Rw3G016230

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0017692
Physical Location & Seq
Forward (+)
17009821 .. 17011607
1787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G016230.1

Sequence Viewer

Length: 1080 bp
ATGGAACTAGCTAAGTCAGCTCAAAGGCTCTTCCTTCCTCACTTTCTGATCTTTTGTTTCATTTCAGGAGGGGCACGGCAGCTAGTTTCAGCTACTGTCCAGAGCAATTGGCGGCCAACAAAGCTATTTGTTTTCGGAGACTCCTTTGCTGCCACAGGCAACAATGACAATGACTTTTTGAACATCCCTTGGAATTATCCTTATGGAAAGACCTTTCCAGGAAAAGCAACTGGCCGTTACTCCGATGGTCGTGTTCTCACTGATTTCATTGCCAAGTTCATAGGAGTGAAGTCTCCGATCTCATACAAATTAAAAAATAAAGTTGCAGGCGATTATTTGAAATACGGGATGAATTTCGCATATGCAGACACGGGTGTGACCAGTCTCTCATACTTTTACCCAAACTTGACCACCCAAATAGACTACTTTCAGCGGATTATCGAGGGAGCAAGTTCTGTATACAACACCACAGACCTCCACTCCTCTGTGGCCCTGGTTACCGTTTCCGGTGATGACTATGAGTCTTACATTTTCAGCATGGAGGGTCAGACTCCGAGTTGGAAATCCTTTAGCACATCAATAGTAAATCAGGTAACTACTAGTTTAAAACGTATCCATGACTTGGGAGTGAAGAAAATACTCGTGACTGCCCTGGAACCTATGGGATGTCGCCCTACTGGTACGGCCGTATTCTCATACAAAAAGTGCAATGAAACCCGGAACTCACTTTCCAGGTCTCACAACCTCCTCTTGCAGGAAGCCGTGGCCAAGTTGAACAACCAAACCAAGAGTTCGTCCTTTGTCATTCTTGATCTCTATACGTCCTTCATGTCCGCATTCAAAAACAAAGGAAGCATAAAGTTTAAGGACAGATTAAAGCCATGTTGTGCTAGTGTAACACCCGGATATCCATATGGGTGTGGCACTATGAGTGATATTCCGGGGGAAGATACGTATACCATTTGCGAGAGTGTAGCTGCTGCATTCTTTTGGGACTATGCTCACCCTACACAACAAGGATGGTTTTCTGTGTATTCAAACCTCAAAGCTACACTTAAGAAGCTCTACCAAAATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

359

Amino Acids

40.08

Weight (kDa)

9.16

Isoelectric Point (pI)

22.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 42 - 340 1.6e-22 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017692)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g18350
rosa_chinensis RchiOBHm_Chr3g0472831
rosa_laevigata RLG00000024052
rosa_multiflora Rmu_sc0005608.1_g000009
rosa_roxburghii Rroxscaffold_6G00408570
rosa_rugosa Rorug03G0128000
rosa_samantha Rh3AG180700 Rh3BG206900 Rh3CG204100 Rh3CG222500 Rh3DG169000
rosa_wichuraiana Rw3G016230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 622
AccI GTMKAC 2 cut(s) 459, 956
AciI CCGC 3 cut(s) 112, 433, 834
AcoI YGGCCR 4 cut(s) 113, 232, 684, 765
AcsI RAATTY 1 cut(s) 352
AfaI GTAC 1 cut(s) 682
AfiI CCNNNNNNNGG 2 cut(s) 622, 754
AflII CTTAAG 1 cut(s) 1055
AgsI TTSAA 5 cut(s) 181, 340, 775, 841, 1038
AhdI GACNNNNNGTC 1 cut(s) 520
AhlI ACTAGT 1 cut(s) 599
AjnI CCWGG 4 cut(s) 217, 492, 651, 731
AleI CACNNNNGTG 1 cut(s) 374
AloI GAACNNNNNNTCC 2 cut(s) 713, 745
AluBI AGCT 8 cut(s) 11, 20, 82, 92, 124, 977, 1049, 1063
AluI AGCT 8 cut(s) 11, 20, 82, 92, 124, 977, 1049, 1063
Alw26I GTCTC 4 cut(s) 132, 297, 389, 741
AlwNI CAGNNNCTG 1 cut(s) 95
AoxI GGCC 5 cut(s) 113, 232, 489, 684, 765
ApeKI GCWGC 4 cut(s) 79, 149, 977, 980
ApoI RAATTY 1 cut(s) 352
AspS9I GGNCC 1 cut(s) 490
AsuC2I CCSGG 3 cut(s) 718, 903, 942
AsuHPI GGTGA 2 cut(s) 521, 995
BaeGI GKGCMC 1 cut(s) 76
BaeI ACNNNNGTAYC 2 cut(s) 672, 705
BalI TGGCCA 1 cut(s) 767
BauI CACGAG 1 cut(s) 641
BbvI GCAGC 4 cut(s) 91, 136, 964, 967
BccI CCATC 2 cut(s) 239, 1014
BceAI ACGGC 5 cut(s) 92, 219, 671, 699, 746
BciT130I CCWGG 4 cut(s) 219, 494, 653, 733
BciVI GTATCC 1 cut(s) 623
BcnI CCSGG 3 cut(s) 718, 903, 942
BcoDI GTCTC 4 cut(s) 132, 297, 389, 741
BcuI ACTAGT 1 cut(s) 599
BfaI CTAG 4 cut(s) 8, 83, 600, 891
BfrI CTTAAG 1 cut(s) 1055
BfuI GTATCC 1 cut(s) 623
BisI GCNGC 5 cut(s) 80, 113, 150, 978, 981
BlsI GCNGC 5 cut(s) 81, 114, 151, 979, 982
Bme1390I CCNGG 7 cut(s) 219, 494, 653, 718, 733, 903, 942
BmeRI GACNNNNNGTC 1 cut(s) 520
BmgT120I GGNCC 1 cut(s) 490
BmiI GGNNCC 1 cut(s) 657
BmrFI CCNGG 7 cut(s) 219, 494, 653, 718, 733, 903, 942
BpuMI CCSGG 3 cut(s) 718, 903, 942
BsaAI YACGTR 1 cut(s) 954
BsaI GGTCTC 1 cut(s) 741
BsaJI CCNNGG 5 cut(s) 188, 492, 651, 762, 941
BsaWI WCCGGW 1 cut(s) 506
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bsc4I CCNNNNNNNGG 2 cut(s) 622, 754
Bse1I ACTGG 3 cut(s) 235, 381, 682
Bse3DI GCAATG 2 cut(s) 267, 715
BseBI CCWGG 4 cut(s) 219, 494, 653, 733
BseDI CCNNGG 5 cut(s) 188, 492, 651, 762, 941
BseGI GGATG 4 cut(s) 183, 354, 671, 1025
BseLI CCNNNNNNNGG 2 cut(s) 622, 754
BseMI GCAATG 2 cut(s) 267, 715
BseNI ACTGG 3 cut(s) 235, 381, 682
BseRI GAGGAG 2 cut(s) 472, 737
BseSI GKGCMC 1 cut(s) 76
BseX3I CGGCCG 1 cut(s) 684
BseXI GCAGC 4 cut(s) 91, 136, 964, 967
Bsh1285I CGRYCG 1 cut(s) 687
BshFI GGCC 5 cut(s) 115, 234, 491, 686, 767
BsiEI CGRYCG 1 cut(s) 687
BsiSI CCGG 4 cut(s) 507, 718, 903, 941
BslFI GGGAC 1 cut(s) 1007
BslI CCNNNNNNNGG 2 cut(s) 622, 754
BsmAI GTCTC 4 cut(s) 132, 297, 389, 741
BsmFI GGGAC 1 cut(s) 1007
BsmI GAATGC 2 cut(s) 836, 983
BsnI GGCC 5 cut(s) 115, 234, 491, 686, 767
Bso31I GGTCTC 1 cut(s) 741
Bsp1286I GDGCHC 1 cut(s) 76
Bsp143I GATC 3 cut(s) 48, 297, 811
BspACI CCGC 3 cut(s) 112, 433, 834
BspANI GGCC 5 cut(s) 115, 234, 491, 686, 767
BspLI GGNNCC 1 cut(s) 657
BspQI GCTCTTC 1 cut(s) 35
BspTI CTTAAG 1 cut(s) 1055
BspTNI GGTCTC 1 cut(s) 741
BsrDI GCAATG 2 cut(s) 267, 715
BsrI ACTGG 3 cut(s) 235, 381, 682
BssECI CCNNGG 5 cut(s) 188, 492, 651, 762, 941
BssMI GATC 3 cut(s) 48, 297, 811
BssNAI GTATAC 2 cut(s) 460, 957
BssSI CACGAG 1 cut(s) 641
BssT1I CCWWGG 1 cut(s) 188
Bst1107I GTATAC 2 cut(s) 460, 957
Bst2BI CACGAG 1 cut(s) 641
Bst2UI CCWGG 4 cut(s) 219, 494, 653, 733
Bst4CI ACNGT 2 cut(s) 97, 502
Bst6I CTCTTC 1 cut(s) 35
BstAFI CTTAAG 1 cut(s) 1055
BstBAI YACGTR 1 cut(s) 954
BstC8I GCNNGC 1 cut(s) 328
BstDEI CTNAG 1 cut(s) 12
BstDSI CCRYGG 1 cut(s) 762
BstEII GGTNACC 1 cut(s) 496
BstENI CCTNNNNNAGG 1 cut(s) 752
BstF5I GGATG 4 cut(s) 183, 354, 671, 1025
BstKTI GATC 3 cut(s) 51, 300, 814
BstMAI GTCTC 4 cut(s) 132, 297, 389, 741
BstMBI GATC 3 cut(s) 48, 297, 811
BstMCI CGRYCG 1 cut(s) 687
BstMWI GCNNNNNNNGC 2 cut(s) 17, 121
BstNI CCWGG 4 cut(s) 219, 494, 653, 733
BstPI GGTNACC 1 cut(s) 496
BstSCI CCNGG 7 cut(s) 217, 492, 651, 716, 731, 901, 940
BstSLI GKGCMC 1 cut(s) 76
BstSNI TACGTA 1 cut(s) 954
BstV1I GCAGC 4 cut(s) 91, 136, 964, 967
BstZ17I GTATAC 2 cut(s) 460, 957
BstZI CGGCCG 1 cut(s) 684
BsuI GTATCC 1 cut(s) 623
BsuRI GGCC 5 cut(s) 115, 234, 491, 686, 767
BtgI CCRYGG 1 cut(s) 762
BtsCI GGATG 4 cut(s) 183, 354, 671, 1025
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 328
CaiI CAGNNNCTG 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 490
Csp6I GTAC 1 cut(s) 681
CviAII CATG 4 cut(s) 538, 617, 829, 882
CviQI GTAC 1 cut(s) 681
DdeI CTNAG 1 cut(s) 12
DpnI GATC 3 cut(s) 50, 299, 813
DpnII GATC 3 cut(s) 48, 297, 811
DraI TTTAAA 1 cut(s) 606
DriI GACNNNNNGTC 1 cut(s) 520
EaeI YGGCCR 4 cut(s) 113, 232, 684, 765
EagI CGGCCG 1 cut(s) 684
Eam1104I CTCTTC 1 cut(s) 35
Eam1105I GACNNNNNGTC 1 cut(s) 520
EarI CTCTTC 1 cut(s) 35
EclXI CGGCCG 1 cut(s) 684
Eco105I TACGTA 1 cut(s) 954
Eco130I CCWWGG 1 cut(s) 188
Eco31I GGTCTC 1 cut(s) 741
Eco32I GATATC 1 cut(s) 908
Eco52I CGGCCG 1 cut(s) 684
Eco91I GGTNACC 1 cut(s) 496
EcoNI CCTNNNNNAGG 1 cut(s) 752
EcoO65I GGTNACC 1 cut(s) 496
EcoRII CCWGG 4 cut(s) 217, 492, 651, 731
EcoRV GATATC 1 cut(s) 908
EcoT14I CCWWGG 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 4 cut(s) 541, 620, 832, 885
FalI AAGNNNNNCTT 2 cut(s) 1038, 1070
FaqI GGGAC 1 cut(s) 1007
FatI CATG 4 cut(s) 537, 616, 828, 881
FauNDI CATATG 2 cut(s) 361, 913
FblI GTMKAC 2 cut(s) 459, 956
Fnu4HI GCNGC 5 cut(s) 80, 113, 150, 978, 981
FokI GGATG 4 cut(s) 170, 361, 678, 1032
Fsp4HI GCNGC 5 cut(s) 80, 113, 150, 978, 981
FspBI CTAG 4 cut(s) 8, 83, 600, 891
GluI GCNGC 5 cut(s) 80, 113, 150, 978, 981
HaeIII GGCC 5 cut(s) 115, 234, 491, 686, 767
HapII CCGG 4 cut(s) 507, 718, 903, 941
Hin1II CATG 4 cut(s) 541, 620, 832, 885
HinfI GANTC 3 cut(s) 140, 521, 550
HpaII CCGG 4 cut(s) 507, 718, 903, 941
HphI GGTGA 2 cut(s) 521, 995
Hpy166II GTNNAC 2 cut(s) 460, 957
Hpy188I TCNGA 6 cut(s) 48, 137, 244, 297, 549, 555
Hpy188III TCNNGA 4 cut(s) 66, 100, 643, 809
Hpy8I GTNNAC 2 cut(s) 460, 957
HpyAV CCTTC 2 cut(s) 44, 835
HpyCH4III ACNGT 2 cut(s) 97, 502
HpyCH4IV ACGT 3 cut(s) 610, 821, 953
HpyCH4V TGCA 5 cut(s) 326, 365, 708, 754, 983
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 121
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 3 cut(s) 610, 821, 953
Hsp92II CATG 4 cut(s) 541, 620, 832, 885
Kzo9I GATC 3 cut(s) 48, 297, 811
LguI GCTCTTC 1 cut(s) 35
LmnI GCTCC 1 cut(s) 446
Lsp1109I GCAGC 4 cut(s) 91, 136, 964, 967
MaeI CTAG 4 cut(s) 8, 83, 600, 891
MaeII ACGT 3 cut(s) 610, 821, 953
MaeIII GTNAC 6 cut(s) 236, 376, 496, 592, 643, 895
MalI GATC 3 cut(s) 50, 299, 813
MboI GATC 3 cut(s) 48, 297, 811
MboII GAAGA 3 cut(s) 22, 643, 959
MfeI CAATTG 1 cut(s) 106
MhlI GDGCHC 1 cut(s) 76
MlsI TGGCCA 1 cut(s) 767
MluCI AATT 4 cut(s) 106, 193, 308, 352
MluNI TGGCCA 1 cut(s) 767
MlyI GAGTC 3 cut(s) 134, 530, 544
MmeI TCCRAC 1 cut(s) 539
MnlI CCTC 9 cut(s) 48, 62, 436, 485, 493, 535, 755, 758, 1052
Mox20I TGGCCA 1 cut(s) 767
MscI TGGCCA 1 cut(s) 767
MseI TTAA 5 cut(s) 311, 605, 864, 875, 1056
MslI CAYNNNNRTG 3 cut(s) 284, 374, 916
Msp20I TGGCCA 1 cut(s) 767
MspA1I CMGCKG 1 cut(s) 433
MspCI CTTAAG 1 cut(s) 1055
MspI CCGG 4 cut(s) 507, 718, 903, 941
MspR9I CCNGG 7 cut(s) 219, 494, 653, 718, 733, 903, 942
MunI CAATTG 1 cut(s) 106
Mva1269I GAATGC 2 cut(s) 836, 983
MvaI CCWGG 4 cut(s) 219, 494, 653, 733
MwoI GCNNNNNNNGC 2 cut(s) 17, 121
NciI CCSGG 3 cut(s) 718, 903, 942
NdeI CATATG 2 cut(s) 361, 913
NdeII GATC 3 cut(s) 48, 297, 811
NlaIII CATG 4 cut(s) 541, 620, 832, 885
NlaIV GGNNCC 1 cut(s) 657
NmuCI GTSAC 2 cut(s) 376, 643
OliI CACNNNNGTG 1 cut(s) 374
PciSI GCTCTTC 1 cut(s) 35
PctI GAATGC 2 cut(s) 836, 983
PflMI CCANNNNNTGG 1 cut(s) 622
PfoI TCCNGGA 1 cut(s) 217
PkrI GCNGC 5 cut(s) 81, 114, 151, 979, 982
PleI GAGTC 3 cut(s) 134, 529, 544
PpsI GAGTC 3 cut(s) 134, 529, 544
Ppu21I YACGTR 1 cut(s) 954
Psp6I CCWGG 4 cut(s) 217, 492, 651, 731
PspEI GGTNACC 1 cut(s) 496
PspGI CCWGG 4 cut(s) 217, 492, 651, 731
PspN4I GGNNCC 1 cut(s) 657
PspPI GGNCC 1 cut(s) 490
PstNI CAGNNNCTG 1 cut(s) 95
RsaI GTAC 1 cut(s) 682
RsaNI GTAC 1 cut(s) 681
RseI CAYNNNNRTG 3 cut(s) 284, 374, 916
SapI GCTCTTC 1 cut(s) 35
SaqAI TTAA 5 cut(s) 311, 605, 864, 875, 1056
SatI GCNGC 5 cut(s) 80, 113, 150, 978, 981
Sau3AI GATC 3 cut(s) 48, 297, 811
Sau96I GGNCC 1 cut(s) 490
SchI GAGTC 3 cut(s) 134, 530, 544
ScrFI CCNGG 7 cut(s) 219, 494, 653, 718, 733, 903, 942
SduI GDGCHC 1 cut(s) 76
SmiMI CAYNNNNRTG 3 cut(s) 284, 374, 916
SmlI CTYRAG 1 cut(s) 1055
SmoI CTYRAG 1 cut(s) 1055
SnaBI TACGTA 1 cut(s) 954
SpeI ACTAGT 1 cut(s) 599
Sse9I AATT 4 cut(s) 106, 193, 308, 352
SsiI CCGC 3 cut(s) 112, 433, 834
SspMI CTAG 4 cut(s) 8, 83, 600, 891
StyD4I CCNGG 7 cut(s) 217, 492, 651, 716, 731, 901, 940
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 2 cut(s) 97, 502
TaiI ACGT 3 cut(s) 613, 824, 956
TaqI TCGA 1 cut(s) 441
TasI AATT 4 cut(s) 106, 193, 308, 352
TauI GCSGC 1 cut(s) 115
Tru1I TTAA 5 cut(s) 311, 605, 864, 875, 1056
Tru9I TTAA 5 cut(s) 311, 605, 864, 875, 1056
TscAI CASTG 1 cut(s) 265
TseFI GTSAC 2 cut(s) 376, 643
TseI GCWGC 4 cut(s) 79, 149, 977, 980
Tsp45I GTSAC 2 cut(s) 376, 643
TspDTI ATGAA 6 cut(s) 49, 256, 268, 365, 726, 817
TspRI CASTG 1 cut(s) 265
Van91I CCANNNNNTGG 1 cut(s) 622
Vha464I CTTAAG 1 cut(s) 1055
XagI CCTNNNNNAGG 1 cut(s) 752
XapI RAATTY 1 cut(s) 352
XmiI GTMKAC 2 cut(s) 459, 956
XspI CTAG 4 cut(s) 8, 83, 600, 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.