FvH4_6g18350

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017692
Physical Location & Seq
Reverse (-)
12114657 .. 12116810
2154 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18350.t1

Sequence Viewer

Length: 1092 bp
ATGGAACTAACTAAGTCTGCTCAAAGGCTATTCCTACCTCTATGTCTGGTCTCTTTTTTCGTTTCAGGAGAAGGAGGGGCACGGCAGAAAGTTTCAGCTGTTGCTCAGCAAGACTGGCGTCCGACAAAGCTGTTTGTTTTTGGAGACTCCTTTGCTGCCACAGGCAACAATGACAATGATTTTTTGAACACCCCTTGGAATTATCCTTATGGAAAGACCTTCCCCGGAAAAGCAACCGGCCGATACTCCGACGGTCGTGTCCTCACTGATTTCATTGCCAAGTTCATAGGAGTGAAGTCTCCGATCTCATACAAATTAAAAAATAAAGTTGCAGGTGATTATTTGAAATACGGGATGAACTTCGCATACGCAGCTACGGGTGTGACCAGTATCTCATACTATTACCCCAACTTGACTACCCAAATAGACTACTTTCAGCGGATTATCGAGGGAGCAAGTTCTATATACAACACCACAGACCTCCACTCTTCTGTGGCTCTCGTTACCGTCTCCGGTGATGACTATGAGTCTTACATTTTCAGTATGGAAGGCCATACTCCGGCCTATCAATTACAGAGTTGGAAATCCTTCAGCACCTCAGTAGTAAATCAGATAACCATTAGTTTAAAACGTATCCATGACTTGGGAGTTAAGAAAATACTCGTGACTGCCCTGGAACCTATGGGATGTCGCCCTACAAGTACGGCTGTATTCTCCTACAAAAAGTGCAATGAAACCAGAAACTCACTTTCCAGGTCTCACAACGTCCTGTTAAAGCAAGCTGTGGCAAAGTTGAACAACCAGGCCAAGAGTTCTTCCTTCATCATTCTCGATCTTTATACCTCTTTCATGTCCGCATTGAAACACAAAGGTACTACAAAGTTTAAGGACGGACTAAAGCCCTGTTGTGCTGGTGTAACACCTGATTATCCATATGGTTGTGGCATGATGGCTGACATACCGGGGGAAGAAACTTATACCATTTGCAAGAGTGCAGCTGCTGCCTTCTTTTGGGACTATGCTCACCCTACACAACAGGGATGGTTCGCTGTGTATTCAAACCTCAAAGCTACACTTAAGAAACTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

364

Amino Acids

40.32

Weight (kDa)

9.24

Isoelectric Point (pI)

23.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 44 - 347 8.8e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017692)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g18350
rosa_chinensis RchiOBHm_Chr3g0472831
rosa_laevigata RLG00000024052
rosa_multiflora Rmu_sc0005608.1_g000009
rosa_roxburghii Rroxscaffold_6G00408570
rosa_rugosa Rorug03G0128000
rosa_samantha Rh3AG180700 Rh3BG206900 Rh3CG204100 Rh3CG222500 Rh3DG169000
rosa_wichuraiana Rw3G016230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 323
AasI GACNNNNNNGTC 1 cut(s) 257
Acc36I ACCTGC 1 cut(s) 323
AccB7I CCANNNNNTGG 1 cut(s) 643
AciI CCGC 2 cut(s) 439, 855
AcoI YGGCCR 1 cut(s) 238
AcuI CTGAAG 1 cut(s) 574
AcyI GRCGYC 1 cut(s) 118
AfaI GTAC 2 cut(s) 703, 874
AfiI CCNNNNNNNGG 3 cut(s) 559, 643, 1011
AflII CTTAAG 1 cut(s) 1076
AgsI TTSAA 5 cut(s) 187, 346, 796, 862, 1059
AhdI GACNNNNNGTC 1 cut(s) 526
AjnI CCWGG 3 cut(s) 672, 752, 801
AluBI AGCT 6 cut(s) 98, 130, 374, 782, 998, 1070
AluI AGCT 6 cut(s) 98, 130, 374, 782, 998, 1070
Alw26I GTCTC 5 cut(s) 55, 138, 303, 514, 762
AlwNI CAGNNNCTG 1 cut(s) 1001
AoxI GGCC 4 cut(s) 238, 550, 561, 804
ApeKI GCWGC 5 cut(s) 155, 371, 995, 998, 1001
Asp700I GAANNNNTTC 2 cut(s) 587, 1085
AsuC2I CCSGG 2 cut(s) 225, 963
AsuHPI GGTGA 3 cut(s) 347, 527, 1016
BaeGI GKGCMC 1 cut(s) 82
BauI CACGAG 1 cut(s) 662
BbvI GCAGC 5 cut(s) 142, 383, 985, 988, 1007
BccI CCATC 2 cut(s) 943, 1035
BceAI ACGGC 2 cut(s) 98, 720
BciT130I CCWGG 3 cut(s) 674, 754, 803
BciVI GTATCC 1 cut(s) 644
BcnI CCSGG 2 cut(s) 225, 963
BcoDI GTCTC 5 cut(s) 55, 138, 303, 514, 762
BfrI CTTAAG 1 cut(s) 1076
BfuAI ACCTGC 1 cut(s) 323
BfuI GTATCC 1 cut(s) 644
BisI GCNGC 5 cut(s) 156, 372, 996, 999, 1002
BlpI GCTNAGC 1 cut(s) 105
BlsI GCNGC 5 cut(s) 157, 373, 997, 1000, 1003
Bme1390I CCNGG 5 cut(s) 225, 674, 754, 803, 963
BmeRI GACNNNNNGTC 1 cut(s) 526
BmiI GGNNCC 1 cut(s) 678
BmrFI CCNGG 5 cut(s) 225, 674, 754, 803, 963
BoxI GACNNNNGTC 1 cut(s) 117
Bpu1102I GCTNAGC 1 cut(s) 105
BpuMI CCSGG 2 cut(s) 225, 963
BsaHI GRCGYC 1 cut(s) 118
BsaI GGTCTC 2 cut(s) 55, 762
BsaJI CCNNGG 4 cut(s) 194, 223, 672, 962
BsaWI WCCGGW 1 cut(s) 512
Bsc4I CCNNNNNNNGG 3 cut(s) 559, 643, 1011
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 2 cut(s) 119, 387
Bse3DI GCAATG 2 cut(s) 273, 736
BseBI CCWGG 3 cut(s) 674, 754, 803
BseDI CCNNGG 4 cut(s) 194, 223, 672, 962
BseGI GGATG 3 cut(s) 360, 692, 1046
BseLI CCNNNNNNNGG 3 cut(s) 559, 643, 1011
BseMI GCAATG 2 cut(s) 273, 736
BseMII CTCAG 2 cut(s) 119, 612
BseNI ACTGG 2 cut(s) 119, 387
BseSI GKGCMC 1 cut(s) 82
BseX3I CGGCCG 1 cut(s) 238
BseXI GCAGC 5 cut(s) 142, 383, 985, 988, 1007
BsgI GTGCAG 1 cut(s) 1014
Bsh1285I CGRYCG 2 cut(s) 241, 256
BshFI GGCC 4 cut(s) 240, 552, 563, 806
BsiEI CGRYCG 2 cut(s) 241, 256
BsiSI CCGG 5 cut(s) 225, 237, 513, 560, 962
BslFI GGGAC 1 cut(s) 1028
BslI CCNNNNNNNGG 3 cut(s) 559, 643, 1011
BsmAI GTCTC 5 cut(s) 55, 138, 303, 514, 762
BsmBI CGTCTC 1 cut(s) 514
BsmFI GGGAC 1 cut(s) 1028
BsnI GGCC 4 cut(s) 240, 552, 563, 806
Bso31I GGTCTC 2 cut(s) 55, 762
Bsp1286I GDGCHC 1 cut(s) 82
Bsp143I GATC 2 cut(s) 303, 832
Bsp1720I GCTNAGC 1 cut(s) 105
BspACI CCGC 2 cut(s) 439, 855
BspANI GGCC 4 cut(s) 240, 552, 563, 806
BspCNI CTCAG 2 cut(s) 118, 611
BspLI GGNNCC 1 cut(s) 678
BspMI ACCTGC 1 cut(s) 323
BspTI CTTAAG 1 cut(s) 1076
BspTNI GGTCTC 2 cut(s) 55, 762
BsrDI GCAATG 2 cut(s) 273, 736
BsrFI RCCGGY 1 cut(s) 236
BsrI ACTGG 2 cut(s) 119, 387
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 4 cut(s) 194, 223, 672, 962
BssMI GATC 2 cut(s) 303, 832
BssNI GRCGYC 1 cut(s) 118
BssSI CACGAG 1 cut(s) 662
BssT1I CCWWGG 1 cut(s) 194
Bst2BI CACGAG 1 cut(s) 662
Bst2UI CCWGG 3 cut(s) 674, 754, 803
Bst4CI ACNGT 2 cut(s) 254, 508
Bst6I CTCTTC 1 cut(s) 493
BstACI GRCGYC 1 cut(s) 118
BstAFI CTTAAG 1 cut(s) 1076
BstAPI GCANNNNNTGC 1 cut(s) 1001
BstC8I GCNNGC 1 cut(s) 780
BstDEI CTNAG 3 cut(s) 12, 105, 598
BstF5I GGATG 3 cut(s) 360, 692, 1046
BstKTI GATC 2 cut(s) 306, 835
BstMAI GTCTC 5 cut(s) 55, 138, 303, 514, 762
BstMBI GATC 2 cut(s) 303, 832
BstMCI CGRYCG 2 cut(s) 241, 256
BstMWI GCNNNNNNNGC 3 cut(s) 115, 371, 1001
BstNI CCWGG 3 cut(s) 674, 754, 803
BstPAI GACNNNNGTC 1 cut(s) 117
BstSCI CCNGG 5 cut(s) 223, 672, 752, 801, 961
BstSLI GKGCMC 1 cut(s) 82
BstV1I GCAGC 5 cut(s) 142, 383, 985, 988, 1007
BstZI CGGCCG 1 cut(s) 238
BsuI GTATCC 1 cut(s) 644
BsuRI GGCC 4 cut(s) 240, 552, 563, 806
BtsCI GGATG 3 cut(s) 360, 692, 1046
BtsIMutI CAGTG 1 cut(s) 264
BveI ACCTGC 1 cut(s) 323
Cac8I GCNNGC 1 cut(s) 780
CaiI CAGNNNCTG 1 cut(s) 1001
Cfr10I RCCGGY 1 cut(s) 236
CseI GACGC 1 cut(s) 107
Csp6I GTAC 2 cut(s) 702, 873
CviAII CATG 3 cut(s) 638, 850, 946
CviQI GTAC 2 cut(s) 702, 873
DdeI CTNAG 3 cut(s) 12, 105, 598
DpnI GATC 2 cut(s) 305, 834
DpnII GATC 2 cut(s) 303, 832
DraI TTTAAA 1 cut(s) 627
DrdI GACNNNNNNGTC 1 cut(s) 257
DriI GACNNNNNGTC 1 cut(s) 526
DseDI GACNNNNNNGTC 1 cut(s) 257
EaeI YGGCCR 1 cut(s) 238
EagI CGGCCG 1 cut(s) 238
Eam1104I CTCTTC 1 cut(s) 493
Eam1105I GACNNNNNGTC 1 cut(s) 526
EarI CTCTTC 1 cut(s) 493
EclXI CGGCCG 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 194
Eco31I GGTCTC 2 cut(s) 55, 762
Eco52I CGGCCG 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 574
EcoRII CCWGG 3 cut(s) 672, 752, 801
EcoT14I CCWWGG 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 194
Esp3I CGTCTC 1 cut(s) 514
FaeI CATG 3 cut(s) 641, 853, 949
FalI AAGNNNNNCTT 3 cut(s) 1059, 1091, 1070
FaqI GGGAC 1 cut(s) 1028
FatI CATG 3 cut(s) 637, 849, 945
FauNDI CATATG 1 cut(s) 934
Fnu4HI GCNGC 5 cut(s) 156, 372, 996, 999, 1002
FokI GGATG 3 cut(s) 367, 699, 1053
Fsp4HI GCNGC 5 cut(s) 156, 372, 996, 999, 1002
GluI GCNGC 5 cut(s) 156, 372, 996, 999, 1002
HaeIII GGCC 4 cut(s) 240, 552, 563, 806
HapII CCGG 5 cut(s) 225, 237, 513, 560, 962
HgaI GACGC 1 cut(s) 107
Hin1I GRCGYC 1 cut(s) 118
Hin1II CATG 3 cut(s) 641, 853, 949
HinfI GANTC 2 cut(s) 146, 527
HpaII CCGG 5 cut(s) 225, 237, 513, 560, 962
HphI GGTGA 3 cut(s) 347, 527, 1016
Hpy188I TCNGA 4 cut(s) 123, 250, 303, 612
Hpy188III TCNNGA 3 cut(s) 66, 664, 830
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 6 cut(s) 65, 229, 542, 598, 829, 1015
HpyCH4III ACNGT 2 cut(s) 254, 508
HpyCH4IV ACGT 2 cut(s) 631, 765
HpyCH4V TGCA 4 cut(s) 332, 729, 987, 995
HpyF10VI GCNNNNNNNGC 3 cut(s) 115, 371, 1001
HpyF3I CTNAG 3 cut(s) 12, 105, 598
HpySE526I ACGT 2 cut(s) 631, 765
Hsp92I GRCGYC 1 cut(s) 118
Hsp92II CATG 3 cut(s) 641, 853, 949
Kzo9I GATC 2 cut(s) 303, 832
LmnI GCTCC 1 cut(s) 452
Lsp1109I GCAGC 5 cut(s) 142, 383, 985, 988, 1007
MaeII ACGT 2 cut(s) 631, 765
MaeIII GTNAC 4 cut(s) 382, 502, 664, 916
MalI GATC 2 cut(s) 305, 834
MboI GATC 2 cut(s) 303, 832
MboII GAAGA 4 cut(s) 480, 807, 980, 1078
MhlI GDGCHC 1 cut(s) 82
MluCI AATT 3 cut(s) 199, 314, 569
MlyI GAGTC 2 cut(s) 140, 536
MmeI TCCRAC 3 cut(s) 146, 273, 560
MnlI CCTC 8 cut(s) 48, 68, 272, 442, 491, 607, 853, 1073
MroXI GAANNNNTTC 2 cut(s) 587, 1085
MseI TTAA 6 cut(s) 317, 626, 651, 773, 885, 1077
MslI CAYNNNNRTG 1 cut(s) 290
MspA1I CMGCKG 3 cut(s) 98, 439, 998
MspCI CTTAAG 1 cut(s) 1076
MspI CCGG 5 cut(s) 225, 237, 513, 560, 962
MspR9I CCNGG 5 cut(s) 225, 674, 754, 803, 963
MvaI CCWGG 3 cut(s) 674, 754, 803
MwoI GCNNNNNNNGC 3 cut(s) 115, 371, 1001
NciI CCSGG 2 cut(s) 225, 963
NdeI CATATG 1 cut(s) 934
NdeII GATC 2 cut(s) 303, 832
NlaIII CATG 3 cut(s) 641, 853, 949
NlaIV GGNNCC 1 cut(s) 678
NmuCI GTSAC 2 cut(s) 382, 664
PaqCI CACCTGC 1 cut(s) 323
PdmI GAANNNNTTC 2 cut(s) 587, 1085
PflMI CCANNNNNTGG 1 cut(s) 643
PkrI GCNGC 5 cut(s) 157, 373, 997, 1000, 1003
PleI GAGTC 2 cut(s) 140, 535
PpsI GAGTC 2 cut(s) 140, 535
PshAI GACNNNNGTC 1 cut(s) 117
Psp6I CCWGG 3 cut(s) 672, 752, 801
PspGI CCWGG 3 cut(s) 672, 752, 801
PspN4I GGNNCC 1 cut(s) 678
PsrI GAACNNNNNNTAC 2 cut(s) 350, 382
PstNI CAGNNNCTG 1 cut(s) 1001
PvuII CAGCTG 2 cut(s) 98, 998
RsaI GTAC 2 cut(s) 703, 874
RsaNI GTAC 2 cut(s) 702, 873
RseI CAYNNNNRTG 1 cut(s) 290
SaqAI TTAA 6 cut(s) 317, 626, 651, 773, 885, 1077
SatI GCNGC 5 cut(s) 156, 372, 996, 999, 1002
Sau3AI GATC 2 cut(s) 303, 832
SchI GAGTC 2 cut(s) 140, 536
ScrFI CCNGG 5 cut(s) 225, 674, 754, 803, 963
SduI GDGCHC 1 cut(s) 82
SmiMI CAYNNNNRTG 1 cut(s) 290
SmlI CTYRAG 1 cut(s) 1076
SmoI CTYRAG 1 cut(s) 1076
Sse9I AATT 3 cut(s) 199, 314, 569
SsiI CCGC 2 cut(s) 439, 855
StyD4I CCNGG 5 cut(s) 223, 672, 752, 801, 961
StyI CCWWGG 1 cut(s) 194
TaaI ACNGT 2 cut(s) 254, 508
TaiI ACGT 2 cut(s) 634, 768
TaqI TCGA 2 cut(s) 447, 831
TasI AATT 3 cut(s) 199, 314, 569
Tru1I TTAA 6 cut(s) 317, 626, 651, 773, 885, 1077
Tru9I TTAA 6 cut(s) 317, 626, 651, 773, 885, 1077
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 2 cut(s) 382, 664
TseI GCWGC 5 cut(s) 155, 371, 995, 998, 1001
Tsp45I GTSAC 2 cut(s) 382, 664
TspDTI ATGAA 6 cut(s) 262, 274, 371, 747, 811, 838
TspGWI ACGGA 1 cut(s) 906
TspRI CASTG 1 cut(s) 271
Van91I CCANNNNNTGG 1 cut(s) 643
Vha464I CTTAAG 1 cut(s) 1076
XmnI GAANNNNTTC 2 cut(s) 587, 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.