994, 4614, 4622
StyI CCWWGG 6 cut(s) 547, 1187, 1852, 2764, 2854, 4209
TaiI ACGT 4 cut(s) 383, 488, 1629, 1735
TaqII GACCGA 1 cut(s) 4372
TatI WGTACW 7 cut(s) 3, 915, 2337, 2721, 2860, 3145, 3929
TauI GCSGC 4 cut(s) 1007, 2511, 2709, 3060
TfiI GAWTC 9 cut(s) 648, 941, 1334, 2650, 2896, 3266, 3367, 3629, 3898
TscAI CASTG 9 cut(s) 88, 291, 1391, 2251, 2602, 3739, 3819, 4118, 4598
TseFI GTSAC 4 cut(s) 892, 1655, 3830, 4115
TseI GCWGC 6 cut(s) 1355, 2228, 3004, 3207, 3557, 4500
Tsp45I GTSAC 4 cut(s) 892, 1655, 3830, 4115
TspGWI ACGGA 1 cut(s) 2117
TspRI CASTG 9 cut(s) 88, 291, 1391, 2251, 2602, 3739, 3819, 4118, 4598
Van91I CCANNNNNTGG 2 cut(s) 3413, 4429
Vha464I CTTAAG 1 cut(s) 2701
VneI GTGCAC 1 cut(s) 4531
VpaK11BI GGWCC 2 cut(s) 580, 2376
XceI RCATGY 9 cut(s) 510, 613, 1222, 1725, 2344, 2461, 3235, 4020, 4223
XhoI CTCGAG 1 cut(s) 4334
XmiI GTMKAC 1 cut(s) 1974
XmnI GAANNNNTTC 1 cut(s) 2626
XspI CTAG 8 cut(s) 1908, 2180, 2471, 2711, 2850, 3071, 4004, 4598
Zsp2I ATGCAT 3 cut(s) 246, 643, 1560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.