Rh3CG222500

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0017692
Physical Location & Seq
Reverse (-)
20760965 .. 20763149
2185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG222500.1

Sequence Viewer

Length: 912 bp
ATGGAACTAGCTAAGTCAGCTCAAAGGCTCTTCCTTCCTCTCTTTCCGATCTTTTGTTTCATTTCAGGAGAAGGAGGGGCACGGCAGCTAGTTTCAGCTACTGTCCAGAGCAATTGGCGGCCAACAAAGCTATTTGTTTTCGGAGACTCCTTTGCTGCCACAGGCAACAATGACAATGACTTTTTGAACATCCCTTGGAATTATCCTTATGGAAAGACCTTTCCAGGAAAAGCAACTGGCCGTTACTCCGATGGTCGTGTCCTCACTGATTTCATTGCCAAGTTCATAGGAGTGAAGTCTCCGATCTCATACAAATTAAAAAATAAAGTTGCAGGCGATTATTTGAAATACGGGATGAATTTCGCATATGCAGACACGGGTGTGACCAGTCTGTCATACTTTTACCCAAATTTGACCACTCAAATAGACTACTTTCAGCGGATTATCGAGGGAGCAAGTTCTGTATACAACACCACAGACCTCCACTCCTCTGTGGCCCTGGTTACCGTTTCCGGTGATGACTATGAGTCTTACATTTTCAGCATGGAGGGTCAGACTCCGGCCCATCCATTACAGAGTTGGAAATCCTTTAGCACATCAATAGTAAATGAGGTAACTACTAGTTTAAAACGTATCCATGACTTGGGAGTGAAGAAAATACTCGTGACTGCCCTGGAACCTATGGGATGTCGCCCTACTGGTACGGCCGTATTCTCATACAAAAAGTGCAATGAAACCCGGAACTCACTTTCCAGGTCTCACAACCTCCTCTTGCAGGAAGCCGTGGCCAAGTTGAACAACCAAACCAAGAGGTCGTCCTTTGTCATTCTTGATCTTTATACGTCCTTCATGTCCGCATTCAAAAACAAAGGTAATGTAACATTGAATGTCATTTATTCTAATTACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

303

Amino Acids

33.82

Weight (kDa)

9.29

Isoelectric Point (pI)

30.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 44 - 286 2.1e-19 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017692)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g18350
rosa_chinensis RchiOBHm_Chr3g0472831
rosa_laevigata RLG00000024052
rosa_multiflora Rmu_sc0005608.1_g000009
rosa_roxburghii Rroxscaffold_6G00408570
rosa_rugosa Rorug03G0128000
rosa_samantha Rh3AG180700 Rh3BG206900 Rh3CG204100 Rh3CG222500 Rh3DG169000
rosa_wichuraiana Rw3G016230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 391
AccB7I CCANNNNNTGG 1 cut(s) 643
AccI GTMKAC 1 cut(s) 465
AciI CCGC 3 cut(s) 118, 439, 855
AcoI YGGCCR 4 cut(s) 119, 238, 705, 786
AcsI RAATTY 2 cut(s) 358, 409
AfaI GTAC 1 cut(s) 703
AfiI CCNNNNNNNGG 2 cut(s) 643, 775
AgsI TTSAA 5 cut(s) 187, 346, 796, 862, 886
AhdI GACNNNNNGTC 1 cut(s) 526
AhlI ACTAGT 1 cut(s) 620
AjnI CCWGG 4 cut(s) 223, 498, 672, 752
AleI CACNNNNGTG 1 cut(s) 380
AloI GAACNNNNNNTCC 2 cut(s) 734, 766
AluBI AGCT 5 cut(s) 11, 20, 88, 98, 130
AluI AGCT 5 cut(s) 11, 20, 88, 98, 130
Alw26I GTCTC 3 cut(s) 138, 303, 762
AlwNI CAGNNNCTG 1 cut(s) 101
AoxI GGCC 6 cut(s) 119, 238, 495, 561, 705, 786
ApeKI GCWGC 2 cut(s) 85, 155
ApoI RAATTY 2 cut(s) 358, 409
AspS9I GGNCC 2 cut(s) 496, 562
AsuC2I CCSGG 1 cut(s) 739
AsuHPI GGTGA 1 cut(s) 527
BaeGI GKGCMC 1 cut(s) 82
BaeI ACNNNNGTAYC 2 cut(s) 693, 726
BalI TGGCCA 1 cut(s) 788
BauI CACGAG 1 cut(s) 662
BbvI GCAGC 2 cut(s) 97, 142
BccI CCATC 2 cut(s) 245, 573
BceAI ACGGC 5 cut(s) 98, 225, 692, 720, 767
BciT130I CCWGG 4 cut(s) 225, 500, 674, 754
BciVI GTATCC 1 cut(s) 644
BcnI CCSGG 1 cut(s) 739
BcoDI GTCTC 3 cut(s) 138, 303, 762
BcuI ACTAGT 1 cut(s) 620
BfaI CTAG 3 cut(s) 8, 89, 621
BfuI GTATCC 1 cut(s) 644
BisI GCNGC 3 cut(s) 86, 119, 156
BlsI GCNGC 3 cut(s) 87, 120, 157
Bme1390I CCNGG 5 cut(s) 225, 500, 674, 739, 754
BmeRI GACNNNNNGTC 1 cut(s) 526
BmgT120I GGNCC 2 cut(s) 496, 562
BmiI GGNNCC 1 cut(s) 678
BmrFI CCNGG 5 cut(s) 225, 500, 674, 739, 754
BpuMI CCSGG 1 cut(s) 739
BsaI GGTCTC 1 cut(s) 762
BsaJI CCNNGG 4 cut(s) 194, 498, 672, 783
BsaWI WCCGGW 1 cut(s) 512
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bsc4I CCNNNNNNNGG 2 cut(s) 643, 775
Bse1I ACTGG 3 cut(s) 241, 387, 703
Bse3DI GCAATG 2 cut(s) 273, 736
BseBI CCWGG 4 cut(s) 225, 500, 674, 754
BseDI CCNNGG 4 cut(s) 194, 498, 672, 783
BseGI GGATG 4 cut(s) 189, 360, 565, 692
BseLI CCNNNNNNNGG 2 cut(s) 643, 775
BseMI GCAATG 2 cut(s) 273, 736
BseNI ACTGG 3 cut(s) 241, 387, 703
BseRI GAGGAG 2 cut(s) 478, 758
BseSI GKGCMC 1 cut(s) 82
BseX3I CGGCCG 1 cut(s) 705
BseXI GCAGC 2 cut(s) 97, 142
Bsh1285I CGRYCG 1 cut(s) 708
BshFI GGCC 6 cut(s) 121, 240, 497, 563, 707, 788
BsiEI CGRYCG 1 cut(s) 708
BsiSI CCGG 3 cut(s) 513, 560, 739
BslI CCNNNNNNNGG 2 cut(s) 643, 775
BsmAI GTCTC 3 cut(s) 138, 303, 762
BsmI GAATGC 1 cut(s) 857
BsnI GGCC 6 cut(s) 121, 240, 497, 563, 707, 788
Bso31I GGTCTC 1 cut(s) 762
Bsp1286I GDGCHC 1 cut(s) 82
Bsp143I GATC 3 cut(s) 48, 303, 832
BspACI CCGC 3 cut(s) 118, 439, 855
BspANI GGCC 6 cut(s) 121, 240, 497, 563, 707, 788
BspLI GGNNCC 1 cut(s) 678
BspQI GCTCTTC 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 762
BsrDI GCAATG 2 cut(s) 273, 736
BsrI ACTGG 3 cut(s) 241, 387, 703
BssECI CCNNGG 4 cut(s) 194, 498, 672, 783
BssMI GATC 3 cut(s) 48, 303, 832
BssNAI GTATAC 1 cut(s) 466
BssSI CACGAG 1 cut(s) 662
BssT1I CCWWGG 1 cut(s) 194
Bst1107I GTATAC 1 cut(s) 466
Bst2BI CACGAG 1 cut(s) 662
Bst2UI CCWGG 4 cut(s) 225, 500, 674, 754
Bst4CI ACNGT 2 cut(s) 103, 508
Bst6I CTCTTC 1 cut(s) 35
BstC8I GCNNGC 1 cut(s) 334
BstDEI CTNAG 1 cut(s) 12
BstDSI CCRYGG 1 cut(s) 783
BstEII GGTNACC 1 cut(s) 502
BstENI CCTNNNNNAGG 1 cut(s) 773
BstF5I GGATG 4 cut(s) 189, 360, 565, 692
BstKTI GATC 3 cut(s) 51, 306, 835
BstMAI GTCTC 3 cut(s) 138, 303, 762
BstMBI GATC 3 cut(s) 48, 303, 832
BstMCI CGRYCG 1 cut(s) 708
BstMWI GCNNNNNNNGC 2 cut(s) 17, 127
BstNI CCWGG 4 cut(s) 225, 500, 674, 754
BstPI GGTNACC 1 cut(s) 502
BstSCI CCNGG 5 cut(s) 223, 498, 672, 737, 752
BstSLI GKGCMC 1 cut(s) 82
BstV1I GCAGC 2 cut(s) 97, 142
BstZ17I GTATAC 1 cut(s) 466
BstZI CGGCCG 1 cut(s) 705
BsuI GTATCC 1 cut(s) 644
BsuRI GGCC 6 cut(s) 121, 240, 497, 563, 707, 788
BtgI CCRYGG 1 cut(s) 783
BtsCI GGATG 4 cut(s) 189, 360, 565, 692
BtsIMutI CAGTG 1 cut(s) 264
Cac8I GCNNGC 1 cut(s) 334
CaiI CAGNNNCTG 1 cut(s) 101
Cfr13I GGNCC 2 cut(s) 496, 562
Csp6I GTAC 1 cut(s) 702
CviAII CATG 3 cut(s) 544, 638, 850
CviQI GTAC 1 cut(s) 702
DdeI CTNAG 1 cut(s) 12
DpnI GATC 3 cut(s) 50, 305, 834
DpnII GATC 3 cut(s) 48, 303, 832
DraI TTTAAA 1 cut(s) 627
DrdI GACNNNNNNGTC 1 cut(s) 391
DriI GACNNNNNGTC 1 cut(s) 526
DseDI GACNNNNNNGTC 1 cut(s) 391
EaeI YGGCCR 4 cut(s) 119, 238, 705, 786
EagI CGGCCG 1 cut(s) 705
Eam1104I CTCTTC 1 cut(s) 35
Eam1105I GACNNNNNGTC 1 cut(s) 526
EarI CTCTTC 1 cut(s) 35
EclXI CGGCCG 1 cut(s) 705
Eco130I CCWWGG 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 762
Eco52I CGGCCG 1 cut(s) 705
Eco91I GGTNACC 1 cut(s) 502
EcoNI CCTNNNNNAGG 1 cut(s) 773
EcoO65I GGTNACC 1 cut(s) 502
EcoRII CCWGG 4 cut(s) 223, 498, 672, 752
EcoT14I CCWWGG 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 194
FaeI CATG 3 cut(s) 547, 641, 853
FatI CATG 3 cut(s) 543, 637, 849
FauNDI CATATG 1 cut(s) 367
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 3 cut(s) 86, 119, 156
FokI GGATG 4 cut(s) 176, 367, 552, 699
Fsp4HI GCNGC 3 cut(s) 86, 119, 156
FspBI CTAG 3 cut(s) 8, 89, 621
GluI GCNGC 3 cut(s) 86, 119, 156
HaeIII GGCC 6 cut(s) 121, 240, 497, 563, 707, 788
HapII CCGG 3 cut(s) 513, 560, 739
Hin1II CATG 3 cut(s) 547, 641, 853
HinfI GANTC 3 cut(s) 146, 527, 556
HpaII CCGG 3 cut(s) 513, 560, 739
HphI GGTGA 1 cut(s) 527
Hpy166II GTNNAC 1 cut(s) 466
Hpy188I TCNGA 5 cut(s) 48, 143, 250, 303, 555
Hpy188III TCNNGA 4 cut(s) 66, 106, 664, 830
Hpy8I GTNNAC 1 cut(s) 466
HpyAV CCTTC 3 cut(s) 44, 65, 856
HpyCH4III ACNGT 2 cut(s) 103, 508
HpyCH4IV ACGT 2 cut(s) 631, 842
HpyCH4V TGCA 4 cut(s) 332, 371, 729, 775
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 127
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 2 cut(s) 631, 842
Hsp92II CATG 3 cut(s) 547, 641, 853
Kzo9I GATC 3 cut(s) 48, 303, 832
LguI GCTCTTC 1 cut(s) 35
LmnI GCTCC 1 cut(s) 452
Lsp1109I GCAGC 2 cut(s) 97, 142
MaeI CTAG 3 cut(s) 8, 89, 621
MaeII ACGT 2 cut(s) 631, 842
MaeIII GTNAC 6 cut(s) 242, 382, 502, 613, 664, 877
MalI GATC 3 cut(s) 50, 305, 834
MboI GATC 3 cut(s) 48, 303, 832
MboII GAAGA 2 cut(s) 22, 664
MfeI CAATTG 1 cut(s) 112
MhlI GDGCHC 1 cut(s) 82
MlsI TGGCCA 1 cut(s) 788
MluCI AATT 6 cut(s) 112, 199, 314, 358, 409, 901
MluNI TGGCCA 1 cut(s) 788
MlyI GAGTC 3 cut(s) 140, 536, 550
MmeI TCCRAC 1 cut(s) 560
Mox20I TGGCCA 1 cut(s) 788
MscI TGGCCA 1 cut(s) 788
MseI TTAA 2 cut(s) 317, 626
MslI CAYNNNNRTG 2 cut(s) 290, 380
Msp20I TGGCCA 1 cut(s) 788
MspA1I CMGCKG 1 cut(s) 439
MspI CCGG 3 cut(s) 513, 560, 739
MspR9I CCNGG 5 cut(s) 225, 500, 674, 739, 754
MunI CAATTG 1 cut(s) 112
Mva1269I GAATGC 1 cut(s) 857
MvaI CCWGG 4 cut(s) 225, 500, 674, 754
MwoI GCNNNNNNNGC 2 cut(s) 17, 127
NciI CCSGG 1 cut(s) 739
NdeI CATATG 1 cut(s) 367
NdeII GATC 3 cut(s) 48, 303, 832
NlaIII CATG 3 cut(s) 547, 641, 853
NlaIV GGNNCC 1 cut(s) 678
NmuCI GTSAC 2 cut(s) 382, 664
OliI CACNNNNGTG 1 cut(s) 380
PciSI GCTCTTC 1 cut(s) 35
PctI GAATGC 1 cut(s) 857
PflMI CCANNNNNTGG 1 cut(s) 643
PfoI TCCNGGA 1 cut(s) 223
PkrI GCNGC 3 cut(s) 87, 120, 157
PleI GAGTC 3 cut(s) 140, 535, 550
PpsI GAGTC 3 cut(s) 140, 535, 550
Psp6I CCWGG 4 cut(s) 223, 498, 672, 752
PspEI GGTNACC 1 cut(s) 502
PspGI CCWGG 4 cut(s) 223, 498, 672, 752
PspN4I GGNNCC 1 cut(s) 678
PspPI GGNCC 2 cut(s) 496, 562
PstNI CAGNNNCTG 1 cut(s) 101
RsaI GTAC 1 cut(s) 703
RsaNI GTAC 1 cut(s) 702
RseI CAYNNNNRTG 2 cut(s) 290, 380
SapI GCTCTTC 1 cut(s) 35
SaqAI TTAA 2 cut(s) 317, 626
SatI GCNGC 3 cut(s) 86, 119, 156
Sau3AI GATC 3 cut(s) 48, 303, 832
Sau96I GGNCC 2 cut(s) 496, 562
SchI GAGTC 3 cut(s) 140, 536, 550
ScrFI CCNGG 5 cut(s) 225, 500, 674, 739, 754
SduI GDGCHC 1 cut(s) 82
SmiMI CAYNNNNRTG 2 cut(s) 290, 380
SpeI ACTAGT 1 cut(s) 620
Sse9I AATT 6 cut(s) 112, 199, 314, 358, 409, 901
SsiI CCGC 3 cut(s) 118, 439, 855
SspMI CTAG 3 cut(s) 8, 89, 621
StyD4I CCNGG 5 cut(s) 223, 498, 672, 737, 752
StyI CCWWGG 1 cut(s) 194
TaaI ACNGT 2 cut(s) 103, 508
TaiI ACGT 2 cut(s) 634, 845
TaqI TCGA 1 cut(s) 447
TasI AATT 6 cut(s) 112, 199, 314, 358, 409, 901
TauI GCSGC 1 cut(s) 121
Tru1I TTAA 2 cut(s) 317, 626
Tru9I TTAA 2 cut(s) 317, 626
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 2 cut(s) 382, 664
TseI GCWGC 2 cut(s) 85, 155
Tsp45I GTSAC 2 cut(s) 382, 664
TspDTI ATGAA 6 cut(s) 49, 262, 274, 371, 747, 838
TspRI CASTG 1 cut(s) 271
Van91I CCANNNNNTGG 1 cut(s) 643
XagI CCTNNNNNAGG 1 cut(s) 773
XapI RAATTY 2 cut(s) 358, 409
XcmI CCANNNNNNNNNTGG 1 cut(s) 576
XmiI GTMKAC 1 cut(s) 465
XspI CTAG 3 cut(s) 8, 89, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.